article: Leprosy remains a public health problem in Brazil with new case incidence exceeding World Health Organization (WHO) goals in endemic clusters throughout the country. Migration can facilitate movement of disease between endemic and non-endemic areas, and has been considered a possible factor in continued leprosy incidence in Brazil. A study was conducted to investigate migration as a risk factor for leprosy. The study had three aims: (1) examine past five year migration as a risk factor for leprosy, (2) describe and compare geographic and temporal patterns of migration among past 5-year migrants with leprosy and a control group, and (3) examine social determinants of health associated with leprosy among past 5-year migrants. The study implemented a matched case-control design and analysis comparing individuals newly diagnosed with leprosy (n = 340) and a clinically unapparent control group (n = 340) without clinical signs of leprosy, matched for age, sex and location in four endemic municipalities in the state of Maranhão, northeastern Brazil. Fishers exact test was used to conduct bivariate analyses. A multivariate logistic regression analysis was employed to control for possible confounding variables. Eighty cases (23. 5%) migrated 5-years prior to diagnosis, and 55 controls (16. 2%) migrated 5-years prior to the corresponding case diagnosis. Past 5 year migration was found to be associated with leprosy (OR: 1. 59; 95% CI 1. 07–2. 38; p = 0. 02), and remained significantly associated with leprosy after controlling for leprosy contact in the family, household, and family/household contact. Poverty, as well as leprosy contact in the family, household and other leprosy contact, was associated with leprosy among past 5-year migrants in the bivariate analysis. Alcohol consumption was also associated with leprosy, a relevant risk factor in susceptibility to infection that should be explored in future research. Our findings provide insight into patterns of migration to localize focused control efforts in endemic areas with high population mobility.
  Leprosy continues to be an endemic disease in many parts of the world. Brazil has globally the second highest new case incidence [1]. National leprosy prevalence of 1. 54/10,000 in 2010 [2] remains above the WHO goal of <1 per 10,000. Highly endemic areas of the disease continue to persist despite large-scale national efforts to control the disease. A challenge in disease control efforts is compounded as leprosy can be diagnosed many years after infection took place due to the long incubation period, and mild early symptoms of the disease may be overlooked. Migration has been found to be a social determinant of disease [3], and has been hypothesized as a risk factor in continued leprosy incidence [4], [5], [6]. In fact, earlier research in Brazil highlighted the increased distribution of leprosy along new corridors coinciding with frontier expansion connecting southern agricultural areas to the north of Brazil [7], as well as periurban migrant settlements on the outskirts of urban centers [4]. Migrants move between endemic and non-endemic areas in Brazil and often live in substandard conditions. As an infectious disease caused by Mycobacterium leprae, leprosy primarily affects the skin and peripheral nerves and causing sensory loss. While nasal mucosa is considered the main transmission site, new research indicates that oral presence of M. leprae bacilli may be an additional mode of transmission [8]. Maranhão, the study area of this research, has the third highest prevalence of leprosy (5. 34/10,000) in the country [2] and is among the states with the highest out- and return- migration rates [9]. The proliferation of leprosy in Brazil continues largely in conditions of poverty that include poor housing and sanitation, high household density, illiteracy and low socioeconomic levels both at the micro and macro levels [4], [10]–[13]. Rapid population growth and uncontrolled urbanization, often as a consequence of migration for employment and differential access to services between rural and urban areas, has facilitated the expansion of these poor social and environmental conditions on the peripheries of cities associated with leprosy infection [4]–[5], [7], [13]. Additionally, new road construction and railways have enabled movement between rural communities and urban areas. These developments in transportation have been argued to explain the expanded distribution of leprosy in Brazil [4]–[6]. Nevertheless, household leprosy contact continues to be the primary risk factor associated with leprosy infection [14]. Proximity to the household contact has been seen as relevant in terms of increased risk [15]. Consanguineous contact has also been found to be associated with leprosy. Findings from Moet et al. (2006) suggest evidence of a genetic relationship independent of physical contact for leprosy infection. Migration has been found to be an impediment to both leprosy elimination and control efforts. Prior research has suggested that migration may influence transmission and distribution of the disease [5], [16] as well as other neglected tropical diseases (NTDs) [3], [17]–[23]. This study explores the spatial and temporal patterns of migration in individuals with leprosy in Maranhão. The study also examines risk factors associated with leprosy among individuals who have migrated in the past five years (past 5-year migrants). Comparison of risks associated with leprosy and migration is challenging in a homogeneous population. However evaluation of specific risk factors that differentiate leprosy among past 5-year migrants from a clinically unapparent control group without clinical signs of leprosy who migrated in the past five years in this investigation, sheds light on those factors that are of importance when considering leprosy infection and expression of disease. The study has three specific aims: 1) to examine if migration in the past five years is a risk factor for leprosy; 2) to describe and compare geographic and temporal patterns of migration among past 5-year migrants with leprosy and a control group without clinical signs of leprosy; 3) to examine the social determinants of health associated with leprosy among past 5-year migrants.
  Written approval was obtained from the Ethical Review Board of the Federal University of Ceará (Fortaleza, Brazil). Permission to perform the study was also obtained by the Maranhão State Health Secretariat, the State Leprosy Control Program and municipalities involved. Informed written consent was obtained from study participants, or their parent/guardian in the case of minors, after explaining the objectives of the study. Interviews were conducted in private. The research was conducted in four leprosy endemic municipalities in the state of Maranhão, Brazil: Santa Inês, São José de Ribamar, Codó, and Bacabal. These municipalities are located in a major endemic cluster identified by the Brazilian Ministry of Health as a high-risk area for leprosy transmission [16]. Santa Inês, (population 77,282) [24], Codó (population 118,038) [24], and Bacabal (population 100,014 same) [24] are small townships in the interior of Maranhão that are largely surrounded by rural agricultural production, while São José de Ribamar (population 163,045) [24] is on the outskirts of the capital city, São Luis. Most households are small brick or mud and palm residences with rudimentary plumbing and hammocks to accommodate the multigenerational inhabitants. A case-control study was designed as part of an extended epidemiological investigation on risk factors associated with leprosy infection in four highly endemic municipalities in Maranhão, as part of the MAPATOPI study. The MAPATOPI study is an interdisciplinary project to support and improve the Brazilian leprosy program in Maranhão, Pará, Tocantins, and Piaui. Variables associated with past five year migration among those diagnosed with leprosy between 2009–2010 were compared with a matched clinically unapparent control group without clinical signs of leprosy. Migration was defined as those who resided outside of the municipality of their current residence, and is limited to five years as this is the average incubation period from leprosy infection to symptom onset. Past five year migration data is also collected in the Brazilian National Household Survey [9]. A detailed analysis of socio-cultural, health service related and economic variables that were collected as part of the larger epidemiological study will be explored elsewhere. The case group was identified through the database of the National Information System for Notifiable Diseases (Sistema de Informação de Agravos de Notificação – SINAN) and included adults 15 and older in each of the four sites diagnosed with leprosy in 2009–2010 (n = 394). Individuals under 15 years of age, those previously diagnosed with leprosy and relapsed, living outside of the highly endemic cluster and who could not be located through multiple contact attempts were excluded from the study. The control group (n = 391) was selected from the Programa Saúde da Família (Program for Family Health). This program registers all families in the catchment areas of the clinic by community health workers. At the clinics, we randomly selected intake forms from the Program for Family Health for age and sex at each clinic and contacted those individuals for inclusion in the control group. Each of the matched controls were clinically evaluated for signs of leprosy. Any individual with a clinical suspicion of leprosy was excluded from the study and referred to municipal health centers for further diagnostic testing. Data collection was conducted between April and August 2010. Data collection was coordinated through the Municipality Health Secretariats with the support of the Maranhão State Health Secretariat. Study participants were recruited by community health agents for the study. They were interviewed by trained health professionals at the local health care centers, or in patient homes when disability or age prevented health center attendance. Information on demographics, socioeconomic status, healthcare access, migration, behavior and stress was collected through structured questionnaires. Clinical data were also collected through patient medical records. Data were entered twice using EpiInfo software version 3. 5. 1 (Centers for Disease Control and Prevention, Atlanta, USA) and cross-checked for entry-related errors. Statistical tests were used to assess normality. Included are data sets with information related to migration. Any cases that did not have complete migration data were excluded from the analysis. Of the 340 leprosy cases and 340 matched controls, we first identified 135 (19. 9%) past 5-year migrants in the case (n = 80) and control groups (n = 55). The distribution of key demographic, spatial and temporal migration pattern variables among past 5-year migrants in the case and control groups was examined and tested by the use of Fishers exact test for significant differences in the stratified sample of past 5-year migrants. We then conducted bivariate analyses comparing cases (n = 340) and controls (n = 340) using Fishers exact test to examine if past five year migration was associated with leprosy diagnosis. As household contact remains the most significant known transmission risk to date for leprosy infection [14], [15], we additionally undertook multivariate logistic regression analysis controlling for family (parent, child and/or sibling) and household (consanguineous and/or non-consanguineous) contact with leprosy. Next, stratified bivariate analyses using Fishers exact tests were used to determine differences in the association among social determinants of health (socioeconomic status), psychosocial (alcohol use and life stressors) and biosocial factors (leprosy contact exposure) for case and control groups of past 5-year migrants (n = 135).
