article: Proteins associated with familial neurodegenerative disease often aggregate in patients’ neurons. Several such proteins, e. g. TDP-43, aggregate and are toxic when expressed in yeast. Deletion of the ATXN2 ortholog, PBP1, reduces yeast TDP-43 toxicity, which led to identification of ATXN2 as an amyotrophic lateral sclerosis (ALS) risk factor and therapeutic target. Likewise, new yeast neurodegenerative disease models could facilitate identification of other risk factors and targets. Mutations in SS18L1, encoding the calcium-responsive transactivator (CREST) chromatin-remodeling protein, are associated with ALS. We show that CREST is toxic in yeast and forms nuclear and occasionally cytoplasmic foci that stain with Thioflavin-T, a dye indicative of amyloid-like protein. Like the yeast chromatin-remodeling factor SWI1, CREST inhibits silencing of FLO genes. Toxicity of CREST is enhanced by the [PIN+] prion and reduced by deletion of the HSP104 chaperone required for the propagation of many yeast prions. Likewise, deletion of PBP1 reduced CREST toxicity and aggregation. In accord with the yeast data, we show that the Drosophila ortholog of human ATXN2, dAtx2, is a potent enhancer of CREST toxicity. Downregulation of dAtx2 in flies overexpressing CREST in retinal ganglion cells was sufficient to largely rescue the severe degenerative phenotype induced by human CREST. Overexpression caused considerable co-localization of CREST and PBP1/ATXN2 in cytoplasmic foci in both yeast and mammalian cells. Thus, co-aggregation of CREST and PBP1/ATXN2 may serve as one of the mechanisms of PBP1/ATXN2-mediated toxicity. These results extend the spectrum of ALS associated proteins whose toxicity is regulated by PBP1/ATXN2, suggesting that therapies targeting ATXN2 may be effective for a wide range of neurodegenerative diseases.
  Mutations in an increasing number of human genes have been found to cause familial neurodegenerative disease [1,2]. Proteins encoded by these genes are often soluble in healthy individuals, but form insoluble amyloid-like aggregates that seed further aggregation in the neurons of patients with disease. For example, such conformational changes have been seen for: Aβ, associated with Alzheimer’s disease; α-synuclein with Parkinson’s disease; TDP-43, FUS and others with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD); and huntingtin with Huntington’s disease. Wild-type and mutant forms of these proteins are respectively associated with aggregates in sporadic and familial forms of the diseases. Overexpression of either wild-type or mutant protein causes toxicity, although the mutant forms are often toxic at lower concentrations than the wild-type [3]. Conformational change of several yeast proteins from a soluble state to insoluble self-seeding aggregates, called prions, causes transmissible phenotypic changes [4–15]. Furthermore, the presence of one prion aggregate enhances the de novo appearance of heterologous prions. For example, the endogenous yeast prion [PIN+] (sometimes called [RNQ+]), which is an amyloid form of the RNQ1 protein, promotes the de novo aggregation of the SUP35 protein to form the [PSI+] prion. This could occur by cross-seeding or by sequestration of proteins such as chaperones by the amyloid [PIN+] prion [8,16–27]. Yeast has proved to be useful in the study of disease-specific proteins that form prion-like aggregates [28–35]. When human proteins associated with aggregation in neurodegenerative disease were expressed in yeast, they formed aggregates and caused toxicity. Curiously the toxicity of several of these proteins, e. g. TDP-43, FUS or huntingtin, is enhanced by the presence of the [PIN+] prion [28,36,37]. These yeast models have allowed the identification of yeast genes that either alter the disease protein’s aggregation or enhance or reduce its toxicity. Remarkably, human homologs of these yeast modifier genes have confirmed, and identified new human risk factors for Alzheimer’s (PICALM, XPO1, ADSSL1 and RABGEF1) [38–40], Parkinson’s (PARK9) [38,41]) and ALS (ATXN2) [42–46]. Indeed, the discovery in yeast that deletion of the ATXN2 (Ataxin-2) ortholog, PBP1, reduced TDP-43 toxicity, led to the recent exciting finding that reduction in ATXN2 levels dramatically lowers neurotoxicity