The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography–mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg. kg-1. day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment.
Leishmaniasis is one of the seventeen neglected diseases prioritized by the World Health Organization. Although most cases of neglected diseases are in underdeveloped countries, leishmaniasis is spreading worldwide [1]. The infection caused by Leishmania parasites may remain asymptomatic or evolve to a symptomatic form that can vary from a cutaneous to a visceral form of the disease, the latter of which can be lethal if left untreated [2]. As there is no vaccine against leishmaniasis yet, infected people are treated with antileishmaniasis drugs and control still depends on programs focusing on the vector and reservoir hosts [1,3]. There are a limited number of drugs for the treatment of leishmaniasis and the pentavalent antimonials are the most common [3]. However, antimonials can cause severe adverse effects, such as vomiting, nausea, anorexia, myalgia, abdominal pain, headache, arthralgia, and lethargy, due to their accumulation in the tissues [1]. Until now, efforts to reduce the toxicity of antileishmaniasis drugs have been unsuccessful, which reinforces the need for new antileishmanial drugs. Therefore, protocols that could provide an alternative therapy, reduce dosages, treatment duration and adverse effects for leishmaniasis, would be welcome. Morinda citrifolia Linn. is a small plant native to Southeast Asia. It is commonly known as Noni and is one of the most significant resources of traditional medicine in S. E. Asian countries. The efficacy of Noni in the treatment of pain and inflammatory reactions [4] as well as its antimicrobial activity [5] has been demonstrated in various studies. Recently, morindicone and morinthone, isolated from the stem of M. citrifolia, were shown to have activity, in vitro, against Leishmania (L.) major [6]. In order to demonstrate the antileishmanial activity of M. citrifolia, our group has been using the fruit juice of this plant in in vitro assays with Leishmania (L.) infantum promastigotes and intracellular amastigotes. Our previous results showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity and autophagosome-like vesicles in L. (L.) infantum promastigotes treated with M. citrifolia fruit juice. Cytotoxicity assay with J774. G8 macrophages showed that M. citrifolia fruit juice was not toxic to these cells up to 1000μg. mL-1; however, when intracellular amastigotes were evaluated by light microscopy, macrophages showed vacuoles with probable remains of intracellular parasites [7]. Based on these results, the aim of the present study was to evaluate the antileishmanial activity of M. citrifolia fruit juice under in vivo conditions, using C57BL/6 mice subcutaneously infected with L. (L.) amazonensis.
Morinda citrifolia fruits were collected in São Luiz (S2°31 W44°16), a municipality in the Brazilian Amazon, located 24m above sea level. Fully ripe fruits, with a translucent exocarp, were picked in the rainy season, from April to November 2011. The material was properly identified by Ana Maria Maciel Leite and the voucher specimen number 2000346 was deposited at the Herbarium Professora Rosa Mochel at the Universidade Estadual do Maranhão. Fruits were washed with sterilized distilled water, dried at 25°C and placed in sterile glass bottles for 3 days to drain off the extract. The juice extract, called Noni, from M. citrifolia fruit was centrifuged twice at 4000 rpm for 15 minutes; the supernatant was lyophilized and stored at -20°C. Noni was dissolved in PBS immediately before use in the in vivo experiments. Lyophilized noni was dissolved in methanol to 5mg. mL-1. The LC Shimadzu Nexera UFLC was coupled to an ion trap Bruker Amazon. Analyses were performed at ambient temperature in a 100mm x 2. 1mm x 2. 6μm Kinetex C18 gravity column, equipped with an 8 mm x 4 mm, 5μm guard column. The mobile phase consisted of water containing 0. 1% formic acid (eluent A) and acetonitrile (eluent B). The gradient of B was as follows: in 5. 5 min from 5% to 25%, from 7. 0 to 8. 5 min up to 100% B, held at 100% for 1. 5 min, then 100% to 5% in 1 min, and finally held at 5% for 2 min. The flow rate was 0. 