article: Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a devastating foliar disease. The Ug99 race group has combined virulence to most stem rust (Sr) resistance genes deployed in wheat and is a threat to global wheat production. Here we identified a coiled-coil, nucleotide-binding leucine-rich repeat protein (NLR) completely linked to the Ug99 resistance gene Sr21 from Triticum monococcum. Loss-of-function mutations and transgenic complementation confirmed that this gene is Sr21. Sr21 transcripts were significantly higher at high temperatures, and this was associated with significant upregulation of pathogenesis related (PR) genes and increased levels of resistance at those temperatures. Introgression of Sr21 into hexaploid wheat resulted in lower levels of resistance than in diploid wheat, but transgenic hexaploid wheat lines with high levels of Sr21 expression showed high levels of resistance. Sr21 can be a valuable component of transgenic cassettes or gene pyramids combining multiple resistance genes against Ug99.
  More than 700 million tons of wheat are produced every year, but further increases are needed to feed a rapidly growing human population. Reducing yield losses caused by wheat pathogens can contribute to this goal. Wheat stem rust, caused by the fungal pathogen Puccinia graminis f. sp. tritici (henceforth Pgt), poses an urgent threat to global wheat production since new virulent races have overcome widely deployed resistance genes. In 1998, a new Pgt race was detected in Uganda that was virulent to the widely deployed stem rust resistance genes Sr31 and Sr38 [1]. This race, also known as TTKSK using the North American system of nomenclature for Pgt races [2,3], was virulent to roughly 90% of the global wheat cultivars [4]. Since then, the Ug99 race group has spread to more than 13 countries in Africa and the Middle East [4–8] and acquired virulence to additional resistance genes (Sr24, Sr36, Sr9h and SrTmp [3,9–11]), prompting efforts to identify and clone effective resistance genes. The large and complex nature of the wheat genome has limited the number of cloned Ug99 resistance genes to six, including Sr35 [12], Sr33 [13], Sr50 [14], Sr22 [15], Sr45 [15], and Sr13 [16]. More resistance genes are necessary to diversify the combinations of Pgt resistance genes deployed as gene pyramids or in transgenic cassettes to provide durable resistance [15,17,18]. The stem rust resistance gene Sr21 was discovered in diploid wheat Triticum monococcum (genome Am), which is closely related to T. urartu, the progenitor of the A genome in polyploid wheat [19]. Sr21, which confers a more effective resistance to the Ug99 race group at high temperatures (20–24°C) than at low temperature (16°C) [20], was transferred to hexaploid wheat (T. aestivum) [21]. We previously mapped Sr21 to a 0. 19 cM interval on the central region of chromosome arm 2AmL and showed that this region includes a cluster of NBS-LRR resistance genes in the colinear region in the A genome of T. aestivum [20]. Here, we report the identification, validation and characterization of the wheat stem rust resistant gene Sr21, which encodes a coiled-coil nucleotide-binding leucine-rich repeat protein (NLR). Six pathogenesis-related (PR) genes showed increased transcript levels in Sr21-resistant genotypes inoculated with Pgt, only when plants were grown at high temperatures. This result provides a tentative explanation for the more effective Pgt resistance conferred by Sr21 at high temperatures. Finally, we identified five different resistance haplotypes of Sr21 and developed a diagnostic molecular marker to accelerate its deployment in breeding programs.
