article: Current methods for the identification of putatively co-regulated genes directly from gene expression time profiles are based on the similarity of the time profile. Such association metrics, despite their central role in gene network inference and machine learning, have largely ignored the impact of dynamics or variation in mRNA stability. Here we introduce a simple, but powerful, new similarity metric called lead-lag R2 that successfully accounts for the properties of gene dynamics, including varying mRNA degradation and delays. Using yeast cell-cycle time-series gene expression data, we demonstrate that the predictive power of lead-lag R2 for the identification of co-regulated genes is significantly higher than that of standard similarity measures, thus allowing the selection of a large number of entirely new putatively co-regulated genes. Furthermore, the lead-lag metric can also be used to uncover the relationship between gene expression time-series and the dynamics of formation of multiple protein complexes. Remarkably, we found a high lead-lag R2 value among genes coding for a transient complex.
  Gene expression is a highly regulated process composed of two fundamental biological events: synthesis and degradation. Transcription regulation is achieved by modulating the frequency of transcription initiation and, although the most studied, this event represents just the first of the many complex stages leading to a mature mRNA. Recent experimental work is beginning to shed light on the complex architecture underlying mRNA degradation pathways by identifying the factors and enzymes involved. Therefore, it is now widely accepted that mRNA decay contribution to the control of gene expression is not simply a biological waste-disposal system, but a key player for the temporal coordination of cellular functions. Moreover, a number of highly complex and sophisticated specific mechanisms have been identified [1]. Such mechanisms include the interaction with mRNA binding proteins [2] and the nonsense-mediated mRNA decay pathway [3], both able to affect the accumulation of hundreds of transcripts. Recent technologies, such as microarrays, are able to provide measurements of mRNA abundance over time under different experimental conditions. In order to decipher the intricate regulatory network underlying the highly coordinate cell behavior, effective computational methods have been developed to take advantage of gene expression data. The basic idea underlying such methods stems from the experimental observation that genes are organized in groups showing similar time profiles [4] (called “clusters”). These groups often share some common biological features, such as the same cellular function or the presence of a common motif at their promoter regions [5] where transcription factors (TFs) can bind and possibly turn them on or off in a coordinated manner, when needed. For this reason, it is now widely accepted that co-expression is a good indication for co-regulation [6]–[8], meaning that whenever two genes display similar time profiles it is likely that they are both targets of the same transcription factor (s). The search for co-regulated genes depends on association metrics used by clustering algorithms [5], [9], [10] and gene network inference algorithms [11]–[13]. Therefore, measuring the degree of co-expression of genes is a fundamental step for data analysis, and in fact, many similarity measures have been proposed in the literature [14]. Among those available to quantitatively measure simultaneous expression, we will refer to the usual R2 value obtained from a linear regression model between two given gene expression time profiles denoted by mA (t) and mB (t). Their co-varying degree is therefore measured as the fraction of the total variance explained by the regression mA (t) = c1mB (t) +c2. Such coefficient, indicated in this paper as the simultaneous R2 of the corresponding gene pair, is the square of the Pearson correlation and takes values between 0 and 1. In order to infer the gene regulatory network, several laboratories have combined microarray data with protein-DNA interaction data, taking advantage of ChIP-on-chip experiments [15]. Such studies have shown that the same transcription factor (or combinations of) may target genes with very different expression time profiles, even in the same experimental condition. For example, the targets of the yeast cell cycle transcriptional regulators MBF/SBF display expression peak times that span from early G1 to late S. Moreover, delays have been recently observed between putatively co-regulated genes [16], [17]. One fundamental biological mechanism underlying such temporal spread is certainly combinatorial regulation of transcription factors. In fact, various TFs can modulate target response by cooperating or competing for DNA binding. Consequently, new computational techniques have