3 mL/min and the injection volume was 1 μL. Other specifications were as described in the literature [8]. Female C57BL/6 mice 4-6-weeks old were obtained from Centro de Criação de Animais de Laboratório (CECAL/FIOCRUZ) and maintained under pathogen-free conditions, controlled temperature and food and water ad libitum. All experiments with animals were conducted in accordance with the guidelines for experimental procedures of the Conselho Nacional de Controle de Experimentação Animal (CONCEA) and approved by Comissão de Ética no Uso de Animais from Fundação Oswaldo Cruz (CEUA-FIOCRUZ), identification number LW72/12. The L. (L.) amazonensis (MHOM/BR/1976/MA-76) obtained from a human case of diffuse infection and characterized by isoenzyme [9] and lectin techniques [10] was maintained in the laboratory by successive passages in BALB/c mice. Prior to infection, parasites were isolated from a non-ulcerated nodular lesion in the footpad and amastigote viability was checked with erythrosine B by light microscopy. 104 amastigote forms were inoculated subcutaneously into the right footpad of C57BL/6 mice. Initially, an 8-week pilot treatment protocol, with two different concentrations of Noni (250 and 500mg. kg-1), was carried out to determine the dose of Noni to be used in the posterior analyses. The daily treatment was carried out with 100μL of Noni by gavage. A group of non-treated infected mice was maintained as control. Lesion thickness was evaluated weekly in order to choose the most efficient drug concentration. Treatment protocol was performed with 5 groups of 10 animals, as follows: infected and treated (100μL of Noni 500mg. kg-1 by gavage, daily); infected and control drug-treated (Glucantime 20mg. kg-1 by intramuscular injection, twice a week); infected and mock-treated (100μL of PBS by gavage, daily); mock-infected and treated (100μL of Noni 500mg. kg-1 by gavage, daily); and normal (mock-infected and mock-treated). Treatment started 55 days after infection for all groups. Lesion kinetics was evaluated weekly by a caliper rule, in comparison to the non-infected contralateral footpad and expressed as lesion thickness. After 30 and 60 days of treatment animals were euthanized, blood was collected to obtain serum and tissue fragments from footpad, draining lymph nodes and liver were excised for posterior analyses. DNA from the footpad and draining lymph nodes of 3 animals per group was extracted following a standard phenol/chloroform protocol [11]. DNA concentration was quantified in a NanoDrop 2000c spectrophotometer (ThermoScientific). Parasite load was estimated by real time PCR performed in Applied Biosystems Step One Plus equipment, using Fast SYBR Green Master Mix. Primers were target for the parasite kDNA and mouse β-actin was used as an endogenous control (S1 Table). Skin, lymph nodes and liver fragments were fixed in 10% buffered formalin and routinely processed for paraffin embedding. Tissue sections (5μm thick) were stained with Hematoxylin-Eosin, Gomori trichrome and Picrosirius red. Tissues were observed under a light microscope and polarized light was used to observe the collagen fibers. After euthanasia, skin fragments of infected footpads from 3 mice of each group were collected. Total RNA was extracted using TRIZOL reagent (Invitrogen, Karlsruhe, Germany) following the manufacturer’s instructions. cDNA synthesis was performed with 1μg of total RNA using a iScript cDNA Synthesis kit (Bio-Rad Laboratories, Hercules, CA) according to the manufacturer’s recommendations. Primers targeting the genes IL-4, IL-10, IL-12, TNF-α, IFN-γ, TGF-β, iNOS, Laminin, Fibronectin and Collagens I, III and IV were designed using the Primer Express software version 3. 0 (Applied Biosystems, 2004), and manufactured by Invitrogen (Supplementary Data 1). Real Time PCR assays were performed using Power SYBR Green Master Mix and the relative quantification (2-ΔΔCT) method was applied, using the mouse RPLP0 gene (large ribosomal protein, P0) as the endogenous control. Results were analyzed with the StepOne Software v2. 3 (Applied Biosystems). A pool of sera obtained from the blood of five mice per group was used for cytokine quantification of IL-4, IL-10, IL-12, TNF-α, IFNγ (BD Bioscience) and TGF-β (R&D System) following the manufacturer' s specifications. Clinical signs of toxicity, such as piloerection, diarrhea, salivation, convulsions or changes in mobility, respiration rate or muscle tone, were observed during the treatments. Levels of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein, direct bilirubin, indirect bilirubin, total bilirubin, albumin, globulin, urea and creatinine were analyzed in sera pools from mice treated for 60 days in Ciba Corning equipment. At necropsies, stomach and gut mucosa were macroscopically evaluated for abnormal findings. Animal weight was measured on an analytical balance after 30 and 60 days of treatment. The values were expressed as mean ± S. D. The results were analyzed statistically by Analysis of Variance (ANOVA) followed by Bonferroni’s post-test. The analyses were performed with the software GraphPad Prism 5. 0. 4. Differences were considered significant when p<0. 05.
