The intestinal microbiota is a microbial ecosystem of crucial importance to human health. Understanding how the microbiota confers resistance against enteric pathogens and how antibiotics disrupt that resistance is key to the prevention and cure of intestinal infections. We present a novel method to infer microbial community ecology directly from time-resolved metagenomics. This method extends generalized Lotka–Volterra dynamics to account for external perturbations. Data from recent experiments on antibiotic-mediated Clostridium difficile infection is analyzed to quantify microbial interactions, commensal-pathogen interactions, and the effect of the antibiotic on the community. Stability analysis reveals that the microbiota is intrinsically stable, explaining how antibiotic perturbations and C. difficile inoculation can produce catastrophic shifts that persist even after removal of the perturbations. Importantly, the analysis suggests a subnetwork of bacterial groups implicated in protection against C. difficile. Due to its generality, our method can be applied to any high-resolution ecological time-series data to infer community structure and response to external stimuli.
The intestinal microbiota has been receiving much attention lately. Recent studies, propelled by metagenomics and next-generation DNA sequencing technologies, establish novel connections between the intestinal microbial species composition and diseases [1]–[3]. An imbalance in bacterial composition has been linked to chronic diseases such as obesity [4], Crohn' s disease [5] and type 2 diabetes [6]. Even drug-induced transient changes in the microbial community can increase the risk of developing diseases such as acute intestinal infections [7], or pulmonary viral infections [8] in mammalian hosts. Although its importance has long been acknowledged [9]–[12] studies of the microbiota had been limited by the fact that most microbes are uncultivable in the lab. The recent developments in metagenomic high-throughput sequencing allow this by enabling the investigation of species composition directly without the need for culturing [13]. This has opened a new window into the microbial ecosystem residing in the intestinal tract. Our present view is that the intestinal microbiota is a relatively resilient ecosystem [14], with a composition that is quite stable over time [15], [16]. External perturbations, such as dramatic changes in diet [17] or antibiotic administration [18], can shift the composition. For example, broad-spectrum antibiotics can remove highly abundant species and allow less abundant, antibiotic-tolerant bacteria to dominate [7]. Antibiotic-induced losses of biodiversity increase the risk of bacterial infections [19], [20] and the effects can persist for several days after antibiotic treatment [18], [19], [21]. Perturbation-induced composition shifts are often observed in multispecies microbial ecosystems and may affect macroscopic overall functionality [22]. The loss of protective species can be resolved by reintroducing normally resident (or commensal) microbes. Faecal transplantation, i. e. the reestablishment of a patient' s intestinal microbiota by introducing the microbiota of a healthy donor, is highly effective against Clostridium difficile induced colitis [23], [24]. Similarly, the reintroduction of anaerobic flora with high levels of Barnesiella sp. can clear intestines from highly abundant vancomycin-resistant Enteroccocus in mice [25]. In order to understand how commensal consortia confer resistance against pathogens it is crucial to identify the network of interactions between the species [26]. Interactions can be mediated by a direct secretion of substances such as bacteriocins [27], or ecological competition between the microbes [28], or even indirect interactions through immune system modulation [29]. Most quantitative studies of the intestinal microbiota so far focused on comparing the composition of different samples using quantitative indices and correspondence analyses [14] and cross-sectional statistical tests [1], [30]. Likewise, associations between microbial species are often obtained using correlation-based algorithms [26], [31]–[36], which results in undirected interaction networks. Singular value decomposition [28] or mixture model engines [37] allow for individuating stereotypical modes of response to external perturbations (i. e. grouping species positively or negatively affected by the stimulus) but they provide no information on the interactions themselves (Figure 1A). We recently introduced an ecological model of microbiota dynamics that considers both species interaction networks and extrinsic perturbations such as antibiotics [28]. The model can explain how relatively simple ecological interactions such as competition for nutrients can lead to complex phenomena as, for example, multi-stability or antibiotic-mediated catastrophic shifts. Importantly, we concluded that quantitative knowledge of the microbial interactions could enable the prediction of microbiota dynamics. Predictive models can be of great therapeutic value by guiding antibiotic selection to reduce the risk of antibiotic-induced enteric disease [20]. However, no study to date has generated predictive models of ecological interactions and antibiotic perturbations. Inspired by work on interaction inference in cheese-associated microbial communities [38] we extend the generalized Lotka–Volterra equations [39], [40] to infer microbiota ecology and predict its temporal dynamics under time-dependent external perturbations. A related approach based on linear ordinary differential equations has already been applied to gene-interaction networks [41]–[44]. Specifically, our method enables the quantification of (1) growth rates of microbial species, (2) species–species interactions, and (3) susceptibilities of microbial groups to time-variable external perturbations such as antibiotics. Moreover, we can use these parameters to numerically predict dynamics of the microbiota and to characterize its stability (Figure 1B). Using this method, we analyze data from a recent mouse study [19], which shows that the antibiotic clindamycin increases susceptibility to Clostridium difficile colonization. Our results suggest the existence of resilience and multistability in the intestinal microbiota and lead to a hypothesis on a subnetwork of microbial groups involved in the native resistance against pathogen colonization. This study demonstrates that data-derived models of microbiota dynamics can have significant analytic and predictive power. As such, inference and prediction algorithms could be used in combination with metagenomics to assist in the rational design of therapies such as antibiotic or probiotic therapies [12].
Extracting model parameters using a time-discrete Lotka–Volterra system has already been presented in the context microbial communities [38], [45], [46]. We extend this approach by introducing time-variable perturbations and applying Tikhonov regularization to solve the discretized Lotka–Volterra equations. Furthermore, we use the obtained parameters to predict dynamics and assess the system' s stability. In this spirit, we adopt the general deterministic approach of modeling time-dependent ecological dynamics using generalized Lotka–Volterra equations [39] with the addition of external perturbations. Formally, this model consists of autonomous, non-linear, coupled first-order ordinary differential equations, (1) Here is the concentration of a focal species, , at time, is its specific growth rate, is the effect of the interaction of species on species and is the susceptibility to the time-dependent perturbation (for instance, an antibiotic or diet). Ecological time-series data, such as longitudinal metagenomic sequencing data [15], [47], provide the composition of a community at discrete time points. Temporally resolved metadata, such as the timing of antibiotic administration [20] or of changes in diet regimes [17], may also be available and provide information about processes that perturb the microbiota. In order to translate the time-discrete data to a time-continuous dynamical system we divide (1) by and discretize (see Materials and Methods), (2) The model parameters are determined by a linear system of equations, which is then solved using Tikhonov regularization [48] in order to ensure uniqueness and stability of the solution, (3) The values for the regularization parameters, , can for example be found in -fold cross-validation (we use) as the minimizer of the mean-squared stepwise prediction error to set the optimal trade-off between data fit and robustness towards the introduction of unseen or missing data [49]. The inference method was first tested on in silico data by generating trajectories for a Lotka–Volterra model as defined in (1). We created multiple trajectories of ecological systems characterized by different population sizes, random growth rates, interaction values and susceptibility parameters while ensuring system stability [50], 51. The simulations were also subjected to random perturbations of variable duration and white noise was added to simulate measurement uncertainty (Figure S1). The test confirms that the minimum of the stepwise prediction error can be used as a suitable proxy for the minimization of the parameter inference errors (Figure S2). Given the inferred parameters we can now predict the temporal dynamics by solving (1). We applied this approach to in silico data. The results are presented in Figure S3. In a recent study, Buffie et al. described experiments on the effect of the antibiotic clindamycin on the intestinal colonization with the spore-forming pathogen C. difficile [19]. The experiments were performed in a mouse model and high-throughput DNA sequencing was used to measure the relative abundance of bacterial species in cecum and ileum. The experiment consisted of three distinct populations of mice. The first population received spores of C. difficile, and was used to determine the susceptibility of the native microbiota to invasion by the pathogen. The second population received a single dose of clindamycin to assess the effect of the antibiotic alone. Finally, the third population received a single dose of clindamycin and, on the following day, was inoculated with C. difficile spores. The untreated mice challenged with C. difficile (population #1) did not develop infection and maintained a stable microbiota throughout the entire experiment. The single dose of antibiotic (population #2) resulted in a dramatic reduction in the microbiota biodiversity, with more than 90% of the initial species dropping below detection. The effects of this perturbation were long lasting, and biodiversity did not return to pre-treatment levels even 28 days after the clindamycin dose. Finally, mice that received C. difficile following the treatment with clindamycin (population #3) were colonized by the pathogen, with 40% of those mice dying due to C. difficile induced colitis. The experiment was performed in three replicates: for each population three mouse colonies were uniformly treated, but separately housed. Each time point represents a mouse from each colony which was sacrificed to determine the intestinal microbiota composition. Mice from the same colony are biological replicates which justifies the interpretation of these compositions as one time line representing one co-housed mouse population [19]. We used the cecal content data to infer microbial interactions, growth rates and susceptibilities to clindamycin (see Materials and Methods). Our mechanistically-based model presupposes absolute abundances. Therefore, we converted the normalized DNA sequence abundances obtained by metagenomics by multiplying with the number of universal 16S rRNA per gram of cecal content (measured using qPCR) multiplied by the sample density, [52] (the actual density value has little importance for the inference of the interactions given the model scaling invariance, see Materials and Methods). For consistency with the previous study [19] we integrated only the ten most abundant genera including the pathogen C. difficile, together accounting for the vast majority (approx. 90%) of the total sequences obtained from 16S rRNA high-throughput DNA sequencing (Figure S4). The remaining lower abundance microbes were grouped into a category called “Other” (see Materials and Methods). This choice resulted in less than 30% of undetected entries in the data matrix. The choice of a higher number of independently treated genera, e. g. 15, could result in more than 50% of missing values in the data matrix (Figure S5). Consistent with the underlying biological assumptions, the specific growth rates obtained from our inference method (Figure 2A) are all positive, and concordant with values measured in vitro using representative species of human colonic microbiota (0. 55–1. 78 per day [53] compared to 0. 2–0. 9 from Figure 2A). The diagonal elements of the obtained interaction matrix (Figure 2B) are negative. This is again consistent with the underlying biology, since it means that each of these species would eventually reach carrying capacity even in the absence of other species. Coprobacillus is found to be the genus with strongest interactions by value in the ecological network. Specifically, it appears to primarily inhibit all the other microbes, including C. difficile, with the exception of Akkermansia and Blautia which also show inhibitory effect on C. difficile. The strongest inhibitory effect is on Enterococcus which together with the group of unclassified Mollicutes is inferred to positively interact with the pathogen C. difficile. This positive association is consistent with previous reports [54], [55]. Intriguingly, our method also suggests Barnesiella to mildly inhibit Enterococcus, which agrees with previous findings in mice and humans [25]. Susceptibilities to clindamycin (Figure 2C) propose that the antibiotic inhibits all of the genera, except for Enterococcus and the group of undefined Enterobacteriaceae. C. difficile itself is mildly repressed by the antibiotic. Next, we investigated the implications of the inferred model parameters for microbiota dynamics. First, we tested the model' s performance in predicting microbiota trajectories. To do so, we inferred the growth, interaction and susceptibility parameters on of the available data, leaving of the trajectories to test the model prediction. Subsequently, we solved eq. (1) numerically using the inferred parameters, initial compositions and the metadata of antibiotic and/or C. difficile inoculation (see Materials and Methods for further details). In Figure 3, we compare the observed dynamics of the second replicates with the dynamics inferred from the first and third replicate. Figure S6 shows the full comparison for all the three replicates. The simulated trajectories show a good agreement with the experimental data for all the three populations with respect to order of magnitude and qualitative behavior. There are, however, discrepancies especially in Figure 3B. Here, the experimental data shows a community take-over of Akkermansia and Blautia three days after clindamycin treatment. Our method predicts the same behavior but with several days delay (see Discussion for possible explications and model limitations). The rank correlation between data and prediction is of 62% along time (Figure 3D). We then investigated the long-term stability of the system. We calculated the steady-state composition of the microbiota, , as a solution of eq. (1) for vanishing the time-derivatives in the absence of any perturbation. Consequently, there are steady states where is the number of microbial groups in the system. Of these, one state corresponds to the trivial case of total extinction (), one state corresponds to the case of total coexistence (, for invertible), and states correspond to the permutations of existence or extinction for every other species [56]. A priori, we have no knowledge about which one of these states the system will attain. This depends on the initial composition, presence and duration of the external perturbations. Therefore, we determine the steady state by simulating long-term dynamics to obtain information on species extinction and coexistence. Once this information is obtained, we can analytically evaluate the steady state of the system and its qualitative behavior by determining the spectrum of the corresponding Jacobian matrix evaluated in that state (see Materials and Methods). The principle of linearized stability states that if the real part of the largest eigenvalue of the Jacobian is negative then the composition represents a stable microbiota (an asymptotically stable state). Otherwise, it is unstable [57]. For instance, the total extinction state, , is unstable if any of the growth rates is positive, which is true for our data (Figure 2A). However, the dynamics of high-dimensional Lotka–Volterra systems allow for a large variety of different qualitative behaviors such as limit cycles, chaos or attractors [39]. We applied this analysis to our system and identified one unique steady state for each independent replicate (Figure 4A). The replicate corresponding to untreated mice challenged with C. difficile (population #1) is characterized by high abundance of clindamycin-sensitive bacteria such as Barnesiella, undefined Lachnospiraceae and unclassified Lachnospiraceae. The steady state corresponding to clindamycin application (population #2) is characterized by a take-over by Blautia, unclassified Enterobacteriaceae and unclassified Mollicutes. Finally, for the case corresponding to C. difficile after clindamycin (population #3), the steady state predicts severe C. difficile colonization in addition to the genera emerging in population #2. Interestingly, these steady states agree in order of magnitude, community profiles and composition with the last experimentally measured data point of Figure 3A–C. However, in the observed trajectories the composition still changes between the last two observed data points. This could be due to the fact that the microbiota is not yet stabilized (i. e. still in transient dynamics) or due to the effect of fluctuations [15]. Although this cannot be discerned from a simple observation of the data, assuming that our model captures the actual microbiota ecology our analysis suggests that the microbiota of the perturbed microbial communities did not recover their original composition within 28 days from treatment cessation. Rather, the microbiota stays in distinct, perturbation-history dependent equilibria. The intact microbiota is, by antibiotic administration, driven towards a composition which is more susceptible to C. difficile colonization. By subsequent introduction of the pathogen, the community is dragged into an alternative stable composition including the otherwise repelled C. difficile; this may be an example of “niche opportunity” [58], [59]. Interestingly, when considering the landscape of all possible steady states of the inferred Lotka–Volterra model, unstable steady states, i. e. those referring to critical compositions which drive communities with similar compositions to a collapse or catastrophic shift [60], are significantly more often observed than stable ones. Given the inferred parameters, we find that of the steady states which the system is able to attain from a composition of L initially present genera, about 98% are found to be unstable (Figure 4B). Nonetheless, our model predicts the existence of multiple stable compositions in each of the three experimental arms. Our results, therefore, may indicate the existence of alternative stable compositions of the intestinal microbiota; switches between these states are induced by perturbation with clindamycin or C. difficile inoculation. This concept is reminiscent of ecological stability and resilience discussed by Connell and Sousa [61]. The inspection of the model inferred from mouse experiments [19] could suggest a possible ecological mechanism for C. difficile colonization (Figure 5A). In the intact microbiota, our method proposes that Coprobacillus interacts positively with the genera of Akkermansia and Blautia. Additionally, Coprobacillus inhibits Enterococcus, which, when increasing in abundance, enhances C. difficile establishment. Without clindamycin, the three genera Coprobacillus, Akkermansia and Blautia, maintain intestinal stability and confer resistance against C. difficile colonization (Figure 5B). However, when clindamycin is administered, Coprobacillus, Akkermansia and Blautia, are inhibited while Enterococcus is promoted. As the three protective groups decrease in abundance, our results suggest that Enterococcus increases in abundance and may facilitate colonization by C. difficile. We discuss the validity of this mechanism in the Discussion section.
We presented a general method for the inference and prediction of multispecies ecological community dynamics under perturbations. Although this method was primarily developed having in mind the intestinal microbiota, the same method may be potentially applied to time-resolved data from any ecological systems, such as bioreactors [62], marine [63] or soil ecosystems [64]. Our method quantifies growth rates, community interactions and susceptibilities to external perturbations in a single inference. The modeling approach is based on the generalized Lotka–Volterra model (eq. (1) ), a system of non-linear ordinary differential equations, whose governing parameters can be stably determined by a regularized regression on the discretized version of the model (eq. (2) ). Microbiota metagenomics data often have a high number of microbial species which is much larger than the number of available time points. This presents a challenge to inference. We solved this problem in two steps. The first step was to group the bacterial sequences at the genus level of phylogenic classification and consider only the ten most abundant microbial genera including the pathogen C. difficile and merge all remainders to “Others”. The second step was to apply Tikhonov regularization, a procedure that provides a unique and stable solution and, in combination with cross-validation, reduces the risk of overfitting noisy data. Our inference method was tested using in silico data (Figure S1) and evaluated by its ability to recover left-out data using a cross-validation approach (Figures S2, S3). The application of inference methods to temporal metagenomic data shows great promise. Still, the development of accurate, predictive models, for example for clinical application, will require further developments and the next few years are sure to see major improvements in this area. For example, the method used here to group microbial sequences may be expanded by adding functional information in addition to taxonomic information. Future methods will benefit from deeper sequencing of the metagenome [65] to inform new ways to define functional microbial groups. Such analyses can shed new light, for example, on the mechanisms by which the abundance of certain species seem to correlate with susceptibility to colonization by closely related pathogenic bacteria [66]. Regarding antibiotic effects, even though we are not yet able to measure the effective concentrations of the antibiotic in the intestine in a high-throughput manner, more accurate information on the pharmacokinetics in vivo will greatly enhance the applicability of this method to clinical settings. Likewise, experimental advancements with animal models will also be crucial. The experiments analyzed here consisted of a single dose of clindamycin of by intraperitoneal injection [19]. Comparing antibiotic perturbed mice with intact mice in this case is similar to comparing a thriving forest with one that has burnt to the ground. The same antibiotic administered in gradual dosages, or the use of other antibiotics, will surely produce distinct effects and would allow for analyzing the communities with distinct compositions. Also, engineered artificial microbiota with defined numbers of bacteria in germ-free mice could be a valuable tool to test the resilience of communities with increasing complexity. Longitudinal data collected from such experimental models can give valuable new insight into the mechanisms of protection against C. difficile. Other differences between data and simulation results may stem from approximating the infinitesimal by time-discrete dynamics and the fact that the Lotka–Volterra model incorporates only pairwise, second-order interactions (eq. (1) ). This could be relaxed in the future by extending the model to third or higher-order interactions once more data becomes available. Furthermore, due to the requirements of the Lotka–Volterra framework our method cannot be applied directly to read count data without additional information on the total number of bacteria per volume unit. If this information is not available it needs to be estimated which can be a source of deviations between measured and predicted results. Nevertheless and even though we cannot claim that the inferred interactions are revealing real causative relationships among microbes, we believe that our results go beyond the explanatory power of widely-used correlations and other methods used. A major advantage of this method is its foundation on a mechanistic framework. This allows for the determination of directional interactions as well as the simulation of microbial dynamics with considerable agreement with the actual data. Based on our inference results, we also hypothesized on a mechanism of C. difficile colonization. However, making a substantiated statement on this mechanism would require further analysis across different host systems and under various antibiotic perturbations. Moreover, due to the limited phylogenetic resolution of the 16S rRNA sequencing, our approach would assign the effects of possibly few, interaction-mediating strains to the whole genus. Nevertheless, the analysis presented here suggests possible experiments focusing on the role of Enterococcus, Coprobacillus, Blautia and Akkermansia in mediating C. difficile colonization. This could be investigated, for example, in mice with engineered microbial consortia. Specifically, the microbiota of these mice could be manipulated to lack the genus of Enterococcus or to contain after antibiotic treatment representative strains of genera such as Coprobacillus, Blautia and Akkermansia which are predicted to have protective effect. Non-colonization and clearance of C. difficile in this system after clindamycin application would then support our hypothesized infection mechanism. There is an urgent need to understand how the commensal intestinal microbial community resists invasion by pathogenic species. Mathematical modeling and inference can help shed new light on this problem by disentangling the contribution of each factor at play. The combination of increasingly accurate and affordable sequencing methods with solidly grounded mathematical theory can help advance our understanding of the relationship between the human host and its microbial inhabitants.
A general approach for a deterministic model of time-dependent ecological dynamics is given by the following system of autonomous coupled first-order ordinary differential equations, in which each time course represents the time-variation in abundance, , of an ecological unit in a certain environment, (4) with unknown parameters, for. A requirement of ecological models for closed systems is that a unit that once goes extinct cannot return. Thus, for unit which is extinct at time, we require and at any time independent of any variation of the remaining, . In the framework of (4), this necessitates, for and for such that, if we restrict to only pairwise interactions, we obtain for each unit, (5) where and for. This system of equations is also known as the Lotka–Volterra model [39]. The denotes the unlimited growth rate of unit in absence of any competition. The interaction term characterizes the effect of unit on. In particular, stands for activation and for repression. (No interaction is accordingly indicated by). In this form, the model, which is governed by the absolute abundances of units and their physical, order-dependent interactions, also captures non-linear dynamics such as Monod-type/Michaelis–Menten kinetics in a first-order approximation. In addition to growth and interactions we introduce the effect of the application of external time-dependent stimuli, , on each ecological unit such that the full model writes, (6) where represents an external, time-variable stimulus of a perturbation whose relative susceptibility for each unit is represented by. In the framework of metagenomic data, one faces large magnitudes of total numbers of bacteria. A common approach to identify scale-dependencies of the system and to circumvent numerical problems associated with this is to use non-dimensional variables which allow to treat the model relative to changes on typical system scales [67]. For this purpose, we introduce the following representation of the dynamical variables, (7) where the dimensionless forms are denoted with asterisks and the barred variables denote the typical scales of the variables. For the measurements of the intestinal microbiota used in our analysis, we find typical scales for abundance and time of and. Equation (1) then reads in dimensionless form as, (8) We choose the scale for the perturbation signal such that it is scaled to 1, i. e. . Thus, we obtain the rescaled growth rates, interaction parameters and susceptibilities as, , and and recover the original equation (1) by dropping the asterisks. Given this choice, the (rescaled) parameters of growth and susceptibility are found to be scale-invariant of changes in the typical abundance, in contrast to the interaction parameter. Input variable is one longitudinal data-set in time points with abundances of taxonomic units (in the following analysis, genera), , and time-dependent perturbations represented by their signal. The parameters of interest are the growth, interaction and susceptibility parameters, , and. The operational taxonomic units counts per sample and relative phylogenetic profile as presented in [19] were used as input data for our analysis. As described in the Results section, we considered the ten most abundant genera (including the pathogen C. difficile) and a group “Other” containing the remaining lower abundance genera. The particular grouping was used to reduce sparsity in the data matrix and to avoid spurious, presumably noise-driven contributions. The choice of using the genus level for phylogenetic resolution was dictated by the fact that 1) it is consistent with the original published paper [19] and 2) it represents the most specific phylogenetic level for which we have classification data. In our grouping, we denote a microbial genus “undefined” (abbreviated with “und. ”) when the phylogenetic classification was non-ambiguous up to a certain phylogenetic level. In contrast to Buffie et al. [19] in which the data of the three replicates are presented by their average, we use the individual nine time courses from the cecum (three from each colony) and concatenate their compositions spanning 86 time points into the data matrices and. In case of non-detection of an otherwise present genus, we assign a uniformly distributed random value between zero and the detection limit of the corresponding sample. Whenever a genus is completely absent from all considered samples in a particular inference, its corresponding row in the data matrix of above is set to zero. The perturbation signal for clindamycin is modeled by a unit pulse of length day centered on the time of antibiotic administration. Subsequently, the inference was performed as described above with, i. e. in every round of cross-validation, six of the nine time courses were used as training and the remaining three as test set. Ten rounds of cross-validation yielded the minimizing regularization parameter. The result for using all the data of nine time courses is presented in Figure 2. In the next step, we predicted the behavior of known trajectories only using their initial compositions and clindamycin application and/or C. difficile inoculation and compared it to the measured values. We used from above to infer, and on six out of the nine trajectories, two from each population. These parameters were used to solve eq. (2) numerically for the remaining three trajectories only providing initial compositions and perturbation profiles and/or C. difficile inoculation. Figure 3 shows the predicted trajectories of the second replicate of each of the three populations using parameters inferred on the remaining six. Moreover, the same parameters were used to assess the stability of the three steady states by linear stability analysis (see above). In Figure 4, we compared these to the final composition of the corresponding measured time courses.
Recent advances in DNA sequencing and metagenomics are opening a window into the human microbiome revealing novel associations between certain microbial consortia and disease. However, most of these studies are cross-sectional and lack a mechanistic understanding of this ecosystem' s structure and its response to external perturbations, therefore not allowing accurate temporal predictions. In this article, we develop a method to analyze temporal community data accounting also for time-dependent external perturbations. In particular, this method combines the classical Lotka–Volterra model of population dynamics with regression techniques to obtain mechanistically descriptive coefficients which can be further used to construct predictive models of ecosystem dynamics. Using then data from a mouse experiment under antibiotic perturbations, we are able to predict and recover the microbiota temporal dynamics and study the concept of alternative stable states and antibiotic-induced transitions. As a result, our method reveals a group of commensal microbes that potentially protect against infection by the pathogen Clostridium difficile and proposes a possible mechanism how the antibiotic makes the host more susceptible to infection.
Ecological Modeling from Time-Series Inference: Insight into Dynamics and Stability of Intestinal Microbiota