While the importance of gene enhancers in transcriptional regulation is well established, the mechanisms and the protein factors that determine enhancers activity have only recently begun to be unravelled. Recent studies have shown that progesterone receptor (PR) binds regions that display typical features of gene enhancers. Here, we show by ChIP-seq experiments that the chromatin remodeler CHD8 mostly binds promoters under proliferation conditions. However, upon progestin stimulation, CHD8 re-localizes to PR enhancers also enriched in p300 and H3K4me1. Consistently, CHD8 depletion severely impairs progestin-dependent gene regulation. CHD8 binding is PR-dependent but independent of the pioneering factor FOXA1. The SWI/SNF chromatin-remodelling complex is required for PR-dependent gene activation. Interestingly, we show that CHD8 interacts with the SWI/SNF complex and that depletion of BRG1 and BRM, the ATPases of SWI/SNF complex, impairs CHD8 recruitment. We also show that CHD8 is not required for H3K27 acetylation, but contributes to increase accessibility of the enhancer to DNaseI. Furthermore, CHD8 was required for RNAPII recruiting to the enhancers and for transcription of enhancer-derived RNAs (eRNAs). Taken together our data demonstrate that CHD8 is involved in late stages of PR enhancers activation.
During the last decade it has become clear that regulation of gene transcription is accompanied by extensive changes in the chromatin organization of promoters [1]. More recently, efforts have concentrated on elucidating the chromatin dynamics of distal regulatory regions and particularly enhancers [2,3, 4]. Enhancers were originally defined as regulatory sequences that can activate gene expression independently of their proximity or orientation with respect to their target genes [5]. Even though histone modifications signatures (e. g. , high levels of histone H3 lysine 4 monomethyl (H3K4me1) and H3K27 acetyl (H3K27ac) modifications) have provided insight for the discovery of enhancer-like regions, the mechanisms and the factors that control enhancers activation are not yet well known. Chromatin changes are normally performed by two types of enzymes: enzymes that chemically modify histones or ATP-dependent chromatin remodelers. ATP-dependent remodelling is performed by enzymes of the SNF2 family that use the energy of ATP hydrolysis to destabilize the interaction between DNA and histones [6,7]. In humans there are 26 ATPases of this family with specific roles in gene transcription and in other aspects of DNA metabolism. One of these ATPases is CHD8 which, in addition to the ATPase domain, contains two chromodomains in the amino terminus of the protein, and two BRK domains in the carboxy terminus [8]. CHD8 is able to remodel nucleosomes in vitro in an ATP-dependent reaction [9]; however, its in vivo functions are unclear. Inactivation of CHD8 by homologous recombination in mice provokes a strong growth retardation from embryonic day 5. 5 and developmental arrest accompanied by massive apoptosis [10]. Ishihara et al. reported that CHD8 interacts with CTCF and plays a role in insulation activity [11]. It has also been reported that CHD8 represses beta-catenin target genes, and suppresses p53-dependent activation and apoptosis, by promoting histone H1 recruitment [9,12,13,14]. A role in repression of MLH1 gene, associated to MAFG, has been also shown [15]. In contrast to this repressive role, we have shown that CHD8 is required for E2F-dependent activation of G1/S specific promoters [16,17]. Additionally, it has been reported that CHD8 is required for estrogen-dependent induction of Cyclin E2 gene [18], and for recruitment of androgen receptor (AR) and activation of the TMPRSS2 gene [19]. These two studies indicate that CHD8 is also involved in steroid hormone-dependent transcriptional regulation although the mechanisms of this regulation are unknown. In this work, we have investigated the role of CHD8 in progesterone-dependent transcriptional regulation. Progesterone controls transcription through a complex mechanism [20]. On the one hand, progesterone-bound progesterone receptor (PR) is able to bind specific DNA sequences in chromatin, called PRE, and to recruit histone modification enzymes and ATP-dependent chromatin remodelers, such as NURF and BAF complexes (a member of the SWI/SNF complex family) [21,22]. On the other hand, a small fraction of PR is attached to the cytoplasmic side of the cell membrane and, in the presence of hormone, interacts with tyrosine kinases provoking the activation of various kinase cascades, including ERK1/ERK2 [23,24]. Activated ERK1/ERK2 phosphorylates PR and the kinase MSK1, forming a ternary complex that binds to chromatin. Recent genome-wide studies demonstrated that most PR binding sites (PRbs) are distal regulatory regions that map at introns and intergenic regions and that display typical histone modifications of enhancer regions [25,26,27]. Here, we demonstrate that CHD8 is required for the activation of PR enhancers. In proliferating T47D breast carcinoma cells, CHD8 is mostly associated with promoters. However, upon progesterone treatment, CHD8 was quickly recruited to a subset of transcriptionally competent PR enhancers. In agreement with these data, depletion of CHD8 strongly impaired progesterone-regulated gene expression. CHD8 recruitment to the enhancers was dependent of PR but independent of the pioneering factor FOXA1. Interestingly, depletion of the SWI/SNF complex ATPases, BRG1 and BRM, impaired CHD8 recruitment. Furthermore, we observed that CHD8 interacts with the SWI/SNF complex. CHD8 was not required for H3K27 acetylation, but depletion of CHD8 impaired hormone-dependent RNAPII recruitment at enhancers and synthesis of enhancer RNAs (eRNAs), suggesting that CHD8 is required for enhancer transcription.
To identify the genome-wide distribution of CHD8 in proliferating human breast cancer cells T47D-MTVL [28], we performed chromatin immunoprecipitation of CHD8 followed by deep sequencing (ChIP-seq). We found 12655,4900 and 2500 peaks of CHD8 by using three different confidence threshold values, respectively (ChIPseeqer threshold level 10–10,10–15 and 10–20). In all cases, false discovery rates (FDR) were lower than 0. 03 (see Materials and Methods). At the lowest threshold about 48% of the peaks (6257) were located within promoters, with a strong enrichment around TSS (Fig 1A and 1B) and about 50% of the peaks were distributed between introns (2187) and intergenic regions (3748). Interestingly, if confidence threshold for peaks identification is increased, the percentage of peaks associated with promoters raise to about 78% (Fig 1A). This is due to differences in CHD8 signal intensity. In fact, CHD8 signals at TSSs are higher than at intergenic or intronic regions (Fig 1C and 1D). Therefore, when only highly significant peaks are analyzed most of them map at promoters. Consistently, a strong overlap between CHD8 and RNA polymerase II (RNAPII) or H3K4me3 ChIP-seq signals was observed (Fig 1C and 1E). CHD8 occupancy was confirmed by ChIP-qPCR in three selected target promoters (CCND1, HDS11B2 and CCNE2) using a different anti-CHD8 antibody (S1A Fig). Furthermore, as control, we also verified that knockdown of CHD8 decreased ChIP-qPCR signal (S1B Fig). All these experiments validated our ChIP-seq results. The rest of the analysis was performed using the 12655 CHD8 sites identified at the lowest, but still very significant, threshold. We have previously reported that CHD8 binds 1965 promoters in a ChIP-on-chip analysis of proliferating cervical carcinoma C33 cells [17]. Approximately 60% of the promoters identified as CHD8 targets by ChIP-on-chip (1175) were also identified by ChIP-seq, despite the different cell lines used. CHD8 bound genes were enriched in Gene Ontology categories related to macromolecular biosynthetic processes (transcription, mRNA processing) and cell cycle (S2A Fig). Consistently with our previous data from C33 cells [17] CHD8 target promoters were strongly enriched in E2F (p-value = 3. 6 × 10–135), ELK-1 (p-value = 2. 2 × 10–122), AP-2 (p-value = 4. 7 × 10–100), and SP1 (p-value = 4. 2 × 10–80) transcription factors binding sites (S2B Fig). Taken together these results indicate that CHD8 is mostly bound to promoters in proliferating T47D-MTVL cells. CHD8 has been suggested to be a nuclear receptor co-activator [18,19]; however, very little is known about this function of the protein. To gain insight into its role in hormone dependent transcriptional regulation we have analyzed by ChIP-seq the distribution of CHD8 in progesterone-treated T47D-MTVL cells. For that, cells were subjected to 48 h of serum deprivation and then stimulated during 5 or 45 minutes with the synthetic progestin R5020 (10 nM) or the vehicle (ethanol) as control. A very small number of peaks were found in vehicle treated cells, suggesting that serum deprivation strongly decreases the association of CHD8 to the chromatin. These data extend our previous observation about absence of CHD8 in four G1/S transition genes in quiescent cells [17]. However, 1132 and 4532 progestin-induced CHD8 peaks were found at 5 and 45 minutes, respectively, suggesting that CHD8 is quickly recruited to the chromatin after progestin treatment. Most of the sites found after 5 minutes (73%) were also identified after 45 minutes (Fig 2A). Only 18% (832) of the hormone-specific peaks were also found under proliferating conditions (Fig 2A). A large majority of the hormone specific sites were found at intronic and intergenic regions (Fig 2B) and low enrichment was found at TSS (S3 Fig). A representative example of the ChIP-seq data close to four well-known progesterone-dependent genes (HSD11B2, FKBP5, NFE2L3 and IL6ST) is shown in Fig 2C. De novo analysis of sequence motifs in progestin-dependent CHD8 bound regions identified a highly significant enrichment (p-value = 4. 7 × 10–75) for the sequence CTGTNC, which is very similar to the consensus sequence of the progesterone receptor binding site TGTYCY [25] (Fig 2D). PR binds 24436 sites in T47D-MTVL cells 60 min after progestin treatment [25]. Interestingly, 83. 2% of the CHD8 progestin-dependent peaks (3770) co-localized with PR peaks, indicating that CHD8 is recruited to a subset of PRbs upon hormone induction (Fig 2E). Thus, CHD8 was significantly enriched around PRbs (Fig 2F). Ballaré et al. have recently reported that PRbs present high nucleosome occupancy and that functional PR sites, involved in transcription control, display a high nucleosome-remodelling index (NRI) [25]. NRI is the ratio between the nucleosome occupancy before and after hormone administration. Strikingly, we found strong CHD8 occupancy at PRbs with high NRI (top 10% higher NRI) and weak CHD8 enrichment at PRbs with low NRI (top 10% lower NRI), suggesting that CHD8 is associated with functional PRbs, where a strong nucleosome remodelling is occurring (Fig 2G and 2H). Similarly to CHD8 binding sites, PRbs are mostly found in introns and intergenic regions [25]. Distal regulatory regions and enhancers are enriched in the histone acetyltransferase p300 [29]. In the presence of R5020, CHD8 signal was strongly enriched around p300 binding sites (Fig 2I), indicating that CHD8 binding sites display enhancer characteristics. Another typical enhancer feature is the presence of monomethylated histone H3 lysine 4 (H3K4me1) [30]. CHD8 was moderately enriched in all regions with high H3K4me1 (S4 Fig). Most interestingly, we observed strong enrichment of CHD8 around H3K4me1 containing PRbs (Fig 2J). Taken together all these data indicate that CHD8 binds functional PR enhancers in a hormone-dependent manner. It has been reported that CHD8 interacts and cooperates with the insulator factor CTCF (CCCTC-binding factor) [11]. Therefore, we have studied the co-localization of both factors under normal growth conditions or after progesterone stimulation. In proliferating T47D-MTVL cells about 16. 5% (p-value = 6. 0 x 10–130, hypergeometric distribution) of the CHD8 containing regions were also enriched in CTCF (S5A Fig). However, this was only 4. 4% of the CTCF sites. CTCF mostly binds to intergenic or intronic regions [31]. Consistently, most of the CTCF-CHD8 co-occupied sites (66%) were also at intergenic and intronic regions (S5B Fig). Upon progesterone stimulation only about 5. 4% of the CHD8 sites and 0. 6% of the CTCF sites are co-occupied by both factors (S5C Fig), suggesting that CHD8 and CTCF do not cooperate for progesterone-mediated regulation. To correlate CHD8 binding sites with CHD8-regulated gene expression we performed a transcriptomic analysis of T47D-MTVL cells transfected with a control siRNA or a siRNA specifically targeting CHD8 and stimulated during 6 h with progestin or vehicle (Fig 3A and 3B). We found 1170 genes differentially expressed (FDR< 0. 01 and lineal change > 1. 5 fold) after progestin treatment of control cells, of which 793 were up-regulated and 377 down-regulated. About 52. 5% of these genes (614) were misregulated in CHD8-depleted cells with respect to control cells. Interestingly, CHD8-dependent genes presented lower induction of up-regulated genes and lower repression of down-regulated genes, indicating that CHD8 is required for progesterone-dependent regulation of a subset of genes (Fig 3C). Consistently, around 42% of the CHD8-dependent genes (257) were found to be close to CHD8 genomic locations (P = 9. 96 x 10–103) (Fig 3D). Next, we verified by RT-qPCR that CHD8 was required for normal progestin-dependent induction of several well known progesterone dependent genes that contain close hormone-dependent CHD8 binding sites, such as HSD11B2, DUSP1, FKBP5, NFE2L3 and IL6ST genes. Thus, depletion of CHD8 severely impaired accumulation of mRNA, both 45 min and 6 h after progestin treatment (Fig 3E). As a control, we confirmed that a different siRNA that targets CHD8 had similar effects on expression of progesterone dependent genes (S6 Fig). T47D-MTVL cells contain a single copy of the MMTV-Luc transgene integrated in their genome [28]. CHD8 was also strongly enriched in a progestin-dependent manner at the MMTV-Luc transgene promoter (Fig 3F). Furthermore, depletion of CHD8 severely impaired induction of MMTV-Luc (Fig 3E and S6 Fig). In summary, these data demonstrate that CHD8 is necessary for progestin-dependent regulation, at least in a subset of target genes. Next we selected four CHD8 peaks close to HSD11B2, FKBP5, NFE2L3 and IL6ST genes that display high ChIP-seq enrichment for CHD8 and PR upon progestin stimulation (Fig 4A). These regions were also enriched for the typical enhancer factor p300 (Fig 4A). Therefore, we called these regions HSD11B2e, FKBP5e, NFE2L3e and IL6STe. All enhancers were located upstream of the corresponding genes (Fig 2C). Then, we decided to investigate how PR affects CHD8 recruitment to these enhancers and vice versa. First we analyzed PR and CHD8 recruitment to CHD8 progestin-dependent peaks in T47D-MTVL cells or in T47D-YV, a PR-negative clonal derivative cell line of T47D [32] (Fig 4B). We verified by western blotting that levels of CHD8 were identical in T47D-MTVL and in T47D-YV (Fig 4B). ChIP experiments performed 45 minutes after progestin stimulation confirmed that high levels of PR are recruited to all analyzed regions in T47D cells but, as expected, not in T47D-YV cells (Fig 4C). Interestingly, CHD8 was also strongly recruited to all analyzed regions in T47D-MTVL cells but not in T47D-YV cells, suggesting that PR is required for hormone-dependent CHD8 recruitment to PRbs (Fig 4C). A significant recruitment of CHD8 to the IL6ST regulatory region was observed in T47D-YV cells suggesting that CHD8 is recruited to this region, at least in part, independently of PR. Residual binding of progestin to other nuclear receptor might be responsible of this effect. Next, we determined whether CHD8 is involved in PR recruitment to PRbs in T47D-MTVL cells. Fig 4D shows that CHD8 depletion (siCHD8) did not affect progestin-dependent PR recruitment to the four analyzed regulatory regions. As a control, we verified that silencing of CHD8 strongly decreased its association with chromatin, validating the ChIP signals. Taken together, these data indicate that PR is required for CHD8 recruitment to progesterone-dependent CHD8 binding sites, but PR does not require CHD8 for binding. FOXA1 is a fork-head family transcription factor able to directly bind to DNA in the surface of a nucleosome [33]. Because of this pioneering ability, FOXA1 is able to facilitate estrogen receptor binding to the chromatin of hormone-dependent enhancers [34,35]. Since PR is able to bind directly to nucleosomes [28], it is unclear whether FOXA1 also cooperates with PR. ChIP-seq data of FOXA1 distribution in unstimulated T47D cells are available from ENCODE. Using these data we have observed that 52% (2358) of the CHD8 binding sites were also enriched for FOXA1 (Fig 5A). Furthermore, 54% (2022) of the CHD8-PR co-occupied regions were also occupied by FOXA1. Given this very significant enrichment (P = 1. 17 x 10–173) we decided to study the role of FOXA1 in CHD8 and PR recruiting. First, we investigated using ChIP whether FOXA1 binds to the analyzed regulatory regions of HSD11B2, FKBP5, NFE2L3 and IL6ST genes. As shown in Fig 5B significant levels of FOXA1 were found at all studied regions. FOXA1 occupancy after progestin treatment at HSD11B2e, NFE2L3e and IL6STe enhancers was similar in T47D-MTVL and T47D-YV cells, suggesting that PR is not involved in FOXA1 recruitment at these regions (Fig 5B). However, a possible role of ligand-bound PR in FOXA1 recruitment was observed at the FKBP5e enhancer (Fig 5B). Next, we determined the effect of FOXA1 silencing (Fig 5C) on the hormone-dependent recruitment of CHD8 and PR in T47D-MTVL cells. Surprisingly, depletion of FOXA1 significantly increased the level of occupancy of both PR and CHD8 at the four regulatory regions analysed (Fig 5D and 5E), suggesting that, at least in these progestin-dependent regulatory regions, FOXA1 does not help PR and subsequent CHD8 recruitment. Moreover, our data suggest that FOXA1 might compete with PR for binding to PR-enhancers. PBAF and BAF are closely related chromatin remodelling complexes of the SWI/SNF family, which share multiple protein subunits. The BAF complex is required for progesterone-dependent gene activation [21,22,25]. Furthermore, BAF250 and BAF57, two of the subunits of the complex, interact with PR in a hormone-dependent manner [22]. Then, we decided to investigate whether CHD8 interacts with the SWI/SNF complexes. For that, we performed immunoprecipitation using anti-CHD8 antibodies from extracts of T47D-MTVL cells. CHD8 co-precipitated with the core subunits INI1/hSNF5/BAF47, BAF170 and BAF155 and with the ATPase BRG1 in T47D-MTVL cells (Fig 6A). All these subunits form part of both SWI/SNF complexes: BAF and PBAF. To identify the type of SWI/SNF complex interacting with CHD8, we also investigated the presence of BAF180, a PBAF-specific subunit, and BAF250, a BAF-specific subunit. As shown in Fig 6A both subunits co-precipitated with CHD8 indicating that CHD8 can interact with both complexes. The CHD8-SWI/SNF interaction was found both, in the presence and in the absence of hormone (S7A Fig). Next, we studied whether the SWI/SNF complex is required for CHD8 recruitment to PRbs. First, we verified by ChIP that BAF155, one of the core subunits of the complex, is recruited to the four analyzed CHD8-bound enhancers and to the MMTV promoter (Fig 6B). Then, we demonstrated that knockdown of BRG1 and BRM (S7B and S7C Fig) significantly impaired recruitment of CHD8 to the four analyzed PR enhancers and to the MMTV promoter (Fig 6C). Taken together, these data suggest that CHD8 interacts with the SWI/SNF complex and that this interaction contributes to recruit or to stabilize CHD8 at PRbs. Presence of H3K27Ac distinguishes active enhancer states from those poised for activation [36,37,38]. H3K27 is acetylated by p300 [39] and we have shown that CHD8 is enriched around p300 binding sites (Fig 2I). To further characterize the role of CHD8 in enhancer activation we investigated whether CHD8 affects the level of H3K27Ac at the four selected CHD8 binding sites. At the FKBP5e and IL6STe regions H3K27 acetylation was enhanced by R5020 (Fig 7A). However, significant levels of H3K27Ac were already observed under un-stimulated conditions at the HSD11B2e and NFE2L3e enhancers, which were not further stimulated by progestin (Fig 7A). Interestingly, CHD8 depletion did not affect the level of H3K27Ac at any of the analyzed regions, suggesting that CHD8 is not involved in p300 recruiting or H3K27 acetylation. Next we investigated whether CHD8 is required to open the chromatin of its target enhancers. For that, we performed quantitative DNase I sensitivity assays in HSD11B2e, FKBP5e, NFE2L3e and IL6STe enhancer regions in the presence of hormone or vehicle, as described in [40]. Interestingly, CHD8 knockdown decreased hormone-dependent accessibility to the enzyme of all the analyzed regions suggesting that CHD8 is involved in the hormone-dependent chromatin remodelling of these regions (Fig 7B). Several studies have shown that many active enhancers present significant occupancy of RNAPII and regulated production of bidirectional RNA, called eRNAs [41,42,43,44,45]. We have investigated whether RNAPII is recruited to FKBP5e, NFE2L3e and IL6STe enhancer regions and the order of recruitment with respect to PR and CHD8. For that, binding of PR, CHD8 and RNAPII at 0,2, 5,15 and 30 min after progestin stimulation was determined by ChIP-qPCR (Fig 8A). As previously reported for the MMTV promoter [21], PR was found at the enhancers as early as 2 min after hormone addition. CHD8 was absent at 2 min but was found at the 5 min time point, remaining in the enhancers during the 15 and 30 min time points. Finally, RNAPII occupancy increased between 15 and 30 min at the FKPB5e and only at 30 min at NFE2L3e and IL6STe enhancers (Fig 8A). These data indicate that RNAPII is recruited to the enhancers after PR and CHD8 and suggest that RNAPII recruitment is a late event during the process of enhancer activation. Then, we investigated the role of CHD8 in RNAPII recruitment. Interestingly, hormone-stimulated RNAPII occupancy was strongly impaired in CHD8-depleted cells (Fig 8B). Next, eRNA synthesis at FKBP5e, NFE2L3e and IL6STe enhancers was evaluated by RT-qPCR both 45 min and 6 h upon R5020 addition (see Materials and Methods). Progestin increased between 2 and 7 fold production of eRNAs already at 45 min and expression was maintained after 6 h (Fig 8C). As a control, we verified that no qPCR signal was observed in the absence of reverse transcriptase. Consistently with the effect of CHD8 depletion in RNAPII occupancy, silencing of CHD8 significantly impaired eRNA synthesis from the three analyzed regions (Fig 8C). These results indicate that CHD8 is required for RNAPII recruitment and enhancer transcription, at least from a subset of PR enhancers.
We have recently reported a promoter ChIP-on-chip analysis demonstrating that, in cervix carcinoma C33 cells, CHD8 binds around 2000 active promoters also enriched in H3K4me2 and H3K4me3, and that it is required for expression of E2F-dependent G1/S transition genes [17]. In agreement with these results, our genome-wide ChIP-seq analysis reveals that most of CHD8 is bound to promoters also enriched in RNAPII and H3K4me3 in proliferating T47D-MTVL cells. While this paper was in revision another group has also shown that CHD8 binds thousand of active promoters in an iPSC-derived neuronal progenitor cell line [47]. All these data from different cell lines support that CHD8 is a promoter-associated factor. However, upon progestin stimulation CHD8 was rarely found at promoters; instead it was recruited to a number of PRbs mostly located in intergenic and intronic regions enriched in H3K4me1 and p300, indicating that these sites are progesterone-dependent enhancers. Interestingly, under normal proliferation conditions, CHD8 was found at the promoters of genes close to these enhancers (p < 0. 001). While this fact might suggest that CHD8 dynamically move from enhancers to close promoters, further experiments are required to demonstrate this association. Our data demonstrate that CHD8 binds both, promoters and enhancers upon specific stimulation such as progesterone. CHD8 has also been involved in AR-dependent [19] and ER-dependent [18] regulation. It is, therefore, possible that CHD8 also binds AR or ER enhancers in the presence of the appropriated hormonal stimulus. CHD7 is a paralogue of CHD8 and both proteins has been shown to interact [48]. ChIP-seq analysis of mice ES cells has demonstrated that CHD7 is mostly associated with a subset of active enhancers and promoters [49,50]. Comparison of CHD8 genomic distribution with that of CHD7 available from ENCODE showed that only 7. 1% and 5. 6% of the CHD8 peaks overlap with CHD7 containing regions in K562 or H1 stem cells, respectively. Most enhancers are tissue- and cell line-specific and therefore it is difficult to compare genomic positions between different cell lines. Nevertheless, these data suggest that although CHD7 and CHD8 may cooperate in some genomic locations, they also have specific independent roles. It has been reported that CTCF interacts with the carboxy-terminus of CHD8 and that CHD8 co-occupy several CTCF binding sites [11]. Our genome-wide study demonstrates that, in the absence of progesterone, 16. 5% of the CHD8 binding sites are enriched for CTCF and 4. 4% of CTCF sites are occupied by CHD8. While this percentage is largely higher than expected by chance, it is clear that CHD8 might only be involved in CTCF function in a small percentage of insulators. Since CTCF sites are often located at enhancer regions [51] and enhancer looping activity has been related to CTCF and cohesins [52], it is possible that the CHD8-CTCF co-occupancy is more related to the role of CHD8 in enhancers than to specific functions in insulation. In the presence of progestin the number of sites co-occupied by CTCF and CHD8 is even lower, suggesting that their association is not related to progesterone dependent transcription. Here we show that CHD8 is not recruited to four selected progesterone enhancers in the absence of PR, indicating that PR is essential for the recruiting. This is consistent with our time-course experiment where PR was found at the enhancers earlier than CHD8 upon hormone addition. However, we have been unable to detect direct PR-CHD8 interaction suggesting that CHD8 may be recruited through interaction with other co-regulators of PR enhancers. Despite the very significant overlapping between the pioneering factor FOXA1 and CHD8 binding sites, we show that FOXA1 silencing even increased CHD8 occupancy at the four analyzed enhancers, indicating that FOXA1 is not required for CHD8 recruitment. The chromatin remodelling BAF complex is recruited to PRbs [25] and is required for progesterone-dependent remodelling of the MMTV promoter [21,22]. Interestingly, we have observed that CHD8 interacts with both SWI/SNF complexes: BAF and PBAF. In addition, two high throughput proteomic analysis have identified co-immunoprecipitation of CHD8 and SWI/SNF subunits [53,54]. Since PR directly interacts with BAF complex it is possible that CHD8 is recruited to PRbs through interaction with the BAF complex. Consistently with this hypothesis, knocking down of BRG1 and BRM reduced CHD8 recruiting. Interestingly, it has been reported that the CHD8 paralogue, CHD7, interacts with PBAF, the other human SWI/SNF complex [55]. Whether other CHD8 paralogues, CHD6 and CHD9, also interact with SWI/SNF complexes is unknown. It is also unclear the extent to which CHD8 and SWI/SNF complexes cooperate and whether they can have common and independent targets. It is worth noting that both, SWI/SNF complexes and CHD8 are required for activation of E2F-dependent genes at the G1/S transition [17] [56]. On the other hand, we have reported that the chromodomains of CHD8 binds dimethylated and trimethylated H3K4 peptides [16]. H3K4me2 modification is typically associated with enhancers [57,58,59]. In addition, Vicent et al. reported that H3K4 methylation by the ASCOM (ASC-2 [activating signal cointegrator-2] complex) is required for the progesterone-dependent induction of the MMTV promoter [21]. Therefore, it is possible that CHD8 chromodomains interaction with methylated H3K4 also contributes to the recruitment or the stabilization of CHD8 at PR enhancers. We have shown that CHD8 depletion does not impair PR binding. Since PR directly interacts with BAF complex it is unlikely that CHD8 is required for BAF recruitment or activity. In fact, we show that BRG1 and BRM are required for normal CHD8 occupancy of PR enhancers suggesting that CHD8 acts downstream of the BAF complex. Another common step of enhancer activation is acetylation of H3K27 by the histone acetyltransferase p300 [29,36,37,38]. Ballare et al. , found that progestin-dependent PR biding sites were enriched in regions that contained p300 under un-stimulated conditions and that, in general, the level of p300 increased upon hormone treatment. We have observed that progestin stimulated H3K27 acetylation at the FKBP5e and IL6STe enhancers but not at the HSD11B2e and NFE2L3e enhancers. Depletion of CHD8 did not affect levels of H3K27Ac at any of the regions, suggesting that CHD8 was not involved in this step of enhancer activation. Numerous reports have evidenced that enhancer activation involves RNAPII recruiting and synthesis of eRNA, monodirectional or bidirectional transcripts of 0. 5 to 5 kb. Recent results demonstrate that eRNAs are involved in transcriptional activation of neighbouring genes, in enhancer-promoter looping and in directing chromatin-remodelling events at specific promoters [60,61,62,63,64]. The time-course experiment shown in Fig 8A indicates that RNAPII is recruited between 15 and 30 minutes after hormone stimulation, while PR and CHD8 reach the enhancers around 2 and 5 min after stimulation, respectively. We show that depletion of CHD8 strongly impairs recruiting of RNAPII and synthesis of eRNAs, demonstrating that CHD8 is required for these late events of progesterone enhancers activation. In Drosophila, the CHD8 orthologous Kismet is found at transcriptionally active genes in polytene chromosomes [65]. Kismet mutations reduce the level of phosphorylated elongating RNAPII but not the level of initiating RNAPII, suggesting that Kismet is involved in transcription elongation. Human CHD8 interacts with elongating RNAPII [16]. Furthermore, CHD8-depleted cells are hypersensitive to drugs that inhibit phosphorylation of serine 2 of the carboxy-terminal domain (CTD) of POLR2A, the largest subunit of RNAPII (DRB and flavopiridol), an early step of the transcription cycle [16]. These data suggest that, as Kismet, CHD8 may also be involved in elongation. Kaikkonen et al. , have recently reported that eRNA synthesis is sensitive to flavopiridol [58], suggesting that eRNA synthesis also requires CTD serine 2 phosphorylation. Therefore, it is possible that CHD8 is required for transcription elongation of eRNAs at PR enhancers. We also show that CHD8 is required to increase DNase I sensitivity at enhancers upon hormone treatment, suggesting that CHD8 may be involved in the hormone-dependent nucleosomal remodeling that occurs at a subset of PRbs [25]. It is well known that transcription increases DNase I sensitivity of gene bodies [66,67]. Therefore, it is also possible that CHD8 effect on DNase I sensitivity is caused by its role in enhancer transcription. Vicent et al. demonstrated that BAF and NURF chromatin remodelling machines are required for PR-dependent activation of the MMTV promoter and other PR enhancers [22,25]. Now we add a third actor, CHD8, to the list of remodelers required for progesterone-dependent activation. However, CHD8 seems to be recruited only to at subset of PRbs, including the MMTV promoter. Future experiments will be required to find what determines CHD8 recruitment to some PRbs and not to others. It is worth noting that it has been reported that CHD9 interacts in vitro with nuclear receptors such as PPARA (PPARα), NR1I3 (CAR), NR3C1, ESR1 (ERα) and RXRA [68,69]. So, it is tempting to speculate that other CHD8 paralogues may also regulate, redundantly or non-redundantly with CHD8, hormone-dependent enhancers activation.
T47D-MTVL human breast cancer cells carrying one stably integrated copy of the luciferase reporter gene under the control of the MMTV promoter [28] and T47D-YV cells (PR-negative clonal derivative cell line of T47D [32,70]) were routinely grown in RPMI 1640 medium supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin and 100 mg/ml streptomycin. Cells were grown exponentially (in 10% FBS) or subjected to serum-free conditions in RPMI medium without phenol red during 48 h. After serum starvation, cells were incubated with 10 nM R5020 or vehicle (ethanol; EtOH) for the indicated times. ChIP assays were performed as described [71] using anti-CHD8 (A301-224A) from Bethyl Laboratories or home-made rabbit anti-CHD8 [16], anti-RNAPII (N-20) (sc-899), anti-PR (H-190) (sc-7208), anti-BAF155 (R-18) (sc-9746) and anti-FOXA1 (H-120) (sc-22841) from Santa Cruz Biotechnology, and anti-H3K27Ac (ab4729) from Abcam. Chromatin was sonicated to an average fragment size of 400 to 500 bp using the Diagenode Bioruptor. Rabbit IgG (Sigma) was used as a control for non-specific interactions. Input was prepared with 10% of the chromatin material used for immunoprecipitation. Input material was diluted 1: 10 before PCR amplification. Quantification of immunoprecipitated DNA was performed by real-time PCR (qPCR) with the Applied Biosystems 7500 FAST real-time PCR system, using Applied Biosystems Power SYBR green master mix. Sample quantifications by qPCR were performed in triplicate. Sequences of all oligonucleotides are available upon request. Data are the average of at least three independent experiments. ChIP was performed as described above using anti-CHD8 (A301-224A, Bethyl Laboratories). ChIP-DNA was purified and subjected to deep sequencing using the Solexa Genome Analyzer (Illumina). The sequence reads were aligned to the human genome reference (assembly hg19). ChIP-seq peak calling, genomic annotation of peaks and comparison between ChIP-seq and ENCODE datasets were performed using ChIPseeqer (v. 2. 1) [72]. An empirical approach was followed to estimate the FDR, which involves using control data set as ChIP-seq data and the ChIP-seq data as the pseudo-control data and running peak detection. The FDR is defined as the ratio of the number of peaks detected in this pseudo-control analysis, to the number of peaks detected in the real ChIP-seq experiment. Motif analysis was performed using FIRE algorithm [73], included in the ChIPseeqer framework, MEME suite [74], Weeder PScan [75] and TRANSFAC database [76]. CHD8 ChIP-seq data are available from the GEO database (accession number GSE49134). RNAPII, H3K4me3 and PR ChIP-seq data in T47D were previously reported [25]. GEO accession number for FOXA1 and CTCF ChIP-seq data are GMS803409, GSM803348. All siRNAs were transfected using Oligofectamine (Invitrogen) according to the manufacturer’s instructions, with following siRNA sequences: for siCHD8,5′-GAGCAAGCUCAACACCAUC-3′; siFOXA1,5′-GAGAGAAAAAAUCAACAGC-3′; siCt, 5′-CGUACGCGGAAUACUUCGA-3′; siBRG1,5′-GCGACUCACUGACGGAGAA-3′; and siBRM, 5′-GAAAGGAGGUGCUAAGACA-3′. After transfection, medium was replaced by serum-free fresh medium without phenol red. After 48 h in serum-free conditions, cells were treated with 10 nM R5020 or vehicle (EtOH) for the indicated times. The down-regulation of CHD8, FOXA1, BRG1 and BRM was confirmed by RT-PCR and Western blotting, respectively. T47D-MTVL cells were grown in serum-free conditions, as explained previously, during 48 h and treated with 10 nM R5020 or vehicle (EtOH) during 6 h. Total RNA was isolated in triplicate from cells using RNeasy Mini Kit (Qiagen). Purity and quality of isolated RNA were assessed by RNA 6000 Nano assay on a 2100 Bioanalyzer (Agilent Technologies, Santa 6 Clara, CA). RNA (100 ng) was used for production of end-labelled biotinylated ssDNA. Labelled ssDNA was hybridized to the GeneChip human Gene 1. 0 ST array oligonucleotide microarray (Affymetrix, Santa Clara, CA) according to manufacturer’s recommendations. The arrays were scanned using the GeneChip Scanner 3000 7G (Affymetrix), and raw data were extracted from the scanned images and analyzed with the Affymetrix GeneChip Command Console Software (Affymetrix). The raw array data were pre-processed and normalized using the Robust Multichip Average (RMA) method [77]. Data were further processed using oneChannelGUI [78]. The log2 intensities for each probe were used for further analysis. Genes where considered as hormone induced when change of gene expression was >1. 5 (linear fold change) and p-value < 0. 01. Gene expression was considered as CHD8-dependent when [hormone-dependent change in siCHD8]/[hormone-dependent change in siCt] was higher than 1. 20 or lower than 0. 8. Microarray data are available from the GEO database (accession number GSE62257). DNase I assay was performed as previously described [40]. Briefly, 2 μg of crosslinked chromatin were treated with 0. 5,1, and 2 units of DNase I (Roche) for 3 min at 37°C. Control samples were incubated in the absence of DNaseI. Reactions were stopped by adding EDTA and the crosslinking was reversed by incubating the samples at 65°C. DNA was then isolated, quantified and used as template for qPCR reactions using specific primers. Total RNA was prepared by using the RNeasy Kit (Qiagen), as described in the manufacturer’s instructions; note that the step of DNase I digestion was included to avoid potential DNA contamination. cDNA was generated from 800 ng of total RNA (for progesterone-dependent mRNA induction analysis) using Superscript First Strand Synthesis System (Invitrogen). For progesterone-dependent eRNA induction analysis 2 μg of total RNA was used. In the case of FKBP5e and HSD11B2e eRNA determination, strand-specific oligonucleotides were used for RT, in order to avoid expression form close promoters. cDNA (2 μl) was used as a template for qPCR. Gene products were quantified by real-time PCR with the Applied Biosystems 7500 FAST real-time PCR system, using Applied Biosystems Power SYBR green master mix. Sequences of all oligonucleotides are available upon request. Values were normalized to the expression of the 28S housekeeping gene. Each experiment was performed at least in duplicate, and qPCR quantifications were performed in triplicate. Co-immunoprecipitations were performed as described in [17] using the anti-CHD8 antibody (A301-224A) from Bethyl Laboratories. Rabbit or mouse purified IgG (Sigma-Aldrich) were used as a control. 3% Input and precipitated proteins were separated by SDS/PAGE, and visualized by Western blotting with the indicated antibodies using ECL Plus (GE Healthcare), according to the manufacturer’s instructions. Antibodies used for western blotting were: anti-BRG1 (H88, sc-10768), anti-BAF155 (R-18, sc-9746), anti-BAF170 (E-6, sc-17838), anti-PR (H-190) (sc-7208) and anti-FOXA1 (H-120) (sc-22841), and anti-hSNF5 (C20, sc-16189) from Santa Cruz Biotechnology; anti-CHD8 (A301-224A) and anti-BAF180 (A301-590A) from Bethyl; anti-BAF250 (04–080) from Millipore; α-tubulin antibody (DM1A, T9026) from Sigma Aldrich and anti-BRM (ab15597) from Abcam.
A lot of research has been devoted during the last decades to understand the mechanisms that control gene promoters activity, however, much less is known about enhancers. Only recently, the use of genome-wide chromatin immunoprecipitation techniques has revealed the existence of more than 400,000 enhancers in the human genome. We are starting to understand the importance of these regulatory elements and how they are activated or repressed. In this work we discover that the chromatin remodeler CHD8 is recruited to Progesteron Receptor-dependent enhancers upon hormone treatment. CHD8 is required for late steps in the activation of these enhancers, including transcription of the enhancers and synthesis of eRNA (long noncoding RNAs derived form the enhancers).
The Chromatin Remodeler CHD8 Is Required for Activation of Progesterone Receptor-Dependent Enhancers