  A total of 394 leprosy cases and 391 controls were interviewed. There were 23 relapsed leprosy cases and 12 controls suspected of leprosy who were excluded from the study. Eight respondents refused to participate. Complete migration data was available for 680 respondents. Of the 340 leprosy cases and 340 matched clinically unapparent controls, 23. 5% of those with leprosy (n = 80 cases) and 16. 2% (n = 55) of the control group without clinical signs of leprosy migrated in the past 5 years before diagnosis. Only 4. 4% (n = 15) of cases migrated after diagnosis. Table 1 reflects migration into and out of major endemic clusters identified by the Brazilian Ministry of Health as high-risk areas for leprosy transmission [6] (Figure 1), and other demographics and migration variables. These variables were not significantly associated with leprosy among past 5-year migrants prior to diagnosis (test results not shown). Leprosy cases were largely among the youngest age group (15–29) migrating, with an equal distribution between males and females. More than one-third of those with leprosy who migrated in the past five years were illiterate. The majority of leprosy cases migrated within cluster 1, which includes the northern states of Pará, Piauí, Tocantins and Maranhão. More than half (56. 3%) of cases moved between municipalities in Maranhão, followed with fewer cases to neighboring Pará (11. 8%), Piauí (3. 9%) and Tocantins (2. 0%), and one-fifth of migrants were drawn to non-contiguous states. All those with leprosy migrated into a highly endemic cluster on at least one occasion, not including their current residence. Nearly one in six migrants with leprosy migrated for employment in the last five years and this was slightly less than expected for internal population movement. Typical of internal population flow, most migration in Maranhão was to urban areas (60. 3%) compared to rural areas (33. 3%), and both rural and urban areas (7. 7%). Social networks in migration destination sites for those with leprosy had a higher tendency to be family contacts with whom they lived (81. 0%) than work contacts (17. 7%). This may be an explanation for the significant number of respondents who always had a contact prior to migrating (79. 8%). Migrants with leprosy lived on average with 8. 61 people per household while migrating. Past five year migration prior to diagnosis was found to be significantly associated with leprosy as shown in Table 2 which represents the results of the multivariate logistic regression analysis. Past five year migration remained significantly associated with leprosy after controlling in separate models for 1) household contact (consanguineous and/or non-consanguineous); 2) family contact (parent, child and/or sibling; 3) and household and family contact in multiple logistic regression models. Key social, biosocial, and behavioral factors were found to be associated with leprosy (Table 3). Household, familial and other contact with someone infected with leprosy was significantly different for leprosy infected past 5-year migrants compared to control group migrants. Genetic association of closely related kinship shows a significant difference for contact with parent/child/sibling (OR: 7. 82; CI 95%: 2. 32–33. 38; P-value = 0. 0001). Contact regardless of consanguinity (OR: 4. 99; CI 95%: 1. 7–16. 51; P-value = 0. 001) and actual household contact (OR: 5. 54; CI 95%: 1. 49–30. 46; P-value = 0. 004) was also significant. An important behavioral factor distinguishing migrants with leprosy compared to the clinically unapparent control group was past five year alcohol consumption (OR: 4. 46; CI 95%: 1. 43–14. 15; P-value = 0. 005). Income and other socioeconomic variables showed significant differences between migrants with leprosy and the control group. Income less than the minimum wage (OR: 2. 12; CI 95%: 0. 97–4. 71; p-value = 0. 049) as well as poor access to public waste services (OR: 3. 1; CI 95%: 1. 1–10. 02; p-value = 0. 03) and family illiteracy (OR: 2. 67; CI 95%: 1. 13–6. 51; p-value = 0. 02) were found to be associated with leprosy among past 5-year migrants. Education, presence of BCG scar, zone of residence and lifestyle stressors - separation from family and friends, loss of employment or income, marital separation or death of close friend or relative- were not significantly associated with leprosy among past five year migrants.