  The 0. 19 cM region on T. monococcum chromosome arm 2AmL including Sr21 is colinear with an 88-kb region in Brachypodium distachyon chromosome 5 flanked by genes Bradi5g22090 and Bradi5g22200 [20]. In the present study, we identified the wheat orthologs of six B. distachyon genes present in this colinear region (Bradi5g22100, Bradi5g22117, Bradi5g22146, Bradi5g22162, Bradi5g22179 and Bradi5g22187, Fig 1A, S1 Table) and mapped them in ten wheat lines with recombination events between Sr21 flanking markers FD527726 and EX594406 (Fig 1B). Using these new markers, Sr21 was mapped 0. 02 cM distal to CJ961291 and 0. 04 cM proximal to a group of four linked NLR pseudogenes (Cscnl20, Cscnl21, Cscnl22 and Cscnl23, Fig 1B). We screened a bacterial artificial chromosome (BAC) library of the resistant T. monococcum accession DV92 [22] using the closest markers, but sequencing of selected BACs showed no connection between proximal and distal groups (S1 Fig). We initiated a chromosome walk from the closest marker (CJ961291,0. 02 cM from the phenotype) in the proximal group (Fig 1C, BAC 205J7). Marker CJ961291 (RRM2) showed a recombination event with gene CNL1, which was completely linked to the phenotype, completing the proximal side of the physical map. After five cycles of library screening, BAC sequencing and marker development, we identified an additional recombination event between the phenotype and marker 179C18F7R7, which closed the distal end of the physical map. Sequencing the 405-kb candidate region (Fig 1D, blue area, S1 Fig red colored BACs, GenBank accession MG582649) revealed four complete NLR genes (henceforth, CNL1, CNL3, CNL5, and CNL6) and three NLR truncated genes with premature stop codons (henceforth, cnl2, cnl4, and cnl7). Multiple rearrangements were observed in this NLR cluster in T. aestivum cv. Chinese Spring (419 kb, Fig 1E, IWGSC RefSeq v1. 0) and T. turgidum ssp. dicoccoides Zavitan (435 kb, Fig 1F, Zavitan WEWSeq v1. 0) [23]. The two polyploid species included the complete gene CsCNL8 (ZaCNL8) that was absent in T. monococcum, whereas the T. monococcum region including cnl2, CNL3, cnl4 and CNL5 was missing in Chinese Spring and Zavitan (Fig 1E and 1F). NLR pseudogenes Cscnl10, Cscnl11 and Cscnl12 identified in CS were not detected in T. monococcum or Zavitan. The CS and Zavitan pseudogenes Cscnl1a (Zacnl1a) and Cscnl1b (Zacnl1b) were similar to T. monococcum CNL1 (~99% identity). Cscnl1a was interrupted by the insertion of a repetitive sequence in CS but not in Zavitan (Fig 1E and 1F). We sequenced the four NLR genes completely linked to the Sr21 phenotype (CNL1, CNL3, CNL5 and CNL6) from diploid accessions G3116 (Sr21, resistant) and PI 272557 (no-Sr21, susceptible). Using seven different pairs of gene-specific primers, we amplified CNL3 and CNL5 from DV92 but not from G3116 and PI 272557 (similar to CS and Zavitan, Fig 1E and 1F). Since G3116 carries the Sr21 resistant allele, the absence of these two genes suggested that CNL3 and CNL5 were unlikely candidate genes for Sr21. CNL6 was PCR-amplified in all three accessions, but G3116 showed a premature stop codon at position 742 (Q742*), suggesting that this gene was not Sr21. By contrast, CNL1 was PCR-amplified from both resistant accessions (DV92 and G3116) but not from Pgt susceptible accession PI 272557. Taken together, these results suggested that CNL1 was the best candidate gene for Sr21 among the NLR genes linked to Sr21. This hypothesis was further supported by expression data for these genes in transcriptome databases of DV92 and G3116 [24]. Among the four NLR genes completely linked to Sr21, we only detected transcripts for CNL1 in both DV92 and G3116 databases (Fig 1D). To test if CNL1 was required for Sr21 resistance, we mutagenized the Sr21 introgression lines in Chinese Spring (henceforth CSSr21) with ethyl methane sulfonate (EMS) and generated 1,151 M1 mutant plants. Among the 1,151 M2 mutant families screened with race BCCBC, we identified seven showing susceptible plants (M9, M66, M71, M271, M279, M287 and M306, Fig 2A and 2B). M3 seeds from the susceptible plants were also susceptible to Pgt race MCCFC (Fig 2C). Sequencing of CNL1 from the seven susceptible mutants (primers in S1 Table) revealed amino acid changes in five mutants and premature stop codons in two mutants (Fig 2A). Since the probability of a truncation or missense mutation in an annotated gene in a hexaploid wheat line mutagenized with 0. 8% EMS is roughly 0. 02 [25], the probability of detecting such changes in seven independent mutants by chance is < 1. 3×10−12. These results demonstrated that CNL1 is required for Sr21 resistance to Pgt. To test if CNL1 was also sufficient to confer resistance to Pgt, we generated thirteen independent transgenic events (T0Sr21) in the susceptible common wheat variety Fielder. Among the 13 T0 plants, we prioritized five that showed higher transcript levels of CNL1 than Fielder, which has a non-functional copy of CNL1 (S2 Fig). The T1 progenies from these five events showed segregation for resistance when challenged with Pgt race TTKSK (isolate 04KEN156/04). Some of the resistant transgenic plants showed even better levels of resistance than the CSSr21 positive control, which carries a Sr21 introgression from T. monococcum (Fig 3A). These results confirmed that CNL1 is sufficient to confer resistance to TTKSK. Taken together, the high-density map, the mutants and the transgenic results confirmed that CNL1 is Sr21. The observed segregation of resistant and susceptible T1 transgenic plants (Fig 3A) suggested that four of these five transgenic lines have more than one independent functional CNL1 insertions. Genotypes from roughly 50 T1 plants from each event showed significant departures from the expected 3: 1 segregation ratio (P < 0. 001, S2 Table), which suggested the presence of three to six copies of CNL1. This was validated by TaqMan copy number assays [26] (S2 Table). The larger copy number estimates obtained from the genotypic data than from the phenotypic data is likely explained by the presence of non-functional CNL1 insertions and/or by the presence of linked functional copies. On average, the five selected T1 transgenic events showed 2. 6- to 5. 7-fold higher CNL1 transcript levels than CSSr21 (S2 Table and Fig 3B), confirming the T0 results (S2 Fig). Copy number based on the CNL1 TaqMan assay was significantly correlated with average transcript levels in the five selected transgenics and CSSr21 (R = 0. 83, P = 0. 039). A similar correlation (R = 0. 81, P = 0. 0009) was observed between the T0 expression levels from all 13 transgenic lines and the copy number estimated by the TaqMan assay (S2 Fig). These results suggest that the differences in expression are driven, at least in part, by differences in CNL1 copy number. This was also reflected in the levels of resistance to TTKSK, where a negative correlation was observed between CNL1 copy number and average rust pustule size (R = -0. 92, P < 0. 0001, S3 Fig). In this experiment, we also compared the CNL1 transcript levels between the Sr21-resistant accessions G3116 (diploid) and CSSr21 (hexaploid) and found no significant differences (Fig 3B). We compared the sequences of the CNL1 transcripts (from the DV92 and G3116 transcriptome database [24]) with the corresponding genomic sequences and determined that the 4,872 bp coding sequence of CNL1 is divided in three exons that encode 1,624 amino acids. The predicted protein includes an N-terminal coiled-coil (CC) domain, a central nucleotide-binding (NB) site, and a leucine-rich repeat (LRR) region, typical of many NLR proteins. A nuclear localization signal (NLS) was predicted in Sr21 between amino acids 141 and 152 (cNLS Mapper http: //nls-mapper. iab. keio. ac. jp/cgi-bin/NLS_Mapper_form. cgi). Comparing different transcripts and genomic sequences, we identified a 210-bp 5’ untranslated region (UTR) and a 2,159-bp 3’ UTR. The 5’ UTR does not include any introns, whereas the 3’ UTR shows 2 to 4 introns depending on the alternative splice forms (S4 Fig). These results were confirmed by 5’ and 3’ rapid amplification of cDNA ends (5’ and 3’ RACE). We identified 10 CNL1 alternative splicing forms, which differ only in their 3’ UTR regions (S4 Fig). We detected five alternative splicing forms (CNL1-1 to CNL1-5) in the transcriptomes of DV92 and G3116 [24] and an additional five (CNL1-6 to CNL1-10) in the 3' RACE reactions. Since Sr21 resistance is modulated by temperature [20], we characterized the frequency of the different alternative splice forms in 3' RACE reactions from RNAs obtained from G3116 T. monococcum resistant plants grown at 24°C and 16°C. The plants at each temperature were further divided in mock-inoculated and inoculated with Pgt race BCCBC, and RNA samples were extracted six days after inoculation (80 clones per treatment were sequenced). χ2 tests showed no significant differences (P = 0. 81) in the frequencies of alternative splice forms in inoculated vs. mock-inoculated plants (averaged across temperatures) but detected significant differences between temperatures (P = 0. 002, averaged across inoculation treatments, S3 Table). The CNL1-1, CNL1-5 and CNL1-6 forms were more frequent at 24°C than at 16°C, and the opposite was observed for CNL1-2. We currently do not know the biological significance of these differences. We analyzed CNL1 transcript levels in the four temperature / inoculation combinations described above in resistant accessions G3116 (diploid) and CSSr21 (hexaploid). We took samples immediately after moving the plants inoculated with Pgt from a greenhouse (~20°C) to growth chambers at 16°C and 24°C (time 0 h, Fig 4). As expected, we found no significant differences in CNL1 transcript levels between temperatures or inoculations at the control sampling point (S4A Table). The CNL1 basal transcript levels relative to ACTIN were 14. 8% higher in CSSr21 than in G3116 (P = 0. 0089, S4A Table). For the samples collected at 1,3 and 6 days post inoculation (dpi), we performed a four-way ANOVA for CNL1 transcript levels including genotype, temperature, inoculation treatment and day after inoculation as factors. We found no significant differences between genotypes (diploid G3116 and hexaploid CSSr21), but detected significantly lower transcript levels in plants grown at 16°C than at 24°C (36. 5%, P < 0. 0001), and in Pgt inoculated plants relative to mock-inoculated plants (45. 8%, P < 0. 0001) (Fig 4, S4B Table). A smaller effect was detected among days (P = 0. 02), but no clear trend was observed. We used the same RNA samples collected 6 dpi from CSSr21 and G3116 described above and primers described in a previous study [16] to quantify the transcript levels of six pathogenesis-related (PR) genes (PR1, PR2, PR3, PR4, PR5 and PR9 = TaPERO). For all six PR genes, transcript levels were significantly higher (P < 0. 0001) in Pgt-inoculated plants than in mock-inoculated plants (S5 Table and S5 Fig). The overall differences in transcript levels between temperatures were not significant for some PR genes (PR1, PR4, PR9) but the interactions between temperature and inoculation were all highly significant in the combined ANOVA (P < 0. 001, S5 Table). These interactions are clear in S5 Fig, which shows lower PR transcript levels at 24°C than at 16°C in the mock-inoculated plants but significantly higher levels in the Pgt-inoculated plants at 24°C than at 16°C. To confirm that Sr21 was required for the coordinated upregulation of the six PR genes at 24°C, we compared the transcript levels of these six genes in the resistant hexaploid line CSSr21 and its derived susceptible sr21-mutant M9 (Fig 5). Plants from both genotypes were inoculated with race BCCBC or were mock inoculated. For all six PR genes, the differences between genotypes, inoculations and the interactions genotype x inoculation were highly significant (P < 0. 001) in two-way factorial ANOVAs. In all cases, a strong upregulation of all six PR genes was observed after inoculation with race BCCBC relative to mock inoculation only in the resistant genotype CSSr21 (Fig 5). This result confirmed that the coordinated upregulation of these six PR genes was triggered by Sr21. Sr21 showed higher levels of resistance to BCCBC when plants were grown at 24°C than when grown at 16°C (Figs 6A, 6B and S6). Similar differences were observed before for Sr21 resistance response to TTKSK at 20°C and 16°C [20]. These observations were confirmed in a three-way ANOVA for average pustule size 14 days post inoculation (dpi) (S6 Table). This analysis showed highly significant (P < 0. 0001) effects for ploidy level and genotype (presence or absence of Sr21), and a very strong interaction between temperature and genotype (P < 0. 0001). Plants having the Sr21 resistance gene showed smaller sporulation areas at high temperatures, whereas those without Sr21 showed significantly larger sporulation areas at high temperatures (Fig 6A and 6B). These opposite effects masked the main effect of temperature for race BCCBC (S6 Table). We also quantified the differences in BCCBC growth at five dpi by measuring the ratio of Pgt DNA relative to wheat DNA (Fig 6C and 6D) and average infection areas using microscopy and a fluorescent dye that stains the pathogen (Figs 6E, 6F and S7). Both methods showed highly significant (P < 0. 0001) differences between genotypes and between temperatures. As with the sporulation area, we also detected highly significant interactions between genotype and temperature for both methods (P < 0. 