article: Characterization of the genetic defects causing gonadotropic deficiency has made a major contribution to elucidation of the fundamental role of Kisspeptins and Neurokinin B in puberty onset and reproduction. The absence of puberty may also reveal neurodevelopmental disorders caused by molecular defects in various cellular pathways. Investigations of these neurodevelopmental disorders may provide information about the neuronal processes controlling puberty onset and reproductive capacity. We describe here a new syndrome observed in three brothers, which involves gonadotropic axis deficiency, central hypothyroidism, peripheral demyelinating sensorimotor polyneuropathy, mental retardation, and profound hypoglycemia, progressing to nonautoimmune insulin-dependent diabetes mellitus. High-throughput sequencing revealed a homozygous in-frame deletion of 15 nucleotides in DMXL2 in all three affected patients. This homozygous deletion was associated with lower DMXL2 mRNA levels in the blood lymphocytes of the patients. DMXL2 encodes the synaptic protein rabconnectin-3α, which has been identified as a putative scaffold protein for Rab3-GAP and Rab3-GEP, two regulators of the GTPase Rab3a. We found that rabconnectin-3α was expressed in exocytosis vesicles in gonadotropin-releasing hormone (GnRH) axonal extremities in the median eminence of the hypothalamus. It was also specifically expressed in cells expressing luteinizing hormone (LH) and follicle-stimulating hormone (FSH) within the pituitary. The conditional heterozygous deletion of Dmxl2 from mouse neurons delayed puberty and resulted in very low fertility. This reproductive phenotype was associated with a lower number of GnRH neurons in the hypothalamus of adult mice. Finally, Dmxl2 knockdown in an insulin-secreting cell line showed that rabconnectin-3α controlled the constitutive and glucose-induced secretion of insulin. In conclusion, this study shows that low levels of DMXL2 expression cause a complex neurological phenotype, with abnormal glucose metabolism and gonadotropic axis deficiency due to a loss of GnRH neurons. Our findings identify rabconectin-3α as a key controller of neuronal and endocrine homeostatic processes.
  Puberty is defined by the appearance of secondary sexual characteristics and the maturation of reproductive function. It is driven by an increase in sexual steroid hormone synthesis by the gonads, under the control of the hypothalamo–pituitary hormonal axis [1]. The key event in puberty initiation is an increase in the pulsatile release of GnRH by hypothalamic neurons, triggering the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by the pituitary gland [2]. This pubertal increase in GnRH secretion is associated with increases in glutamatergic inputs and decreases in GABAergic inputs to GnRH neurons [3]. It is also facilitated by hypothalamic glial cells, which interact directly or indirectly with GnRH neurons [4]. Kisspeptins (KPs) are currently thought to be the principal hypothalamic neuropeptides controlling GnRH secretion in mammals, not only at puberty but also during adulthood [5], [6]. The activation of the KP signaling pathway in the hypothalamus is seen as the major hallmark of puberty onset [5], [6], and its control is partly epigenetic [7]. Congenital hypogonadotropic hypogonadism (CHH) is a medical condition defined by a defect of the hypothalamo–pituitary–gonadotropic (HPG) axis resulting in an absence of puberty and infertility in adulthood. Characterization of the genetic defects underlying the isolated form of CHH [8]–[12] has proved crucial, not only for elucidating the fundamental role of KPs in the central regulation of the gonadotropic axis and puberty [5], [6] but also for determining the role played by Neurokinin B (NKB) [13]. CHH may be associated with anosmia, due to olfactory bulb agenesis, in Kallmann syndrome (KS) [14], [15]. In KS, GnRH neurons fail to leave the olfactory placode (OP). Indeed, GnRH neurons, unlike other neurons of the CNS, migrate early in embryogenesis, from the OP into the hypothalamus, along the olfactory tract [16]. All genes linked to KS encode proteins necessary for olfactory bulb development or for the migration and maturation of GnRH neurons [15]. CHH has also been described in highly complex neurodevelopmental disorders caused by loss-of-function mutations in genes encoding proteins involved in diverse cellular pathways [17]–[21]. This association indicates that the same etiopathogenic mechanism may be responsible for a specific neuroendocrine deficiency and common neurological dysfunctions. We recently decided to investigate these common mechanisms further, by characterizing the molecular mechanism of a new syndrome that associates GnRH deficiency with complex neurological and endocrine phenotypes. A genetic analysis of this family revealed that this phenotype was due to a weak expression of DMXL2, encoding Rabconnectin-3α. Through in vivo and in vitro analysis of rabconnectin-3α expression and function, we discovered that weak Dmxl2 expression in the adult mouse brain resulted in a loss of GnRH neurons in the hypothalamus. These findings established a novel molecular mechanism leading to gonadotropic axis deficiency and paved the way for improvements in our understanding of the neuroendocrine control of reproduction.
  We investigated a consanguineous Senegalese family with five children, three of whom had an unusual, progressive endocrine and neurodevelopmental disorder, with similar clinical features in each case (Figure 1A and Table 1). This syndrome began early in childhood, with growth retardation and recurrent episodes of profound asymptomatic hypoglycemia (blood glucose concentration as low as 1. 6 mmol/l), with an incomplete suppression of insulin concentrations (2 to 4 mIU/l), appearing between the ages of 2 and 5 ys. Between the ages of 14 and 16 y, the three brothers gradually developed progressive nonautoimmune insulin-dependent diabetes mellitus. Their puberty was incomplete and they had a low testicular volume. All the brothers had a normal sense of smell. During adolescence, the brothers had walking difficulties, such as ataxia and dysarthria. Neurological examination showed polyneuropathy, with a motor deficit predominantly affecting the proximal lower limbs, pes cavus, and claw toes, together with cerebellar and pyramidal signs. Electrophysiological patterns were consistent with a demyelinating polyneuropathy, with a diffuse homogeneous pattern of slowing motor conduction in peripheral nerves (23–42 m/s; N>50 m/s) and greater preservation of sensory conduction in the legs than in the arms. Brain MRI showed moderate subcortical temporal white matter disease, and one patient (no. 123) also had mild hypoplasia of the cerebellum and a small anterior pituitary. The posterior lobe of the pituitary was in the correct position. Hormonal assays in adulthood showed an increase in HbA1c levels to up to 11. 7%, with low insulin concentrations in an intravenous glucose tolerance test and subnormal basal C-peptide secretion; serum-free T4 concentrations were low, with normal TSH concentrations (Table 2). The effects of TRH administration indicated that this central hypothyroidism was probably of hypothalamic origin. All three brothers had low plasma testosterone concentrations for their age, along with low LH and FSH concentrations. A GnRH test (100 µg i. v.) was performed in two patients (nos. 123 and 124 at 21 and 20 y of age, respectively). It revealed a large increase in LH concentrations associated with a normal increase in FSH concentrations (Table 2). The mother experienced menarche at the age of 13 y and her first spontaneous pregnancy occurred at 20 y. The father reported normal pubertal development. He developed glucose intolerance by the age of 46 y. Both parents were of normal height, at 160 and 175 cm. Assuming a fully penetrant recessive transmission model with a disease allele frequency of 0. 00001, two candidate regions were identified on chromosomes 13 and 15, with a LOD score of 2. 5 (Figure 1B). These two regions harbored 164 and 341 genes. High-throughput sequencing of all exons of both candidate regions in the DNA of the proband and his mother revealed an in-frame deletion of 15 nucleotides (c. 5824_5838del) in exon 24 of DMXL2, which encodes rabconnectin-3α (Rbcn-3α) (Figure 1C and Figure S1). This deletion results in the deletion of five amino-acid residues SDGNG (p. 1942_1946del) (Figure 1C). Both parents and one unaffected sister were heterozygous for the deletion; one unaffected sister did not carry the deletion (Figure S1). This pattern of familial segregation is consistent with recessive transmission. The Ser1942 residue is highly conserved between species, whereas the other four residues are not well conserved. We suspected that the 15-nucleotide deletion in DMXL2 might modify the level of expression of this gene [22]. In fact, quantitative RT-PCR showed that there was significantly less DMXL2 mRNA in homozygous c. 5824_5838del patients than in heterozygous or wild-type (WT) family members and unrelated controls (Figure 1D). These results suggested that the c. 5824_5838del probably exerted its pathogenic effects by decreasing the expression of DMXL2. We tested this hypothesis by searching for DMXL2 mutations in 10 cases of HH with a similar phenotype. No DMXL2 mutation was found in any of these patients. We therefore carried out studies in rodents, to confirm the link between the decrease in DMXL2 expression and the phenotype. Rbcn-3α is a synaptic protein that interacts closely with WDR7 [23] and may serve as a scaffold protein for both Rab3-GEP and Rab3-GAP on synaptic vesicles [23], [24]. Rbcn-3α has also been implicated in the regulation of Notch signaling, through the modulation of v-ATPAse activity in mammalian and insect cells [25]. We therefore analyzed the expression of Dmxl2 and Rbcn-3α in both the brain and pituitary in rodents. Both Dmxl2 mRNA and Rbcn-3α were detected in the hippocampus, dentate gyrus, hypothalamus, pyriform cortex (Figure 2A–E), and the granular and molecular layers of the cerebellum of adult mice (Figure S2). In the hypothalamus, immunostaining for Rbcn-3α was observed in the arcuate nucleus (ARC), the median eminence (ME), the organum vasculosum of the lamina terminalis (OVLT), (Figure 2C, D), and the subfornical organ (SFO), the subcommissural organ, and the suprachiasmatic nucleus (SCN) (Figure S2). The external layer of the ME displayed a punctate pattern of immunostaining for Rbcn-3α (Figure 2E). Staining was also observed in the cell bodies lining the third ventricle and in the long processes extending from these cell bodies toward the external layer of the ME (Figure 2E). Rbcn-3α was detected in small clear vesicles (Figure 2F) and in large dense core vesicles (LDCVs) (Figure 2G). This pattern of staining strongly suggests that both tanycytes and hypothalamic neurosecretory neurons express Rbcn-3α. In the anterior pituitary of adult rats, Rbcn-3α immunostaining was observed only in cells producing LH and FSH (Figure 3A), the pattern observed being similar to that for Rab3-GEP and Rab3-GAP (Figure 3B). None of these three proteins colocalized with ACTH, TSH, or GH (Figure 3C). We then investigated whether a decrease in Dmxl2 expression in mice could reproduce the phenotype observed in the human patients. For this purpose, we used a targeted gene-trapping vector to delete Dmxl2 in mice [26]. In this strategy, the recombinant allele (tm1a) contained an IRES: LacZ trapping cassette and a floxed promoter-driven neo cassette in intron 6 and two loxP sites, one at either end of exon 7 of Dmxl2 (Figure S3). The tm1a allele encodes a truncated form of Rbcn-3α. Dmxl2-heterozygous hypomorphic mice (Dmxl2tm1a/wt) displayed no obvious morphological, growth deficit (Table S1). Dmxl2tm1a/wt mice were fertile. A tendency for a slightly later vaginal opening (VO), an important external indicator of puberty onset, and a longer time between VO and first estrus, were observed in Dmxl2tm1a/wt mice, but these differences were not significant (Figure S4). Although fertile, these mice produced no homozygous offspring (Dmxl2tm1a/tm1a) when crossed (Table S2). As the gene-trapping method may generate a hypomorphic allele [27], [28] and we expected the severity of the phenotype to be correlated with Dmxl2 expression levels in neurons, we investigated whether a mouse line with a complete KO of Dmxl2 in neurons had a more severe phenotype. We crossed Dmxl2tm1a/wt mice with WT mice expressing the FLIP (flp) recombinase, which recognizes the FLP recombinase target (FRT) sequence flanking the β-galactosidase-neomycin cassette in the tm1a allele. This led to the production of mice bearing two WT alleles, with two loxP sites flanking exon 7 of Dmxl2 (Dmxl2lox/lox). These Dmxl2lox/lox mice were then crossed with mice expressing the Cre-recombinase under the control of the Nestin promoter (nes-Cre; Dmxl2wt/wt) [29], to obtain mice bearing a Dmxl2 allele from which exon 7 was deleted in nestin-expressing cells (nes-Cre; Dmxl2–/wt) (Figure S3). Adult nes-Cre; Dmxl2–/wt mice displayed no obvious morphological defects. Hypothalamic Dmxl2 mRNA levels were 50% lower in nes-Cre; Dmxl2–/wt than in Dmxl2lox/wt mice (Figure S3). Female nes-Cre; Dmxl2–/wt mice weighed less than WT mice (Figure 4A), whereas weight differences between males of these two genotypes were only observed from PND 30 to PND 40 (Figure S5). We first analyzed reproductive function. VO and first estrus occurred significantly later in female nes-Cre; Dmxl2–/wt mice than in their WT littermates (Figure 4B and 4C). The time from VO to first estrus was significantly longer in nes-Cre; Dmxl2–/wt mice (Figure 4D). Very few nes-Cre; Dmxl2–/wt females completed a full estrous cycle over a 20-d period (Figure 4E), and they spent less time in high estradiol levels (Figure 4F). In nes-Cre; Dmxl2–/wt males, the anogenital distance (AGD) was significantly shorter than that in their WT littermates, providing an external indicator of low testosterone concentration (Figure 4G). Fertility was assessed over a period of 3 mo. The female nes-Cre; Dmxl2–/wt mice had very poor fertility, producing few, if any litters, and the nes-Cre; Dmxl2–/wt males were subfertile (Table S3). Phenotypic analyses were then pursued to characterize the fertility problems observed in nes-Cre; Dmxl2–/wt mice. The weights of testes and ovaries were low in nes-Cre; Dmxl2–/wt mice (Figure 5A and 5B). A histological analysis of ovaries in female nes-Cre; Dmxl2–/wt mice revealed the presence of normal numbers of antral follicles associated with significantly fewer corpora lutea than would normally be expected (Figure 5C). Estradiol concentrations were normal in nes-Cre; Dmxl2–/wt females (Figure 5D), whereas testosterone concentrations were significantly lower in nes-Cre; Dmxl2–/wt males than in their WT littermates (Figure 5E). LH concentrations were moderately, but significantly, higher in nes-Cre; Dmxl2–/wt females than in WT females (Figure 5F), whereas no difference in LH concentration was observed between nes-Cre; Dmxl2–/wt and WT males (Figure 5G). The normal concentrations of LH observed despite the low concentration of testosterone in male nes-Cre; Dmxl2–/wt mice was indicative of a partial CHH due to a hypothalamic defect. Consistent with this, nes-Cre; Dmxl2–/wt mice displayed normal increases in LH concentration following the intraperitoneal injection of GnRH (Figure 5H). The normal response to PMSG injection of ovaries from nes-Cre; Dmxl2–/wt mice confirmed that the very low rate of ovulation in nes-Cre; Dmxl2–/wt females was related to a defect in the hypothalamo–pituitary control of the gonadotropic axis (Figure 5I). The delayed puberty and infertility observed in nes-Cre; Dmxl2–/wt mice was therefore due largely to a hypothalamic defect. We characterized the hypothalamic defect in nes-Cre; Dmxl2–/wt mice, by determining whether Rbcn-3α was produced in GnRH neurons. Punctate Rbcn-3α staining was colocalized with GnRH in the median and external layers of the ME (Figure 6A). Electron microscopy confirmed that Rbcn-3α was present in GnRH neurons (Figure 6B). We investigated whether the gonadotropin deficiency observed in nes-Cre; Dmxl2–/wt mice was due to GnRH deficiency, by quantifying