The large conductance, voltage- and calcium-dependent potassium (BK) channel serves as a major negative feedback regulator of calcium-mediated physiological processes and has been implicated in muscle dysfunction and neurological disorders. In addition to membrane depolarization, activation of the BK channel requires a rise in cytosolic calcium. Localization of the BK channel near calcium channels is therefore critical for its function. In a genetic screen designed to isolate novel regulators of the Caenorhabditis elegans BK channel, SLO-1, we identified ctn-1, which encodes an α-catulin homologue with homology to the cytoskeletal proteins α-catenin and vinculin. ctn-1 mutants resemble slo-1 loss-of-function mutants, as well as mutants with a compromised dystrophin complex. We determined that CTN-1 uses two distinct mechanisms to localize SLO-1 in muscles and neurons. In muscles, CTN-1 utilizes the dystrophin complex to localize SLO-1 channels near L-type calcium channels. In neurons, CTN-1 is involved in localizing SLO-1 to a specific domain independent of the dystrophin complex. Our results demonstrate that CTN-1 ensures the localization of SLO-1 within calcium nanodomains, thereby playing a crucial role in muscles and neurons.
Precise control of membrane excitability, largely determined by ion channels, is of utmost importance for neuronal and muscle function. The regulation of ion channel localization, density and gating properties thus provides an effective way to control the excitability within these cells [1]. Indeed, the localization and gating properties of ion channels are often regulated or modified by cytoskeletal and signaling proteins, or auxiliary ion channel subunits expressed in a cell-type specific manner [2]. Potassium channels are critical in determining the excitability of cells, because potassium ions are dominant charge carriers at the cell resting potential. Among potassium channels, the large conductance, voltage- and calcium-dependent potassium BK channels (also called SLO-1 or Maxi-K) are uniquely gated by coincident calcium signaling and membrane depolarization [3], [4]. This feature of BK channels provides a crucial negative feedback mechanism for calcium-induced functions, and plays an important role in determining the duration of action potentials [3]. BK channels are widely expressed in a variety of cell types and are implicated in many physiological processes, including the regulation of blood pressure [5], neuroendocrine signaling [6], smooth muscle tone [7], and neural network excitability [8], [9]. Mounting evidence indicates that BK channels can interact with a variety of proteins that modulate channel function, or control membrane trafficking. For example, the Drosophila BK channel, dSLO, interacts with SLO binding protein (slob), which in turn modulates the channel gating properties [10]. Similarly, mammalian BK channels associate with auxiliary beta subunits that influence channel activation time course and voltage-dependence [11]. In yeast two hybrid screens, the cytoplasmic C-terminal tail of mammalian BK channels has been shown to interact with several proteins, including cytoskeletal elements, such as actin-binding proteins [12], [13] and a microtubule-associated protein [14]. These cytoskeletal proteins are partially co-localized with BK channels, and appear to increase cell surface expression of BK channels in cultured cells [12], [13]. However, it remains to be determined whether these proteins have any role in controlling the localization of BK channels to specific areas of the plasma membrane in vivo. Robust activation of BK channels requires higher intracellular calcium concentrations (>10 µM), which only occur in the immediate vicinity of calcium-permeable channels [4]. Hence, the localization of BK channels to specific areas (i. e. calcium nanodomains) where calcium-permeable ion channels are located is physiologically important for BK channel activation. In C. elegans, loss-of-function mutations in slo-1 partially compensate for the synaptic release defects of C. elegans syntaxin (unc-64) mutants [15] and lead to altered alcohol sensitivity [16]. Recent studies in C. elegans have also implicated SLO-1 in muscle function [17]. slo-1 mutants display an exaggerated anterior body angle, referred to as the head-bending phenotype that is shared by mutants that are defective in the C. elegans dystrophin complex [18]–[20]. Recent evidence that the C. elegans dystrophin complex interacts with SLO-1 channels via SLO-1 interacting protein, ISLO-1, explains this phenotypic overlap [21]. However, C. elegans dystrophin complex mutants do not appear to alter the biophysical properties of BK channels per se [17]. Similarly, ISLO-1 does not modify SLO-1 channel properties [21]. Rather, ISLO-1 tethers SLO-1 near the dense bodies of muscle membranes, where L-type calcium channels (EGL-19) are localized [21]. Consequently, defects in the dystrophin complex or ISLO-1 cause a large reduction in SLO-1 protein levels in muscle membrane, which in turn causes muscle hyper-excitability leading to enhanced intracellular calcium levels. This perturbation of calcium homeostasis has been postulated to be one of the first steps in the degenerative muscle pathogenesis associated with disruption of the dystrophin complex [22]. In this study, we performed a forward genetic screen to identify additional genes responsible for SLO-1 localization and function in C. elegans. We identified ctn-1, an orthologue of α-catulin, as a novel gene that controls SLO-1 localization and function in muscles and neurons. Our analysis showed that ctn-1 uses different strategies to localize SLO-1 in these two cell types. In muscles, CTN-1 utilizes the dystrophin complex to localize SLO-1 near L-type calcium channels via ISLO-1. In neurons, CTN-1 localizes SLO-1 independent of the dystrophin complex.
Loss-of-function slo-1 mutants exhibit a jerky locomotion and head bending phenotype [15]. By contrast, gain-of-function slo-1 mutants exhibit sluggish movement combined with low muscle tone [16]. When slo-1 (gf) mutant animals are mechanically stimulated, they fail to make a normal forward movement, and tend to curl ventrally (Video S1). To identify genes that regulate slo-1 function, we performed a forward genetic screen to isolate mutants that suppress the phenotypes of the slo-1 (ky399) gain-of-function mutant. Based on a previous genetic study [21], suppressor genes were expected to encode slo-1, components of the dystrophin complex, as well as novel proteins that control neuronal or muscular function of SLO-1. As expected, several loss-of-function alleles of slo-1 were isolated. In addition to these intragenic suppressors, several mutants could be segregated away from slo-1 (gf) (Figure 1A and Video S1) and exhibited the head bending phenotype. Genetic mapping and complementation testing determined that these extragenic suppressors include dyb-1 and stn-1 which encode two homologous components of the dystrophin complex, dystrobrevin and syntrophin respectively. Additionally we isolated cim6 and eg1167 suppressors that represent novel genes. Compared to slo-1 (ky399) and cim6; slo-1 (ky399) mutants, eg1167; slo-1 (ky399) mutants exhibited a profound improvement in the locomotion speed (Figure 1A). It was previously observed that slo-1 (gf) mutants retain significantly more eggs than wild-type animals due to low activity of the egg-laying muscles [16]. We found that suppressor mutants abolish an egg laying defect of slo-1 (gf) mutants and retain eggs in uteri at levels similar to wild-type animals (Figure 1B). To understand the role of novel genes in slo-1 function, we pursued the identification of genes that mapped to chromosomal locations neither previously implicated in BK channel function, nor encoding known components of the dystrophin complex. Two mutations, cim6 and eg1167, both mapped to the left side of chromosome I and failed to complement each other for head bending, suggesting that these two mutations represent alleles of the same gene. Our quantitative analysis for locomotion and egg laying phenotypes showed that the locomotion speed of eg1167; slo-1 (gf) was higher than that of cim6; slo-1 (gf) whereas egg laying was comparable in both strains (Figure 1A and 1B). We further mapped eg1167 to a 250 kb interval and rescued the phenotype of eg1167 by generating transgenic animals with the fosmid WRM0621cC01 (Figure S1). Next, we rescued the head bending phenotype of eg1167 with a transgene consisting of the ctn-1 gene (Y23H5A. 5) and approximately 4 kb upstream of the translation initiation codon (Figure 2A and 2B). The same transgene caused eg1167; slo-1 (gf) double mutants to revert to the slo-1 (gf) phenotype, displaying sluggish movement and retention of late-staged eggs in uteri (Figure 2C and 2D). These results indicate that a genetic defect in ctn-1 is responsible for suppression of the slo-1 (gf) phenotypes. The ctn-1 gene is orthologous to mammalian α-catulin (39. 4% identity to human α-catulin), and is named on the basis of sequence similarity to both α-catenin and vinculin (Figure 2A) [23]. Vinculin and α-catenin are membrane-associated cytoskeletal proteins found in focal adhesion plaques and cadherens junctions. In C. elegans, vinculin (DEB-1) is localized to the dense bodies of body wall muscle and is essential for attachment of actin thin filaments to the sarcolemma [24], whereas α-catenin (HMP-1) is localized to hypodermal adherens junctions and is essential for proper enclosure and elongation of the embryo [25]. Based on its homology to vinculin/α-catenin and the localization of mammalian α-catulin [26], CTN-1 is likely to interact with other cytoskeletal proteins, which may in turn affect SLO-1 function. Additionally, the ctn-1 gene encodes a predicted coiled-coil domain. Such a coiled-coil domain mediates the interaction between dystrophin and dystrobrevin [27], two components of the dystrophin complex, although we do not know if the coiled-coil domain of CTN-1 is important for the interaction with these proteins (Figure 2A). We determined nucleotide sequence of the predicted exons and exon-intron boundaries of the ctn-1 gene in eg1167 and cim6. The mutation sites found in both alleles create translation-termination codons (R144>STOP in eg1167, Q521>STOP in cim6) (Figure 2A). eg1167 exhibits complete suppression of slo-1 (gf) phenotypes (see below) and is hence considered as a severe loss-of-function or null allele. All subsequent experiments were carried out with eg1167, unless mentioned otherwise. Although both eg1167 and cim6 mutants alone exhibit the head-bending phenotype, they differ with respect to suppression of slo-1 (gf) phenotypes. Whereas ctn-1 (eg1167) suppresses all aspects of the slo-1 (gf) phenotype, ctn-1 (cim6) completely suppresses the egg-laying defect of slo-1 (gf) (Figure 1B), but not the locomotory defect (Figure 1A). These results suggest that the C-terminal third of CTN-1 is required for normal egg laying and head bending, but is not necessary to mediate the locomotion speed defect of slo-1 (gf) mutants. To elucidate the function of CTN-1, we examined the expression pattern of the ctn-1 gene using a ctn-1 promoter-tagged GFP reporter (Figure 2E–2H). We observed GFP fluorescence in body wall muscles, pharyngeal muscle, egg-laying muscle and enteric muscle of transgenic animals as well as in most, if not all, neurons of the nerve ring and ventral nerve cord. Based on the ctn-1 expression pattern and the phenotypic differences between eg1167 and cim6, we investigated whether the head-bending phenotype and the suppression of sluggish movement of slo-1 (gf) mutants are separable by expressing ctn-1 minigenes under the control of either muscle- or neuron-specific promoters in ctn-1 and ctn-1; slo-1 (gf) mutant animals. Muscle, but not neuronal, expression of ctn-1 rescued the head-bending phenotype of the ctn-1 mutant (Figure 2B and Figure S1C). These results are consistent with previous reports that the head-bending phenotype is due to perturbations in muscle function [17]–[19]. Furthermore, muscle expression of ctn-1 in ctn-1; slo-1 (gf) mutants resulted in egg retention to the level observed in slo-1 (gf) mutants, whereas neuronal expression of ctn-1 did not alter the number of eggs retained in the uteri of ctn-1; slo- (gf) mutants (Figure 2C). Conversely, neuronal expression of ctn-1 in ctn-1; slo-1 (gf) mutants reverted the seemingly normal locomotion of ctn-1; slo-1 (gf) to the sluggish, uncoordinated locomotion of the slo-1 (gf) mutant, whereas muscle expression of ctn-1 did not (Figure 2D). These results indicate that the sluggish, uncoordinated locomotory phenotype of slo-1 (gf) mutants comes from presynaptic depression, but not from direct suppression of muscle excitability. Together with the allele specific phenotypic differences indicating different regions of CTN-1 are required for normal locomotory speed and head bending, these results suggest that CTN-1 uses two distinct mechanisms for mediating SLO-1 function in muscle and neurons by interacting with different sets of genes. Most, if not all, of the mutants that exhibit the head bending phenotype have a defect in either a component of the dystrophin complex or proteins that interact with the dystrophin complex [17]–[19]. The dystrophin complex is localized near muscle dense bodies [21]. Because ctn-1 mutants exhibit the head bending phenotype, we determined the subcellular localization of CTN-1 using a GFP-tagged CTN-1 transgene, which rescues the head bending phenotype (data not shown). GFP: : CTN-1 exhibited a punctate expression pattern that resembled that of the dense bodies (Figure 3A). To further define the localization of CTN-1, we stained GFP-tagged CTN-1 transgenic animals with GFP antibodies and vinculin/DEB-1 antibodies that recognize the attachment plaque and dense bodies. CTN-1: : GFP is localized in close proximity to, or partially colocalized with, vinculin/DEB-1 in dense bodies, but not in the attachment plaques, indicating that CTN-1 is localized near dense bodies (Figure 3A). This expression pattern of CTN-1, along with the head bending phenotype of ctn-1 mutants, prompted us to examine whether the ctn-1 mutation disrupts the integrity of the dystrophin complex. We compared the expression pattern of a component of the dystrophin complex, SGCA-1 (an α-sarcoglycan homolog) in wild-type, dys-1, slo-1 and ctn-1 animals using a GFP-tagged SGCA-1 that rescues the head bending phenotype of sgca-1 mutants [21] (Figure 3B). GFP: : SGCA-1 exhibited a punctate expression pattern in the muscle membrane of wild-type and slo-1 mutant animals. By contrast, GFP puncta were greatly diminished in dys-1 and ctn-1 mutants. These results indicate that ctn-1 is critical for maintaining the dystrophin complex near the dense bodies. We previously demonstrated that ISLO-1 interacts with STN-1 through a PDZ domain-mediated interaction, thereby linking SLO-1 to the dystrophin complex [21]. Because we failed to observe a component of the dystrophin complex in the muscle membrane of ctn-1 mutants, we examined mCherry-tagged ISLO-1 in the muscle membrane of wild-type and ctn-1 mutant animals. The punctate mCherry: : ISLO-1 fluorescence was observed in wild-type muscle membranes, but was greatly reduced in ctn-1 mutant (Figure 3C). These results further strengthen the notion that CTN-1 is required for maintaining the integrity of the dystrophin complex. Based on the genetic interaction between ctn-1 and slo-1, and the observation that the integrity of the dystrophin complex and ISLO-1 localization are disrupted in ctn-1 mutants, we hypothesized that CTN-1 regulates the localization of SLO-1 in muscle. To test this hypothesis, we examined the localization of GFP-tagged SLO-1 in muscles of wild-type, dys-1, and ctn-1 animals (Figure 4A and 4B). The punctate SLO-1: : GFP expression pattern in the muscle membrane of wild-type animals was greatly diminished in the muscles of either dys-1 or ctn-1 mutant. Interestingly, the protein levels of SLO-1: : GFP were not significantly different in wild-type, dys-1 and ctn-1 animals (Figure S2B), indicating that mislocalized SLO-1 does not necessarily undergo degradation. The mislocalization of SLO-1 in dys-1 mutants is consistent with the requirement of the dystrophin complex for ISLO-1 localization [21]. These results further indicate that CTN-1 stabilizes or maintains the punctate muscle expression of SLO-1: : GFP in a dystrophin complex-dependent manner. In mammals, BK channels are found in neuronal somata, dendrites and presynaptic terminals [28], [29]. An immunoelectron microscopy study indicates that BK channels are not homogeneously distributed in neurons, but are clustered, presumably near calcium channels [30]. We addressed whether SLO-1 is evenly distributed or clustered in C. elegans neurons by examining SLO-1: : GFP. Wild-type animals displayed patches of fluorescence along the ventral nerve cord or near cell bodies under high magnification (Figure 4C and 4D, Figure S2). Tissue-specific rescue experiments demonstrated that ctn-1 mediates SLO-1 function in neurons independent of the dystrophin complex (Figure 2D). Therefore, we compared neuronal SLO-1: : GFP expression in dys-1 and ctn-1 mutant animals. The clustered GFP expression observed along the ventral cord of both wild-type and dys-1 mutant animals contrasted with the uniform GFP localization in ctn-1 mutants (Figure 4C and 4D). These results indicate that ctn-1 mutation disrupts the neuron-specific clustering of SLO-1: : GFP independent of the dystrophin complex. SLO-1 contributes to the repolarization of the synaptic terminal following neuronal stimulation, thereby terminating neurotransmitter release. Consequently loss-of-function slo-1 mutants are hypersensitive to the paralyzing effects of aldicarb, an acetylcholinesterase inhibitor, a phenotype indicative of enhanced acetylcholine release. Consistent with this interpretation, electrophysiological recordings from neuromuscular junctions of slo-1 loss-of-function mutants exhibit prolonged evoked synaptic responses [15], [16]. If CTN-1 regulates SLO-1 localization in motor neurons and thus slo-1 function, we would expect ctn-1 mutants to exhibit similar pharmacological and synaptic changes. Indeed, we found that ctn-1 mutants were hypersensitive to aldicarb compared to wild-type animals (Figure S3). To confirm this observation directly, we measured synaptic responses from the neuromuscular junctions of dissected wild-type and ctn-1 mutant animals engineered to express channelrhodopsin-2 in motor neurons [31] (Figure 5). Evoked synaptic responses were elicited by blue light activation of channelrhodopsin-2 and recorded from voltage-clamped post-synaptic body wall muscle cells. Consistent with our pharmacological data and localization results, recordings from ctn-1 showed prolonged evoked synaptic responses similar to those of slo-1 (lf) mutants (Figure 5B and 5C). Furthermore, muscular expression of ctn-1 in ctn-1 mutant animals rescued the head-bending phenotype (Figure 2B), but did not rescue prolonged evoked synaptic responses (Figure S3B). These data strongly suggest that altered synaptic responses of ctn-1 mutants result from a neuronal defect. In contrast to the slo-1 (lf) mutants, evoked responses of slo-1 (gf) mutants were short-lived (Figure 5C), and the charge integral, a measure of total ion flux during the evoked response, was significantly reduced (Figure 5E). Our genetic analyses demonstrated that the ctn-1 mutation suppresses the sluggish locomotory phenotype of slo-1 (gf) mutants and disrupts SLO-1 localization (Figure 1A, Figure 4C and 4D). If this is due to loss of neuronal SLO-1 (gf) channels, the ctn-1 mutation should suppress the evoked response defects of slo-1 (gf). Consistent with this prediction, the decay time of the ctn-1; slo-1 (gf) double mutants (t1/2 = 6. 61±0. 53 ms) was significantly longer than slo-1 (gf) (t1/2 = 3. 23±0. 21 ms) (Figure 5D), and the charge integral was restored to wild-type levels (Figure 5E). Interestingly, ctn-1 mutants did not convert the decay time of slo-1 (gf) evoke responses to that of slo-1 (lf), indicating that residual SLO-1 function may be mediated by dispersed SLO-1 channels.
In a genetic screen to identify novel regulators of SLO-1, we found two alleles of ctn-1, a gene which encodes an α-catulin orthologue. CTN-1 mediates normal bending of the anterior body through SLO-1 localization near the dense bodies of body wall muscles. CTN-1 also maintains normal locomotory speed through SLO-1 localization within neurons. Based on our data, we propose a model for ctn-1 function in localizing SLO-1 (Figure 6). In muscles, CTN-1 interacts with the dystrophin complex. It is also possible that CTN-1 may influence the stability of another protein that directly interacts with the dystrophin complex. Loss of CTN-1 function disrupts the integrity of the dystrophin complex, thus compromising ISLO-1 and SLO-1 localization near muscle dense bodies, where L-type calcium channels are present. Disruption of SLO-1 localization is expected to uncouple local calcium increases from SLO-1-dependent outward-rectifying currents, resulting in muscle hyper-excitation. Previous studies have shown that the head bending phenotype, shared among mutants that have a defect in the dystrophin complex or its associated proteins, results from muscle hyperexcitability [17]–[19], [32]. Our data further show that this head-bending phenotype does not result from a synaptic transmission defect, but from a muscle excitation and contraction defect. In neurons, SLO-1 localization is not mediated through the dystrophin complex, suggesting that CTN-1 interacts with other proteins to localize SLO-1 to specific neuronal domains. Why does CTN-1 use two distinct mechanisms to localize SLO-1 to subcellular regions of muscles and neurons? BK channels are functionally coupled with several different calcium channels (including voltage-gated L-type and P/Q-type calcium channels and IP3 receptors) that are localized in different subcellular regions [30], [33], [34]. Although it has not been determined whether all of these calcium channels are functionally coupled with SLO-1 in C. elegans, these calcium channels are distributed in different regions of neurons. For example, the L-type calcium channel (EGL-19) is mainly expressed in the cell body and the P/Q type calcium channel (UNC-2) is concentrated at the presynaptic terminals [35], [36]. A distinct set of proteins is perhaps required for SLO-1 channel localization near different calcium channels. How CTN-1 interacts with the dystrophin complex in muscle remains to be determined. It has been suggested that mammalian α-catulin interacts with the hydrophobic C-terminus of dystrophin resulting from alternative splicing [37]. However, the C. elegans dys-1 gene does not encode a hydrophobic C-terminus. Thus, CTN-1 may interact with a different domain of dystrophin, or with another component of the dystrophin complex. In this regard, it is noteworthy that both mammalian dystrophin and C. elegans DYS-1 have multiple spectrin repeat domains, and that the N-terminal region of vinculin which exhibits homology to that of α-catulin (Figure S1) is known to bind the spectrin repeat domain of α-actinin [38]. By extension, we speculate that the N-terminal region of CTN-1 may bind the spectrin repeat domain of DYS-1 directly. Alternatively, the coiled-coil domain of dystrophin, which is known to interact with the coiled-coil domain of dystrobrevin [27], may potentially bind the coiled-coil domain of CTN-1. Interestingly, CTN-1 exhibits high homology to vinculin in both the N-terminal and C-terminal regions (Figure S1B). The C-terminal region of vinculin interacts with cytoskeletal molecules or regulators (F-actin, inositol phospholipids and paxillin) in focal adhesion and adherens junctions [39]. Because the C-terminal region of CTN-1 is also necessary for normal head bending, we speculate that this C-terminal region may be important for tethering the dystrophin complex to other cytoskeletal proteins. In mammalian striated muscle, dystrophin is enriched in costameres [40] which are analogous to C. elegans dense bodies. A costamere is a subsarcolemmal protein assembly that connects Z-disks to the sarcolemma, and is considered to be a muscle-specific elaboration of the focal adhesion in which integrin and vinculin are abundant. Compromised costameres have been postulated to be an underlying cause of several different myopathies [40]. It was recently shown that ankyrin-B and -G recruit the dystrophin complex to costameres [41]. Based on overall high homology of ctn-1 to vinculin and α-catenin, we speculate that CTN-1 similarly interacts with cytoskeletal proteins in the dense bodies, and links the dystrophin complex to the dense bodies. Another intriguing conclusion from our data is that loss of CTN-1 does not completely abolish SLO-1 function. Complete abolishment of SLO-1 function in ctn-1 mutant should alter the decay time for evoked synaptic responses of ctn-1; slo-1 (gf) to the same degree as slo-1 (lf) mutants, rather than to that of wild-type animals (Figure 5D). Mutants including slo-1 (gf), that have defects in neural activation or membrane depolarization, are reported to cause str-2, a candidate odorant receptor gene, to be expressed in both AWC olfactory neurons whereas wild-type animals express str-2 in only one of the AWC pair [42]. We find that ctn-1 mutation does not suppress the misexpression of str-2 in both AWC neurons in slo-1 (gf) mutants, suggesting that ctn-1 mutations do not completely abolish SLO-1 function (unpublished observations, HK). It is thus likely that the defect in SLO-1 localization in ctn-1 mutants makes it less responsive to local calcium nanodomains found at presynaptic terminals and dense bodies, but still able to respond to depolarization-induced global calcium increases, albeit at a lower level. In conclusion, we have identified ctn-1, a gene encoding the C. elegans homolog of α-catulin, and demonstrated that CTN-1 mediates SLO-1 localization in muscles and neurons by dystrophin complex-dependent and -independent mechanisms, respectively. How SLO-1 is localized to certain neuronal domains will require further screening of slo-1 (gf) suppressor mutants. Given that proteins affecting components of the dystrophin complex are likely to contribute to the pathogenesis of muscular dystrophy, α-catulin is a candidate causal gene for a form of muscular dystrophy in humans.
The genotypes of animals used in this study are: N2 (wild-type), CB4856, dys-1 (eg33) I, stn-1 (tm795) I, ctn-1 (eg1167) I, ctn-1 (cim6) I, slo-1 (eg142) V, slo-1 (ky399gf) V and sgca-1 (tm1232) X. The following transgenes were used in this study: cimIs1[slo-1a: : GFP, rol-6 (d) ] [21], cimIs5[mCherry: : islo-1, ofm-1: : GFP] [21], zxIs6[unc-17: : chop-2 (H134R) -yfp; lin-15 (+) ] [31], cimIs6[GFP: : sgca-1, rol-6 (d) ], cimEx5[ctn-1, ofm-1: : GFP], cimIs7[GFP: : ctn-1, rol-6 (d) ], cimEx6[Pmyo-3ctn-1, Pmyo-3GFP, ofm-1: : GFP] and cimEx7[PH20ctn-1, PH20GFP, ofm-1: : GFP]. Gain-of-function slo-1 (ky399) mutants were mutagenized by exposure to 50 mM EMS (ethane methyl sulfonate) for 4 h [43]. Suppressors that suppress or ameliorate the sluggish locomotory phenotype of slo-1 (ky399gf) mutants were selected from F2 progeny of the mutagenized animals. We screened approximately 5,000 haploid genome size for suppressor mutants and identified a total of 17 suppressor mutants. Genetic analysis of these suppressor mutants indicates that three of these have a second mutation in the slo-1 gene. In addition, we found that eight have mutations in genes causing head-bending phenotype (2 alleles of dyb-1,3 alleles of stn-1 and 2 alleles of ctn-1). The remaining six mutants do not exhibit distinct locomotory phenotypes when segregated from slo-1 (gf). For genetic mapping, slo-1 (ky399) mutants were outcrossed 12 times to the CB4856 strain. The resulting strain was used for SNP (single nucleotide polymorphism) mapping [44]. Alternatively, we used CB4856 as a mapping strain when mapping is based on the head-bending phenotype. For transgenic rescue, fosmid clones purchased from Gene services Inc. (Cambridge, UK) were injected into the gonad of ctn-1 mutant at 2 ng/µl along with ofm-1: : GFP marker (30 ng/µl). Once we rescued the head bending phenotype of ctn-1 with a single fosmid, we rescued ctn-1 mutant with a genomic DNA fragment encompassing the entire coding sequence of ctn-1 and approximately 4 kb upstream of the putative translation site. To verify the predicted coding sequence of ctn-1, we first performed BLAST search analysis using the genomic sequences of C. briggsae and C. remanei. This analysis suggested that the first and 12th exons are longer than predicted in WormBase (WS208), and that an additional exon (10th exon) is present. Second, we sequenced C. elegans ORF ctn-1 clone (9349620) and confirmed the 10th and the 12th exon sequences. Third, we performed sequence analysis of the DNA fragment obtained from RT-PCR with a primer set (SL1 and an internal primer) and identified the trans-splicing site which is 29 bp upstream of the newly-defined translation initiation site. Our analysis indicates that ctn-1 encodes a predicted protein with 784 amino acids (Figure S1B). The ctn-1 genomic DNA (approximately 4 kb upstream of the promoter and the entire coding sequence) was amplified by the expand long template PCR system (Roche Applied Science) and used directly for rescue. For PH20ctn-1 and Pmyo-3ctn-1 constructs, the neuron-specific H20 [45] or muscle-specific myo-3 promoter sequences were fused to the translation initiation site of the ctn-1 genomic DNA in frame by the overlapping extension PCR (Roche). For localization of CTN-1, we inserted the GFP sequence to the translation initiation site of ctn-1 cDNA, and then the ctn-1 promoter sequence was inserted before the GFP sequence. The resulting construct rescued the head-bending phenotype of ctn-1 mutants and was used for generating integrated transgenic animals. For GFP: : sgca-1 construct, the GFP sequence was inserted in-frame right after the signal sequence of sgca-1 open-reading frame as described previously [21]. Transgenic strains were made as described [46] by injecting DNA constructs (2–10 ng/µl) along with a co-injection marker DNA (pRF4 (rol-6 (d) ) or ofm-1: : GFP) into the gonad of hermaphrodite animals at 100 ng/µl. We obtained at least 3 independent transgenic lines for rescue, and found that all lines show similar results. To remove bacteria attached to animals, approximately fifteen age-matched (30 hr after L4 stage) hermaphrodite animals for each genotype were placed on a NGM (nematode growth medium) agar plate without bacteria for 15 min. The animals were then placed inside one of two copper rings embedded in a NGM plate. We found that age of agar plate influences the speed of animals, probably because the surface tension resulting from the liquid surrounding animals slows down movement. We used approximately one week-old plates for our assay, and compared with the speed of wild-type control animals. Video frames from two different genotypes were simultaneously acquired with a dissecting microscope equipped with Go-3 digital camera (QImaging) for 2 min with a 500 ms interval and 20 ms exposure. We measured the average speed of animals by using Track Objects from ImagePro Plus (Media Cybernetics). The activity of egg laying muscle was measured indirectly by counting eggs retained in uteri. Single age-matched (30 hrs post-L4) animals (total 15 for each genotype) were placed in each well of a 96 well plate that contains 1% alkaline hypochlorite solution. The eggshells protect embryos from dissolution by alkaline hypochlorite. After 15 min incubation, the remaining eggs were counted in each well. Body curvature analysis was previously described [47]. A single animal was transferred to an agar plate and its movement was recorded at 20 frames per second. We limited image acquisition within 15 to 60 seconds after transfer, because the head bending phenotype is prominent when animals are stimulated to move forward rapidly. A custom-written software automatically recognizes the animal and assigns thirteen points spaced equally from the tip of nose to the tail along the midline of the body, and produces the pixel coordinates of thirteen points. First supplementary angles were calculated from the coordinates of the first three points with MATLAB software. First angle data were obtained when the head swing of an animal reached the maximal extension to the dorsoventral side. Mixed stage worms were washed and collected in M9 buffer. Equal volume of 2× Laemmli sample buffer was added to the worm pellets. The resulting worm suspension was heated at 90 °C for 10 min, centrifuged at 20,000 g for 10 min, and then immediately loaded on 7. 5% SDS-PAGE gel. The Western blot analysis was performed using anti-GFP antibody (Clontech, JL-8) and anti-α-tubulin antibody (Developmental hybridoma bank, AA4. 3). Fixation and immunostaining procedures are previously described [21]. Fluorescence images were observed under a Zeiss Axio Observer microscope with 40× objective (water-immersion, NA: 1. 2) or an Olympus Fluoview 300 confocal microscope with a 60× objective (oil-immersion, NA: 1. 4) or 100× objective (oil-immersion, NA: 1. 4). We typically observed more than 50 animals for each genotype. Images for quantification were acquired under an identical exposure time, gains and pinhole diameter. The intensity of puncta from acquired images was analyzed using linescan (Metamorph, Molecular Devices) and presented as values obtained by subtracting background levels from the peak grey levels of puncta. Electrophysiological methods were as previously described [48]. Briefly, animals raised on 80 µM retinal plates, were immobilized with cyanoacrylic glue and a lateral cuticle incision was made to expose the ventral medial body wall muscles. Muscle recordings were made in the whole-cell voltage-clamp configuration (holding potential −60 mV) using an EPC-10 patch-clamp amplifier and digitized at 2. 9 kHz. The extracellular solution consisted of (in mM): NaCl 150; KCl 5; CaCl2 5; MgCl2 4, glucose 10; sucrose 5; HEPES 15 (pH 7. 3, ∼340mOsm). The patch pipette was filled with (in mM): KCl 120; KOH 20; MgCl2 4; (N-tris[Hydroxymethyl] methyl-2-aminoethane-sulfonic acid) 5; CaCl2 0. 25; Na2ATP 4; sucrose 36; EGTA 5 (pH 7. 2, ∼315mOsm). All of the animals carry a transgene (zxIs6) that expresses channelrhodopsin-2 under the control of the cholinergic motor neuron (unc-17) -specific promoter. Evoked currents were recorded in a body-wall muscle after eliciting neurotransmitter release by a 10 ms illumination using a 470 nm LED (Thor labs) triggered with a TTL pulse from the EPC10 pulse generator [31]. Evoked post-synaptic responses were acquired using Pulse software (HEKA) run on a Dell computer. Subsequent analysis and graphing was performed using Pulsefit (HEKA), Mini analysis (Synaptosoft Inc) and Igor Pro (Wavemetrics). The data were analyzed with one-way ANOVA followed by Dunnett' s multiple comparison.
Calcium ions are essential for many physiological processes, including neurosecretion and neuronal and muscle excitation. Paradoxically, abnormal accumulation of calcium ions is associated with cell death and has been documented as an early event in muscle and neural degenerative diseases. One mechanism to avoid detrimental calcium accumulation is to link the calcium increase with activation of calcium-dependent potassium ion channels, thereby reducing cell excitability and preventing further calcium influx. This negative feedback requires these potassium channels to be localized in close proximity to sites of calcium entry. In a Caenorhabditis elegans genetic screen, we identified α-catulin, known as a cytoskeletal regulatory protein in mammals, important for the localization of calcium-dependent potassium channels in both muscles and neurons. In muscle, α-catulin controls the localization of the dystrophin complex, a multimeric protein complex implicated in muscular dystrophy. The dystrophin complex in turn tethers the calcium-dependent potassium channels near calcium channels. In neurons, the α-catulin-mediated localization of the potassium channels is independent of the dystrophin complex. Lack of α-catulin results in mislocalization of the potassium channels, and in turn causes defects in neuromuscular function. Our results support the idea that cytoskeletal proteins function as anchor molecules that localize ion channels to specific cellular domains.
genetics and genomics/disease models
physiology/cell signaling
physiology/muscle and connective tissue
cell biology/cell signaling
An Alpha-Catulin Homologue Controls Neuromuscular Function through Localization of the Dystrophin Complex and BK Channels in Caenorhabditis elegans