article: In eukaryotes, RNA polymerase II (PolII) dependent gene expression requires accessory factors termed transcriptional coregulators. One coregulator that universally contributes to PolII-dependent transcription is the Mediator, a multisubunit complex that is targeted by many transcriptional regulatory factors. For example, the Caenorhabditis elegans Mediator subunit MDT-15 confers the regulatory actions of the sterol response element binding protein SBP-1 and the nuclear hormone receptor NHR-49 on fatty acid metabolism. Here, we demonstrate that MDT-15 displays a broader spectrum of activities, and that it integrates metabolic responses to materials ingested by C. elegans. Depletion of MDT-15 protein or mutation of the mdt-15 gene abrogated induction of specific detoxification genes in response to certain xenobiotics or heavy metals, rendering these animals hypersensitive to toxin exposure. Intriguingly, MDT-15 appeared to selectively affect stress responses related to ingestion, as MDT-15 functional defects did not abrogate other stress responses, e. g. , thermotolerance. Together with our previous finding that MDT-15: NHR-49 regulatory complexes coordinate a sector of the fasting response, we propose a model whereby MDT-15 integrates several transcriptional regulatory pathways to monitor both the availability and quality of ingested materials, including nutrients and xenobiotic compounds.
  Eukaryotic gene transcription requires the concerted interplay of many factors. DNA-binding factors nucleate specific regulatory complexes on individual genes, culminating in assembly of functional RNA polymerase II (PolII). These complexes also contain transcriptional cofactors that serve various functions, such as chromatin remodeling and chromatin modification. Within this complex machinery, the sequence specific regulatory factors are generally thought to be the primary determinants that specify transcriptional output in response to a certain signal. The Mediator is a conserved multi-protein coregulatory complex that, at the minimum, serves a critical linking function between regulatory factors and the transcription initiation machinery [1]–[3]. Some Mediator subunits, such as yeast Med17 are required for essentially all PolII-driven transcription [4]. Similarly, in the nematode Caenorhabditis elegans the subunit MDT-14/RGR-1 is broadly required for early embryonic transcription [5]. However, in both organisms, some Mediator subunits are required only for expression of a restricted subset of all PolII-transcribed genes [4]–[7]. Indeed, many Mediator components influence specific physiological and/or developmental processes. For instance, mammalian MED1/TRAP220 is utilized by nuclear hormone receptors (NHRs) to implement programs such as adipogenesis (through peroxisome proliferators activated receptor γ (PPARγ) [8]) and systemic detoxification (through the pregnane-X-receptor (PXR) and the constitutive androstane receptor (CAR) [9], [10]). Likewise, the C. elegans Mediator subunits MDT-12/DPY-22, MDT-13/LET-19, and MDT-1. 1/SOP-3 participate in vulva or male tail development [7], [11]–[13]. These studies raise the question of how individual components within the same regulatory complex can exert such separable effects. Also, it is unclear whether the specific functions of the coregulators are broader or narrower than those of the sequence specific regulatory factors with which they interact. Moreover, although certain Mediator subunits differentially affect related functions, the relationship between Mediator subunit utilization and a transcriptional mechanism or a physiological process is not known. Deciphering the mechanistic contributions of individual Mediator components to transcription is relevant in view of Mediator' s conservation and its capacity to interact with numerous regulatory factors, thus influencing many biological processes [14]. In a previous study we found that the C. elegans Mediator subunit MDT-15 integrates expression of certain metabolic genes in NHR-49-dependent and -independent ways [15]. Others found that MDT-15 conveys regulation of fatty acid (FA) desaturases by the basic helix-loop-helix zipper protein SBP-1, the C. elegans ortholog of the mammalian sterol regulatory element binding proteins (SREBPs) that regulate FA and cholesterol metabolism [16]. Therefore, NHR-49 and SBP-1 appear to collaborate with MDT-15/MED15 to affect overlapping yet distinct sectors of metabolic genes. Hence, MDT-15 exhibits a broader spectrum of physiologic regulation than either individual regulatory factor, and could be viewed as an important node in a regulatory network that maintains metabolic homeostasis. Thus, analysis of individual Mediator components might reveal both upstream regulatory inputs and downstream regulatory mechanisms within a critical gene network. To connect the function of MDT-15 in transcription to its precise physiologic role, we sought to more broadly define MDT-15' s sphere of influence. As an initial step, we set out to globally discover new MDT-15-dependent genes in an unbiased fashion, and thus to identify previously unrecognized biological processes that lie downstream of MDT-15.
  We previously showed that the C. elegans Mediator subunit MDT-15 impacts expression of select genes involved in fatty acid (FA) metabolism [15]. To identify MDT-15' s physiological targets in an unbiased manner, we used expression microarrays to compare the transcriptional profiles in wild-type (N2) worms that were fed for 40 hr (i. e. allowed to synchronously develop to larval stage L4; see Materials and Methods) with bacteria harboring vectors for either control or mdt-15 RNA-interference (RNAi; reviewed in [17]). To identify statistically significant changes in gene expression following MDT-15 depletion we analyzed the raw data using “linear models for microarray data” (limma [18], [19]; see Materials and Methods). In total we found 187 genes that were downregulated, and 120 genes that were upregulated (P-value cutoff 0. 05; Table S1). An alternative approach, “significance analysis of microarrays” [20], revealed similar numbers and similar sets of affected genes (data not shown). These numbers support the hypothesis that MDT-15 is essential for expression of only a subset of genes in C. elegans [15]. Given that MDT-15 and its mammalian orthologs have thus far been characterized as coactivators [15], [16], [21], we focused this study on genes with reduced mRNA levels in mdt-15 (RNAi) animals. The genes identified as downregulated in mdt-15 (RNAi) animals included 12 previously identified MDT-15 target genes involved in lipid metabolism [15], and mdt-15 itself, thus validating our experimental approach (Figure S1, and Table S1). In order to verify previously unrecognized candidate MDT-15-targets, we performed quantitative real-time PCR (qPCR) analysis on total RNA from worms grown on either control RNAi or on mdt-15 RNAi bacteria (see Materials and Methods). In total we tested 85 of the 187 downregulated candidate MDT-15 targets by this method, primarily focusing on genes with orthologs in mammals, and genes predicted to participate in metabolism and detoxification (see below). We found that 63 of 85 genes (74%) were downregulated more than two-fold (Figure S1 and Table S2; note that, in this validation assay, the two-fold cut-off yields false negatives, as our limma analysis assessed significant change without a specific two-fold minimal change threshold). Thus, our microarray-based strategy was an effective tool to identify novel MDT-15 targets. To corroborate the data obtained with RNAi depletion of endogenous MDT-15 we obtained a strain carrying a mutation in the mdt-15 gene, mdt-15 (tm2182) (strain XA7702; see Materials and Methods and Figure S2A). This strain recapitulates the phenotypes evoked by mdt-15 RNAi, including shortened life span (Figure S2B), reduced fecundity (Figure S2C), clear appearance, and altered fat storage (determined by Nile Red staining [22]; data not shown). We determined by qPCR the relative mRNA levels of 97 MDT-15 targets (85 new candidate genes from the microarrays and the 12 previously known MDT-15 targets [15]) in wild-type N2 and mutant mdt-15 (tm2182) worms harvested at the L4 stage. We found that 50 of 97 genes (52%) exhibited more than two-fold deregulation in mdt-15 (tm2182) mutants (Figure S1 and Table S3); 47 of these 50 genes were also deregulated in mdt-15 (RNAi) worms. In contrast, 28 genes deregulated more than two-fold in mdt-15 (RNAi) worms were not affected to this extent in mdt-15 (tm2182) mutants, although most genes were still downregulated to some extent (and only two were upregulated in mdt-15 (tm2182) worms). Thus, mdt-15 (tm2182) mutants recapitulate many but not all of the gene expression defects exhibited by mdt-15 (RNAi) worms. Overall, the gene expression changes in mdt-15 (tm2182) worms appear less severe than those presented by mdt-15 (RNAi) worms, suggesting that mdt-15 (tm2182) may represent a hypomorphic allele. Alternatively, as the mutation is predicted to produce a truncated MDT-15, and as mdt-15 mRNA levels appear normal in mdt-15 (tm2182) worms (Table S3), it is possible that truncated MDT-15 dominantly interferes with Mediator complex function. In any case, the data support our results obtained by RNAi depletion of endogenous MDT-15 and also establish the mdt-15 (tm2182) mutant as a valuable tool to study MDT-15 function. To identify pathways and molecular functions common to the 187 genes observed by microarray analysis to be downregulated after MDT-15 depletion, we employed the DAVID gene ontology (GO) annotation tool [23]. As expected, we found a significant overrepresentation of GO-terms for functions related to lipid metabolism (Table 1). In addition, we used DAVID to query the same gene set for protein domains. We found that domains involved in lipid metabolism were overrepresented (e. g. FA-Δ9-desaturases and FA-oxidases). These results confirm the importance of MDT-15 in regulation of lipid metabolic genes. Unexpectedly, we also discovered other enriched domains subject to MDT-15 dependence, specifically UDP-glucuronosyl/UDP-glucosyltransferases (UGTs; 15 genes of the 187 MDT-15 candidate MDT-15 dependent genes), glutathione S-transferases (GSTs; five genes), short-chain dehydrogenase/reductase SDRs (DHSs; five genes), and FAD-domains (two genes; see Table 2). Although proteins containing any of these domains may metabolize lipids, they are typically associated with systemic metabolism and clearance of endo- and xenobiotic compounds (see below). In addition to these predicted detoxification enzymes, the gene set exhibited overrepresentation of proteins containing the DUF227 domain (six genes). Drosophila melanogaster proteins harboring DUF227 domains are implicated in insecticide resistance [24], and some D. melanogaster DUF227 genes are induced by Phenobarbital [25]. In total, MDT-15 dependent genes are enriched for five protein families associated with detoxification. In addition to the statistically overrepresented protein families, the MDT-15 dependent genes included three cytochrome P450s (CYP450s), three alcohol and aldehyde dehydrogenases, two ABC-transporters, and two acyl-CoA-synthetases (ACSs). These are all members of protein families associated with detoxification. Interestingly, the ACS proteins represent a class of lipid metabolizing enzymes, some members of which can metabolize xenobiotics [26]. Of note, we previously found that two other acs genes, acs-2 and acs-11, respond to short-term fasting in an MDT-15 dependent fashion [15], [27]. Finally, the list of MDT-15-dependent genes included four genes (a metallothionein, mtl-2; a cadmium-responsive gene, cdr-6; a predicted zinc-transporter, T18D3. 3; and a predicted selenium-binding protein, Y37A1B. 5) likely involved in heavy metal detoxification. In summary, 47 of 187 genes (25%) that our microarray analysis identified as significantly downregulated in mdt-15 (RNAi) worms may be involved in metabolism or elimination of toxic substances (Table S1). This number is similar to the number of MDT-15 dependent genes related to energy metabolism (38 genes, i. e. 20%), suggesting that both processes compose important sectors within MDT-15' s sphere of influence (Table S1). DAVID analysis of the 120 genes upregulated in mdt-15 (RNAi) worms revealed fewer enriched functions and domains (Table S4). Among these, upregulation of genes with functions/domains in energy metabolism and in protein folding/degradation may occur as compensation for (metabolic) challenges imposed by MDT-15 depletion. In addition, we noted the enrichment of CUB and saposin domains, both of which have been associated with pathogen defense [28], [29]; it may be interesting in future experiments to examine these genes in the context of MDT-15' s biological role. MDT-15 is highly expressed in the intestine [15], [30], which is an organ important for nutrient ingestion and digestion in C. elegans, and is also thought to be the principle organ for detoxification [31]. Thus, one might expect that MDT-15-targets preferentially exhibit intestinal expression. To address this notion we compared the set of genes identified by our microarrays as downregulated in mdt-15 (RNAi) worms to published sets of genes exhibiting tissue specific expression in C. elegans [32], [33]. We found that many of the genes downregulated in mdt-15 (RNAi) worms are intestine-selective (22%); in contrast, no MDT-15 target genes were enriched in muscle or germ line, and only 2% were pharynx-specific (Table S5). Comparison of our gene set to a C. elegans gene expression map integrating 553 individual microarray experiments [34] also revealed enrichment for intestinal genes, and depletion of germline, neuronal, and muscle specific genes (data not shown). Finally, the 120 genes induced in mdt-15 (RNAi) worms also exhibited enrichment of intestine-selective genes (data not shown). This selective overrepresentation among MDT-15 targets of intestinally expressed genes fits well with the roles of MDT-15 in lipid metabolism [15], [16], and detoxification (see below). MDT-15 is required for both basal and fasting-induced transcription of certain energy metabolism genes [15]. We reasoned that, because MDT-15 is essential for basal expression of several predicted detoxification genes (Table 3 and Tables S1, S2, S3), it might similarly be critical for toxin-activated gene expression. Consistent with the notion that C. elegans ugt, gst, dhs, and cyp genes (encoding CYP450s) are important for detoxification, several of these genes are transcriptionally induced when wild type worms are exposed to toxic chemicals (reviewed in [35]). Interestingly, this includes ugt-1 and cyp-35C1 [36] whose basal expression we found to be MDT-15 dependent (Table 3 and Tables S1, S2, S3). To test whether MDT-15 is required to induce these detoxification genes, we fed N2 wild-type worms for 40 hr (i. e. L1–L4) with control or mdt-15 RNAi bacteria seeded on plates that contained toxic compounds (fluoranthene or β-naphthoflavone). We chose fluoranthene because it is a naturally occurring carcinogen, and β-naphthoflavone because it is a known inducer of numerous CYP450s [36], [37]. In these worms we then determined the relative mRNA levels of 80 MDT-15 targets by qPCR. We found that fluoranthene and/or β-naphthoflavone induced the mRNA levels of 21 MDT-15 target genes greater than two-fold in control (RNAi) worms (Table 3). Importantly, six of these genes exhibited reduced induction by toxins in mdt-15 (RNAi) worms; for example, fluoranthene induced mRNA levels of gst-5 ten-fold in control (RNAi) worms, but only two-fold in mdt-15 (RNAi) worms (Table 3). However, not all induced genes (e. g. ugt-25) exhibited MDT-15 dependent activation, suggesting that other regulatory pathways also contribute to gene induction by toxins. Moreover, when mdt-15 (tm2182) mutants were exposed to fluoranthene for 40 hr (i. e. L1–L4), we observed defects in fluoranthene-induced mRNA accumulation for 13 of the 21 genes, corroborating the results obtained with RNAi (Table 4). The selectivity of MDT-15 action on these 21 detoxification genes is summarized in Figure S3, which demonstrates the weak correlation of toxin induction (particularly the response to fluoranthene) between control (RNAi) and mdt-15 (RNAi) worms (panel A) and wild type and mdt-15 (tm2182) worms (panel C). Taken together, these data demonstrate that MDT-15 is required not only for basal, but also for toxin-activated transcription of select detoxification genes. Surprisingly, our microarray analysis revealed only three cyp genes to be MDT-15 dependent (of a total of 80 C. elegans cyp genes encoding CYP450s). This may be attributable, at least in part, to the low expression levels of many cyp genes. We therefore used qPCR to examine directly the expression of 43 C. elegans cyp genes, and to explore a potential role for MDT-15 in their regulation (Table S6). We found that 14 of these 43 genes required MDT-15 for basal expression in N2 L4 worms. Moreover, 15 of the 43 cyp genes were induced greater than two-fold by fluoranthene and/or β-naphthoflavone; this selectivity of cyp gene induction suggests that some cyp genes may respond to distinct toxins, or may function in roles other than detoxification. Importantly, the induction of nine of 15 toxin-responsive cyp genes was strongly reduced in mdt-15 (RNAi) worms (Table S6, Figure S3B). These results show that MDT-15 is critical for both basal and activated transcription of detoxification CYP450s, thus strengthening the notion that it plays a vital role in the toxin response. MDT-15 depletion reduces worm viability and causes larval arrest [15], raising the possibility that the reduced capability of mdt-15 (RNAi) worms to express and appropriately induce detoxification genes is an indirect effect associated with arrested development and premature death. To address this issue, we determined relative mRNA levels of toxin-responsive genes in mdt-6 (RNAi) worms. We focused on MDT-6 because, (i) like MDT-15, it is a Mediator subunit, (ii) it is required for stage- and gene-specific transcription in C. elegans, and (iii) mdt-6 knockdown evokes larval arrest and premature death, phenotypes reminiscent of but distinct from those of MDT-15 depletion [38], [39]. We found that, in N2 L4-stage worms, mdt-6 RNAi affected neither basal, nor fluoranthene- or β-naphthoflavone-induced expression of detoxification genes (but did reduce mRNA levels of fat-6 and fat-7, as described [15]), suggesting that MDT-6 is largely dispensable for toxin-induced gene transcription (Table S7, Figure S3A). We conclude that larval arrest and premature death alone are not sufficient to prevent the expression of detoxification genes in response to toxins. To assess whether the defects in gene induction by toxins reflected abnormal growth of mdt-15 (RNAi) worms, we employed a conditionally sterile strain, CF512 (fer-15 (b26) II; fem-1 (hc17) III) [40]. To assure that the fer-1 and fem-15 mutations do not compromise the detoxification per se, we first compared the toxin response in CF512 worms to that in N2 worms. We found that, at the L4 stage, CF512 worms exhibited a very similar response to fluoranthene and β-naphthoflavone as did N2 worms (Table S8). Next, we quantified the mRNA abundance of toxin-responsive MDT-15 targets in CF512 worms exposed to mdt-15 RNAi only after completion of embryonic and larval development (adult only RNAi). Under these conditions, 12 of the 20 tested genes exhibited MDT-15 dependence in the absence of fluoranthene. Furthermore, 13 genes were fluoranthene-responsive in these worms, and for four of these genes the induction was at least partially MDT-15 dependent, resembling our results obtained in N2 L4 worms (Table S9). Thus, we conclude that the defective detoxification gene expression in mdt-15 (RNAi) and mdt-15 (tm2182) worms reflects a direct action of MDT-15, rather than a secondary consequence of reduced viability or arrested development. Given that MDT-15 binds to, and collaborates with NHR-49 and SBP-1 to express certain metabolic genes, it seemed conceivable that these two regulatory factors might also be responsible for expression of MDT-15 dependent detoxification genes. To test this hypothesis, we knocked down endogenous NHR-49 or SBP-1 in wild-type worms. As previously demonstrated, nhr-49 (RNAi) and sbp-1 (RNAi) worms exhibited reduced mRNA levels of fat-5, -6, and -7 genes (Table S10). In contrast, these worms were unaffected in their expression of the 20 tested detoxification genes, in unchallenged conditions as well as in the presence of fluoranthene or β-naphthoflavone (Table S10). Indeed, and unlike the pattern exhibited by mdt-15 (RNAi) worms, the overall induction of detoxification genes by toxins strongly correlated with the induction in control (RNAi) worms (Figure S3A). This is noteworthy because sbp-1 RNAi evokes larval arrest and sterility, further strengthening the notion that developmental arrest per se does not impair detoxification. Moreover, worms carrying a mutation in the gene encoding a C. elegans NHR implicated in detoxification (nhr-8; strain AE501 [nhr-8 (ok186) ]; [41]) also failed to deregulate MDT-15 dependent detoxification genes in basal and xenobiotic-challenged conditions (Table S8, Figure S3C). We conclude that NHR-49, SBP-1, and NHR-8 are largely dispensable for MDT-15 dependent expression of detoxification genes, and that MDT-15 likely uses distinct regulatory factors to control expression of detoxification genes. As MDT-15 is essential to activate certain detoxification genes, we hypothesized that mdt-15 depletion or mutation would render worms hypersensitive to toxins. To test this, we grew conditionally sterile CF512 worms on control and mdt-15 RNAi bacteria (RNAi from the L1 stage on) in the presence of various concentrations of toxins, and monitored their development and morphology until day four of adulthood. After this prolonged exposure to mdt-15 RNAi, worms were slightly thinner than control (RNAi) worms, consistent with previous results [15]. We found that fluoranthene synergized with mdt-15 RNAi, but not control RNAi, to evoke an arrest as small, scrawny adults (Figure 1A, B). In contrast, this was not the case for β-naphthoflavone (data not shown). Moreover, mdt-15 (tm2182) mutants exhibited a similar adult arrest phenotype when grown on high concentrations of fluoranthene (Figure S2), whereas wild-type N2 worms showed only a mild developmental delay at the same concentrations of fluoranthene. We conclude that compromised detoxification capability as a result of reduced MDT-15 function causes growth defects in worms challenged with select toxic compounds. Based on their molecular identity or previous studies, the MDT-15 targets cdr-6, mtl-2, T18D3. 3, and Y37A1B. 5 are predicted to contribute to heavy metal detoxification [42]–[45]. Interestingly, transcription of mtl-2 is induced by cadmium (Cd2+) and zinc (Zn2+), but not by copper (Cu2+) [44], [45]. Thus, we tested whether the previously uncharacterized genes Y37A1B. 5 and T18D3. 3 are metal-responsive also. We found that, whereas the Y37A1B. 5 was not strongly induced by any of the tested metals, T18D3. 3 was induced three- to four-fold by Cd2+and Zn2+, but not by Cu2+, thus establishing it as a novel metal-responsive gene (Figure 2A). Given that two of the four metal detoxification genes are metal-responsive, MDT-15 might contribute both to basal and to metal-induced expression of these genes. This hypothesis is supported by the fact that the list of MDT-15-dependent genes (as determined by microarray analysis) overlaps in statistically significant manner with a list of Cd2+-responsive genes ([46]; Table S11). To further characterize MDT-15' s possible role in metal detoxification, we fed wild-type N2 worms for 40 hr (i. e. L1–L4) with control or mdt-15 RNAi bacteria seeded on plates containing Cd2+, Zn2+ or Cu2+. We then probed for induction of the aforementioned genes, and other known heavy metal responsive genes (cdr-1, mtl-1), by qPCR. Consistent with published results, we observed mRNA accumulation of cdr-1, mtl-1, and mtl-2 in the presence of Cd2+, and of mtl-1 and cdr-1 in the presence of Zn2+ (Figure 2A). Importantly, the Cd2+- and Zn2+-dependent induction of mtl-1, mtl-2, cdr-1, and T18D3. 3 was reduced in mdt-15 (RNAi) worms (Figure 2A). Moreover, we found that mdt-15 (tm2182) mutants were also severely defective for both basal and Cd2+-induced mtl-1, mtl-2, cdr-1, and T18D3. 3 expression, and for basal Y37A1B. 5 expression (Figure 2B). Finally, in conditionally sterile worms, adult-only mdt-15 RNAi resulted in reduced basal mtl-1, mtl-2, cdr-1, T18D3. 3 and Y37A1B. 5 mRNA levels and in reduced Cd2+-dependent accumulation of cdr-1 and T18D3. 3 mRNA (Figure 2C). Together, these data demonstrate that MDT-15 plays a critical role in the transcriptional response to heavy metals in C. elegans. In the above experiments, worms were exposed for ∼40 hr to heavy metals, corresponding to chronic conditions. In order to test an acute response to heavy metal, we grew adult worms for 48 hr on control and mdt-15 RNAi, and then challenged them with high concentrations of Cd2+ for five hr. We used a worm strain harboring a transcriptional Pmtl-2: : GFP reporter, CL2122 [47]. This allowed us to rapidly assess mtl-2 promoter activity in vivo. Reminiscent of the results obtained with chronic metal exposure, Cd2+ caused induction of GFP-fluorescence. Importantly, the GFP signal was weaker in worms grown on mdt-15 RNAi, and Cd2+-dependent induction was not observed in these worms (Figure 2D). Thus, MDT-15 is apparently essential for both chronic and acute Cd2+ induced mtl-2 transcription. Moreover, MDT-15 was critical to induce mtl-2 promoter activity specifically in intestinal cells, further supporting the hypothesis that MDT-15 is particularly critical for intestinal gene expression. We have previously reported that a specific nutritional condition, short-term fasting, relies on MDT-15 to induce select genes [15]. This raises the possibility that MDT-15 represents a general stress coregulator. However, our microarrays revealed that mdt-15 (RNAi) worms upregulate known and putative heat-shock proteins (hsp-17 and hsp-16. 2 are α-crystallins, T05E11. 3 is related to HSP90). Thus, in contrast to the aforementioned ingestion-related stresses, MDT-15 might play a negative regulatory role in heat-induced transcription. To test this possibility we grew wild-type N2 worms for 40 hr (i. e. L1–L4) on control and mdt-15 RNAi bacteria at ambient temperature (20°C), subsequently applied heat-shock (i. e. five min, 15 min, and two hr at 35°C), and then quantified the mRNA levels of several hsp genes. At 20°C mdt-15 (RNAi) worms exhibited increased mRNA levels of T05E11. 3, hsp-16. 2, and hsp-17 compared to control (RNAi) worms; we detected similar increases for the mRNAs of the HSPs F44E5. 4 (HSP70), hsp-16. 1, and hsp-16. 49 (Figure 3). Heat-shock failed to affect hsp-4, hsp-17, and T05E11. 3 mRNA levels, but induced F44E5. 4, hsp-16. 1, hsp16. 2, and hsp-16. 49 mRNA levels in control (RNAi) worms (Figure 3 and data not shown); in contrast, mRNA accumulation was not blocked in mdt-15 (RNAi) worms. Instead, mdt-15 (RNAi) worms induced these genes somewhat above the levels exhibited by control (RNAi) worms. Moreover, mdt-15 (tm2182) mutants exhibited a qualitatively similar, yet milder gene expression defect (Figure S4). Thus, our data indicate that MDT-15 is dispensable for heat-shock gene activation, and that it instead suppresses expression of select hsp genes. We do not fully understand the upregulation of both basal and induced expression of these genes upon mdt-15 depletion or mutation, but it seems likely that this is an indirect effect, and that MDT-15 does not act equivalently in all stress responses. Indeed, the increased hsp expression may be an indirect consequence of the detrimental effects of mdt-15 RNAi (which evokes growth arrest and death) rather than a direct effect of deregulation of hsp gene modulators. Taken together, these results demonstrate that MDT-15 is not generally required for stress-activated gene expression, but rather may constitute an element in a regulatory system that specifically monitors availability and integrity of ingested material (see Discussion). As mdt-15 depletion and mutation cause increased expression of heat-shock protein genes, we hypothesized that they might render animals resistant to high temperatures. To test this hypothesis we assessed the survival rate in control (RNAi) and mdt-15 (RNAi) worm populations at 35°C. We found that both populations exhibited similar thermotolerance phenotypes. This was the case in N2 worms exposed to mdt-15 RNAi bacteria from L1–L4 stage (Figure 1C), and also in two-day old conditionally sterile worms exposed to mdt-15 RNAi from the L1 stage on (data not shown). Therefore, the altered heat-shock protein gene expression following mdt-15 depletion is not sufficient to increase resistance to elevated temperatures. This may be due to other gene expression defects in mdt-15 (RNAi) worms. Alternatively, abnormally high heat-shock protein expression in mdt-15 (RNAi) worms may not confer a marginal benefit over heat-shock protein in control (RNAi) animals.
  Like most soil-dwelling animals C. elegans experiences a diversity of conditions, such as insufficient nutrient supply, potentially detrimental substances (endo- and xenobiotics), and harmful microorganisms. Toxic hydrophobic compounds and heavy metals represent a complex challenge as they can produce their adverse effects both acutely, and during chronic accumulation over prolonged exposure periods. Therefore, efficient detoxification mechanisms have evolved to ensure metabolism and elimination of harmful molecules. Here, we provide several lines of evidence that the conserved Mediator subunit MDT-15 is a key contributor towards regulation of systemic detoxification. First, even in the absence of toxic challenge, mdt-15 depletion or mutation results in downregulation of many detoxification-related genes. In addition, MDT-15 is required to induce select detoxification genes in response to hydrophobic toxins and heavy metals. Finally, depletion or mutation of MDT-15 renders worms hypersensitive to at least one toxic compound. Of note, MDT-15 affects expression of all three classes of detoxification genes, i. e. activation enzymes such as CYP450s (class I), transferase enzymes such as the UGTs and GSTs (class II), and transporters such as pgp-7 and pmp-5 (class III [48]). This highlights MDT-15' s broad influence on detoxification and suggests that MDT-15 coordinates entire gene expression programs in response to individual toxic challenges. These data likely reflect a direct contribution by MDT-15, and not an indirect feature of sick/arrested mdt-15 (RNAi) worms, because (i) larvally arrested mdt-6 (RNAi) and sbp-1 (RNAi) worms did not deregulate detoxification genes, (ii) the heat-shock response was unaffected, suggesting that stress-driven transcription can function, and (iii) numerous fluoranthene and cadmium-induced genes were also deregulated in worms exposed to mdt-15 RNAi only after the completion of larval development. Taken together, our data strongly suggest that MDT-15 critically contributes to at least two detoxification pathways in C. elegans. MDT-15 integrates expression of metabolic genes by interacting with at least two metabolic regulatory factors, NHR-49 and SBP-1 [15], [16]. Our findings suggest that MDT-15 may similarly integrate the transcriptional response to environmental stressors such as xenobiotics and heavy metals by employing a different set of regulatory factors. Indeed, MDT-15, and not any single regulatory factor, may constitute the key determinant of the detoxification response in C. elegans. Analogous to metabolic regulation, MDT-15 may collaborate with NHR family regulatory factors to induce specific detoxification programs. Strikingly, the C. elegans genome is lavishly equipped with candidate NHRs [49]. Moreover, NHRs figure prominently in detoxification in metazoans [25], [41], [50]–[52]. In mammals, the NHRs PXR and CAR are particularly critical: by binding structurally diverse compounds [53], they induce expression of appropriate detoxification genes. Notably, both NHRs target the Mediator subunit MED1, which is necessary for induction of detoxification genes in response to drugs and/or toxins [9], [10]. Given that MDT-15 associates with NHRs [15], and modulates transcription in response to xenobiotic compounds (this study), hMED15, the human MDT-15 ortholog, may also contribute to systemic detoxification by CAR and PXR. As the Mediator complex contributes to most PolII transcription, a simple view is that it might function through a single functional domain, or alternatively, that it may somehow operate with little specificity. Instead, the emerging view is that individual Mediator subunits appear to exhibit restricted specificity: For example, in D. melanogaster S2 cells, MED23 is specifically required for heat-induction of hsp26, whereas MED16 is critical to upregulate genes in response to LPS [6]. Similarly, serine 208 phosphorylation of yeast MED2 controls expression of select genes assuring growth under low-iron conditions [54]. More remarkably, our findings demonstrate that a single Mediator subunit appears to “route” transcription, selecting a physiological output appropriate for a given input, in this case energy metabolism in response to nutrient ingestion, or detoxification/transport in response to xenobiotic or heavy metal ingestion. To do so, MDT-15 likely interacts with a spectrum of sequence-specific regulators, as well as particular factors that directly or indirectly control polymerase activity. By incorporating this routing function, MDT-15 takes on a physiological scope that is broader and more sophisticated than any specific regulatory factor. Moreover, for an individual Mediator subunit to communicate with distinct regulatory factors and transcription components under different conditions (while being dispensable in still other circumstances), implies that it may itself receive signaling inputs that drive these distinct behaviors. Therefore, although the composition of Mediator may be similar in all cells and conditions, mere presence of a certain subunit within Mediator may not reflect its specific contributions to regulatory action. Indeed, it seems likely that many Mediator subunits may be subject to various post-translational modifications that could affect their functions. It would be interesting to assess systematically the relative mechanistic contributions of MDT-15 to Mediator' s function under different physiologic conditions. The apparent routing between detoxification and energy metabolism via MDT-15 is notable as others have found that contaminants such as cadmium affect the expression of energy metabolism genes [44], [46], [55]. Although deregulation of metabolic genes may result from perturbed signal transduction [56], [57], regulation of these two processes might conceivably have evolved in a coordinated manner. For example, responses to toxins may impose increased energy expenditure, as detoxification enzymes use ATP, NADH and other energy carriers as co-factors. Similarly, short-term fasting and long-term starvation could cause accumulation of harmful metabolic side-products that necessitate expression of detoxification genes. Accordingly, modulating the activity of a factor such as MDT-15 could ensure appropriate gene expression. Such functional regulation has been described for the mammalian PPARγ-coactivator 1 (PGC-1), which is upregulated by stimuli such as fasting, exercise, and cold exposure. Upon this induction, PGC-1 collaborates with several regulatory factors to confer a switch from reductive to oxidative metabolism [50], [58]. Similarly, detailed characterization of the network that defines MDT-15 action will provide new insights into metabolic homeostasis. It is intriguing that many of the biological processes susceptible to MDT-15 regulation are linked to aging. We previously found that MDT-15 depletion dramatically shortens C. elegans life span, a phenotype partially attributable to reduced FA desaturation [15]. In our present study, we demonstrate a role for MDT-15 in regulation of detoxification, suggesting that compromised toxin elimination might also contribute to the short life span of these animals. Intriguingly, the expression of several genes that we identified as MDT-15 targets is affected by mutations that alter worm life span [59], [60]; moreover, stress tolerance is believed to contribute to life span extension in C. elegans [61], [62]. In summary, we speculate that MDT-15 is an integral part of a regulatory system that coordinates systemic adaptation to ingestion-related stress, thus ensuring maintenance of health and longevity. MDT-15 affects expression of energy metabolism genes that respond to food supply [15], [27], as well as detoxification genes that respond to environmental contaminants (this study); in C. elegans, both activities are directly linked to eating. Although we cannot rule out that some toxins may be absorbed through the worm cuticle, this barrier is generally impervious [63]; moreover, metals are primarily taken up by feeding [64]. Thus, we suggest that MDT-15 is a component of an ingestion-related control system that monitors both the energy availability, as well as the integrity of the ingested material (Figure 4). Such a control system may be particularly beneficial for the soil-dwelling C. elegans, as its feeding during the growth periods may be accompanied by unavoidable co-ingestion of unfavorable substances. As worms may be unable to evade potential harm, or physically separate detrimental compounds, the need for an efficient defense system arises. We speculate that MDT-15 might be an active component of such a regulatory network. Accordingly, in fed worms MDT-15 may cooperate with SBP-1 to drive fat storage and adipogenesis [16], whereas in fasted worms MDT-15 would collaborate with NHR-49 to efficiently cope with short-term fasting [15]. In feeding worms, this same system might invoke “quality control”: hence, MDT-15 and yet unidentified regulatory factors would implement expression of proteins that appropriately metabolize and/or eliminate harmful xenobiotic substances. Moreover, MDT-15 targets such as lectins, GSTs, and lipid metabolism genes have been implicated in the response to pathogen exposure [28], suggesting that MDT-15 may also participate in host defense against microbial infection, another process resident in the worm gut [31]. In summary, we propose that MDT-15 is an essential component of a regulatory network that governs screening and routing of the ingested material to achieve its appropriate utilization, metabolism, and elimination (Figure 4).
  C. elegans strains N2-Bristol (WT), CF512 [fer-15 (b26) II; fem-1 (hc17) III) ] [40]), and CL2122 dvIs15 [mtl-2: : GFP, pPD30. 38 (unc-54 expression vector) ] [47], and AE501 (nhr-8 (ok186) [41]) were maintained as described [65]. The strain XA7702 was generated by out-crossing mdt-15 (tm2182) worms (a gift from Dr. S. Mitani, Tokyo Women' s Medical University) four times with wild-type N2 worms; after isolating homozygotes we verified the presence of the original genomic deletion by PCR with the following primers: 5′-aatgttgctgctcaacgtgc-3′ (forward primer), and 5′-cgatctcttccaattggtcc-3′ (reverse primer). For total mRNA isolation, worm embryos were allowed to hatch on unseeded nematode growth media (NGM) -lite plates overnight at 20°C, and then grown from L1–L4 stages (for 40 hr) on NGM-lite plates containing 25 µg/mL carbenicillin, 4 mM IPTG (Alexis 582-600), and 12. 5 µg/mL tetracycline seeded with the appropriate RNAi bacteria. For validation of candidate MDT-15 targets, we grew worms as follows: control RNAi for 36 hr, control RNAi 24 hr followed by mdt-15 RNAi for 12 hr, control RNAi 12 hr followed by mdt-15 RNAi for 24 hr, or mdt-15 RNAi for 36 hr. Toxins were dissolved in DMSO and added at the following final concentrations (unless indicated otherwise): fluoranthene (Sigma F4418) at 5 µg/ml, and β-naphthoflavone (Sigma N3633) at 6 µg/ml. Heavy metals were dissolved in water and added at the following final concentrations: 3CdSO4*8H2O (Sigma C3266) at 25 µM, ZnCl2 at 100 µM, and CuSO4 at 10 µM. Life span and thermotolerance assays were performed as described [66], [67]. The mdt-15, mdt-6, nhr-49 and sbp-1 RNAi clones have been described [68], [69]. Worms were grown on NGM-lite RNAi plates seeded with E. coli strain HT115 carrying the appropriate RNAi vector. Worms were transferred onto 2% (w/v) agarose pads for microscopic examination. We captured images on a Retiga EXi Fast1394 CCD digital camera (QImaging, Burnaby, BC, Canada) attached to a Zeiss Axioplan 2 compound microscope (Zeiss Corporation, Jena, Germany), and used Openlab 4. 0. 2 software (Improvision, Coventry, U. K.) for image acquisition. Starting with developmentally synchronized L1 stage larvae, N2 worms were grown on control or mdt-15 RNAi for 36 hr to yield synchronized L4 stage populations. Notably, this RNAi protocol did not result in visible death or sickness at the very stage worms were harvested for RNAi isolation. L4 larvae were harvested, washed 5 times in M9, and frozen in liquid nitrogen. RNA was isolated by the Trizol method [69], labeled with Cy3 or Cy5, and hybridized to the WUSTL C. elegans microarrays. We performed five independent biological repeats; one of the arrays was a dye swap. We extracted spot intensities with SpotReader (Niles Scientific), using the default parameters to flag bad spots. To normalize the data, we used RMA background correction and the “NormalizeWithinArrays” function of limma with the print tip loess correction [19]. For a gene to be considered in the final analysis, it had to be unflagged and have a background-subtracted intensity (mean of red and green channels) greater than 64 on at least three of the five arrays. These criteria are non-stringent, particularly the intensity criterion, and may have introduced some false positives, but enabled us to capture expression changes in genes with relatively low expression levels. We used empirical Bayes fitting in limma with multiple hypothesis correction method “BH”, and considered genes with a P-value<0. 05 to be differentially expressed [18]. The microarray data have been deposited in the Gene Expression Omnibus database (http: //www. ncbi. nlm. nih. gov/geo/) under the accession number GSE9720. To determine overlaps between our set of MDT-15 dependent genes and the set of Cd2+-responsive genes [46], we proceeded as follows: As only 10,909 genes pass our stringent spot quality filter in the array analysis, we used this number to estimate the significance of the overlap. This produces a more conservative P-value than using the entire set of genes on the array. The expected fraction of genes that overlap is the fraction of MDT-15 regulated genes multiplied with the fraction of Cd2+-reponsive genes. We used the binomial distribution to calculate statistical significance [70]. Isolation, purification, and reverse transcription of C. elegans RNA have been described ([69] and http: //www. ucsf. edu/krylab/Stefan-WormRNA_isolationqPCR. pdf). QPCR was performed in an ABI7300 PCR machine, and the data analyzed using the Ct method (Applied Biosystems Prism 7700 Users Bulletin No. 2 http: //docs. appliedbiosystems. com/pebiodocs/04303859. pdf). Relative mRNA levels were normalized to act-1 mRNA levels. Primers for qPCR were designed using Primer3 [71]. Primers were tested on dilution series of cDNA, and analyzed for PCR efficiency [72]; primer sequences are available on request.
summary: All organisms adapt their physiology to external input, such as altered food availability or toxic challenges. Many of these responses are driven by changes in gene transcription. In general, sequence specific DNA-binding regulatory factors are considered the specificity determinants of the transcriptional output. Here, we show that, in the roundworm Caenorhabditis elegans, one subunit of a >20 subunit, evolutionarily conserved, non-DNA binding co-factor termed Mediator, specifies a portion of the metabolic responses to a mixture of ingested material. This protein, MDT-15, is required for appropriate expression of genes that protect worms from the effects of toxic compounds and heavy metals. Our previous findings showed that the same protein also cooperates with other regulators to coordinate lipid metabolism. We suggest that MDT-15 may “route” transcriptional responses appropriate to the ingested material. This physiological scope appears broader and more sophisticated than that of any individual regulatory factor, thus coordinating systemic metabolic adaptation with ingestion. Given the evolutionary conservation of MDT-15 and the Mediator, a similar regulatory pathway may ensure health and longevity in mammals.
keywords: molecular biology/transcription initiation and activation
  genetics and genomics/gene expression
  nutrition
  developmental biology/aging
  cell biology/gene expression
title: The Mediator Subunit MDT-15 Confers Metabolic Adaptation to Ingested Material