in ALS mouse models [43]. Thus the establishment of new yeast neurodegenerative disease models may lead to the identification of new risk factors for human disease as well as provide screening platforms for drug discovery. Recently, mutations in SS18L1 have been shown to cause ALS [47,48]. SS18L1 encodes the calcium-responsive transactivator (CREST) protein which is an essential component of the nBAF neuron-specific chromatin remodeling complex that is related to the yeast SWI/SNF chromatin remodeling complex [49–51]. Overexpression of CREST inhibits neurite outgrowth in cultured neurons and causes retina degeneration in transgenic Drosophila [48,52]. Also, CREST co-immunoprecipitates with FUS in neuron lysates [48,52]. In cultured cells, transformed CREST forms predominantly nuclear dots with some cytoplasmic foci. Also, like other ALS associated proteins, CREST is recruited to stress-induced ribonucleoprotein particle (RNP) granules [52,53]. While many proteins must reversibly aggregate in physiological granules as part of their normal cellular function, this ability to aggregate could lead to the formation of pathological aggregates. Indeed, TDP-43 and FUS have prion-like domains and have been found in cytoplasmic aggregates in the neurons of patients with ALS. While no such aggregates have yet been reported for CREST, it has an unstructured prion-like domain predicted to form amyloid-like aggregates [48]. CREST in cell culture was slightly resistant to Triton-X100 but was solubilized by SDS; in addition it formed nuclear dots in a concentration-dependent manner [52]. Here we establish a new ALS model in yeast. We show that this human chromatin remodeling complex component and transcriptional activator is toxic and forms largely nuclear aggregates that stain with Thioflavin T when expressed in yeast. We also show that CREST toxicity is increased by the endogenous yeast [PIN+] prion and reduced by deletion of the HSP104 chaperone that is required for propagation of many yeast prions [4]. Deletion of the TDP-43 toxicity modifier, PBP1, likewise reduces CREST toxicity and this finding was also confirmed in a CREST transgenic fly model. Both hsp104Δ and pbp1Δ alter CREST aggregation. Finally, overexpression of PBP1 causes a large portion of CREST to leave the nucleus and co-localize with PBP1 containing cytoplasmic dots in yeast. Likewise, in mammalian cells, cytoplasmic aggregates of CREST sequester ATXN2. Overall, our results establish PBP1/ATXN2 as a potent modifier of CREST toxicity in vivo.
  Growth of yeast transformed with control GAL-GFP vectors was compared with yeast expressing fusions of human CREST cDNA and GFP from a GAL promoter on three types of vectors: a Yeast CEN plasmid (YCp) containing a centromere and expressed as one or a few copies in each cell (Fig 1A); a Yeast Episomal plasmid (YEp) containing elements of the endogenous yeast 2μ episome and expressed in high copy in some cells but absent in other cells (Fig 1B); or a Yeast Integrating plasmid (YIp) that was integrated into the genome (Fig 1C). Growth inhibition is evident but slight when CREST-GFP was expressed from a CEN vector (Fig 1A) and more inhibition is seen when episomal (Fig 1B) or integrating vectors (Fig 1C) were used. Expression of the GAL controlled genes was turned on with a constitutively expressed fusion of the human estrogen receptor hormone-binding domain, the yeast GAL4 DNA-binding domain and the VP16 viral transcriptional activator, which was activated by the addition of β-estradiol [54]. The high level of β-estradiol used here (2 μM) in glucose media results in more expression from the GAL promoter than that obtained by traditional induction on 2% galactose without β-estradiol [55]. Note the reduced induction of CREST caused by galactose relative to β-estradiol results in reduced toxicity (S1 Fig). Similar to mammalian cells [52], CREST-GFP expressed in yeast largely appeared as dots in nuclei, but smaller cytoplasmic foci were also visible and were more frequent after longer induction times (Fig 2A). Nuclear localization was confirmed by using DAPI stain or a constitutively expressed nuclear HTB1-mCh marker (Fig 2B and S2 Fig) which co-localized with CREST-GFP. CREST foci also stained with Thioflavin T that marks amyloid-like