recently appeared in the literature to tackle this problem [18]–[25]. However, combinatorial regulation is not the only mechanism responsible for peak time delay, as other regulation layers are active throughout transcript life and impact its abundance over time. One such additional regulation layers is certainly the post-transcriptional one, that is the stability properties of transcripts that may specifically contribute to the determination of their timing and amount during cell response to various internal and/or external signals. Strikingly, recent genome-wide measurement of the yeast transcripts half-lives [26], [27] has shown functional specificity in mRNA decay. Together, these results pointed to a general relationship between physiological function and mRNA decay rate thus providing strong evidence that precise control of mRNA turnover is a fundamental feature of gene expression programs in yeast [26] and in many other organisms. Here we focus on the development of a novel computational tool aiming to uncover co-regulated genes through transcriptional and post-transcriptional regulatory mechanisms. To this purpose, starting from the computational approach developed by Farina et al. [28], we introduce a new relationship between gene pairs, called lead-lag relationship. The term “lead-lag” has been taken from the field of control systems engineering where the same relationship holds between the input and the output of the so called “lead-lag compensator”, which is the fundamental building block for the design of automatic control systems [29]. In a biological perspective, the lead-lag relationship should be referred to genes under a common regulatory signal (“input”) involved in the same biological function (“output”) as, for example, in the dynamic multi sub-units complex formation [30], [31]. Using yeast cell-cycle time-series gene expression data, we demonstrate that this new similarity metric is able to capture the dynamics of gene expression, including varying mRNA stability and delays. Thus, the predictive power of lead-lag R2 for the identification of co-regulated genes is significantly higher than that of standard similarity measures, allowing the selection of a large number of entirely new putatively co-regulated genes. Furthermore, the lead-lag metric can also be used to uncover the relationship between gene espression time-series and the formation of protein complexes.
  To clarify the specific features of gene regulation at the mRNA stability level, it is worth thinking of the case when two genes are turned on at the same time by the same transcriptional signal, and the newly synthesized transcripts of both genes are degraded at the same rate. Consequently, differences in their gene expression profile will be determined only by the response of the two genes to the transcriptional signal (i. e. different affinities of the transcription factor to promoter regions). A computer simulation of this situation is depicted in Figure 1A where two genes are expressed following a first-order kinetics (see Text S1. for details on the equations used for the simulation). The transcription is turned on at the same time for both genes but with a different rate: the first gene is transcribed more rapidly than the second one (Figure 1Aa). Their degradation rate is the same (Figure 1Ab) and therefore the two gene expression profiles differ only for the magnitude of the response, whereas preserving the shape of the curve (Figure 1Ac). In this case, the normalized time profiles are identical (Figure 1Ad) and therefore the simultaneous R2 is maximal (R2 = 1). Indeed, the “converse” situation is very different. Figure 1Ca–b illustrates the case in which the two genes are transcribed at the same rate while their degradation decreases at the same time but with a different rate. The two profiles do not have the same shape (Figure 1Cc). As a consequence, the corresponding simultaneous R2 will not be maximal (R2<1) as can be seen from differences in the normalized profiles (Figure 1Cd). Such considerations illustrate that the impact of stability regulation on time profiles is quantitatively and – most importantly – qualitatively different from that of transcription regulation. It is therefore not surprising that specific systems biology computational tools have begun to appear in the literature [28], [32]. The different impact of mRNA stability regulation versus transcription regulation results from the fact that the rate of mRNA degradation is proportional to the substrate concentration but the rate of production is not [33]. Such behaviour is reasonably well captured by a first order rate equation. In fact, messengers half-lives are experimentally measured usually by fitting a single exponential decay function to the time profiles observed after transcriptional shut-off [26]. Another important issue is that the differences of transcription rate regulation