article: Despite the known burden of neglected tropical diseases (NTDs) on child health, there is limited information on current efforts to increase pediatric therapeutic options. Our objective was to quantify and characterize research activity and treatment availability for NTDs in children in order to inform the prioritization of future research efforts. We conducted a review of the World Health Organization’s (WHO) International Clinical Trials Registry Platform to assess research activity for NTDs. The burden of disease of each NTD was measured in terms of disability adjusted life years (DALYs), which was extracted from the Global Health Data Exchange. First- and second-line medications for each NTD were identified from WHO guidelines. We reviewed FDA drug labels for each medication to determine whether they were adequately labeled for use in children. Descriptive statistics, binomial tests, and Spearman’s rank order correlations were calculated to assess research activity compared to burden of disease. Children comprised 34% of the 20 million DALYs resulting from NTDs, but pediatric trials contributed just 17% (63/369) of trials studying these conditions (p<0. 001 for binomial test). Conditions that were particularly under-represented in pediatric populations compared to adults included rabies, leishmaniasis, scabies, and dengue. Pediatric drug trial activity was poorly correlated with pediatric burden of disease across NTDs (Spearman’s rho = 0. 41, p = 0. 12). There were 47 medications recommended by the WHO for the treatment of NTDs, of which only 47% (n = 22) were adequately labeled for use in children. Of the 25 medications lacking adequate pediatric labeling, three were under study in pediatric trials. There is a substantial gap between the burden of disease for NTDs in children and research devoted to this population. Most medications lack adequate pediatric prescribing information, highlighting the urgency to increase pediatric research activity for NTDs with high burden of disease and limited treatment options.
  Neglected tropical diseases (NTDs) are a group of poverty-associated parasitic, bacterial, and viral conditions that affect more than 1. 4 billion people worldwide [1]. Collectively, NTDs lead to over 500,000 deaths annually and also cause substantial morbidity, particularly among impoverished populations in low- and middle-income countries [2–4]. Several conditions disproportionately affect children compared to adults [5], and children are often simultaneously infected with multiple parasitic NTDs [6]. In sub-Saharan Africa, it is estimated that as many as 50 million children are infected with hookworm alone and in Southeast Asia, nearly 120 million pre-school and school age children require periodic treatment for soil-transmitted helminth infections [7,8]. The burden of NTDs in children is compounded by the general underrepresentation of pediatric populations in clinical trials and drug development [9]. This is principally a function of the oft limited financial incentives for drug development in children, as well as the greater scientific, ethical, and regulatory challenges in conducting trials in this population [9]. Despite the substantial and ongoing burden of NTDs on child health, there is limited information on current efforts to increase pediatric therapeutic options [5]. The objective of this study was to quantify and characterize research activity for the prevention and treatment of NTDs in children in order to inform strategic initiatives and guide the prioritization of future research efforts. We examined the alignment of research activity with burden of disease in children across NTDs and also determined the availability of pediatric prescribing information to support the use of first- and second-line therapies for NTDs in children.
  We conducted a review of trials registered in the World Health Organization’s (WHO) International Clinical Trials Registry Platform (ICTRP) to assess research activity for NTDs. NTDs were defined based on the WHO’s 2017 list of NTDs, which includes the recent additions of chromoblastomycosis and other deep mycoses, scabies and other ectoparasites, and snakebite envenoming [10]. The Institutional Review Board at Boston Children’s Hospital determined that the study was exempt from review. Global disease burden for each NTD was determined based on reports of the Global Health Data Exchange [11,12]. These data are collected from 195 countries to create a comprehensive catalog of health-related data, quantifying the prevalence and impact of diseases and conditions on global public health. One of the measures provided for each disease is disability-adjusted life years (DALYs), which represent years of life lost due to disability or death. DALYs are a commonly-used metric in assessing the impact of diseases, particularly those with a chronic component [13], and have been previously used to evaluate the allocation of research and other resources across diseases [14–16]. We extracted DALYs from all regions for 2006 to 2016 and determined average yearly DALYs for each NTD. To obtain DALYs for children 0 to17 years of age, we relied on the age groups provided in the dataset and summed DALYs for children <14 years of age and three-fifths of DALYs for the age group 15 to 19 years [17]. Clinical trials for NTDs were identified in the WHO ICTRP. This publicly-accessible trial registry was created following the Ministerial Summit on Health Research in 2004 to create a single point of access for information on clinical trials globally [18]. The WHO ICTRP includes data from 17 registries from both high-income and low- and middle-income countries and provides data on roughly 430,000 trials (S1 Appendix). We also extracted data from ClinicalTrials. gov, a public trial registry administered by the U. S. National Institutes of Health [19]. Since trial records in ClinicalTrials. gov contain additional details not available in the WHO ICTRP, all trials identified in the WHO ICTRP were cross-referenced to ClinicalTrials. gov, and when available, data extracted from this registry as well. We searched the WHO’s ICTRP Search Portal [20] on a single day (February 9,2018) to identify all trials involving NTDs conducted between January 1,2006 and December 31,2016. For conditions with more than one commonly used name (e. g. Chagas and American Trypanosomiasis), all names were searched in the WHO ICTRP (S2 Appendix). Trials were included in the analysis if they studied a drug or vaccine for an NTD, were registered during the study period, were interventional, and were phase 2 to 4 trials. We excluded trials that did not primarily focus on the prevention or treatment of NTDs and those that were withdrawn or terminated. Pediatric trials were defined as those that exclusively studied children 0–17 years of age or those that also included adults but in which the mid-point of the age eligibility range was <18 years [21]. For trials selected in our final cohort, we extracted trial name, registration number, age eligibility of participants, sponsor, enrollment number, duration, randomization status, and geographic location. Sponsors were categorized as industry, government, hospital or university, or other non-profit organization. For randomization status, trials were categorized as randomized or not randomized based on the listed study design or trial description. Geographic location was determined from the listed study country or countries, and categorized based on the World Bank’s Country and Lending Group classification [22]. Recommended treatments for NTDs were identified from WHO fact sheets published in 2017 (S3 Appendix) [23]. As there were no 2017 fact sheets for buruli ulcer, dengue, and leishmaniasis, the most recently published WHO guidelines for these conditions were used [24–26]. For chromoblastomycoses, there was no WHO-recommended first- or second-line treatments, and a widely-cited review paper was used to determine treatment options [27]. We reviewed the US Food and Drug Administration (FDA) drug labels for each WHO-recommended medication to determine status of pediatric labeling [28]. Medications were classified as adequately labeled for use in pediatric populations if labels stated that the medication was approved for pediatric use, provided dosing information for use in children, or included safety and/or efficacy information for pediatric populations [29]. If none of those were available, the medication was not considered adequately labeled for use in children. To quantify the dissemination of results from pediatric NTD trials, we searched for published articles for each of the trials in our cohort. This search was limited to trials completed by December 31,2015 in order to allow for a minimum period of 26 months from trial completion to publication. A publication was defined as a report published in a peer-reviewed journal presenting results on at least one of the trial’s primary endpoints. We first searched for publications automatically indexed to the trial record in ClinicalTrials. gov. If none were available, we performed a systematic search in PubMed, using trial registration number, trial title, author names, institutions, and study keywords. We reviewed the abstract and, if necessary, the full text to link corresponding trials to publications based on data presented. If no publication was identified in PubMed, additional searches were performed in Embase and GoogleScholar. For industry-sponsored trials, we reviewed the company website for information on publications. For trials with more than one resulting publication, we selected the earliest publication. Descriptive statistics were used to characterize pediatric trials for NTDs. We used the binomial test to compare the proportion of global burden of disease that was pediatric to the proportion of clinical trials that was pediatric, collectively and for each NTD. For example, for an individual NTD, we divided the number of DALYs representing pediatric disease burden by the total number of DALYs for the condition, and compared this proportion to the proportion obtained when dividing the number of pediatric trials by the total number of trials for the condition. Conditions without burden of disease data were excluded from this analysis. As a measure of pediatric research activity for each NTD, we calculated the ratio of pediatric DALYs per pediatric trial. We used Spearman’s rho test to assess the correlation between pediatric global burden of disease in DALYs for NTDs and number of trials as well as total number of enrolled participants in all trials for each condition. To allow for representation of conditions that did not have any clinical trials, we added one trial to each condition. All analyses were conducted in Stata/SE version 13. 1 (Stata Corp, College Station, TX).
  Among the 63 pediatric trials, two-thirds (n = 42) were drug trials and a third (n = 21) were vaccine trials, of which the majority (n = 16) were dengue vaccine trials. 85% (n = 52) of the trials were randomized and 40% (n = 25) were funded by industry sponsors (Table 1). The median enrollment was 315 participants and the mean trial duration 2. 3 years. Of the 60 trials that provided information on study location, 65% (n = 36) were conducted in a single country, 25% (n = 15) in two countries, and 15% (n = 9) in three or more countries. Among these trials, 32% (n = 19) included sites in sub-Saharan Africa, 30% (n = 18) in Latin America and the Caribbean, 22% (n = 13) in East Asia and the Pacific, 7% (n = 4) in South Asia, 5% (n = 3) in Europe and Central Asia, 3% (n = 2) in the Middle East and North Africa, and 2% (n = 1) in North America. There were 45 trials that were completed as of December 31,2015, allowing for a minimum follow up period of 26 months and mean follow up of 44 months. Published articles reporting on the trial’s primary outcome were available for 80% (n = 36) of these trials, with an average of 1. 4 publications per trial (range 1–9). Nearly two-thirds (65%, n = 33) of the published articles were published in open access format. There were 15 NTDs with available DALYs. These NTDs resulted in approximately 20. 3 million DALYs averaged over the study period, of which 6. 8 million (34%) were attributable to disease in children. However, pediatric trials for NTDs comprised just 17% (63/369) of phase 2–4 trials for NTDs (p<0. 001 for binomial test comparing proportions of pediatric DALYs and trials) (Fig 2). Several conditions were particularly under-represented when considering burden of disease and research allocation between children and adults, including rabies, leishmanisias, scabies, and dengue. Others appeared to have higher proportions of research activity in children, including schistosomiasis, taeniasis, trachoma, and Chagas disease. In the case of schistosomiasis, which had the highest proportion of pediatric trials, the large number of pediatric trials represented primarily testing of a single product, praziquantel (10 of the 14 pediatric trials). Table 2 presents the ratios of pediatric DALYs per pediatric trial for each of the NTDs as a measure of research activity committed to each of the conditions. The ratios of DALYs per trial were least favorable for scabies, onchocerciasis, and lymphatic filariasis. When evaluating ratios of DALYs per participant, leishmaniasis, rabies, and scabies were comparatively neglected. The overall ratio of DALYs per trial across all NTDs was worst for children compared to adults (89 DALYs per pediatric trial vs. 41 DALYs per adult trial). Across NTDs, pediatric trial activity was moderately correlated with pediatric global burden of disease (Spearman’s rho = 0. 60, p = 0. 013). When assessing correlation based on trial enrollment, the correlation was slightly less (Spearman’s rho = 0. 49, p = 0. 15). We also examined the correlation limited to drug trials, which revealed a poor correlation between trial activity and disease burden (Spearman’s rho = 0. 41, p = 0. 12). However, pediatric vaccine trial activity was moderately correlated with pediatric global burden of disease (Spearman’s rho = 0. 53, p = 0. 03). There were 47 medications recommended by the WHO for first- and second-line treatment of NTDs, of which 22 (47%), representing 15 conditions, were adequately labeled for use in children (S2 Table). These 22 drugs included 8 for use in infants and children less than 2 years of age, 8 for children 2 to 10 years of age, and 6 for children age 10 and older. Only 7 of the 47 (15%) medications were available in pediatric formulations. Of the 25 recommended medications for which there was not adequate pediatric labeling, only three had pediatric trials in our cohort (nifurtimox, meglumine, and pentavalent antimonials). In addition, two of the NTDs—fascioliasis (one of the foodborne trematodiases) and mycetoma, chromoblastomycosis, and other deep mycoses—had no medications that were adequately labeled for use in children. There were no pediatric trials in our cohort studying these conditions. Table 3 presents the drugs and vaccines studied in pediatric NTD trials. Of the 11 NTDs for which there were interventional trials, 7 (64%) included trials for drugs or vaccines not currently labeled for use in children.
  There is a substantial gap between the burden of disease for NTDs in children and research devoted to this population, with certain conditions particularly under-represented compared to adult research investments. Most first- and second-line medications recommended for the treatment of these conditions lack adequate pediatric prescribing information, highlighting the urgency to increase pediatric research activity for NTDs with high disease burden and limited treatment options.
summary: Neglected tropical diseases are a group of poverty-associated parasitic, bacterial, and viral conditions. Collectively, they pose a substantial burden on child health, but there is limited information on current efforts to increase pediatric therapeutic options for these conditions. Understanding gaps in research activity and treatment options to reduce the global impact of neglected tropical diseases in children presents the opportunity to inform strategic initiatives and prioritize future research efforts. We analyzed trials in the World Health Organization’s International Clinical Trials Registry Platform, and found that pediatric trials comprised a disproportionately small number of drug and vaccine trials for neglected tropical diseases. Certain neglected tropical diseases, including rabies, leishmaniasis, scabies, and dengue, were particularly under-represented relative to their disease burden in children. We also determined that most medications recommended for the treatment of neglected tropical diseases lack critical data to support use in children, though few of these are currently being studied in pediatric trials. This study points to the urgent need to increase pediatric research activity for certain neglected tropical diseases that result in high disease burden and for which there are limited pediatric treatment options.
keywords: medicine and health sciences
  tropical diseases
  parasitic diseases
  pediatrics
  vaccines
  rabies
  ectoparasitic infections
  sexually transmitted diseases
  global health
  neglected tropical diseases
  infectious disease control
  public and occupational health
  infectious diseases
  zoonoses
  scabies
  protozoan infections
  leishmaniasis
  viral diseases
title: Neglected tropical diseases in children: An assessment of gaps in research prioritization