protein (Fig 2C). For controls we show TDP-43 and FUS aggregates that, respectively do not and do, stain with Thioflavin T [56]. In addition, the bulk of the CREST protein is found in the pellet of lysates while the non-aggregated proteins, GFP and PGK, remain in the supernatant (Fig 2D). When CREST was overexpressed in mammalian cells, it formed large dot-like nuclear structures and was also present in cytoplasmic aggregates [52]. When CREST is expressed in yeast for 5–6 h, most of the protein is found in nuclear dots with just occasional cytoplasmic foci (see Figs 2 and 3 left). However, when cells with nuclear CREST are stressed by incubation at high temperature there is a dramatic increase in the appearance of cytoplasmic foci (Fig 3 middle) that partially co-localize with Ded1-mCh stress granule and EDC3-mCh P-body markers. Following a return to 30°C, cytoplasmic CREST aggregates disappear along with stress granules and P-bodies (Fig 3 right). Since the presence of the [PIN+] prion causes enhanced toxicity of the human genes huntingtin/polyQ, TDP-43 and FUS [28,36,37,57], we asked if [PIN+] would likewise increase toxicity of CREST and found that it did (Fig 4A). However, CREST and [PIN+] aggregates did not co-localize (Fig 4B). Also, CREST aggregated in both [PIN+] and [pin-] cells (Figs 2A and 4D). Interestingly, CREST toxicity was reduced even further when the HSP104 chaperone required for the propagation of many yeast prions [4,58] was deleted in [pin-] cells (Fig 4C left). Deletion of HSP104 also caused the appearance of many nuclei with diffuse CREST-GFP with or without dots instead of nuclear CREST-GFP dots on a black background, and reduced the number of cells showing any cytoplasmic CREST aggregation (Fig 4D). CREST-GFP was found largely in the pellet in lysates of both hsp104Δ and HSP104 yeast (Fig 4E). The higher level of CREST-GFP in the hsp104Δ cultures (Fig 4C right) could reflect the reduced toxicity of CREST. While working with GAL-CREST-GFP and GAL-GFP transformants, we noticed that expression of CREST-GFP caused cells in liquid culture to settle very rapidly compared to the GFP controls. In a measured experiment, we found that overexpression of CREST caused flocculation (cell clumping), while overexpression of TDP-43 or FUS did not (Fig 5A). Since CREST increased yeast flocculence just as the non-prion form of the SWI1 chromatin remodeling protein does [59], we hypothesized that CREST, like SWI1, remodels yeast chromatin structure causing a release of silencing of telomerically located and epigenetically controlled [60] FLO genes, resulting in flocculation. To test this we examined the effect of CREST on the telomerically located FLO1 and the epigenetically controlled FLO11 promoters, using ura3Δ strains with either the FLO1 or FLO11 open reading frames (ORFs) replaced with the URA3 ORF reporter [59]. In the absence of CREST these strains were auxotrophic for uracil indicating that both the FLO1 and FLO11 promoters were off. However, upon CREST expression on galactose medium, both strains gained the ability to grow in the absence of uracil, indicating that the FLO1 and FLO11 promoters were indeed both activated by CREST (Fig 5B). Considerable evidence suggests that amyloid-like aggregates of one protein can facilitate the de novo aggregation of certain heterologous proteins. Such a phenomenon could be an important risk factor for disease. While there are no yeast proteins that are homologous to the human CREST, the yeast protein with the most similarity to CREST, using a BLAST search, is RNQ1, the [PIN+] protein. This is likely due to the extensive QN-rich regions in both proteins. Thus, we asked if aggregates of CREST, like [PIN+] and other QN-rich aggregates, could facilitate the de novo aggregation of SUP35 to form the [PSI+] prion. In [PIN+] but not [pin-] cells, transient overexpression of SUP35NM-GFP causes the appearance of large fluorescent SUP35NM-GFP rings and converts [psi-] cells into [PSI+] [8,17,55,61]. Here we detect conversion into [PSI+], which causes readthrough of nonsense codons, by the appearance of adenine prototrophy despite the presence of the nonsense mutation ade1-14. We found that unlike [PIN+], or overexpression of a variety of QN-rich yeast proteins [8], overexpression of CREST