with respect to degradation rate regulation cannot be clearly seen by simply looking at the long term behavior of the response, i. e. at steady state values. In fact, the final amount of mRNA upon a prolonged regulatory signal equals the ratio transcription rate/degradation rate so that, from this perspective, a N-fold increase of transcription rate is equivalent to a N-fold decrease in degradation rate (and viceversa). An example of such behavior can be seen by comparing Figure 1Ac with Figure 1Cc: the steady state values are the same in both cases but the overall shape of the response (its “dynamics”) is very different. Such “loss of correlation” phenomenon due to differential stability regulation can be further understood by considering a time varying rates, resulting in a transient mRNA time profile, as shown in Figure 1Ba–d and 1Da–d. Again, an increase in the rate of transcription results in an increased response displaying a highly correlated temporal profile (Figure 1Bd), whereas a decrease in the degradation rate results in a low correlated temporal profile with a shift in peak time, as shown by Figure 1Da–d. It is plain that, by combining time varying transcription and degradation rates, a large variety of dynamic time patterns may be generated. It is important to note that peak timing regulation may also stem from time delays, as shown in Figure 2B, which can be generated by different biological mechanisms such as transcriptional combinatorial regulation, cascade regulations [34], feedforward motifs and single input motifs [35]. Time delays in gene expression data have been studied using delay correlation analysis [16], [36], [37]. The scenario depicted above naturally leads to the possibility that co-regulation may involve both the transcriptional and post-transcriptional machinery. Therefore, a large variety of temporal profiles can be obtained by combining any of those shown in Figure 2. In this paper we consider a novel relationship between gene expression time profiles which includes also the possible presence of mRNA stability variations as a further mechanism to modulate transcript abundance over time. Such new coordinated relationship will be called lead-lag relationship. Such terminology is borrowed from the field of system and control engineering where it refers to the basic building block for the realization of a regulatory device able to provide optimal properties to a given process and called “lead-lag compensator” [29]. In order to identify lead-lag relationships, we propose a quantitative measure between gene expression time profiles, called lead-lag R2, able to incorporate in a single parameter such relationship and consequently potentially enhancing the predictive power of gene expression analysis for the identification of putatively co-regulated genes. In fact, we aim to study here the possibility that an high lead-lag R2 between expression time profiles of two given genes is a good indication for the presence of a common regulation mechanism. The lead-lag R2 is quantitatively defined by a linear multiple regression model among the two given gene expression time profiles mA (t) and mB (t) and the area under curve until time t (i. e. their time integral over time): and measured by the lead-lag R2, that is the fraction of the total variance explained by the above multiple regression model. Such coefficient is computed directly from at least 6 time points of gene expression data and takes values between 0 and 1. The rationale behind such new relationship stems from a simple mathematical model conceived to capture, from gene expression time series data, those genes which are co-regulated at the transcriptional level having an equal or different mRNA stability. It is worth noting that the simultaneous relationship is also a particular lead-lag relationship (just set c2 = c3 = c4 = 0) so that the magnitude of the lead-lag R2 is always larger or equal than that of the simultaneous R2. In the following we will show that the magnitude of the increase from simultaneous R2 to lead-lag R2 is specific for each gene pair and that it is statistically correlated both to the presence of a common transcriptional signal and to differences between the half-lives. More details of the lead-lag R2 and its numerical computation are given in the Materials and Methods section. The mathematical model used to define the lead-lag R2 is based on the assumption that co-regulated genes have the same transcriptional signal (promoter activity) and equal or different transcript stabilities. Consequently, we postulated that two given genes showing a lead-lag relationship (namely, with high lead-lag R2 values) are likely to be regulated by common transcription factors. To test this hypothesis, we selected a list of 1159 genes indicated as cell-cycle regulated in at least one out of six yeast genome-wide studies [38]. We then used a large integrated dataset of yeast cell-cycle data generated by three independent groups using different synchronization methods and composed of 7 datasets (13 cell cycles for each gene, see Materials and Methods for details). We considered as “gold standard” the transcriptional regulatory network recently published by MacIsaac and collegues [15]. Such reconstructed network is very reliable since the authors combined complementary strategies to improve the ability to identify the specificity of transcriptional regulators from genome-wide chromatin immunoprecipitation data. The Mc Isaac et al. dataset consists of a list of targets for 203 TFs using different conservative criteria. Among those available 203 TFs, we selected a p-value for binding of 0. 001 obtaining a list of 3107 genes, containing 660 of the genes in the list of the cell cycle regulated ones. We then choose the 10 TFs widely recognized as having a fundamental role during the cell cycle [39]: SWI4, SWI6, MBP1, NDD1, FKH1, FKH2, MCM1, ACE2, SWI5 and YOX1. Using this data, we could assess the effectiveness of our approach by computing true and false positive rates and ROC curves. To this end, we evaluated the lead-lag R2 for each gene pair in the dataset (N (N−1) /2 pairs, N = 660) and considered as putatively co-regulated those pairs whose R2 values were over a threshold thigh and, as putatively non co-regulated, those pairs whose R2 values were below a threshold tlow. Gene pairs with scores between thresholds were not considered. In order to construct a ROC curve we used varying thresholds: as an upper threshold thigh for co-regulation we selected the value corresponding to percentiles p ranging from 50th to 90th with a step of 10 and, as a lower threshold tlow for non-coregulation, we selected the value corresponding to the “symmetric” percentile 100−p. For each threshold we could compute true positives, true negatives, false positives, false negatives and therefore construct a ROC curve (Figure 3A, green plots) where all the R2 values have been averaged over the 7 datasets. The average dataset has been constructed by computing the R2 values for each cycle and for each dataset, for a total amount of 13 cycles. The mean R2 value for each genes pair was obtained by computing the mean of the 13 available values. In case of missing data in the original dataset, computation of the mean R2 value was performed only when at least 8 out of 13 cycles were available. Each class of putatively co-regulated gene pairs was obtained by selecting those pairs exceeding the upper thresholds corresponding to the percentiles from 50th to 90th with a step of 10 of the R2 distribution. Therefore, true positives are those pairs of the class having at least one common transcription factor according to the Mc Isaac et al. dataset (p-value for binding<0. 001), whereas false positives are those pairs in the class without a common transcription factor (p-value for binding>0. 001). Analogously, true negatives and false negatives were computed within the class of gene pairs having the lower thresholds corresponding to the percentiles from 50th to 10th with a step of −10 of the R2 distribution. To evaluate the performance of predictions obtained with the lead-lag R2 we repeated the same analysis using the simultaneous R2 as a similarity measure between two given genes (Figure 3A blue plots). The results clearly show that the lead-lag R2 certainly outperforms standard analysis based only on simultaneous relationships, increasing the true positive rate from 80% to 95%. The fraction of false positives also slightly decrease but remains relatively high possibly due to the fact that we have considered highly conservative criteria and selected the targets of only those 10 transcription factors having a major regulatory role during the cell cycle. It is worth noting that the performances on the average dataset are much better than the average of all performances (see Figure 3B) thus showing that an integrative approach using multiple independent datasets is always the best choice, whenever applicable. Moreover, given the large number of datasets considered, we can also conclude that the results obtained are largely independent of the noise and the stress response induced by the synchronization methods. Finally, we note that the above results remain valid even if we consider the transcriptional network presented by McIsaac et al. [15] using different selection criteria for DNA binding (see Text S1). The peculiarity of the lead-lag relationship between two given genes relies on the presence of a common regulatory signal driving the expression of transcripts with equal or different mRNA half-lives. For this reason, we investigated whether co-regulated gene pairs having an high lead-lag R2 values are significantly enriched with differential transcript' s stabilities. Half-life values are not available during the cell cycle and in the same experimental conditions used for establishing cell synchronization. Nevertheless, genome-wide half-lives data for un-synchronized cells were published recently by Wang et al. [26]. Using DNA microarrays, the authors precisely measured the decay of each yeast mRNA in YPD medium, after thermal inactivation of a temperature-sensitive RNA polymerase II. Such half-life measurements were not obtained during the cell cycle, so that we do not expect an exact agreement with the actual ones. Nevertheless, by considering a large number of gene pairs (16740) it appears reasonable that, on average, the half-life ratios between gene pairs may not vary significantly. Therefore, we used such available data for a statistical evaluation of the presence of gene pairs with high lead-lag R2 values with respect to the simultaneous R2 among those co-regulated pairs having large half-life ratios. To this end, we considered all possible gene pairs having, at least, one common transcription factor according to the MacIsaac et al. dataset [15] using a p-value for binding less that 0. 001 and considered five half-life ratio bins: less than 2-fold, from 2-fold to 3-fold, from 3-fold to 4-fold, from 4-fold to 5-fold and more than 5-fold. We computed the simultaneous R2 and also the difference between the lead-lag R2 and simultaneous R2 for all the gene pairs in each of the half-life bins. Such difference is used in order to select that part of the lead-lag R2 value which is not due to the simultaneous espression of the gene pair. Therefore, we got a distribution of values for each half-life ratio bin and computed the corresponding mean value and standard deviation. Figure 4 shows the results of the above described computation. Figure 4A makes clear that the highest and the lowest half-life bin display very different lead-lag minus simultaneous R2 mean values. To further support this feature, we performed a t test and found that the increase of the mean value of the distribution in the first and the last bin of the lead-lag R2 minus the simultaneous R2 is indeed significant (95% confidence level, p-value 10−9). The simultaneous R2 also shows a mildly significant decrease (95% confidence level, p-value 0. 03) of the mean values between the first and the last bin. To further evaluate the statistical significance of this analysis we computed the Z-score corresponding to 100000 randomizations of the half-life measurements. The results are shown in the scatterplot of Figure 4B and they provide computational evidence that the lead-lag R2 of gene pairs is statistically correlated to their half-life ratios. In fact, a high positive Z-score (about 5) corresponds to the highest half-life ratio bin and a negative Z-score (about −5) corresponds to the first half-life ratio bin. On the other hand, Z scores for the simultaneous R2 are all within the values −3 and 3 and therefore the observed difference of the mean values between the first and the last bin is not significantly affected by the randomizations. This scenario is consistent with the biological process underlying the mathematical model used to define the lead-lag R2 thus showing that our analysis well captures the effects of post-transcriptional control on gene expression time profiles during the cell-cycle. The results presented so far have clearly shown that lead-lag correlation analysis outperforms the usual simultaneous correlation analysis (squared Pearson coefficient) for the prediction of co-regulation, i. e. the presence of a common transcription factor, from gene expression time profiles. As previously discussed, truly co-regulated genes do often display large differences of gene expression time profiles, e. g. peak shifts, delays or other kinds of nonlinear relationships. In this paragraph, we consider other similarity measures relevant to the analysis of gene expression data and compare their performances with those obtained using the lead-lag R2. In particular, we used 5 similarity measures other than the lead-lag: Spearman' s rank, Kendall' s tau, cosine, dynamic time-warped and time-delayed correlation, all squared to capture inverted relationships also. Spearman' s rank, Kendall' s tau and cosine correlation are the most common choices for the analysis of gene expression data in the presence of nonlinear relationships between time series, but they do not take into account the time ordering of data. By contrast, time-warped and time-delayed correlation have been specifically developed to analyze gene expression time profiles. The time-delayed correlation analysis has been proposed by Schmitt et al. [37] where, for any genes pair, a R2 value is obtained by selecting the highest simultaneous R2 over all admissible time delays between profiles. The dynamic time-warped correlation has been recently used by Aach and Church [40] and Hermans and Tsiporkova [41] for the alignment of gene expression time series obtained