Proteome balance is safeguarded by the proteostasis network (PN), an intricately regulated network of conserved processes that evolved to maintain native function of the diverse ensemble of protein species, ensuring cellular and organismal health. Proteostasis imbalances and collapse are implicated in a spectrum of human diseases, from neurodegeneration to cancer. The characteristics of PN disease alterations however have not been assessed in a systematic way. Since the chaperome is among the central components of the PN, we focused on the chaperome in our study by utilizing a curated functional ontology of the human chaperome that we connect in a high-confidence physical protein-protein interaction network. Challenged by the lack of a systems-level understanding of proteostasis alterations in the heterogeneous spectrum of human cancers, we assessed gene expression across more than 10,000 patient biopsies covering 22 solid cancers. We derived a novel customized Meta-PCA dimension reduction approach yielding M-scores as quantitative indicators of disease expression changes to condense the complexity of cancer transcriptomics datasets into quantitative functional network topographies. We confirm upregulation of the HSP90 family and also highlight HSP60s, Prefoldins, HSP100s, ER- and mitochondria-specific chaperones as pan-cancer enriched. Our analysis also reveals a surprisingly consistent strong downregulation of small heat shock proteins (sHSPs) and we stratify two cancer groups based on the preferential upregulation of ATP-dependent chaperones. Strikingly, our analyses highlight similarities between stem cell and cancer proteostasis, and diametrically opposed chaperome deregulation between cancers and neurodegenerative diseases. We developed a web-based Proteostasis Profiler tool (Pro2) enabling intuitive analysis and visual exploration of proteostasis disease alterations using gene expression data. Our study showcases a comprehensive profiling of chaperome shifts in human cancers and sets the stage for a systematic global analysis of PN alterations across the human diseasome towards novel hypotheses for therapeutic network re-adjustment in proteostasis disorders.
Eukaryotic proteomes comprise a complex repertoire of diverse protein species that are organized in a modular interactome network in order to execute native function in support of proteostasis and a healthy cellular phenotype. Proteome balance is safeguarded by the proteostasis network (PN), an intricately regulated network of conserved processes that have evolved to safeguard the healthy folded proteome [1]. Cellular proteostasis capacity is limited within the constraints of each cell’s proteostasis boundary [2]. Proteostasis imbalances, deficiency and functional collapse are implicated in a broad and increasing spectrum of protein conformational diseases with loss of native function or gain of toxic function, ranging from metabolic and neurodegenerative diseases to cancer [3,4]. Increasing awareness of the fundamental role of the PN in cellular health, its relevance in diseases and potential as a therapeutic target of proteostasis regulator (PR) drugs call for a systematic and systems-level assessment of PN deregulation throughout the human diseasome, towards improved understanding of diseases of proteostasis deficiency and rationalized network-informed approaches to therapeutic proteostasis re-adjustment. Important progress has been made in our understanding of proteostasis biology, building on fundamental insights on conserved proteostasis processes and their role in disease, such as chaperone-assisted protein folding and quality control [5–8], clearance through autophagy [9–13] and the ubiquitin-proteasome system (UPS) [14–16], followed by the appreciation of their concerted action within a conserved tightly regulated PN [1,17]. The identification, development and first clinical evaluations of small molecule PR drugs for therapeutic re-adjustment of proteostasis diseases such as cystic fibrosis represents a novel and powerful therapeutic paradigm [1,2, 18–23]. First investigations have started to explore systems-level quantitative and functional approaches to assess the implications of PN functional arms such as the chaperome in human tissue aging and disease [4,24–26]. A precise understanding of the molecular mechanisms by which PN alterations contribute to disease could open novel therapeutic intervention strategies in a wide spectrum of proteostasis-related diseases. Still, to date, there has been no systematic study addressing the characteristics and extent of PN alterations in human diseases at a systems-level. The folding functional arm, the human chaperome, is highly conserved and of central importance in the PN, responsible for maintaining the native folded proteome. In cancers, mutations and genomic instability inevitably entail alterations of proteome composition and balance that are far less well explored than the consequences of nucleic acid sequence alterations. Post-translational alterations at the proteomic level are beyond the reach of DNA repair mechanisms and cancer cells are constantly challenged by the need to accommodate large amounts of proteotoxic stress in consequence of increased translational flux and proliferation as well as proteotoxic stressors. Proteome instability and pathological alterations in the abundance of key signalling or housekeeping molecules such as kinases, metabolic enzymes or molecular transporters have to be buffered by the PN to ensure cellular survival. The cancerous state poses characteristic requirements on the PN, such as high chaperone levels and elevated proteasome activity in order to ensure for sufficient correction or elimination of aberrant protein species in light of increased translational flux and metabolic stress [27]. This chronic challenge ultimately drives cancer cells into a dependency on quality control and stress response mechanisms, a phenomenon previously described as non-oncogene addiction [28,29]. Several individual chaperones and heat shock proteins such as HSP90 have consistently been found upregulated in cancers [27]. However, the profile and extent of chaperome differential expression has not been assessed systematically across the human cancer landscape. Challenged by the genetic complexity and heterogeneity, collective prevalence and unmet medical need of the wide spectrum of human cancers as well as the lack of a systems-level understanding of proteostasis alterations during carcinogenic transformation, we developed a novel integrated analytical pipeline and software toolkit for the quantitative profiling of chaperome changes across the human cancer landscape (Fig 1). We utilized an expert-curated functional chaperome ontology comprising the ensemble of 332 human chaperone and co-chaperone genes [4] (Fig 1A). In order to apply our analytical workflow on a recent and comprehensive cancer gene expression dataset with clinical relevance, we turned to The Cancer Genome Atlas (TCGA) compendium [30]. We started with a customized genomic analysis pipeline in order to map chaperome functional family expression changes across TCGA solid cancers compared to matching normal tissue (Fig 1B). The resulting top-level view on cancer chaperome deregulation revealed a broad chaperome upregulation throughout the majority of cancers. This consistent and high overall chaperome upregulation prompted us to zoom in on functional sub-families. This analysis surfaced clusters of chaperome functional family up- and downregulation signatures that enabled further stratification of cancers. In summary, our analysis of the 10 major chaperome functional families reveals pronounced tissue differences of cancer chaperome deregulation. The preferential upregulation of ATP-dependent chaperone families such as HSP90s and HSP60s, while ATP-independent chaperones, co-chaperones, and small heat shock proteins (sHSPs) are consistently downregulated, is opposed to chaperome alteration patterns observed in brain tissues during aging and in neurodegenerative diseases [4]. These characteristic chaperome-wide differences further justify our approach and need for systematic maps of PN deregulation across the human diseasome. In order to enable comprehensive, contextual, and quantitative representations of the complexity of chaperome alterations across a large number of patient biopsy disease datasets, we developed a new custom data dimensionality reduction and visualisation approach. Combining Meta-PCA, a novel principal component analysis (PCA) based two-step dimension reduction algorithm and its resulting quantitative M-scores of chaperome functional family disease alteration of gene expression with contextual polar plot visualisations, we provide intuitive quantitative maps of cancer chaperome gene expression changes (Fig 1B). The mechanistic understanding of genotype-phenotype relationships in complex genetically heterogeneous diseases such as cancers requires the consideration of the cellular interactome network [31]. To reduce complexity and to highlight contextual changes of chaperome functional families, we first generated a custom curated high-confidence physical protein-protein interaction (PPI) chaperome network. We then collapsed proteins (nodes) and physical PPIs (edges) into a meta-network, where meta-nodes represent respective functional family members and meta-edges bundle the interactions between families (Fig 1C). This curated high-quality chaperome meta-network base-grid enabled the contextual projection of cancer-specific chaperome functional family differential gene expression (M-scores) onto the underlying interactome. We integrated these dimensions into interactome-guided, three-dimensional topographic maps visualising chaperome functional family cancer differential gene expression changes in the context of interactome network proximity, intuitively providing quantitative views of cancer chaperome deregulation (Fig 1D). To make these resources easily available to the community, we developed Proteostasis Profiler (Pro2), an integrated web-based suite of applications enabling intuitive quantitative analyses and comparative visualisation of differential expression of complex PN alterations across large disease dataset compendia such as the TCGA. Visualisation and analysis features include heat map clustering and polar plot display. Integrated meta-networks and interactome-guided 3D topographic maps ease comparative exploration of cancer chaperome deregulation in the context of interactome network wiring. Pro2 is designed to serve the scientific community as a user-friendly application for systems-level exploration of PN disease alterations, at reduced complexity. Overall, this study represents a systematically derived systems-level atlas of chaperome deregulation maps in cancers and neurodegenerative diseases, with a detailed focus on chaperome functional family alterations. The integrated genomic analysis workflow, built into the Pro2 suite of visualisation tools, provides a resource and analytical platform for future characterisation and exploration of PN deregulation patterns across the human diseasome, and as a readout interface for network shifts induced by therapeutic regulation.
Homeostasis of the cellular proteome, or proteostasis, is fine-tuned by the proteostasis network (PN), an intricately regulated network of conserved processes that have evolved to safeguard the native functional proteome and cellular health. The human chaperome, an ensemble of 332 chaperones and co-chaperones, represents a central functional arm within the PN in charge of maintaining the cellular folding landscape (S1A Table) [4]. Motivated by the genetic heterogeneity of cancers, their prevalence and associated medical need as well as the lack of a systems-level understanding of the role of proteostasis genomic alterations during carcinogenesis, we systematically assessed chaperome gene expression changes across the diverse spectrum of human cancers. We focused on an established resource of human cancer patient biopsy RNA-seq datasets provided through The Cancer Genome Atlas (TCGA) [30,32]. We considered 22 human solid cancers with available corresponding healthy counterpart tissue biopsy data. To obtain global views on chaperome commonalities or differences between cancers, we applied Gene Set Analysis (GSA) in order to quantify gene expression changes of the chaperome and its functional families. GSA is an advanced derivative of Gene Set Enrichment Analysis (GSEA) that methodologically differs primarily through its use of the maxmean statistic, the mean of the positive or negative gene scores in each gene set, whichever is larger in absolute value, that has proven superior to the modified Kolmogorov-Smirnov statistic used in GSEA [33]. Secondly, GSA uses a different null distribution for false discovery rate (FDR) estimations, through a restandardization of genes in addition to sample permutation in GSEA. This step is crucial, as it allows assessing statistical robustness of the expert-curated chaperome functional ontology gene family groups. We obtained the GSA derived probability (p values) for each functional gene group to be significantly up- or downregulated in cancer as ∆GSA values in the interval [-1, +1] according to ( (1—upregulation p value) — (1—downregulation p value) ). Notably, the human chaperome is predominantly upregulated across the majority of TCGA solid cancers with a ∆GSA group mean change of +0. 50 as compared to 100 random sets of non-chaperome genes (Fig 2A). This overall chaperome upregulation highlights cellular non-oncogene addiction to chaperone-assisted folding and protein quality control mechanisms in consequence of increased client load, further challenging cellular proteostasis and driving “proteostasis addiction” in cancers [34]. Despite the diverse established knowledge about the role of chaperone upregulation in cancer, the deregulation of the human chaperome has not been assessed at a systems-level throughout the human cancer landscape. To functionally resolve the general chaperome upregulation across cancers, we zoomed in on functional family gene expression alterations. GSA followed by Euclidean clustering of chaperome functional families revealed characteristic cancer differences. We found the key ATP-dependent HSP90 and HSP60 families, of which selected members have previously been shown to be upregulated in cancers, amongst the most highly upregulated functional families with ∆GSA group mean changes of +0. 55 and +0. 51, respectively, alongside ER-specific chaperone factors (+0. 53), followed by Prefoldins (PFDs, +0. 40), HSP100 AAA+ ATPases (+0. 32), and mitochondria-specific chaperones (MITOs, +0. 09) (Fig 2B). These six functional families of predominantly ATP-dependent chaperones represent an upregulation cluster with an overall group mean change of +0. 40. Intriguingly, the HSP70-HSP40 system and the large family of TPR-domain containing co-chaperones are overall repressed, with less consistent and largely cancer-specific alterations. HSP40 co-chaperones (-0. 09) cluster closest with HSP70s (-0. 10), indicative of the functional relationship they engage in during the HSP70 chaperone cycle. While HSP40 co-chaperones are overall weakly downregulated (-0. 09), also the second group of co-chaperones, the TPR-domain containing proteins, clustered with the HSP70-HSP40 system and were overall downregulated (-0. 18). Strikingly, sHSPs (-0. 74) were overall very consistently and strongly downregulated. Overall, sHSPs, TPRs, and the HSP70-HSP40 system clustered in a downregulation cluster with an overall group mean change of -0. 28 across cancers. Besides marked differences in the pattern of cancer functional family changes, Euclidean clustering of cancer groups (rows) revealed two major clusters (Fig 2B) (with a p-value equal to 0. 016 via multiscale bootstrap resampling). The vast majority of cancers is characterised by the consistent upregulation of HSP90s, ER-specific chaperones, HSP60s, PFDs, HSP100s and MITOs, opposed by a very consistent downregulation of sHSPs and a more cancer-specific overall downregulation of the HSP70-HSP40 system an TPR-domain co-chaperones. This group comprises Cluster I, representing ~91% of cancers, while Cluster II comprises ~9% of cancers with largely opposed chaperome deregulation signatures, in this set namely skin cutaneous melanoma (SKCM), and pheochromocytoma and paraganglioma (PCPG). In summary, systematically assessing gene expression data derived from a total of 10,456 patient samples uncovers broad differences in chaperome-scale deregulation across the variety of human solid cancers. While the vast majority of cancers shows consistent and strong upregulation of chaperome genes, this analysis reveals marked clusters of chaperome functional family expression signatures that further stratify cancers by differential chaperome expression. The major ATP-dependent chaperone functional families are consistently upregulated across a majority of cancers, while co-chaperones and sHSPs are consistently repressed (Fig 2B). In order to quantify this trend, we assessed the 22 differentially regulated cancer chaperomes for functional characteristics. First, we compared expression of 88 chaperones against 244 co-chaperones represented in the human chaperome [4]. Projecting TCGA cancer groups by their chaperone and co-chaperone differential expression highlights a significant preponderance of cancer chaperome upregulation, including both chaperones and co-chaperones, while only a minor fraction of each is downregulated (S2A and S2B Fig). Overall, chaperones tend to be more upregulated than co-chaperones (S2B Fig). Consistently, within the group of chaperones, we find an overall preponderance of upregulation of both ATP-dependent and ATP-independent chaperones, while only small fractions each are downregulated (S2C and S2D Fig). The 50 ATP-dependent chaperones are more upregulated than the 38 ATP-independent chaperones, while ATP-independent chaperones are more downregulated than ATP-dependent chaperones (S2D Fig). This analysis exposes a sub-group of cancers as notable exceptions to these trends, suggesting fundamental differences in chaperome deregulation. Projection of TCGA cancer groups by chaperone and co-chaperone up- and downregulation lends support for two groups of cancers, Group 1 and Group 2 (Fig 3A). These groups are recapitulated when projecting cancers by up- and downregulation of ATP-dependent versus ATP-independent chaperones (Fig 3C). K-means clustering confirms the significant separation of the Group 2 cancers pheochromocytoma and paraganglioma (PCPG), thyroid carcinoma (THCA), and the three kidney cancers kidney chromophobe (KICH), kidney renal papillary cell carcinoma (KIRP), and kidney renal clear cell carcinoma (KIRC) from Group 1 cancers, with a median silhouette width of s = 0. 63 (Fig 3A) and s = 0. 68 (Fig 3C). Group 1 cancers (red) represent the majority of cancers, characterized by strong overall chaperome upregulation, with low chaperone and co-chaperone repression, and a trend for upregulation of ATP-dependent chaperones (Fig 3B and 3D). Five Group 2 cancers however partition more distantly, with a lack of chaperome upregulation (Fig 3A and 3C). These cancers include three different kidney cancers, KICH, KIRP, and KIRC, which consistently lack chaperome upregulation (Fig 3A and 3B). Also, ATP-dependent chaperones are not preferentially upregulated in kidney cancers. Rather, an inverse trend is observed with increased downregulation of ATP-dependent chaperones (Fig 3C and 3D). Notably also, pheochromocytoma and paraganglioma (PCPG), rare related tumors of orthosympathetic origin, similarly show an even more prominent inverse alteration, with a preponderance of overall chaperome downregulation and preferential downregulation of ATP-dependent chaperones. Pheochromocytomas originate in the adrenal medulla, with close spatial association to the kidney, whose cancers are also Group 2 cancers. THCA is similar to PCPG, with a preferential downregulation of the chaperome and both PCPG and THCA represent tumors forming from cells of neuroendocrine origin. Collectively, the data point to a preferential upregulation of ATP-dependent chaperones in the majority of cancers, which we refer to as Group 1 cancers, with general differences in chaperome deregulation in Group 2 cancers, comprising kidney cancers and cancers of neuroendocrine origin, such as PCPG and THCA. Human embryonic stem cells (hESCs) are characterized by their capacity to replicate infinitely in culture, while maintaining a pluripotent state [35]. This immortal, undifferentiated phenotype resembles hallmark features of cancer cells such as an elevated global translational rate [36] and is expected to demand increased PN capacity capable of buffering imbalances to maintain proteostasis. Given the “stemness” phenotype of cancer cells and their resemblances with pluripotent stem cells we hypothesized that the consistent chaperome upregulation in cancers acts to mimic an enhanced stem cell PN setup. Increased proteasome activity [37] and elevated overall levels of the TRiC/CCT complex [38], representatives of the clearance and folding functional arms of the PN, respectively, have recently been associated with the intrinsic PN of pluripotent stem cells that acts to support their identity and immortality. It can be hypothesized that increased levels of central PN processes in stem cells exemplify characteristics of an enhanced PN setup. We thus assessed to which extent this stem cell PN setup is recapitulated in cancers. First, we assessed differential changes of the proteasome across TCGA cancers and observed an overall consistent upregulation of the 43 proteasomal genes (HGNC Family ID 690) in > 70% of cancers (Fig 4A, S1B Table) [39], matching the role of increased proteasomal activity for proteome maintenance in stem cells [37]. Notably, Group 1 and Group 2 cancers, which are specifically defined based on chaperome differential expression signatures (Fig 3), do not co-partition with cancer clusters obtained by proteasome differential expression (Fig 4A). Next, we assessed cancer differential expression of the eukaryotic chaperonin TRiC/CCT, a hetero-oligomeric complex of two stacked rings with each eight paralogous subunits representing the cytoplasmic ATP-driven HSP60 chaperones in charge of folding approximately 10% of the proteome [40]. TRiC/CCT is highly conserved and essential for cell viability [40]. Loss of complex subunits induces cell death and a decline of pluripotency of hESCs and induced pluripotent stem cells (iPSCs) [38]. Within the PN, TRiC/CCT mediated folding and autophagic clearance act in concert to prevent aggregation [41]. TRiC/CCT levels decline during stem cell differentiation, and CCT8 acts as complex assembly factor [38]. Intrigued by the finding that CCT8 is the most highly elevated subunit in stem cells and likely acting as assembly factor [38], we assessed differential expression of individual TRiC/CCT subunits across cancers. Hierarchical clustering of subunit expression across solid cancers highlighted CCT8 as highly consistently upregulated across all cancers (mean change = 0. 76, t test), and as overall second most highly upregulated subunit besides CCT6A (mean change = 0. 786, t test) (Fig 4B). Consistent with the overall preferential downregulation of ATP-dependent chaperones observed in Group 2 cancers (Fig 3), we found these cancers to cluster together with overall lowest TRiC/CCT expression, where PCPG stands out with a consistent downregulation of all subunits (Fig 4B). Together these findings suggest that proteostasis shifts in cancer cells add to an altered, enhanced PN state that mimics the immortal and resilient stem cell phenotype, buffering genome instability and ensuing proteomic imbalances in support of sustained and increased cellular proliferation throughout cancerogenesis. Overall chaperome upregulation in cancers, with preferential enrichment for upregulation of ATP-dependent chaperones, alongside consistent downregulation of sHSPs, is diametrically opposed to chaperome deregulation trends previously observed in a study of chaperome alterations in human aging brains and in patient brains with age-onset neurodegenerative diseases [4]. While sHSPs were the only chaperome family found significantly induced in brain aging and the age-onset neurodegenerative diseases Alzheimer’s (AD), Huntington’s (HD), and Parkinson’s (PD) disease, this family is consistently downregulated across cancers (Fig 2B). This opposed chaperome deregulation points towards characteristic and fundamental differences in PN deregulation between disease families. To investigate this disease group difference further, we applied the analysis outlined for cancers above also on the gene expression datasets that had earlier revealed global chaperome repression in AD, HD, and PD [4]. Our analysis reproduced the human chaperome as overall downregulated across AD, HD, and PD as compared to random permutations of non-chaperome genes (-0. 20 ∆GSA group mean change, Fig 5A). Delving deeper into chaperome functional subfamilies, we reproduce earlier findings reporting broad repression of the major chaperome functional families except for sHSPs, the only family found strongly upregulated (+0. 76) accompanied by slight upregulation of ER-specific chaperones (+0. 21) and TPRs (+0. 13) (Fig 5B). Thereby, our analytical workflow reproduces previously observed trends obtained in independent analyses, with different methods. With strong sHSP repression and upregulation of the HSP90, ER, HSP60, PFD, HSP100 and MITO chaperone families, cancers and neurodegenerative diseases display markedly diametrically opposed chaperome deregulation, not only at the overall chaperome-level (Fig 5C), but also with respect to alteration trends of chaperome functional families, where 70% of functional groups are altered in opposite directions (Fig 5D). These opposing chaperome deregulation signatures are in line with differing implications of proteostasis alterations in these diseases. While broad chaperome repression and proteostasis functional collapse is associated with aggregation and cytotoxicity of chronically expressed misfolding-prone proteins in neurodegenerative diseases [4], enhanced proteostasis buffering capacity is associated with “stemness”, immortality and proliferative potential of both stem and cancer cells [27]. Indeed, epidemiological evidence suggests an inverse correlation between cancers and neurodegenerative diseases [45–48], supportive of a potential mechanistic link between opposed chaperome deregulation and the molecular underpinnings of the two disease groups. These global differences in chaperome deregulation call for a systematic and quantitative assessment of PN deregulation dynamics in human diseases. In light of the diverse signatures of differential chaperome deregulation observed across cancers (Fig 2), and motivated by the increasing amount of genomics datasets available for cancers and other human diseases, we aimed at reducing data complexity by extracting quantitative indicators of chaperome differential cancer gene expression alterations, in order to gain insights through reduced complexity while retaining maximum information content. We devised Meta-PCA, a novel principal component analysis (PCA) based semi-supervised two-step dimension reduction approach that facilitates stratification of cancer patient and normal control samples within heterogeneous gene expression datasets (Fig 1B). Based on previous work on dimensionality reduction of heterogeneous gene expression datasets [49,50], we hypothesized that the underlying information contained in each chaperome functional group has a low dimensionality (meaning the functionality of each chaperome group can be quantified using only few, possibly one, variable) and could be surfaced using PCA, if there were sufficient samples available to represent the complete heterogeneity of chaperome alterations in cancers. Compared to conventional PCA, our method can deal with the effect of different group sizes, which as a confounder would negatively affect PCA results. Compared to simply calculating mean expression values of each functional group’s genes, Meta-PCA considers a wider range of gene expression information inherent to each functional group, resulting in scores with higher resolution. This could also be achieved by fully supervised methods such as Linear Discriminant Analysis. However, this case requires use of a method, which is blind to sample annotations so that these can later be used for validation, such as the unsupervised classification of cancer tissue type. Meta-PCA first uses tissue-wise PCA analyses to separate cancerous from control samples for individual tissues and maps functional family group gene expression changes to the global, or “meta”, mean expression change across cancers in order to obtain M-scores as quantitative indices of relative disease gene expression change (Eq 1). Inherent to the Meta-PCA method, patient-specific genomic variability is averaged out through the use of Meta-PCs derived from the TCGA collection of cancer biopsy samples, yielding mean reference boundaries. Assessing quantitative M-scores obtained through Meta-PCA against differential gene expression (∆GSA) obtained via GSA (Fig 2), we observed an overall significant Pearson correlation of 0. 61 (S1 Fig). In addition to general comparability between results obtained through both methods, Meta-PCA analysis reduces complexity while retaining genomic information. Therefore, we focus on Meta-PCA for quantitative representation of proteostasis alterations in human diseases. We plot differential chaperome changes as M-scores for all cancer samples and chaperome functional families simultaneously using polar plots, such that axes represent functional families or sub-groups (Fig 2B). We obtain the mean of all biopsy samples as reference boundaries for healthy (blue line) and cancer groups (red line) and include the 90% confidence interval (CI) (red and blue halos) (Fig 6). This quantitative visualisation reduces complexity and highlights relativity of disease gene expression changes at a chaperome-scale. The polar maps recapitulate characteristic chaperome deregulation signatures in GSA-derived clusters of functional family upregulation and downregulation signatures, for instance in Cluster I cancers such as lung adenocarcinoma (LUAD) (Figs 2 and 6A), or the inverse trend with overall chaperome downregulation in Cluster II cancers such as pheochromocytoma and paraganglioma (PCPG) (Figs 2 and 6B). Concordantly, chaperome polar maps reveal characteristic patterns of cancer groups stratified based on differential expression of ATP-dependent chaperones versus ATP-independent co-chaperones, Group 1 versus Group 2 cancers (Figs 3 and 