article: Eukaryotic cells must inhibit re-initiation of DNA replication at each of the thousands of origins in their genome because re-initiation can generate genomic alterations with extraordinary frequency. To minimize the probability of re-initiation from so many origins, cells use a battery of regulatory mechanisms that reduce the activity of replication initiation proteins. Given the global nature of these mechanisms, it has been presumed that all origins are inhibited identically. However, origins re-initiate with diverse efficiencies when these mechanisms are disabled, and this diversity cannot be explained by differences in the efficiency or timing of origin initiation during normal S phase replication. This observation raises the possibility of an additional layer of replication control that can differentially regulate re-initiation at distinct origins. We have identified novel genetic elements that are necessary for preferential re-initiation of two origins and sufficient to confer preferential re-initiation on heterologous origins when the control of re-initiation is partially deregulated. The elements do not enhance the S phase timing or efficiency of adjacent origins and thus are specifically acting as re-initiation promoters (RIPs). We have mapped the two RIPs to ∼60 bp AT rich sequences that act in a distance- and sequence-dependent manner. During the induction of re-replication, Mcm2-7 reassociates both with origins that preferentially re-initiate and origins that do not, suggesting that the RIP elements can overcome a block to re-initiation imposed after Mcm2-7 associates with origins. Our findings identify a local level of control in the block to re-initiation. This local control creates a complex genomic landscape of re-replication potential that is revealed when global mechanisms preventing re-replication are compromised. Hence, if re-replication does contribute to genomic alterations, as has been speculated for cancer cells, some regions of the genome may be more susceptible to these alterations than others.
  The initiation of eukaryotic DNA replication is tightly regulated so that it occurs at most once per cell cycle [1]. This regulation is critical because re-replication of a chromosomal segment makes that segment highly susceptible to genomic alterations [2]. Preventing re-replication throughout the genome is particularly challenging for eukaryotic cells because their genomes contain hundreds to thousands of replication origins. Hence, each individual origin must be tightly controlled if a genome is to avoid any re-initiation events [3]. The basic strategy eukaryotic cells use to prevent re-initiation is to prevent the reassembly of replication initiation complexes at origins that have fired. The critical assembly step that is regulated is the loading of the core replicative helicase Mcm2-7, which forms a toroidal complex that encircles the origin DNA [4]. This loading is carried out by four factors: the origin recognition complex (ORC), Cdc6, Cdt1, and Mcm2-7 [5], [6]. In the budding yeast, Saccharomyces cerevisiae, cyclin dependent kinases (CDKs) use multiple mechanisms targeting each of these proteins to prevent the reloading of Mcm2-7 once cells enter S phase [7]. In other organisms, additional CDK-independent mechanisms have been identified that inhibit Cdt1. The precise mechanisms used differ among species, but the reliance on multiple mechanisms targeting each of the initiation proteins involved in Mcm2-7 loading is highly conserved [3], [5]. The paradigm that has thus developed for the cell cycle control of replication initiation is that a multitude of overlapping mechanisms collaborate to globally inhibit initiation proteins throughout the cell, thereby minimizing the odds of re-initiating at any origin [8]. Consistent with this paradigm, disruption of individual mechanisms often does not lead to measurable re-replication even though the suspected consequences of re-replication, e. g. DNA damage or genomic alterations, have been observed [9]–[11]. Therefore, any investigation into the role of individual regulatory mechanisms in the block to re-initiation must be conducted in a sensitized system where a number of other overlapping mechanisms have been disrupted and re-replication can be readily detected. Development of such sensitized systems revealed that origins re-initiate with diverse efficiencies and challenged the implicit assumption that all replication origins are uniformly regulated by global inhibition mechanisms [12], [13]. For example, when ORC, Cdc6, and Mcm2-7 are deregulated, many (∼100) origins detectably re-initiate, but many more (∼200) do not. Moreover, the amount of re-initiation from each origin varies widely. This diversity of re-initiation efficiency does not correlate with the diversity of S-phase origin timing and efficiency and thus cannot be explained by the chromosome context effects that are responsible for the latter [14]. Instead, the diversity in re-initiation efficiencies suggests that origins are not solely and uniformly regulated by global controls. Thus, we believe the paradigm for re-initiation control needs to be modified by the addition of a local layer of control that can modulate how tightly the global regulatory mechanisms inhibit re-initiation at specific origins. Here, we explore the workings of this local control by asking why some budding yeast origins re-initiate more readily than others when global restrictions on re-initiation are partially inactivated. We show that local sequence elements adjacent to these origins specifically promote their re-initiation without enhancing their initiation activity. Furthermore, these elements act independently of the chromosomal context and silencing effects that regulate S-phase origin timing and efficiency. These elements, which we term re-initiation promoters (RIPs), map to ∼60 bp segments that work in a distance- and sequence- dependent manner. Analysis of the re-association of Mcm2-7 with origins suggests that these RIP elements antagonize an inhibitory mechanism that operates after Mcm2-7 association with origins. These findings provide our first insight into how diversity can be introduced in the regulation of eukaryotic replication origins.
  To investigate the mechanisms underlying the diversity of origin regulation in the block to re-initiation, we examined S. cerevisiae origins whose ability to escape this regulation stood out the most from other origins. We previously reported that re-initiation occurs predominantly from ARS317 in a strain where a subset of global replication controls was disrupted [12]. This “MC2Ao” strain was deregulated in three ways: (1) (M) - the CDK driven export of Mcm2-7 from the nucleus [15]–[17] was blocked by fusing a constitutive nuclear localization signal onto the endogenously expressed Mcm7; (2) (C2A) – the CDK inhibition of Cdc6, which occurs through transcriptional regulation [18], phosphorylation-directed degradation [8], [19], [20], and direct CDK binding [21], was completely disrupted by expressing an extra copy of Cdc6 lacking CDK phosphorylation and binding sites under a galactose-inducible promoter; and (3) (o) - the CDK inhibition of ORC by phosphorylation of Orc2 and Orc6 was minimally perturbed by eliminating one of four CDK consensus phosphorylation sites on Orc6 [2]. We note that this ORC deregulation was not necessary for the preferential re-initiation of ARS317, but enhanced it approximately 3-fold (Figure S1). Importantly, of the known mechanisms preventing re-initiation in budding yeast, two are retained in this strain: (1) CDK phosphorylation of Orc2 and Orc6 (9 out of 10 CDK consensus phosphorylation sites remain unmutated) [7]; and (2) Clb5-Cdc28 binding to an RXL docking site on Orc6 [22]. Re-initiation was not detectable in the MC2Ao strain until the deregulated Cdc6 was induced. We could thus arrest cells at metaphase with a normal 2C DNA content across the genome, induce the deregulated Cdc6, and detect re-initiation and re-replication as a >2C DNA copy number using array comparative genomic hybridization (aCGH). Although the primary re-initiation event after a 3 hr induction of re-replication was at ARS317 [12], the re-replication profiles showed hints of additional re-replication peaks at other genomic loci. At least two of these peaks were readily confirmed with a longer 6 hr induction of re-replication, one on the right arm of Chr 5 near position 575 kb, and one on the right arm of chromosome 12 near position 890 kb (Figure 1A). The latter was dependent on ARS1238, establishing that this origin also preferentially re-initiated in the MC2Ao strain (Figure 1B). Because ARS317 and ARS1238 were among the two most efficient re-initiating origins, we focused on them to investigate why some origins are more susceptible to re-initiation than others. We first sought to determine whether the preferential re-initiation of ARS317 and ARS1238 was conferred by the origin and immediate surrounding sequences or required a broader chromosomal context that spans kilobases of DNA. An example of the latter is the poorly understood chromosome position effect that has been implicated in the diversity of yeast origin timing and efficiency during normal S phase initiation (discussed in [14], [23]). We and others had previously shown that there was no correlation between this diversity of origin activity in S phase and the diversity of re-initiation efficiency displayed in strains where many origins re-initiate due to complete deregulation of ORC, Mcm2-7, and Cdc6 [12], [13]. Nonetheless, a different chromosomal context could be conferring preferential re-initiation on ARS317 or ARS1238 in the MC2Ao strain. To distinguish between local sequence determinants and a broader chromosomal context, we investigated whether small fragments containing the ARS317 or ARS1238 origins could preferentially re-initiate when transplanted to ectopic genomic loci. We focused initially on fragments that we hoped would be small enough to dissect at the nucleotide level but large enough to encompass the origin and any possible additional sequences that might be needed for preferential re-initiation. A 537 bp fragment previously shown to contain ARS317 [24] preferentially re-initiated when transplanted from its endogenous location to sites on other chromosomes (ChrIV_567 kb, ChrIV_1089 kb) [2], [12]. In all cases, the amount of re-initiation induced after 3 hr (2. 7–3. 0 C) at the ectopic locus was comparable to the amount of re-initiation at the endogenous locus (2. 8–3. 2 C) [2], [12]. Hence, neither the chromatin context nor the replication timing (early or late in S-phase) of the transplant location were key determinants of the re-replication activity on these origins. Consistent with this notion, Figure 2A shows that an even smaller 406 bp fragment containing ARS317 preferentially re-initiates when transplanted to position ChrIV_567 kb. At this same location, a 233 bp ARS1238 fragment that contains the ORC binding site (OBS) and 100 bp of flanking sequence on either side [25] also re-initiates (Figure 2A). Thus, the preferential re-initiation of ARS317 and ARS1238 is conferred by local sequence determinants and is independent of a broader chromosomal context. ARS317 is a core element of a 138 bp transcriptional silencer HMR-E, one of several silencers that recruit the silencing proteins Sir1-4 to organize the surrounding DNA into a heterochromatin-like structure (reviewed in [26]). The entire HMR-E silencer is included within the transplanted ARS317-containing fragments described above, so the preferential re-initiation of this fragment could be associated with its organization into heterochromatin [27], [28]. Such a connection is reminiscent of reports that heterochromatin preferentially re-replicates in budding yeast and Drosophila [13], [29]. To test this possibility, we individually deleted each of the four SIR genes and analyzed the re-replication profiles around ARS317 for each sir mutant. These profiles resembled those from the wild-type SIR control strains (Figure 2B and Figure S2A), indicating that none of the Sir proteins are required for the preferential re-initiation of ARS317. We also observed re-replication in a truncated ARS317 clone lacking the RapI and AbfI binding sites that are critical for HMR-E silencer function [28] (Figure 2C). We conclude that a silent chromatin state is not necessary for the preferential re-initiation of ARS317. ARS1238 is not assembled into heterochromatin, so one would expect its preferential re-initiation to be independent of Sir proteins. Our data are consistent with this expectation (Figure S2C), although the profiles are not as clear-cut. Other factors known to influence nearby origin function are the forkhead transcription factors Fkh1 and Fkh2. Association of these proteins with origins and ORC has been implicated in the spatial organization of origins in the nucleus. This organization is thought to alter the S phase replication timing of some origins, including ARS1238 [30]. Although Fkh proteins do not influence ARS317 replication timing, searches for their proposed binding motifs have identified predicted binding sites within a few kilobases of both ARS317 and ARS1238 [31], [32]. To test whether Fkh1 or Fkh2 are critical for re-initiation of either origin, we examined the re-replication profiles in fkh1Δ, fkh2Δ, and fkh1Δfkh2Δ strains. At both ARS317 (Figure 2D and Figure S2B) and ARS1238 (Figure S2D), fkhΔ strains re-replicated significantly more than negative control strains that lack re-replicating origins at these loci. These results confirm that the forkhead proteins are not essential for the preferential re-initiation of either origin. We did observe a partial reduction of re-replication in the fkh1Δfkh2Δ background, so we cannot rule out a role for these proteins in supporting re-initiation. However, we suspect that this reduced re-replication may be an indirect consequence of the severe growth defect and cell clumping exhibited by the double mutant during growth in liquid media [30]. The preferential re-initiation activity seen in transplanted fragments containing ARS317 and ARS1238 could be intrinsic to the origin sequences themselves, or be conferred on these origins by neighboring sequences that are dispensable for initiation activity. The former possibility is particularly relevant for ARS317, whose especially tight interaction with ORC appears to govern the activity of this origin in S phase [33]. If this possibility is correct, any minimal segment containing origin activity should also exhibit preferential re-initiation. In contrast, if the latter possibility is correct, the fragments should be separable into an origin segment that can initiate but not preferentially re-initiate, and an adjacent segment that can neither initiate nor preferentially re-initiate on its own but confers preferential re-initiation on the origin segment. To test this separability, of functions for both ARS317 and ARS1238 we generated subclones of the transplanted fragments described in Figure 2A and assayed them for both initiation and re-initiation activity. Initiation activity requires a 33 bp consensus ORC binding site (OBS) and less well-defined flanking sequences [34], [35]. The OBS is comprised of a 17 bp extended ARS consensus sequence (eACS), formerly known as the A domain, and a WTW sequence [36] formerly known as the B1 subdomain. The required flanking sequences usually lie 3′ of the T-rich strand of the OBS, where they comprise the rest of the B domain (B2 and B3), but occasionally can lie 5′ of the OBS, where they are referred to as C domain sequences [37]. We numbered nucleotides in our subclones relative to the OBS [35], assigning +1 and +33 to the first and last nucleotide, respectively, of the T-rich strand of the OBS. In this scheme, B domain sequences outside the OBS are numbered +34 and higher, and C domain sequences have negative coordinates (Figure 3A). The 406 bp preferentially re-initiating fragment containing ARS317 is thus designated 317 (+300. . -106), and the equivalent 233 bp fragment for ARS1238 is designated 1238 (+133. . -100). The initiation activity of an origin can be assayed by the ability of a plasmid containing the origin to be maintained in cells. One measure of this ability is the mitotic stability assay, which measures the steady state percentage of cells containing the plasmid in a culture grown under selection for the plasmid [38], [39]. The mitotic stability of several subfragments containing ARS317 showed that full origin activity was retained by 317 (+76. . −106) (Figure 3B). This origin segment failed to re-initiate when inserted at ChrIV_567 kb (Figure 3C), demonstrating that ARS317 does not have an intrinsic ability to re-initiate. The adjacent segment 317 (+300. . +77) was also not able to re-initiate when examined in the context of a slightly larger fragment 317 (+300. . +34) at ChrIV_567 kb (Figure 3D). This adjacent segment does contain sequences that are essential for a weak cryptic origin (Figure 3A) [36], but a mutation that disrupts this cryptic origin did not reduce the ability of these adjacent sequences to induce re-initiation (Figure S3; mutant A). In contrast, a mutation of the ARS consensus sequence in the ARS317 OBS did eliminate re-initiation, confirming that the re-initiation is dependent on ARS317 ([12], also Figure S3 mutant E). These data show that the preferentially re-initiating fragment 317 (+300. . −106) can be separated into an ARS317 origin segment 317 (+76. . −106) and an adjacent segment 317 (+300. . +77) that confers preferential re-initiation on ARS317 in the MC2Ao strain. We call the sequence element that confers this activity a re-initiation promoter (RIP) and will refer to it as RIP317. We used a similar approach to identify a subsegment of 1238 (+69. . −100) that retains full ARS1238 origin activity (Figure 3B) but is not sufficient to preferentially re-initiate. This inability to re-initiate was demonstrated in the context of a slightly larger segment 1238 (+83. . −100) at ChrIV_567 kb (Figure 3E). Further evidence that neither origin segment nor adjacent segment have re-initiation activity on their own comes from insertion mutations (discussed later) that separate the two segments by 153 bp, and abolish re-initiation. In addition, the adjacent segment 1238 (+133. . +70) does not contain the origin activity needed to support maintenance of an autonomous plasmid. Thus, like ARS317, ARS1238 acquires its ability to preferentially re-initiate from an adjacent re-initiation promoter, which we will refer to as RIP1238. In order to map RIP317 with finer resolution, we first analyzed the re-initiation efficiency of a nested series of deletions extending into the left border (plus side) of the 406 bp 317 (+300. . −106) fragment. These deletion constructs were introduced into ChrIV_567 kb, and their re-initiation efficiency measured by normalizing the amount of re-initiation for each deletion (i. e. the copy number increase above 2C) against the amount of re-initiation for the full-length fragment. Deletions up to nucleotide +153 had limited effect on re-initiation efficiency, but further deletion into the fragment caused a precipitous drop (Figure 4A). Thus, nucleotide +153 in the 259 bp deletion fragment 317 (+153. . −106) defines a left-hand boundary for RIP317. To further map RIP317 we used 317 (+153. . −106) as the parent sequence for a linker scan analysis of RIP317 structure (Figure 4A; bold line). Most of the linker mutations that showed a noticeable reduction in ARS317 re-initiation efficiency were from L4 to L15, which covers the 51 bp from nucleotide +137 to +87 (Figure 4B). On the left end of this 51 bp region were linker mutations (L4–L7), which drastically reduced or eliminated ARS317 re-initiation and identified sequences that are critical for RIP function. Other linker mutations (L8–L15) showed less striking reductions in re-initiation individually (Figure 4B), but eliminated re-initiation when combined together (Figure S4A). Thus, the sequences mutated by linkers L8–L15 are also important for RIP function but may contain partially redundant sequence elements. In contrast to linker mutations L4–L15, the remaining linker mutations from L16–L32 each had limited effects on ARS317 re-initiation (Figure 4B). We note that ARS317 differs from most yeast origins in that the WTW sequence of its OBS is dispensable for initiation activity [36], [40]. Linker L29, which mutates the WTW sequence, and linkers L30 and L31, which intrude further into the OBS, still leave intact the 17 bp extended ARS consensus sequence (eACS), which forms the core of the ORC binding site [41]. Thus, although these linkers mutate parts of the OBS, they presumably do not disrupt ARS317 re-initiation efficiency because they leave ARS317 origin activity intact. Linker L33, on other hand, does mutate part of the eACS, so its partial disruption of ARS317 re-initiation is likely due to impairment of origin function. Replacement of the entire sequence covered by L17–L31 (nucleotides +86 to +23) with sequence of similar AT content did not have much effect on ARS317 re-initiation (Figure S4B). Additional replacement of sequences covered by L1–L3 decreased re-initiation efficiency by a third, indicating that these sequences contribute to optimal RIP317 activity (Figure S4B). These results suggest that RIP317 resides in the 67 bp from nucleotides +153 to +87 and contains a core region of approximately 19 bp (+137 to +119) that is crucial for its function. As discussed above, we had narrowed down RIP1238 to a 64 bp segment from nucleotide +133 to +70. Linker scan analysis revealed that linker mutations spanning 40 bp (+117 to +78) of this segment abolished ARS1238 re-initiation, while the remaining mutations showed a more modest reduction (Figure S4C). Thus, like RIP317, RIP1238 has a core segment that is crucial for RIP function and surrounding sequences that enhance this function. The most obvious common feature of RIP317 and RIP1238 is the high AT-content of these sequences (92% and 84% AT respectively). Regions of high AT-content have been postulated to exclude nucleosomes (Reviewed in [42]) or to provide regions of reduced helical stability that facilitate DNA unwinding during replication initiation [43]. Therefore, we wondered if RIP elements were stimulating re-initiation through such a positioning or thermodynamic mechanism. To test this possibility we generated various mutants that preserved the AT content of RIP317 while altering its sequence identity. Neither predicted nucleosome exclusion [44] nor predicted DNA helical stability [45] of RIP317-ARS317 is changed by these mutations. These mutations profoundly compromised re-initiation activity, with many of the mutants showing no re-initiation even after 6 hours of induction (Figure S5). These findings suggest that RIP elements do not simply act as a DNA unwinding element or a nucleosome exclusion site. We do note that many of the mutations disrupted a palindrome in RIP317 (5′-TTTATAAA-3′) that is also present in shorter form in RIP1238 (5′-TTATAA-3′). However, the palindrome in RIP1238 is not necessary for RIP function (Figure S4C, mutant B), and the palindrome in RIP317 is not sufficient (Figure S5, mutant D2). Thus, although our mutational data does not rule out a role for the palindrome that is specific for RIP317, the sequence dependence we observed is consistent with the RIP acting as a recruitment site for factors that promote re-initiation. The origin proximal boundary of RIP317 is 53 bp away from the B-side boundary of the ARS317 OBS. To determine whether the size of this spacing is important for RIP317 function, RIP-OBS spacing was increased by inserting randomly generated DNA of 38% AT-content (the average AT-content of genomic DNA in S. cerevisiae) between RIP317 and ARS317 and decreased by deleting portions of ARS317 in this 53 bp spacing (See Materials and Methods and Table S1). The resulting clones were analyzed for re-initiation efficiency (Figure 5A). Re-initiation declined with increased spacing and was abolished by 153 bp, suggesting that RIP317 must be relatively close to the origins to confer preferential re-initiation. Re-initiation could tolerate a decline in spacing to 37 bp but was significantly reduced by a spacing of 21 bp. The latter reduction, however, could simply be a secondary consequence of excessive removal of the B domain, which lies in the 53 bp spacing. Nonetheless, the overall finding is that re-initiation requires close but not precise spacing (within ∼35 to ∼75 bp) between the RIP and the OBS. A spacing of only 36 bp separates RIP1238 from the OBS of ARS1238. This short spacing suggested that ARS1238 might re-initiate less efficiently than ARS317 because the spacing is suboptimal. We thus performed a similar analysis of the spacing requirements between RIP1238 and the OBS of ARS1238 (Figure 5B). Like ARS317, re-initiation of ARS1238 also required relatively close spacing of the RIP and OBS (∼25 to ∼55 bp). Moreover, re-initiation levels were relatively constant across this range of spacings, indicating that the lower levels of re-initiation for ARS1238 versus ARS317 cannot be attributed to suboptimal RIP-OBS spacing for the former. This requirement for close proximity between RIP and origin raise the possibility that proteins bound to both sites must closely interact in some manner to facilitate re-initiation. If the RIP elements promote preferential re-initiation by influencing common regulatory pathways controlling origins, they should be able to promote re-initiation from heterologous origins. To test this possibility, we fused RIP317 and RIP1238 to other replication origins, keeping the spacing between RIP and origin OBS between 46–53 bp, within the optimal range of spacing determined for both ARS317 and ARS1238. These RIP-origin chimeras were then assayed at ChrIV_567 kb for re-initiation in an MC2Ao strain. RIP317 promoted preferential re-initiation from ARS1021 and ARS301 (Figure 6A) at levels comparable to the re-initiation it promoted from ARS317 (Figure 2D) following a 3 hr induction of re-replication (2. 8–3C), while fusions to a non-functional rip317 (equivalent to Figure 4B linker 6) failed to re-initiate. RIP317 also stimulated re-replication from ARS305, ARS209, and ARS1238, but a longer 6 hr induction of re-replication was needed to show an unequivocal stimulation (Figure S6A and S6B). RIP1238 was similarly able to promote preferential re-initiation from ARS1021 and ARS301 (Figure 6B). In these cases the re-initiation levels (4-4. 5C) were comparable to the re-initiation RIP317 promoted at ARS317 following a 6 hr induction of re-replication (compare to Figure 1). Thus, both RIPs can promote preferential re-initiation on heterologous origins. We did observe some origins (ARS306, ARS702) that exhibited no detectable preferential re-initiation when fused to RIP317 (Figure S6B). One possible reason is that the optimal spacing between the origin OBS and the RIP element places constraints on the size of the B domain that can fit between these two elements. Origins requiring larger B domains would be expected to have their initiation, and thus any re-initiation, compromised in their corresponding RIP fusion constructs. Consistent with this explanation, the truncated ARS306 and ARS702 fragments fused to RIP317 displayed defective origin function when assayed by plasmid mitotic stability (Figure S6C). Just as compromising origin function can reduce re-initiation efficiency, one can imagine that RIP elements might promote re-initiation by simply enhancing the intrinsic initiation efficiency of an origin. Such an effect was difficult to detect by plasmid mitotic stability because origins that re-initiate when fused to RIP317 (ARS317, ARS1021, and ARS301) appear to have maximal mitotic stability in this assay (Figure 7A). However, when integrated in the chromosome, ARS317, ARS1021, and ARS301 exhibited much lower initiation activity, allowing us to look for stimulation of this activity by RIP317. We used array CGH analysis of S phase replication to assay the activity of these origins with, and without, a functional RIP317 element. In the resulting replication profiles, the heights of the peaks represent a combination of the efficiency and timing of origin initiation in S phase. Low but measurable peak heights for the origins are ideal, because they leave open the maximal dynamic range for detecting a stimulation of origin activity by RIP317. We observed no measurable difference in replication peak heights for ARS317, ARS1021, and ARS301 with or without a functional RIP317 (Figure 7B). At its endogenous location ARS317 initiates in approximately 10–15% of cells each S phase based on 2-dimensional gel analysis of initiation bubble intermediates [46], [47]. Such origin activity at ChrIV_567 kb would be at the limit of detection for our aCGH replication assay, and any significant RIP317 stimulation of ARS317 activity should have been detectable as a larger peak. More striking is the detection of clear origin activity from ARS1021 and the absence of any stimulation of this activity from RIP317. These results argue that RIP317 does not advance the timing or enhance the initiation efficiency of adjacent origins. We thus favor a model in which RIP elements specifically promote re-initiation by antagonizing a mechanism (s) that prevents re-initiation. In vitro studies have shown that the loading of Mcm2-7 at origins can be subdivided into a sequence of discrete steps: (1) binding of ORC to origins; (2) recruitment of Cdc6 to ORC; (3) recruitment of Cdt1-Mcm2-7 to ORC-Cdc6; and (4) loading of a double hexamer of Mcm2-7 as a ring around the duplex origin DNA [48]. The numerous global mechanisms used by CDKs to prevent Mcm2-7 loading are thought to inhibit one or more of these steps, because once Mcm2-7 loading is complete, origins are primed to be activated by CDKs [49]–[51]. The partial deregulation of these mechanisms in the MC2Ao strain presumably allows some but not all of these steps to proceed, accounting for why the majority of origins do not re-initiate. RIP elements could therefore function by locally releasing an origin from the remaining block (s), allowing the origin to complete a re-initiation cycle. Thus, to gain insight into the mechanism of RIP action, we investigated which step in the loading process was blocked for the majority of origins that do not re-initiate in MC2Ao strains. We examined Mcm2-7 ChIP association at three origins that do not re-initiate in MC2Ao strains: ARS305, ARS418, and ARS1420. As expected, Mcm2-7 associated more with these origins relative to nonspecific DNA in G1 phase (Figure 8B) but not in M phase (Figure 8C). After a 90 minute induction of re-replication, Mcm2-7 became enriched 2–4× at these origins but not at a non-origin locus ACT1 (Figure 8D). ChIP also detected a similar degree of re-replication-induced association of Mcm2-7 with the two re-initiating origins, ARS317 and ARS1238 (Figure 8D). As expected, given the association of Mcm2-7 with origins that cannot re-initiate, preventing re-initiation of ARS317 by disrupting its adjacent RIP317 did not prevent the association of Mcm2-7 with ARS317 (Figure 8D). On the other hand, disrupting the ORC binding site in ARS317, did lead to loss of Mcm2-7 association, specifically with this origin (Figures 8C, 8D). This result is consistent with the in vitro dependence of Mcm2-7 origin association on ORC binding [52]. Taken together, our data indicate that the global deregulation of re-initiation in the MC2Ao strain allows Mcm2-7 to associate with most origins. Thus, in this strain the RIP elements must promote re-initiation at adjacent origins by facilitating or deregulating a step that is blocked after this association. As discussed below, determining more precisely which step is involved will require better in vivo tools to distinguish between the two types of association (Mcm2-7 recruitment versus loading) that have been identified in vitro.
  Preventing re-initiation at the hundreds to thousands of replication origins in a eukaryotic genome is critical for preserving genome stability [2]. Models for how such tight regulation can be achieved emphasize the importance of using numerous overlapping inhibitory mechanisms to reduce the probability that any origin will re-initiate [3], [8]. These mechanisms all inhibit the loading of the Mcm2-7 core replicative helicase onto origins, and each does so by reducing the total cellular activity of one of the four proteins required for this step: ORC, Cdc6, Cdt1, or Mcm2-7 [5], [6]. Given their global nature, these regulatory mechanisms are presumed to act equally at all origins throughout the genome. Thus, current models cannot account for the broad range of efficiencies with which origins re-initiate when global mechanisms are compromised. This diversity suggests that the models may be missing the contribution of local factors that can modulate the regulation of individual origins. Our work here demonstrates that such a local layer of regulation does indeed exist by identifying a local control that makes ARS317 and ARS1238 more susceptible to re-initiation when global regulation of Cdc6 and Mcm2-7 is removed. Our analysis of this control establishes some of its key mechanistic properties and constraints. First, this control specifically enhances the propensity of an origin to re-initiate and not its efficiency or timing during normal S phase initiation. Second, this preferential re-initiation is not imposed by a diffuse chromosomal context but is conferred by discrete sequence elements that are adjacent to but distinct from the origin. Third, these elements, which we call re-initiation promoters (RIPs), have specific sequence requirements and function best within a narrow range of distances close to the origin. Finally, these RIPs appear to overcome inhibitory mechanisms that block a step in initiation that follows the association of Mcm2-7 with origins. These results provide a paradigm for the local control of origin re-initiation and lay the groundwork for a more detailed molecular analysis of this control. Our results do not address the question of whether the presence and activity of these RIPs is incidental to some other genomic function of these elements or whether they arose for the purpose of modulating replication control in cells with intact replication controls. Nonetheless, as discussed below, the existence of RIP elements has potential biological ramifications in both mutant and wild-type settings. One of the questions raised by our results is whether RIP function is mediated by proteins that specifically recognize these sequences or is mediated by some other property of these elements. The two RIP sequences we identified, RIP317 and RIP1238, are both AT-rich, especially in their core regions. They do not share an obvious consensus sequence, and in fact, their AT-rich character makes it difficult to find meaningful conservation of these elements throughout the genome. Importantly, this AT-rich character raises the possibility that these elements just act thermodynamically to facilitate the DNA unwinding needed to re-initiate DNA replication. Another possibility is that they simply influence nucleosome positioning around origins, as AT-rich DNA tends to be excluded from nucleosomes [53]. These hypotheses, however, are not sufficient to account for RIP function, because we were able to abrogate RIP317 function using mutations that preserved AT content without significantly perturbing their calculated unwinding potential or predicted propensity to exclude nucleosomes [44], [45]. These considerations suggest to us that RIP elements may act through proteins that bind to them. Such a possibility is compatible with the poor nucleosome occupancy over RIP317 that has been observed at its endogenous chromosomal location [35], [54], [55]. A quick attempt to uncover such proteins by screening through yeast transcription factors with potential binding motifs [56], [57] in both RIP317 and RIP1238 did not yield any promising candidates (See Materials and Methods); deletions in NHP6A NHP6B, YAP1, SUM1, YNR063W, GAT4, SMP1, or YOX1 failed to disrupt the function of either RIP. Hence, we are pursuing more systematic studies to identify proteins that bind RIP elements in vivo and are essential for RIP function. If RIPs do indeed work by recruiting proteins near an origin, the distance dependence of RIP function suggests that these proteins may have to interact in close proximity with specific initiation or regulatory protein that assemble at origins. Our work also demonstrates that origins that do not re-initiate in the MC2Ao strain associate with Mcm2-7 by ChIP analysis and thus can at least recruit Mcm2-7 to origins. Apparently, these origins are blocked at an initiation step subsequent to Mcm2-7 recruitment, and the RIP elements confer preferential re-initiation on neighboring origins by deregulating this step. Exactly which step is deregulated by RIP elements is not resolved by our experiments, but there are two major possibilities. The elements could be deregulating the transition between Mcm2-7 recruitment and Mcm2-7 loading, which has been defined in vitro [58] but not yet demonstrated in vivo. Alternatively, they could be deregulating a step following Mcm2-7 loading. We favor the former possibility because the latter requires us to violate a fundamental principle of the current paradigm for re-initiation control [3], [5], namely that this control only targets steps preceding Mcm2-7 loading. Nonetheless, resolution of this question must await the development of more sophisticated in vivo protein-DNA binding assays that are capable of distinguishing recruited from loaded Mcm2-7 at individual origins. Importantly, this role in enabling a step of initiation subsequent to Mcm2-7 origin association distinguishes RIP elements from B2 elements, one of the core elements of budding yeast origins. Both elements are AT rich, positioned 3′ of the T-rich strand of the OBS, and have relaxed positioning requirements relative to the OBS. However, the B2 elements are needed for Mcm association with origins [59], and RIP elements are not. This distinction provides further support for a model in which RIP elements antagonize an inhibitory mechanism, rather than simply promote a normal initiation function. How might RIP317 and RIP1238 locally override a block to Mcm2-7 loading that prevents origins from re-firing in the MC2Ao background? The simplest model is that the block is imposed by one or more of the regulatory mechanisms that remain intact in MC2Ao strains, e. g. CDK phosphorylation of Orc2 and Orc6 [7] or CDK binding to Orc6 [22]. According to this model, RIP elements locally antagonize some or all of these mechanisms, relieving enough of the block to allow detectable re-initiation at RIP-associated origins. This model is consistent with in vitro studies that indicate these inhibitory mechanisms still permit ORC binding and some Mcm2-7 recruitment to origins, but completely block Mcm2-7 loading onto origins [58]. The model is also consistent with our observation that globally antagonizing CDK phosphorylation of ORC in the MC2Ao background by mutating all CDK consensus phosphorylation sites on Orc2 and Orc6 allows many origins to join ARS317 and ARS1238 in re-initiating at detectable levels [12]. However, direct support for this model will require analysis of ORC phosphorylation and CDK binding at origins to determine if they are indeed reduced at RIP-associated origins as might be predicted by the model. We note that the induction of re-initiation in the MC2Ao strain is limited and slow compared to the usual efficiency of origin initiation in a normal S phase. After 3 hr of induction, over one and a half cell cycles for this strain, only 50% and 25% of ARS317 and ARS1238, respectively, have re-initiated. This inefficient re-initiation suggests that RIP317 and RIP1238 only partially antagonize the inhibitory mechanisms blocking Mcm2-7 loading. Such incomplete relief of inhibition may explain why completely antagonizing inhibitory phosphorylation of Orc6 on one CDK consensus site (S116A) can further enhance ARS317 and ARS1238 re-initiation in the MC2Ao strain relative to the MC2A strain (Figure S1). The preferential re-initiation of ARS317 and ARS1238 is reminiscent of the localized re-initiation that occurs in several cases of developmentally programmed gene amplification [60]. One of the best characterized is the amplification of the chorion gene locus in Drosophila ovarian follicle cells during oogenesis. Like the RIP elements identified in this work, an Amplification Control Element (ACE3) of ∼320 bp has been identified that has little origin function on its own and confers preferential re-initiation on a nearby origin (ori-beta). However, the mechanism by which ACE3 and other potential ACE elements promote re-initiation at a select group of origins remains a mystery [60]. Our work in budding yeast offers a conceptual framework for exploring the mechanism of developmentally regulated gene amplification, even if the details prove to be different. For example, characterizing how far the initiation reaction can proceed on the majority of origins that don' t re-initiate may give insight into the key step that allows amplification origins to re-initiate. Similarly, it may be informative to investigate the status of inhibitory modifications on initiation proteins associated with re-initiating origins to see if these modifications are reduced relative to the bulk protein population. In addition to its established role in developmentally programmed gene amplification, there are several hints that DNA re-replication may also contribute to the amplifications and abundant duplications observed in cancer cells. First, we have shown in budding yeast that re-replication arising from deregulated replication initiation proteins can be an extremely efficient source of segmental amplification [2]. Second, overexpression of initiation proteins in murine models has been shown to promote oncogenesis [61]–[63]. Third, overexpression of replication initiation proteins has been observed in some human cancer cells [64]–[67]. And finally, the tandem direct repeat structure of some oncogene amplifications and many of the duplications detected in cancer cells is consistent with the structures that could arise from re-replication [68]. Should re-replication prove to be a new source of copy number variation (and possibly other genomic alterations) in cancer cells, local modulation of origin control, such as that described in this work, could make some regions of the genome more susceptible to re-replication induced genetic alterations than others. One can therefore imagine that an irregular genomic landscape of re-initiation susceptibility could give rise to an irregular genomic landscape of genetic instability in cancer cells. Preliminary indication for such position dependent variability in genetic instability has been obtained by experiments showing that the frequency and structure of DHFR amplification in a cancer cell line was different for different genomic positions of DHFR [69]. Copy number variation may also play an important role in normal cells. For example, gene duplications are thought to provide the functional redundancy that enables the functional diversification of genes during molecular evolution [70]. In addition, copy number increases, which occur with high prevalence in normal human genomes [71], may directly provide phenotypic variation that can be selected for during evolution. In both examples, the mechanism of copy number change is not clear. We speculate that extremely rare re-initiation events may occur despite the presence of normal re-initiation controls and contribute to copy number increases. Should re-initiation drive some of these copy number increases, variable susceptibility of origin re-initiation throughout the genome would be expected to make some regions of the genome more subject to evolutionary change than others. Thus, the presence of a local layer of re-initiation control provided by RIP-like elements may have far reaching ramifications on oncogenesis and evolution.
  Integrative plasmids were used to test RIP-origin re-replication or replication activity in a chromosomal context. These plasmids were all derived from pBJ2889 [2]. This plasmid contains a portable re-replication integration cassette made up of the following elements: Homology Left (sequences centromere proximal to ARS419, which is located at 567 kb on Chromosome IV), the kanMX6 reporter gene [72], the ade3-2p color reporter gene [73], a polylinker, which includes the XbaI restriction site, and Homology Right (sequences centromere distal to ARS419). SpeI – XbaI fragments containing RIP-origin inserts and additional restriction sites were integrated into the XbaI site of the pBJL2889 polylinker, creating a SpeI/XbaI fusion site (TCTAGT) on the ade3-2p side of the insert and re-creating an XbaI (TCTAGA) site on side adjacent to Homology Right. We report the sequence of these clones in Table S1 from the SpeI/XbaI fusion site to the intact XbaI site. The re-replication integration cassette was excised from the plasmid using SacI-NotI or SacI-SalI and introduced into yeast using standard techniques. Integration of these cassettes at ARS419 destroyed its origin activity. The ARS activity of RIP-origin constructs was measured by mitotic stability assays utilizing centromere-containing plasmids. These CEN-ARS plasmids were derived from pFJ11 [36], a plasmid containing ARS317 and CEN4. As a preliminary step, the BamHI site adjacent to CEN4 was destroyed by BamHI digestion, klenow fill-in of the cut overhangs, and blunt-end ligation. The ARS317 in this modified pFJ11 was then replaced with our origin or RIP-origin constructs by cloning these constructs into the HinDIII and EcoR1 sites of the plasmid (exact sequences listed in Table S1). These plasmids were transformed into YJL310 [74] using standard techniques. The full sequence of all insertion and deletion mutants used to alter RIP-OBS spacings are listed in Table S1. They were generated as follows: Genotypes and derivations for all strains used in this manuscript can be found in Table S2. Almost all the MC2Ao yeast strains in this paper were generated from the previously published strain YJL3758 [2] by one or more of the following genetic alterations: (1) integration of a re-replication cassette (described in Plasmids above and detailed in Table S1); (2) deletion of ARS317, ARS418, or ARS1238 (Table S3); (3) deletion of SIR or FKH genes (Table S3) [72], [75], [76]. MC2A strains YJL8923 and YJL8924 are congenic to YJL3758 but have wildtype ORC6 instead of orc6 (S116A). Oligonucleotides used to PCR marked deletion fragments for deleting origins or genes encoding transcription factors are listed in Table S3. Oligonucleotides used in quantitative PCR are listed in Table S4. Synthetic complete medium containing 2% wt/vol dextrose (SDC) was made up as described [77] except that we used twice the concentration of amino acids and purines for all but leucine, which was added to a final concentration of 120 µg/mL, and serine, which was added to a final concentration of 200 µg/mL. Drop out media like SDC-URA, simply lacked the indicated component. For nonselective rich media cells were grown in YEPD (YEP +2% wt/vol dextrose) or YEPRaf (YEP +3% wt/vol raffinose +0. 05% wt/vol dextrose). All cell growth was performed at 30°C. To induce re-replication, freshly thawed log phase cultures in YEPD were extensively diluted into YEPRaff and grown for 12–15 hr until they reached an OD600 of 0. 2–0. 8. At this cell density (approximately 1×107 cells/ml), nocodazole (US Biological N3000) was added to a final concentration of 15 µg/mL for 120–135 min to arrest cells in metaphase. GAL1 promoter driven pGAL-Δntcdc6,2A was then expressed by the addition of 2–3% galactose for 3 hr or 6 hr where indicated. Strains were grown overnight in YEPD at 30°C to an OD600 of 0. 2–0. 4. At this cell density, 50 ng/mL alpha factor was added to arrest cells in G1 phase. Arrested cells were released into fresh YEPD media containing 0. 1 M hydroxyurea (US Biological H9120), 100 µg/mL pronase (EMD 53702), and 15 µg/mL nocodazole (US Biological N3000) to permit a single, slowed S phase to occur. Cultures were harvested after 135 minutes when 30–60% of the genome was replicated as verified by FACS analysis [78]. To increase the sensitivity of detecting initiation activity from the integrated re-replication cassettes, we deleted the closest early origin ARS418 so that its forks would not run through the origins in the cassettes and preclude their initiation. Full details of array CGH data analysis are described in [12]. Briefly: arrays were scanned on a GenePix 4000B scanner and quantified using GenePix 6. 0 (Axon Instruments). The Cy5/Cy3 ratios were normalized such that the average ratio was equivalent to DNA content for that specific point in the cell cycle (e. g. 2C for M arrested or induced samples, and 1. 5C for S phase samples). Medians for these raw normalized data were then calculated across a 10 kb moving window. Smoothed curves were calculated from this moving median using Fourier Convolution Smoothing (FCS). The degree of smoothing is determined by a parameter called the convolution kernel [81], and for the chromosomes we display we used the following values optimized for re-replication profiles: Chromosome III, 9; Chromosome IV, 11. 25; Chromosome V, 9; Chromosome XII, 10. 75. For S phase replication profiles, the convolution kernel for Chromosome IV was set to 6. 25. For presentation purposes, smoothed lines for each individual re-replication or S-phase profile were averaged into one composite profile. Most figures in the manuscript show these composite profiles as black lines surrounded by a gray zone representing ±1 standard deviation. The raw data and the smoothed lines for each individual experiment performed for this work can be seen in Document S1. We note that, because of cross hybridization among the various repetitive sequence elements, these elements (tRNA genes, subtelomeric repeats, Ty elements and long terminal repeats) were removed from the analysis. In the Saccharomyces Genome Database, the two rDNA genes representing the large rDNA repeat arrays are adjacent to a Ty element and additional repeated sequences, so the entire ∼44 kb region between YLR153C and YLR163C was omitted from the analysis. Also, because each chromosome was effectively circularized during the calculation of the moving window median and the FCS, deviations of the smoothed curve from baseline values at one chromosome end can artifactually cause the curve to deviate from baseline at the other end [82]. Thus, when ARS317 preferentially re-initiated at its endogenous location near the right end of Chromosome III, it caused the smoothed re-replication curves to rise at the left end. We have masked the left 20 kb of the smoothed re-replication curves for Chromosome III in Figures 1A, 2B, and S1A, but left the curves unmasked in the individual experimental profiles shown in Document S1. Bar graphs were generated to compare the amount of re-initiation seen in experimental vs control strains. aCGH re-initiation peak heights were measured relative to the expected G2/M copy number (2C) for both experimental and control strains. Replicates of each array were then averaged (xexp and xcont) and a standard deviation calculated (sexp and scont). The ratio xratio formed by xexp divided by xcont was converted to a percentage and plotted as shown. The error for this ratio was calculated by solving the equation: Re-replication of each experimental strain (n = 2) was measured at one of the following re-replicating loci: ChrIII_292 kb (endogenous ARS317), ChrIV_567 kb (transplanted locus), or ChrXII_889 kb (endogenous ARS1238). Relevant control strains lacking (negative control) a re-replicating origin at each location were measured to provide a background (i. e. non re-replicating) baseline. Sample size for these negative control strains ranged from n = 5 to n = 10 as indicated in figure legends. Mean profile heights of the experimental and negative control strains were compared using Welch' s t-test. Significant (p<0. 05) results reject the null hypothesis and confirm that re-replication of sirΔ and fkhΔ strains is significantly different from re-replication of the relevant negative control strain. CEN-ARS plasmids containing RIP-origin, RIP, or origin constructs were transformed into YJL310 [77], a strain with intact re-replication controls. Three independent transformants were inoculated into media selective for the plasmids (SDC-URA) and grown overnight to saturation. Cultures were subsequently diluted back into fresh selective media and grown overnight to an optical density of 0. 1–0. 6. Each log phase culture was plated to five selective (SDC-URA) and five non-selective plates (SDC) at a density of 200–400 cfu/plate. Plates were grown for 3-4 days and the fraction of cells harboring a plasmid was determined by dividing the number of colonies on the selective plates over the number on non-selective plates. Values reported are averaged from the three independent plasmid transformants. ChIP experiments were performed with approximately 20 OD units of cells in a media volume of 50 mL. Cultures were handled as described above for re-replication cultures except induction was restricted to 90 minutes. We reasoned that anti-Mcm ChIP would work best immediately after Mcms were re-loaded onto origin DNA but before most of these origins had re-fired and distributed Mcms throughout the genome. Thus, we selected the 90-minute induction time point as this was the latest induction time before re-replication became visible by array CGH. This rationale is similar to that used in earlier ChIP-chip analysis of re-replicating strains [13]. Terminal cultures were fixed by addition of formaldehyde (37% w/v) to a final concentration of 1%. Fixation proceeded for 15 minutes at room temperature and was quenched by the addition of glycine to a final concentration of 0. 125 M. Fixed cells were harvested by centrifugation, washed once in 1× TE pH 7. 5, and frozen at −80C. Cell pellets were resuspended in 500 µL lysis buffer (50 mM HEPES/KOH pH 7. 5,140 mM NaCl, 1 mM EDTA, 1% Triton, 0. 1% Na-Deoxycholate) with protease inhibitors (Roche mini complete #04693159001+2 mM PMSF) and transferred into 2 mL screw-cap tubes (Sarstedt #72. 694. 006). 0. 5 mm glass beads (Biospec Products 11079–105) were added to the level of the meniscus and cells were disrupted using a FastPrep 24 for two cycles of 45 sec at 6. 0 m/s with 2 min incubation on ice in between. All subsequent steps were performed in low adhesion DNAse/RNAse free 1. 5 mL microfuge tubes at 4°C unless otherwise indicated. Lysates were cleared by centrifugation at 20,000 rcf for 10 min and pellets (containing chromatin) were resuspended in 500 µL of fresh lysis buffer + protease inhibitors. Each pellet was sonicated using a 1/8” tapered microtip attached to a Branson 450 sonicator for 4 cycles of 30 sec at setting 1. 5 with >2 min on ice in-between. The resulting slurry was cleared again by centrifugation at 20,000 rcf for 10 min and the supernatant was retained as whole cell extract (WCE). Immunoprecipitation, washes, and elution were performed on 80–90% of the WCE volume using methods described in [83]. These extracts were exposed to UM174 antibodies (rabbit polyclonal anti-Mcm2-7,1∶500 dilution) [58] (generous gifts from Steve Bell) in the presence of 30 uL slurry of Protein G Dynabeads (Life Technologies, 10004D). Immunoprecipitations were performed for 20 hr at 4°C. Beads were washed 3× with 1 mL of Wash Buffer (10 mM Tris-Cl pH 8,250 mM LiCl, 0. 5% NP-40,0. 5% Na-Deoxycholate, 1 mM EDTA) and 1× with 1 mL of TE (10 mM TrisCl pH 8,1 mM EDTA) with 50 mM NaCl. DNA was eluted from the beads by incubating them in 100 µL of 65°C Elution Buffer (50 mM Tris-Cl pH 8,10 mM EDTA, 1% SDS) for ten minutes. Crosslink reversal and DNA purification was performed essentially as described in [84]. Briefly, IP samples were digested in proteinase K (final concentration 1 mg/mL) for 2 hr at 37°C and incubated at 65°C for 6 hr to reverse crosslinks. WCE samples omitted the proteinase K but were otherwise subjected to the same incubation conditions. DNA from both IP and WCE were purified using PCR purification columns (Qiagen Inc 28106) and eluted into 300 µL of 1× TE pH 8. For each genotype, three independent cultures were analyzed and the average fold enrichments of origin DNA by ChIP were reported. The IP and WCE DNA samples from each individual culture were analyzed in triplicate on a Stratagene MX3000P qPCR machine using primer pairs listed in Table S4. Each reaction was performed using Power SYBR Green PCR Master Mix (Applied Biosystems) in a total volume of 20 µL with primers at a final concentration of 300 nM. Because of the AT-rich nature of template origin DNA, we used an annealing temperature of 57°C and an extension temperature of 65°C. Fold enrichment of the assayed DNA segments over the average of two non-origin DNA segments (ADH1 and SLH1) was calculated using the 2-ΔΔCt method essentially as described [85]. The UNIPROBE database of in vitro DNA binding specificities [57] was searched using RIP317 and RIP1238 sequences. The search was restricted to S. cerevisiae datasets and the stringency filter was set to the lowest setting. Nonessential candidate RIP-binding proteins found in both sequences were NHP6A NHP6B, YAP1, SUM1, YNR063W, GAT4, SMP1, and YOX1. These factors were knocked out genetically and the resulting strains were tested for re-replication activity at ARS317 and ARS1238. All array CGH data from this study have been deposited in the Gene Expression Omnibus (GEO) (http: //www. ncbi. nlm. nih. gov/geo) database (Series Accession #GSE55420).
summary: Eukaryotic organisms have hundreds to thousands of DNA replication origins distributed throughout their genomes. Faithful duplication of these genomes requires a multitude of global controls that ensure that every replication origin initiates at most once per cell cycle. Disruptions in these controls can result in re-initiation of origins and localized re-replication of the surrounding genome. Such re-replicated genomic segments are converted to stable chromosomal alterations with extraordinarily efficiency and could provide a potential source of genomic alterations associated with cancer cells. This publication establishes the existence of a local layer of replication control by identifying new genetic elements, termed re-initiation promoters (RIPs) that can locally override some of the global mechanisms preventing re-initiation. Origins adjacent to RIP elements are not as tightly controlled and thus more susceptible to re-initiation, especially when these global controls are compromised. We speculate that RIP elements contribute to genomic variability in origin control and make some regions of the genome more susceptible to re-replication induced genomic instability.
keywords: biochemistry
  dna replication
  nucleic acids
  cell biology
  biology and life sciences
  dna
  molecular cell biology
title: Regulatory Mechanisms That Prevent Re-initiation of DNA Replication Can Be Locally Modulated at Origins by Nearby Sequence Elements