GnRH mRNA levels in the hypothalamus of nes-Cre; Dmxl2–/wt mice and comparing them with those of WT mice. GnRH mRNA levels were significantly lower in nes-Cre; Dmxl2–/wt mice than in WT mice (Figure 6C). This decrease was specific to GnRH, as the amounts of mRNA for other hypothalamic-releasing hormones were normal (Figure S6). The lower levels of GnRH mRNA were associated with the presence of fewer GnRH-ir neurons in the hypothalamus of nes-Cre; Dmxl2–/wt mice than in WT littermates (Figure 6D). An analysis of the rostral–caudal distribution of GnRH-ir neurons from the diagonal band of Broca to the ME showed that there were significantly fewer GnRH-ir neurons in the OVLT, this loss of neurons not being compensated by an increase in the number of GnRH-immunoreactive (GnRH-ir) neurons in the rostral or caudal parts of the hypothalamus (Figure 6E and 6F). The poor fertility observed in nes-Cre; Dmxl2–/wt mice was therefore associated with the presence of fewer GnRH-ir neurons in the hypothalamus. As patients with the c. 5824_5838del of DMXL2 displayed ataxia and mental disability, we investigated whether Dmxl2lox/wt or nes-Cre; Dmxl2–/wt mice displayed neurological defects. We analyzed the gait and exploratory behavior of these mice in an open-field system [30]. No significant difference of the gait between Dmxl2tm1a/wt mice and their WT littermates was observed. However, nes-Cre; Dmxl2–/wt mice displayed significantly higher levels of exploratory behavior than their WT littermates (Figure S7). Severe abnormal glucoregulation, consistent with impaired insulin secretion, was observed in the three affected brothers. Rbcn-3α forms a complex with regulators of Rab3 GTPase, and this complex is involved in the docking and priming of exocytosis vesicles [31]. Insulin-secreting pancreatic islet β cells express many proteins involved in the exocytosis of synaptic vesicles [32], [33]. In fact, Rbcn-3α was found to be present in pancreatic β cells (Figure 7A). We therefore hypothesized that Rbcn-3α might be involved in the regulated secretion of insulin. We tested this hypothesis, by using siRNA to down-regulate Dmxl2 expression in an insulin-secreting cell line (INS-1E). We observed a significant decrease in Dmxl2 mRNA levels, to 25% those in nontarget (NT) siRNA-transfected cells, 72 h after the transfection of INS-1E cells with the Dmxl2 siRNA (Figure 7B). The knockdown of Dmxl2 expression with Dmxl2 siRNA resulted in significantly higher levels of basal insulin secretion than for NT siRNA-transfected cells (Figure 7C). By contrast to what was observed for NT siRNA-transfected cells, the incubation of Dmxl2 siRNA-transfected cells with 5 mM glucose did not increase insulin secretion, and only a slight increase in insulin secretion was observed after incubation with 20 mM glucose (Figure 7C). These in vitro experiments demonstrate the involvement of Rbcn-3α in the control of regulated insulin secretion.
  In this study, we found that a low level of DMXL2 haploinsufficiency caused a previously unknown complex phenotype in humans. The Warburg Micro and Martsolf syndromes were considered the most relevant syndromes for comparison with the phenotype reported here, as these syndromes are caused by mutations affecting the catalytic (RAB3GAP1, p130) and noncatalytic subunits (RAB3GAP2, p150) of RAB3GAP [18], [19], two components of the protein complex containing Rbcn-3α. Hypogonadotropic hypogonadism, mental retardation, and demyelinating peripheral neuropathy have been reported in patients with RAB3GAP1 or RAB3GAP2 mutations [34], but the bilateral cataracts, microphthalmia, and microcornea systematically found in patients with the Warburg Micro and Martsolf syndromes were not observed in this family. Furthermore, insulin-dependent diabetes mellitus has not been reported in these syndromes. The phenotype observed in this family was therefore different from those of the Warburg Micro and Martsolf syndromes. Mice expressing a conditional heterozygous null allele of Dmlx2 in neurons have very low fertility. This finding contrasts with the observed phenotype of mice expressing a hypomorphic Dmxl2 allele and with findings for the family studied here, in which the two c. 5824_5838del-heterozygous parents were clearly fertile. We suspect that the severity of the phenotype is highly associated to the neuronal expression level of Dmxl2. The level of Rbcn-3α expression in the neurons of nes-Cre; Dmxl2–/wt mice was found to be 50% that in WT animals, whereas Dmxl2 expression in the neurons of Dmxl2tm1a/wt mice was thought to be more than 50% that observed in WT mice. DMXL2 mRNA levels were about 30% normal values in subjects homozygous for c. 5824_5838del, whereas they were about 60% normal values in subjects heterozygous for this deletion (see Figure 1). Furthermore, we were unable to obtain viable Dmxl2tm1a/tm1a mice. These mice died during embryonic development, indicating a requirement of Rbcn-3α for normal development in mice. This correlation between DMXL2 expression level and the severity of the neurological and neuroendocrinological phenotypes in both mice and humans is consistent with a probable synaptic function for Rbcn-3α. The phenotypic discrepancy between mice and humans may also result from the interactions of different partners with Rbcn-3α. It may also reflect the fact that the threshold of the neuronal expression level of Dmxl2 causing a phenotype may be species-specific. An investigation of gonadotropic axis function in adult nes-Cre; Dmxl2–/wt mice showed that the partial gonadotropic deficiency was associated with the presence of a smaller total number of GnRH-ir neurons. Multiple mechanisms may be involved, such as a defect of GnRH neuron differentiation in the OP, migratory failure, or a defect in the maturation and postnatal survival of these GnRH neurons. The latter follow a unique migratory route, emerging in the OP at E10. 