Detecting danger is one of the foremost tasks for a neural system. Larval parasitoids constitute clear danger to Drosophila, as up to 80% of fly larvae become parasitized in nature. We show that Drosophila melanogaster larvae and adults avoid sites smelling of the main parasitoid enemies, Leptopilina wasps. This avoidance is mediated via a highly specific olfactory sensory neuron (OSN) type. While the larval OSN expresses the olfactory receptor Or49a and is tuned to the Leptopilina odor iridomyrmecin, the adult expresses both Or49a and Or85f and in addition detects the wasp odors actinidine and nepetalactol. The information is transferred via projection neurons to a specific part of the lateral horn known to be involved in mediating avoidance. Drosophila has thus developed a dedicated circuit to detect a life-threatening enemy based on the smell of its semiochemicals. Such an enemy-detecting olfactory circuit has earlier only been characterized in mice and nematodes.
The olfactory system is tuned to detect cues important to survival and reproduction. One extremely important function is to detect danger [1]. For humans, the odor of smoke is a good example of such an important olfactory warning signal to which we are highly sensitive. Only in two cases, however, have olfactory circuits specifically detecting predator or pathogen odor been characterized regarding the involved olfactory receptor and the danger-derived ligand; the cat urine detection in mice [2] and the pathogen detection in the nematode Caenorhabditis elegans [3]. For most insects, olfaction is the primary sense. They use it to find and judge food and oviposition sites [4,5], mates [6,7], or competitors [8], but so far no circuitry has been shown to be involved in detecting life-threatening enemies. A major cause of death in larvae of the vinegar fly D. melanogaster is to be injected with eggs from a parasitoid wasp. The eggs develop into parasitoid larvae, which consume the fly larva from the inside. In some wild subpopulations, up to 80% of the fly larvae are parasitized by different parasitoid wasp species, with Leptopilina boulardi and L. heterotoma being the most common ones [9,10]. There is thus a very good reason for fly larvae to avoid being parasitized and for female flies to avoid laying eggs where parasitoids are present. With this background, we investigated the reaction of larval and adult vinegar flies to the smell of parasitoids. Both larvae and ovipositing flies showed a clear avoidance behavior to otherwise attractive food and oviposition substrates after these had been perfumed with a Leptopilina parasitoid bouquet. We could also demonstrate that in adult flies, avoidance was mediated by the ab10B neuron coexpressing the so-far orphan receptors Or49a and Or85f. This in turn allowed us to identify (-) -iridomyrmecin—a defensive allomone and sex pheromone component of Leptopilina [11,12]—as the sole ligand for Or49a and two other parasitoid odorants ( (R) -actinidine and several stereoisomers of nepetalactol) as ligands for Or85f. As the corresponding neuron in fly larvae only expresses Or49a but not Or85f, larval detection of parasitoids was found to be governed only by iridomyrmecin. When we activated or inactivated the neurons artificially, we could show that they are necessary and sufficient to govern parasitoid avoidance behavior. Recent investigations revealed that the fly has also developed several other survival strategies to escape parasitoid pressure. Female flies prefer ethanol-rich oviposition sites after they have visually recognized parasitoids. As D. melanogaster larvae have higher ethanol tolerance than their parasitoids, the flies self-medicate their offspring [13]. Furthermore, seeing parasitoids leads to sharp decline in flies’ oviposition [14]. Finally, upon wasp attack, fly larvae respond with a specific rolling behavior that occasionally flips the attacker to the back [15] and is mediated by a multimodal circuit that includes mechanosensory as well as nociceptive pathways [16]. All these evolutionary adaptations show how important it is for the fly to escape its deadly enemies, the parasitoids.
We started our experiments with the main targets of the parasitoid and tested whether Drosophila larvae are repelled by the odor of parasitoid wasps (L. boulardi). Larvae were strongly repelled by the body wash of parasitoids (Fig 1A, for the behavioral data all figures are based on, see S1 Data). Orco mutant larvae, however, lacking functional odorant receptors (ORs), were not repelled (Fig 1A, grey shaded area), indicating that the avoidance behavior is elicited by volatile cues detected by ORs. We next examined the behavior in adult flies. In T-maze and trap assays, we did not observe any avoidance of parasitoid odor (Fig 1B). However, female flies strongly avoided the body wash odor when choosing an oviposition site (Fig 1B). Again, Orco mutant females lacked this avoidance (Fig 1B, grey shaded area). We conclude that the smell of L. boulardi is repellent to D. melanogaster larvae and ovipositing flies, and that this repellency is mediated by ORs. Obviously, the decision to oviposit seems to be governed by a circuit that is not directly involved in mediating attraction in adult flies—a finding that is in accordance with previous studies showing that both acetic acid [17] and limonene [18] are mediating oviposition but do not attract flies. As the parasitoids do not attack adult flies but only larvae, a general avoidance behavior of adult flies—apart from oviposition avoidance—does not seem to be adaptive. We next identified the olfactory sensory neuron (s) (OSN) involved in detecting the parasitoid smell. Drosophila larvae express 23 different ORs, of which 13 are also expressed in the adult fly [19]. The identification of individual OSN responses in larvae is almost impossible, as all OSNs are colocalized in a single morphological structure, the dorsal organ. However, recording from identified adult OSNs is possible [20–24]. We therefore used a set of diagnostic odors (S1 Fig) to identify adult OSN types and afterwards performed combined gas chromatography-single sensillum recording (GC-SSR) experiments with the headspace of L. boulardi. We tested all 48 physiologically different OSN types present on the fly antennae and palps. Although the amount of active odor within the headspace was too low to be visible in the GC trace, a repeatable and strong response was elicited from the ab10B OSN at a specific GC retention time. No other OSN type responded to the extract (Fig 2A). To identify the active compound, we collected a larger quantity of odors by washing wasps in dichloromethane. When repeating the GC-SSR experiments with this wash, the ab10B OSN became activated at the same retention time as found with the headspace (Fig 2B), but now with a visible GC peak. In addition, the same OSN responded to two additional compounds. Using mass spectrometry, we identified the three active peaks as a non-identified isomer of nepetalactol, (R) -actinidine, and (-) -iridomyrmecin (S2 Fig) and confirmed the identification by repeating GC-SSR experiments using synthetic standards (Fig 2B). Furthermore, dose-response experiments revealed that the ab10B neuron exhibited the highest sensitivity to iridomyrmecin (Fig 2C). Nepetalactol is one of the major compounds of the volatile oil of catnip [25] and has been shown to be an insect repellent [26]. As an insect allomone, however, so far it has only been described for aphids [27]. Both actinidine and iridomyrmecin have been shown to be released by parasitoids. Actinidine acts as a defense compound [28], while iridomyrmecin, which was first described as a defensive compound of the Argentine ant, Linepithema humilis [29] fulfills many functions in the parasitoid wasps of the genus Leptopilina [11]. They use iridomyrmecin to defend themselves against predators, to avoid competition among females, and as a major component of the female sex pheromone [11,12]. As a consequence of its ubiquitary use in the chemical communication of Leptopilina, the wasps constantly release at least small amounts of iridomyrmecin, which makes it an ideal key substance to reveal the presence of these parasitoids to their host Drosophila. As the ab10B OSN responds to these iridoids, and as it is one of the cases where two ORs (Or49a and Or85f) are coexpressed within the same OSN type in the adult fly [23], we next explored the role of the individual receptors in parasitoid odor detection. We selectively expressed either Or49a or Or85f ectopically in a Drosophila mutant ab3A neuron (i. e. , a neuron lacking its own receptor Or22a [30]) and again performed GC-SSR experiments with the body wash of L. boulardi. OSNs expressing Or49a responded to iridomyrmecin exclusively (Fig 2D). Of the 16 possible stereoisomers of iridomyrmecin, only 3 (with (-) -iridomyrmecin being the most abundant) have been described to occur in the genus Leptopilina [11,12]. When testing those, we found that OSNs expressing Or49a responded only to (-) -iridomyrmecin, but not to (+) -iridomyrmecin or (+) - or (-) -isoiridomyrmecin (S5 Fig). When we tested OSNs misexpressing Or85f, they became activated by (R) -actinidine and nepetalactol (Fig 2D). Further investigation revealed that the ab10B neuron detected both enantiomers of actinidine (although only (R) -actinidine is present in Leptopilina wasps). Because of chromatographic limitations, we were unable to determine the absolute configuration of the nepetalactol stereoisomer produced by the wasps. However, all synthetically available stereoisomers (i. e. , 1S4aR7R7aS-nepetalactol, 1R4aS7S7aS-nepetalactol and their enantiomers) activated OSNs expressing Or85f (S6 Fig). Thus, both ORs expressed in the ab10B OSN are involved in the detection of the parasitoid volatile blend although sensitive to different components thereof. To further test the specificity of the ab10B OSN, we screened neurons ectopically expressing one of both receptors with a set of 232 compounds including odorants from a wide range of different chemical classes (Fig 2E, S4 Fig). From these results and from another study, where Or49a was tested against almost 500 odorants [31], we conclude that the ab10B neuron is highly specific to the Leptopilina odorants (-) -iridomyrmecin (Or49a), and (R) -actinidine and nepetalactol (Or85f). The spectrum of detection by the ab10B OSN has thus been widened, not by lessening the specificity of a single receptor but by adding a second highly specific one. We next measured odor-induced activity patterns in the D. melanogaster adult antennal lobe (AL) (Fig 2F). We used the Gal4-UAS system to express the Ca2+-sensitive reporter GCaMP3. 0 [32] under control of the Or49a promoter. Some of the former studies [23,33–36] did not observe expression of Or49a. However, we expressed GCaMP3. 0 under control of Or49a-Gal4 and validated the functionality of our Or49a-Gal4 on the adult antenna using confocal microscopy (S7 Fig). All three parasitoid compounds elicited a strong activation. When we used the GH146 enhancer trap line, all three compounds elicited a clear and exclusive activation at the output level of the DL4 glomerulus. Hence, the calcium imaging results confirmed the conclusion from the SSR experiments that the ab10B neurons expressing Or49a and Or85f become activated by the parasitoid odorants (R) -actinidine, several enantiomers of nepetalactol, and (-) -iridomyrmecin. They also showed that these signals transfer via dedicated projection neurons to (PNs) higher brain centers. Recent findings suggest that laterally situated glomeruli of the AL mainly become activated by aversive stimuli [37]. Our finding that the DL4 glomerulus becomes activated by odorants governing parasitoid avoidance further supports this segregated representation of positive and negative innate olfactory valence within the medial and lateral AL, respectively. Interestingly, in contrast to adult flies, larvae do not express Or85f but only Or49a [19]. As expected, when performing GC-coupled extracellular recordings from the dorsal organ with the body wash of parasitoids, we did not observe any responses to the Or85f ligands actinidine and nepetalactol, but observed a strong response to the Or49a ligand iridomyrmecin (Fig 2G). Contrary to adult females, larvae do not only lose part of their offspring but become killed by the parasitoid. Hence, the larval olfactory system should even be better suited to detect parasitoid odorants. It therefore remains unclear why Drosophila larvae do not express Or85f, and by that are restricted to detect iridomyrmecin only. We next investigated how the information transfers to the next levels of olfactory processing, the mushroom body (MB) calyx and the lateral horn (LH). When characterizing the innervation patterns of DL4 PNs we found that the projection pattern of these neurons in higher brain centers was stereotypic among individual flies as shown for other PNs in previous studies [38,39]. We next compared how this innervation pattern relates to that of DA2-PNs conveying information from another highly repellent olfactory pathway mediating odor information regarding the presence of detrimental microbes [40]. Interestingly, the comparison revealed that the projection pattern of both PN types overlapped strongly within a posteriomedial domain in the LH and in the base MB calyx (Fig 3). The overlapping pattern of two PN populations that both convey information on odorants of strongly negative hedonic valence is in accordance with recent findings that olfactory stimuli with negative and positive valence activate separate domains in the LH [41–43]. We next tested whether the presence of one of the ab10B ligands was sufficient to inhibit oviposition. Flies exhibited a strong tendency to avoid the Or85f ligand (R) -actinidine (we applied 1 μg, i. e. , ca 50 wasp equivalents) and oviposited significantly less when confronted with 3 wasp equivalents (i. e. , 1 μg) of the Or49A ligand (-) -iridomyrmecin (S8 Fig). Furthermore, larvae only expressing Or49a consequently became significantly repelled by the Or49a ligand (-) -iridomyrmecin but did not avoid the Or85f ligand (R) -actinidine (S8 Fig). Do ovipositing flies lacking a functional ab10B OSN still avoid the parasitoid smell? To silence the ab10B neurons specifically, we expressed the temperature-sensitive mutant dynamin Shibirets [44] under the control of the Or49a promoter. At the restrictive temperature (30°C), flies carrying this construct did not display any oviposition avoidance toward the parasitoid wash (Fig 4A), while they showed a strong aversion to the wash at the permissive temperature (23°C). Parental lines and wildtype flies avoided the parasitoid odor at the restrictive temperature. As Or49a and Or85f are coexpressed in the ab10B neuron, expressing Shibirets under the control of the Or49a promotor should erase the functional significance of both receptors. However, we also expressed Shibirets under the control of the Or85f receptor, which again yielded in reduced olfactory avoidance, when flies were tested at the restrictive temperature (Fig 4A). Larvae that expressed Shibirets under the Or49a promoter again exhibited no avoidance at the restrictive temperature, while the permissive temperature resulted in avoidance not different from wild type and parental lines (Fig 4B). As the larvae at the restrictive temperature still targeted the attractant ethyl butyrate, the loss of behavior at restrictive temperature seems to be ligand- and Or49a-specific. We thus conclude that activation of OSNs expressing Or49a in larvae and Or49a and Or85f in adult flies is necessary for behavioral avoidance of parasitoid odors. To test whether the activation of ab10B neurons was sufficient for avoidance of parasitoid smell, we expressed the photo-activated cation-selective channel channelrhodopsin-2 (ChR2) in the neurons by using either the Or49a-Gal4 or the Or85f-Gal4 driver. We then tested how blue light (470 nm, i. e. , the wave length activating the expressed cation channel) affected adult oviposition and larval crawling behavior. Contrary to wild-type flies and parental lines, CHR2-females avoided illuminated oviposition sources (Fig 4C). The same held true for CHR2-larvae that became significantly more repelled by blue light than control lines (Fig 4D). We conclude that activation of the ab10B neuron and its larval equivalent is necessary and sufficient to elicit avoidance of parasitoid smell in female D. melanogaster adults and larvae, respectively. D. melanogaster becomes parasitized not only by Leptopilina wasps but also by other wasp genera. We asked whether the ab10B neuron also responded to odorants from predators or other parasitoid species (Fig 5A). Therefore, we performed GC-SSR experiments with ab10B neurons using body washes of four potential Drosophila predators and of four additional parasitoid wasp species (L. heterotoma, Asobara tabida, A. japonica, and Trichopria spec.). While the neurons again responded to iridomyrmecin, actinidine, and nepetalactol present in L. heterotoma, we could not identify any further ab10B ligand—neither in the odor of the insect predators nor in that of the other parasitoid species. Accordingly, D. melanogaster avoided the odor of L. boulardi and L. heterotoma but neither ovipositing adult flies nor larvae displayed any odor-based avoidance behavior (Fig 5B) towards the predators or the parasitoids belonging to other genera. We conclude that the ab10B-dependent avoidance behavior is restricted to one of the most harmful parasitoid genera [9,10,45], the iridoid-producing Leptopilina. Finally, we asked whether odor-based detection and avoidance of Leptopilina wasps is specific for D. melanogaster. Parasitoids of the genus Leptopilina do not only target D. melanogaster but also many other Drosophila species [46,47]. All of the 12 Drosophila species, whose genomes have been published, express Or49a and Or85f. However, the orthologs of Or49a (Or85f) only show 57. 1% (63. 5%) amino acid similarity with a dN/dS value of 0. 23 (0. 19), respectively. We therefore asked whether detection and avoidance can also be observed in D. simulans, D. mauritania, D. virilis, and D. suzukii. As all four species either originate from Africa or Asia, i. e. , continents where wasps of the genus Leptopilina also occur, they should all have a long evolutionary history with at least one Leptopilina species. In GC-SSR experiments with each of the Drosophila species, we always found an OSN that responded to the headspace of L. boulardi (Fig 5C). We cannot exclude that the OSN responding to wasp odors in those flies expressed another so-far unidentified receptor. However, in all species, the colocalized neurons exhibited similar responses like the D. melanogaster ab10A neuron (S9 Fig, for raw data see S1 Data). Therefore—also in the other species—it was most probably the ab10 sensillum that detected the wasp odor, which supports the general involvement of Or49a and Or85f in parasitoid detection of the genus Drosophila. Furthermore, larvae and ovipositing adults of all tested species avoided the parasitoid headspace (Fig 5D and 5E). We conclude that iridoid-based Leptopilina avoidance is a conserved feature of several Drosophila species. Whether or not the detection of the Leptopilina odors is mediated in all species by Or49a and Or85f remains open. However, the comparatively low conservation of these receptors is not necessarily a counterargument. It has been shown before that receptors exhibiting low sequence conservation in different Drosophila species still can be tuned to the same ligands [48]. Another functionally conserved receptor, Or56a, on the other hand is also one of the molecularly most conserved receptors among the drosophilids. Evolution obviously finds different ways to preserve function among olfactory receptors. We show that drosophilid flies and their larvae avoid the odor of one genus of parasitoids. The question, why Drosophila exhibits a dedicated circuit to the odors of Leptopilina wasps while the odors of the other tested parasitoid wasps did not evoke any olfactory avoidance behavior at all, remains open. Although Leptopilina seems in some habitats to be the most abundant parasitoid, parasitation rates by other genera can become high [9,10,45]. However, Drosophila does not only exhibit olfactory parasitoid avoidance but also avoidance based on visual cues [13,14] as well as on mechanosensory and nociceptive cues [15]. It, therefore, might be that the nonolfactory cues become especially important in the avoidance of those parasitoids that do not carry any of the ab10B ligands. The animals tested in our experiments had never experienced parasitoids before. Hence, the observed avoidance behavior is governed by innate rather than learned negative hedonic valence. Dedicated pathways involved in detection and processing of innate valence are usually restricted to odorants of outstanding ecological valence [49] and are not restricted to insects [50]. Zebrafish detect the death-associated odor cadaverine based on the specific and highly sensitive receptor TAAR13c [51]. Mice detect predators like cats, snakes, and rats [52] as well as conspecifics [53] based on olfactory cues. It has been shown that the trace amine-associated receptor 4 (TAAR4) mediates innate repellence to cat urine [2], while TAAR5 detects a compound in mouse urine and mediates attraction towards conspecifics [53]. Recent findings in Drosophila revealed several dedicated olfactory pathways that either mediate avoidance [40,54], mating [55], or oviposition [18]. Enemy avoidance based on learned cues have been shown for C. elegans. After first contact with the pathogen Serratia marcescens, the nematode learns to avoid the bacterium based on a specific bacterium-derived peptide [3]. We show that the fly’s ab10B neuron specifically mediates innate aversion towards the parasitoid-derived odorants (-) -iridomyrmecin, (R) -actinidine and different enantiomers of nepetalactol. To our knowledge, this presents the first case in which an animal not only smells and avoids its enemy but does this based on the enemy’s semiochemicals, including a sex pheromone. The evolutionary strategy to use the odor-based sex communication system of an enemy to avoid it should be highly adaptive. The possibility for evolutionary countermeasures from the parasitoid side should be limited as it is difficult to resign the sex communication system.
Adult flies were immobilized in pipette tips, and the third antennal segment or the palps were placed in a stable position onto a glass coverslip. Sensilla were localized under a binocular at 1,000x magnification and the extracellular signals originating from the OSNs were measured by inserting a tungsten wire electrode in the base of a sensillum. The reference electrode was inserted into the eye. Signals were amplified (10x; Syntech Universal AC/DC Probe, www. syntech. nl), sampled (10,667 samples/s), and filtered (100–3,000 Hz with 50–60 Hz suppression) via USB-IDAC connection to a computer (Syntech). Action potentials were extracted using Syntech Auto Spike 32 software. We used a set of diagnostic odors (S1 Fig, i. e. , odors that have been shown to activate neurons expressing a specific receptor [20]) to identify adult OSN types. As in D. melanogaster, the colocalization of neurons expressing different receptors in a single sensillum is conserved; we were able to identify even those neurons with orphan receptors by checking out the response patterns of colocalized neurons. Neuron activities were recorded for 10 s, starting 2 s before a stimulation period of 0. 5 s. Responses from individual neurons were calculated as the increase (or decrease) in the action potential frequency (spikes/s) relative to the prestimulus frequency. For GC stimulation, 1 μl of the odor sample (1 μl of actinidine and nepetalactol solutions correspond to ca 50 wasp equivalents, 1 μl of iridomyrmecin solution corresponds to 3 wasp equivalents, 1 μl of wasp wash corresponds to 0. 1 wasp equivalent) was injected onto a DB5 column (Agilent Technologies, http: //www. agilent. com), fitted in an Agilent 6890 GC, equipped with a four-arm effluent splitter (Gerstel, www. gerstel. com), and operated as previously described (Stökl et al. , 2010) except for the temperature increase, which was set at 15°C min-1. GC-separated components were introduced into a humidified airstream (200 ml min-1) directed toward a mounted fly. Signals from OSNs and FID were recorded simultaneously. Solvent extract of 100 wasps were analysed by gas chromatography coupled with mass spectrometry (GC-MS). Samples were analysed on a Shimadzu GC2010 gas-chromatograph (GC) connected to a QP2010 plus mass-spectrometer (MS; Shimadzu, Germany) and an Agilent 7890GC coupled with an 5975c MS (Agilent Technologies, Germany). The Shimadzu GC-MS was equipped with either a nonpolar capillary column (BPX-5,30 m length, 0. 25 mm inner diameter, 0. 25 μm film thickness; SGE Analytical Science, UK), or a BetaDex 225 cyclodextrin column (30 m length, 0. 25 mm inner diameter, 0. 25 μm film thickness; Sigma-Aldrich, Germany) or a GammDex 120 cyclodextrin column (30 m length, 0. 25 mm inner diameter, 0. 25 μm film thickness; Sigma-Aldrich, Germany). When using the BPX5 column, Helium was used as a carrier gas with a constant linear velocity of 50 cm/s-1 and the temperature program of the GC oven started at 80°C and was raised by 5°C/min-1 to 280°C. For both cyclodextrin columns, the carrier gas flow was reduced the 35 cm/s-1 and the oven temperature started at 50°C and was raised by 2°C/min-1 to 220°C. The MS was run in electron impact (EI) mode at 70 eV and set to a scan range from 35 to 600 mz-1. All samples were injected splitless. The Agilent GC-MS was equipped with a Cyclosil-B cyclodextrin column (30 m length, 0. 25 mm inner diameter, 0. 25 μm film thickness; Agilent Technologies, Germany). Helium was used as a carrier gas with a constant linear velocity of 36 cm/s-1. The initial temperature of the GC oven of 40°C was held for 2 min and afterwards raised by 2°C/min-1 to 170°C and then with 70°C to 250°C. The MS was run in electron impact (EI) mode at 70 eV and set to a scan range from 33 to 350 mz-1. All samples were injected splitless. Compounds were identified by comparing the mass spectrum and retention time with that of synthetic reference compounds (S2 Fig). Stereoisomers of Iridomyrmecin were synthesized as described in [12] and [57]. Actinidine and Nepetalactol were synthesized as described by [58]. Flies were prepared for optical imaging as previously described by [59]. Briefly, flies were anesthetized on ice; the head capsule was opened by incising the cuticle between the antennae and the eyes. With the brain immersed in Ringer’s saline (130 mM NaCl, 5 mM KCl, 2 mM MgCl2,2 mM CaCl2,36 mM sucrose, 5 mM Hepes, [pH 7. 3]), the ALs were exposed by removing muscle tissue, glands, and the trachea. We used a Till Photonics imaging system with an upright Olympus microscope (BX51WI) equipped with a 20x Olympus objective (XLUM Plan FL 20x/0. 95W). A Polychrome V provided light excitation (475 nm) and a filter set ensured passage of only relevant wavelengths (excitation: SP500, dicroic: DCLP490, emission: LP515). The emitted light was captured by a CCD camera (Sensicam QE, PCO AG) with a symmetrical binning of 2 (0. 625 x 0. 625 μm/pixel). For each measurement, a series of 40 frames was taken (4 Hz). Odors were applied for 2 sec. Pure compounds were diluted in mineral oil (Carl Roth GmbH + Co. KG); 6 μl of the diluted odors were pipetted onto a small piece of filter paper (~1 cm2, Whatman), placed inside a glass Pasteur pipette. Filter papers were prepared ca. 30 min before every experimental session. For odor application, a stimulus controller (CS-55, Syntech) provided a continuous air flow (l/min) in which odor injection was applied via two disposable Pasteur pipettes. For odor stimulation, the air stream switched from a blind Pasteur pipette to the stimulus pipette. Calcium imaging recordings were processed using custom-written software in IDL (ITT Visual Information Solutions) as described in detail in [59]. An in vivo 3-D atlas of the Drosophila AL [60] served to link the calcium signals to identified glomeruli. Neurons were intracellulary stained with biocytin or Lucifer yellow and histologically processed as described previously [40,61]. For brain neuropil background staining, the synaptic antibody nc82 [62] was used. High-resolution scans were done with a Zeiss LSM 510 confocal microscope using a 63 x water immersion objective. Image stacks were imported to Amira 5. 6, and neuronal arborizations and neuropil were manually segmented. For neuron reconstruction, the Skeleton plugin [63] of Amira was used. For registration of datasets, a label template of the central brain (MB and LH) was chosen out of n = 58 preparations following the method of [64]. The warping of segmented labels of the MB and LH onto the template was done in a two-step process: an affine transformation with 12 degree of freedom (12 DOF) followed by an elastic registration using modules of Amira. The calculated transformation matrix was applied to the neuron reconstruction and thus transformed to the template reference space.
Detecting danger is a fundamental task for an animal. Larval parasitoids constitute clear danger to the vinegar fly D. melanogaster as up to 80% of fly larvae become parasitized in nature. We show that Drosophila larvae crawl away from places that smell like the main parasitoid enemies, Leptopilina wasps. Furthermore, Drosophila adult females avoid laying eggs at those places. This avoidance is mediated via a highly specific olfactory sensory neuron type that is tuned to detect three odors of the parasitoid, including the wasps sex pheromone iridomyrmecin. We identify the neuron type, the receptors, and the odor ligands that mediate this behavior and also show that this neuronal system is both necessary and sufficient to govern the parasitoid avoidance behavior. We also find evidence that this odor-based Leptopilina wasp avoidance is conserved across several Drosophila species.
Drosophila Avoids Parasitoids by Sensing Their Semiochemicals via a Dedicated Olfactory Circuit