In plants, root system architecture is determined by the activity of root apical meristems, which control the root growth rate, and by the formation of lateral roots. In legumes, an additional root lateral organ can develop: the symbiotic nitrogen-fixing nodule. We identified in Medicago truncatula ten allelic mutants showing a compact root architecture phenotype (cra2) independent of any major shoot phenotype, and that consisted of shorter roots, an increased number of lateral roots, and a reduced number of nodules. The CRA2 gene encodes a Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) that primarily negatively regulates lateral root formation and positively regulates symbiotic nodulation. Grafting experiments revealed that CRA2 acts through different pathways to regulate these lateral organs originating from the roots, locally controlling the lateral root development and nodule formation systemically from the shoots. The CRA2 LRR-RLK therefore integrates short- and long-distance regulations to control root system architecture under non-symbiotic and symbiotic conditions.
Plant growth requires the continuous development of the root system and its adaptation to changing environmental soil conditions. Mechanisms controlling root system architecture at the whole-plant level, including the systemic coordination of shoot and root development, are key breeding targets for maintaining crop productivity under adverse stress conditions but remain poorly understood [1]. Root system architecture is a consequence of the sustained activity of root apical meristems, leading to indeterminate root growth as well as the de novo formation of lateral organs. In legume (Fabaceae) plants, the root system can form two types of lateral organs depending on the environmental conditions: lateral roots and symbiotic nitrogen-fixing nodules [2]–[4]. Lateral root initiation, emergence and growth depend on water and nutrient availability and are regulated by a combination of local and systemic pathways [5]. Symbiotic nodules are formed under nitrogen-deprived conditions when the specific Rhizobium spp. soil bacteria are present in the rhizosphere [6]–[7]. In both types of lateral organogeneses, cell divisions are activated in specific tissues (pericycle, endodermis and cortex) above the growing root tip [2]–[4], [8]. Root tissues contributing to primordium formation are, however, different depending on the plants and organs: in the Medicago truncatula model legume, lateral root primordia mainly develop from pericycle cell divisions, whereas the nodule primordia that are induced by Sinorhizobium meliloti mainly derive from the inner cortex. Both types of primordia will then subsequently emerge from the parental root and establish a meristematic stem cell niche ensuring their indeterminate growth. To control meristematic activity, cell differentiation, and lateral organ initiation, non-cell autonomous cues are essential to carry positional information, which can be informed either by mobile phytohormones, small RNAs or peptides [9]–[11]. Among peptides, several CLAVATA 3/EMBRYO-SURROUNDING REGION (like) peptides (so called CLE peptides; [10]) that are perceived by Leucine-Rich Repeats – Receptor-Like Kinases (LRR-RLKs) are involved in local and long-distance (systemic) pathways controlling the development of different plant organs. First, several CLE peptide/LRR-RLK receptor modules carry positional information across a few cell layers to control the cell fate in different Arabidopsis thaliana meristems. The founding example is the CLAVATA3 (CLV3) peptide, which is perceived by the CLV1 receptor to control the shoot apical meristem stem cell niche [12], [13] as well as columella cell differentiation in the Root Apical Meristem (RAM; [14]–[16]). A second example is the Tracheary element Differentiation Inhibitory Factor (TDIF) peptide, which is perceived by the Phloem Intercalated with Xylem (PXY) receptor, controlling stem cell proliferation/differentiation transition in the cambium meristem and therefore vasculature differentiation and organ thickening [17]–[19]. In legume plants, an additional CLE/LRR-RLK module controlling root lateral organs number was identified through grafting experiments as performing a long-distance systemic function from the shoots [7]. Mutants affecting orthologous LRR-RLKs that are closely related to CLV1 in Arabidopsis (SUNN, Super Numeric Nodules in M. truncatula; NARK, Nodule Autoregulation receptor Kinase in soybean; and HAR1, Hypernodulation and Aberrant Root 1 in Lotus japonicus; [20]–[23]) form an increased number of symbiotic nitrogen-fixing nodules depending on the receptor function in the shoots. In addition, the Lotus KLAVIER (KLV) LRR-RLK, which is closely related to the Arabidopsis RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) /TOADSTOOL 2 (TOAD2) that is functionally linked to anther and embryo development, is involved in the same systemic AON pathway as is HAR1 [24]. In M. truncatula and L. japonicus, CLE peptides that are specifically produced in nodulated roots can negatively regulate the nodule number depending on these LRR-RLK receptors [25]–[27]. This CLE peptide/LRR-RLK module therefore participates in the systemic “Autoregulation of Nodulation” (AON; [22]) pathway, which may involve a direct root-to-shoot CLE peptide transport and receptor binding in the shoots, as recently proposed [28]. Interestingly, an increased number of emerged lateral roots was reported in the Lotus har1 mutant under both symbiotic and non-symbiotic conditions [29]. Similarly, KLV also has a non-symbiotic function in the local regulation of SAM maintenance [24]. Collectively, these results indicate that CLE peptide/LRR-RLK signaling modules regulate the development of various organs using either local and/or systemic pathways.
Forward genetic screens that were performed on a M. truncatula Tnt1 insertional mutant collection [30]–[31] identified seven mutant lines with a wild-type shoot development and a “compact root system architecture” (so called “cra”; Fig. 1A–C and S1A Fig.). Segregation analyses of this root phenotype revealed a 3∶1 WT: mutant ratio (chi2 test, p<0. 05, n = 30), suggestive of a single locus recessive mutation. Allelism tests (S1B Fig.) indicated that all of the mutants were affected in the same locus but differed from the previously identified cra1 mutant showing partially similar phenotypes [32] and were therefore named cra2. Detailed quantitative in vitro analyses revealed that the cra2 phenotype consists, compared to the WT, of shorter roots with an increased number of emerged lateral roots (Fig. 1D–E). Accordingly, lateral roots were observed three days post germination (dpg; Fig. 1E) in contrast to WT plants. This root phenotype was observed independently of the growth and nutrient conditions that were used (greenhouse versus in vitro, Fig. 1A, E; with or without nitrogen or carbon sources, Fig. 1E). In addition to the faster emergence of lateral roots, we observed a reduction in the primary root growth, which prompted us to analyze the structure of the RAM (Fig. 2A–C). In contrast to the A. thaliana model, M. truncatula roots have an open meristem, and the transition between cell proliferation and differentiation is more progressive. In addition, cells from diverse files elongate at a slightly different distance from the root apex, leading to a cone-shaped transition zone (Fig. 2A, detail of the transition zone in S2 Fig.). When cra2 RAMs were observed three dpg, both the cell proliferation and elongation zones were reduced, and a lower number of cells was observed in the two zones (Fig. 2 A–C). Root patterning, however, evaluated based on both longitudinal and transversal sections, seemed unaffected (Fig. 2A–B and D). In addition, amyloplast accumulation in differentiated root cap cells and the expression of the RAM stem cell niche marker WOX5 (WUSCHEL-related homeobox 5; [33]–[35]) were both detected in cra2 (S3 Fig.), suggesting the maintenance of RAM cell identity. Among several hypotheses that could explain the cra2 root system architecture phenotype, we tested whether a RAM activity defect could indirectly lead to increased branching as compensation or if the reduced meristematic activity could be a consequence of the enhanced formation of lateral roots. An analysis of root apices at one dpg, i. e. , before any lateral root primordium could be detected in the cra2 mutant, revealed no significant difference in the size of the cell proliferation and elongation zones between WT and mutant roots (Fig. 2E, F). Compared to previous observations of roots at three dpg (Fig. 2A, B), this result indicates that a RAM defect does not precede the occurrence of the lateral root phenotype. As an independent approach, we experimentally removed the RAM at one or three dpg and followed the kinetics of lateral root formation (Fig. 2G, H). The cra2 mutant showed an increased ability to form lateral roots whether RAM excision occurred before or after lateral root initiation. Collectively, these results suggest that the cra2 RAM phenotype can be disconnected from its enhanced ability to form lateral roots. In addition to root branching, legume roots can adapt to environmental conditions by developing another root lateral organ, the nitrogen-fixing symbiotic nodule. An analysis of cra2 roots under symbiotic conditions revealed that a similar “compact root architecture” phenotype was observed (Fig. 3A) and that mutant shoot growth was similar with or without Rhizobium inoculation (Fig. 3B, C). cra2 plants, however, developed a strongly reduced number of symbiotic nodules (Fig. 3 D, E). This low nodulation phenotype could be either linked to a direct CRA2 function in regulating nodulation or may reflect that the strongly altered “compact root architecture” phenotype (e. g. , Fig. 3A) indirectly hampers nodule formation. Using a symbiotic infection kinetic analysis (1 to 14 dpg), we showed that Rhizobium inoculation as early as 1 or 3 dpg led to reduced nodulation (Fig. 3E), indicating that the cra2 nodulation phenotype was independent of the strength of the lateral root phenotype. The few symbiotic nodules that formed on cra2 roots were elongated (S4A Fig.), revealing that a functional nodule meristem was formed. In addition, an analysis of bacterial nitrogenase activity using an Acetylene Reduction Assay (ARA) showed that despite cra2 plants have a strongly reduced ability to fix atmospheric nitrogen due to their lower number of symbiotic organs (S4B Fig.), cra2 nodules have a similar specific ARA activity compared to that of WT nodules (S4C Fig.). Overall, the detailed analysis of the mutant phenotypes indicates that, independently of a potential indirect effect of the increased lateral root formation on nodule formation, CRA2 antagonistically regulates lateral root and nodule formation. As root system architecture is controlled by both systemic and local pathways, we next determined using graftings experiments whether the cra2 phenotypes depend on roots and/or shoots. Under non-symbiotic conditions, the “compact root architecture” phenotype was recovered specifically when the graft combination included cra2 mutant roots (Fig. 4A–B) but was independent of the shoot genotype, indicating that CRA2 expression in roots is sufficient to negatively regulate lateral root formation. Under symbiotic conditions, we surprisingly observed a disconnect between the lateral root and nodulation phenotypes (Fig. 4C–D). Indeed, similar to non-symbiotic conditions, the increased density of the lateral roots was associated with cra2 mutant roots, but the low nodulation phenotype was observed only in grafting combinations that included cra2 mutant shoots and was independent of the root genotype. This result indicates that the systemic activity of CRA2 in the shoots positively regulates symbiotic nodule formation in the roots. Strikingly, the cra2/WT grafts had a WT root system architecture but developed more than 10 times fewer nodules (Fig. 4C–D). In addition, when the nodule numbers were related to the root dry weight to counterbalance the strong differences existing between WT/WT and WT/cra2 root systems, the nodulation efficiency became strictly equivalent between these two grafting combinations (Fig. 4E). These results therefore unambiguously demonstrate that the low-nodulation phenotype is not an indirect consequence of an inhibitory signal that is produced in the numerous lateral roots in cra2. In addition, this result indicates that the root-dependent regulation of the lateral root number is independent of the shoot-regulation of the nodule number. To identify the gene that is affected in cra2 mutants, Tnt1 Flanking Sequence Tags (FSTs) were generated in the different alleles (cra2-1 to cra2-7) to detect Tnt1 insertions affecting a common genomic sequence in several of these mutant lines (cra2-1, cra2-4 to cra2-6; Fig. 5A and S5A–B Fig.). Interestingly, the three other alleles also showed genetic lesions in the same locus, consisting, respectively, in the presence of other M. truncatula endogenous insertional elements for cra2-3 and cra2-7 and in a point mutation for cra2-2 leading to a frameshift (S5C-E Fig.). Based on the FSTs that were available from the Noble foundation (http: //bioinfo4. noble. org/mutant/), we could identify three additional lines with a Tnt1 insertion in the same open reading frame (cra2-8 to cra2-10) that showed a “compact root architecture” phenotype (Fig. 5A and S1A Fig.), further confirming that mutation at this locus is causal for the phenotype. The genomic region that was affected in the 10 available cra2 alleles encodes an LRR-RLK belonging to subfamily XI (Fig. 5B), which, in agreement with the results of the grafting experiment, is expressed in the shoots, roots and nodules (S6 Fig.). Interestingly, this LRR-RLK subfamily contains several other receptors that were identified as regulating plant development via local or systemic regulation [10], [36]. An analysis of CRA2 homology with other LRR-RLKs that have been functionally characterized in legumes showed that CRA2 was not closely related to SUNN/HAR1 or KLV and was most closely related to A. thaliana XIP1 (Xylem Intermixed in Phloem 1; [37]; Fig. 5B). An Arabidopsis xip1 mutant was recently described as showing defects in vasculature patterning specifically in the shoots but not in the roots, and no root developmental phenotype was reported [37]. To determine whether XIP1 and CRA2 may nevertheless have related functions, we analyzed the vascular patterning in cra2 mutant roots and shoots. No significant defect could be detected in the shoot or root cambium structure; in the vascular bundle differentiation, such as the misspecification of xylem and phloem bundles; or in the root or stele diameter (S7 Fig.). These results suggest that XIP1 and CRA2 are not functional homologs. In addition, these results indicate that the cra2 root and nodulation phenotypes (Fig. 1,2) are not linked to any detectable defect in vascular bundle patterning. We then analyzed the CRA2 spatial expression pattern under non-symbiotic and symbiotic conditions using either a transcriptional fusion between an ∼2 kb CRA2 promoter region and the GUS reporter (Fig. 6A–D and I–N) or in situ hybridization (Fig. 6 E–H). Both approaches revealed an expression that was associated with the root stele and vascular bundles (Fig. 6A–B and E–F). Similarly, CRA2 expression was associated with vascular bundles in the shoots (Fig. 6G–H). This result agrees with the expression pattern of other LRR-RLKs regulating lateral root and nodule numbers in different legumes (HAR1/NARK/SUNN or KLV; 24, [38]–[40]). CRA2 was additionally expressed in the Cell Proliferation Zone (CPZ) of the open RAM (Fig. 6A and C–D). In addition, CRA2 was expressed in the lateral root initiation site as soon as the pericycle divisions could be identified (Fig. 6I–J) and later in whole lateral root primordia during the initiation and emergence stages (Fig. 6K–L). Under symbiotic nodulation conditions, CRA2 expression was also detected in nodule primordia (Fig. 6M) as well as in mature nodules in relation to peripheral vascular bundles and the apical meristem (Fig. 6N).
CRA2 functions in the regulation of legume root system architecture suggest that this LRR-RLK acts positively in the Autoregulation of Nodulation (AON) pathway. In the current model, the systemic SUNN-dependent AON pathway represses the number of nodules that are formed on the roots depending on the shoot metabolic status, e. g. , the amount of carbon skeletons that are provided through photosynthesis and that are required for the subsequent assimilation of fixed nitrogen in the nodules. The SUNN pathway therefore limits the formation of extra nodules depending on environmental cues. When initiated, however, a negative feedback mechanism would be necessary to reset this negative regulation and further permit new symbiotic infection events. The CRA2 LRR-RLK may then participate in the systemic dynamic fine tuning of nodule formation. As both CRA2 and SUNN LRR-RLKs are expressed in the shoot vasculature, it remains to be determined whether and how these proteins act together or independently. As LRR-RLKs have been proposed to form large complexes comprising different members of the family [36] or even other RLKs (e. g. , CLV1 with ACR4 in the RAM; [16]), CRA2 and SUNN may interact within a single complex. The Lotus HAR1-dependent AON pathway was additionally shown to control negatively lateral root formation [20]–[29]. In the sunn Medicago mutant, however, the root length is reduced, but no specific function in the regulation of lateral root number has been identified [23]. CRA2 therefore negatively affects lateral roots similarly to HAR1 in Lotus but dissimilarly to SUNN in M. truncatula. Interestingly, a shorter root length and a decreased number of lateral roots occurred in the Lotus klv mutant, whereas additive effects of klv and har1 were identified for nodulation, indicating their related function in a single AON pathway [40]. In addition, both har1 and klv antagonistic lateral root phenotypes were observed under non-symbiotic conditions [29], [40], similar to the phenotype of CRA2 in M. truncatula. This result indicates that these peptide/LRR-RLK regulatory modules acting under non-symbiotic conditions may also control lateral root formation in non-nodulating (non-legume) plants. In Arabidopsis, the ACR4 CRINKLY-RLK regulates lateral root initiation [41], but no LRR-RLK has been yet associated with this developmental process. More generally, despite the close relationships between HAR1, SUNN and CLV1, their mutant phenotypes are not conserved between plant species: the sunn and har1 mutants have divergent lateral root phenotypes, whereas no shoot fasciation phenotype was detected in the har1 or sunn mutant in contrast to clv1 in Arabidopsis. Similarly, despite CRA2 and XIP1 having closely related sequences, no vasculature patterning phenotype was identified in cra2 compared to xip1, and no altered root system architecture phenotype was reported in xip1. This result suggests that various sets of LRR-RLKs differentially regulate the ability of root systems to form lateral roots depending on species, even inside the legume family. Alternatively and non-exclusively, different patterns of LRR-RLK gene duplication may have occurred in the different plant genomes, generating functionally divergent or redundant pathways. These scenarios may explain the apparent phenotypic diversification that is observed. Overall, this study demonstrates that a single LRR-RLK acts locally in roots and systemically in shoots to control root lateral organ development, thereby coordinating at the whole-plant level the plastic development of the root system depending on the changing environmental conditions. To elucidate the opposite effects of the CRA2 pathway on lateral root and nodule formation, the identification of downstream targets will be essential. Among other candidate pathways, cytokinins were previously reported to antagonistically control lateral root and symbiotic nodule formation [42]. Data on crosstalk between signaling peptides and hormones is just emerging, mainly with auxin and cytokinins [10]. Therefore, a remaining challenge will be to integrate the different peptide/receptor modules that are known to regulate lateral root and nodule formation, including the CRA2 pathway, into the framework of hormonal regulation.
The Medicago truncatula (Gaertn.) plants used in this study were of the R108-4 genotype. The Tnt1 insertional mutants were generated and screened at the Noble Foundation (USA; lines named NFxxx; [30]) or produced at the “Institut des Sciences du Végétal” (ISV, CNRS, Gif sur Yvette, France) and screened at the “Agroécologie” institute (INRA, Dijon, France; lines with other names than NFxxx). The seeds were scarified on sandpaper, sterilized for 20 min in bleach (12% [v/v] sodium hypochlorite), and thoroughly rinsed in sterile water. The seeds were then stratified at 4°C for one day and then germinated at 25°C in the dark on inverted water agar plates. The seedlings were grown in vitro in a growth chamber at 25°C with a 16 h light period and a 150 µE intensity on N-deprived media (“i”, [42]; or Fahraeus, [43]), on an N-rich medium (Fahraeus with NH4NO3 10 mM), or on a N- and C-rich medium (“Lateral-Root-Inducing Medium”, LRIM; [42]) depending on the experiment. Alternatively, the plants were grown in a greenhouse (25°C, 16 h 150 µE light period, 60%–70% humidity) in soil or in pots containing a perlite∶sand mixture (3∶1) and watered every two days with “i” or “SN/2” media [42], depending on the experiment. The nodulation experiments used the Sinorhizobium meliloti 1021 strain (OD600nm = 0. 05) as described in [42]. Plant genomic DNA was extracted from the leaves as described by [44], and Flanking Sequence Tags (FSTs) that were linked to Tnt1 insertions were identified using the transposon display PCR method as described in [45] using the EcoR1 or Ase1 restriction enzymes. The transposon display, genotyping PCRs, and the sequencing of the different mutant alleles were performed using the primers that are listed in S1 Table. The RT-PCRs and real-time RT-PCRs were performed as described in [42] with the primers that are indicated in S1 Table. To generate the MtCRA2 reporter transcriptional fusion, an 1800-bp sequence upstream of the CRA2 start codon was identified, corresponding to the upstream region of the Medtr3g110840. 1 open reading frame (M. truncatula genome Mt4. 0v1, http: //www. jcvi. org/medicago/index. php). This region was amplified by PCR using a high-fidelity polymerase (Phusion, Thermo Scientific) and primers pCRA2-F and pCRA2-R (S1 Table). The promoter was cloned using Gateway technology initially into the pTOPO-Entry vector (Invitrogen) and then into the pkGWFS7 vector (https: //gateway. psb. ugent. be/search) carrying a Green Fluorescent Protein (GFP) - β Glucuronidase (GUS) fusion downstream of the cloning recombination site. The graftings were performed as described in the “cuttings and grafts” chapter of the Medicago handbook (http: //www. noble. org/medicagohandbook/). The grafts, which were initially generated in vitro, were transferred after three weeks to pots containing a perlite∶sand mixture (3∶1) that was imbibed with the “i” solution in a growth chamber (same conditions as described above). After three weeks, the root length and root dry weight (60°C for 48 h) were measured. For root apex excision, the roots were sectioned at one centimeter from the root tips one or three days post germination and placed in the “i” medium in a growth chamber (same conditions as described above). The number of lateral roots was quantified one to seven days post root tip excision using the ImageJ software (http: //imagej. nih. gov/ij/). To generate composite plants, constructs were introduced into the Agrobacterium rhizogenes ARqua1 strain and used for M. truncatula root transformation as described in [46]. Transgenic roots were selected on kanamycin (25 mg/L) for two weeks, and composite plants were then transferred onto growth papers (Mega International, http: //www. mega-international. com/) on “i” medium for four to six days and, depending on the experiment, were inoculated by S. meliloti as described in [42]. The roots or stems were cut into 5-mm segments and immediately embedded in 3% agarose (Euromedex). A vibratome (VT1200S, Leica) was used to generate 100 µm-thick cross-sections. Toluidine blue and aniline blue (Sigma) stainings were performed by incubating sections for 3 to 5 min in 1% toluidine blue or in 0. 005% aniline blue and subsequently washing the sections twice with distilled water. The sections were viewed under bright-field illumination or under UV excitation (365 nm), respectively, for toluidine blue or for aniline blue stainings using a Leica DMI 6000B inverted microscope. The phloroglucinol–HCl reagent was prepared by mixing 2 volumes of 2% (w/v) phloroglucinol (Sigma) in 95% ethanol with one volume of concentrated HCl and observation under bright-field illumination (DMI 6000B, Leica). Callose fluorescence was visualized using a 405 nm excitation, and emission was collected between 480 and 515 nm (DMI 6000B, Leica). The amyloplasts were detected using Lugol (Sigma) staining and visualization under bright field illumination. To examine the detailed cellular organization of M. truncatula root apices, the roots were stained using a modified Pseudo Schiff-Propidium Iodide (PS-PI) staining protocol as described by [47]. Longitudinal optical sections were obtained using a Leica TCS SP2 confocal laser-scanning microscope using a 488 nm excitation, and emission was collected between 520 and 720 nm. The root meristem size was estimated as the number of cells in the outer cortex from the location of the tip to the first elongating cell using the ImageJ software. The length of the elongated cortical cells in the mature root region was also measured. The GUS activity was revealed as described by (48] and observed under bright-field illumination (DMI 6000B, Leica). In addition, using the AOBS reflection mode of a Leica TCS SP8 spectral confocal laser-scanning microscope, the GUS precipitate reflectance was analyzed as described in [47] with a 488 nm excitation and a collection of the reflection signal between 485 and 491 nm on PS-PI counterstained samples (detected as described above). In situ hybridization was performed as described in [48] with the probe as indicated in S1 Table. To measure nitrogenase activity of symbiotic nodules, an Acetylene Reduction Assay (ARA) was performed on individual plants with a protocol that was derived from [49]. One month after inoculation with Rhizobium, the plants were placed into 10-ml glass vials that were sealed with rubber septa. Acetylene was injected into each vial, and after 1 h of incubation at room temperature, the produced ethylene was measured using Gas Chromatography (7820A, Agilent technologies). Phylogenetic analyses were performed using the SeaView package (v. 4. 4. 0; [50]). The full-length protein sequences that were retrieved from the NCBI database were aligned using Muscle and optimized with the Gblocks software. Phylogenetic relationships were defined using a maximum likelihood approach. The tree was built with the PhyML software using the LG substitution model and four substitution rate categories. Support for each node was gained by approximate likelihood ratio tests (aLRT SH-like; [51]). The XP_001698687. 1 RLK from Chlamydomonas patens, which was identified by a BLAST search of NCBI (E-value = 1e-40), was used as an outgroup to anchor the tree. The statistical analyses were performed with non-parametric tests (Mann-Whitney when n = 2 independent samples and Kruskal and Wallis when n>2 independent samples).
Despite the essential functions of roots in plant access to water and nutrients, root system architecture has not been directly considered for crop breeding improvement, but it is now considered key for a “second green revolution. ” In this study, we aimed to decipher integrated molecular mechanisms coordinating lateral organ development in legume roots: lateral roots and nitrogen-fixing symbiotic nodules. The compact root architecture 2 (cra2) mutant form an increased number of lateral roots and a reduced number of symbiotic nitrogen-fixing nodules. This mutant is affected in a CLAVATA1-like Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) that has not previously been linked to root development. Grafting experiments showed that CRA2 negatively controls lateral root formation and positively controls nodule development through local and systemic pathways, respectively. Overall, our results can be integrated in the framework of regulatory pathways controlling the symbiotic nodule number, the so-called “Autoregulation of Nodulation” (AON), involving another LRR-RLK that also acts systemically from the shoots, SUNN (Super Numeric Nodules). A coordinated function of the CRA2 and SUNN LRR-RLKs may thereby permit the dynamic fine tuning of the nodule number depending on the environmental conditions.
developmental biology
plant growth and development
symbiosis
genetics
plant genetics
molecular genetics
biology and life sciences
species interactions
molecular biology
Local and Systemic Regulation of Plant Root System Architecture and Symbiotic Nodulation by a Receptor-Like Kinase