According to the selective ions and elution order obtained from the Liquid Chromatography–Mass spectrometry analysis and compared with references in the literature [8], five compounds were identified: deacetylasperulosidic acid (1), asperulosidic acid (2), rutin (3), nonioside B (4) and nonioside C (5) (Fig 1). The extract ion chromatograms (m/z) of these compounds were respectively 389,431,609,629 and 467. The pilot protocol showed that Noni at 500mg. kg-1 could significantly reduce lesion growth from the fourth week of treatment. Therefore, the dosage of 500mg. kg-1 was chosen for subsequent protocols. In this protocol, the treatment was able to significantly reduce lesion size as of the sixth week, when compared with the infected non-treated group (Fig 2A). The control drug, Glucantime 20mg. kg-1, was also able to decrease lesion size, showing no statistical difference with Noni treatment. After 30 days of Noni treatment there was no change but after 60 days the parasite loads in the footpad and draining lymph node had significantly decreased in comparison to the non-treated control, corroborating with the results of the lesion kinetics (Fig 2B and 2C). On the other hand, Glucantime was able to reduce parasite loads after 30 days of treatment for the footpad and after 60 days for the lymph node. Histopathological analysis of the lesion site of mock-treated mice showed inflammatory infiltrates composed of parasitized macrophages 30 and 60 days after infection (Fig 3). In the former, the infiltrated area had increased and the number of infected macrophages enhanced. Furthermore, a large area of necrosis and lesion ulcerations was observed. Also at 30 days after infection the draining lymph node presented hyperplasia of the cortical region. Noni treatment at 30 and 60 days reduced the parasite loads and inflammatory infiltrate. Remarkable tissue remodeling at the lesion site and depletion of the number of blast cells in the lymph node were observed after 60 days of treatment. Also, at that time, no parasites were found in the Glucantime-treated mice at the lesion site. A reduction of the inflammatory infiltrate was also noted in the skin as well as the reestablishment of the normal histopathological pattern of the lymph node. When the expressions of IFN-γ, iNOS, IL-12, TNF-α, IL-10, TGF-β and IL-4 at the lesion site were evaluated, no difference was noted between Noni and mock-treated non-infected mice (Fig 4). In mock treated infected mice, IFN-γ and iNOS showed upregulation after 30 days of treatment but this was not observed on the 60th day. In Noni treated infected mice this upregulation was not observed and mice showed the normal value throughout the experiment. However, Glucantime treated infected mice presented an upregulation of these cytokines principally on the 30th day of treatment. An upregulation of IL-4 was also noted at this time in Glucantime and mock treated infected mice. The IL-12, TNF-α and IL-10 expressions were upregulated in mock treated infected mice especially after the 60th day. This upregulation was not observed in infected and Noni or Glucantime treated mice. TGF-β was upregulated throughout the experiment in mock treated infected mice. However, TGF-β was upregulated only after 60 days of treatment in Noni or Glucantime treated mice. Cytokine levels in the serum showed that L. (L.) amazonensis increased IL-4 and TNF-α at 30 days and IL-10 at both treatment times (Fig 5). This high production of TNF-α was also observed in the infected groups treated with Noni or Glucantime. IL-10 showed a lower increase in infected and treated groups. On the other hand, IL-4 production decreased in treated groups whether infected or not. No alterations were observed in IL-12 or TGF-β except for a slight increase in TGF-β production for the Noni treated infected mice. Finally, Glucantime treatment increased IFN production after 60 days. Observation of the lesion site skin stained with hematoxylin-eosin showed a reduction in the normal structure of the dermis and a degradation of the connective tissue in infected footpads when compared with mock-infected groups. This difference in the presence of collagen fibers among the groups was demonstrated using Gomori´s trichrome and Picrosirius Red (Fig 6A). To quantify these alterations, the extracellular matrix protein expression was evaluated by qPCR in footpad skin after 60 days of treatment (Fig 6B–6F). Noni fruit juice upregulated the expression of all analyzed proteins, except for collagen IV in mock-infected mice; whereas L. (L.) amazonensis downregulated the expression of fibronectin, collagen I and IV when compared with normal mice. Noni and Glucantime treatment preserved the normal expression of collagen IV, laminin and fibronectin in infected footpads but they decreased collagen I and III expression. No clinical signs of toxicity were observed during the treatments and there was no mortality. There was no significant statistical alteration in the weight of the animals after 30 and 60 days of treatment. During necropsy, alterations such as hyperemia were not observed in the stomach or gut mucosa of the animals treated with Noni. Also there was no change in the sera biochemical parameters of hepatic and renal functions, except for alanine transaminase (ALT). Infection and Noni treatment enhanced ALT levels but within the normal maximum limit. Histopathology showed that Noni treatment in mock-infected mice did not stimulate an inflammatory reaction in the liver (Fig 7). The L. (L.) amazonensis on the contrary, induced a diffuse and periportal inflammatory infiltration, the latter being reduced by Noni or Glucantime treatments.
