Neurons of the statoacoustic ganglion (SAG) transmit auditory and vestibular information from the inner ear to the hindbrain. SAG neuroblasts originate in the floor of the otic vesicle. New neuroblasts soon delaminate and migrate towards the hindbrain while continuing to proliferate, a phase known as transit amplification. SAG cells eventually come to rest between the ear and hindbrain before terminally differentiating. Regulation of these events is only partially understood. Fgf initiates neuroblast specification within the ear. Subsequently, Fgf secreted by mature SAG neurons exceeds a maximum threshold, serving to terminate specification and delay maturation of transit-amplifying cells. Notch signaling also limits SAG development, but how it is coordinated with Fgf is unknown. Here we show that transcription factor Tfap2a coordinates multiple signaling pathways to promote neurogenesis in the zebrafish inner ear. In both zebrafish and chick, Tfap2a is expressed in a ventrolateral domain of the otic vesicle that includes neurogenic precursors. Functional studies were conducted in zebrafish. Loss of Tfap2a elevated Fgf and Notch signaling, thereby inhibiting SAG specification and slowing maturation of transit-amplifying cells. Conversely, overexpression of Tfap2a inhibited Fgf and Notch signaling, leading to excess and accelerated SAG production. However, most SAG neurons produced by Tfap2a overexpression died soon after maturation. Directly blocking either Fgf or Notch caused less dramatic acceleration of SAG development without neuronal death, whereas blocking both pathways mimicked all observed effects of Tfap2a overexpression, including apoptosis of mature neurons. Analysis of genetic mosaics showed that Tfap2a acts non-autonomously to inhibit Fgf. This led to the discovery that Tfap2a activates expression of Bmp7a, which in turn inhibits both Fgf and Notch signaling. Blocking Bmp signaling reversed the effects of overexpressing Tfap2a. Together, these data support a model in which Tfap2a, acting through Bmp7a, modulates Fgf and Notch signaling to control the duration, amount and speed of SAG neural development.
Vestibular and auditory information is transmitted from the inner ear to the hindbrain via neurons of VIII. th cranial ganglion, also known as the stato-acoustic ganglion (SAG). SAG neurons are formed by a complex but poorly understood multi-step process that begins in the otic vesicle, the precursor of the inner ear. In the first step, SAG neuroblasts are specified in the floor of the otic vesicle and are marked by the expression of proneural gene neurogenin1 (ngn1) [1,2]. After specification, neuroblasts delaminate from the otic epithelium via epithelial-mesenchymal transition and migrate to a region between the otic vesicle and hindbrain. In zebrafish, markers of later stages of differentiation are usually not expressed within the otic epithelium. Upon delamination, however, neuroblasts quickly lose expression of ngn1 and upregulate the related factor neurod [1,3]. neurod-expressing cells form a population of migrating and proliferating precursors called the transit-amplifying (TA) pool [4]. As cells in the TA pool differentiate into mature neurons they lose expression of neurod and upregulate early neuronal markers islet1 and islet2b [5]. Newly formed neurons extend processes bi-directionally to connect sensory epithelia with central targets in the hindbrain. SAG development in chick and mouse embryos follows a similar course except that transit-amplification and expression of NeuroD and Isl1/2 begin while neuroblasts still reside within the otic epithelium [2,6, 7]. We previously showed that Fgf signaling regulates each step in SAG development in zebrafish [5]. Specification of SAG neuroblasts is initiated by a low level of Fgf signaling. As SAG neurons mature they begin to express fgf5 such that rising levels of Fgf eventually become inhibitory to ngn1 expression in the otic vesicle. Consequently, neuroblast specification starts to decline after 24 hpf and ceases entirely by 42 hpf [5,8]. Elevated Fgf also delays terminal differentiation of cells in the TA pool. The TA pool is thereby maintained as a relatively stable population in which the rate of proliferation closely matches the rate of terminal differentiation. The otic vesicle originates from an ectodermal thickening called the otic placode. The otic placode, along with all other cranial placodes, emerges from a contiguous region of pre-placodal ectoderm (PPE) that forms around the anterior neural plate by the end of gastrulation [9,10]. In zebrafish competence to form PPE is regulated by four transcription factors: Tfap2a, Tfap2c, Gata3 and Foxi1 [9,11]. These transcription factors are also essential for later development of a subset of cranial placodes, including the otic placode. For example, in response to inductive Fgf signaling foxi1 expression upregulates in nascent otic/epibranchial placodes; and disruption of foxi1 leads to severe deficiencies of epibranchial and otic tissue in zebrafish [12–14]. A similar role has been shown recently for Foxi3 in mouse and chick [15,16]. Expression of gata3 also regulates otic development, becoming localized to the nascent otic placode [17] and to discrete regions of the otic vesicle [18–20]. Disruption of Gata3 in mouse causes severe defects in otic vesicle development [18], including deficiencies of sensory epithelia and improper wiring of auditory neurons [21–23]. In contrast, less is known about later roles of tfap2a/c. tfap2a is best known for its role in the early differentiation and survival of neural crest cells [24–32] and together with tfap2c is indispensible for neural crest specification in zebrafish [26,28]. However, whether tfap2a/c genes regulate later development in the otic placode and vesicle has not been investigated. Here we report that tfap2a is expressed throughout the nascent otic placode and is later restricted to a ventrolateral region in the otic vesicle overlapping with the neurogenic domain. Misexpression of tfap2a leads to excess specification and precocious differentiation of SAG neurons whereas knockdown of tfap2a causes reduced neurogenesis and delayed differentiation of SAG precursors. Further investigation revealed that tfap2a acts non-autonomously through upregulation of bmp7a, which in turn restricts Fgf and Notch signaling to promote specification and differentiation of SAG precursors.
To begin to assess potential functions of tfap2a in otic development, we examined expression of tfap2a in the otic placode and early otic vesicle in zebrafish. At 14 hpf (10 somites) when otic cells first form a morphological placode, tfap2a is expressed broadly throughout the placode as shown by co-staining for the early otic marker pax2a (Fig. 1A-B). The level of tfap2a expression varies, with higher levels in dorsal and lateral otic cells. Otic expression in general appears much weaker than in surrounding neural crest cells. By 24 hpf, expression of tfap2a in the otic vesicle is restricted to ventrolateral cells. This domain partially overlaps with the neurogenic domain of the otic vesicle, marked by the proneural gene ngn1 (Fig. 1C-D). Neurogenesis declines sharply by 30 hpf and ceases entirely by 42 hpf [5,8]. Similarly, the level of tfap2a gradually declines in the neurogenic domain after 30 hpf and is no longer detectable by 48 hpf (Fig. 1E-I). Despite initial expression of tfap2a in at least some neuroblasts in the otic vesicle, expression is lost in most neural precursors as they delaminate from the otic vesicle. Mature neurons of the statoacoustic ganglion (SAG), marked by expression of Isl1, show no detectable expression of tfap2a (Fig. 1J). These data are consistent with the possibility that tfap2a is involved in at least some aspects of neurogenesis in the otic vesicle. Expression patterns of tfap2a, ngn1, and other key genes involved in SAG development are summarized in Fig. 1K. To examine the possibility that expression is conserved in amniote vertebrates, we examined expression of Tfap2a in chick embryos. In agreement with the patterns observed in zebrafish, chick embryos also show expression in ventrolateral regions of the otic vesicle (Fig. 1L-Q). Moreover, in chick as in zebrafish, the domain of Tfap2a expression abuts the sensory domain with little or no overlap. The similar expression patterns seen in zebrafish and chick potentially reflect a broadly conserved role in early otic development. To explore the role of tfap2a, we characterized the effects of tfap2a loss of function or misexpression on neurogenesis in the otic vesicle in zebrafish. Disruption of tfap2a causes no overt defects in morphogenesis of the otic vesicle [27]. However, tfap2a-/- (lockjaw) mutants produced only half the normal number of ngn1-positive neuroblasts in the otic epithelium at 24 hpf, the stage when neurogenesis normally peaks in wild-type embryos (Fig. 2A, C). At later stages, too, tfap2a-/- mutants continued to show significant deficiencies in neuroblast specification (Fig. 2D, F, M). Similar results were seen in tfap2a morphants (tfap2a-MO, Fig. 2M). We next used a heat-shock inducible transgenic line, hs: tfap2a, to misexpress tfap2a at various developmental stages. Activation of hs: tfap2a at 20 hpf increased the peak number of neuroblasts in the otic vesicle at 24 hpf by 30% (58. 