0001, S6 Table), which reflected the larger differences between genotypes at high than at low temperature (Fig 6C–6F). Average areas for sporulation at 14 dpi (TTKSK and BCCBC) and for pathogen growth (BCCBC determined by fluorescence) were larger in the Sr21-resistant hexaploid than in the resistant diploid plants, which agrees with a previous report [20]. Fluorescent images of Pgt growth in diploid and hexaploid wheat plants lacking Sr21 at high temperature show a diffuse network of hyphae at the borders of the infected areas, which seems to be expanding without host resistance. By contrast, in the presence of Sr21, the infected areas are smaller and their borders are denser (S7 Fig), which suggests that the expansion of the hyphae is facing opposition from the host. At the macroscopic level, a chlorotic halo was observed around the Pgt pustules in the Sr21 resistant reactions, which in some cases resulted in cell death. However, Pgt resistance conferred by Sr21 is mostly associated with a delay in the progression of the disease (partial resistance) rather than with a rapid hypersensitive reaction. We evaluated 114 T. monococcum and T. urartu accessions with Pgt races MCCFC and BCCBC and confirmed the presence of Sr21 in 44 of them and its absence in 70. We sequenced the complete CNL1 gene from these accessions using three pairs of primers (S1 Table and Fig 2A) and identified four susceptible haplotypes (S1 to S4) and five resistant haplotypes (R1 to R5, S8 Table and S8 Fig). The presence of Sr21 in the resistant diploid wheat accessions is based on inoculation with Pgt races TRTTF, TTKSK, TTTTF, QFCSC, and MCCFC (S7 Table). Of the 44 resistant accessions, 28 were classified as haplotype R1 (MG582649, equal to DV92), six as R2 (MG601519), one as R3 (MG601520), six as R4 (MG601521) and three as R5 (MG601522, S8 Table). The R1 haplotype differed from the other four by one (R2), three (R3), two (R4) and three (R5) amino acid changes, respectively (S8 Fig). Of the 30 T. monococcum susceptible accessions, 25 showed identical sequences and were classified as haplotype S1 (MG601523,90. 2% identical at the cDNA level to CNL1 haplotype R1). S1 is likely a non-functional gene since it carries a frame shift mutation. Surprisingly, polymorphisms between R1 and S1 were concentrated in the CC-NBS domains (87. 2% identity) whereas the LRR was more similar to CNL1 (99. 2% identity), which suggests a recombination or conversion event. A phylogenetic analysis including the DNA coding regions of the different NLR genes in the CNL1 region, and the closest paralogues in B. distachyon and H. vulgare showed that the CNL1 S1 haplotype is more closely related to cnl7 than to the cluster including the different CNL1 haplotypes (S9 Fig). All 40 T. urartu accessions showed haplotype S2 (MG601524), which was similar to R1 (99. 2% identical). This haplotype also includes three accessions classified as T. monococcum subsp. aegilopoides in the NSGC. However, analysis of 12 additional genes showed T. urartu haplotypes in these three accessions, suggesting that they are misclassified (we counted them as T. monococcum in the numbers reported above). The S2 haplotype from T. urartu, and the related haplotypes found in wild tetraploid Zavitan and hexaploid cultivars Chinese Spring and Fielder corresponds to the pseudogene Cscnl1b from Fig 1 and shares a 2-bp deletion at positions 3,255 and 3,256 (cDNA coordinates). When this shift mutation is manually corrected, these accessions share 20 amino acid polymorphisms compared to the R1 haplotype (S8 Fig). The cultivated tetraploid Kronos shares 14 of the 20 amino acid polymorphisms found in Chinese Spring, Fielder and Zavitan. Kronos does not have the 2-bp frame shift mutation, but has two different 1-bp deletions at positions 928 (a missing A) and 3,667 (a missing T). The susceptible haplotypes S3 (MG601525) and S4 (MG601526) were very similar to R1 (> 99. 9% identical) and included a single accession each (S8 Table). Three accessions from the Balkans, classified as S1 based on their CNL1 sequence (PI 355538, PI 362610 and PI 377668), showed a resistance reaction when inoculated with race MCCFC but were susceptible to BCCBC. Since Sr21 is resistant to both BCCBC and MCCFC, this result suggested that these three accessions carry a Pgt resistance gene different from Sr21. To develop a diagnostic marker for the Sr21 resistant haplotypes introgressed into polyploid wheat, we designed primers based on the C1228W diagnostic polymorphism and the T1500R and H1501S polymorphisms that separate Sr21 resistant and susceptible haplotypes. PCR amplification with primers Sr21TRYF5R5 (S1 Table) at an annealing temperature of 56°C generates a 951-bp fragment when the T. monococcum haplotypes are present and no amplification in T. urartu, tetraploid or hexaploid wheat (S10 Fig). Treatment of the amplified PCR products with restriction enzyme NsiI generated two bands of 836 and 115 bp for the T. monococcum accessions carrying the Sr21 susceptible haplotypes and a single 951-bp band for the accessions carrying the resistant haplotypes (S10 Fig).
  The T. monococcum genomic region encompassing the Sr21 resistance gene includes four NLR genes and three pseudogenes (Fig 1). The clustering of NLR genes facilitates the generation of novel variants through recombination and conversion events, increasing genetic variability in resistance genes [27]. Ectopic recombination events can generate deletions and duplications, and these were frequent in the Sr21 region. We detected several rearrangements between T. monococcum, hexaploid wheat Chinese Spring and wild tetraploid wheat Zavitan [23] (S1 Fig) that diverged less than one million years ago [28]. A T. monococcum region of more than 150-kb including cnl2, CNL3, cnl4 and CNL5 was not detected in any of the polyploid species. Similarly, a large region in Chinese Spring between CsCNL8 and Cscnl12 was deleted in T. monococcum and part of it in Zavitan. Finally, a region including CNL6 and cnl7 was detected in diploid and tetraploid wheat but was absent in hexaploid wheat. These large differences suggest that this NLR cluster has experienced rapid evolutionary changes. The presence of four related NLR genes and pseudogenes in the colinear region of Brachypodium distachyon chromosome 5 (Figs 1 and S9) suggests that this NLR cluster has a long evolutionary history that extends beyond the divergence between the Triticum and Brachypodium lineages more than 30 million years ago [29]. When we characterized Sr21 expression, we detected multiple alternative splicing forms of CNL1, which differed in the intron structure of the 3’ UTR region (S4 Fig). Alternative splicing forms have been identified in several CC-NBS-LRR genes, including Pi-ta in rice [30], Mla in barley [31], and Lr10 [32] in wheat. Complex UTR regions with multiple introns have been also described for other wheat NLR genes involved in resistance to Pgt including Sr35 [12] and Sr13 [16]. In Sr21, we observed differences in the frequencies of the main alternative splicing forms with temperature, but the role of these differences is currently unknown. Results presented here and in a previous study [20] indicate that Sr21 is less effective when present in a hexaploid background than in a diploid background. Since the hexaploid Sr21 gene was introgressed directly from T. monococcum [21], the encoded proteins are expected to be identical. In addition, we found no significant differences between diploid and hexaploid wheat in Sr21 transcript levels. Therefore, differences downstream of Sr21 transcription are likely responsible for the reduced resistance conferred by Sr21 in hexaploid than in diploid wheat. Reduced stability of the Sr21 protein in hexaploid wheat or reduced compatibility between the T. monococcum Sr21 protein and some of its downstream T. aestivum protein interactors are possible explanations. Both hypotheses can explain the stronger upregulation of downstream PR genes observed in diploid than in hexaploid Sr21-resistant wheat accessions (1. 5 fold for PR2 to 14. 6 fold for PR9, S5 Table). In both species, the coordinated upregulation of PR genes was observed only in the presence of the pathogen and the resistance gene, suggesting that the Sr21 protein needs to be activated through interactions with a Pgt effector or a wheat protein modified by Pgt. The stronger resistance response observed at 24°C suggests that temperature modulates some of the involved processes. Elevated growth temperatures have been reported to affect plant resistance to diseases by reducing steady-state levels of resistance protein at high temperatures [33], affecting temperature-sensing NB-LRR proteins [34], or affecting salicylic acid (SA) regulation [35,36]. A similar inhibition of the resistance response by high temperatures has been reported for wheat Pgt resistance genes Sr6, Sr10, Sr15, and Sr17. By contrast, Sr13- [16] and Sr21-mediated Pgt resistances are more effective at higher temperatures. Both genes also show a coordinated upregulation of the same PR genes at high temperatures, which suggests that they may share some common mechanisms. Although the mechanisms by which Sr21 and Sr13 [16] coordinate the upregulation of PR genes are currently unknown, information from other species suggests that the NPR1 pathway or WRKY transcription factors may be involved. Previous studies in Arabidopsis have shown that NPR1 interactions with TGA transcription factors play an important role in the regulation of several PR genes [37,38]. This was also observed in barley, in which overexpression of a conserved protein from the stripe rust pathogen that competes with TGA transcription factors for the binding with NPR1, reduced the induction of several PR genes in a leaf region adjacent to a bacterial infection [39]. WRKY transcription factors are also interesting candidates because the promoter of several PR genes contain W-box elements recognized by these proteins [40,41]. In addition, NLR proteins from barley (MLA) and rice (Pb1) have been shown to interact with WRKY transcription factors to regulate their defense responses [42,43]. It would be interesting to determine if Sr21 or Sr13 can interact with NPR1, WRKY or other transcription factors to coordinate the upregulation of wheat PR genes. The results presented here provide useful information for the utilization of Sr21 in agriculture. Since Sr21 is susceptible to some Pgt races, it needs to be deployed in combination with other Pgt resistance genes or in transgenic cassettes including multiple resistance genes. Given the better Ug99 resistance levels observed in transgenic plants carrying more than one active copy of Sr21, it might be valuable to include at least two copies of Sr21 in the transgenic cassettes. It might be also advisable to avoid combining Sr21 and Sr13, since both genes seem to operate by a similar mechanism involving the activation of multiple PR genes at high temperature. Even if Sr21 and Sr13 recognize different effectors, the pathogen could bypass both resistance genes simultaneously by attacking a single target if the two genes share a common downstream signaling pathway. Combining genes that operate by different mechanisms may reduce the probability that a single change in the pathogen can defeat multiple pyramided genes [44]. Sr21 confers only partial resistance to Ug99, but this might be useful in programs that aim to combine multiple partial resistance genes and avoid major all-stage resistance genes, a strategy that has been proposed to increase the durability of wheat resistance to rusts [45]. The deployment of Sr21 in commercial tetraploid or hexaploid commercial varieties can be accelerated by the diagnostic marker developed in this study (S10 Fig).
  A total of 7,168 recombinant gametes from two segregating populations were used to construct a high-resolution genetic map of Sr21. These populations included 734 F2 plants from population PI 272557 × DV92 and 2,850 from population PI 272557 × G3116. Plants with informative recombination events were challenged with races MCCFC (isolate 59KS19) and TTKSK (isolate 04KEN156/04) at the USDA-ARS Cereal Disease Laboratory and with race BCCBC (isolate 09CA115-2) at the University of California, Davis (UCD). Assays of response to race TTKSK were performed at 25°C during the day and 22°C during the night with a 16 h photoperiod (Fig 3) and those for BCCBC at 16°C (low temperature) and 24°C (high temperature). Procedures for inoculation and statistical analyses of infection types were reported previously [20]. A Bacterial Artificial Chromosome (BAC) library from the resistant parent DV92 [22] was used to generate the physical map by chromosome walking. DNAs from the selected BACs were extracted using QIAGEN Large-Construct Kit. BACs were fingerprinted using restriction enzyme HindIII, were sequenced using a combination of Illumina Hi Seq2500 at the Beijing Genomic Institute (Sacramento, CA, USA) and WideSeq at Purdue Genomics Core Facility (https: //www. purdue. edu/hla/sites/genomics/wideseq-2/), and were assembled using Galaxy [46,47]. We identified and annotated the repetitive elements in the Sr21 region using the Triticeae Repeat Sequence Database (http: //wheat. pw. usda. gov/ITMI/Repeats/blastrepeats3. html) and the genes using BLASTN / BLASTX searches in GenBank (http: //www. ncbi. nlm. nih. gov/). These three websites were last accessed February 26,2018. Mutant lines were generated by treating 10,000 seeds from the hexaploid wheat line CSSr21 (Sr21 resistance haplotype R1) with 0. 8% ethyl methane sulphonate (EMS). Seeds from 1,151 independent M1 mutants were harvested, and 25 M2 seeds per family were planted and inoculated with Pgt race BCCBC (isolate 09CA115-2) at UCD. Twenty-five M3 seeds from susceptible M2 plants were retested with race MCCFC (isolate 59KS19) at the USDA-ARS Cereal Disease Laboratory. Rapid amplification of cDNA ends (RACE) was performed using total RNA extracted from leaves of resistant parent DV92. Both 5' RACE and 3' RACE were performed using the FirstChoice RLM-RACE Kit (Invitrogen) following the manufacturer’s instruction. The PCR products from Nested PCR amplifications were cloned using the TA cloning kit (Invitrogen). A 10,463-bp genomic DNA fragment including Sr21 was amplified from DV92 BAC clone 205J7 using Phusion High-Fidelity DNA Polymerase (New England BioLabs Inc.). This fragment, including the complete Sr21 (5,208 bp) coding region and introns, 2,772 bp upstream from the start codon, and 2,483 bp downstream from the stop codon, was cloned into the binary vector pLC41Hm. The resulting plasmid pLC41HmSr21 was transformed into Agrobacterium strain EHA105, and was transformed into the Ug99-susceptible wheat variety Fielder (CNL1 haplotype S2) at the UC Davis transformation facility (http: //ucdptf. ucdavis. edu/). Primers HptmikiF/R developed from the hygromycin resistance gene and primers S21CNL1F5R5 and Sr21TRYF5R5 developed from CNL1 were used to confirm the presence of transgene (S1 Table). A TaqMan Copy Number Assay was used to estimate the number of copies inserted in every transgenic event as described before [16]. Sequences from the 3' RACE described above and from the transcriptome databases of DV92 and G3116 [24] revealed the presence of several alternative splicing forms at the 3’UTR region of Sr21. To determine the number of alternative splicing variants, we first extracted total RNAs from G3116 T. monococcum plants grown at 16°C and 24°C six days after inoculation. We used these RNAs for 3’RACE reactions and cloned the PCR products using the TA cloning kit (Invitrogen). Eighty colonies from every 3' RACE reaction were PCR-amplified and sequenced using the Sanger method. Total RNA was extracted from leaves of Sr21-resistant diploid T. monococcum ssp. aegilopoides accession G3116 and hexaploid CSSr21 using Spectrum Plant Total RNA Kit (Sigma-Aldrich). First strand cDNA was synthesized from 1 µg of total RNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Samples were collected from plants grown under the four treatments resulting from the combination of two temperatures (16°C or 24°C) and two inoculation treatments (Pgt race BCCBC or mock inoculation). Samples were collected immediately after inoculation (0 h) and 1,3 and 6 days post inoculation (dpi). We quantified Sr21 expression using primers Sr21qRTF1R1 (S1 Table). The same cDNA samples were used to quantify the transcript levels of six pathogenesis-related (PR) genes (PR1, PR2, PR3, PR4, PR5 and PR9 = TaPERO) using primers described before [16]. We performed the qRT-PCR reactions on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems) using Fast SYBR GREEN Master Mix. Transcript levels were expressed as fold-ACTIN levels (the number of molecules in the target / the number of ACTIN molecules) using the 2ΔCT method as described before [48]. We calculated the significance of the differences in expression levels using factorial ANOVAs and the SAS program version 9. 4. From a previous study [49], we selected 44 T. monococcum accessions (28 cultivated and 16 wild type) resistant to races MCCFC and TTKSK and susceptible to races TTTTF, TRTTF and QFCSC, which were previously postulated to carry only Sr21 (S7 Table). We also selected 30 accessions of T. monococcum and 40 from T. urartu that were susceptible to all five races (S7 Table). Seeds were obtained from the U. S. Department of Agriculture National Small Grains Collection (NSGC). All T. monococcum and T. urartu accessions were re-evaluated with races MCCFC (isolate 59KS19) and BCCBC (isolate 09CA115-2) (at 24°C) for this study. We also confirmed the absence of Sr35 with the diagnostic marker developed from the cloned Sr35 gene [12]. Seeds from three T. monococcum accessions showed heterogeneous resistance reactions to MCCFC (PI 427971, PI 119422 and PI 225164) and were listed twice in S8 Table (as–S and–R) resulting in a total of 74 T. monococcum accessions. The sequenced genomes of diploid wheat T. urartu (http: //plants. ensembl. org/Triticum_urartu/Info/Index), tetraploid wheat Zavitan (https: //wheat. pw. usda. gov/GG3/wildemmer), and hexaploid wheat Chinese Spring RefSeq v1 (https: //urgi. versailles. inra. fr/blast_iwgsc/blast. php) were used to detect the corresponding CNL1 alleles.
summary: Wheat stem rust is a devastating disease that is threatening global wheat production. The emergence of new virulent races of this pathogen in Africa, including the Ug99 race group, has prompted global efforts to find effective resistance genes. We report here the identification of stem rust resistance gene Sr21 that is effective against the Ug99 race group. We developed a diagnostic marker to accelerate its deployment in wheat breeding programs and demonstrated that the introduction of two Sr21 copies in transgenic wheat results in high levels of resistance. An unusual characteristic of Sr21 is its increased resistance to stem rust at high temperatures. We show here that this is associated with the ability of Sr21 to coordinate the upregulation of multiple pathogenesis related genes at high temperatures. These genes slow down the growth of the pathogen and result in the characteristic Sr21 intermediate resistance reaction at high temperatures. A better understanding of this temperature dependent resistance mechanism will be useful for controlling the rust pathogens in our changing environments.
keywords: biotechnology
  departures from diploidy
  genetic mapping
  alternative splicing
  plant science
  chromosome mapping
  genetically modified plants
  molecular biology techniques
  plants
  hexaploidy
  genetic engineering
  research and analysis methods
  grasses
  genetically modified organisms
  gene mapping
  artificial gene amplification and extension
  gene expression
  wheat
  polyploidy
  molecular biology
  biochemistry
  rna
  haplotypes
  eukaryota
  rna processing
  nucleic acids
  polymerase chain reaction
  heredity
  genetics
  biology and life sciences
  plant biotechnology
  organisms
title: Identification and characterization of wheat stem rust resistance gene Sr21 effective against the Ug99 race group at high temperature