5 and then entering the forebrain at E. 13. 5, to migrate along olfactory/vomeronasal nerve axons before turning toward the hypothalamus, where they are dispersed in the preoptic area (POA) and extend their axons by E16. 5 [35]. Many of the factors controlling GnRH neuron migration also influence or control the development of the olfactory system [35]. Olfactory bulbs developed normally in nes-Cre; Dmxl2–/wt mice, demonstrating that the smaller number of GnRH neurons in the POA region of nes-Cre; Dmxl2–/wt adult mice was not associated with a defect of olfactory/vomeronasal nerve axon development. This result is consistent with the absence of anosmia and olfactory bulb agenesis in the patients with DMXL2 mutations. Furthermore, the analysis in adult mice showed that the migration of GnRH neurons was not halted in the forebrain. Preliminary data suggested that adult nes-Cre; Dmxl2–/wt mice had a GnRH neuron morphology in the rostral POA that was more complex than the normal unipolar/bipolar morphology displayed by their WT littermates. This region contains the GnRH neuron population of the greatest importance for the onset of puberty and fertility. This complex morphology may indicate that GnRH neurons did not progress toward a mature form. This led us to suspect that there might be a defect in the postnatal development of GnRH neurons in nes-Cre; Dmxl2–/wt mice. It is interesting to note that Rbcn-3α was recently shown to be required for neural crest cell migration and cell fate determination by controlling the Wnt signaling pathway [36]. The observation that siRNA-induced down-regulation of Dmxl2 expression led to an increase in spontaneous insulin release and a disruption of glucose-induced insulin release in INS-1E cells is consistent with a role for Rbcn-3α in controlling the secretion of insulin in vivo. The episodes of hypoglycemia during childhood in the human patients, associated with the lack of a blockade of insulin release, may be accounted by a decrease in the inhibition of basal insulin release by Rbcn-3α. By contrast, the occurrence of insulin-dependent diabetes mellitus during adolescence in the affected patients indicates an inability of the pancreas to respond to the increase in insulin requirement at puberty [37]. This role of Rbcn-3α in controlling insulin release is consistent with the recent report of an association between the MAP kinase-activating death domain (MADD) protein locus, also known as RAB3GEP, with fasting glycemia and plasma proinsulin concentrations [38], [39]. Rab27a is a monomeric GTPase protein very similar to Rab3. Rab27a mediates glucose-specific signals for the exocytosis of insulin vesicles in the pancreas [40]. Further investigations are required to confirm the role of Rbcn-3α in insulin release in vivo and to determine whether this protein may also act via the Rab27 pathway. As the cellular machinery controlling vesicle exocytosis is similar in pancreatic β cells and neurons [32], [33], we can assume that Rbcn-3α is involved in neurosecretion. Rbcn-3α may exert its neurosecretory functions by controlling the “on/off” activity of Rab3a [41], [42]. However, the severity of the phenotypes observed in this family and in patients with RAB3GAP1 and RAB3GAP2 mutations [18], [19] contrast with the mild deficiency of Ca2+-triggered neurotransmitter release caused by Rab3a deletion [43]. Other vesicle exocytosis steps may thus be impaired when Dmxl2 expression levels are low in neurons [44]–[46]. The neurotransmitter glutamate is involved in the regulation of GnRH secretion [3]. Glutamate release is controlled by the amount of active Rab3a [42], suggesting that glutamate-induced GnRH neuron activation may be impaired in nes-Cre; Dmxl2–/wt mice. This possible dysfunction of the glutamatergic input in GnRH neurons may be responsible for the loss of GnRH neurons in nes-Cre; Dmxl2–/wt mice. Defective V-ATPase–mediated acidification of exocytosis vesicles may also contribute to the phenotype [47]. The expression of Rbcn-3α, Rab3-GEP, and Rab3-GAP in gonadotropes only suggests that these three proteins may specifically regulate the secretion of LH and FSH. A dual defect in hypothalamic neuropeptide and gonadotropin secretion may thus contribute to the gonadotropic axis deficiency observed in the studied family. We found in this study that low levels of Dmxl2 expression caused a complex neurological and endocrine phenotype in humans. Low levels of Dmxl2 expression in neuronal cells of mice lead to a partial gonadotropic axis deficiency, resulting in a very low fertility, due to a loss of GnRH neurons in the hypothalamus. Our results strongly suggest that the loss of these neurons is due to a dysfunction of peptide/neurotransmitter release by GnRH neurons itself or the neurons innervating GnRH neurons. This association of a low level of expression of a vesicular protein and the loss of GnRH neurons constitutes a new mechanism of GnRH deficiency. This study opens up new possibilities for characterizing the pathogenic mechanisms of GnRH deficiency and the neuronal control of the GnRH network.
