article: The anaerobic bacterium Fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of Na+. Here, we demonstrate that this ion-motive force is directly coupled to ATP synthesis, via an F1Fo-ATP synthase with a novel Na+ recognition motif, shared by other human pathogens. Molecular modeling and free-energy simulations of the rotary element of the enzyme, the c-ring, indicate Na+ specificity in physiological settings. Consistently, activity measurements showed Na+ stimulation of the enzyme, either membrane-embedded or isolated, and ATP synthesis was sensitive to the Na+ ionophore monensin. Furthermore, Na+ has a protective effect against inhibitors targeting the ion-binding sites, both in the complete ATP synthase and the isolated c-ring. Definitive evidence of Na+ coupling is provided by two identical crystal structures of the c11 ring, solved by X-ray crystallography at 2. 2 and 2. 6 Å resolution, at pH 5. 3 and 8. 7, respectively. Na+ ions occupy all binding sites, each coordinated by four amino acids and a water molecule. Intriguingly, two carboxylates instead of one mediate ion binding. Simulations and experiments demonstrate that this motif implies that a proton is concurrently bound to all sites, although Na+ alone drives the rotary mechanism. The structure thus reveals a new mode of ion coupling in ATP synthases and provides a basis for drug-design efforts against this opportunistic pathogen.
  Synthesis of ATP, the most prominent energy source in biological cells, is largely mediated by the ATP synthase, an enzyme that resides in the membranes of bacteria, mitochondria, and chloroplasts. This enzyme catalyzes the phosphorylation of ADP by a rotary mechanism powered by a transmembrane electrochemical gradient, or ion-motive force, of either H+ or Na+ (proton-motive force [PMF] or sodium-motive force [SMF], respectively). The ATP synthase consists of two sub-complexes: the water-soluble F1 sector [1], [2], which harbors the catalytic centers, and the membrane-embedded Fo complex, which mediates ion translocation across the membrane. These functionally distinct units are mechanically coupled by two additional elements, referred to as central and peripheral stalks [3], [4]. In the Fo sector, eight to 15 copies of subunit c are assembled into a closed ring [5], which rotates around its axis as ions permeate across the enzyme. The c-ring harbors a series of identical ion-binding sites, typically one per c-subunit, which selectively recognize the coupling ion [6]–[8]. Ion binding is facilitated by a conserved carboxylic amino acid, usually glutamate; however, it is the neighboring chemical groups in the protein side-chains and backbone, and sometimes a bound water molecule [9]–[11] that ultimately determine the specificity of the c-ring binding sites [8]. Na+ specific sites typically involve an intricate hydrogen-bonded network of polar groups, while H+-binding sites are simpler, and consist mainly of hydrophobic moieties. Either way, one complete rotation of the c-ring results in the translocation of one ion per binding site and the production of three ATP molecules [12], [13]; the stoichiometry of the c-ring thus defines the ion-to-ATP ratio of the enzyme, i. e. , the minimum ion-motive force required for ATP synthesis [14]. In this study, we characterize the structure, ion specificity, and stoichiometry of the c-ring of the ATP synthase from F. nucleatum, a Gram-negative bacterium implicated in the etiology of periodontal diseases. F. nucleatum grows anaerobically, using amino acids as the preferred carbon source [15]. In particular, glutamate fermentation involves the glutaconyl-CoA decarboxylase, which uses the free energy of decarboxylation to generate a SMF across the cytoplasmic membrane [16], [17]. Comparison of the amino-acid sequence of the F. nucleatum c-subunit with those of other Na+-driven ATP synthases suggests that F. nucleatum utilizes the SMF directly to produce ATP (Figure S1), but this remains to be experimentally demonstrated. Sequence analysis also suggests that ion coordination in the F. nucleatum c-ring could involve not only one but possibly two carboxyl side-chains. This is an unusual and interesting feature, shared by other pathogenic bacteria, whose mechanistic implications are unclear. It is conceivable that the second carboxyl group could alter the assumed ion specificity of the c-ring, the ion-to-ATP ratio, or that it confers a novel coupling or regulatory mechanism to the enzyme [18]. It is also unknown what c-subunit stoichiometry is characteristic of F. nucleatum. Crystal structures have revealed a wide range of possible stoichiometries in H+-coupled c-rings (reviewed in [5]); whether Na+-dependent c-rings from F-type ATP synthases are similarly diverse is unclear, since to date only one such ring has been characterized structurally at high resolution [6]. To clarify these questions, we have isolated the F. nucleatum F1Fo-ATP synthase, and analyzed its ion-coupling mechanism using microbiological, biochemical, structural and computer-simulation methods, both classical and quantum-mechanical.