  Leprosy was found to be associated with past five year migration, even after controlling for confounders. In the comparison of past 5-year migrants, leprosy was associated with both household consanguineous and/or non-consanguineous contact, close family and other social leprosy contact, consistent with research identifying contact exposure as the major determinant of leprosy transmission [14]–[15]. However, the magnitude of effect for leprosy among migrants in our study was most significant among close family and household contacts. As migration in Maranhão was largely facilitated through family networks, contact surveillance should include migration site residence contacts as well as current residence contacts. While patterns of migration, including movement in and between highly endemic clusters, were not different among migrants with leprosy and clinically unapparent migrants in the control group, important facets of migration emerged that could benefit leprosy control at the state and national level. State control programs should consider monitoring past five year residence among those newly diagnosed with leprosy to identify intra- and inter-state migration flow. This may provide early warning systems for localized disease control in areas yet to be identified as high-risk areas. Alcohol consumption in the years prior to diagnosis may be associated with susceptibility to leprosy. Alcohol consumption and consumption frequency should be included in future investigations. This research will help to determine the extent that alcohol consumption plays a role in the dynamics of both transmission and expression of leprosy. As alcohol consumption has also been associated with leprosy relapse, further attention should be given to alcohol consumption during treatment, patient relapse and contact exposure to leprosy. Other substances should also be given attention in future research. Other research in Brazil has found a spatial relationship to migration and distribution of leprosy and an association of leprosy with poor socio-economic conditions [4]–[6]. Our research shows that in endemic areas leprosy is not only associated with population movement itself, but, most importantly with the social conditions of the migrant in the endemic areas, their behavior, and contact with leprosy in the family and household.
summary: In Brazil, leprosy remains a significant public health problem in endemic clusters of high transmission risk throughout the country. Migration is thought to be a factor associated with continued leprosy transmission, as migration has also been found to be associated with other Neglected Tropical Diseases (NTDs). We analyzed the association between past five year migration and leprosy as part of a larger epidemiological study evaluating risk factors for infection among recently diagnosed leprosy cases (n = 340) and a matched clinically unapparent control group (n = 340) in the northeastern state of Maranhão. Among migrants with leprosy, 23. 5% (n = 80) migrated in the past five years, with 16. 2% (n = 55) of the control group. Past five year migration was significantly associated with leprosy, and remained significant after controlling for household and familial contact as potential confounders. Factors found to be associated with leprosy among past 5-year migrants included alcohol consumption, poverty, and household, family and other leprosy contact. Key patterns of movement emerged from the study that may aid future regional leprosy control efforts.
keywords: social epidemiology
  medicine
  infectious diseases
  socioeconomic aspects of health
  behavioral and social aspects of health
  infectious disease epidemiology
  global health
  epidemiology
  alcohol
  neglected tropical diseases
  leprosy
  public health
title: Patterns of Migration and Risks Associated with Leprosy among Migrants in Maranhão, Brazil