M. citrifolia has various biological actions including leishmanicidal [6] and immunomodulatory activities [12,13] that have not yet been fully elucidated. The chromatographic analysis of the Noni juice used in our studies showed the same pattern of other Noni juices produced around the world [8]. It is translucent and brown; presents medium viscosity, characteristic odor, pH 3. 9 and yielded 6. 31% of a highly hygroscopic powder [7]. In this study we used Noni juice to treat C57BL/6 mice infected with L. (L.) amazonensis. We chose to begin the treatment 55 days after infection, when the lesion was well established, in order to better mimic treatment in humans. In fact, when treatment began, all lesions were about 2mm thick. Noni treatment decreased the lesion size associated with a lower parasite load in the skin and draining lymph nodes after 60 days of treatment. Treatment with the control drug, Glucantime, caused a faster reduction of the parasite load than Noni. However, after 60 days of treatment, Noni had reduced the lesion size more than Glucantime. The lesion size reduction after Noni treatment is associated with a decreased parasite load and control of the inflammatory process caused by L. (L.) amazonensis. The histopathology and cytokine expression analysis showed a reduction in focal inflammation in the skin after Noni treatment with a downregulation of cytokine expressions (IL-12 and TNF-α) at 30 and 60 days of treatment. IFN-γ plays a crucial function in controlling the Leishmania infection, as has been demonstrated in mice with genetic defects in this molecule and/or its receptor [14]. IFN-γ induces parasite elimination by activating both phagocyte oxidase (phox) and iNOS, which is the most effective mechanism of killing intracellular parasites mediated by macrophages [15,16]. In vitro, our group demonstrated an increase of nitric oxide production and iNOS expression in the peritoneal macrophages infected with L. (L.) amazonensis and treated with Noni [17]. In the present study, the association of high levels of IFN-γ and iNOS and decrease of parasite load was observed in Glucantime treatment, but not in Noni treated mice, suggesting a different mechanism of parasite killing in vivo. Our results demonstrated that normal levels of IL-10 expression in treated groups were associated to low parasite burden, while high levels of IL-10 expression were associated to elevated parasite burden in mock-treated infected mice showing the role of IL-10 in maintaining the infection. The same results have been described in IL-10 knock-out mice which were more competent in controlling L. (L.) major infection than cells from wild type mice [18]. The TGF-β expression was also upregulated in infected mice. Proteins secreted by infected macrophages or the promastigote forms of L. (L.) infantum chagasi activate the soluble form of latent TGF-β complex favoring the persistence of parasites within infected macrophages through induction of TGF-β mediated anti-inflammatory mechanisms [19]. The role of TGF-β as a key predictive factor of enhanced susceptibility to the disease was also demonstrated in BALB/c mice immunized with whole antigens of L. (L.) amazonensis. Species-specific components of vaccine activate TGF-β production that predisposes more susceptible individuals to a more aggravated form of the disease [20]. Thus, a low TGF-β expression in Noni treated and infected mice contributes to maintain the control of inflammatory infiltrates when compared with infected mock-treated mice. The phenotype of susceptibility in L. (L.) major infection is clearly associated to high levels of IL-4 and Th2 response [21]. IL-4 reduces iNOS expression and enhances disease progression due to increased survival and growth of Leishmania parasites in infected cells [22]. In our work, L. (L.) amazonensis enhanced the IL-4 expression as expected, while treatment with Noni maintained lower levels of IL-4 expression in infected mice. As the treatment was performed by gavage, the amount of cytokines in sera allows us to verify the immunomodulatory effect of Noni. In addition, L. (L.) amazonensis infection is not limited to the skin. The parasite tends to disseminate to the lymph nodes and can even reach the spleen and liver [23]. The cytokines measured in sera revealed an enhancement of IL-4 and IL-10 caused by L. (L.) amazonensis infection, which were not seen after Noni or Glucantime treatment. The decreased levels of IL-4 and IL-10 contribute to maintain a Th1 response in the treated groups. Furthermore, the increase of IFN-γ levels at 60 days due to Glucantime treatment contributes to the effectiveness of the macrophages by iNOS induction in skin and parasite load decrease. Indeed, Noni treatment decreased the IL-4 levels even in mock-infected mice; this may be due to the activation of cannabinoid 2 receptors [12]. Altogether, these results endorse the immunomodulatory effects of Noni. Studies have reported that an immunochemotherapy is more effective than chemotherapy or immunotherapy [24], and our data show that Noni treatment is actually immunochemotherapy. In addition to cytokine modulation, the skin histopathology analysis showed that Noni helps to control the inflammatory infiltrates and supports an early remodeling process. The tissue repair process is critically important for rapid cure of cutaneous leishmaniasis, as demonstrated in L. (L.) major murine cutaneous leishmaniasis [25], and is associated to reduced IL-10 and increased TNF-α, IFN-γ [26] and the TGF-β pathway [27]. In a non-infected wound, high levels of IL-10 decrease pro-inflammatory mediators and inflammation, normal collagen deposition and restoration of normal dermal architecture [28], whilst TGF-β induces immune cell recruitment, promotes matrix protein synthesis while decreasing matrix protein degradation leading to fibrotic tissue formation [29]. In contrast, Noni treatment promotes a control of the inflammatory process which contributes to a favorable ambient for tissue repair. The increase in TGF-β levels after 60 days of treatment with Noni, when compared to 30 days of treatment, may be associated with this tissue repair. The excessive secretion of pro-inflammatory cytokines and chemokines, as observed in mock-treated infected mice, can recruit and activate additional inflammatory cells and lead to uncontrolled tissue degradation, including new granulation tissue and growth factors, delaying collagen deposition, which impairs the repair process and perpetuates the non-healing condition [30]. The histopathological evaluation of collagen fibers and protein expression in the skin confirmed a modulation of extracellular matrix proteins in Noni-treated mock-infected mice. Anthraquinones were previously identified in our Noni juice [7] and an anthraquinone isolated from Noni fruit has been shown to stimulate collagen type I, the major component of extracellular matrix of the skin in human dermal fibroblasts. Nano-emulsion with this anthraquinone increased the dermal procollagen I in nude mouse skin [31] in the same way as the Noni increased collagen I expression in mock-infected mice. Moreover, the overexpression of collagen III, laminin and fibronectin by Noni treatment is reported here for the first time. The extracellular matrix protein expressions most affected by Leishmania infection were collagen I, collagen IV and fibronectin. The role of collagen I, IV and fibronectin during Leishmania infection have been well described in the literature. L. (L.) mexicana binds fibronectin and collagen I to promote adhesion and phagocytosis by macrophages [32,33]. Degradation of fibronectin and collagen IV by glycoprotein gp63 seems to enhance L. (L.) amazonensis migration. Leishmania-degraded fibronectin by surface and secreted leishmanolysin also decreases the production of reactive oxygen intermediates by parasite-infected macrophages and affects the accumulation of intracellular parasites [34,35]. Treatment with Noni or Glucantime restored the collagen IV and fibronectin expressions to normal levels. This is possibly due to the reduction of parasitic burden and control of the inflammation process with Noni treatment. In addition, Noni treatment also caused an upregulation of laminin expression, a protein related to the degradation and binding of Leishmania [36]. Finally, the toxicity parameters analyzed in our model indicated that Noni treatment has no toxic effect on mice. No alterations in the mucosa of stomach or gut were found, showing that the Noni juice does not irritate the digestive system. This result was expected since a previous work described that M. citrifolia had a preventive effect on gastro-esophageal inflammatory diseases [37]. Although there was a slight increase in ALT, which did not exceed the normal limits, there was a decrease in the hepatic inflammation caused by L. (L.) amazonensis. Nevertheless, Noni toxicity still needs more studies, considering the controversial data in literature that sometimes show toxicity [38,39], no toxicity [40–42] or even a liver protective effect [43]. The present work has proved the efficacy of Noni juice in reducing the parasite burden and lesion size. In addition, it has shown its modulatory effects on cytokine and extracellular matrix protein expressions. Altogether, Noni treatment has an antileishmanial activity, associated with an immunomodulatory action, which opens a new path to follow in the quest to promote a rapid clinical cure of cutaneous leishmaniasis.
Leishmaniasis is a complex of diseases caused by parasites of the Leishmania genus, which affects thousands of people around the world. The parasite lives within the cells and the disease manifests itself in different ways, one of them is wound-like lesions on the skin that do not heal. The treatment, with a medicament discovered in 1912, causes several side effects, its intramuscular administration is painful and it is given daily over a long period of time. These characteristics show the need for new alternatives for leishmaniasis treatment justifying works like this. The Morinda citrifolia is a plant native to the Polynesian islands and has a fruit commonly known as Noni. Noni has been analyzed for various targets such as anticancer, anti-inflammatory and antimicrobial effects; however, the antileishmanial has not yet been fully evaluated. This work proves that Noni treatment can promote a fast clinical cure in mice with leishmaniasis by decreasing parasite number, acting on the immune system and repairing skin components.
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Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis