article: Quorum sensing (QS) enables bacterial multicellularity and selective advantage for communicating populations. While genetic “switching” phenomena are a common feature, their mechanistic underpinnings have remained elusive. The interplay between circuit components and their regulation are intertwined and embedded. Observable phenotypes are complex and context dependent. We employed a combination of experimental work and mathematical models to decipher network connectivity and signal transduction in the autoinducer-2 (AI-2) quorum sensing system of E. coli. Negative and positive feedback mechanisms were examined by separating the network architecture into sub-networks. A new unreported negative feedback interaction was hypothesized and tested via a simple mathematical model. Also, the importance of the LsrR regulator and its determinant role in the E. coli QS “switch”, normally masked by interfering regulatory loops, were revealed. Our simple model allowed mechanistic understanding of the interplay among regulatory sub-structures and their contributions to the overall native functioning network. This “bottom up” approach in understanding gene regulation will serve to unravel complex QS network architectures and lead to the directed coordination of emergent behaviors.
  Biological phenomena are frequently controlled by an entangled web of protein and gene networks that constitute regulatory pathways. A large number of such pathways take advantage of environmental cues and signaling molecules to regulate cellular activities. However it is not always clear how biological systems are able to support accurate signal propagation over a sufficiently large dynamic range within the cell. One important factor in determining the fidelity of a signal, in any biological system, is the connectivity of the network, e. g. , the interactions among the constituent genes, proteins and metabolites. Recent studies indicate that certain patterns of local connectivity such as negative and positive feedback motifs are more frequently found in natural systems [1], [2]. Despite their individual abilities to influence the flow of information, negative and positive feedback loops are often coupled together in natural systems. One example is the Xenopus embryonic cell cycle where a negative feedback loop and a pair of positive feedback loops control the operation of a robust tunable cell cycle oscillator [3]. Several synthetic oscillators have also been built to demonstrate the robust behavior that results from the interaction of various feedback motifs [4]–[6]. In this paper, we examined network connectivity in a natural network which also employs a combination of negative and positive regulation and, at the same time, provides cell-cell communication among bacterial cells. Here, an auto-regulatory network is coupled with a double negative motif to provide a population based response known as bacterial quorum sensing (QS). We examined the network connectivity and followed the propagation of the native signal molecule, autoinducer-2 (AI-2), in this system. Different regulatory motifs of the network were first studied in isolation by constructing mutant strains and were then combined to represent the system as a complete network. Using a combination of experimental work and mathematical modeling, we isolated the mechanisms of AI-2 transport into the cells and investigated how the interplay of the different feedback mechanisms orchestrates the overall cell-cell communication and population based regulation. Our experimental work predicted the existence of a new regulatory element that was not previously suggested. We tested our hypothesis by building a model based on the experimental results that also incorporated the new regulatory element. Our predication was captured well by the model and we were able to simulate both the intact network and all the sub-systems using our new proposed network architecture. Importantly, we revealed the mechanistic basis for the LsrR-mediated genetic “switch” of E. coli QS circuitry. In the native system, the switch is buried but still effective. Our modeling results demonstrated the basis for and biological importance of a well-regulated network wherein a balance between the strengths of different feedback motifs is required for the proper functioning of the overall system.
  In general, QS can be described as a density-dependent cell-cell communication process among bacteria that is mediated by the transmission and propagation of chemical signals known as “autoinducers” [7]–[9]. Autoinducers are synthesized within the cell cytoplasm, secreted to the outside and accumulate in the cells’ immediate surroundings [10]. At a point associated with a “quorum” of cells, where the cell density and hence the concentration of the exported autoinducer reaches a threshold, the signaling molecules are transported back into the cells or are bound to cognate cell surface receptors, where they initiate coordinated changes in gene expression [11]. Several classes of signaling molecules and QS mechanisms have been identified [12]. The focus of this work was to investigate the transduction of autoinducer-2 (AI-2) which is the dominate form of cell-cell communication in E. coli and S. Typhimurium. AI-2 is also suggested to be a “universal” signal molecule due to the presence of its terminal synthase in over 80 genera [8]. Figure 1 summarizes the AI-2 processing mechanism in E. coli (for more detailed description, see [13]). During cell growth, AI-2 is synthesized through a multi-step enzymatic pathway and transported out of the cell membrane of individual bacterial cells [14], [15], Figure 1A. The increasing bacterial population results in the accumulation of AI-2 within the extracellular milieu. Once the concentration of AI-2 reaches a critical “threshold” it is transported back into the cell [16], [17], triggering a coordinated genetic response, Figure 1B. Like many biological phenomena, the complexity of the interactions within the QS process, makes the detailed study of this system challenging. We used a “bottom-up” approach to reduce the complexity of the network by separating it into smaller sub-systems (modules) and examining each sub-system in isolation. A more comprehensive picture of the overall network behavior and their interactions could then be elucidated by combining the findings of these modules. We began by separating the AI-2 synthesis/export module from the AI-2 uptake/regulation module shown in Figure 1. Since AI-2 synthesis has been modeled in previous work [13], we focused on understanding the mechanisms of AI-2 transport back into the cell and its transduction/actuation potential. Experimentally, the two modules could be separated by removing one of the AI-2 synthase genes, luxS, from the genome and halting the in vivo AI-2 synthesis. AI-2 was then synthesized in vitro and its concentration estimated by measuring free thiol groups [18]. The synthesized AI-2 was then added to the system at different concentrations and dose response curves were determined. There are two significant advantages to using this in vitro approach. First, the separation of these two modules simplifies our analysis by eliminating cross-interaction between in vivo synthesized AI-2 and AI-2 that is transported in from the outside. The synthesized AI-2 was treated as an external input added into the system in defined concentrations and hence the exact amount of AI-2 present in the initial system was known. Second, this is a more quantitative approach to study QS mechanisms when compared to studies that rely on measurements of in vivo synthesized AI-2. In vivo AI-2 studies typically performed using an indirect cell-based assay employing Vibrio harveyi bioluminescence [19] which has a high level of day-to-day variability and its irregularities have been noted [20], [21]. As a result, we suggest that our approach is a more quantitative method for the purposes of characterizing network interactions within the QS system. The regulatory elements pertinent to the AI-2 uptake mechanism have been identified in a number of previous studies [15]–[17], [22] and are summarized in Figure 2A. External AI-2 is transported back into the cell through at least one known transporter, Lsr, encoded by the lsr-operon. The lsr-operon in E. coli encodes 6 genes that are responsible for the AI-2 uptake and modification mechanisms. The first four genes (lsrA, lsrC, lsrD, and lsrB) encode the import apparatus and the last two genes (lsF, lsrG) are putative AI-2 processing genes [17], [22] that eliminate the activated form of AI-2 (phospho-AI-2 or AI-2-P) from the cytoplasm in a similar manner to that of S. Typhimurium with a highly homologous system [15]. Experimental evidence also indicates that in the absence of the Lsr transporter, AI-2 is can be transported into the cell via an as yet uncharacterized pathway [15]. To account for this unidentified transport mechanism, we included an alternative pathway (denoted “Alter”) in our scheme (Figure 2A). Upon entering the cell, AI-2 is phosphorylated by a cytoplasmic kinase, LsrK, and the phosphorylated AI-2 interacts with a transcriptional regulator, LsrR [23]. LsrR inhibits transcription of the lsr-operon by binding to the lsr-operon promoter site. LsrR also acts an auto-regulator by binding the lsrR promoter site and inhibiting its own transcription [17]. Phospho-AI-2 reportedly binds the LsrR protein and prevents inhibition of the lsr-operon, hence alleviating repression and increasing Lsr transporter production. An increase in the levels of the Lsr transporter expedites AI-2 uptake and creates a positive feedback loop where higher concentrations of AI-2 within the cell result in an increase in AI-2 uptake. Figure 2B depicts the dose response curves for 7 different experiments in which various concentrations of AI-2 were added to growing cultures of E. coli cells that lacked the luxS gene. Expression levels from lsr-operon promoter, which indicate LsrR activity, were measured using a β-galactosidase (β-gal) assay, as described in the Materials and Methods. As expected, higher AI-2 levels resulted in higher expression rates from the operon. As cells reached the stationary phase, the expression levels of all cultures decreased as the in vitro AI-2 levels and the cells’ metabolic activity also decreased. To better understand the role of each of the regulatory elements presented in Figure 2A and explain the experimental results of Figure 2B, our next step was to systematically remove each of the three key regulatory elements within the AI-2 uptake module and reduced the network into three sub-modules. Each sub-system was constructed by removing either lsrR, lsrK or lsr-operon genes from the genome of strains that already lacked the luxS gene. In each of these mutant strains we observed the expression level of the lsr-operon promoter as the output. In accordance with previous experimental work [17]) and our experimental observations (data not shown), the deletion of any of these genes has no affect on the cell growth. Once these sub-systems were analyzed in isolation, the individual sub-system responses were compared with the overall network response that was seen in Figure 2B. Our analysis, which is summarized in the following sections, predicted the existence of an additional lsr-regulatory mechanism and a new AI-2/protein interaction that had not been hypothesized (or identified) until now. The construction of the first sub-system involved the removal of the entire lsr-operon from a bacterial strain that already lacked the luxS gene, as shown in Figure 3A (experimental details of gene deletion are provided in Materials and Methods). In this sub-network, AI-2 is not transported into the cell via the Lsr-operon transporter but it can still enter the cell using the alternative pathway [19]. The AI-2 which enters the cell via the Alter pathway, is phosphorylated by LsrK (AI-2-P), binds the LsrR protein and prevents the repression of the lsr-operon promoter by LsrR. We experimentally monitored the flow of AI-2 by measuring the lsr-operon activity over time. Figure 3A shows the temporal responses of lsr-operon expression levels from 7 different cultures where various concentrations of in vitro AI-2 were added to the growing E. coli cultures. As the concentration of AI-2 increases, more AI-2-P is available to bind LsrR resulting in higher transcription from the lsr-operon promoter. This accounts for the higher expression levels observed for cultures that are induced with higher AI-2 concentrations. By 5 hr, most of AI-2 is taken up by the cell and the lsr-operon expression reaches its highest level. By 6 hr, external AI-2 is depleted and expression levels decline as cells enter the stationary phase. We observed one surprising result within this sub-system - that the levels of expression were higher without the lsr-operon (Figure 3A) compared to what was observed for the intact network (Figure 2B), at the same concentrations of AI-2. The highest level of sub-network 1 was ∼200 Miller units compared to the overall system behavior (∼70 Miller units). The second sub-system was built by removing the lsrR gene from a bacterial strain that already lacked the luxS gene (shown in Figure 3B). Without the presence of the repressor protein, LsrR, the lsr-operon is not repressed and AI-2 can enter the cell via both the lsr-operon and the alternative pathway. According to this model depiction, the expression level of the lsr-operon is independent of the AI-2 levels and we expected to see high levels of expression from the lsr-operon irrespective of the concentration of AI-2. Figure 3B represents the time response of three cultures grown with different levels of AI-2. Contrary to our prediction, the expression levels were not as high as, nor higher than, those seen in Figure 3A. Further, the system appeared to have a slight dependency on AI-2 concentration as higher levels of expression were found for higher AI-2 concentrations. The last sub-system was built by removing the lsrK gene from the isogenic parent strain. Without the LsrK protein and the corresponding AI-2-P in the system, LsrR is free to fully repress the lsr-operon. Therefore, we expected that the lsr-operon expression levels should be low regardless of input AI-2 concentrations. Figure 3C represents the experimental data for 2 samples at both low and high concentrations of AI-2. In agreement with our expectations, the expression levels were both low. The result shown in Figure 3A and 3B indicated that the lsr-operon mutant (Figure 3A) had higher expression levels than the parent strain (Figure 2B) and that lsrR mutant response (Figure 3B) had a slight dependency on AI-2 concentration (Figure 3B). These observations suggested that the perceived network architecture as presented in Figure 2A might not be an accurate depiction of the overall system behavior. Other regulatory elements and possible feedback mechanisms, not identified in previous studies, may be involved in the AI-2 uptake/transduction process. In order to account for such regulations, we constructed a mathematical model of our network and its sub-systems. We started by modeling the AI-2 uptake by the cell and its downstream regulation that were depicted in Figure 2A. Table 1 and 2 summarize the details of this first model. The synthesis of LsrR and Lsr-operon were each modeled by a single equation (Eq. 1 & 2, Table 1) where protein synthesis, decay, cooperative binding and repression were all encompassed in a single equation. AI-2 delivery to the cell was modeled by an active transport of AI-2 by the Lsr-operon and also an alternative pathway that was represented by a simple flux (Eq. 3, & 4, Table 1). Our experiments have indicated that the presence of LsrK is essential to the operation of this network and that in the absence of this kinase the system is shut down. Moreover a kinetic analysis of LsrK activity in a recent paper [24] has shown that the phosphoralytion of AI-2 is rapid and is completed within a few minutes. As a result, to simplify the network description we made the assumption that all the imported AI-2 was phosphorylated (denoted as Ap in the model). AI-2 interaction and binding to LsrR was modeled as a formation of a complex and its eventual decay (Eq. 5, Table 1). Initial concentration of all variables, except AI-2, was set to zero (Table 2). AI-2 was modeled as an input to the system and its concentration varied over a range of 1 to 40 uM to match the in vitro AI-2 concentration used in our experiments. A deterministic solution to the system of ordinary differential equations presented in Table 1 was evaluated using the freely available software COPASI [25]. In the deterministic framework, COPASI calculates time course by using a LSODA integrator [26] that will numerically evaluate a solution to the system. Since few empirical data on the kinetic parameters of this system was available, a parameter estimation routine was used to fit and match the model to the experimental data shown in Figure 2B. A global parameter estimation routine based on a least-squares method and a direct search algorithm [25], [27] was used to minimize the distance between experimental data and this first model. The parameterization routine (the range of parameters are listed in Table S3) was performed on all the kinetic parameters that are listed in Table 2 with the exception of protein decay which was set to cell division time. This assumption is valid as the proteins in this network are generally stable and have half-lives that are much larger than the cell division time [17]. The cell division time was set to 30 minutes based on previous experiments performed in our laboratory and the understanding that mutations do not affect cell growth [17], [22]. Table 2 lists the kinetic parameters that provided the best correlation to the experimental result of Figure 2B. Figure S1 represents the dynamic response of this corresponding first model for 10 AI-2 concentrations as described in Text S1. As it is evidence from these graphs this model is a good representation of the experimental data. We then used the same model parameters to simulate the response of the sub-systems that are presented in Figure 3. The result of these simulations (time responses are shown in Figure S2) was not a good representation of the experimental data. In the model, in the absence of Lsr-operon, the AI-2 uptake is significantly impaired and LsrR is able to repress the operon expression level to very low levels. Since our experimental results of Figure 3A were unexpected, it was not a surprise that the model also did not reproduce the experimental data and that we were unable to simultaneously reproduce the experimental results observed in Figure 2B and Figure 3. A global parameter estimation routine revealed that, using the same model description, several parameters such as k1, k2 and nR needed to be significantly changed in order to reproduce Figure 3A or 3B alone. In order to rectify these discrepancies, several different network topologies were considered as possible alternative configurations for this system. For example the Lsr-operon transport and the alternative pathway might work as mutually exclusive switches where in the presence of Lsr-operon the alternative pathway is shut down. There is also the possibility that LsrR has other regulatory roles in the network that are not well understood. One interaction that was hypothesized and showed promise in our preliminary evaluations was the existence of a second regulatory mechanism (in addition to LsrR) that controls the transcription of the lsr-operon. In theory, an lsr-regulator protein may act as a negative regulatory mechanism to repress the activity of the lsr-operon promoter site. The original model (denoted first model) was modified to include this interaction. The modification involved introducing a protein named “REG” as a second repressor in the system and adding a repression term within the Lsr-operon synthesis equation. A new parameter estimation routine was performed to correlate the model with both the experimental result shown in Figure 2B and Figure 3 simultaneously. The new model allowed us to replicate the experimental results in Figure 2B and 3A quite well (time courses shown in Figure S3 and explained in Text S1) but it did not support the weak dependency of lsr-operon expression on AI-2 that was demonstrated in Figure 3B. We looked for other possible scenarios that could account for this apparent weak dependency on AI-2 in the absence of LsrR. One possible explanation, as shown in Figure 4A, is that the lsr-regulator [28], similar to LsrR, could bind AI-2-P and reduce the repression of the lsr-operon in the presence of high concentrations of AI-2. This could result in higher expression levels from the promoter in the absence of lsr-operon (seen in Figure 3A) and an increase in expression levels with the addition of AI-2 (seen in Figure 3B). This is conceptually feasible, as described later, as REG protein processing can both eliminate AI-2-P as a positive regulator over long periods of time and it can also sequester AI-2-P while it is bound. Using the described network architecture (Figure 4A) we were able to produce simulation results that matched all our experimental results closely (see Figure S4 for time response simulations). Table 3 describes the new set of equations in this modified model and Table 4 lists all the kinetic parameters that were used. In order to show the comparison between the model and experiments more clearly we plotted the promoter activity as a function of the AI-2 concentrations at a single time point (time of maxima in simulations shown in Figure S4 and experiments shown in Figure 2 and 3) as shown in Figure 4B and 4C respectively. This representation captures the effect of AI-2 on individual sub-networks and at the same time allows us to compare the different sub-networks within the network. Our simulation results matched our experiments closely and indicated the possible existence of the interaction between the lsr-operon, its own promoter and the AI-2-P. After a close examination and deciphering of the AI-2 uptake mechanism it was interesting to go back and compare our results with the responses of wild-type E. coli. We compared the lsr-operon expression level of luxS knock-out cells (−/+ in vitro AI-2) with the wild-type expression levels. Expression levels for the wild-type and the high in vitro AI-2 supplemented cases were similar (Figure 5A, left y-axis). For reference, we depicted the measured AI-2 activity of V. harveyi within the extracellular milieu of the cultivated wild-type cells on the same graph (Figure 5A, right y-axis). As expected, AI-2 is accumulated and upon reaching a threshold, it is transported back into the cell. The highest level of AI-2 activity for the wild-type cells, measured through the AI-2 assay (see Materials and Methods), was around 200 units of luminescence. We then measured the activity levels of different in vitro AI-2 concentrations to find the level of in vitro AI-2 that yielded expression levels as that in the in vivo synthesized AI-2 case (Figure 5B). To our surprise, we noticed that an in vitro concentration of only 4 µM gave similar AI-2 activity as the wild-type. For the higher in vitro AI-2 concentrations we observed greater luminescence (around 1,000 units of luminescence). According to our results in Figure 2B, 4 µM of in vitro AI-2 can only marginally de-repress the lsr-operon and the expression levels of the promoter in this case are much lower than what is seen in the wild-type strain. This is a very important observation as it points out the possibility that AI-2 might not be completely exported out of the cell in the wild-type cells and that the internally made AI-2 might also contribute to the regulatory mechanisms of AI-2 uptake [17], [29]. Furthermore, in addition to being a signaling molecule, AI-2 might have other regulatory and metabolic roles within the cell. Further investigation of the internal AI-2 is required to shed light on some of these observations.
  The AI-2 uptake mechanism, which is one of the two main modules in the AI-2-mediated QS system in E. coli, was taken apart in this work and the sub-systems were analyzed in isolation. We separated our original network architecture into simpler modules, examined each module separately and this led to the speculation of new regulatory interactions within the network. Several different interactions including the existence of other active transports, a mutually exclusive switch like behavior of the Lsr-operon transport and the alternative pathway and enzymatic activity of other proteins that might degrade AI-2 were all considered and examined in a preliminary test. One scenario that showed promise was the case in which an lsr-regulator protein would interact with the lsr-operon promoter site and act as a negative regulator (based on the results obtained in Figure 2B and 3A). In addition the response of the lsrR knock-out strains (Figure 3B) suggested a possible interaction of the lsr-regulator protein with AI-2-P. These new interactions were incorporated into the construction of a comprehensive system of ordinary differential equations, details of which are summarized in Tables 3–4. Four types of regulations played an important role in determining the outcome of this model i) the LsrR repression of the lsr-operon (represented by k1), ii) the auto-regulatory effect of LsrR on its own promoter sites (represented by k2), iii) interaction of lsr-regulator (REG protein) with the lsr-operon promoter site (represented by k4), and iv) AI-2-P binding to LsrR and REG (represented by k3 and k5). The regulation of LsrR and REG (negative feedback motifs) were coupled with the AI-2-P binding of the LsrR and REG (where the binding acts as de-repressing mechanism and hence has an overall positive feedback effect) to give this network a combination of both negative and positive feedback regulation. Our modeling results indicated that only with in a specific range of kinetic rates we were able to produce the desired response that correlated well with all the experimental results. The best correlation between the model and the experiments occurred when the relative affinity of the LsrR for the lsrR promoter was of the same order as the LsrR affinity for the lsr-operon promoter. This indicates that LsrR has similar binding affinity for its own promoter and the lsr-operon promoter site and that a competitive dynamics exists within this network architecture to bind LsrR repressor protein. The degree of regulation of the lsr-operon by REG protein was less than the LsrR auto-regulation strength and the binding affinity of AI-2 for the lsr-regulator was also lower than its affinity for the LsrR protein. This indicates that LsrR plays a more significant role in regulating the dynamics of the QS network suggesting that this protein might be involved in other metabolic pathways within the cell as speculated by others [29]. Our further analysis of the system revealed that the in vitro AI-2 levels required to produce similar effects on the system are very high compare to what is expected based on the secretion of in vivo AI-2. This observation suggests that AI-2 might have other regulatory roles within the cells that are still unknown. The two most important revelations in this work are: the apparent negative feedback regulation on the lsr-regulator that is as strong as or stronger than that of the LsrR protein, and the uncovering of the strength of the Lsr “switch”. Negative regulation is a recurring motif in biological networks and previous works have shown that this motif is able to reduce transcriptional noise in single genes and cascades [30]–[32] and increase the fidelity of signal transmission in biological networks [33], [34]. In our case, the mechanistic basis for the lsr-regulator’s effect on repression is only partially explained. The regulator’s processing of AI-2-P would understandably sequester and reduce AI-2-P, enabling higher transcription of the lsr-operon. However, our conjecture that an lsr-regulator is repressing the promoter site via direct binding or other mechanism is supported by several currently disjoint observations. First, we found LsrR binding to both the lsrR and lsr-operon promoters were predicted to be similar in strength. This suggests coordination between LsrR proteins, which would be feasible if LsrR operates as a dimer [35] and these sites are brought into proximity. Additionally, since the data from the lsr-operon mutant are significantly higher than the lsrR mutant, the net effect should be the recruitment of other factors, not currently considered. We hypothesize two such scenarios: first, we have shown here the effects of in vivo AI-2 are significantly different than imported and phosphorylated AI-2. We have also demonstrated that unphosphorylated AI-2 plays a role in modulating LsrR regulated gene expression [29]. In the lsr-operon mutant, AI-2 is transported into the cell via alternative pathway, presumably phosphorylated by LsrK, but not degraded by LsrFG. This will alter the relative ratio of AI-2 and AI-2-P which might influence lsr expression in an as yet undetermined manner. Second, in ours [17] and Bassler’s previous report [16] there is significant interplay between CRP and lsr expression, and binding sites in the intergenic region were revealed. Perhaps the lsr-operon and the AI-2-P state alter the effects of CRP in this regulatory switch. We note that the presence of glucose completely shuts off lsr expression and swamps the QS regulation mediated by LsrR [17]. The second most important revelation was that the LsrR “switch”, in fact, is significantly stronger when turned on in sub-networks than in the wild-type cells. In effect, we have found the levels of AI-2 needed to toggle this switch and hypothesize that it might be of use in guiding phenotype. Switch-like behavior is a common phenotype of network topologies that have some degree of positive regulation or feedback [36], [37] and in our case the binding of the repressor protein to AI-2-P effectively plays this role and is the further evidence of the existence of a switch. Our work is the first study of QS wherein a combination of double knock-outs and in vitro synthesized AI-2 have been used to quantify gene regulation in the E. coli AI-2 system. We showed that using a bottom-up approach and isolating the important regulatory elements is an effective way to analyze a natural biological network especially when positive and negative regulations exist within the network architecture. We also showed that the study of such isolated modules allows one to construct a hypothetical model of the system and use simulations to predict the existence of new regulatory mechanisms. Further analysis of this network architecture will shed light on other regulatory pathways within the metabolic networks of bacterial cells and may be used to guide phenotypes in new ways as the quorum sensing switches become incorporated into various biotechnological applications.
  The bacterial strains used in this study are listed in Table S1. Luria-Bertani broth [38] contained 5 g of yeast extract (Sigma) liter−1,10 g of Bacto tryptone (Difco) liter−1, and 10 g of NaCl liter−1. Media were supplemented with antibiotics at the following concentrations: Ampicillin, 20 µg ml−1; Kanamycin, 10 µg ml−1 and Chloramphenicol 10 µg ml−1. The one-step replacement method described by Datsenko and Wanner [39] was used to construct a luxS deletion in E. coli strains LW8, LW9 and LW11. The phage λRed recombination system was used to replace the luxS gene with a luxS: : Crm PCR fragment. pKD3 plasmid was used as PCR template with primers luxSHP1 and luxSHP1 (Table S2). The PCR products were then treated with DpnI and introduced by electroporation into E. coli LW8, LW9 or LW11 strains containing pKD46 plasmid. The strains were then grown in 37°C for an hour. Recombinants were selected on LB plates supplemented with Kanamycin and Chloramphenicol. The deletion of the genes was verified by PCR tests. Cultures of E. coli were grown overnight in LB, diluted 100-fold into fresh LB, grown to the OD600 below 0. 05. The cultures were incubated at 37°C with shaking at 250 rpm and grown for 1 hour. AI-2 was then added to the system and samples were places back in the incubator. Samples were removed at hour intervals for determination of the OD600 and β-galactosidase activity using the Miller method [40]. The Specific activity of β-galactosidase was expressed in Miller units [40]. Cell-free culture fluids were prepared by centrifugation of the E. coli samples culture at 10,000 rpm for 5 min in a microcentrifuge. Cleared supernatants were filtered (0. 2 µm size HT Tuffryn filters; Pall Corp. , Ann Arbor, Mich.) and stored at −20°C. These cell-free culture media were tested for the presence of AI-2 by inducing luminescence in Vibrio harveyi reporter strain BB170. The assays were performed as outlined by [41].
summary: Quorum sensing is a mechanism by which bacterial cells communicate within a population. One particular form of communication in E. coli is through a universal signaling molecule known as autoinducer 2. Although the importance of this form of cell-cell interaction has been recognized in the formation of biofilms and virulent infections, the mechanisms by which this form of communication is regulated is still not well understood. In this paper, we presented a method of unraveling these mechanisms by using a combination of experimental work and mathematical models. We took apart the network architecture and isolated the different components. The examination of these isolated sub-networks provided us with a better understanding of the underlying mechanisms that control and regulate bacterial quorum sensing. We were also able to predict new network interactions with the help of our mathematical models. This bottom up approach, combined with our modeling efforts, proved effective in unraveling the mechanisms of quorum sensing in E. coli.
keywords: biotechnology
  bioengineering
  systems biology
  biological systems engineering
  biology
  computational biology
  genetics and genomics
  engineering
title: LsrR Quorum Sensing “Switch” Is Revealed by a Bottom-Up Approach