  DNA was extracted from peripheral blood lymphocytes and genotyped with an Affymetrix 250k Nsp array, according to the manufacturer' s instructions. Linkage analysis was performed by classical techniques [48], [49]. Call rates were >98%, and multipoint LOD scores were calculated with Allegro-v1. 2c [49], after the random selection of one informative SNP every 500 kb, following data quality control with Alohomora-v0. 33 software [48]. A fully penetrant recessive transmission model was assumed, with a disease allele frequency of 0. 00001. Two genomic regions were found to have LOD scores of 2. 5, corresponding to the maximum possible LOD score for this family. High-throughput sequencing of the two candidate regions was performed at the “Centre National de Génotypage” in Evry, France. Sanger sequencing of DMXL2 exons was performed for all family members (Figure S1) and for 10 additional patients. Primer sequences are available upon request. Total RNA was extracted from blood lymphocytes from the patients, with a QiaAMP RNA Blood Mini Kit (Qiagen), according to the manufacturer' s instructions. Total RNA was extracted from mouse tissues with Trizol (Life Technologies). The first-strand cDNA was synthesized with the Superscript II first-strand synthesis kit (Invitrogen), with random primers or oligodT. Human DMXL2 and 18S mRNA levels in the patients' lymphocytes were quantified in specific DMXL2 and RNA 18S Taqman gene expression assays on a 7300 Real-Time PCR System (Applied Biosystems). Reverse transcription–quantitative PCR (RT-qPCR) analysis of gene expression in INS-1E cells or in mouse tissues was performed with the SyberGreen MasterMix (BioRad, Hercules, CA), in a CFX 384 Real-Time PCR Detection System (BioRad). The primer sequences used are available upon request. Gene expression levels are expressed relative to those of RNA 18S or GAPDH, as follows: 100×2 (e−ΔCtGene/e−ΔCtHousekeeping Gene). We analyzed Dmxl2 expression in the brain by performing ISH on C57Bl6 mouse brain, with a probe generated from a DNA template obtained by PCR. A digoxigenin UTP-labeled Dmxl2 probe was synthesized by generating a DNA template by PCR, with the Expand High-Fidelity PCR system (Roche Diagnostics) and a pair of primers binding between nucleotides 222 and 1068 of the mouse Dmxl2 cDNA sequence (NCBI Reference Sequence NM_172771. 2) and carrying the SP6 and T7 promoter sequences. The PCR product was used as the DNA template for the transcription of digoxigenin-UTP probes with an SP6 and T7 DIG RNA labeling kit (Roche Diagnostics). ISH was carried out on 200-µm mouse brain sections. Mouse brains were embedded in a 5% agarose/BSA preparation and sectioned with a vibratome (Leica VT 1000 S). Sections were dehydrated by passage through a graded series of ethanol solutions and were stored frozen at −20°C. Sections were rehydrated and ISH was carried out as previously described [50]. Sections were mounted on glass slides, in 80% glycerol in PBS, and images were captured under a microscope (Leica DM IRB), at a magnification of 1. 6×. Half-brain images were reconstituted from several images of the same section, taken at the same time and under the same conditions. For the analysis of Rbcn-3α expression in the rodent pituitary and brain, tissues were prepared from C57BL6 mice and/or WKY rats, as previously described [51]. The rodents were deeply anesthetized by pentobarbital injections and perfused transcardially with 4% paraformaldehyde in 0. 1 M phosphate-buffered saline (PBS), pH 7. 4. Brains were removed, cryoprotected in 30% sucrose, frozen in liquid isopentane at −45°C, and stored at −80°C until sectioning. We cut 30-µm coronal sections on a cryostat, and the free-floating sections were processed for IHC. For immunofluorescence, sections were incubated with 5% normal donkey serum (NDS) and 0. 4% Triton X-100 in PBS (PBS-T) for 45 min. Sections were then incubated with primary antibodies in 1% donkey serum in PBS-T, for 48 h at 4°C (Table S4). Sections were rinsed several times with PBS and then incubated with a fluorescent secondary antibody for 1 h at room temperature (RT). They were then washed three more times in PBS, counterstained with TO-PRO-3 (1/500, Invitrogen), rinsed in PBS, mounted on Superfrost Plus slides (Microm Microtech) in Fluoromount (Southern Biotech), and covered with a coverslip. For immunoperoxidase staining, the same procedure was followed, except that the sections were incubated with a biotinylated secondary antibody for 90 min at RT. The sections were incubated with 1% H2O2 in PBS for 10 min, rinsed in PBS-T, and labeling was detected with the Vectastain ABC kit (Vector Laboratories, Burlingame). Sections were then mounted in Pertex resin. The analysis of the GnRH neuron distribution was processed with the SW-1 antibody [52] at a dilution of 1∶3,000 in PBST (0. 4%), for 48 h at 4°C, in the presence of 2% NDS and then as described above. The total number of GnRH-IR neurons was quantified in serial 40-µm coronal sections from the anterior preoptic region to the posterior hypothalamus in adult mice (PND 60), as previously described [53]. All slides were coded and scored blind. A neuron was scored as GnRH-IR only if it had a clearly stained cell body. We analyzed the distribution of GnRH-ir neurons further, designating the start of the OVLT as section 0. We numbered the 20 sections on either side of section 0 in the OVLT. This analysis did not include the regions in the immediate vicinity of the olfactory bulbs, but it did encompass most of the GnRH neuronal system of the mouse brain. For double-labeling fluorescent IHC, sections were first rinsed in PBS and preincubated for 30 min in 5% NDS in PBS containing 0. 