article: Molecular recognition by intrinsically disordered proteins (IDPs) commonly involves specific localized contacts and target-induced disorder to order transitions. However, some IDPs remain disordered in the bound state, a phenomenon coined “fuzziness”, often characterized by IDP polyvalency, sequence-insensitivity and a dynamic ensemble of disordered bound-state conformations. Besides the above general features, specific biophysical models for fuzzy interactions are mostly lacking. The transcriptional activation domain of the Ewing' s Sarcoma oncoprotein family (EAD) is an IDP that exhibits many features of fuzziness, with multiple EAD aromatic side chains driving molecular recognition. Considering the prevalent role of cation-π interactions at various protein-protein interfaces, we hypothesized that EAD-target binding involves polycation- π contacts between a disordered EAD and basic residues on the target. Herein we evaluated the polycation-π hypothesis via functional and theoretical interrogation of EAD variants. The experimental effects of a range of EAD sequence variations, including aromatic number, aromatic density and charge perturbations, all support the cation-π model. Moreover, the activity trends observed are well captured by a coarse-grained EAD chain model and a corresponding analytical model based on interaction between EAD aromatics and surface cations of a generic globular target. EAD-target binding, in the context of pathological Ewing' s Sarcoma oncoproteins, is thus seen to be driven by a balance between EAD conformational entropy and favorable EAD-target cation-π contacts. Such a highly versatile mode of molecular recognition offers a general conceptual framework for promiscuous target recognition by polyvalent IDPs.
  Understanding the sequence-function relationship of a protein and how it might malfunction is central to biomedical research. While many proteins function in their folded states, recently it became clear that intrinsically disordered proteins (IDPs) also play key functional roles [1], [2] in transcription, translation and cell cycle regulation that, when altered, frequently lead to cancer [3]. Indeed, ∼70% of proteins implicated in cancer are predicted to have significant disordered regions [3], [4]. Molecular recognition by IDPs typically involves target-induced folding. Intriguingly, however, certain IDPs engage in protein-protein interaction without coupled folding and binding [5] such that the IDP remains disordered even when bound to a globular target. This phenomenon has been termed “fuzziness” [6] and is characterised by IDP polyvalency, sequence-insensitivity and lack of strict geometric complementarity for binding [6]. Important examples of fuzziness include transcription factors [7], linker histones [8], prion-like proteins [9] and Sic1-Cdc4 in yeast [10]. To gain insight into “fuzzy” interactions, we have studied the Ewing' s Sarcoma (EWS) -activation domain (EAD) in the TET family of RNA-binding proteins [11] and Ewing' s family of oncoproteins (EFPs). EAD is a ∼280 residue polyvalent IDP comprised mainly of a degenerate repeat motif SYGQQS. Studies of EAD have mostly focused on its role in naturally occurring EFPs in which it is fused to various transcription factor partners. EFPs are potent EAD-dependent transcriptional activators, resulting in distinct phenotypes of the associated Ewing' s family of tumors [12], [13] which are largely dictated by the DNA-binding domain of the EWS fusion partner. Progress in understanding EAD has been hindered by its IDP properties [14] and a general lack of biophysical/biochemical insights [15]. Another barrier is the paucity of information regarding cognate EAD-interacting proteins. Because native EWS interacts with a highly complex array of proteins at a network hub [16], [17] or potentially as a scaffold protein [18], it is probable that EAD has numerous partners. Functional studies of EFPs have provided a foundation for understanding sequence-function relationship of EAD. Most notably, the transcriptional and oncogenic activity of EAD is conferred by multiple tyrosine (Y) residues due to their aromaticity but not hydrophobicity [14]. EAD function is also markedly sequence-insensitive [14], although a permissive overall composition is apparently required. This type of interaction shares features with other systems that exploit polyvalent IDP phosphorylation, as in autoinhibition of CFTR [19], auto-regulation of Ets-1 transcription factor [20], [21] and interaction of Cdk inhibitor Sic1 with its E3 ubiquitin ligase Cdc4 [10]. Sic1 has nine low-affinity Cdc4-binding sites and a threshold number of phosphorylated sites induces highly cooperative “polyelectrostatic” binding of Sic1 to a single positively charged pocket in Cdc4 [10], [22]–[24]. Similarly, EAD activity requires cooperative action of multiple aromatic moieties in a disordered structure [14], [25], though it does not require phosphorylation. Thus molecular recognition by EAD was coined “polyaromatic” [26]. However, the physical basis for polyaromatic EAD function has not been elucidated. In light of the versatile roles of cation-π interactions in protein folding and protein-protein interactions [27]–[37], we hypothesized that a major contribution to molecular recognition by EAD (within EFPs) is the attraction between numerous unconstrained aromatic residues (π' s) on the EAD and basic residues (cations) on the target. We tested this idea experimentally and also theoretically in molecular simulations that are based on cation-π contacts between the EAD and a generic folded target. We found broad agreement between EAD functionality and simulated EAD binding. Thus our findings strongly support the polycation-π model and suggest that similar mechanisms might also be exploited by other IDPs.
  In view of the abundance of aromatic residues in EAD (38 Ys in the native EAD) and the significant strength of cation-π interactions [38], we posit cation-π as a highly plausible and probably most significant type of EAD-target contacts. This leaves open the possibility of additional contributions but these are likely to be secondary [14]. To probe the nature of EAD-target interactions we designed EAD mutants with different numbers, placements and types of aromatic residues in conjunction with EAD charge variations (Fig. S1). In vivo transcriptional activity of EAD mutants was compared, relatively, with computational predictions of binding probability assuming the polycation-π hypothesis. In the absence of specific knowledge about EAD targets, a generic globular target with appropriate surface charge was used for simulations to provide a minimalist physical model for the proposed interactions. The intact EAD spans ∼280 highly repetitive residues but such a long sequence is not particularly amenable to mutagenesis and is also quite impractical for computational studies. This hurdle can be overcome by exploiting small EAD regions (∼40 residues) that faithfully mimic the salient features of the intact EAD and whose transcriptional activity (transactivation) can be readily tested using a multisite reporter [14], [25]. To establish a framework for experiments, we began by functionally testing a 66-residue polypeptide (10Yn) with ten Y residues, which is closely related to part of the native EAD, and the corresponding series of mutants (4Yn–9Yn) varying only in Y number () but retaining the same Y density (Fig. 1A). Transactivation was quantified by a well-established transient assay in Jeg3 cells with EAD sequences fused to the DNA-binding domain of zta protein and a zta reporter plasmid (pZ7luc) [39] (Methods). As for other EAD sequences studied before [25], transactivation rises in a nonlinear manner with (Fig. 1C, red circles), demonstrating that multiple Ys act together in a cooperative manner. To assess the polycation-π idea, we constructed a coarse-grained chain simulation model that embodies the hypothesis. The EAD is represented by a flexible chain and a generic globular target protein is modeled as a sphere with surface charge distribution (Fig. S2A, B) resembling that of the RNA polymerase II subunits Rpb4/Rpb7 (PDB id: 2C35; Fig. S2C), which was reported to bind to EAD [40], [41]. Binding is driven by EAD-target cation-π contacts (Fig. S2D), the interaction energies (Fig. 1B and Fig. S2E) of which are consistent with published estimates of cation-π potentials of mean force in aqueous environments, with attractive well depths ≈−3. 0 to −5. 5 kcal/mol [32], [33], [38]. In accordance with PDB data [30], [32], contacts between one cation and multiple aromatics or between one aromatic and multiple cations are allowed; but the orientation dependence [42] and nonadditivity [43] of cation-π interactions are neglected. Because EAD-target cation-π interactions are suggested to be highly dynamic with bound EAD remaining disordered, we included an average solvation effect [38] rather than considering the discrete water configurations that impact on cation-π interactions [44]. Our model also incorporates electrostatic and intra-EAD hydrophobic effects (Fig. S2F) by using potential functions similar to those developed for coarse-grained protein folding simulations [45], [46]; but EAD-target hydrophobic interactions were not considered because of insufficient knowledge about the real target. Binding probability () was determined using Monte Carlo sampling (see Methods and Supporting Text S1 for details). Fig. 1C shows that the simulated Pbs rationalize the functional data regarding the effect of Y (aromatic) number. A similar agreement with model simulation was also observed for the activities of a set of previously studied EAD sequences (Fig. S3). Noting that the EAD-zta proteins used for determining activity are dimers whereas EAD monomers were used in our simulations, we also verified that the EAD monomer and dimer values have a similar Y-number dependence (Fig. S4), indicating that EAD monomer simulations are adequate for capturing behavioral trends of the corresponding EAD dimers. We emphasize that the experimental-theoretical comparisons in Fig. 1 and subsequent figures are between relative experimental activities and relative. The model binding free energy, where is Boltzmann constant and T is absolute temperature (Fig. 1D), is dependent upon the effective EAD concentration (see below). However, the latter is unknown experimentally and our simple model does not account for every physical interaction between the real EAD and its target. Thus, it is not meaningful to compare absolute against absolute experimental activity. Nonetheless, by assuming that putative unknown factors affect different EAD sequences similarly (Text S1), one may compare the differences in simulated for various EAD sequences with the corresponding differences in EAD activity. Doing so yielded a good agreement between experiment and theory for the 4Yn–10Yn sequences (Fig. 1D), lending support to the polycation-π hypothesis. To better understand how EAD binding might be affected by various assumptions about the target and multisite IDP binding in general, we developed a simple analytical model to complement the chain simulations. Briefly, our analytical model considers an IDP chain of n contour length units with equally spaced aromatic residues that are k units apart, and a target with cations. When the IDP is distant from the partner, it can adopt conformations with any residue fixed in space; that residue in turn can access a volume V (i. e. , the IDP concentration is). Binding is favored by an energy (<0) for each IDP-target cation-π contact. A bound IDP has ≥1 such contact, with possible pairings for the first contact. Because the volume accessible to the first contacting residue is reduced from V to a small “capture” volume and the number of IDP conformations is reduced from to a smaller because of IDP-target excluded volume, it follows that the change in free energy upon forming the first contact is. For >1, further cation-π contacts can lead to IDP loops of various lengths (where = 1,2, …; Fig. S5A) spanning a variety of distances between different cations on the target (Fig. S5B). If is the number of IDP conformations of length n with such a loop and is the number of instances of, the free energy of binding is approximately given by: (1) where we have used the number of conformations with a mid-chain attachment for, neglecting the small variation in that depends on the attaching point (Text S1); thus is the conformational reduction factor for forming an IDP loop. in represents all possible sets of ≥2 aromatic residues that can contact the target (resulting in 1 to loops). The term vanishes when = 1. is over the different loops for a given set of contacting residues. We assumed that the loops are independent and neglected the excluded volume repulsion among them. Exact enumeration of self-avoiding lattice flights [47] (Figs. S5C–E, S6, Supporting Tables S1, S2, S3) and extrapolations of such data (Fig. S7) were applied to estimate the conformational entropy terms involving in Eq. (1). Further details of the model are provided in Text S1. Salient features of the analytical model are shown in Fig. 2. An essentially linear dependence of on is seen (Fig. 2A) as for the simulation results (Fig. 1D). As expected, a stronger (more negative) leads to tighter binding (more negative). The binding equilibrium is governed by a balance between favorable cation-π contacts on one hand and translational and conformational entropy on the other (Fig. 2A, inset). Binding increases with aromatic density, IDP concentration C (Fig. 2B, D; C∼), and target cation density (Fig. 2C). Fig. 2A shows that the trend for ≈−3. 5kBT in our analytical model matches approximately the behavior of in the chain simulation in Fig. 1D. This value of ≈−2. 1 kcal/mol (for T = 300 K used in this study) is comparable but weaker than the average pairwise cation-Y energy ≈−3. 3 kcal/mol we determined from our simulation using a cation-Y potential energy well depth of ≈3. 6 kcal/mol (Fig. 1B). This discrepancy is not unexpected because excluded volume effects among the loops are neglected in Eq. (1), resulting in an overestimation of binding probability. Nonetheless, the overall trend exhibited by the chain simulation model is well reflected by the analytical model. In addition to accounting for Y-number dependence (Fig. 1), the cation-π hypothesis also rationalizes EAD activity of mutants with Y substituted by phenylalanine (F) or tryptophan (W). Statistical analysis of PDB structures [32] and quantum calculations [48] have indicated that the cation-Y and cation-F strengths are similar, with F slightly weaker [48], but cation-W is significantly stronger (Text S1). Consistent with this trend, Fig. 3A shows that the experimental activity of 5Fn is slightly lower than that of 5Yn [25], but the activity of 5Wn is ∼8 fold that of 5Yn. Simulated for these sequences using the corresponding cation-π energies in Fig. 1B mirror these experimental observation, lending further credence to the polycation-π hypothesis. We next investigated the effect of altering EAD charge. First, we changed anion composition by introducing aspartic acid (D) residues (Fig. 3B). Adding 3 Ds to 10Yn (10Y3D) or adding 5 Ds to the minimally active 5Y (5Y5D) barely changes activity. The fact that anion additions do not enhance EAD activity rules out favorable contacts between EAD anions and target cations as a major driving force for binding. Second, we changed cation composition by introducing arginine (R) residues (Fig. 3C). Inasmuch as the 66-residue EAD peptides are flexible as posited by our chain simulation model, the inserted Rs would allow intra-EAD cation-π contacts and thus reduce activity by competition. Fig. 3C shows that an EAD with 10 Ys and 5 Rs (10Y5R) is indeed much less active than one with only 8 Ys and zero Rs (8Yn) and is comparable with a protein containing only 5 Ys. Similarly, 8Y2RΔD (containing 8 Ys and 2 Rs) is comparable with 6YΔD (6 Ys and zero Rs) and both EADs are approximately 3-fold less active than 8YΔD (8Ys and zero Rs). Apparently, the addition of R residues within the EAD functionally counteracts Ys in an essentially one-to-one manner. This finding is highly suggestive of Y-R contacts between EAD and real target proteins and thereby strongly supports the cation-π hypothesis. The relative simulated values broadly capture the activity trends for charge variations (Fig. 3B, C). Quantitative agreement between simulation and experiment is seen for 10Y3D, 5Yn, 6YΔD, and 8Y2RΔD. Simulation also accounts for the near-irrelevance of anion number for 5Y and 5Y5D activities (Fig. 3B). Simulations did however slightly overestimate the decrease in activity caused either by reduction of Y number from 10 to 5 (Fig. 3B, compare 10Yn with 5Y or 5Y5D) or by introduction of cations into 10Yn (Fig. 3C, compare 8Yn with 10Y5R). The average EAD-target electrostatic energy is essentially neutral or very slightly repulsive in our model (+0. 2 kcal/mol). Because of the dominance of cation-π over electrostatic interactions (Fig. S2E, F), of 10Yn and 10Y3D are very similar; but there is some EAD-target electrostatic repulsion due to the anions on 10Y3D, resulting in a slightly weaker average EAD-target cation-π energy for 10Y3D compared with 10Yn (−22. 2 vs −23. 4 kcal/mol). Intra-EAD cation-π interactions in the unbound state are strong in R-containing mutants, amounting on average to −31. 9 kcal/mol for 10Y3R and −67. 6 kcal/mol for 10Y5R and are slightly weaker in the bound state (−24. 9 and −63. 6 kcal/mol respectively). The favorable EAD-target cation-π energy acquired upon binding is −18. 5 kcal/mol for 10Y3R and −7. 0 kcal/mol for 10Y5R on average, indicating that the weaker binding of 10Y5R is caused by increased competition from intra-EAD cation-π interactions due to the larger number of Rs present. As shown in Fig. 2B, the polycation-π hypothesis envisions that EAD activity depends on both aromatic number and density. We tested this prediction using EAD sequences with constant Y number (= 7) but different Y densities (1/k values). The data in Fig. 4A show both experimental activity and simulated binding diminish with decreasing Y density 1/k. This trend is consistent with the analytical model results for these sequences (Fig. 4C, diamonds), although the analytical model predicts a less pronounced decrease. Is EAD activity affected by altering the sequence positions (distribution) of the Ys while maintaining overall density and total Y number? In Fig. 4B, sequence 5YP has a total of 10 Ys arranged as 5 pairs separated by ∼12 residues and has transcriptional activity similar to 10Yn (88%) and in excess of 40-fold more active than 5Y. Simulations (Fig. 4B) and the analytical model (Fig. 4C, squares and circle) generally reflect the activity trend but overestimate for 5YP compared with 10Yn. This mismatch probably results from the simplifying model assumption that each individual cation-π contact for two adjacent Ys interacting simultaneously with the same cation is equivalent to an isolated cation-π contact, whereas in reality adjacent Ys would each have somewhat weaker interaction due to steric hindrance by each other and the orientation dependence of cation-π interactions ([42] and Text S1). Taken together, these results indicate that Y density influences EAD activity but that Y distribution is not crucial. Physically, weaker binding at lower Y densities arises from at least two conformational entropy effects that result from longer loops between cation-π contacts: formation of an individual longer loop is entropically more costly than a shorter loop [47], and excluded volume interference between longer loops is also more severe. Both effects disadvantage longer loops and disfavor binding of EAD sequences with lower Y densities.
  The significance of protein disorder in the bound state or “fuzziness” has only recently emerged [6]. Theoretical modeling of IDPs [22], [49]–[67], especially for fuzzy complexes [22], is also in its infancy but provides powerful tools for understanding dynamic conformer ensembles. Our integrated functional and computational approach has culminated in a distinctive model for fuzzy interactions (Fig. 5) that may contain the core features of a more general mode of protein-protein interaction. The model involves a simple biophysical contact (cation-π), strong cooperativity stemming from both IDP and target polyvalency, and a highly flexible and dynamic IDP conformer population in the bound state. Stable binding requires a sufficient number of cation-π contacts but allows kinetic exchanges between myriad bound states. Notably the molecular recognition events studied here are particular to the diseased state of EFP-induced malignancies and are therefore of immediate biomedical interest. Our hypothesis is intuitive given that cation-π interactions have wide and versatile biological roles, the interaction is strong [38] and EAD is highly polyvalent. The native intact EAD is also virtually devoid of cationic residues and thus especially amenable to trans cation-π interactions with target proteins. Here, our interrogation of the polycation-π model covered a wide range of EAD sequence properties (variations of Y number, cation-π strength, charge, Y density, and Y distribution) as well as simulation parameters (physically relevant variations of the cation-π, hydrophobic, and electrostatic interaction strengths; see Text S1). In all these tests, the polycation-π hypothesis provides a consistent biophysical account of the experiments. Other types of interactions are much less likely to contribute dominantly to EAD-target binding and our experiments address some of these. Of particular interest is the stoichiometric intramolecular blocking of Ys by Rs within EAD (Fig. 3C). This observation argues against alternative EAD-target aromatic interactions such as π-π stacking which are, in any event, probably of insufficient strength [68] in the absence of proximate metal ions [69] to account for the slope of Y number dependence of EAD activity (Fig. 1). One may also imagine a scenario in which EAD compaction is induced by Y-dependent hydrophobic interactions such that EAD-target contacts may involve poorly defined non-aromatic entities. But this possibility is strongly contraindicated by the high degree of EAD disorder [14] and also by our finding that EAD compaction by introduction of R residues (Text S1) actually decreases activity. Potential hydrogen bonding effects are not addressed in our model due to insufficient experimental data. Intuitively, hydrogen bonding involving prevalent EAD residues (Gln, Ser, and Thr) may well contribute to molecular recognition by EAD, although previous data [14], [25] together with the current study indicate that cation-π interactions are the essential driving force. More refined studies will be required to uncover secondary and more subtle contributions to EAD-target binding, including potential couplings between hydrogen bonding and cation-π interactions [70]. We also stress that our results do not preclude additional effects due to EAD posttranslational modifications, including tyrosine phosphorylation and O-GlcNAcylation [71], that might sometimes be manifest for particular EFPs and/or in specific physiological circumstances. We have assumed a globular target because the biophysical aspects of the proposed model strongly predict that a large number of real globular proteins interact with EAD. Nonetheless, a disordered (IDP) target that enables favorable cation-π contacts with the EAD is also possible (Fig. S8) although so far the fuzzy complexes known to involve two IDPs are homodimers [72], [73]. We cannot infer how many cation-π contacts are required for EAD binding to real targets. It is also likely that particular interactions will deviate in some manner from our generic model. One can envision a variety of target determinants that might have an impact, including, for example, number and/or density of cations, acute geometric constraints imposed by residues flanking target cations, and the contribution of other aromatic side chain interactions such as hydrogen bonding. Polyelectrostatic (Sic1/Cdc4) and polycation-π interactions share some similarities. Each may well reflect a general mode of interaction for polyvalent IDPs. In contrast to Sic1-Cdc4, however, the properties of the EAD studied herein are related to the diseased state [14] and our study points to several significant biophysical differences between EAD and Sic1/Cdc4. First, Sic1/Cdc4 binding involves a single Cdc4 site while EAD binding in our model invokes multiple simultaneous contacts. Second, Sic1/Cdc4 interaction is switch-like, reflecting the biological need for acute response to cell cycle kinase levels, whereas the EAD is constitutively polyvalent [14], [25]. Third, like most other polyvalent IDPs, Sic1 has short sequence-specific or linear motifs [74], [75], a single copy of which can mediate suboptimal or high-affinity Sic1/Cdc4 binding [10]. Such elements are almost certainly absent in EAD [14]. Fourth, the multiple cation-π contacts that underpin EAD binding in our model entail transient restrictions of EAD conformations (though they remain disordered), whereas a Sic1 bound to a single Cdc4 pocket at a given instant is not subject to such conformational restriction [22]–[24]. The molecular recognition events studied here are related to pathological EAD function and, accordingly, are not obviously shaped by evolution [14]. Some aspects of EAD malfunction are an indirect consequence of loss of the EWS RNA-binding domain (RBD) or gain of a foreign DNA-binding domain in EFPs. In relation to our study, it is intriguing that the EWS RBD contains highly disordered regions with reiterated RGG that autorepress EAD [76], quite possibly via intramolecular masking [40]. The polycation-π perspective may offer a rationalization for this behavior. The simulated binding between a disordered EWS peptide containing multiple RGG boxes and the 10Yn EAD indeed reveals a strong interaction (Fig. S8). Intramolecular cation-π interactions between EAD and RGG have high potential to impact native EWS function by competing out aberrant interactions between EAD and the putative globular proteins relevant to EAD malfunction in oncogenesis. In this regard, knowledge of EAD-target interface might provide therapeutic avenues [77] for Ewing' s family tumors with poor prognosis. Several small molecule inhibitors of EWS/Fli1 have been identified. Interestingly, they all have aromatic character [78]–[80] or, in one case, a very basic short peptide sequence [81]. Whether any of them target the EAD portion of EWS/Fli is unknown. Due to their likely being effective cation-π competitors, it will be of great biomedical interest to explore this possibility. How may polyaromatic molecular recognition by EAD relate to normal EWS protein function? This is a challenging question given the strong evolutionary conservation of EWS [82] that includes several EAD properties: a positionally conserved Gln two residues C-terminal to Y, Y phosphorylation sites [83], and SH2/SH3 interaction sites. However, none of these features are required, at least in some cases, for oncogenic EAD function [14]. Perhaps the mode of EAD action in EFP oncoproteins reflects a primordial polyaromatic function that was subsequently tailored by evolution to fulfill normal cellular roles. For example, Y phosphorylation can dramatically increase the aromatic-cation interactions required for peptide inhibitors of Src [84], indicating that phosphorylation of only a limited number of Ys in EAD could have profound effects on EAD-target interactions that are important for normal EWS. To conclude, the proposed model for molecular recognition by EAD expands the seemingly endless modalities for IDP function and malfunction. The hitherto unrecognized polycation-π mode of IDP-target binding can be versatile. It offers a highly plausible biophysical basis for EAD and perhaps other scaffold/networking proteins to interact with many distinct target proteins [16]–[18]. The present methodology and results can also be extended to facilitate the exciting search for real EAD targets.
  Plasmids: pZΔE [25] and pZ7Luc [39] are previously described. All other plasmids expressing EAD variants were derived from the mammalian expression vector pSliencer 4. 1-CMV neo (Applied Biosystems). Proteins: pZΔE expresses a protein lacking EAD sequences and containing only the ATF1 region and zta bZIP domain [25]; see Fig. S1. Transactivation assays and Western blotting: Transfections, trans-activation assays and quantitation of transactivation under linear assay conditions were performed as previously described [25]. Activity values were corrected for background activity determined by including the EAD-negative protein ZΔE in transfections. Details for plasmid and EAD construction and the assays are provided in Text S1. The EAD is modeled as a Cα chain. Pairwise interactions between amino acid residues depend on whether they are aromatic, hydrophobic, charged, or polar (see Text S1 and Fig. S2E, F for definition). The generic EAD-binding target is a sphere of radius 16. 0 Å with 32 positively and 32 negatively charges on its surface (Fig. S2A). The total energy of the model system is the sum of the intramolecular energy within the EAD and the intermolecular energy between the EAD and its target. The expressions for these energy functions, other modeling details, and control simulations are provided in Text S1.
summary: Understanding how proteins recognize each other is central to deciphering the inner workings of living things and for biomedical research. It has long been known that the sequence of a protein, which is a string of different amino acids, can dictate how a protein molecule folds into a well-defined shape required for biological tasks. Many folded proteins recognize and bind with each other by a tight geometric fit similar to that between a lock and its key. Recently, however, it has become clear that some proteins function as a flexible string, in constant motion, without forming a stable shape. Understanding how such “disordered” proteins work is challenging. To gain insight, we studied a disordered protein region that causes a large family of human cancers. Employing an innovative combination of experimental and theoretical techniques, we describe a new mode of protein interaction based on multiple simple contacts between one type of amino acid (aromatic) in the disordered protein and another type (positively charged) on the partner protein. Because this mechanism also underlies the ability of the disordered protein to cause cancer, further investigation of this unprecedented mode of protein-protein interaction may open up new avenues for cancer therapy.
title: Polycation-π Interactions Are a Driving Force for Molecular Recognition by an Intrinsically Disordered Oncoprotein Family