Many animals exploit several niches sequentially during their life cycles, a fitness referred to as ontogenetic niche shift (ONS). To successfully accomplish ONS, transition between development stages is often coupled with changes in one or more primitive, instinctive behaviors. Yet, the underlining molecular mechanisms remain elusive. We show here that Leptinotarsa decemlineata larvae finish their ONS at the wandering stage by leaving the plant and pupating in soil. At middle wandering phase, larvae also switch their phototactic behavior, from photophilic at foraging period to photophobic. We find that enhancement of juvenile hormone (JH) signal delays the phototactic switch, and vise verse. Moreover, RNA interference (RNAi) -aided knockdown of LdPTTH (prothoracicotropic hormone gene) or LdTorso (PTTH receptor gene) impairs avoidance response to light, a phenotype nonrescuable by 20-hydroxyecdysone. Consequently, the RNAi beetles pupate at the soil surface or in shallow layer of soil, with most of them failing to construct pupation chambers. Furthermore, a combination of depletion of LdPTTH/LdTorso and disturbance of JH signal causes no additive effects on light avoidance response and pupation site selection. Finally, we establish that TrpA1 (transient receptor potential (TRP) cation channel) is necessary for light avoidance behavior, acting downstream of PTTH. We conclude that JH/PTTH cascade concomitantly regulates metamorphosis and the phototaxis switch, to drive ONS of the wandering beetles from plant into soil to start the immobile pupal stage.
Movements to stage-dependent resources, i. e. , ontogenetic niche shifts (hereafter ONS), occur in nearly 80% of animal taxa. The shifts enable animals to exploit several niches sequentially during their life cycles to meet stage-dependent nutritional requirements, to overcome stage-specific physiological limitations, and to reduce intraspecific competition between juveniles and adults. ONS is thus widely accepted as an evolutionary adaptation [1–4]. To successfully finish ONS, transition between development stages is often accompanied with changes in one or more primitive, instinctive behaviors, allowing inexperienced novices to obtain novel abilities to detect new environmental cues [5]. To date, however, the underlining mechanisms driving these behavior switches are still largely unexplored. Insects are a suitable animal group to explore the molecular modes of these instinctive behavioral switches. Throughout developmental excursion, most insects (Holometabolans) undergo four discrete periods (complete metamorphosis), characterized by the presence of a pupal stage between a feeding larva and a reproducing adult [6,7]. Sessile pupae are vulnerable to potentially harmful factors such as desiccation, predation, parasitism and pathogen infection. These latent mortal dangers drive a lot of Holometabolans shifting into less risky habitats for pupation [8–12]. For example, pupation in soil and other relatively inaccessible sites for predators and parasitoids (concealed placement) has been documented in almost all Holometabolan orders [8,12–15]. Insect final instar larval stage is divided into two sub-stages, the foraging and the wandering phases. While foraging final instar larvae display generally similar behaviors like previous instar animals, a wandering larva typically undergoes an ONS by leaving the food source and moving to a proper pupation site [16]. Obviously, positive phototaxis directs most foraging insect herbivores to reach plant top for tender plant tissues such as shoots, young leaves, buds and flowers that are more nutritious and less defended [17], whereas negative phototaxis facilitates the wandering larvae to reach pupation refuge in the dark, such as soil [5]. Accordingly, it can be reasonably hypothesized that the change from foraging to wandering stages should be coupled with a switch for phototactic behavior from photophilic to photophobic in most herbivorous Holometabolans. For a soil-pupated insect species, a wandering larva usually shows a sequence of three primary behavioral components before pupation: a) leaving the food, crawling to the ground and searching for a suitable location, b) mining into soil, and c) building a pupation chamber in soil [18–20]. Ecdysteroids (the major active component is 20-hydroxyecdysone, 20E), the products in a pair of prothoracic glands (PGs), activate the wandering behavior [18,21–23]. Up to now, however, the molecular mechanism elicits the phototaxis switch in insect herbivores remains elusive. In Drosophila melanogaster, larval phototaxis and behavioral responses have been described [5,24–27]. Unfortunately, photophobic is age-independent in the larvae [5,24]. At the foraging stage, a Drosophila larva feeds inside rotting fruits; it is strongly repelled by light and seeks for dark or less light-exposed surroundings [5,25–27]. Two pairs of neurons called NP394 (each pair in a hemisphere of central brain) are required to maintain light avoidance in the foraging phase. Modulating activity of NP394 neurons affects larval light preference [28]. The NP394 neurons turn out to be prothoracicotropic hormone (PTTH) -expressing cells [5]. These two pairs of PTTH-producing neurons release PTTH to concomitantly promote steroidogenesis and light avoidance during wandering stage of the final instar larvae [5]. On one hand, PTTH, through its receptor Torso, activates a canonical mitogen activated protein kinase (MAPK) pathway to trigger ecdysteroidogenesis by PGs to regulate metamorphosis [29–31]. On the other hand, PTTH/Torso complex acts on two light sensors, the Bolwig’s organ (a group of 12 photoreceptors in the larval eye) and the peripheral class IV dendritic arborization neurons, to reinforce light avoidance [5]. The young larvae of the Colorado potato beetle Leptinotarsa decemlineata, a notorious insect defoliator of potatoes, reveal a tendency to rest and feed on the upper surfaces of leaves during foraging stage [32]. At the late stage of the final (fourth) instar, conversely, the wandering larvae bury themselves into soil, where, after several days, they metamorphose into pupae [33,34]. Moreover, by RNA interference (RNAi), we have identified major components of the hormonal network that regulates larval metamorphosis in L. decemlineata [33–41]. This offers a great opportunity to explore the molecular mode driving the phototaxis switch in an insect herbivore. The first aim of the current study was to determine whether Leptinotarsa larvae changed their light preference from photophilic to photophobic during wandering stage. We then uncovered that juvenile hormone (JH) /PTTH cascade concomitantly regulated metamorphosis and the phototaxis switch. Finally, we provided clear evidence that PTTH-induced light avoidance drove ONS from plant to pupation refuge in an insect herbivore.
We first observed phototaxis of Leptinotarsa beetles on the natural potato field. The females unselectively deposited their egg masses on upper and lower leaf surfaces (for a total of 100 egg masses 48 and 52 respectively on upper and lower surfaces, P>0. 05 for χ2 test) at the inner part of the potato canopy (S1A and S1B Fig). Aggregated hatchlings consumed foliage near the egg mass from which they hatched (S1C Fig). All the second-, third- and foraging fourth-instar larvae were found to feed and rest on the upper surfaces of the potato leaves (S1D–S1F Fig). These larvae molted under sunny lighting condition (S1F Fig). During the wandering larval stage from approximately 4. 1 to 7. 0 days after ecdysis to the fourth instar (S1G–S1I Fig), the animals left the potatoes and crawled to the ground, walked along the ground (4. 5–5. 5 days post ecdysis), dug into soil, constructed their pupation chambers and pupated therein (5. 6–7. 0 days) (S1G–S1I Fig). When given a choice in the laboratory, second-instar, third-instar and foraging fourth-instar larvae prefer light-exposed over shaded areas (Fig 1). For wandering Leptinotarsa larvae, however, light avoidance index significantly increased at 4–5 days post ecdysis, and reached nearly 1. 0 at 5. 5 days (Fig 1). These basic findings demonstrate that an obvious phototactic switch occurs at the middle wandering period. In the present paper, we intended to knock down target gene to study molecular modes underlining phototaxis switch using dietary dsRNA treatments. Although our previous results revealed that ingestion of dsRNA can silence target genes in various tissues including neurons, PGs and the corpora allata that producing JH [33–41], we have not compared the RNAi efficacy of dsRNA ingestion with that of dsRNA injection. Here we determined time-effect and concentration-effect curves of two genes, a nutrient amino acid transporter gene LdNAT1 that is mainly expressed in gut [42] and a transient receptor potential cation channel gene LdTrp that is highly transcribed in eyes [43]. Our results showed that injected and fed dsRNAs could reduce approximately 90% of LdNAT1 and LdTrp mRNAs 24 and 36 hours after treatment (S2A and S2B Fig). Moreover, RNAi efficacies are dose-dependent, no matter the dsRNAs are introduced by ingestion (S2C and S2D Fig) or injection (S2E and S2F Fig). In L. decemlineata, transition from foraging to wandering phases is associated with drastic level fluctuations of three larval hormones: 20E, insulin-like peptide (ILP) and JH [33,34,36,40,44]. Are these hormone signaling cascades involved in the regulation of phototaxis switch? In the final larval instar of L. decemlineata, a small 20E rise appears at 4 days after ecdysis [42]. Here, we found that dietary supplement with 20E to generate a premature 20E peak at the foraging stage (Fig 2A), or knockdown of an ecdysteroidogenesis gene (Shade, SHD) [38] to remove this 20E rise (Fig 2B and S3A Fig), did not affect the phototaxis switch, neither did silencing of a 20E receptor gene EcR (Fig 2C and S3B Fig) or a 20E cascade gene HR3 (Fig 2D and S3C Fig) to eliminate 20E signal. Conversely, wandering behavior occurred at 4. 5–4. 7 days after ecdysis (S1 Table), ingestion of 20E accelerated the onset of wandering behavior, whereas interruption of 20E signal retarded the onset (S1 Table). This piece of clear evidence demonstrates that different signal cascades respectively regulate the light preference and the onset of wandering behavior. Cessation of feeding decreased the contents of several nutrients in the larval hemolymph and thereby reduced ILP level during wandering stage [36]. Here we found that premature insulin deficiency brought about by knockdown of ILP2 (S3D Fig) had little effect on light avoidance (Fig 2E), but delayed the onset of wandering (S1 Table). At the wandering stage, JH titer obviously decreased [41], and the expression of JH degradation genes were activated [39]. Here we found that ingestion of JH (Fig 2F), or knockdown of an allatostatin gene (allatostatin C, AS-C) [41] or either of two JH degradation genes (JH epoxide hydrolase, JHEH1; JH diol kinase, JHDK) [35,39] to delay JH decrease significantly reduced light avoidance (Fig 2G and S3E–S3G Fig) and postponed the occurrence of wandering (S1 Table); whereas knockdown of a JH biosynthesis gene (JH acid methyl transferase, JHAMT) [34] and a JH receptor gene (methoprene-tolerant, Met) (to decrease the accumulated proteins) to prematurely reduce JH signal enhanced light avoidance (Fig 2H and S3H and S3I Fig) and accelerated the onset of wandering (S1 Table). It is clear that JH signal concomitantly inhibits the premature switch of light preference and the early onset of wandering. Providing elimination of JH is a prerequisite for successful PTTH release and signal transduction [45], we determined the expression levels of two PTTH signaling genes (LdPTTH and LdTorso) in the larvae whose JH signal had been disturbed. As expected, the mRNA levels of the two genes were reduced in the larval specimens whose JH signal had been enhanced (S4A–S4D and S5A–S5D Figs), and were increased in the larval samples in which JH signal had been repressed (S4E, S4F, S5E and S5F Figs). It is suggested that PTTH promotes larval light avoidance in L. decemlineata. We next knocked down PTTH gene (Fig 3A), and verified the knockdown by determination of the mRNA level of an ecdysteroidogenesis gene (LdPHM) and 20E titer in the resultant larval specimens (Fig 3B). The pupation rate was decreased and the development time was lengthened in LdPTTH RNAi (and PTTH depleted) larvae; dietary supplement with 20E can rescue the two phenotypes (Fig 3C and S1 Table). Moreover, silencing of PTTH reduced avoidance response to light (Fig 3D), rate of larvae that had buried in soil per day (RLB) (Fig 3E) and accumulated RLB (Fig 3F). Approximately 20% of wandering larvae excavated only a slight depression at the soil surface and pupated (Fig 3G and 3H. Please note, photo in Fig 3G was collected from a separate experiment). Furthermore, the remaining around 80% LdPTTH RNAi larvae pupated at shallow layer of soil (Fig 3I); most of them did not construct pupation chambers (Fig 3J). Dietary supplement with 20E could not alleviate the reduced light avoidance response and the negative influences on pupation in LdPTTH depleted larvae (Fig 3D–3J). We repeated the bioassay by knockdown of another PTTH pathway gene, LdTorso [46], and obtained similar results (S5 Fig and S1 Table. Please note, photo in S5M Fig was collected from a separate experiment). We then examined the relative mRNA levels of a JH biosynthesis gene LdJHAMT and two JH signaling pathway genes (LdMet and LdKr-h1), and found that the levels of these genes varied little in LdPTTH or LdTorso RNAi larvae when measured 1 and 2 days post ecdysis to fourth-instar larvae (S4G and S5Q–S5S Figs). We further investigated the combination effects of PTTH and JH signaling pathways on phototaxis switch by depletion of LdPTTH and disturbance of JH signal (Fig 4 and S6 Fig, S1 Table). We found no additive effect on avoidance response to light (Fig 4A–4D), accumulated rate of larvae in soil (Fig 4E–4H) and rate of pupae on the soil (Fig 4I–4L) by knockdown of LdPTTH and enhancement of JH signal (an addition of JH, or RNAi of LdAS-C), or silencing of LdPTTH and reduction of JH titer (RNAi of LdJHAMT) or JH signal (silencing of LdMet). Knockdown of LdTorso and disturbance of JH pathway mimicked the combination effects on the larval phototaxis switch (S7 Fig). These results show that JH and PTTH act on the same pathway to promote light avoidance. Our previous results showed that four PTTH/Torso cascade genes were highly or moderately expressed in the brain [46], suggesting that PTTH may act on neuronal cells to control light avoidance. Consistent with the suggestion, we found that the relative mRNA levels of LdTrpA1 that encodes transient receptor potential (TRP) cation channel in Drosophila [47] were significantly decreased in LdPTTH and LdTorso depleted larvae. In contrast, the levels of LdRh5 (the opsin gene involved in light avoidance behavior in Drosophila) [28] and LdGr28b (a gustatory receptor family gene that plays an opsin-like role in class IV da neurons in Drosophila) [48] were not affected (S8 Fig). It appears that PTTH/Torso exerts its action downstream of the photoreceptors, and upstream of TrpA1 channel activation. Accordingly, we observed depletion of LdTrpA1 reduced light avoidance response, and the accumulated number of larvae in soil, and increased the rate of pupae on the soil. Moreover, knockdown of both LdTrpA1 and LdPTTH, or LdTrpA1 and LdTorso showed no additive effects (Fig 5 and S9 Fig) (we dietarily supplemented 20E in all treatment to relieve the effect of knockdown on pupation and development time). Conversely, knockdown of LdTrp did not affect light avoidance response (S10 Fig). This provides strong evidence that PTTH/Torso signaling cascade activates a step in photo transduction between the photoreceptor molecule and the TRP channel.
PTTH is synthesized in two pairs of dorsolateral neurosecretory cells in the brain and transported to the corpora allata (CA), an endocrine organ that produces JH, by axons running through the contralateral hemisphere of the brain. PTTH is secreted into the hemolymph from arborized axon endings in the CA. The release of PTTH is negatively controlled by JH during the early stages of the last larval instar [29–31]. Similarly, our recent results revealed that JH signal plays an inhibitive role on PTTH production and release in Leptinotarsa larvae [45]. In this survey, we provide another three compelling pieces of evidence to support the conclusion. Firstly, we found reduced transcription levels of the two PTTH signaling genes were correlated with enhanced JH signal, and vise verse (S4 and S5 Figs). Secondly, JH signal concomitantly inhibited the premature switch of light preference and the early onset of wandering (Fig 2, S2 Table), two behaviors can be elicited by PTTH signaling [5,18,21]. Lastly, no additive effects on light avoidance response and pupation were observed by simultaneous inhibition of PTTH pathway and disturbance of JH signal (Fig 4). Insects such as L. decemlineata, Manduca sexta, D. melanogaster and Trichoplusia ni, must reach critical weight before larval-pupal transition [45,49–51]. When premature metamorphosis occurs below this weight, individuals tend to burden disproportionately high costs [49–52]. Therefore, it can be reasonably proposed that the presence of JH prevents premature PTTH release, and allows insect to obtain species-specific body size, before the onset of wandering and the switch of light preference. In L. decemlineata, the final-instar larvae obtained their maximum fresh weights at approximately 84 hours after ecdysis [45]. At this time the JH should be completely removed, just as that in M. sexta [49]. The elimination of JH allows the brain to release PTTH. Therefore, the release of PTTH occurs at approximately 84 hours after ecdysis, i. e. , around 12 hours before the small 20E rise at day 4 [44]. In this study, we found the wandering behavior occurred on 4. 5–4. 7 days (S1 Table). The latency between appearance of 20E and the onset of wandering is approximately 12–15 hours in Leptinotarsa, similar to the dormancy time in Manduca [22,23]. Accordingly, it can be estimated that the latency time from the release of PTTH to the occurrence of wandering is around a day. Moreover, we found here that the phototaxis switch had finished on day 5. 5–6. 0 post ecdysis (Fig 1). The latency time from the release of PTTH to the phototaxis switch is around two day. The same dormancy time was noted from LdPTTH or LdTorso RNAi larvae, the enzymatic removal of dsRNA caused the restoration of the mRNA levels of LdPTTH or LdTorso 5–6 days after initiation of dsRNA ingestion (S2 Fig) and the re-activation of transcription of PTTH and Torso finally occurred hereafter; high level of mRNAs were tested at 7 days after ecdysis (Fig 3 and S5 Fig). This means that functional PTTH/Torso proteins are produced before 7 days after ecdysis. Therefore, the PTTH and Torso RNAi larvae are drove to burrow into soil around 2 days after functional PTTH/Torso proteins are produced, with peaked RLB values at about 8 days (Fig 3 and S5 Fig). In contrast, PTTH in Drosophila was secreted into the hemolymph and reached two light sensors, the Bolwig’s organ and the peripheral class IV dendritic arborization neurons involved in light avoidance. Inactivation of PTTH-expressing neurons affected light avoidance with 8 to 10 hours delay [5]. In this study, we transferred L. decemlineata final-instar larvae to soil at 4 days post ecdysis (the small 20E rise occurs within this day [44]). During the period from the appearance of wandering behavior to the occurrence of phototaxis switch, the Leptinotarsa wandering larvae kept crawling at the soil surface; they did not burrow into soil (Fig 3 and S5 Fig) until the phototaxis switch had finished (Fig 1). Therefore, PTTH-induced phototaxis switch drives Leptinotarsa larvae to burrow into soil. From an ecological point of view, it seems an important evolutionary fitness for wandering Leptinotarsa larvae to crawl at the soil surface for an average of 24 hours before the switch of phototaxis. During the 24 hours’ crawling period, the larvae walk a long distance away from damaged plants to complete ONS before pupation. Since herbivore-induced plant volatiles emitted by damaged plants [53,54] attract natural enemies, maximizing distance from damaged plants prior to pupating increases the likelihood of survival. Therefore, respective regulation of the onset of wandering and the switch of phototaxis by two distinct PTTH signal branches may be a molecular evolutionary approach for insect herbivores to shift into less risky habitats for pupation. Conversely, a portion of Drosophila larvae begin wandering at 108 hours after egg laying (AEL), and almost all the larvae enter wandering stage at 120 hours AEL. Comparably, some larvae start to pupariate at 108 hours AEL and almost all the larvae finish pupariation at 120 hours AEL [5]. This finding demonstrates that Drosophila larvae immediately form puparium even at the very beginning of the wandering period when they find an appropriate pupariation site. Consistent with the finding, the interval between PTTH release and the reinforce of light avoidance was 8 to 10 hours, while the latency time of 20E release and the occurrence of wandering is approximately 4–6 hours [55]. Considering the latency between PTTH release and ecdysone release in PGs, it is obvious that two PTTH-induced signal branches simultaneously trigger wandering behavior and reinforce light avoidance in Drosophila larvae. Therefore, other cues rather than phototaxis switch decide where wandering Drosophila larvae pupariate. Actually, it is believed that hydrotaxis (seeking for not moisture environment) drives wandering Drosophila larvae to leave the food source and find a suitable pupation site [56]. It is well known that ecdysteroidogenesis in PGs are redundantly regulated by several tropic signals, such as PTTH, ILP, target of rapamycin, transforming growth factor-β/Activin and nitric oxide signals [57]. In agreement with this accepted notion, our results revealed that the wandering behavior was still activated in PTTH and Torso RNAi larvae, with a retardation of about a day (S1 Table). However, the reduced PTTH signal cannot trigger the light avoidance behavior to drive LdPTTH and LdTorso RNAi larvae to mine into soil; the beetles keep crawling for a longer period of time compared with controls (Fig 3 and S5 Fig). Due to extended crawling period, PTTH and Torso RNAi beetles have less time to tunnel into soil and build their pupation chambers before the big 20E peak that elicits pupation [42]. As a result, we found in this survey that PTTH or Torso RNAi larvae pupated at the soil surface or at shallow layer of soil, with unfinished pupation chambers. Similarly, Manduca larvae can construct their pupation cells when they are placed in the observation chambers during the first 20 hours of wandering. At 30 hours, larvae begin to lose their ability to complete the pupation cell. By 35–40 hours, the larvae dig only a slight depression at the soil surface before pupation [58]. Accordingly, we argue that relative constant time interval between the onset of wandering and the switch of phototaxis is crucial for an insect herbivore to accomplish ONS during the final instar to correct pupation site, a trait potentially beneficial for ecological selection. If the interval is too short, the wandering larvae have no time to choose less risky habitats for pupation. If it is too long, the wandering insects have no time to tunnel into soil and construct chambers before pupation. JH/PTTH signaling is thus at the core of a hormonal network that coordinates developmental progression and appropriate phototactic behavior to maximize insect fitness. We propose a model summarizing these findings (Fig 5E). Although the conclusions are drawn using feeding-based RNAi knockdown and pharmacological application of hormones in whole beetle, many important genes involved in this survey such as PTTH, Torso, most ecdysteroidogenesis genes, AS-C and JHAMT, are only expressed in specific neurons or endocrine organs. Knockdown of these genes in whole animal only affects these neurons and endocrine organs. As a result, our findings are comparable to those from Drosophila [5,28], even though many manipulations are done at cellular levels in the fly. It seems that PTTH-droved (reinforced in Drosophila) light avoidance is a conserved trait to facilitate the wandering larvae to find suitable pupation sites. As a result, RNAi of the related genes makes the juveniles to be exposed to latent mortal dangers [8–12], and is a potential dsRNA-based method to control the agricultural pest.
The L. decemlineata beetles were kept in an insectary according to a previously described method [59], with potato foliage at the vegetative growth or young tuber stages in order to assure sufficient nutrition. At this feeding protocol, the larvae progressed through the first, second, third, and fourth instars at an approximate period of 2,2, 2 and 4 days, respectively. In a 15-hectare potato field located at Urumqi city (43. 82 N, 87. 61 E) in the Xinjiang Uygur autonomous region of China, 100 egg masses were selected randomly and marked along a diagonal line in June 15,2017. The development was observed and recorded at an interval of 4 hours (at night, the larvae were observed under red light) until all the larvae left the plants. The same method as previously reported [5,27,28] was used to test light avoidance of the larvae, with slight modifications. To synchronize the developing stage, newly-ecdysed larvae (the second through fourth instar larvae) were collected at an interval of 4 hours, and determined light avoidance at specific developing stage and different treatment, according to the experimental schedule (see Fig legend for detail). Five larvae were subjected to 20 and 30-min phototaxis assay in a Petri dish (9 cm diameter and 1. 5 cm height, half of which is covered with black paper) which was illuminated from above using a white LED light at 500 lux (a continuous range of radiated wavelengths from 400 to 700 nm, peak at 470 nm). The larvae were placed on the center spot along the junction line between light and dark, and the larvae on which half was recorded after 20 and 30 min at a constant temperature of 25°C. Ten larvae were set as a repeat, the assay repeated six times, a total of 60 larvae were determined for each instar. A steady state was reached after 20 min and we did not find any difference in the results after 20 or 30 min. The following formula for Light Avoidance Index was used: LightAvoidanceIndex= (numberoflarvaeindark–numberoflarvaeinlight) / (totalnumberoflarvae). For bacterially expressed dsRNA, specific primers used to clone dsRNAs were listed in S2 Table. These dsRNAs were from the fragments of genes lined in Data Accessibility. They were individually expressed using Escherichia coli HT115 (DE3) competent cells lacking RNase III following the established method [60]. Individual colonies were inoculated, and grown until cultures reached an OD600 value of 1. 0. The colonies were then induced to express dsRNA by addition of isopropyl β-D-1-thiogalactopyranoside to a final concentration of 0. 1 mM. The expressed dsRNA was extracted and confirmed by electrophoresis on 1% agarose gel. Bacteria cells were centrifuged at 5000 ×g for 10 min, and resuspended in an equal original culture volume of 0. 05 M phosphate buffered saline (PBS, pH 7. 4). The bacterial solutions (at a dsRNA concentration of about 0. 5 μg/ml) were used for experiment. For lab-synthesized dsRNA, LdNAT1 and LdTrp fragments were amplified by PCR using specific primers conjugated with the T7 RNA polymerase promoter (primers listed in S2 Table). The dsRNA originated from each of the above-mentioned sequences was synthesized using the MEGAscript T7 High Yield Transcription Kit (Ambion, Austin, USA) according to the manufacturer' s instructions. Subsequently, the synthesized dsRNA was determined by agarose gel electrophoresis and the Nanodrop 1000 spectrophotometer and kept at -70 °C until use. The same method as previously reported [60] was used to individually introduce bacterially expressed dsRNAs listed in S1 Table into larvae. Potato leaves were immersed with a bacterial suspension containing a dsRNA for 5 s, removed, and dried for 2 h under airflow on filter paper. The PBS- and dsegfp (enhanced green fluorescent protein) -dipped leaves were used as controls. Five treated leaves were then placed in Petri dishes (9 cm diameter and 1. 5 cm height). For knockdown of LdSHD, LdEcR and LdHR3, the newly-ecdysed fourth-instar larvae were used. For other dsRNA feeding bioassays, the newly-ecdysed third-instar larvae were used. The larvae were starved for at least 4 h prior to the experiment. Then, ten larvae were transferred to each dish as a repeat. The larvae were allowed to feed treated foliage for 3 days (replaced with freshly treated ones each day), and were transferred to untreated foliage if necessary. For lab-synthesized dsRNA, it was injected ventrally between two segments of the abdomen of the newly-ecdysed forth-instar larvae with 6 μl of dsRNA. For testing time-effect curve, 50 ng of dsNAT1 or dsTrp was injected into hemolymph. For measuring concentration-effect curve, each dsRNA was injected at a dose of 100. 0,25. 0,6. 3 and 1. 3 ng. 20-Hydroxyecdysone (20E) (Sigma-Aldrich, USA) and juvenile hormone (JH) (Sigma-Aldrich, USA) were respectively dissolved in distilled water with added surfactant (Tween 20,1 g/L) to obtain two solutions at the concentration of 10 ng/mL. Potato leaves were dipped with 20E or JH solution. 20E supplemented leaves were provided at day 3 fourth-instar larvae, whereas JH supplemented leaves were offered at newly-ecdysed fourth-instar stage. The larvae were allowed to feed the foliage for a day. Total RNA was extracted from treated and control larvae. Each sample contained 5–10 individuals and repeated three times. The RNA was extracted using SV Total RNA Isolation System Kit (Promega). Purified RNA was subjected to DNase I to remove any residual DNA according to the manufacturer’s instructions. Quantitative mRNA measurements were performed by qRT-PCR in technical triplicate, using 4 internal control genes (LdRP4, LdRP18, LdARF1 and LdARF4, see S1 Table) according to our published results [59]. An RT negative control (without reverse transcriptase) and a non-template negative control were included for each primer set to confirm the absence of genomic DNA and to check for primer-dimer or contamination in the reactions, respectively. According to a previously described method [61], the generation of specific PCR products was confirmed by gel electrophoresis. The primer pair for each gene was tested with a 10-fold logarithmic dilution of a cDNA mixture to generate a linear standard curve (crossing point [CP] plotted vs. log of template concentration), which was used to calculate the primer pair efficiency. All primer pairs amplified a single PCR product with the expected sizes, showed a slope less than -3. 0, and exhibited efficiency values ranging from 2. 0 to 2. 1. Data were analyzed by the 2-ΔΔCT method, using the geometric mean of the four internal control genes for normalization. 20E was extracted according to a ultrasonic-assisted extraction method [40], and its titer (ng per g body weight) was analyzed by an LC tandem mass spectrometry-mass spectrometry (LC-MS/MS) system using a protocol the same as described [62]. Hemolymph was collected and JH was extracted following the methods described previously [60]. LC-MS was used to quantify JH titers (ng per ml hemolymph) [63]. The data were given as means ± SE, and were analyzed by analyses of variance (ANOVAs) followed by the Tukey-Kramer test, using SPSS for Windows (SPSS, Chicago, IL, USA), or t test. Light preference index, light avoidance index (LAI), rate of larvae that had buried in soil per day (RLB), accumulated RBP (ARLB), rate of pupae on soil (RPS), and rate of pupae that had constructed pupation chambers (RPC) were subjected to arc-sine transformation before ANOVAs.
Many animals occupy distinct niches and utilize diverse resources at different development stages in order to meet stage-dependent requirements and overcome stage-specific limitations. This fitness is referred to as ontogenetic niche shift (ONS). During the preparation for ONS, animals often change one or more primitive, instinctive behaviors. Holometabolous insects, with four discrete developmental periods usually in different niches, are a suitable animal group to explore the molecular modes of these behavioral switches. Here we find that Leptinotarsa decemlineata larvae, an insect defoliator of potatoes, switch their phototactic behavior, from photophilic at feeding period to photophobic during the larval-pupal transition (wandering stage). This phototactic switch facilitates the wandering larvae to accomplish the ONS from potato plants to their pupation sites below ground. We show that JH/PTTH cascade controls the phototaxis switch, through a step in photo transduction between the photoreceptor molecule and the transient receptor potential cation channel.
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Hormonal signaling cascades required for phototaxis switch in wandering Leptinotarsa decemlineata larvae