3% Triton X-100. Sections were coincubated overnight at RT in ADD AbIs diluted in PBS containing 1% NDS and 0. 3% Triton X-100. Sections were then rinsed in PBS and incubated for 1 h in GIVE THE AbIIs Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1∶200; Molecular Probes) and Cy3-conjugated donkey anti-mouse IgG (1∶300; Jackson ImmunoResearch) diluted in PBS containing 3% NDS and 0. 3% Triton X-100. Sections were then rinsed in PBS, counterstained with DAPI (1/4,000), mounted on glass slides, and coverslipped with Fluoromount-G solution (Southern Biotech) for confocal microscopic examination. Immunofluorescent sections were analyzed using a Leica TCS SP8 confocal scanning system (Leica Microsystems) equipped with 488-nm Ar, 561-nm DPSS, and 633-nm HeNe lasers. Eight bit digital images were collected from a single optical plane using a 63× HC PL APO CS2 oil-immersion Leica objective (numerical aperture, 1. 40) or a 40× HC PL APO CS2 oil-immersion Leica objective (numerical aperture, 1. 30). For each optical section, double/triple-labelled images were acquired in sequential mode to avoid potential contamination by linkage-specific fluorescence emission cross-talk. C57BL6 WT adult mice (PND 60) were anesthetized with 0. 2 cm3 of 10% chloral hydrate and intracardially perfused with 100 ml of 4% PFA, 0. 1% glutaraldehyde in 0. 1 M phosphate buffer (pH 7. 4) on ice. Brains were postfixed by overnight incubation in 4% PFA at 4°C. The following day, the hypothalamus was dissected and cut into 200-µm sections in ice-cold PBS. These sections were then processed for pre-embedding IHC with an antibody against rabconnectin-3 [54]. For this purpose, sections were immersed for 20 min in 50 mM ammonium chloride in PBS, rinsed, and incubated overnight at 4°C with rabbit anti–rabconnectin-3α antibody (1∶500) in PBS containing 0. 1% gelatin (PBSg). Antibody binding was detected by incubating sections with a goat anti-rabbit biotinylated antibody (1∶200) in PBS-gelatin for 4 h at RT and then with an avidin–peroxidase complex (1∶200) for 3 h (Elite Vectastain kit, Vector Laboratories). Finally, sections were incubated with 0. 6% diaminobenzidine in Tris-buffered saline and then with Sigma Fast DAB. After staining, the region of interest, the ME, was further dissected and embedded in epoxy resin, for the cutting of semithin sections (0. 5 µm), which were stained for visualization of the ME. Ultrathin sections (70 nm) of the ME were then cut and collected on 400-mesh formvar-coated nickel grids and examined with a Jeol JEM-100CXII electron microscope. For dual labeling with Rbcn-3α and GnRH antibodies, the ME was carefully dissected out, rinsed in PBS, and incubated in 20% glycerol/20% sucrose in PBS for 3 h. ME sections were then placed carefully in liquid nitrogen for three freeze cycles, rinsed in NH4Cl, and washed in PBSg. ME sections were incubated overnight in mouse anti-GnRH antibody, at a dilution of 1∶200 in PBSg, at 4°C. The sections were then washed in PBSg, incubated with goat anti-mouse secondary antibody conjugated to gold particles (Aurion, Biovalley), at a dilution of 1∶100, for 4 h at RT (nanogold). Sections were washed in PBS. The nanogold particle signal was amplified with a BBI Amplification silver kit, for 7 min at RT, followed by gold toning. The sections were rinsed in PBS and incubated overnight at 4°C with rabbit anti–Rbcn-3α, at a dilution of 1∶500 in PBSg. Rbcn-3α staining was detected by incubating the sections with donkey anti-rabbit biotinylated antibody at a dilution of 1∶200 in PBSg at RT, rinsing in PBS, and then incubating with an avidin–biotin complex at a dilution of 1∶200, with the detection of DAB staining after postfixation (1% glutaraldehyde) with SigmaFast tablets (Sigma Aldrich). The DAB reaction was stopped with Tris-HCL, and the sections were washed in PBS. Sections were incubated with osmium tetraoxide for 1 h at 4°C and dehydrated for embedding in epoxy resin, flat embedding, and polymerization. Sections embedded in epoxy resin blocks were subsequently cut into semithin (0. 5–2 µm) and ultrathin (70 nm) sections and analyzed by electron microscopy. The role of Rbcn-3α in regulated insulin secretion was analyzed in the rat insulinoma-derived INS-1E cell line by knocking down Dmxl2 expression with siRNAs directed against Dmxl2. Glucose-induced insulin release into the supernatant of Dmxl2 siRNA-transfected cells was then compared with that from NT siRNA-transfected cells. The rat insulinoma-derived INS-1E cell line was kindly provided by Prof. Pierre Maechler and Prof. Claes Wollheim [55]. Cells were cultured in RPMI-1640 medium supplemented with 5% fetal calf serum. Twenty-four hours before transfection, cells were used to seed 96-well culture plates (BD Biosciences), in complete culture medium. They were then transfected with 100 nM siGENOME Smart Pool Rat LOC315676 siRNA against rat Dmxl2 (Thermo Scientific) or 100 nM control siGENOME NT siRNA #1 (Thermo Scientific), in the presence of Dharmafect 2 transfection reagent (Thermo Scientific). The cells were incubated for 72 h, washed once in PBS, and used for total RNA extraction with Trizol reagent. Cells transfected with either Dmxl2 or NT siRNA were incubated for 3 h with Krebs-Ringer bicarbonate buffer supplemented with 0. 