33±3. 21 ngn1+ cells in hs: tfap2a embryos vs. 44. 5±2. 65 cells in controls, Fig. 2B). Activation of hs: tfap2a at 24 hpf prolonged the phase of peak neurogenesis, resulting in twice the normal number of neuroblasts at 28 hpf (Fig. 2D, E, M). Despite the initial surge, however, the number of ngn1+ neuroblasts subsequently declined sharply in transgenic embryos, dropping below the level seen in control embryos at 30 hpf and thereafter (Fig. 2G, H, M). Because transgene activity decays 5 hours after heat shock (S1 Fig.), we tested the effects of serial heat shocks at 24 hpf and 29 hpf. This resulted in elevated neurogenesis through at least 32 hpf, when transgenic embryos had twice as many ngn1+ cells in the otic epithelium as in control embryos (Fig. 2G, I, N). Neurogenesis in the ear normally ceases by 42 hpf [5,8], prompting us to investigate whether termination of neurogenesis could be altered by later misexpression of tfap2a. Indeed, activation of hs: tfap2a at 38 hpf prolonged specification of neural precursors, as ngn1+ neuroblasts were still present in the otic vesicle through at least 43 hpf (Fig. 2J, K). In contrast, activation of hs: tfap2a at 40 hpf was not sufficient to prevent or delay the cessation of neurogenesis in the otic vesicle (Fig. 2L). Overall these results indicate that tfap2a enhances neurogenesis in the ear but cannot induce ectopic neurogenesis beyond the floor of the otic vesicle nor reactivate neurogenesis after it has stopped. We next examined whether altered levels of neuroblast specification caused by manipulating tfap2a function were followed by changes in later stages of neuronal differentiation. Normally, newly specified neuroblasts delaminate from the otic vesicle, lose expression of ngn1 and initiate expression of neurod, a marker of the “transit-amplifying” (TA) stage of development [1,5] (Fig. 1K). Surprisingly, despite reduced neurogenesis in tfap2a-/- mutants and tfap2a morphants, the number of neurod+ TA cells was greater than normal at every time point examined (Fig. 3B, E, H, J). Conversely, despite the large increase in neurogenic specification caused by overexpression of tfap2a at 24 hpf, the number of neurod-expressing TA cells was reduced at all time points through 48 hpf (Fig. 3C, F, I, J). We hypothesized that changes in the size of the TA pool reflect changes in the overall pace of neuronal differentiation. To test this we examined expression of Isl1, a marker of mature SAG neurons. We observed that tfap2a-/- mutants and tfap2a morphants produced fewer than normal neurons despite the increased number of neurod+ cells (Fig. 4E-H, T). The deficiency in neuronal maturation persisted in tfap2a-/- mutants through at least 72 hpf (S2 Fig.). In contrast, activation of hs: tfap2a had the opposite effect. Activation of hs: tfap2a during placodal stages elevated accumulation of Isl1+ neurons at 30 hpf, and the fold-stimulation was progressively increased with successively later stages of activation (Fig. 4M). Activation of hs: tfap2a at 24 hpf led to maximal accumulation of neurons, with nearly twice the normal number of Isl1+ neurons observed in transgenic embryos at 30 hpf and 37 hpf (Fig. 4I-L, M, T). Interestingly, transgene activation at these early stages enhanced accumulation of anterior (vestibular) SAG neurons but not posterior (auditory) neurons (Fig. 4D, L). However, activating hs: tfap2a expression at 29 hpf increased accumulation of both anterior and posterior neurons (Fig. 4N-P), consistent with our previous findings that auditory neurons are specified at later stages than vestibular neurons [5]. Together these data indicate that disruption of tfap2a inhibits neurogenesis and slows neural maturation, whereas misexpression of tfap2a stimulates neuroblast specification and accelerates subsequent differentiation. Importantly, although activation of hs: tfap2a at 24 hpf led to elevated accumulation of mature neurons through 37 hpf, the number of mature neurons fell dramatically thereafter to roughly half normal by 48 hpf (Fig. 4T). This decline was preceded by a marked increase in the rate of apoptosis amongst mature neurons (Fig. 4Q-S, U). Elevated cell death possibly reflects insufficient duration of earlier stages of differentiation and consequent misregulation of factors required for neuronal survival (see below). To determine whether the above changes in SAG development resulted from mis-patterning of the otic vesicle, we examined expression of several regional markers. In tfap2a-/- mutants and tfap2a morphants, domains of the dorsal marker dlx3b and the ventrolateral marker otx1b were slightly contracted (Fig. 