did not cause the appearance of fluorescent SUP35NM-GFP rings or adenine prototrophy. This was true even when PBP1 was also overexpressed (S3 Fig), which, as described below in the last research section, drives CREST-DsRED aggregates into the cytoplasm. Overexpression of yeast’s PBP1, a homolog of human ATXN2, enhances, while PBP1 deletion reduces, the toxicity of TDP-43 expressed in yeast [42]. This led to the discovery that ATXN2 intermediate-length polyglutamine expansions, which are associated with increased ATXN2 protein levels, are a risk factor for ALS [42]. Work in a variety of model organisms including mice, now suggests that ATXN2 is a modifier of some neurodegenerative diseases [43]. We thus tested the effect of PBP1 level on the toxicity of CREST in yeast. Overexpression of PBP1 itself was toxic in our system (Fig 6A) so the effect on CREST toxicity could not be determined. However, we clearly show that deletion of PBP1 reduces toxicity in either a [PIN+] or [pin-] background (Fig 6B) and this is associated with an increase in CREST-GFP solubility (Fig 2D). Indeed, lysates of pbp1Δ cells expressing CREST-GFP had more GFP reactive protein in the supernatant vs. pellet fractions while the reverse was true for PBP1 lysates. Furthermore the CREST-GFP in pbp1Δ supernatant showed a lot of degradation compared with CREST-GFP in the pellet or in either supernatant or pellet fractions of the isogenic PBP1 strain (Fig 2D). This is likely because soluble CREST-GFP is more susceptible to degradation than aggregated CREST-GFP found in the pellet. We also saw a significant reduction in the number of cells with cytoplasmic CREST aggregates in the presence of pbp1Δ (Fig 6C). Taken together the data imply that a smaller fraction of total CREST-GFP is aggregated in either nuclei or cytoplasm in the absence of PBP1. In order to validate the observed effect of the ATXN2 ortholog PBP1 in an independent in vivo model, we used transgenic Drosophila which possesses an ortholog of the human ATXN2 gene, dAtx2. Overexpression of untagged wild-type CREST using GAL4-UAS system in transgenic lines results in severe retinal degeneration [52]. For our studies we used dAtx2 deletion fly line (dAtx2 06490) [62]. Homozygous deletion of dAtx2 was reported to cause disorganized and decreased photoreceptor neurons, but there was no effect in the heterozygous state [62]. We crossed CREST overexpressing (transgenic, tg) flies with heterozygous dAtx2 flies (dAtx206490-/+) and analyzed the double tg progeny. Histological analysis confirmed normal retinal morphology in dAtx206490-/+ flies, whereas complete disruption of the regularly ordered arrays of photoreceptor neurons was observed in the retina in CRESTwt tg flies, consistent with the previous report [52]. Strikingly, this severe retinal degenerative phenotype in CRESTwt tg flies was largely rescued by downregulation of dAtx2 (CRESTwt tg; dAtx206490- /+ line) (Fig 7A and 7B). This rescue was not caused by reducing the expression of CREST (Fig 7C). Thus, human CREST toxicity in flies is largely mediated by endogenous dAtx2. Finally, we asked whether potentiated toxicity of CREST in the presence of PBP1 might be linked to the aggregation-prone nature of the two proteins. We found a dramatic increase in cytoplasmic CREST dots when PBP1 was overexpressed in yeast (Fig 8A). This was not due to an increase in CREST protein level (Fig 8B). We also found the CREST-DsRed dots co-localized with PBP1-GFP in cytoplasmic foci (Fig 8C). However these foci do not appear to be RNP granules because they generally lack DED1 and EDC3 (S4 Fig). We next examined the localization of human ATXN2 in CREST-overexpressing human cells. CREST-GFP forms nuclear aggregates in cells expressing high levels of the protein, and in the ~10% of cells with the highest levels of CREST-GFP, cytoplasmic aggregates also develop [52]. We found that cytoplasmic CREST-GFP aggregates sequester endogenous ATXN2 in human neuroblastoma cells under these conditions (Fig 8D middle), whereas in non-transfected (NT) cells and in cells lacking these aggregates, ATXN2 remains diffusely distributed (Fig 8D, top and bottom). Thus, CREST and PBP1/ATXN2 co-localize in the cytoplasm, which may serve as one of the mechanisms of PBP1/ATXN2-mediated toxicity.
  Our data show that human CREST expressed in yeast shares properties with other ALS-associated proteins and yeast prions: CREST, FUS and yeast prions form amyloid-like foci; CREST, TDP-43 and FUS toxicity and yeast prion appearance are enhanced by the yeast prion [PIN+]; TDP-43 and CREST toxicity is reduced by deletion of PBP1; CREST, TDP-43 and FUS are associated with cytoplasmic RNP granules. Like maintenance of yeast prions, but unlike effects on FUS or TDP-43 [31,32], CREST toxicity and aggregation is dependent on the presence of HSP104 chaperone. In mammals, endogenous CREST is a member of a chromatin remodeling complex and thereby activates transcription. When expressed in yeast, we found that CREST releases telomeric silencing and causes flocculation. Thus we propose that even in yeast the heterologous CREST protein remodels chromatin causing transcriptional activation of telomerically located genes including those that cause flocculation (e. g. FLO1, FLO5, FLO9 and FLO10) as well as the epigenetically controlled gene FLO11. Likewise, a yeast chromatin remodeling complex protein, SWI1, is required for the expression of telomerically located and epigenetically controlled FLO genes [63]. Interestingly, SWI1 can form infectious prion aggregates [12] reminiscent of the amyloid CREST aggregates seen here. We first described [PIN+], the prion form of the RNQ1 protein, because its presence allowed the efficient conversion of the SUP35 protein to its prion form, [PSI+] [8,17,64]. It was later shown that [PIN+] is also required for the efficient aggregation and toxicity of polyQ in yeast [28]. The mechanisms causing these effects are still unknown. Some data support the hypothesis that the [PIN+] aggregates of RNQ1 cross-seed aggregation of heterologous proteins such as SUP35 and polyQ. Likewise some, but not all, evidence suggests that the [PIN+] aggregates bind proteins such as chaperones that would otherwise inhibit aggregation of the heterologous SUP35 or polyQ protein, thereby enhancing their aggregation [18–25,27] We recently showed that toxicity of TDP-43 and FUS is slightly enhanced by the presence of [PIN+] [37,57]. Both of these proteins contain Q/N-rich regions and co-aggregate with polyQ disease protein alleles of huntingtin [65,66]. Surprisingly, we did not detect a clear effect of [PIN+] on TDP-43 or FUS aggregation. Likewise, CREST is a Q-rich protein. We show here that the toxicity of CREST is also slightly enhanced by [PIN+], again with no clear effect on aggregation. Apparently the toxicity of the aggregates can be altered without a noticeable change in their appearance. On the other hand, deletion of either HSP104 or PBP1, which both cause dramatic rescues of CREST toxicity, were each accompanied by reduced frequency of CREST cytoplasmic foci, more diffuse CREST in nuclei and, for pbp1Δ, reduced CREST sedimentation. Surprisingly, despite the fact that we clearly detect more diffuse CREST in the presence of hsp104Δ, there was no increase in CREST degradation or in the level of CREST in supernatant fractions as was seen for pbp1Δ. Also, immunoblots of pbp1Δ lysates reproducibly show that CREST-GFP in the supernatant is degraded much more in the pbp1Δ vs. WT PBP1 strain. CREST-GFP in the pellet fractions was stable in either PBP1 or pbp1Δ strains. This suggests that pbp1Δ causes more CREST to remain unaggregated and subject to degradation. Indeed there was more CREST-GFP plus GFP breakdown product in the supernatant vs. pellet in the pbp1Δ while the reverse was true for the PBP1 strain. While pbp1Δ also rescues toxicity of TDP-43, effects on TDP-43 aggregation have not been reported [42]. Since the HSP104 chaperone is required to shear the [PSI+] prion aggregates thereby creating ends where growth occurs, inhibition of HSP104 initially causes [PSI+] prion polymers to enlarge and, for newly made SUP35-GFP, to not efficiently join the aggregates remaining diffuse instead [67]. Possibly something similar is happening to CREST-GFP in the absence of HSP104. It is also possible that HSP104 is directly involved in CREST aggregate formation. A recent finding that deletion of PBP1 causes a reduction in the level of RNQ1 protein [68] could have explained the reduced CREST, TDP-43 and FUS toxicity seen in pbp1Δ cells if the lowered RNQ1 level reduced the potency of [PIN+]. However, we show here that this is not the case because pbp1Δ reduces CREST toxicity even in the absence of [PIN+] (Fig 6E). It remains possible that toxicity is reduced by pbp1Δ by reducing RNQ1 levels regardless of [PIN+] status. We have shown that the presence of certain QN-rich aggregates can substitute for [PIN+] and allow the efficient induction of [PSI+] by overexpression of SUP35 [8]. This fact, plus the observation that RNQ1, the [PIN+] prion protein, although not a homolog, is the yeast protein most similar to CREST, prompted us to ask if overexpression of CREST could substitute for [PIN+]. However, CREST did not provide any [PIN+] activity even when PBP1 was overexpressed driving CREST into the cytoplasm. Prion aggregates enhance de novo formation of heterologous prion, presumably by a cross-seeding mechanism. Curiously, however, if heterologous prion is already present, prion aggregates instead promote loss of the heterologous prion [20]. One mechanism for this has been shown to be prion aggregate binding to, and therefore titrating, chaperones needed to propagate the heterologous prion [24]. Likewise, CREST has been shown to suppress polyQ-mediated huntingtin aggregation and toxicity [69]. Many of the ALS associated proteins, e. g. TDP-43, FUS and TAF15 are soluble nuclear RNA/DNA binding proteins that regulate mRNA splicing and/or stability and contain prion-like unstructured domains and nuclear/cytoplasmic trafficking signals. In neurons of patients with ALS/FTD these proteins have been found in cytoplasmic aggregates instead of their normal nuclear location. This suggests that impaired nucleocytoplasmic transport may be a general mechanism for neurotoxicity [3,70–75]. Indeed, impairing nuclear import of TDP-43 enhances neurotoxicity [76]. While TDP-43 and FUS quickly form cytoplasmic aggregates in yeast with little or no nuclear localization [31,32,37,57,77–79], we found here that CREST appeared in nuclear aggregates with only occasional cytoplasmic foci. While the ALS-associated proteins TDP-43, FUS and SOD1 form aggregates in patients, model systems and in vitro, these aggregates are sometimes but not always classical amyloids. In yeast, aggregates of FUS, but not TDP-43, stain with Thioflavin T that is indicative of amyloid-like protein [56]. Although both nuclear and cytoplasmic CREST foci appear to stain with Thioflavin T, it is nonetheless tempting to speculate, by analogy with FUS and TDP-43, that the CREST cytoplasmic foci are more toxic than nuclear CREST. Indeed reduction of CREST-GFP toxicity caused by deletion of either PBP1 or HSP104 also reduced the frequency of cells with cytoplasmic CREST-GFP aggregates. We found that heat stress induced CREST to leave the nucleus and partially co-localize with RNP granules. This is consistent with previous findings in mammalian cells that CREST becomes mislocalized to the cytoplasm under stressful conditions (oxidative stress) and becomes recruited to cytoplasmic stress granules [52]. Furthermore, as seen for classic stress granule proteins, CREST returned to the nucleus in yeast following the heat stress. Many proteins associated with neurodegenerative disease contain intrinsically disordered regions that are required for their appearance in RNP granules. Evidence has been accumulating that RNP granules are incubators for the formation of pathogenic protein aggregates associated with neurodegenerative disease. It has been proposed that localization of proteins such as TDP-43, FUS and C9ORF72-encoded dipeptide repeat GR50 in high concentration in stress granules likely in a gel form, promotes their conversion into pathogenic amyloid-like aggregates [80–84]. In support of this model, RNP-granule formation has been shown to be strongly associated with neurodegeneration. This was accomplished with the aid of ATXN2, a nucleic-acid-binding protein that is required for RNP-granule formation. Three ALS associated proteins, TDP-43, FUS and C9ORF72-encoded GR dipeptide, have been shown to co-localize with ATXN2 in RNP granules [42,80] in yeast, human or Drosophila S2 cells. Also, either reducing the level of ATXN2 or deleting one of its intrinsically disordered regions both reduced RNP-granule formation and reduced neurodegeneration caused by TDP-43, C9ORF72 dipeptides or FUS [80,85–87]. These results help explain why expansions of the polyQ regions in ATXN2 that increase the stability, and thus likely the level of ATXN2, are associated with neurodegenerative disease (susceptibility to ALS and type 2 spinocerebellar ataxia). Here, we report similar effects of PBP1/ATXN2 on CREST: deletion of PBP1 reduces toxicity of CREST in yeast and fly models of CREST proteinopathy; ATXN2 is recruited into cytoplasmic CREST aggregates in mammalian cells and overexpression of PBP1 causes CREST to co-localize with PBP1 in cytoplasmic foci in yeast. Overexpression of PBP1 has previously been shown to induce the formation of cytoplasmic PBP1 foci [88] that recruit TORC1 thereby down regulating TORC1 function [89]. PBP1 foci may similarly attract CREST which may promote CREST’s conversion to a more toxic form. In sum, we showed that CREST is yet another protein, in addition to TDP-43, that is highly sensitive to ATXN2 dosage indicating that ATXN2 likely has a more general role as a modifier of neurotoxicity. Our results extend the spectrum of ALS-associated proteins affected by ATXN2 to include CREST. This supports the hypothesis that therapies that target ATXN2 may be effective for a wide range of neurodegenerative diseases.
  Yeast strains and plasmids used are listed in Tables 1 and 2. Unless otherwise stated, yeast strain BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) was used. Yeast transformation was by the lithium acetate method [90]. L3491 bearing an integrated copy of ADH1-HTB1-mCh was used to check CREST nuclear localization [37]. FLO1 (L3580) and FLO11 (L3582) promoter reporter strains were respectively made from DY755 and DY758. DY755 and DY758 are Ura+ because FLO8, which is disrupted in these strains, is required for regulation that inhibits the FLO1 and FLO11 promoters [59,91]. We obtained pop-outs of the HIS3 disruptions of FLO8 by selecting for Ura- revertants on FOA plates [92] and checking these for those that had become His-. Several such pop-outs were obtained and tested and they all behaved identically. Unless otherwise stated, all overexpression plasmids were driven by the GAL1 promoter. Gateway technology [93] was used to make CREST entry clone pDONR-CREST with BP reactions between pDONR221 (Invitrogen, Cat# 12536017) and a CREST fragment amplified from pC1-CREST with PCR. The CREST fragment in pDONR-CREST was further transferred to YEpGAL-GFP, YCp-GAL-GFP, YEpGAL-DsRED and YIpGAL-GFP to respectively build YEpGAL-CREST-GFP, YCpGAL-CREST-GFP, YEpGAL-CREST-DsRED and YIpGAL-CREST-GFP with LR reactions. To increase expression of GAL1 controlled CREST, pβ-est, containing human estrogen receptor hormone-binding domain fused to the GAL4 DNA binding domain and the VP16 viral transcriptional activator (hER) was used. Standard media and growth conditions were used [94,95]. All liquid-culture experiments were carried out with mid-log-phase cells. Complex (YPD) yeast media contained 2% dextrose. Synthetic medium lacked a specific component, e. g. leucine, and contained 2% dextrose, e. g. , SD-Leu (-Leu medium) or 2% galactose without (SGal-Leu) or with 2% raffinose (SRGal-Leu). Cells co-transformed with pβ-est, containing hER, were grown in β-estradiol (2 μM) in glucose media to turn on expression of GAL1 controlled CREST [54], [55]. FOA medium containing 12 mg/l 5-fluoro-orotic acid and 12 mg/l uracil was used to select for Ura- cells [92]. To prevent the accumulation of suppressor mutants that reverse CREST toxicity, pGAL1-CREST transformants were maintained on plasmid selective SD medium where CREST was not expressed. Patches were then replica-plated onto synthetic glycerol (2%) to identify petites that were dropped from further study. To analyze growth, non-petite transformants from SD plates were normalized in water to an OD600 of 2 and serially diluted 10X. Finally about 5 μl of diluted cell suspensions were spotted on plasmid selective SD, SGal or SD + β-estradiol using an MC48 (Dan-kar Corp, MA) spotter. CREST was detected in pre-cleared lysates separated by SDS-PAGE and blotted as described previously [96]. For analysis of proteins in total lysate vs. supernatant and pellet, the pellet from 90 μg of total lysate centrifuged at 50K rpm for 6 min was resuspended in water to the initial volume of total lysate centrifuged. The same sample volumes of total lysate, supernatant and pellet were then separated by SDS-PAGE. Blots were developed with GFP mouse antibody from Roche Applied Science (Indianapolis, IN) and PGK antibody from life technologies (Frederick, MD). Fluorescently labeled protein aggregates, were visualized in cells with a Nikon Eclipse E600 fluorescent microscope (100X oil immersion) equipped with FITC, YFP and mCh (respectively, chroma 49011,49003 and 49008) filter cubes. To visualize nuclei, cells with the integrated HTB1-mCh nuclear marker were used [37] or nuclei were stained with 1 μg/ml 4′, 6′‐diamidino‐2‐ phenylindole (DAPI) in 1XPBS for 10 min following fixing and permeabilizing cells for 1 h in 60% ethanol in PBS. Flocculation was assayed by resuspending a sample of each 2 d culture grown in plasmid selective SRGal in 50 mM EDTA in water. Since flocculation requires the presence of Ca2+ ions [97], EDTA removed clumping allowing cells’ OD600 to be accurately measured. Then another sample of cells that was not treated with EDTA was appropriately normalized to OD600 = 10. Cells were photographed after they were allowed to settle for 15 min. The level of precipitation of cells and the reduction in the OD of the supernatants (diluted 10X) is a measure of the flocculence. Yeast cells were stained with Thioflavin T according to a protocol adapted from [31] with the addition of two extra washes in PMST [0. 1 M KPO4 (pH 7. 5), 1 mM MgCl2,1 M Sorbitol, 0. 1% Tween 20]. Generation of the human CREST line used in this study has been described earlier [52]. gmr-GAL4, UAS-lacZ and dAtx2 06490 lines were obtained from the Bloomington Drosophila stock center. Fly stocks were raised on standard Drosophila medium at 20°C. Crosses between the Drosophila strains were carried out at 25°C using standard procedures. For histochemical analyses, heads of 5-day-old adult transgenic flies were dissected, collected, briefly washed in phosphate buffered saline (PBS), and fixed with 4% paraformaldehyde containing 0. 1% Triton X-100 at room temperature for 2 h. After a brief wash in PBS, tissues were dehydrated by graded ethanol, cleared in butanol and embedded in paraffin. Four-micrometer thick coronal sections were stained with hematoxylin and eosin (H&E). Between 8 and 10 flies were analyzed per genotype, and 3 sections were analyzed for each fly. For Western blot analysis, heads of 5-day-old flies were lysed in Laemmli sample buffer containing 2% SDS. Anti-CREST (Proteintech, 12439-1-AP) and anti-α-tubulin (Sigma, DM1A) antibodies were used. WT CREST in pEGFP-C1 vector (Clontech) was used for CREST-GFP overexpression [52]. SH-SY5Y neuroblastoma cells were cultured in 1: 1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 medium supplemented with 10% FBS, 50 U/ml penicillin-streptomycin, and 500 μM L-glutamine (all Invitrogen). Cells were fixed with 4% cold paraformaldehyde for 15 min at RT and permeabilized with methanol. Cells were incubated with a primary anti-ATXN2 antibody (rabbit polyclonal, 21776-1-AP, Proteintech) diluted in PBS-T containing 5% goat serum at room temperature for 2 h. Fluorochrome-conjugated secondary antibody (AlexaFluor, Life Technologies) diluted 1: 1,000 in PBS-T were applied at RT for 1. 5 h. Nuclei were stained with DAPI and mounted on glass slides using Immu-Mount (Thermo Scientific). Fluorescent images were taken using a BX57 fluorescent microscope equipped with ORCA-Flash 4. 0 camera (Hamamatsu) and cellSens Dimension software (Olympus). Figures were prepared using Photoshop CS3 software.
summary: Mutations in the calcium-responsive transactivator (CREST) protein have been shown to cause amyotrophic lateral sclerosis (ALS). Here we show that the human CREST protein expressed in yeast forms largely nuclear aggregates and is toxic. We also show that the HSP104 chaperone required for propagation of yeast prions is likewise required for CREST toxicity. Furthermore deletion of HSP104 affects CREST aggregation. ATXN2, previously shown to modify ALS toxicity caused by mutations in the TDP-43 encoding gene, also modifies toxicity of CREST expressed in either yeast or flies. In addition, deletion of the yeast ATXN2 ortholog reduces CREST aggregation. These results extend the spectrum of ALS associated proteins whose toxicity is regulated by ATXN2, suggesting that therapies targeting ATXN2 may be effective for a wide range of neurodegenerative diseases.
keywords: pharmacologic analysis
  medicine and health sciences
  pathology and laboratory medicine
  chemical compounds
  neurodegenerative diseases
  amyotrophic lateral sclerosis
  light microscopy
  carbohydrates
  organic compounds
  glucose
  toxicology
  toxicity
  fungi
  yeast estrogen screen
  microscopy
  molecular biology techniques
  pharmacology
  motor neuron diseases
  research and analysis methods
  fluorescence microscopy
  hyperexpression techniques
  chemistry
  molecular biology
  molecular biology assays and analysis techniques
  yeast
  gene expression and vector techniques
  eukaryota
  organic chemistry
  neurology
  monosaccharides
  biology and life sciences
  physical sciences
  organisms
title: Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies