Cilia-related proteins are believed to be involved in a broad range of cellular processes. Retinitis pigmentosa GTPase regulator interacting protein 1-like (RPGRIP1L) is a ciliary protein required for ciliogenesis in many cell types, including epidermal keratinocytes. Here we report that RPGRIP1L is also involved in the maintenance of desmosomal junctions between keratinocytes. Genetically disrupting the Rpgrip1l gene in mice caused intraepidermal blistering, primarily between basal and suprabasal keratinocytes. This blistering phenotype was associated with aberrant expression patterns of desmosomal proteins, impaired desmosome ultrastructure, and compromised cell-cell adhesion in vivo and in vitro. We found that disrupting the RPGRIP1L gene in HaCaT cells, which do not form primary cilia, resulted in mislocalization of desmosomal proteins to the cytoplasm, suggesting a cilia-independent function of RPGRIP1L. Mechanistically, we found that RPGRIP1L regulates the endocytosis of desmogleins such that RPGRIP1L-knockdown not only induced spontaneous desmoglein endocytosis, as determined by AK23 labeling and biotinylation assays, but also exacerbated EGTA- or pemphigus vulgaris IgG-induced desmoglein endocytosis. Accordingly, inhibiting endocytosis with dynasore or sucrose rescued these desmosomal phenotypes. Biotinylation assays on cell surface proteins not only reinforced the role of RPGRIP1L in desmoglein endocytosis, but also suggested that RPGRIP1L may be more broadly involved in endocytosis. Thus, data obtained from this study advanced our understanding of the biological functions of RPGRIP1L by identifying its role in the cellular endocytic pathway.
Retinitis pigmentosa GTPase regulator interacting protein 1-like (RPGRIP1L, also known as NPHP8, MKS5, KIAA1005, or Ftm in mouse) is a transition zone protein that has important roles in regulating cilia formation and function [1–5]. Mutations in the RPGRIP1L gene cause Joubert syndrome (JBTS) and Meckel syndrome (MKS) [6,7], two severe ciliopathies that are characterized by central nervous system malformation, cystic kidneys, polydactyly, retinal degeneration, and retinal dystrophy [8]. RPGRIP1L participates in the assembly of the ciliary transition zone, autophagy, and activation of the ciliary proteasome [9], whereas mutant RPGRIP1L interferes with ciliary functions, leading to dysplasia of affected organs [6,7, 10]. In the skin, RPGRIP1L is essential for hair follicle morphogenesis by regulating primary cilia formation and hedgehog signaling [11]. Interestingly, RPGRIP1L is also expressed in interfollicular epidermal keratinocytes, many of which are not ciliated [12], suggesting that RPGRIP1L may exert cilia-independent functions in the skin. Desmosomes are anchoring junctions that are essential for functionalities of tissues that are subjected to constant mechanical stress, such as the skin and the heart. Desmosomal junctions are composed of transmembrane cadherins, desmogleins and desmocollins, and cytoplasmic proteins, including junction plakoglobin (JUP), plakophilins, and desmoplakin (DSP) [13,14]. The adhesion function of desmosomal junctions is dependent on the intercellular anchorage of desmogleins and desmocollins. The assembly and disassembly of the desmosomes is highly dynamic, and intercalates with cellular events associated with the regulation of the cytoskeleton, intracellular trafficking, ubiquitination, and molecular signaling [13]. Forward and reverse genetic studies continue to uncover new players involved in the formation of the desmosomes, which collectively contribute to the establishment of a comprehensive regulatory network of desmosome assembly and homeostasis. Mutations in genes encoding desmosomal proteins can cause a range of heritable disorders that affect the skin, hair, and heart, such as monilethrix, woolly hair, palmoplantar keratoderma, and arrhythmogenic right ventricular cardiomyopathy [15–19]. Moreover, disruption of desmosomal junctions by autoantibodies can cause pemphigus, a family of devastating autoimmune disorders characterized by severe intraepithelial blistering in the skin or mucous membranes [20,21]. Loss of desmosomal proteins has, at least in some cases, been linked to cancer development or progression [20,22]. Understanding the cellular and molecular mechanisms underlying the assembly and disassembly of desmosomal junctions is important for the understanding of the pathogenesis of desmosome-related disorders. In this study, we uncovered a previously unknown function of RPGRIP1L in the formation of the desmosomal junctions. We found that disrupting the Rpgrip1l gene in mice or keratinocyte cell lines resulted in desmosomal abnormalities that are associated with aberrant internalization of desmogleins. These findings revealed RPGRIP1L as a regulator of desmosome formation and function, and suggested a broader role of RPGRIP1L in the assembly of cellular organelles, including the ciliary transitional zone and the desmosome.
Rpgrip1l is ubiquitously expressed in the skin, including the epidermis, dermis, and hair follicles [11]. In mouse epidermis, the Rpgrip1l transcript, as determined by in situ hybridization, is consistently expressed in basal epidermal keratinocytes and, to a lesser extent, in spinous and granular cells (Fig 1A). The RPGRIP1L protein is enriched between the basal body (marked by gamma-tubulin, γ-Tub) and ciliary axoneme (marked by acetylated α-tubulin, α-Tub) of ciliated basal keratinocytes (S1A Fig), or enriched at the centrioles of unciliated keratinocytes (S1E Fig), but below detection in Rpgrip1l knockout (Rpgrip1l–/–) epidermis (S1I Fig). Since differentiated epidermal keratinocytes are rarely ciliated [12,23,24], the widespread expression pattern of Rpgrip1l in the epidermis raised the possibility that Rpgrip1l performs functions beyond regulating ciliogenesis and ciliary functions. Indeed, skins of 50% of E18. 5 Rpgrip1l–/–embryos (n = 16) exhibited focal intraepidermal blistering, predominantly between basal and suprabasal keratinocytes, marked by KRT14 and KRT1, respectively (Fig 1B, middle panels). In severe cases, cell-cell detachments could also be observed in the spinous and granular layers (Fig 1B, right panels). To further explore this relatively sporadic blistering phenotype, which was not sufficiently characterized in a previous study [11], and to circumvent perinatal lethality associated with severe developmental abnormalities in Rpgrip1l–/–mutants, including exencephaly and ventricular septal defects [2,25], we cultured skins isolated from E18. 5 embryos. Organ-cultured Rpgrip1l–/–skins exhibited widespread blistering between the basal and suprabasal layers (Fig 1D and 1E), suggesting that blistering is progressive as the skin becomes mature. Histologically, this intraepidermal blistering phenotype was not associated with discernable cytolysis of keratinocytes, detachment of the basement membrane (Fig 1C), or apoptosis (S2 Fig). Thus, these findings suggest that the blistering phenotype observed in Rpgrip1l–/–skin may be associated with abnormalities in keratinocyte adhesion. The desmosomal junctions play essential roles in epidermal adhesion and were, therefore, examined in Rpgrip1l–/–mutants. Immunofluorescence labeling revealed reduced expression of desmoglein 1 (DSG1), desmoglein 3 (DSG3), JUP, and desmocollin 1 (DSC1) in Rpgrip1l–/–skin (Fig 2 and S3 Fig). Specifically, these proteins were diffusely localized to the cytoplasm of keratinocytes of Rpgrip1l–/–skin, in contrast to the predominant plasma membrane localization in control skins (Fig 2 and S3 Fig). The expression patterns of DSP and desmocollin 2 and 3 (DSC2/3) appeared slightly perturbed in Rpgrip1l–/–skin, whereas the expression of plakophilin 1 (PKP1) did not seem to change, as judged by immunofluorescence microscopy (Fig 2 and S3 Fig). Transmission electron microscopy (TEM) revealed that desmosomes between basal and suprabasal keratinocytes were significantly shorter in Rpgrip1l–/–mutants (Fig 3A and 3B). Moreover, in Rpgrip1l–/–skin, the electron dense midline of desmosomes was less prominent or invisible, the keratin filament attachment was reduced, and the outer electron dense plaque appeared less dense or disorganized (Fig 3A). Similar defects were occasionally observed between spinous keratinocytes (Fig 3A). These findings demonstrated that the blistering phenotype in Rpgrip1l–/–skin is correlated with abnormalities in the desmosomal junctions. Interestingly, adherens junctions, as assessed by immunofluorescence labeling of E-cadherin (CDH1), α-catenin (CTNNA1), and β-catenin (CTNNB1), exhibited only subtle perturbations in Rpgrip1l–/–skin (S3 and S4 Figs). Taken together, these data suggest that RPGRIP1L may be required for keratinocyte adhesion primarily through regulating desmosomal junction formation in vivo. RPGRIP1L is expressed in many cell types, and is enriched at the base of cilia for its cilia-related functions [1,2, 6,7, 10,26]. To evaluate the roles of RPGRIP1L in desmosome formation, we first examined the expression pattern of RPGRIP1L in HaCaT cells and normal human epidermal keratinocytes (NHEKs). HaCaT cells do not form primary cilia, and NHEKs rarely form primary cilia (3. 4 ± 2. 6%) after serum starvation, in comparison to mouse embryonic fibroblasts (MEFs) which do (66. 7 ± 12. 5%) (Fig 4A). In HaCaT cells and NHEKs, RPGRIP1L is enriched at the centrosomes (marked by γ-TUB) and diffusely distributed in the cytoplasm (Fig 4A). These findings suggest that the potential role of RPGRIP1L in desmosome formation is independent of its role in ciliogenesis in keratinocytes. We subsequently knocked down the endogenous RPGRIP1L gene in HaCaT cells by siRNAs (Fig 4B and 4C). Knockdown cells were then treated with high calcium to allow desmosomal junctions to form, then subjected to dispase dissociation assay as illustrated in Fig 4D. RPGRIP1L-knockdown markedly compromised the integrity of the epidermal sheet, resulting in significantly increased fragmentation (Fig 4E and 4F). This experiment indicated that RPGRIP1L is functionally required for cell-cell adhesion of keratinocytes in vitro. To further confirm in vivo findings, desmosomal junctions were evaluated in RPGRIP1L-knockdown HaCaT cells and NHEKs. RPGRIP1L-knockdown did not significantly affect cell viability (S5 Fig), but resulted in marked reduction of desmoglein 1 and 2 (DSG1/2) and desmoglein 3 (DSG3) proteins as determined by western blotting (Fig 5A and S6 Fig), but not mRNA (S7 Fig). The protein levels of DSP, PKP1, plakophilin 2 (PKP2), and JUP were unaffected, whereas those of DSC2/3 increased in RPGRIP1L-knockdown cells (Fig 5A and S6 Fig). Immunofluorescence labeling demonstrated that the membrane localization of many desmosomal proteins, including DSG1/2 and DSG3, were significantly reduced in RPGRIP1L-knockdown cells (Fig 5B). These findings suggest that disrupting RPGRIP1L expression in keratinocytes impairs the stability and membrane localization of desmosomal proteins. At the ultrastructural level, RPGRIP1L-knockdown HaCaT cells exhibited desmosomal abnormalities that are similar to those observed in vivo, including disrupted midline and reduced keratin attachment (Fig 5C). Taken together, these in vitro results further substantiated the role of RPGRIP1L in maintaining structural integrity of the desmosomes. In contrast, RPGRIP1L-knockdown did not result in discernable changes in the expression pattern of intermediate filaments in HaCaT cells as demonstrated by KRT14 immunostaining (S8 Fig). Because disrupting Rpgrip1l resulted in consistent changes in the desmogleins under both in vivo and in vitro conditions, and the blistering phenotype observed in Rpgrip1l–/–skins is similar to what is seen in pemphigus, a severe blistering disorder caused primarily by the disruption of the desmogleins, we focused our investigation on the desmogleins. The formation of desmosomes is highly dynamic and can be arbitrarily divided into the assembly and disassembly phases. In HaCaT cells, desmosomes start to assemble when the cells are exposed to high calcium. We found that knocking down RPGRIP1L during desmosome assembly (0. 5,1, and 3 hours after shifting to high calcium, as illustrated in Fig 6A) did not impair the accumulation of DSG1/2 to the plasma membrane, as determined by immunofluorescence labeling (Fig 6B and quantification in 6C), suggesting that RPGRIP1L might be dispensable for desmosome assembly. In contrast, in the disassembly assay (as illustrated in Fig 6D), where DSG1/2 and DSG3 were examined 24 hours after calcium switch, in conjunction with 1-hour EGTA treatment to further induce desmosome disassembly, the plasma membrane localization of DSG1/2 or DSG3 was significantly decreased in RPGRIP1L-knockdown cells such that DSG1/2 or DSG3 appeared discontinuous along, or in some cases absent from the plasma membrane (Fig 6E and 6G, respectively). Quantifications of membrane and cytoplasmic signal intensity showed that the membrane/cytoplasmic ratio of DSG1/2 or DSG3 was significantly reduced in knockdown cells, a phenotype that was further exacerbated upon EGTA treatment (Fig 6F and 6H, respectively). These findings suggest that loss of RPGRIP1L may cause increased internalization of cell surface desmogleins. This result is consistent with a well-established model in which increased desmoglein endocytosis leads to a decrease in both cell surface and steady-state levels of desmogleins, as seen in Figs 2 and 5 and S6B Fig [27,28]. Desmoglein internalization is mediated by multiple endocytic mechanisms and remains a subject of further investigation [29–37]. Nevertheless, blocking endocytosis could prevent the internalization of desmogleins that are present on the cell surface. Here, we utilize two well-established approaches to blocking endocytosis to determine whether aberrantly accelerated internalization of desmogleins in RPGRIP1L-difficient cells is functionally responsible for the loss of membrane desmogleins. One approach was to use dynasore, a specific inhibitor of dynamin GTPase activity [38,39], to suppress endocytosis. Dynasore had been shown capable of stabilizing desmosomal junctions through blocking endocytosis [38]. The other approach was to use hyperosmotic sucrose to suppress endocytosis [40]. Dynasore or sucrose was added 24 hours after shifting to high calcium and 2 hour prior to fixation, as illustrated in Fig 7A. Pretreating RPGRIP1L-knockdown cells with 50 μM dynasore for two hours was sufficient to rescue the increased internalization of DSG1/2, as determined by immunofluorescence labeling (Fig 7B). Specifically, the membrane localization of DSG1/2 in RPGRIP1L-knockdown cells was restored to a level comparable to that of control knockdown (Fig 7B, upper panels, and quantifications in c). Furthermore, dynasore treatment also overcame the additive effects of both knockdown- and EGTA-induced DSG1/2 internalization (Fig 7B, lower panels, and quantifications in 7C). Similarly, hyperosmotic sucrose effectively rescued RPGRIP1L knockdown-induced DSG1/2 internalization in HaCaT cells, even in the presence of EGTA, as demonstrated by immunofluorescence labeling and quantification (Fig 7B, right panels, and 7C). Collectively, these rescue experiments suggest that elevated endocytosis is functionally responsible for RPGRIP1L knockdown-induced internalization of the desmogleins. To directly monitor the endocytosis of desmogleins, we performed an internalization assay, in which the internalized DSG3 can be quantified by labeling DSG3 with AK23, a monoclonal antibody against the extracellular domain of DSG3 [41], in live cells as previously demonstrated [29]. Specifically, as outlined in Fig 7D, AK23 was used to label cell surface DSG3 on ice, one hour prior to shifting to 37°C to trigger internalization (in the presence of control or PV IgG). One hour later, AK23-labeled but not internalized DSG3 was stripped off cell surface through acid wash. Cells were then fixed, and the internalized/AK23-labled DSG3 was detected by immunofluorescence. In control siRNA-treated cells, control IgG treatment resulted in low levels of internalization of DSG3 (Fig 7E, upper left panel). As expected, PV IgG induced marked internalization of DSG3 (Fig 7E, upper right panel, and quantifications in 7F). Remarkably, RPGRIP1L-knockdown also resulted in marked internalization of DSG3 (Fig 7E, lower left panel), an effect as robust as PV IgG, as quantified in Fig 7F, reinforcing prior findings that the loss of RPGRIP1L can be pathogenic. More interestingly, DSG3 internalization was further increased by dual PV IgG and RPGRIP1L siRNA treatments (Fig 7E, lower right panel, and quantifications in 7F). This additive effect suggested that loss-of-RPGRIP1L and PV IgG may promote DSG3 endocytosis through distinct mechanisms. The above findings established a role of RPGRIP1L in regulating the endocytosis of cell surface desmogleins in epidermal keratinocytes. To determine whether RPGRIP1L might be more broadly involved in endocytosis, we evaluated the steady state-rate of endocytosis of cell-surface proteins by biotinylation assays [42]. First, the level of DSG3 decreased in whole cell lysate of RPGRIP1L-knockdown cells (Fig 7G), whereas biotinylated (endocytosed) DSG3 markedly increased in RPGRIP1L-knockdown cells (Fig 7G), a finding that is consistent with the above data (Figs 5B, 6G and 7E). The total protein level of DSC3 increased, whereas biotinylated (endocytosed) DSC3 increased marginally in this biotinylation assay (Fig 7G), also consistent with previously obtained data (Fig 5A and 5B). Next, we examined cell membrane-associated proteins other than desmogleins, specifically epidermal growth factor receptor (EGFR) and CDH1. Despite the comparable total levels of EGFR and CDH1 in control and RPGRIP1L-knockdown cells (Fig 7G, left panels), RPGRIP1L-knockdown correlated with increased levels of biotinylated (endocytosed) EGFR and CDH1 (Fig 7G, right panels). Although the desmogleins cross-regulate with many cell surface proteins, including EGFR [43,44] and CDH1 [45–47], increased endocytosis of EGFR and CDH1 in association with RPGRIP1L-knockdown nevertheless suggests that RPGRIP1L may regulate endocytosis more broadly, which is worthy of future investigation. Collectively, data obtained from this study suggest that increased endocytosis of desmogleins is primarily responsible for the keratinocyte adhesion defects associated with RPGRIP1L deficiency, thereby establishing a role of RPGRIP1L in stabilizing the desmosomes in skin.
Increasing evidence suggests that cilia-related proteins perform important cellular functions beyond regulating cilia formation or function [48]. In this study, we demonstrated that a ciliary protein, RPGRIP1L, is required for the maintenance of desmosomal junctions through regulating endocytosis of desmogleins in epidermal keratinocytes, thereby extending the functions of cilia-related proteins to cell-cell adhesion. Intriguingly, JBTS and MKS patients, who harbor loss-of-function mutations in the RPGRIP1L gene, do not exhibit blistering phenotypes. It is possible that blistering in these patients is underdiagnosed, or that abnormalities in desmosomal junctions exist but are subclinical. It is also plausible that the mutant RPGRIP1L gene products in patients retain a certain level of functionality, whereas genetically disrupting the Rpgrip1l locus leads to more catastrophic phenotypes in mice, by which blistering was observed. Further understanding the molecular mechanisms through which RPGRIP1L participates in desmosomal junction formation will shed light on how RPGRIP1L performs diverse functions in ciliogenesis and desmosome formation. RPGRIP1L is highly enriched at the transition zone of cilia, but is also distributed in the cytoplasm and at the plasma membrane [6,49]. Thus, our finding that RPGRIP1L performs functions beyond the cilia is not entirely surprising. Data obtained from this study support a role of RPGRIP1L in stabilizing desmogleins at the plasma membrane, thus qualifying RPGRIP1L as a regulator of desmoglein internalization. The precise molecular mechanism through which RPGRIP1L regulates desmoglein internalization remains to be uncovered. Without a strong presence at the plasma membrane, it is unlikely that RPGRIP1L interacts with desmosomal proteins at the cell membrane as previously described for Lis1, adducin, and flotillins [50–52]. Rather, in keratinocytes, RPGRIP1L is enriched at the base of the cilium as well as the centrosomes, both hubs for intracellular trafficking. RPGRIP1L may modulate desmoglein internalization by interacting with cytoplasmic regulators of the desmosomes, or through signaling, such as PKCα [37,38,53–56] or p38/MAPK [30,57–59]. Because keratinocyte proliferation, differentiation, and apoptosis are not markedly impaired in RPGRIP1L-deficient cells, the mechanism through which RPGRIP1L regulates desmoglein internalization is likely to be specific to the desmosomal regulatory network. It is well established that RPGRIP1L physically interacts with NPHP4 during cilia formation or function [7,49,60]. NPHP4 is not only required for proper cilia formation, but also implicated in the formation of tight junctions [61]. In the current study, we provided evidence that RPGRIP1L is required for the proper formation and function of the desmosomal junctions, primarily through regulating endocytosis of desmogleins. Interestingly, we also observed, albeit inconsistently, changes in components of adherens junctions, including CDH1, CTNNA1, and CTNNB1. Considering the well-documented cross-talk between these intercellular anchoring junctions [62,63], we postulate that the desmosomes are the primary targets of RPGRIP1L in keratinocytes. This evidence nevertheless raised the possibility that RPGRIP1L may be more broadly involved in cell-cell junctions through the RPGRIP1L-NPHP4 protein complex [7,49]. The functional requirement of NPHP4 or the RPGRIP1L-NPHP4 protein complex in desmosome formation remains to be determined. The desmogleins were consistently the most markedly endocytosed proteins. In unbiased biotinylation assays, elevated internalization of DSG3 was also correlated with increased endocytosis of other cell surface proteins in RPGRIP1L-knockdown cells. It remains to be determined whether this is a mere correlation, or whether RPGRIP1L is functionally associated with endocytosis of other cell surface molecules. Generalization of the potential role of RPGRIP1L in internalization of membrane molecules may further our understanding of endocytosis and recycling in both ciliated and unciliated cells. Given that the current knowledge of the molecular functions of RPGRIP1L is limited to its role in proteasome activity and autophagy at the base of the cilium [26,64], it is plausible that RPGRIP1L also participates in endocytosis through regulating protein degradation, a potential mechanism that needs to be further dissected. The current study focused on the desmogleins, whose expression levels and membrane localizations were found to be consistently compromised in vivo and in vitro, mimicking key pathological features observed in pemphigus. We found that components of the desmosome were not equally affected in Rpgrip1l–/–skin and RPGRIP1L-knockdown cells. The levels of most other desmosomal proteins remained unchanged with the exception of DSC2/3 increasing, whereas PKP1 did not exhibit increased internalization in RPGRIP1L-knockdown cells. It is plausible that the increased DSC2/3 might have helped PKP1 to associate with the cell membrane in RPGRIP1L-deficient cells. In light of these findings, we postulate that desmogleins may be the primary targets of RPGRIP1L, whereas changes in other desmosomal components are secondary or compensatory. Indeed, several prior studies demonstrated the protective role of plakophilin in pemphigus or skin fragility models [34,35,65,66]. In conclusion, data obtained from this study implicate RPGRIP1L in the maintenance of desmosomal junctions through restricting desmoglein endocytosis, thereby revealing a cilia-independent function of RPGRIP1L in epidermal keratinocytes.
All procedures related to mice were performed in accordance with the European Directive 2010/63/EU and the French application decree 2013–118 on the protection of animals used for scientific purposes, and were approved by the local ethical committee" Comité d' éthique Charles Darwin" (approval number 2015052909185846), or in accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health of the United States, and approved by the Institutional Animal Care and Use Committee of Stony Brook University (approval number 2012-1974-R2-9. 14. 18-MI). The Rpgrip1l mouse model was described previously [2,4, 5,25]. HaCaT cells were transfected with RPGRIP1L siRNAs (HSC. RNAI. N015272. 12. 1 and 2, Integrated DNA Technologies, Coralville, IA). Non-targeting (Negative Control) siRNA (NC-1, Integrated DNA Technologies) was used as control siRNA. Twenty-four hours after transfection, cells were switched to high calcium (1. 5 mM CaCl2) for designated durations. EGTA was used at 2 mM. Dynasore (Sigma-Aldrich, Saint Louis, MO) and sucrose were used at 50 μM and 400 mM, respectively. PV IgG were purified in Dr. Payne’s laboratory and used at 400 μg/ml. Normal human IgG (Sigma-Aldrich, Saint Louis, MO) was used as control IgG. Method details are provided in the Supplementary Materials and Methods (S1 Text) online. Skins excised from E18. 5 embryos were cultured as previously described [67]. Briefly, dorsal skins harvested from E18. 5 embryos were cultured at the air-liquid interface in DMEM and 10% fetal bovine serum at 37° C and 5% CO2. Cultured skins were then fixed in 10% buffered formalin and processed for routine histology analysis. Freshly isolated tissues were fixed immediately in buffered formalin, embedded in paraffin, and processed for routine hematoxylin and eosin (H&E) staining or other examinations. Most immunofluorescence labeling of tissue specimens and cells was performed on formalin-fixed paraffin-embedded tissue sections as described previously [68,69]. RPGRIP1L, cilia, DSG3, and CTNNA1 were detected on frozen sections of the skin (Supplementary Methods). TUNEL staining was performed with DeadEnd Fluorometric TUNEL System (Promega, Fitchburg, WI). Primary antibodies are listed in S1 Table. AlexaFluor-conjugated secondary antibodies (1: 200) were obtained from Life Technologies (Carlsbad, CA). Sections were sealed in mounting medium with or without DAPI (Vector Laboratories, Burlingame, CA). Images were acquired by a Nikon 80i fluorescence microscope, fitted with a Nikon (Melville, NY) DS-Qi1Mc camera, or by a Leica (Wetzlar, Germany) SP5C Spectral confocal laser-scanning microscope, and processed with Photoshop 5. 5 CS (Adobe System Incorporated, San Jose, CA). To quantify fluorescence intensity of skin tissues, the mean intensity of randomly selected epidermal regions (2 regions per specimen, n ≥ 3 mice per group, excluding the cornified layer), was measured with the NIS-Element analysis software, as described elsewhere [50]. To obtain the ratio of membrane/cytoplasmic fluorescence intensity, the mean peak pixel values at the two edges of a cell (representing the plasma membrane) and the mean pixel value between the peaks (representing the cytoplasm) were obtained by the Plot Profile tool of the ImageJ software (1. 43u, National Institute of Health, Bethesda, MD), and presented as a membrane/cytoplasmic ratio. The dispase dissociation assay was performed as described previously [70]. Briefly, 24 hours after transfection, confluent cells were incubated in high calcium medium (1. 5 mM CaCl2) for 24 hours. Subsequently, cells were washed with DPBS and incubated with dispase II (2. 4 U/ml in EMEM with 10% FBS and 1. 5 mM CaCl2, Roche, Indianapolis, IN), for 20 min at 37°C. Detached monolayers were subjected to mechanical challenge by inverting 50 times in 4 ml PBS in a 15-ml Falcon tube. Cell fragments were imaged and counted under a dissecting microscope (Stemi 2000-C, Carl Zeiss, Obserkochen, Germany). The IgG internalization assay was performed to detect internalized DSG3 as previously described [27,29]. Briefly, HaCaT cells were incubated with a monoclonal antibody (AK23) against the extracellular domain of DSG3 [41], in media containing 1. 5 mM calcium for 30 minutes on ice. Cells were then washed and incubated with PV IgG (400 μg/ml) or normal human IgG (400 μg/ml) at 37° C for one hour to induce DSG3 internalization. Subsequently, cells were treated with acid wash solution (100 mM glycine, 20 mM magnesium acetate, 50 mM potassium chloride, pH 2. 2) to remove surface-bound DSG3 antibody before fixation. For knockdown studies, cells were transfected with siRNA prior to calcium switch. Images were acquired by Leica SP5C Spectral confocal laser scanning microscope under the same color intensity threshold and analyzed using ImageJ. Quantification was done by counting green fluorescent puncta in randomly sampled microscopic fields with Analyze Particle, which was then normalized by the number of cells so that the net result reflects the average number of puncta (internalized DSG3) within one cell. Cell surface protein biotinylation and endocytosis assays were used to measure internalization of cell surface proteins, as described previously [42]. Briefly, cells were incubated with freshly prepared 2 mg/ml Sulfo-NHS-SS-biotin (EZ-Link™Sulfo-NHS-SS-Biotin; Thermo Fisher Scientific, Waltham, MA) for 30 min at 4°C after two washes in ice-cold PBS2+ (PBS with 1. 5 mM CaCl2 and 1. 5 mM MgCl2) for biotinylation to occur. Cells were then washed and incubated in three changes of quenching solution (100 mM glycine in PBS2+), 10 minutes each, on ice. After a PBS2+ wash, cells were incubated in the pre-warmed high calcium growth media containing 2 mM EGTA for 30 min to trigger internalization. Stripping control cells were kept at 4°C to block internalization. Subsequently, non-internalized biotin was stripped by washing with cold NT buffer (150 mM NaCl, 1. 0 mM EDTA, 0. 2% BSA, 20 mM Tris, pH 8. 6, and 50 mM Tris (2-Carboxyethyl) phosphine Hydrochloride), and cell lysates were collected in RIPA buffer containing protease inhibitor. Biotinylated proteins were pulled down with streptavidin magnetic beads (Thermo Fisher Scientific) at 4°C overnight, eluted in Laemmli buffer at 95°C, and analyzed by immunoblotting. Rabbit anti-DSG3 (Bio-Rad AbD Serotec, Raleigh, NC) was used to detect biotinylated DSG3. Target proteins were examined in a minimum of three experiments. Results from one representative experiment are shown. All quantifications are presented as mean ± SD. Student’s t-test was used unless otherwise stated. One-way ANOVA and two-way ANOVA were conducted using the GraphPad software. P < 0. 05 was considered statistically significant. Additional Materials and Methods information is provided in the Supplementary Materials and Methods (S1 Text) online.
The desmosome is a type of intercellular junction, essential for cells to adhere to one another. Abnormalities in desmosomes can cause disorders in the hair, skin, and heart, some of which are severe or even fatal. Here, we discovered that RPGRIP1L, a protein known to regulate cilia formation and function, is essential for stabilizing desmosomes of skin keratinocytes. Specifically, suppressing the Rpgrip1l gene in mice or in keratinocytes disrupted the ultrastructure of desmosomes, and compromised cell-cell adhesion in vivo and in vitro. We found that knocking down RPGRIP1L in keratinocytes aberrantly accelerated the internalization of cell membrane desmogleins, key desmosomal cadherins. Inhibiting endocytosis effectively rescued these phenotypes. Biotinylation assays confirmed that desmogleins are likely the primary targets of RPGRIP1L. Interestingly, membrane proteins that are not directly associated with the desmosomes were also found to be internalized in RPGRIP1L-knockdown cells, raising the possibility that RPGRIP1L might regulate endocytosis more broadly. Findings from this study not only identified RPGRIP1L as a regulator of the desmosomes, but also expanded our understanding of cilia-related proteins in the formation of the desmosomal junctions.
cell physiology
keratinocytes
medicine and health sciences
gene regulation
cell processes
junctional complexes
epithelial cells
membrane proteins
immunologic techniques
cellular structures and organelles
research and analysis methods
small interfering rnas
animal cells
gene expression
immunoassays
biological tissue
cell membranes
immunofluorescence
endocytosis
biochemistry
rna
cell biology
anatomy
secretory pathway
nucleic acids
cilia
genetics
epithelium
biology and life sciences
cellular types
non-coding rna
desmosomes
RPGRIP1L is required for stabilizing epidermal keratinocyte adhesion through regulating desmoglein endocytosis