35% BSA (KRBH-BSA; Sigma Aldrich). They were then incubated in the same buffer with 0,5, or 20 mM D (+) glucose monohydrate (Sigma Aldrich) for 90 min at 37°C, and insulin secretion was evaluated with the sensitive-range protocol of the HTRF insulin assay (Cisbio Bioassays). The gene targeting vector used to decrease the expression of Dmxl2 in all cells included an additional acceptor splice site in intron 6, LacZ under the control of the endogenous Dmxl2 promoter, a neomycin resistance (neoR) cassette under the control of the actin promoter, and loxP sites flanking the critical Dmxl2 exon 7 (Figure S2). The vector (Dmxl2tm1a (EUCOMM) Wtsi) was injected into JM8-F6 embryonic stem cells at the European Conditional Mouse Mutagenesis Program (EUCOMM). These cells were then injected into C57Bl/6N blastocysts at the Transgenic Animal Service (SEAT; Villejuif, France) to create chimeric mice, which were crossed to create Dmxl2 hypomorphic (Dmxl2tm1a/wt) mice. This mouse line was transferred to the INSERM U1141 animal facility, where the animals were housed under a 12 h light/12 h dark cycle and fed ad libitum. Dmxl2tm1a/wt mice were intercrossed to obtain Dmxl2wt/wt and Dmxl2tm1a/wt mice. However, we were unable to obtain any Dmxl2tm1a/tm1a mice due to embryonic lethality. A conditional KO of Dmxl2 was obtained by crossing Dmxl2tm1a/wt mice with FLIP (FLP) recombinase-expressing mice, to remove the β-galactosidase cassette and the neomycin resistance gene and to create Dmxl2lox/lox mice, which were then crossed with nes-Cre mice to obtain mice with a conditional deletion of Dmxl2 in neurons and glial cells (nes-Cre; Dmxl2–/wt). Genomic DNA was obtained on postnatal day (PND) 21 (tail biopsy) and PND 60 (brain tissue), and each animal was genotyped by PCR. For detection of the Dmxl2 GT cassette, two primers flanking the 5′ and 3′ regions of the GT cassette (Dmxl2 5′-arm and Dmxl2-3′arm) were used, together with another primer designed to bind within the 5′-arm of the GT cassette (Dmxl2 LAR3) (www. knockoutmouse. org/about/eucomm). A separate PCR analysis of genomic DNA was used to investigate the presence of the Nestin-Cre allele. The reproductive phenotype was assessed at 16: 00 hours, at 5-d intervals, in male mice and for 20 consecutive days after weaning (PND 21) in females. In males, AGD was recorded from weaning (PND 21) to PND 60 (AGD). In females, three parameters were assessed: (1) age at VO, (2) age at first estrus, and (3) the interval between VO and first estrus. Estrous cyclicity was monitored daily after VO, at 16: 00 hours, for 20 consecutive days, by vaginal lavage. Male mice underwent subcutaneous injections of 100,200,300, and 400 ng/kg GnRH in an initial trial experiment, to determine an appropriate submaximal dose for the LH response. A dose of 300 ng/kg was chosen as the submaximal dose for use in subsequent experiments. Five male mice of each genotype received 100 µl i. p. injections of 300 ng/kg GnRH (Sigma Aldrich) or vehicle (0. 9% saline). Ten minutes after injection, mice were anesthetized rapidly by isoflurane inhalation and decapitated for trunk blood collection. Plasma was isolated at 4°C, after the centrifugation of blood samples at 3,000×g for 10 min. Plasma samples were then stored at −20°C until use. Five young (PND21-PND23) female mice of each genotype underwent i. p. injections of 0. 4 IU/g PMSG (Sigma Aldrich) or vehicle (0. 9% saline). They were anesthetized with isoflurane vapor 48 h later and decapitated for trunk blood isolation. Plasma was isolated at 4°C, by centrifugation at 3,000×g for 10 min. Trunk blood from adult PND60 mice was collected into heparin, after decapitation at 16: 00. The blood was immediately placed on ice and was then centrifuged at 4°C and 3,000×g for 10 min. Plasma was subsequently harvested and stored at −20°C until analysis. Plasma luteinizing hormone concentrations were assessed by a radioimmunoassay (RIA) [56]. Plasma estradiol concentrations were determined with an estradiol enzyme immunoassay (EIA) kit (Caymen Chemicals, 582251). The 80% B/B0 detection limit for this kit is approximately 19 pg/ml, with a sensitivity of 129 pg/ml. Testosterone concentrations in humans and mice were assessed by RIA. Samples were analyzed by one-way ANOVA, with Bonferroni correction if there were more than two groups, and two-tailed Student' s t tests were used for pairwise comparisons (WT versus KO). Statistical analyses were carried out with Prism software (GraphPad, La Jolla, CA). Differences were considered significant if p<0. 05.
summary: Investigation of neurodevelopmental disorders with abnormal puberty can reveal neuronal processes that control the initiation of puberty and subsequent reproductive function. We describe here a new syndrome observed in three brothers with incomplete puberty, central hypothyroidism, peripheral polyneuropathy, mental retardation, and abnormal glucose regulation. Molecular genetic investigation in this consanguineous family revealed an in-frame deletion of 15 nucleotides in the DMXL2 gene, which resulted in lower levels of its expression. We found that rabconnectin-3α, the synaptic protein encoded by DMXL2, is widely expressed in the brain and in the ends of the axons of neurons that produce gonadotropin-releasing hormone (GnRH). We then observed that neuron-specific deletion of one allele of Dmxl2 in mice reproduces the incomplete puberty seen in the human patients and results in decreased numbers of GnRH neurons in the hypothalamus. We also showed that rabconnectin-3α controls glucose-induced insulin secretion. These findings reveal a new mechanism of gonadotropin deficiency and identify rabconnectin-3α as a key controller of neuronal and endocrine homeostasis.
keywords: biochemistry
  medicine and health sciences
  neurochemistry
  endocrinology
  biology and life sciences
  neuroendocrinology
title: Haploinsufficiency of Dmxl2, Encoding a Synaptic Protein, Causes Infertility Associated with a Loss of GnRH Neurons in Mouse