  To gain insights into the ion specificity of the F. nucleatum ATP synthase, a structural model of its c-ring was created by homology modeling and molecular dynamics simulations (Figure S2). The structure of the c-ring from Ilyobacter tartaricus was used as template [6], [9]. The resulting model thus consists of 11 c-subunits, with an ion-binding site in between each pair of adjacent subunits. Each of these sites includes two glutamate residues, namely Glu32 and Glu65. Glu65, in the C-terminal helix, is the conserved carboxylate found in other c-subunits. Glu32, in the N-terminal helix, is the additional carboxylate found only in selected species. The c-ring was initially modeled in the Na+-bound state. To identify the most plausible conformation of the ion-coordination sphere we constructed several models in alternate protonation states of Glu32 and Glu65, and assessed their likelihood using all-atom simulations of the complete c-ring in a lipid membrane, as well as quantum-mechanical energy calculations for a reduced model of the binding site. In the first of these models (model A), Glu32 is protonated (in cis) and Glu65 is deprotonated (Figure 1A); in this configuration, the binding site is highly similar to that in the crystal structure of the I. tartaricus c-ring [6], [9], except that in that structure Glu32 is replaced by glutamine. Na+ is coordinated directly by oxygen atoms in the side-chains of Glu65, Glu32, and Ser66, as well as by the backbone carbonyl of Val63 and a bound water molecule, which also interacts with Thr67. The proton bound to Glu32 mediates a hydrogen bond to Glu65, which is the acceptor of two more hydrogen bonds, donated by Tyr70 and Ser66. In a second model (model B), Glu32 is deprotonated while Glu65 is protonated (initially in cis). In simulation, this model evolves in time towards a conformation similar to that in model A, with the bound proton (which switches to trans) donating a hydrogen bond to Glu32, and Na+ penta-coordinated (Figure 1B). Lastly, in a third model (model C), both Glu32 and Glu65 were set in the deprotonated state (Figure 1C). This configuration leads to a noticeably different conformation of the binding site, in which only Glu32 and Ser66 coordinate the Na+ ion, while Glu65 is displaced outwards and the bound water molecule reorients. To determine whether model A is more or less probable than model B, we assessed the energetics of proton transfer between Glu32 and Glu65 using Hartree-Fock quantum-mechanical calculations (Figure 2A). These calculations demonstrate that model A is more energetically favorable than model B, by about 10 kcal/mol. That is, if a proton were to be bound to the site, it would be localized on Glu32. To determine whether model A is more or less probable than model C, i. e. , whether a proton indeed resides in the binding site along with Na+, we carried out a calculation of the pKa of Glu32, in the context of the complete c-ring with bound Na+, using Free-Energy Perturbation (FEP) simulations. This calculation shows that the pKa of Glu32 is markedly shifted upwards, relative to its intrinsic value in solution (Figure 2B). Thus, we conclude that in the membrane-embedded, Na+-loaded binding sites of the F. nucleatum c-ring, Glu32 is protonated under physiological conditions. Our predicted structure of the Na+-bound state is therefore that in model A; i. e. , both a sodium ion and a proton are concurrently bound to the same binding site, with the H+ bound to Glu32. The ion selectivity of c-rings in which only one ion can occupy the binding sites is defined by the difference in the binding free-energy of either H+ or Na+ [8], [19], [20]. Because in the F. nucleatum c-ring one additional H+ resides in the binding site in the Na+ state, the analogous free-energy difference is that between a state with two H+ bound and another with one Na+ and one H+. Thus, to examine the question of whether the F. nucleatum ATP synthase is driven by a SMF or a PMF, we re-modeled and simulated the structure of the c-ring with two H+ bound to Glu65 and Glu32, respectively (Figures 3A and S2), and used additional free-energy calculations to assess the likelihood of this doubly protonated state, relative to a state with one Na+ and one H+ bound to the site. Figure 3B shows the computed free energy of selectivity of the F. nucleatum rotor in reference to other c-rings characterized previously [11], [19], [20]. Two alternative models of the H+-bound state were considered (Figures 3A and S2), differing on whether the structural water molecule present in the Na+-bound state is included in the site or not. In both cases, the calculations show that the specificity of the F. nucleatum c-ring is in stark contrast to that of H+-driven ATP synthases. For example, relative to the c-ring of B. pseudofirmus OF4, the selectivity for H+ of the F. nucleatum c-ring is drastically diminished in favor of Na+, by about 26 kcal/mol, i. e. , more than ten orders of magnitude (Figure 3B). Instead, the computations indicate that the ion specificity of the F. nucleatum rotor is comparable to that of the V-type ATPase from Enterococcus hirae, which has been experimentally shown to be very weakly H+ selective [21]. In particular, the F. nucleatum c-ring is more selective for H+ and opposed to Na+ than that of E. hirae, by ∼1–3 kcal/mol, i. e. , up to ∼100-fold. Nevertheless, because in the physiological environment of F. nucleatum (i. e. , the human mouth flora) Na+ is in excess over protons at least by five orders of magnitude, our prediction is therefore that one Na+ and one H+, rather than two H+, will typically occupy the ion-binding sites of the F. nucleatum c-ring. For the same reason the E. hirae ATPase functions as a Na+ pump under physiological conditions. Thus, we conclude that in F. nucleatum the SMF is used to drive ATP synthesis directly. To begin to assess the specificity of F. nucleatum ATP synthase experimentally, we first tested the effect of various protonophores, ionophores, and ATP synthase inhibitors on anaerobic growth (Figure S4). Growth was inhibited by the protonophores carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,4-dinitrophenol (DNP), and 3,3′, 4′, 5-tetrachlorosalicylanilide (TCS), but the sensitivity to these agents varied, with TCS being the most potent. Monensin, a monovalent cation ionophore, was also a potent growth inhibitor of F. nucleatum. Amiloride and its more hydrophobic derivative 5- (N-ethyl-N-isopropyl) amiloride (EIPA), are blockers of Na+ channels and Na+/H+ antiporters, and both compounds inhibited the growth of F. nucleatum. The membrane-permeable ATP synthase inhibitor N, N′-dicyclohexylcarbodiimide (DCCD) also inhibited growth, but tributyltin chloride (TBT-Cl) had no significant effect (Figure S5). Taken together, these data demonstrate that F. nucleatum cells require both a PMF and a SMF to grow, and that classical ATP synthase inhibitors slow down growth. However, these data do not clarify whether the F. nucleatum ATP synthase utilizes the PMF or the SMF to sustain ATP production. To clarify what energy source is utilized by the F. nucleatum ATP synthase, we set out to specifically characterize the enzyme, both in inverted membrane vesicles from F. nucleatum and purified. The ATP hydrolysis activity of the inverted membranes was in the range 0. 09–0. 15 units/mg protein (1 unit = 1 µmol ATP hydrolyzed/min), and exhibited a weak pH dependence, with a pH optimum of 7. 5–8. 5 (Figure S6A). This ATPase activity was strongly inhibited by DCCD (unpublished data), which covalently modifies the carboxyl side-chain of the c-ring ion-binding sites (here Glu65). ATP synthesis in inverted membrane vesicles could be driven by a SMF and under these conditions ATP synthesis was sensitive to both the sodium ionophore monensin and DCCD (Figure S6B). No significant ATP synthesis could be detected in the absence of Na+, even in the presence of a valinomycin-induced K+ diffusion potential. However, a chemical gradient of Na+ was not sufficient to drive ATP synthesis in the absence of a membrane potential. These data support the presence of a Na+-coupled F-type ATP synthase in membrane vesicles of F. nucleatum. We next purified the ATP synthase, ultimately achieving a 38-fold purification (Table S1). Silver-stained SDS-gels and MALDI were used to confirm that all constituent subunits were present in the purified sample (Figure S7). The ATPase activity of the purified enzyme was stable over several days (Figure S8A). The apparent Km for Mg2+ and ATP were 0. 64 mM and 0. 25 mM, respectively (Figure S8B–S8C). CaCl2 could not replace MgCl2, since no activity was detected at increasing concentrations of CaCl2 (unpublished data). Two cornerstone properties of all Na+-coupled ATP synthases are the ability to be specifically stimulated by low amounts of Na+ (or Li+), and the protective effect of Na+ against DCCD inhibition [22], [23]. Both these properties were apparent in the F. nucleatum ATP synthase (Figures 4 and 5). While K+ had no stimulatory effect on the ATPase activity, Na+ (and Li+) stimulated maximally at low millimolar concentrations (Figure 4A), both at neutral and high pH (Figure 4B). The ATPase activity of the enzyme was inhibited by DCCD in a concentration-dependent manner (Figure S9). However, even with high concentrations of DCCD (400 µM), 50 mM Na+ (or 50 mM Li+, but not K+) strongly protected the enzyme from inhibition (Figure 5). The protective effect of Na+ was most apparent at lower pH values, consistent with the fact that DCCD modification requires protonation of Glu65. These observations are strongly indicative of a specific binding site for Na+ in the c-ring of the F. nucleatum ATP synthase. We next purified the F. nucleatum c-ring using a novel heterologous expression system in Escherichia coli DK8 cells, which synthesizes a hybrid F1Fo-ATP synthase with subunits F1-ab2 from I. tartaricus and the c-ring from F. nucleatum. From this hybrid F1Fo complex we isolated the c-ring, which migrates at ∼51 kDa on an SDS-polyacrylamide gel, disintegrating into c-monomers only upon tricholoroacetic acid (TCA) treatment (Figure S10). The expected mass of F. nucleatum c-subunit was confirmed by mass spectrometry (Figure S11). To address the question of ion specificity, we exposed detergent-solubilized c-rings to N-cyclohexyl-N′- (4- (dimethylamino) -α-naphthyl) -carbodiimide (NCD-4), a fluorescent DCCD analogue, in the absence and presence of Na+ at pH 5. 7. The reaction of NCD-4 with the c-ring resulted in a continuous increase of fluorescence (Figure 6). The reaction stopped immediately upon addition of only 15 mM NaCl, but continued linearly in a separate long-term control experiment in the absence of NaCl (Figure S12). These data indicate that NCD-4 modifies Glu65 in the isolated c-ring, and that Na+ ions inhibit this reaction, in clear contrast to similar measurements for H+-coupled c-rings, in which Na+ had no effect [8], [10]. The rapid protective effect of Na+ demonstrates that ions can access the binding sites of detergent-solubilized c-rings in less than a few seconds (pipetting time), consistent with a previous report for an analog fluorescent inhibitor [24]. It is also worth mentioning that the migration of intact and monomerized (TCA-treated) c-ring samples on an SDS-polyacrylamide gel, exposed to UV light, was significantly reduced after NCD-4 modification (Figure S13), indicating that NCD-4 becomes covalently bound to the c-ring. To obtain structural evidence of a Na+-binding site in the F. nucleatum ATP synthase, we solved the atomic structure of its c-ring by X-ray crystallography. Crystals of the c11 ring were first grown at pH 5. 3 and one of the crystals yielded a complete 2. 2 Å dataset (Table S2). In this crystal, one asymmetric unit contains two complete c-rings. Like the c-ring from I. tartaricus [6], the F. nucleatum rotor is made by 11 identical c-subunits (Figure 7). Each c-subunit consists of two transmembrane α-helices connected by a short loop, which would face the cytoplasmic side of the membrane; both N- and C-termini would therefore be in the periplasmic space. In the crystal, lattice contacts are established between the hydrophilic C-termini and the short loop. The c11 ring is ∼68 Å in height and its outermost diameter, towards the cytoplasmic side, is ∼52 Å (Figure 7). The diameter of the ring is smallest at its middle (∼40 Å), i. e. , at the level of the ion-binding sites, and therefore its shape resembles an hourglass. The transmembrane region of the protein appears to extend from Tyr80 to Ser55 on the periplasmic and cytoplasmic sides, respectively (Figure 8A). Within these limits, the c-ring surface is highly hydrophobic, while polar and charged amino acids are predominantly found on the surface facing the cytoplasm, as well as at the N- and C-termini. Like other c-rings, the F. nucleatum c11 ring features an inner pore (Figure 7B), mostly lined by hydrophobic residues. Detergent molecules can also be discerned on the periplasmic entrance of the pore, suggesting that this pore would be blocked by phospholipids (Figure S2B) in vivo. According to the mass spectrometry analysis (Figure S11), a small fraction of the c-subunits in our preparation is formylated at the N-termini, but apparently not in sufficient amounts to yield enough electron density from the diffraction data. The c-ring was crystallized in the presence of near-physiological concentrations of Na+ (100 mM). To confirm the location of the Na+-binding sites, we calculated an omit electron-density map (Figure 7). This map reveals 11 binding sites, each located at one of the interfaces between c-subunits. A close-up view of these sites reveals an interaction network of several amino acids around the central density caused by Na+ (Figure 8B; Table 1). The arrangement of the Na+-binding site is highly similar to that in the I. tartaricus c11 ring [6], [9]. The most notable difference is the presence of a second glutamate side-chain, Glu32, in addition to the conserved Glu on the C-terminal helix (Glu65). Both side-chains contribute to Na+ coordination, via one oxygen atom of each carboxyl group. The other two oxygen atoms are at hydrogen-bonding distance of ∼2. 7 Å from each other (Table 1). This arrangement suggests that a proton is bound to the site in addition to Na+, consistent with the free-energy calculations described above (Figure 2). In addition to Glu32 and Glu65, Ser66: Oγ and Val63: O from the adjacent c-subunit also contribute to Na+ coordination. Ser66: Oγ and Tyr70: OH also interact with Glu65, as hydrogen-bond donors, stabilizing the so-called ion-locked conformation of the site [6]. Finally, electron density for a water molecule is found within coordinating distance from the ion, with Thr67: Oγ and Ala64: O as potential hydrogen-bond acceptors. We also investigated the influence of the environmental pH on the conformational state of the ion-binding site, by crystallizing the F. nucleatum c11 ring in NaOH-titrated buffer at pH 8. 7. The resulting structure, at 2. 6 Å resolution (Table 1), revealed again a locked conformation of the binding site identical to that at pH 5. 3, with Na+ and H+ bound concurrently (Figure 8). As we observed in previous structural studies of c-rings [10], [25], the detergent used in these crystals provides a hydrophobic belt that effectively uncouples the binding sites from the pH or Na+ concentration conditions in the crystallization buffer, in a manner that likely resembles the shielding effect of a lipid membrane. The finding that one Na+ and one H+ can concurrently occupy the binding sites in the F. nucleatum c11 ring raises the possibility that both ions are co-transported across the membrane as the ring rotates. This would imply that ATP synthesis in F. nucleatum is not only coupled to the SMF, but also to the PMF. More generally, the implication for other c-rings whose ion-binding sites include two carboxyl groups would be an ion-to-ATP ratio that is twice larger than what the c-subunit stoichiometry indicates (here 22/3 instead of 11/3). In the operating enzyme, ion loading and release from the c-ring occurs when each of the c-subunits encounters the interface with the adjacent subunit-a, as the ring rotates within the membrane. No high-resolution structural data are available for subunit-a or its interface with the c-ring, but this interface is reportedly well hydrated [26], [27]. We have previously shown that hydration is sufficient to facilitate the reversible gating of the c-subunit binding sites, and to enable binding and unbinding of ions [25], [28]. In the open, ion-free state, the conserved glutamate projects away from the ring [25], [28], [29] and is believed to transiently engage a conserved arginine side-chain in the fourth transmembrane helix of subunit-a [30], [31]. From an electrostatic standpoint, the likelihood of concurrent release of Na+ and H+ from the F. nucleatum ring would be maximal in this hypothetical transient state, since negatively charged Glu65 would be more distant from Glu32 than in the closed state, while still being neutralized by the key arginine in subunit-a, instead of the bound Na+. To clarify whether the likelihood of this event is significant, we created an idealized structural model of this intermediate configuration (Figure 9). We then re-computed the pKa of Glu32 using FEP simulations. As shown in Figure 2B, the upward shift in the calculated pKa of Glu32 in this open, hydrated state is indeed significantly smaller than in the closed state, but nevertheless deprotonation remains very unlikely. This result implies that only Na+ would be released from the c-ring, and therefore translocated across the membrane, while the additional H+ are constitutively bound to the structure. To validate this conclusion experimentally, we examined the extent of proton uptake into inverted membrane vesicles from F. nucleatum and E. coli cells, driven by their respective ATP synthases, functioning in the ATP hydrolysis direction (see Methods). As expected, addition of ATP to the E. coli membranes resulted in the quenching of the fluorescence pH reporter acridine orange, due to H+ accumulation inside the vesicles (Figure 10A). This quenching was reversed by the addition of CCCP demonstrating that a pH gradient was indeed present. However, no discernible quenching was detected for F. nucleatum, under conditions in which its ATP synthase is active in hydrolysis. Specifically we tested buffers with Na+ concentrations of ∼2 mM and 10 mM, at pH values of 6 (Figure 10B and 10C) or lower (unpublished data). Under these conditions we expect ATP hydrolysis to be coupled to Na+ uptake into the vesicles, based on the above mentioned activity and inhibition measurements for both the purified enzyme (Figures 4 and 5) and the c-ring (Figure 6), and the fact that ATP synthesis in these membrane vesicles is Na+-dependent and sensitive to monensin (Figure S6B). However, proton co-transport was not detected in any case. In sum, computations and measurements demonstrate that the protons that occupy the ion-binding sites in the F. nucleatum c-ring, concurrently with Na+, remain bound to the structure throughout the rotation of the ring, and therefore are not co-translocated with Na+ across the membrane.
  We describe the structure of a novel type of Na+-binding site in the c-ring of the F1Fo-ATP synthase from F. nucleatum and demonstrate that this enzyme is physiologically coupled to the SMF. The c-ring features two glutamate residues in each of its 11 ion-binding sites, instead of a single carboxylate side-chain as is typical. While such a motif has been observed in other Na+-binding proteins, and had been anticipated for c-rings in particular [9], [18], the implications in regard to the mechanism of ion transport and the specificity of the enzyme were unclear. A previous biochemical study of the PMF-driven ATP synthase from E. coli analyzed mutants with a two-carboxylate motif in the c-ring proton-binding sites, concluding that the engineered carboxylate (Asp24), which replaces a hydrophobic side-chain (Ala24), must be protonated for the enzyme to function, while it is the aspartate side-chain found in the wild-type enzyme (Asp61) that likely mediates H+ translocation [32]. The substitution of Ala24 by Asp or Glu, however, induces a pH dependence on the H+ transport activity in the physiological pH range, not observed for the wild-type enzyme. In a subsequent study of the two-carboxylate E. coli mutants, this dependence led to the proposal that the second carboxylate found in the binding sites of some c-rings might function as a regulatory high-pH sensor to inhibit the ion-translocation mechanism [18]. In light of our results, however, we interpret this pH dependence differently. In our view, it likely reflects a down-shift in the overall pKa of the proton-binding sites in the mutagenized E. coli c-ring, as a result of the constitutive protonation of the added carboxylic side-chain, which naturally diminishes the affinity for a second proton (i. e. , the one transported across the membrane), relative to the wild-type. This interpretation would also be consistent with the diminished rate of H+ translocation mediated by these two-carboxylate mutants. In support of our hypothesis that the second carboxylate group is constitutively protonated, we find that under physiological conditions the F. nucleatum ATP synthase mediates the translocation of Na+ only, i. e. , protons are not co-transported during either ATP hydrolysis or ATP synthesis, even though they are concurrently bound to the c-ring binding sites, as the crystal structures and calculations demonstrate. The structural basis for the ion specificity of the ATP synthase can be rationalized on the basis of the amino acid composition of the c-ring binding sites, and the concentrations of the two competing ions in the environment [8]. This study indicates that the F. nucleatum c-ring preferentially uses Na+ as the coupling ion, but only under conditions in which Na+ is in large excess over H+, e. g. , in physiological settings. The c-ring is in fact selective for H+, like all other rings characterized thus far. In particular it appears to be ∼100-fold more H+ selective than the ring of the Na+-driven V-type ATPase from E. hirae, which reportedly is almost non-selective [21]. The finding that the additional glutamate side-chain in the c-ring binding sites is constitutively protonated implies that its energetic contribution to ion selectivity is largely equivalent to that of a glutamine or asparagine side-chain at the same position. We expect this equivalence to hold true also for H+-driven ATP synthases with a double-carboxylate motif, such as those in Streptococcus pneumoniae [33] and M. tuberculosis (Figure S1). These c-rings probably bind two H+ concurrently to each site (as in Figure 3A), only one of which is likely to be transported. This perspective would also be consistent with a recent study of the ATP synthase from the archaeon Methanosarcina acetivorans [19], whose c-ring binding sites were also predicted to feature two glutamate side-chains, one of which was proposed to be constitutively protonated. The M. acetivorans ATP synthase is reportedly coupled to both Na+ and H+ translocation under physiological conditions [19]. However, this is not due to the deprotonation of the atypical carboxyl side-chain in the c-ring (the equivalent to Glu32 in F. nucleatum), but rather to the increased H+ specificity of its binding sites, relative to Na+-driven c-ring rotors (Figure 3B). Physiologically, i. e. , with Na+ in large excess over H+, the more pronounced H+ selectivity of the M. acetivorans c-ring makes the likelihood of a state with two bound H+ comparable to that with one Na+ and one H+, in contrast to F. nucleatum, for which the latter is the most probable. Intriguingly, all Na+-coupled c-rings from F-type ATP synthases characterized so far consist of 11 α-helical hairpins, including those of I. tartaricus, P. modestum, A. woodii, C. paradoxum [24], [34]–[36], and now F. nucleatum. The only apparent commonality of these bacteria is that they grow under anaerobic conditions and in environments where Na+ is abundant (e. g. , marine sediment). All these organisms thus use the SMF to sustain ATP production, but a phylogenetic relationship among them is not apparent. A more evident similarity, besides the near-identical amino acid composition of the Na+-binding sites, is a glycine-rich motif in the N-terminal helix of the c-subunit (Figure S1), which has been shown to have a strong influence on the stoichiometry of the c-ring [14], [37], [38]. It would be therefore tempting to hypothesize that the 11-hairpin architecture might reflect a universal feature of all Na+-driven c-rings in F-type ATP synthases. Nevertheless, studies of A- and V-type rotary ATPases indicate that Na+ specificity is compatible with other c-ring stoichiometries [20], [39], [40]. Consistently, single-point mutations of the glycine-motif in the c11 ring from I. tartaricus result in increased c-subunit stoichiometries, but do not necessarily impair Na+ coupling [14]. Further experimental data will be required to clarify this question conclusively. In recent years, the ATP synthase has come into focus as a novel drug target [41]. Of particular interest is the finding that the M. tuberculosis c-ring can be inhibited by diarylquinolines, a new class of antibiotic compounds against multi-drug resistant strains [42], [43]. Even in non-replicating or persister cells of M. tuberculosis, the PMF is obligatory for ATP homeostasis [44] and hence it is of medical interest to develop strategies to disrupt energy generation via the ATP synthase. This notion also holds true for F. nucleatum, which is a pervasive bacterium in dental plaque biofilms, and which is associated with periodontitis, one of the most common human infections [45]. The fact that the c-ring of the F. nucleatum ATP synthase is Na+-coupled and that its structure is significantly different from mitochondrial c-rings [13], [28] opens an opportunity for new selective drugs against this opportunistic human pathogen.
  Molecular dynamics (MD) simulations and free-energy calculations were based on a homology model of the F. nucleatum c11 ring embedded in a phospholipid membrane (Figure S2) as described previously [46]. The Free-Energy Perturbation (FEP) method was employed in all ion-selectivity and pKa calculations, as implemented in NAMD 2. 7 [47]. Ab initio calculations were carried out with Gaussian09 (Frisch M. J. et al. , Gaussian Inc. , Wallingford CT, 2009) at the HF/6-31G* level. Further details see Text S1. F. nucleatum, subsp. nucleatum ATCC25586 was grown anaerobically at 37°C in Columbia broth (Difco) supplemented with cysteine-hydrochloride (reductant) and resazurin as an oxygen indicator (pH 7. 5). Cells were routinely grown in either anaerobic N2/H2/CO2 (90∶5∶5) -gassed Hungate tubes or in 1 l Schott bottles. For further details see Text S1. Cells grown to mid-exponential phase were passed three times through a French press cell (20,000 psi) and the emulsion was centrifuged (8,000 g for 10 min) to remove cell debris. Inverted membrane vesicles were collected by ultracentrifugation, washed twice, and adjusted to a final protein concentration of 20–30 mg/ml. For further details see Text S1. Inverted membrane vesicles were solubilized in reducing buffer (pH 7. 5) using 2% (w/v) dodecylmaltoside, 10% (v/v) glycerol, and a protease inhibitor cocktail at 4°C for 1 h. The solubilizate was further purified by anion exchange chromatography and the ATP synthase-containing fractions were concentrated by ultrafiltration. The sample was further purified by gel filtration, desalted, and analyzed by SDS-PAGE. The identification of the F1Fo-ATP synthase subunits was further confirmed by mass spectrometry (MALDI TOF/TOF MS). ATP hydrolysis activity was measured using the spectrophotometric ATP-regenerating assay or via the release of Pi. ATP-driven proton translocation was determined by the quenching of acridine orange (AO), as described previously [48] with some modifications as described in Text S1. ATP synthesis in inverted membrane vesicles was determined via the standard luciferin-luciferase system, monitoring the light emitted with a chemiluminometer (FB 12 luminometer; Berthold) at 37°C, as described before [49]. For further details see Text S1. The F. nucleatum c11 ring was produced in a heterologous hybrid ATP synthase expression system [50] forming the I. tartaricus ATP synthase (F1ab2) and the F. nucleatum c11 ring. The purification of the c-ring was performed by a procedure as described in Text S1. NCD-4 labeling experiments were performed as previously [24] with slight modifications described in Text S1. Crystallization of the F. nucleatum c11 ring was performed by vapor diffusion in hanging drops at 18°C. The crystals were flash-frozen in liquid nitrogen. For further details see Text S1. Data to 2. 2 Å (pH 5. 3) and 2. 64 Å (pH 8. 7) were collected at the Max-Planck/Novartis beamline X10SA (PXII) of the Swiss Light Source (SLS, Villigen, Switzerland) and the beamline ID23. 2 of the European Synchrotron Radiation Facility (ESRF, Grenoble, France), respectively. Data processing and structure modeling was performed as described in Text S1. The atomic coordinates and structure factors of the F. nucleatum c11 ring at pH 5. 3 and pH 8. 7 were deposited in the Protein Data Bank, under the accession numbers 3ZK1, and 3ZK2, respectively.
summary: Essential cellular processes such as biosynthesis, transport, and motility are sustained by the energy released in the hydrolysis of ATP, the universal energy carrier in living cells. Most ATP in the cell is produced by a membrane-bound enzyme, the ATP synthase, through a rotary mechanism that is coupled to the translocation of ions across the membrane. The majority of ATP synthases are energized by transmembrane electrochemical gradients of protons (proton-motive force), but a number of organisms, including some important human pathogens, use gradients of sodium ions instead (sodium-motive force). The ion specificity of ATP synthases is determined by a membrane-embedded sub-complex, the c-ring, which is the smallest known biological rotor. The functional mechanism of the rotor ring and its variations among different organisms are of wide interest, because of this enzyme' s impact on metabolism and disease, and because of its potential for nanotechnology applications. Here, we characterize a previously unrecognized type of Na+-driven ATP synthase from the opportunistic human pathogen Fusobacterium nucleatum, which is implicated in periodontal diseases. We analyzed this ATP synthase and its rotor ring through a multi-disciplinary approach, combining cell-growth and biochemical assays, X-ray crystallography and computer-simulation methods. Two crystal structures of the membrane rotor were solved, at low and high pH, revealing an atypical ion-recognition motif mediated by two carboxylate side-chains. This motif is shared by other human pathogens, such as Mycobacterium tuberculosis or Streptococcus pneumonia, whose ATP synthases are targets of novel antibiotic drugs. The implications of this ion-recognition mode on the mechanism of the ATP synthase and the cellular bioenergetics of F. nucleatum were thus examined. Our results provide the basis for future pharmacological efforts against this important pathogen.
keywords: macromolecular complex analysis
  biochemistry
  microbial physiology
  macromolecular assemblies
  protein structure
  bioenergetics
  biology
  computational biology
  microbiology
  biophysics simulations
  biophysics
  bacterial pathogens
  drug discovery
  macromolecular structure analysis
title: A New Type of Na+-Driven ATP Synthase Membrane Rotor with a Two-Carboxylate Ion-Coupling Motif