6). In LUAD, representative of Group 1 cancers, most functional chaperome families, with a preferential enrichment of ATP-dependent chaperones, are upregulated, while sHSPs are reduced (Fig 6A). On the contrary, in Group 2 cancers such as PCPG, gene expression of most chaperome functional families is downregulated (Fig 6B). Inconsistencies between these broad clusters exist, suggesting differences in tissue of origin and molecular underpinnings of respective cancers. However, broad commonalities between distinct cancers originating from the same organ are revealed. For instance, lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) share overall similarity, revealing only subtle differences, for instance in HSP40 expression (Figs 2 and S3). The kidney cancers KICH, KIRP, and KIRC also show similar patterns. As Group 2 cancers, they share and stand out against other cancers with a lack of preferential upregulation of ATP-dependent chaperones (Fig 3), and overall reduced upregulation, or downregulation, of HSP60s (Figs 2,3, 4B and S3). A recent study indeed implicated HSP60 downregulation in tumorigenesis and progression of clear cell renal cell carcinoma (KIRC) by disrupting mitochondrial proteostasis [51]. Overall, these contextual quantitative representations enable an appreciation of the complex chaperome shifts in different cancer tissues derived from > 10,000 patient biopsy samples. The resulting compendium of differential cancer chaperome polar plots (S3 Fig) is also available online through the Proteostasis Profiler (Pro2) tool associated to this study. The integration of disease-related differential transcriptomic changes with the cellular protein interactome network, or the edgotype, is instrumental to our understanding of genotype—phenotype relationships [31]. Towards integrated quantitative views of cancer chaperome deregulation, we curated a high-confidence physical chaperome protein-protein interactome network (CHAP-PPI) to serve as coordinate base grid layout for the analysis of differential chaperome topographies (Fig 1C). We started with 328,244 unique human PPIs (edges) between 16,995 proteins (nodes) downloaded from the BioGRID, IntAct, DIP, and MINT databases [52], [53], [54], [55]. Zooming in on cancer chaperome alterations in the context of physical interactome wiring, we extracted the CHAP-PPI considering the 332 human chaperome genes as previously described [4]. Considering edges with the PSI-MI annotation' physical association' we obtained 272,367 unique physical edges, of which 666 unique edges connect 220 chaperome nodes. We developed a custom script to curate the high-confidence physical CHAP-PPI, considering edges with multiple pieces of evidence, either experimental methods or publications (PMIDs), as more reliable than those supported by only a single piece of evidence. The curation script resolves ambiguous database annotation of methods terms through up-propagation within the PSI-MI ontology tree, only accepting uniquely different or rejecting identical experimental evidence. Automated interactome curation results in eight curation levels (L1—L8), through which we obtain three interactomes of increasing confidence level (see Methods). All 666 unique physical edges between 220 chaperome nodes, without curation for type or number of evidence, represent the single evidence chaperome interactome (SE-CHAP). Curating for high-confidence interactions, we obtained a multiple evidence chaperome (ME-CHAP) comprised of 222 unique physical chaperome edges between 128 chaperome nodes, of which a subset of 132 interactions between 96 nodes is supported by multiple different experimental methods (MM-CHAP) (S2 Table). In order to enable focussed views on transcriptomic alterations of top-level chaperome functional families, we collapsed individual nodes onto functional family meta-nodes, and edges shared between families were collapsed as meta-edges such that meta-node sizes correspond to the number of family members and meta-edge thickness represents the number of shared interactions between families. We considered the meta-interactome derived from the curated high-confidence ME-CHAP interactome (S2 Table), where all meta-nodes corresponding to the 10 functional chaperome families are fully inter-connected in a single network component. We set node colour to visualize cancer gene expression changes (M-scores) and applied a force-directed spring layout algorithm to optimize graph layout [56]. The resulting integrated cancer chaperome meta-interactomes visualize relative chaperome differential changes at reduced complexity across diverse human cancers in the context of physical interactome connectivity (Figs 7A and S4). Next, we extract x-y coordinates of the chaperome meta-nodes in the optimized meta-network graph to serve as a 2-dimensional base grid (x-y coordinates) guiding the spatial layout of 3-dimensional chaperome topographic maps of differential chaperome gene expression changes (M-scores) between cancerous and healthy biopsies (z coordinate) (Figs 7B and S5). This interactome-guided topographic display of differential chaperome alterations enables dimensionality and complexity reduction for the coherent display and comparative analysis of functional network shifts that can serve to compare differential changes i) in disease versus controls, ii) between diseases and disease classes, and iii) between perturbed or unperturbed states across large numbers of heterogeneous genomic datasets. Furthermore, this visualization lends itself for a systems-level assessment of PN deregulation topologies and their readjustment in human disease and therapeutic intervention. We implemented topographic map visualisations into the Proteostasis Profiler (Pro2) suite of tools, to improve accessibility and applicability by the scientific community. Here we exemplify a systematic analysis of differential chaperome gene expression alterations in cancers and neurodegenerative diseases. We reduce complexity through the focus on top-level chaperome functional families. The challenge in this analysis is in the complexity and heterogeneity of available samples for disease groups such as cancers, combined with the multitude of diverse biological processes interconnected within the PN and within its functional processes, as highlighted here at hands of the human chaperome. To date, there has been no systematic interactome-guided analysis of the implications and alterations of cellular proteostasis biology at a systems-level, in a comprehensive set of diseases, such as cancers. Here, we showcase an integrated analytical workflow for the dimension reduction, analysis and visualization of chaperome differential alterations in a representative set of human solid cancers. Our approach focuses on the visualisation of a confined set of Meta-PCA derived quantitative M-scores as descriptors of top-level chaperome functional families. We have developed “Proteostasis Profiler” (Pro2) as an integrated web-based resource and suite of tools, for interactive dimensionality-reduction, analysis and visualisation of disease-specific alterations of proteostasis functional arms, such as the chaperome, in the context of the interactome network. In this study we highlight Pro2 use-cases for the human chaperome across TCGA solid cancers in comparison to the three major neurodegenerative diseases using differential gene expression heat maps (∆GSA) (Figs 2 and 5), Meta-PCA derived quantitative polar plots (M-scores) (Figs 6 and S3), meta-interactomes and interactome-guided 3-D topographic maps (Figs 7, S4 and S5). Pro2 provides an integrated online suite for the application of the underlying algorithms. Pro2 is accessible directly at http: //www. proteostasys. org.
Cancer prevalence, genetic complexity and heterogeneity represent unmet medical need and a significant challenge to personalized medicine, calling for genome-informed therapeutic intervention strategies [57]. While important progress has been made in the elucidation of proteostasis alterations in human diseases, revealing numerous alterations of PN functional processes not only in neurodegenerative or metabolic diseases but also in cancers, paradoxically the characteristics and extent of PN alterations in cancers are largely unexplored and not understood at a systems-level. Cancer cell line global transcriptional characteristics have been extensively studied [58] and numerous individual studies have assessed alterations of various chaperone and co-chaperone expression levels in specific cancers [27,59]. In light of limitations in the clinical translation of hypotheses derived from cell lines and the lack of a systems-level understanding of proteostasis alterations in human disease, we argue that precise quantitative maps of proteostasis deregulation in human disease derived directly from clinical biopsy data will enable precise understanding of the role of PN alterations in pathogenesis towards testable hypotheses and rationalised approaches of PR therapy [1,60]. Here, we focused on the human chaperome, a central PN component, and highly conserved facilitator and safeguard of the healthy folded proteome using an expert-curated human chaperome functional gene ontology comprising an ensemble of 332 chaperone and co-chaperone genes [4] to systematically characterize chaperome alterations in a representative clinically relevant dataset of 22 human solid cancers with matching healthy tissue, corresponding to over 10,000 patient biopsy samples provided through the TCGA consortium [30]. We found the human chaperome to be consistently highly upregulated across the vast majority of cancers assessed. While numerous individual chaperones and co-chaperones have previously been found upregulated in individual cancers [27,59], this knowledge has not been coherently derived from consistent data resources or systematic genome-wide analyses in biopsy tissue before. Here, we provide systematic quantitative maps of chaperome deregulation in cancers that highlight the relevance, characteristics and extent of chaperome upregulation in cancers. Our analysis revealed chaperome deregulation signatures that not only feature broad upregulation of ATP-dependent chaperones but also consistent repression of ER-specific chaperones and the ATP-independent sHSPs. The data also suggest two cancer groups that can be stratified specifically by their chaperome deregulation patterns. Overall chaperome upregulation across cancers is in agreement with existing evidence on individual chaperones that has been previously reviewed [59,61,62]. For instance, elevated heat shock protein expression levels have been reported for HSP90 in breast and lung cancers [63,64], HSP70 was found increased in breast, oral, cervical and renal cancers [65–68], and HSP60 showed increased expression in Hodgkin’s disease [69]. The cellular safeguarding functions of chaperones are subverted during oncogenesis to facilitate malignant transformation in light of increased translational flux and aberrant protein species in cancer cells [27]. Increased chaperone levels have previously been correlated with poor prognosis and cancer survival [59,63,70]. Chronic dependency on stress response and quality control mechanisms drives cancer cells into a phenotype of non-oncogene addiction [28]. The observed extent of chaperome alterations suggests a broader state of cancer “chaperome addiction”, beyond the dependency on individual chaperones. Evidence points towards functional associations between increased proteostasis buffering capacity and maintenance of “stemness”, immortality and proliferative potential in both cancer cells and pluripotent stem cells [27]. For instance, autophagy was found to maintain “stemness” by preventing senescence through sustained proteostasis [71]. Increased proteasomal activity and elevated levels of the HSP60 chaperonin complex TRiC/CCT have recently been linked to stem cell identity by conferring proteostasis robustness [37,38]. Fundamental similarities between stem cells and cancer raise the question to the extent of similarity between cancer and stem cell PN states and capacity. Our data suggest that cancers consistently display signatures of elevated proteostasis functional processes such as the chaperome and proteasome-mediated clearance, and are in agreement with the hypothesis that upregulated clearance mechanisms such as the proteasome and increased chaperome topologies, particularly increases in ATP-driven chaperones such as the HSP60 chaperonin complex TRiC/CCT, confer increased proteostasis capacity and survival benefits to cancer cells just like they are essential to stem cell biology. Precise knowledge of systems-level network deregulation therefore sheds light on fundamental processes at play from stem cell biology to cancerogenesis. Chaperone upregulation is largely regulated through heat shock factor 1 (HSF1) [29]. Overexpression of the TRiC/CCT subunit CCT8 protects against hsf-1 knockdown in C. elegans [38], consistent with a regulatory connection between TRiC/CCT and HSF1 [72]. Connecting processes at the PN level, this evidence suggests a connection between TRiC/CCT and HSF1 stress response signalling also in cancers [38,73]. While increased expression of TRiC/CCT subunits has been observed in cancer cell lines [74], and increases in CCT8 expression are linked to individual cancers [75,76], we describe consistent TRiC/CCT upregulation within global cancer chaperome signatures throughout the majority of TCGA solid cancers, or Group 1 cancers, whereas Group 2 cancers lack chaperome and, to large extent, TRiC/CCT upregulation. Contrary to stem cell proteostasis, which is set up to maintain pluripotency and proliferative capacity, neurodegenerative diseases such as Alzheimer’s (AD), Huntington’s (HD), and Parkinson’s disease (PD) display signs of proteostasis functional collapse. Misfolding diseases feature overexpression of aggregation—prone proteins such as Aβ in AD, α-synuclein in PD, or huntingtin in HD that entail a “toxic-gain-of-function” resulting in chaperome overload, gradually exceeding proteostasis capacity [77], while “loss-of-function” misfolding diseases feature specific perturbations such as dysfunctional ∆F508-CFTR in cystic fibrosis [78]. Functionally deficient steady-state dynamics of the folding environment affect cellular protein repair capacity and proteome maintenance [79]. Most cancer cells however harbour manifold genetic aberrations even at the karyotype level that likely entail dramatic effects on proteome balance [80]. The collective damage caused by oncoprotein expression, compromised DNA repair, genomic instability, reactive oxygen species (ROS), elevated global translation and chaperome overload triggers stress response mechanisms in light of a challenged cellular proteostasis capacity [81]. Chaperome deregulation dynamics observed in cancers indeed display concordantly opposed trends as compared to alterations in the major neurodegenerative diseases. A recent study linked repression of ~30% of the human chaperome in aging brains and in neurodegenerative diseases to proteostasis functional collapse and pointed to the role of a chaperome sub-network as a conserved proteostasis safeguard [4]. Intriguingly, while only ~8% of the human orthologous chaperome had protective phenotypes upon functional perturbation in C. elegans models of amyloid β (Aβ) and polyQ proteotoxicity, chaperones and co-chaperones far less well studied than HSP90 had equally strong protective effects [4]. Similarly, an overlap between the chaperome and the “essentialome” set of 1,658 core fitness genes in K562 leukemia cells [82] found only 55 overlapping with the 332 chaperome genes [83]. Interestingly, HSP60s showed the highest fraction of essential chaperones in agreement with their function as a highly conserved folding complex that hosts ~10% of the proteome’s clients [40]. Collectively, these findings suggest a highly functionally redundant and robust role of the central conserved chaperome within the PN [84]. In summary, our study showcases a systematic profiling of the extent of chaperome deregulation, as a central PN functional arm, in a panel of human cancers and three major neurodegenerative disorders, accompanied by a resource of quantitative multi-dimensional maps with reduced complexity. Therapeutic PN regulation for increased or restored proteostasis capacity may be beneficial in both loss-of-function and gain-of-toxic-function diseases of protein misfolding [2]. Attenuating the PN on the other hand, such as inhibiting chaperones like HSP70 and HSP90 or the UPS clearance machinery, are widely acknowledged as promising therapeutic avenues in cancers [27,83,85,86]. While this manuscript was in preparation, Rodina and co-workers reported findings on a highly integrated chaperome subnetwork, or ‘epichaperome’, as a classifier of cancers with high sensitivity to HSP90 inhibition, while cancers with a less interconnected chaperome are less vulnerable by HSP90 inhibition [26]. Several HSP90 inhibitors have shown encouraging results in clinical trials [87]. Our study further supports the central role of the chaperome in PN biology, justifying particular focus on understanding chaperome alterations in human diseases at a systems level. The characteristic signatures of cancer chaperome alterations revealed in this study suggest broad commonalities and differences that could serve as testable hypotheses for therapeutic chaperome targeting strategies in cancer. Our results underline the value of charting quantitative systems-level maps and provide a resource towards an improved functional understanding of proteostasis biology in health and disease. A systems-level understanding of contextual PN alterations throughout the human diseasome will be instrumental for charting a clearer picture of the PN as a therapeutic target space, and as a resource for clinical biomarkers, including the chaperome. In face of increasing amounts of genome-scale disease data we are confronted with tremendous challenges of data complexity. Therefore, our study provides Proteostasis Profiler (Pro2), an integrated web-based suite of tools enabling processing, analysis and visualisation of proteostasis alterations in human diseases at reduced dimensionality, towards hypotheses-building for mechanistic understanding and clinical translation.
Focussing on pan-cancer analysis of the human chaperome, we chose The Cancer Genome Atlas (TCGA) as the main source for our analyses, as an established dataset that is widely used and adopted by the scientific community. The Broad Institute TCGA GDAC Firehose was accessed to download TCGA RNAseqv2 raw counts data followed by application of the voom method for the transformation of count data to normalized log2-counts per million (logCPM) [88]. Each of these logCPM values were centered gene-wise for sample normalization and comparability and used for all analyses. Considering TCGA clinical data annotation, we extracted those 22 tissue biopsy group datasets that provide both “primary solid tumor” and “solid tissue normal” sample type annotations. We applied Gene Set Analysis (GSA) [33], an advanced derivative of Gene Set Enrichment Analysis (GSEA) [89], in order to assess chaperome gene family expression changes between cancerous and corresponding healthy tissue samples. When applying GSA, we implemented 100 permutations of chaperome genes contained in each functional family in order to allow for statistical assessment of differential expression upon re-standardization of gene groups for more accurate inferences. When applying GSA to the chaperome as one set in comparison to the whole genome (non-chap set), we randomly sampled 332 genes from the whole genome, excluding chaperome genes, and compared them to the 332 chaperome genes in order to exclude bias on group sizes in the comparisons. We applied this random sampling process 100 times in addition to 100 permutations we had on each GSA calculation. We calculated the mean value of all results as a robust measure of chaperome changes with respect to the genome. Results are displayed as heatmaps indicating significance of up or down-regulation of gene expression as ∆GSA values derived from the difference of (1—upregulation p value) — (1—downregulation p value) in disease compared to matching healthy tissue for TCGA cancer datasets, or control patient biopsies for neurodegenerative disease datasets (AD, HD, PD). ∆GSA values are normalized within the interval [-1, +1], where ‘+1’ indicates significant upregulation (upregulation p value = 0), while ‘-1’ indicates significant down-regulation (downregulation p value = 0), accordingly. Bar graphs represent group mean changes of each chaperome functional family gene group over all diseases. We subdivided the human chaperome into functional subsets of chaperones and co-chaperones, and further divided chaperones into two sets of ATP-dependent and ATP-independent chaperones according to the annotations provided by Brehme et al. 2014 [4]. We performed linear modelling using the Limma package in R. Genes with p values < 0. 05 following Benjamini-Hochberg correction are considered in the fraction of differentially expressed genes corresponding to each functional subset. Gene Set Analysis (GSA) is a statistical hypothesis testing method that is by definition limited to confirmatory data analysis with respect to pre-existing hypotheses. In order to serve the goal of quantitative exploratory pan-cancer chaperome analysis, while retaining a maximum information content during model reduction, we devised Meta-PCA, a novel quantitative multi-step dimension reduction model fitting strategy based on principal component analysis (PCA). Principal component analysis (PCA) uses orthogonal transformation to convert a set of variables to linearly uncorrelated variables, such that they are ordered by their information content, which allows for removal of dimensions with lowest information content for dimensionality reduction in complex heterogeneous datasets. In order to stratify cancer from healthy biopsy gene expression samples based on chaperome functional family gene expression in highly convoluted datasets comprising multiple different cancer types, we designed Meta-PCA as a novel two-step method capable of handling this type of heterogeneous data. We hypothesized that each chaperome functional family or process can be described by a low number of variable dimensions, considering that genes within each group are either related or act together in molecular complexes. Therefore, we used a PCA-based approach for quantitative assessment and dimensionality reduction of functional chaperome alterations based on disease gene expression data. Challenged by highly varying sample counts in the different TCGA cancer group datasets, where datasets (tissues) with high sample numbers are at risk of dominating PCA results as compared to cancer groups with low sample numbers, we developed a custom approach that is not limited by a lack of underlying models for interpolation or undesirable loss of information, such as in up- or down-sampling, respectively, allowing us to consider all samples in the included TCGA cancer groups. Assuming distinct roles for each chaperome functional group we define MCHAPx=Fx (Gx) (1) where M denotes the M-score of chaperome (CHAP) family x, Gx is the vector of gene expression values corresponding to genes in CHAP family x, and Fx is the function we want to fit. For simplicity, we considered a linear first degree model as follows: Fx is a vector of weights Wx with identical length as the vector Gx, and we aim to find Wx for all x using PCA. Assuming equivalent biological function of each CHAPx among all tissues, we first calculate Fx for each tissue in order to separate disease from healthy samples for each tissue, and then combine all “relevant” PCs in order to obtain the main underlying PC, or ‘Meta-PC’, of the corresponding CHAP group. We outline the ‘Meta-PCA’ algorithm as follows: Step 0: For each CHAP group and tissue we assume a model MCHAPxt=Fxt (Gxt) (2) Where MCHAPxt is the M-score of CHAP group x in tissue t, Fxt is the unknown function mapping gene expression values for CHAP group x in tissue t to an M-score value, and Gxt is the gene expression vector of all genes in CHAP group x in tissue t. Step 1: Assuming MCHAPxt can be approximated using PC1, we assume Fxt is equal to W tx, which is the vector of weights for CHAP group x and tissue t. Then we calculate PCA on the gene expression matrix (GEX) comprising all genes in CHAP group x, and all samples of tissue t, including ‘solid tissue normal’ and ‘primary solid tumors’. So in this step we have Fxt≃Wxt as loadings of PC1. Step 2: The Fxt≃Wxt assumption in Step 1 is not necessarily true; PCA extracts the most variable direction in GEX, but in case CHAP group x does not change drastically between healthy and cancer, PC1 will represent an unwanted variable or even noise. So we have to filter out the Fxt that did not fit well to the data. For this we use Student’s t-test. For each tissue, we test the separation of MCHAPxt between ‘solid tissue normal’ and ‘primary solid tumor’ samples, and discard all Fxt with p values > 10−4. Step 3: We combine all Wxt to obtain Wx, which is the universal mapping of gene expressions in CHAP group x to its corresponding M-score, regardless of tissue type. Therefore, we calculate Wx as Wx≃M¯xt (3) where the loading of each gene in the universal mapping is the mean value of all the loadings of the same gene on different tissues. Importantly, prior to calculating mean loadings, we set all Wxt to be uni-directed in order preserve directionality of change from healthy to cancer, yielding final Meta-PCs. Wx can be used as the universal function Fx (Eq 1) in order to map a query sample to the corresponding M-score of CHAP group x. Step 3’: In order to validate Fx and resulting M-scores we performed random forest regression using 80% of M-scores and their annotation labels as training set and 20% as test set. In order to visually represent quantifications of chaperome functional family differential cancer gene expression, we used Meta-PCA fitted functions in order to calculate disease-specific M-scores for each chaperome functional gene group as described. We then plotted relevant M-scores using polar plots, such that radial axes represent functional processes. Human physical protein—protein interactions (PPIs), hereafter referred to as ‘edges’, were downloaded on 23 Dec 2016 from the BioGRID [52], IntAct [53], DIP [54], and MINT [55] databases. In order to obtain a high confidence chaperome physical protein—protein interactome network, we developed a custom Python script to curate raw interactome pairs, or edges, as downloaded from the above databases, considering edges detected by multiple experimental methods as more reliable than those detected by only a single method. Similarly, edges supported by multiple publications are considered at higher confidence than edges supported by only one study. Edges supported by multiple methods and / or multiple studies are collectively referred to as ‘multiple evidence’ (ME), of which those identified by multiple different methodologies represent a subset of highest confidence (MM). The Python script processes the interactome raw data as follows: UniProt IDs are mapped to NCBI Entrez Gene IDs and for each human PPI between any two chaperome members (nodes), interacting partners are mapped to Gene IDs. Only edges annotated with PSI-MI term' physical association' type are considered. Eight different curation levels exist: Considering these curation levels, three physical chaperome (CHAP) interactomes of increasing confidence level are obtained (S2 Table): Different PPI source databases may annotate an identical reported PPI to different PSI-MI terms situated at different depth of the same branch within the PSI-MI ontology tree. In these cases, PPIs that are actually only supported by one piece of evidence can unintentionally be mislabelled as multiple evidence PPIs. Our automated quality curation script resolves this problem through up-propagation within the PSI-MI—ontology tree. Assume one PPI is annotated with two different interaction detection methods, A and B, then 1) if PSI-MI ontology tree levels of method A and method B are identical but their PSI-MI terms (IDs) are different, then the methods are considered as different, otherwise A and B are considered the same and the interaction is eliminated from the MM-CHAP interactome, 2) if the level of method A is higher (deeper in the ontology tree) than the level of method B, then the code searches for its parent situated at the same level as method B and compares the parent method ID with B to determine if the methods are identical or different. We considered 2-dimensional physical interactome information to guide the spatial layout (x-y coordinates) of human chaperome functional ontology families in a 3-dimensional (x-y-z coordinates) topographic representation of chaperome M-score changes between disease and healthy tissue (z coordinate). Physical chaperome protein-protein interactome network data (PPIs) was obtained and curated as described above. We considered a network involving only high quality curated interactions supported by multiple pieces of evidence (ME-CHAP). We used the R package iGraph in order to collapse nodes corresponding to each level 1 functional ontology family into meta-nodes, and edges shared between all members of any two different level 1 functional families into meta-edges, such that meta-node size corresponds to the number of family members and meta-edge thickness represents the number of shared interactions between two families. Meta-node colour is set to reflect gene expression changes of each respective functional family in disease. We then applied a force-directed network graph layout algorithm to the meta-network according to Kamada and Kawai [56] and extracted resulting x-y coordinates of each family meta-node in the network. We used Python to draw the meta-network according to the parameters obtained in iGraph to serve as interactome-guided base grid for disease-specific quantitative 3-dimensional topographic network representations. To this end we expanded the 2-dimensional network landscape with Meta-PCA derived chaperome M-score values (z coordinate). We designed a web-based Proteostasis Profiler (Pro2) in order to enable visual exploration of the data and results described in this manuscript, obtained through our algorithms and visualisation tools. Pro2 is accessible directly at http: //www. proteostasys. org. Pro2 is implemented using Django (https: //www. djangoproject. com/) ) ), a web framework written in Python language (https: //www. python. org). All the charts in the tool are generated using the plotly platform (https: //plot. ly). The Pro2 tool itself is hosted on the Heroku platform (https: //www. heroku. com). All R and Python scripts and code related to this manuscript are accessible through the Proteostasis Profiler (Pro2) Github repository at https: //github. com/brehmelab/Pro2.
Protein homeostasis, or proteostasis, is maintained by the proteostasis network (PN), an intricately regulated modular network of interacting processes that evolved to balance the native proteome, supporting cellular and organismal health throughout lifespan. Imbalances and collapse of cellular proteostasis capacity, the capacity to buffer against cytotoxic damage and stress, is increasingly implicated in some of the most challenging diseases of our time, including neurodegeneration and cancers. The systems-level PN alterations in these diseases are not understood to date. Here, we address this challenge, focussing on the human chaperome, the ensemble of chaperones and co-chaperones, which represents a central conserved PN functional arm. We devised a novel data dimensionality reduction approach enabling quantitative contextual visualization of chaperome alterations in the heterogeneous spectrum of cancers based on gene expression data from thousands of patient biopsies. We developed Proteostasis Profiler (Pro2), a new web-tool enabling intuitive visualisation of cancer chaperome deregulation maps. We stratify two cancer groups based on diverging chaperome deregulation and highlight similarities between cancer and stem cell proteostasis. Our study also exposes drastically opposed shifts between cancers and neurodegenerative diseases. Collectively, this study sets the stage for a systematic global analysis of PN alterations across the human diseasome.
medicine and health sciences
biopsy
neurodegenerative diseases
protein interaction networks
topographic maps
surgical and invasive medical procedures
multivariate analysis
mathematics
statistics (mathematics)
network analysis
genome analysis
research and analysis methods
computer and information sciences
geography
genomics
proteins
mathematical and statistical techniques
gene expression
principal component analysis
cartography
proteomics
biochemistry
proteomes
neurology
earth sciences
genetics
biology and life sciences
physical sciences
computational biology
statistical methods
A systematic atlas of chaperome deregulation topologies across the human cancer landscape