in experiments using different cell synchronization methods. These two works are both based, for gene-to-gene comparisons, on the Dynamic Time Warping (DTW) algorithm developed by Sankoff and Kruskal [42]. Accordingly, we defined a time-warped R2 by selecting the highest simultaneous R2 over all the possible time warped paths. For any similarity measure, we performed the same analysis reported in a previous section using the same data, and the results are shown in Figure 5 where sensitivity (panel A) and specificity (panel B) are reported for each threshold. First of all, the cosine correlation analysis produces the poorest performances, very close to a random choice, and therefore such similarity measure is not reported in Figure 5. On both panels we note that simultaneous, Spearman' s rank and Kendall' s tau provide comparable results which are clearly below the performances of the group of methods which take into account the time ordering of data. In this group the lead-lag correlation analysis shows the best performances, both in terms of sensitivity and specificity. In this section we present some examples of “typical” lead-lag relationships using the most recent yeast cell cycle data [43] and discuss their biological relevance. The complete list of gene pairs exceeding the 95th percentile of the distribution for each of the R2 values considered in this paper is provided in the supporting information file Text S1. The expression of genes in the cell is to a large extent controlled at the level of mRNA accumulation. One key point in the analysis of gene expression dynamics is that mRNA abundance is determined by two regulated processes: transcription and degradation both specifically affecting transcript levels. Computational analysis of genome-wide expression time series has shown that clusters of co-expressed (i. e. simultaneously correlated) profiles often provide clues for the presence of common transcription factors regulating both genes. Such computational analysis (known as “clustering”) is very useful since it allows the prediction of the underlying regulatory actions based exclusively on the available gene expression data obtained from a given experiment. The rationale behind such belief is a sort of a “guilty by association” approach: genes' products appearing and disappearing at the same time are likely to have some common transcriptional regulation. Nevertheless, it may well be the case that the same transcriptional signal regulating two (or more) genes may yield quite different outcomes on each transcript. In fact, a number of biological events following transcription may selectively affect cytoplasmic mRNA abundance, such as, for example, the activity of the enzymatic machinery involved in mRNA processing and degradation. In order to address this issue, we provided a novel computational methodology that, based exclusively on the available gene expression data, is able to effectively predict co-regulation even with variation in the dynamic response due to mRNA stability differences. Moreover, our approach also captures the relation of simultaneous or time shifted co-expression so that it provides a single integrative general index – the lead-lag R2−able to uncover the presence of a common regulatory signal underlying gene expression time dynamics also at the post-transcriptional level. In order to test the validity of our approach on real data, we used yeast genome-wide cell-cycle expression time series obtained by several independent groups using different synchronization methods. In fact, by doing so, we could integrate the available cell cycle data and obtain a much more reliable aggregated dataset. We considered those gene pairs with the highest lead-lag R2 values and found the prediction for the presence of a common transcription factor to be highly consistent with protein-DNA binding data (ChIP experiments). Our results clearly indicate that co-regulation is not generally equivalent to simultaneous expression. We believe that the same analysis can be successfully used to predict post-transcriptional regulation, i. e. the presence of a common mechanism able to stabilize or de-stabilize specific transcripts, as for the members of the PUF proteins family [2]. Moreover, we envisage the possibility that our methodology could be used on different data and organisms and thus providing a computational support to the understanding of transcriptional and post-transcriptional networks, given the recent growing interest in the post-transcriptional regulation layer [1] of gene expression (miRNA) and its role in many diseases, such as cancer. Finally, the characterization of the replication complex in terms of lead-lag relationships among gene expression time profiles of its sub-complexes suggests the possibility that our analysis could be effectively used as a tool for predicting the formation of transient multiple protein complexes.