5B, E). Domains of pax5, an anterior-ventral marker of the utricular macula, and pou3f3b, a posterior-medial marker of the saccular macula were not altered (Fig. 5H, K). Additionally, sensory epithelia appeared to develop normally and there were no obvious changes in hair cell development through 54 hpf (Fig. 5M). Activation of hs: tfap2a at 24 hpf led to weak contraction otx1b but a substantial expansion of the dlx3b (Fig. 5C, F). pax5 was expressed in its normal domain but at a reduced level (Fig. 5L). Expression of pou3f3b was normal (Fig. 5I) and there were no changes in accumulation of hair cells through 54 hpf (Fig. 5M). Thus, gross patterning in the otic vesicle was nearly normal in tfap2a-/- mutants and morphants, though substantial changes were seen in one marker (dlx3b) following overexpression of tfap2a. Such changes in gene expression likely reflect changes in cell signaling as described below. We next investigated whether Tfap2a activity influences Notch and Fgf signaling, pathways known to regulate development of SAG neurons. For example, Delta-Notch signaling is normally activated by neurogenic factors Ngn1 and Neurod and serves as a feedback inhibitor of neurogenesis [2,33,34]. Disruption of Delta-Notch signaling leads to excess neural specification and precocious differentiation [35], similar to the effects of misexpression of tfap2a. Here we observed that expression of deltaA and deltaB was increased in tfap2a-/- mutants and, conversely, delta gene expression was strongly impaired following overexpression of tfap2a (Fig. 6A-F). Similar changes were observed for the Notch target gene her4, which increased in tfap2a-/- mutants and decreased following activation of hs: tfap2a (Fig. 6G-I). Thus Tfap2a appears to inhibit Notch activity during development of SAG neurons by inhibiting expression of Notch ligands. Fgf signaling has a more complex role in neural development in the ear. At early stages specification of neuroblasts requires Fgf. As development proceeds, however, rising levels of Fgf5 secreted by mature SAG neurons terminates specification of new neuroblasts and delays differentiation of TA cells into mature neurons [5] (Fig. 1K). Weak impairment of Fgf signaling can prolong neurogenesis and accelerate neural differentiation, mimicking aspects of the tfap2a overexpression phenotype. We therefore examined expression of various fgf genes in the otic vesicle. Knockdown of tfap2a did not appear to alter expression of fgf3, fgf8, or fgf10a (Fig. 6K, N, Q). Activation of hs: tfap2a at 24 hpf led to reduced expression of fgf3 and fgf8, but expression of fgf10a was not altered (Fig. 6L, O, R). To look for changes in Fgf signaling, we examined expression of Fgf-target genes spry4, etv4 (pea3) and etv5b (erm). Although etv4 and etv5b expression appeared normal in tfap2a morphants (S3D, H Fig.), spry4 was expressed at higher levels and in a broader domain than normal in tfap2a-/- mutants, indicating that Fgf signaling was elevated (Fig. 6S-T). Conversely, activation of hs: tfap2a at 24 hpf reduced expression of etv4, etv5b and spry4 by 26 hpf, indicating a reduced level of Fgf signaling (Fig. 6U-V, S3A-J Fig.). Fgf signaling remained reduced through 28 hpf but started to recover after 30 hpf as transgene activity decayed (S3K-Q Fig.). Thus, Tfap2a appears to limit Fgf signaling, in part through reducing expression of fgf genes. To test whether Tfap2a can influence Fgf signaling independently of ligand expression, we co-misexpressed tfap2a and fgf8. While activation of hs: fgf8 led to global upregulation of etv5b, co-activation of hs: tfap2a with hs: fgf8 at 24 hpf partially suppressed expression of etv5b (Fig. 6W-X). This indicates that tfap2a can inhibit Fgf signaling at a level downstream of ligand accumulation. To test the functional significance of elevated Fgf signaling in tfap2a morphants, we examined whether weakly inhibiting Fgf signaling could rescue the neurogenic deficiencies in tfap2a morphants. Indeed, reducing the level of Fgf signaling via low-level activation of hs: dnfgfr1 (dominant-negative Fgf receptor) at 35°C restored ngn1+ cell counts to normal in tfap2a morphants (Fig. 6Y-Z). This suggests that elevated Fgf signaling partially accounts for the reduced neuroblast specification in tfap2a morphants and mutants. Because Tfap2a appears to dampen both Fgf and Notch signaling, we tested whether weakening both pathways by other means could mimic the effects of tfap2a overexpression. Low level activation of hs: dnfgfr1 at 36. 