  The mRNA relative abundance time course data obtained from cell populations experiments for gene A and B is denoted by mA and mB, respectively. The simultaneous R2, is the usual squared Pearson correlation coefficient which measures the fraction of the total variance explained by a linear fit between the two variables mA and mB, that iswhere η accounts for intrinsic and extrinsic noise. The rationale behind the lead-lag R2 is the following. We considered two genes, A and B, subject to the same regulatory signal (promoter activity) – possibly of different strength – due to the presence at their promoters of the same TF complex in its active state. Moreoever, we assumed that the change in mRNA levels due to the degradation rate could be reasonably well captured by a first order rate kinetics [53], and consequently the dynamic equation that includes both synthesis and degradation is the following (1) where the two variables mA and mB measure gene expression on a linear scale (fold induction), PX is the promoter activity time profile of the TF complex relative to gene X, αX is its maximal strength, kX is the degradation rate (kX = log (2) /t1/2) and ηX accounts for intrinsic and extrinsic noise. In order to remove size effects, the common signal between the promoter activities of the two genes is indicated as p (t) and is such thatso that we get (2) From the second equation of (2) we haveand substituting it into the first equation of (2) we obtainthat can be rewritten asBy evaluating the time integral of both sides we finally get: (3) whereand coefficient c5 accounts for the integration constant. The lead-lag R2 is the fraction of the total variance explained by model (3). Note that the lead-lag R2 depends on the time order of the data, whereas the simultaneous R2 remains the same after a time shuffling of the data. Moreover, it is worth emphasizing that model (3) may well describe other biologically relevant mechanisms, such as time-shifted profiles as shown in the supporting information file Text S1. In this case, obviously, the coefficients ci, which depend on the underlying model, will change accordingly. Any pair of time profiles, satisfying model (3) will be said to have a lead-lag relationship and a good fit to (3) can be obtained also in situations different from those assumed to derive it. This property is very useful since it provides flexibility in modeling different biological phenomena resulting from the presence of a common regulatory signal. The reason for the term “lead-lag” is due to the fact that two signals satisfying model (3) also define the transfer function of a “lead-lag compensator” widely used in control systems engineering. Assuming, for the sake of simplicity, the signals devoid of linear trends and noise (c4 = c5 = δ = 0), model (3) in the Laplace domain is as follows: which can be rewritten as: so that the resulting transfer function between mA (s) and mB (s) is that of a lead-lag compensator: Let the available experimental time series of two genes A and B be composed of N>5 samples taken at times t1, …, tN. Model (3) can be rewritten, using matrix notation, as followswhere Δ collects all of the noise terms andso that the least square estimation of the parameter vector isAccordingly, the goodness of fit to model (3) is measured bywhere the norm used is the usual Euclidean norm. It is important to note that the lead-lag R2 can be computed directly from gene expression data and values near unity indicates that the model well fits the available time series.
summary: Microarrays provide snapshots of the transcriptional state of the cell at some point in time. Multiple snapshots can be taken sequentially in time, thus providing insight into the dynamics of change. Since genome-wide expression data report on the abundance of mRNA, not on the underlying activity of genes, we developed a novel method to relate the expression pattern of genes, detected in a time-series experiment, using a similarity measure that incorporates mRNA decay and called lead-lag R2. We used the lead-lag R2 similarity measure to predict the presence of common transcription factors between gene pairs using an integrated dataset consisting of 13 yeast cell-cycles. The method was benchmarked against six well-established similarity measures and obtained the best true positive rate result, around 95%. We believe that the lead-lag analysis can be successfully used also to predict the presence of a common mechanism able to modulate the degradation rate of specific transcripts. Finally, we envisage the possibility to extend our analysis to different experimental conditions and organisms, thus providing a simple off-the-shelf computational tool to support the understanding of the transcriptional and post-transcriptional regulation layer and its role in many diseases, such as cancer.
keywords: cell biology/gene expression
  computational biology/transcriptional regulation
  molecular biology/mrna stability
  molecular biology/bioinformatics
  computational biology/systems biology
title: Embedding mRNA Stability in Correlation Analysis of Time-Series Gene Expression Data