5°C increased the number of ngn1+ neuroblasts in the ear by ~30% (Fig. 7B, M). Similarly, reducing the level of Notch signaling by treatment with the gamma-secretase inhibitor LY411575 increased the number of ngn1+ cells in the otic vesicle by ~50% (Fig. 7C, M). Combining these conditions to reduce both Fgf and Notch signaling further increased neuroblast specification, closely mimicking the effects of activating hs: tfap2a at 24 hpf (Fig. 7D, M). Likewise, inhibiting both Fgf and Notch together reduced the number of neurod+ cells in the TA pool and increased accumulation of Isl1+ neurons through 37 hpf in a manner similar to activating hs: tfap2a at 24 hpf (Fig. 7E-O). Moreover, embryos inhibited for both Fgf and Notch signaling showed a dramatic loss of mature SAG neurons after 37 hpf, again mimicking the effects of hs: tfap2a activation (Fig. 7O). Interestingly, inhibition of either Fgf or Notch alone caused similar but more modest acceleration of neural differentiation, but such conditions did not lead to subsequent loss of mature neurons after 37 hpf (Fig. 7O). This is possibly because differentiation, though accelerated relative to control embryos, is still slow enough to allow expression of all factors essential for survival. Finally, activating hs: tfap2a at 24 hpf combined with conditions to inhibit Fgf and Notch did not further increase accumulation of Isl1+ neurons at 37 hpf (S4 Fig.). Thus, reducing both Fgf and Notch signaling is sufficient to recapitulate all observed effects of tfap2a overexpression. We considered the possibility that the ability of Tfap2a to alter development of SAG cells outside the ear could arise secondarily from perturbation of earlier developmental stages within the otic vesicle. To test whether Tfap2a can specifically influence cells after delamination (without altering early development within the otic vesicle), we activated hs: tfap2a at 40 hpf when neurogenesis has ceased in the otic vesicle. Recall that transgene activation fails to prolong or reinitiate neuroblast specification at this stage (Fig. 2L). Regardless, overexpression of tfap2a at 40 hpf still reduced the number of neurod+ cells and led to an increase in Isl1+ neurons at 50 hpf (Fig. 8B, F, M, N). Misexpression of tfap2a also reduced the total number of cells in the TA pool that incorporated BrdU (Fig. 8J, O). However, this was proportional to the reduction in the total number of neurod+ cells (Fig. 8P), consistent with acceleration of the entire TA pool. A notable difference from earlier activation is that activating hs: tfap2a at 40 hpf did not lead to apoptosis of SAG neurons at later stages, as the number of Isl1+ cells remained elevated and in fact continued to increase through at least 72 hpf (Fig. 8B, F, N). The same effects were obtained by directly inhibiting both Fgf and Notch signaling after 40 hpf (Fig. 8C, G, K M-P). Conversely, misexpressing Fgf8 and NICD (Notch intracellular domain) by heat shock activation at 40 hpf led to accumulation of more TA cells and fewer mature neurons than normal at 50 and 72 hpf, indicating a delay in neuronal differentiation (Fig. 8D, H, M, N). Moreover, activating Fgf and Notch together increased the percentage of neurod+ cells that continue to incorporate BrdU (Fig. 8L, O, P), suggesting that the majority of cells in the TA pool persist in a relatively immature stage of SAG development. Thus, manipulating tfap2a, or Fgf and Notch signaling directly, can alter the rate of differentiation of TA cells even when earlier development within the otic vesicle has occurred normally. On the other hand, survival of mature SAG neurons requires normal development at early stages. It is noteworthy that tfap2a is not normally expressed in the TA pool or mature neurons, yet knockdown or misexpression of tfap2a alters the rate of differentiation and survival of these cells. This raised the possibility that Tfap2a could act non-autonomously on cells outside the ear. To test directly whether tfap2a can act non-autonomously, we generated genetic mosaics by transplanting wild-type cells into hs: tfap2a host embryos. We reasoned that if hs: tfap2a were to act non-autonomously, activating the transgene in host cells should be able to prevent wild-type cells from responding to Fgf sources in the otic vesicle. In support, activation of hs: tfap2a at 24 hpf suppressed etv5b expression in the majority (73. 5%) of transplanted wild-type cells by 26 hpf (Fig. 9C-E), indicating that tfap2a acts non-autonomously to modulate the response to Fgf. In contrast, all wild-type donor cells transplanted into wild-type host embryos showed normal expression of etv5b in the otic vesicle (Fig. 9A, B, E). Thus Tfap2a non-autonomously inhibits Fgf signaling in the otic vesicle. Bmp signaling is well known to antagonize Fgf signaling in a variety of developmental contexts. Here we found that blocking Bmp signaling with the pharmacological inhibitor dorsomorphin (DM) [36] strongly suppressed the ability of hs: tfap2a to reduce Fgf signaling. For example, etv5b expression was nearly normal in the otic vesicle 2 hours after the activation of hs: tfap2a when embryos were also treated with DM (Fig. 9F, G). DM treatment alone had negligible effects on expression of etv5b (Fig. 9H). We next surveyed expression of various bmp genes following activation of tfap2a and identified bmp7a as a likely candidate for mediating its effects on Fgf signaling. bmp7a is normally expressed in cells at the anterior and posterior ends of the otic vesicle, and at a lower level in a ventrolateral domain that overlaps the tfap2a expression domain [37] (Fig. 9I-J, compare with Fig. 1C). Expression of bmp7a was nearly abolished in tfap2a-/- mutants (Fig. 9K). In contrast, activation of hs: tfap2a strongly upregulated bmp7a expression in the otic vesicle as well as in surrounding tissues (Fig. 9L). In contrast to bmp7a, we observed no consistent changes in expression of bmp2b or bmp4 following manipulation of tfap2a function (S5 Fig.), indicating that changes in bmp7a are relatively specific. Together, these data suggest that tfap2a positively regulates bmp7a, which in turn restricts the level of Fgf signaling during otic development. We next examined whether Bmp signaling mediates the effects of tfap2a on Notch activity. In support, blocking Bmp with DM rescued expression of deltaB and her4 following activation of hs: tfap2a at 24 hpf (Fig. 10A-L, M-P). Treatment with DM alone caused a slight but significant increase in the number of cells expressing deltaB and her4 (Fig. 10D, H, I-L). Thus the ability of tfap2a to restrict Notch signaling requires elevated Bmp signaling. Finally, we tested whether the effects of tfap2a on SAG development also require Bmp signaling. Blocking Bmp after activating hs: tfap2a at 24 hpf restored neuroblast specification to normal in 6 out of 8 specimens (Fig. 10I-L, Q). Similarly, accumulation of mature Isl1+ neurons was nearly normal in 12 out of 15 embryos at 30 hpf (Fig. 10R) and was restored to normal in all specimens at 37 hpf (n = 15) (Fig. 10S). DM treatment alone caused a slight but significant reduction in neuroblast specification and accumulation of mature SAG neurons (Fig. 10Q-S). Together, these findings support a model in which tfap2a regulates the level of bmp7a expression in the otic vesicle, which in turn restricts Fgf and Notch signaling to control the amount, duration and speed of SAG development.
The interplay between Fgf and Notch is complex and dynamic, and levels must be precisely balanced for proper development of the SAG. A low-to-moderate level of Fgf is required to initiate neurogenesis by activating expression of ngn1 [5,7, 38–40], which in turn activates expression of Notch ligands [2,34]. Notch activity serves to limit and slow neurogenesis [33,34]. Neurogenesis is also inhibited at later stages by rising levels of Fgf5 derived from mature neurons [5] (Fig. 1K). Without proper modulation by tfap2a, both Fgf and Notch signaling quickly become overactive, which terminates specification prematurely and impedes maturation of neuroblasts in the TA pool, thereby leading to under-production of mature SAG neurons. While too much Fgf and Notch activity clearly impairs neurogenesis, insufficient levels are ultimately far more damaging to SAG development. Conditions that reduce Fgf and Notch signaling (e. g. overexpression of tfap2a) cause dramatic acceleration of differentiation and overproduction of neurons, most of which later die upon maturation. Neuronal death appears to arise secondarily from acceleration of early stages within the otic vesicle, as accelerating later stages does not lead to neuronal death. It is likely that early neuroblast differentiation is especially sensitive to acceleration due to insufficient buildup of factors needed for survival and function of mature neurons. In contrast, an important attribute of the TA pool is that the rate of differentiation can be regulated without compromising subsequent neuronal survival. The TA pool represents a relatively stable population of slowly cycling progenitors that must be maintained to meet the needs of the growing larva or to regenerate new neurons following damage [5]. It is likely that tfap2a is needed only transiently in the otic vesicle to prolong specification and establish a healthy progenitor population. Once neuroblast specification has terminated, however, downregulation of tfap2a appears necessary to allow Fgf and Notch signaling to rise sufficiently to balance rates of proliferation vs. differentiation in the TA pool. We have shown for the first time a role for Bmp in SAG development. Numerous earlier studies have shown that Bmp regulates morphogenesis of semicircular canals [41–44] and numerous aspects of development of sensory epithelia [45–49]. Bmp has also been found to promote SAG survival and neurite outgrowth in chick explant cultures [50]. However, no previous studies have detected a role in specification or differentiation of SAG neurons. Abello et al. [38] reported that blocking Bmp signaling did not alter the size of the neurogenic domain in the chick otic vesicle; and mosaic misexpression of an activated form of Bmp receptor did not inhibit neurogenesis, though the possibility that it may have accelerated neurogenesis was not examined. In comparison, we find that tfap2a activates expression of bmp7a and that blocking Bmp signaling reverses the effects of tfap2a overexpression. Treating wild-type embryos with DM partially mimics the effects of disrupting tfap2a. The more severe defects caused by tfap2a loss of function could indicate that additional factors help mediate the effects of Tfap2a. Alternatively, Bmp7a could act partly through non-canonical signaling, similar to the role of Bmp7 in establishing tonotopy in the organ of Corti in mouse [46]. The specific requirement for Bmp7a in SAG development cannot be assessed by examining bmp7a mutants because development of the otic placode is severely compromised [51]. Development of lines to conditionally disrupt or misexpress bmp7a could resolve many of these issues. Interestingly, in zebrafish Bmp effectors Smad1 and Smad5 are specifically upregulated in delaminated SAG cells [37,52] (Fig. 1K). This constitutes an unusual form of regulation because smad1/5 genes are broadly expressed with relatively little variation in the level of expression. Elevated levels of Smad1/5 accumulation could render SAG precursors outside the ear especially sensitive to Bmp, explaining how Bmp expressed within the otic vesicle could have such a profound effect on development of TA cells. We do not yet know how tfap2a is regulated in the otic vesicle. We detect no changes in expression after manipulating levels of Fgf, Notch, Bmp, Wnt or ngn1. It is possible that expression of tfap2a in the otic placode and otic vesicle reflects auto-regulatory maintenance from earlier stages. During gastrulation tfap2a is induced by Bmp in non-neural ectoderm where it functions as a competence factor for preplacodal development [9]. Once induced Tfap2a acts to maintain its own expression even if Bmp is subsequently blocked [11]. This is an important aspect of regulation because dorsally expressed Bmp-antagonists are required to initiate preplacodal development near the end of gastrulation [9,53–55]. Although expression of tfap2a could simply persist in the otic placode through self-maintenance, it is not clear how expression becomes restricted to ventrolateral cells in the otic vesicle. A similar pattern is seen in the chick otic vesicle, possibly indicating a conserved mechanism ([56]; Fig. 1). Identifying factors that regulate tfap2a in zebrafish and chick will likely shed light on general mechanisms of otic patterning and specific mechanisms of otic neurogenesis.
The wild type strains were derived from AB line (Eugene, OR). Transgenic lines used in this study include Tg (hsp70: tfap2a) x24 [11], Tg (hsp70: fgf8a) x17 [57], Tg (hsp70I: dnfgfr1-EGFP) pd1 [58], Tg (hsp70I: gal41. 5) kca4 [59], Tg (UAS: myc-Notch1a-intra) kca3 [60] and Tg (brn3c: gap43-GFP) s356t [61]. These transgenic lines are referred as hs: tfap2a, hs: fgf8, hs: dnfgfr1, hs: gal4/UAS-NICD and brn3c-GFP respectively. Mutant line tfap2am819 [62] was used for most loss of function studies. Mutants were identified by characteristic phenotypes showing expected Mendelian frequencies. Except where noted, embryos were maintained at 28. 5°C in fish water containing 0. 008% Instant Ocean salts, methylene blue and PTU (1-phenyl 2-thiourea, 0. 3 mg/ml, Sigma) to block melanin formation. Embryos were staged according to standard protocols [63]. To activate heat-shock inducible transgenic lines, heterozygous transgenic embryos were incubated at 39°C for 30 minutes except where noted. Under this condition, activation of hs: tfap2a at 24 hpf led to a detectable increase in tfap2a levels by the end of the heat-shock period. Maximal tfap2a expression was seen at 25. 5 hpf and the tfap2a levels remained elevated in the otic vesicle thorough at least 29 hpf (S1 Fig.). Since the complete blockage of Fgf signaling inhibits neurogenic specification in the otic vesicle [5], weak attenuation of Fgf signaling was achieved by activation of hs: dnfgfr1 at 35°C for 30 minutes (Fig. 6) or at 36. 5°C for 30 minutes (Fig. 7). After the heat-shock, embryos were incubated at 33°C until fixation. In some loss of function experiments, tfap2a was knocked down by injecting embryos at the 1-cell stage with approximately 5 ng of tfap2a morpholino oligomer (MO). The sequence of tfap2a MO has been tested previously for specificity and efficiency [64]. Notch signaling was blocked by treating embryos with LY411575 diluted from a 10 mM stock in DMSO to a final concentration of 30 μM in fish water. Bmp signaling was blocked by Dorsomorphin (Sigma, P5499) diluted from a 10 mM stock solution into a final concentration of 100 μM in fish water. Treatments were carried in a 24-well plate with a maximum of 15 embryos per well in a volume of 500 μl each. In situ hybridization and antibody staining was carried out as described previously [65–67]. In situ TUNEL assay was performed by using Promega terminal deoxynucleotidyl transferase (M828A) according to the manufacturer’s protocol. Following primary and secondary antibodies were used in this study: anti-Islet1/2 (Developmental Studies Hybridoma Bank 39. 4D5,1: 100 for whole-mount, 1: 250 for cryo-sections), anti-BrdU (Beckton-Dickinson, 1: 250) and Alexa 546 goat anti-mouse IgG (Invitrogen A-11003,1: 50 for whole-mount, 1: 250 for cryo-sections). Cryo-sectioning and BrdU labeling were carried out as described previously [5]. Whole-mount stained embryos were sectioned except for Figs. 1,7 and S4 where anti-islet1/2 staining was performed on sections using standard whole mount protocols. Wild-type donor cells were injected with the lineage tracer (tetramethylrhodamine labeled, 10,000 MW, lysine-fixable dextran in 0. 2 M KCl) and transplanted into non-labeled hs: tfap2a embryos at blastula stage. Embryonic day 3 (E3) and E4 chick embryos were fixed and embedded in gelatin (7. 5% gelatin, 15% sucrose in PBS). 14μm thick sections were collected on Superfrost Plus slides. For AP2a and Jagged-1 co-detection, slides were boiled in 10mM citric acid for 10 minutes prior to antibody application and then incubated in 0. 012% hydrogen peroxide for 15 minutes at room temperature. The 3B5 AP2α monoclonal antibody developed by Trevor Williams was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology, Iowa City, IA 52242. AP2α antibody was diluted 1: 100 and Jagged-1 polyclonal antibody (Santa Cruz Biotechnology H-114) was diluted 1: 200 in blocking buffer (PBS with 0. 02% Tween-20,0. 1% Triton X-100, and 10% goat serum). Staining was detected with biotinylated mouse secondary antibody (Mouse Vectastain ABC kit) in conjunction with PerkinElmer TSA Plus Cyanine-3 System and AlexaFluor 488 conjugated rabbit secondary antibody diluted 1: 500 in A+B substrate solution (AlexaFluor goat anti-rabbit, Invitrogen). All slides were mounted in Fluoromount G (Southern Biotech). For pairwise comparisons, student’s t-tests were used to evaluate significance. For experiments involving more than two groups significance was evaluated using one-way ANOVA and Tukey post-hoc HSD tests. The studies described herein were fully compliant with federal guidelines and IACUC-approved Animal Use Protocol number 2012–011.
Neurons of the statoacoustic ganglion (SAG) transmit impulses from the inner ear necessary for hearing and balance. SAG cells exhibit a complex pattern of development, regulation of which remains poorly understood. Here we show that transcription factor Tfap2a coordinates multiple cell signaling pathways needed to regulate the quantity and pace of SAG neuron production. SAG progenitors originate within the developing inner ear and then migrate out of the ear towards the hindbrain before forming mature neurons. We showed previously that Fgf initiates formation of SAG progenitors in the inner ear, but rising levels of Fgf signaling eventually terminate this process. Elevated Fgf also stimulates proliferation of SAG progenitors outside the ear and delays their maturation. Notch signaling is also known to limit SAG development. Tfap2a governs the strength of Fgf and Notch signaling by activating expression of Bmp7a, which inhibits Fgf and Notch. Together these signals stabilize the pool of SAG progenitors outside the ear by equalizing rates of maturation and proliferation. This balance is critical for sustained accumulation of SAG neurons during larval growth as well as regeneration following neural damage. These findings could inform development of stem cell therapies to correct auditory neuropathies in humans.
Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling