Enterotoxigenic Escherichia coli (ETEC), defined by their elaboration of heat-labile (LT) and/or heat-stable (ST) enterotoxins, are a common cause of diarrheal illness in developing countries. Efficient delivery of these toxins requires ETEC to engage target host enterocytes. This engagement is accomplished using a variety of pathovar-specific and conserved E. coli adhesin molecules as well as plasmid encoded colonization factors. Some of these adhesins undergo significant transcriptional modulation as ETEC encounter intestinal epithelia, perhaps suggesting that they cooperatively facilitate interaction with the host. Among genes significantly upregulated on cell contact are those encoding type 1 pili. We therefore investigated the role played by these pili in facilitating ETEC adhesion, and toxin delivery to model intestinal epithelia. We demonstrate that type 1 pili, encoded in the E. coli core genome, play an essential role in ETEC virulence, acting in concert with plasmid-encoded pathovar specific colonization factor (CF) fimbriae to promote optimal bacterial adhesion to cultured intestinal epithelium (CIE) and to epithelial monolayers differentiated from human small intestinal stem cells. Type 1 pili are tipped with the FimH adhesin which recognizes mannose with stereochemical specificity. Thus, enhanced production of highly mannosylated proteins on intestinal epithelia promoted FimH-mediated ETEC adhesion, while conversely, interruption of FimH lectin-epithelial interactions with soluble mannose, anti-FimH antibodies or mutagenesis of fimH effectively blocked ETEC adhesion. Moreover, fimH mutants were significantly impaired in delivery of both heat-stable and heat-labile toxins to the target epithelial cells in vitro, and these mutants were substantially less virulent in rabbit ileal loop assays, a classical model of ETEC pathogenesis. Collectively, our data suggest that these highly conserved pili play an essential role in virulence of these diverse pathogens.
Among young children under five years of age in developing countries, diarrhea is a leading cause of morbidity and mortality. Enterotoxigenic E. coli (ETEC) is one of the most common causes of moderate to severe diarrheal illness and deaths due to diarrhea in young children and incidentally is also the leading bacterial cause of diarrhea [1]. These bacteria are also a leading cause of hospitalization due to severe diarrhea in adults in developing countries [2] and are perennially the predominant cause of diarrheal illness among travelers to the endemic regions [3,4]. Additionally, ETEC infections contribute substantially to the burden of diarrheal illness associated with sequelae of malnutrition [5,6], stunted growth [7] and impaired cognitive development [8]. The effects of ETEC infections also appear to be more critical in malnourished children [5]. Thus these pathogens contribute to a complex pattern of poverty, repeated enteric infections, environmental enteropathy [9], and developmental impairment. ETEC are defined by the production of heat-labile (LT) and/or heat-stable (ST) enterotoxins [10], and virulence requires successful delivery of these toxins to cognate receptors on target intestinal epithelial cells. LT binds to cell surface GM1 gangliosides, and following cellular entry this toxin activates production of host cAMP; while ST peptides bind guanylate cyclase C, stimulating production of cGMP [11]. Resulting increases in intracellular concentrations of these cyclic nucleotides modulate ion channels on the surface of intestinal cells leading to net losses of sodium chloride and water into the intestinal lumen and ensuing acute watery diarrhea [11,12]. In the classical paradigm of ETEC pathogenesis, these bacteria utilize pathovar-specific fimbrial or non-fimbrial adhesins, known as colonization factors or CFs [13] which allow them to adhere and colonize the small intestine where toxin delivery occurs. However, emerging evidence would suggest that this paradigm is perhaps overly simplistic, and that there are several potential adhesins which effectively act in concert to promote ETEC engagement of the host [14]. E. coli encode a multitude of pili assembled by the chaperone/usher pathway, termed CUP pili, which are important in virulence. CUP pili are tipped with specialized adhesins that recognize specific receptors with stereochemical specificity. CUP adhesins can determine both tissue tropism and the course of disease. For example, type 1 pili are encoded by the fim operon, and chomosomally encoded as part of the core E. coli genome [15,16]. Type 1 pili are composite fibers comprised of a pilus rod, made up of FimA subunits arranged in a right handed helical cylinder [17]. The pilus rod is joined to a fibrillum structure tipped with the FimH adhesin that binds mannose with stereochemical specificity [16,18–20]. FimH is critical for virulence in extraintestinal E. coli, as it has been well-established that FimH mediated adhesion enables uropathogenic E. coli (UPEC) colonization and invasion into bladder epithelial cells [21–23], as well as the formation of intracellular bacterial communities [24]. ETEC also encode pathovar-specific CUP pili (fimbriae) like CFA/I [25] that are encoded on virulence plasmids [26]. More than four decades ago, these ETEC-specific colonization factors were shown to contribute to development of diarrheal illness in humans [27], and consequently they have been the subject of intensive investigation and a major focus of ETEC vaccine development. Conversely, although early studies described possible type 1 pili expression by ETEC [28,29], relatively little is known about the contribution of these highly conserved structures to virulence. Our more recent observation that the expression of the fim operon is enhanced by pathogen-host cell contact [14], prompted a thorough investigation of the potential role of type 1 pili in ETEC pathogenesis reported here.
Although a number of earlier studies of ETEC suggested that these pathogens make type 1 pili (also previously referred to as type 1 somatic pili or type 1 fimbriae) [29,30], to date there has been no systematic examination of their involvement in ETEC virulence. We therefore first performed studies to confirm the production of type 1 pili by the prototypical ETEC H10407 strain. Type 1 pili tipped with the FimH adhesin were identified on the surface of strain H10407 by transmission electron microscopy after immunogold labeling using anti-FimH antibodies (Fig 1A). Similarly, using flow cytometry we verified production of type 1 pili in H10407, but not in the corresponding fimH mutant strain (Fig 1B). It has previously been shown that FimH is required to initiate the assembly of type 1 pili and thus fimH mutants are nonpiliated [31]. The expression of type 1 pili is under the transcriptional control of an invertible promoter element that governs phase OFF and phase ON populations [32,33]. Interestingly, mutations that inactivate FimH such as the Q133K mutation, bias the fim promoter towards the phase OFF state [34]. Thus, compared to the wild type H10407 strain, or pfimH complemented mutants, isogenic fimH mutants or those complemented with pQ133K, which encodes FimH with a mutation in the mannose binding site [35], were nonpiliated and incapable of yeast agglutination, a phenotypic assay for expression of type 1 pili [36] (Fig 1C) and only the wild type and fimH complemented mutants exhibited demonstrable type 1 pili expression detected by anti-type 1 pili antibodies in immunoblots (Fig 1D). Next, using polarized cultured intestinal epithelia (CIE) derived from the C2BBe1 clone of Caco-2 cells which produce apical brush borders with defined microvilli similar to human intestinal enterocytes [37], we demonstrated that production of FimH was required for effective adhesion. Mutants lacking fimH were significantly less adherent than wild type ETEC (p<0. 0001) (Fig 1E and 1F), while episomal expression of the fimH gene (pfimH), but not the mutant fimH allele (pQ133K), restored adhesion. Additionally, H10407-fimH: Q133K, which contains the Q133K mutant allele of fimH in the chromosome, abrogated yeast agglutination activity and demonstrated significant decrease in adhesion to intestinal cells (S1A Fig). Similarly, mutants lacking the fimA gene encoding the major type 1 pili pilin subunit exhibited loss of functional type 1 pili in yeast agglutination assays, resulting in significant reduction of adhesion to the CIE (S1B Fig). We found that methyl-α-D-mannose but not the methyl-α-D-galactose control sugar, inhibited FimH mediated yeast agglutination by ETEC (Fig 2A) and ETEC adhesion to epithelial cells (Fig 2C). Similarly, antibodies generated against the lectin domain of FimH (α-FimH), but not control antibodies, separated from pre-immune sera, inhibited FimH mediated yeast agglutination with wild type bacteria expressing type 1 pili (Fig 2B) and significantly inhibited ETEC adhesion to intestinal cells (Fig 2D). Collectively, these data support the idea that ETEC utilize type 1 pili to engage intestinal epithelia. We next examined the ability of the FimH tip adhesin to directly engage the intestinal epithelial surface. The purified lectin domain of FimH (FimHLD, 17 kD), representing amino acid residues 1–154 of mature FimH, bound to the apical surface of the CIE. In contrast, binding of FimHLD: Q133K, which lacks mannose binding activity, was markedly diminished (Fig 3A and 3B) as was binding of the wild type FimHLD protein in the presence of exogenous mannose (Fig 3B). ETEC are noninvasive luminal pathogens thought to engage the microvilli at the apical surface of intestinal epithelial cells. Presumably, the FimH lectin can promote this engagement by interacting with mannosylated glycoconjugates on the glycocalyx covering the microvilli. Indeed, using transmission electron microscopy, we identified FimH by immunogold labeling at the ETEC-microvillus interface (Fig 3C). Because FimH interacts with mannosylated receptors on the epithelial surface, we examined the impact of enhanced glycoprotein mannosylation on ETEC pathogen host interactions. Following CIE treatment with kifunensine, an α-mannosidase class 1 enzyme inhibitor which enhances the display of high-mannose glycoproteins [38], we observed a significant increase in FimHLD binding (Fig 4A and 4B). Likewise, WT ETEC adhesion to the kifunensine treated CIE was enhanced relative to the untreated control CIE (p<0. 0001) (Fig 4C and 4D). However, kifunensine treatment had no impact on adhesion of the fimH mutant. These data further suggested that availability of mannosylated glycoproteins on the host cell surface promotes ETEC adhesion through FimH. Because ETEC H10407 expresses both type 1 pili and plasmid encoded CFA/I fimbriae specific to the ETEC pathovar, we investigated whether these CUP structures acted cooperatively in facilitating adhesion to intestinal epithelia. The CFA/I fimbriae are encoded by the cfaABCE operon in which cfaE encodes the CfaE tip adhesin [26,39,40]. Interestingly, mutations in either fimH or cfaE significantly reduced adherence of ETEC compared to wild type H10407, suggesting that both pili participate in ETEC adhesion (S2 Fig). Mutants lacking both the type 1 pili and the CFA/I tip adhesin genes (fimH-cfaE) demonstrated further reduction in adhesion compared to either of the single mutants (S2 Fig). Although additional data are needed to define the precise respective contributions of the chromosomally encoded type 1 pili and the plasmid encoded pathovar specific CFA/I, these initial data support our hypothesis that the structures act in concert to facilitate adhesion. While the CIE used in this study possess some features of normal intestinal epithelial cells, they are derived from metastatic colon cancer cells which fail to represent the diversity of cell types present in intestinal epithelium. Enteroids, derived from human intestinal stem cells collected from healthy volunteers [41–43], can recapitulate many aspects of normal physiology and preserve features of human intestinal epithelium. These include presentation of different cell types including enterocytes, goblet cells, Paneth cells, and endocrine cells [44–46]. Therefore, to further assess the contribution of type 1 pili in ETEC adhesion to intestinal epithelia we established polarized intestinal epithelial monolayers derived from ileal specimens obtained from normal adult human subjects. In these enteroid-derived monolayers we were able to identify enterocytes with a defined brush border and distinct microvilli on the apical surface as well as goblet cells, and chromogranin A positive cells suggesting that they faithfully reproduce many features of surfaces normally presented to bacteria within the intestine (Fig 5A–5E). Similar to our studies of CIE, we found that wild type ETEC adhered to the surface of stem cell derived polarized small intestinal (ileal) monolayers (Fig 6A and 6B) in close approximation with the microvillus surface (Fig 6C), and that mutation of fimH significantly attenuated adherence relative to the wild type parent strain (Fig 6D). Wild type bacteria adhered well to polarized monolayers of enteroid collected from multiple individuals, while the fimH mutant was persistently deficient in its ability to adhere to all target epithelia relative to the parent strain (Fig 6E). These studies suggested that type 1 pili of ETEC potentially play an important role in specifically directing bacterial interaction with the small intestine where release of toxins is thought to provoke the efflux of water and salt that lead to diarrhea. Close contact of ETEC with target epithelial cells is essential for efficient delivery of its enterotoxins [47,48]. To investigate the impact of type 1 pili mediated ETEC-host interactions on toxin delivery, we measured the intracellular production of cAMP and cGMP, cyclic nucleotide second messenger markers for delivery of LT and ST, respectively, in infected cells. cAMP and cGMP was significantly increased in target cells infected with WT H10407 relative to fimH or fimA mutants (Fig 7A and 7B). Methyl-α-D-mannose significantly reduced levels of intracellular cGMP and inhibited cAMP activation in target cells infected with WT H10407 (Fig 7A and 7B). These effects were observed despite wild type levels of heat-labile toxin being produced by the mutants (Fig 7C). Together, these data suggested that type 1 pili are required for optimal delivery of both heat-labile and heat-stable enterotoxins to the target epithelial cells. We next examined the contribution of type 1 pili to pathogenesis in the rabbit ileal loop model, a classical model of virulence for V. cholerae and ETEC [49]. Both fimH and fimA mutants were significantly less adherent than the WT H10407 to rabbit ileal intestinal epithelium (Fig 8A and 8B). Likewise, while considerable fluid accumulation, a hallmark of ETEC virulence, was observed in loops infected with WT H10407 (Fig 8C) no demonstrable fluid accumulation was observed in control loops infected with eltAB mutants [47] which do not make LT toxin (S1 Table), or those containing only PBS, and we detected significantly less fluid accumulation in the loops infected with either the fimH or fimA mutants (Fig 8C), suggesting that type 1 pili mediated pathogen-host interactions contribute to ETEC virulence in this model. To investigate the prevalence of functional type 1 pili expression in clinical ETEC isolates, we tested 174 geographically and phylogenetically disparate clinical isolates including recently sequenced [50] strains using yeast agglutination assays. Overall, the majority (76%) of the clinical isolates demonstrated yeast agglutination activity indicative of preserved functional type 1 pili expression among ETEC. Importantly, we observed type 1 pili expression in isolates possessing each of the major colonization factors (CFs), and in 82% of isolates without any recognizable CFs (Table 1). Overall, these data suggest that functional type 1 pili are highly conserved in diverse ETEC clinical isolates. Collectively data presented here provide evidence that type 1 pili play an essential role in the pathogenesis of these highly diverse pathogens. Similar to their well-established role in the pathogenesis of uropathogenic E. coli (UPEC) [51] where type 1 pili are required for interaction with bladder epithelia, these structures appear to be highly conserved in clinical isolates and appear to be critical for ETEC adhesion and effective engagement of host intestinal epithelia that are ultimately required for efficient delivery of effector molecules including the known toxins.
Enterotoxigenic E. coli are a remarkably diverse group of pathogens that share plasmid-encoded effector molecules, namely heat-labile toxin (LT) and/or heat-stable toxins (ST). In effect, ETEC pathogenesis can be summarized by the virulence features that collectively facilitate the delivery of these toxins [52]. Successful engagement of the complex landscape presented by the intestinal mucosae that includes a secreted mucus layer as well as the glycocalyx, glycoconjugates on the apical surface of the epithelium [53,54], represents an essential step in ETEC virulence. Like other enteric pathogens, ETEC appear to employ a number of different adhesins (lectins) that recognize specific carbohydrate moieties on intestinal epithelia [55–58]. While most studies of ETEC adhesion, and consequently vaccine development, had previously focused on plasmid-encoded colonization factors, recent studies have suggested that bacterial adhesion, intestinal colonization, and toxin delivery ultimately represent very complex phenotypes involving the orchestrated deployment of a variety of pathovar specific plasmid encoded adhesins [14,59,60] as well as highly conserved chromosomally-encoded molecules [61] and other virulence factors including mucinases [62,63]. Although a number of early studies had suggested that ETEC have the capacity to make type 1 pili [30,64], their contribution to pathogenesis had not been comprehensively investigated. Here, we demonstrate convincingly that most ETEC make type 1 pili, that these organisms utilize these highly conserved pili to engage model intestinal epithelia, where these interactions are critical for effective delivery of both heat-labile and heat-stable toxins. Additionally, our data demonstrate that type 1 pili act in concert with the plasmid-encoded pathovar specific colonization factors to promote optimal interaction of H10407 with host intestinal epithelial surfaces. The precise interactions between type 1 pili and the host cell surface have not been thoroughly delineated, however, the data included here support the involvement of the minor pilin tip adhesin subunit (FimH) in mannose-dependent engagement of one or more host cell receptors [65–68]. Before the advent of recombinant techniques to construct isogenic deletion mutants, earlier investigations of ETEC H10407-P, a plasmid-cured strain of H10407 which lacks the large virulence plasmid encoding CFA/I colonization factor, Knutton et. al. suggested that type 1 pili mediated ETEC adhesion to human intestinal biopsies in a fashion that was inhibited by exogenous mannose [28,29]. However, it was suggested that these pili were mediating adhesion to the basolateral surface of enterocytes rather than the apical side [28]. The data presented here overcome many of the technological limitations inherent in these earlier studies and demonstrate convincingly that type 1 pili mediate adhesion of ETEC to apical surface of small intestinal enterocytes, where LT and ST bind to surface GM-1 and guanylate cyclase C receptors, respectively. The present studies raise important questions regarding the nature of type 1 pilus production by ETEC including how these structures might work cooperatively with canonical colonization factor fimbriae, and whether ETEC might encode FimH variants[69,70] that favor colonization of the small intestine. Addressing these issues could be relevant to understanding how type 1 pili might be targeted in vaccines. While ETEC cause a tremendous burden of disease in low-middle income countries, and among travelers to these regions, at present, there is no suitable broadly protective vaccine to prevent infections caused by ETEC. This in part relates to substantial genetic and antigenic heterogeneity within the enterotoxigenic Escherichia coli pathovar. Most vaccines to date, in some form, have targeted the plasmid encoded colonization factor antigens. The remarkable heterogeneity of these antigens [71] and the fact that many ETEC [72,73], more than half of isolates in some studies [74], do not make a recognizable colonization factor have prompted further investigation of these pathogens to define additional vaccine target antigens [75]. Our observation that ETEC isolates from a phylogenetically and geographically diverse collection of strains express functional type 1 pili can potentially inform alternative approaches to design of broadly protective immunogens. In summary, the data presented here demonstrate that ETEC utilize type 1 pili for optimal engagement of the host intestinal epithelium and that these interactions facilitate toxin delivery to the target enterocytes essential for virulence. These studies provide an expanded view of ETEC molecular pathogenesis beyond the canonical paradigm envisioned more than 40 years ago, and potentially afford new avenues for the rational design of strategies to prevent the global burden of disease associated with these important pathogens.
Isogenic fimH and fimA mutants (S1 Table) were constructed using lambda red mediated recombination as previously described [76]. To construct the fimH mutant primers jf101413. 7 and jf101413. 8 (S2 Table) were used to amplify the kanamycin resistance cassette from pKD4 plasmid with 60-bp tails corresponding to the DNA sequence immediately upstream and downstream of fimH. The resulting amplicon was then introduced into H10407 carrying the pKD46 helper plasmid for lambda red-mediated homologous recombination and mutants were selected on 50 μg/ml Kanamycin containing LB-agar plate, and tested for loss of fimH gene by PCR using primers jf120913. 9 and jf120913. 10. A complementation plasmid (pfimH) was constructed by amplifying the fimH gene with its native stop codon using primers jf120814. 1 and jf120814. 2 and cloning into pFLAG-CTC plasmid using infusion cloning kit (Clontech, Takara Bio, USA). A fimA complementation plasmid was constructed by cloning the fimA gene from H10407 into the EcoR1 and BamH1 sites of pTrc99A[77]. Site-directed mutagenesis of pfimH with primers jf031814. 1 and jf031814. 2 was used to change the CAA codon corresponding to the glutamine residue at position 133 of FimH to AAA codon corresponding to the lysine residue resulting in pQ133K (QuikChange, Stratagene, USA). Plasmids pfimH and pQ133K (S3 Table) were then introduced into the fimH mutants for complementation. All mutants were checked for motility, growth and secretion of known effector molecules including LT, EtpA and EatA. To construct an expression plasmid for polyhistidine tagged FimH lectin domain (FimHLD), we amplified the N-terminal lectin region of fimH gene using primers jf042314. 1 and jf042314. 2. The amplicon was then cloned into pETDUET1 (In-Fusion, Clontech). Site directed mutagenesis was used to generate the mannose binding deficient FimHLD: Q133K as described above. The resulting plasmids, pfimHLD and pfimHLD: Q133K, were then introduced into BL21 (DE3) pLys strain for expression and purification of polyhistidine-tagged FimHLD and FimHLD: Q133K. Overnight cultures of BL21 (DE3) pLys containing pfimHLD or pfimHLD: Q133K were diluted 1: 100 into 2 liters of terrific broth supplemented with 100 μg/ml ampicillin and grown for 3 h at 37°C to optical density of ~0. 7 at a wavelength of 600 nm (OD600) then induced with 1 mM IPTG for 3 h at 30°C. Cells were harvested, lysed and his-tagged proteins were purified from the bacterial lysates by nickel affinity chromatography using HisTrap HP column (GE healthcare bioscience, PA, USA). His-tagged FimHLD and FimHLD: Q133K were further purified by size exclusion column chromatography using HiLoad16/600 Superdex 200 pg column (GE). Anti-FimH polyclonal rabbit antiserum was produced against the lectin domain of FimH as previously described [61]. Briefly, two New Zealand White rabbits were immunized (Rockland, USA) with recombinant polyhistidine-tagged FimHLD. Antibodies were separated from serum components using HiTrap columns prepacked with protein G Sepharose (GE). The resulting polyclonal antibodies were then pre-absorbed using lyophilized strain AAEC191-A [15], and affinity purification of antibody against FimHLD immobilized on nitrocellulose was performed as previously described [61,78]. All experiments were carried out using prototypical ETEC strain H10407[79] or the isogenic mutants (S2 Table). Bacteria were grown at type 1 pili inducing conditions by following static incubation for 24 h at 37°C followed by subculturing at 1: 100 for additional 24 h statically at 37°C [34] in LB media supplemented with or without antibiotic, as appropriate, unless otherwise stated. C2BBe1 cells (ATCC Accession Number CRL-2102), a subclone of the Caco-2 cell colonic adenocarcinoma line, was used for generation of the cultured intestinal epithelium (CIE). In order to generate polarized monolayers which form an apical brush border, with microvilli morphologically comparable to that of the human intestinal epithelium [37], we seeded ~2 x105 C2BBe1 cells onto Transwell filters (0. 4μM polystyrene membrane, 6. 5mm diameter insert) in DMEM media supplemented with 10% FBS and 10 μg/ml human transferrin (Lonza, MD, USA) and grew at 37°C with 5% CO2 for three weeks for CIE (polarized monolayer culture). Media were changed every 2–3 days. Formation of microvilli was verified by transmission electron microscopy. Caco-2 cells (ATCC) were cultured in MEM media supplemented with 20% FBS. T-84 (ATCC) cells were cultured in DMEM/F12 (1: 1) media supplemented with 5% FBS. Strains grown under type 1 pili inducing conditions were processed for flow cytometric analysis. For surface staining, bacterial pellets were washed once with PBS, fixed in 2% paraformaldehyde for 15 min and then incubated with 1%BSA in PBS for 30 min at RT. Bacteria were then incubated with primary antibody (α-FimH) at RT for 45 min. After washing with PBS, secondary antibody staining was performed at RT in the dark with species-specific antibodies conjugated with AlexaFluor 546 for an additional 45 min. Bacteria were then washed with PBS and resuspended in 100 μl of PBS for acquisition by flow cytometry (FACSCalibur, BD Biosciences). A minimum of 50,000 organism counts were acquired (CellQuest software, Becton Dickinson), and subjected to subsequent analysis (FlowJo, v7. 6. 3). Type 1 pili were extracted by following a previously described method with some modifications [80]. Briefly, bacteria grown in type 1 pili inducing conditions were harvested, re-suspended in 1 ml of 1 mM Tris-HCl (pH 8. 0) and incubated at 65°C for 1 h with occasional vortexing and pelleted by centrifugation (15,000 × g for 5 min). The supernatant was then transferred to another tube, and an aliquot was precipitated in salt (300 mM NaCl and 100 mM MgCl2) by incubating overnight at 4°C, followed by centrifugation at 20,000 × g for 10 min at 4°C. The protein pellet was re-suspended in 1 mM Tris-HCl (pH 8. 0). Fimbrial extracts were separated by sodium dodecyl sulfate (SDS) -15% polyacrylamide gel electrophoresis minigels (Bio-Rad). Proteins were either stained with Coomassie brilliant blue or transferred to nitrocellulose membranes (Bio-Rad) using a Mini Trans-Blot electrophoretic cell (Bio-Rad) for 60 min at 100 V. The membrane was blocked with 5% milk-PBS supplemented with 0. 05% Tween 20 (Pierce). Incubations with primary (1: 5,000) and secondary (1: 5,000) antibodies were carried out for 1 h at RT. Chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad) was used for detection. Yeast agglutination as a phenotypic test for the production of type 1 pili [36], was performed using the following conditions: strains to be tested were grown in 1 ml Luria broth at type 1 pili inducing conditions, harvested and adjusted to OD600 of 1. 0 in PBS. Agglutination was performed on glass slides by mixing 20 μl bacteria with an equal volume of bakers’ yeast suspension adjusted to OD600 of 1 in PBS. For adhesion assays, bacteria to be tested were grown at type 1 pili inducing conditions and diluted at 1: 10 in pre-warmed cell culture media prior to infection. Serial dilutions of each inoculum were plated on Luria agar for CFU count. Infected CIE were incubated at 37°C and 5% CO2 for 1 h, washed with pre-warmed tissue culture medium 3 times with gentle shaking (100 rpm) for 1 min each. The CIE were then either lysed in 0. 1% Triton X-100 for 5 min, and the cell associated bacteria were recovered by plating lysates onto Luria agar, or processed for fluorescence microscopy for enumeration of bacteria attached to the cells. To investigate the binding of FimH in the context of intestinal epithelial cells, 100 μl of 50 μg/ml biotinylated FimHLD or FimHLD: Q133K were incubated with CIE. After an hour of incubation at 37°C unbound FimHLD were washed off with tissue culture media, the cells were fixed with 2% paraformaldehyde for 30 min at room temperature (RT), washed twice with PBS, and blocked with 1% BSA-PBS for another 30 min at RT. Streptavidin coated Qdot 594 was then used at 1: 100 dilution in 1% BSA-PBS to detect biotinylated FimHLD bound to the cell surface. For detection of ETEC adhesion, infected CIEs were incubated with anti-O78 antibody followed by fluorescent labeled secondary. Cell membranes and nuclei were stained as previously described (CellMask, red for membrane and DAPI for nuclei; Invitrogen) [61]. Images were acquired on a Zeiss LSM510 confocal microscope, and files were converted to TIFF image using ImageJ (v1. 45). Signals were quantified using Volocity software (version 6. 2; PerkinElmer, Inc.). For localization of FimH on intestinal epithelium, ETEC infected CIE were fixed in 2% paraformaldehyde/0. 02% glutaraldehyde (Polysciences Inc. , Warrington, PA) in 100 mM PIPES/0. 5 mM MgCl2, pH 7. 2 for 1 h at 4°C. Samples were washed with PIPES buffer, blocked with 5% Fetal Bovine Serum /5% Normal Goat Serum for 20 min and subsequently incubated with rabbit anti-FimH antibody for 1 h, followed by secondary goat anti-rabbit antibody conjugated to 18 nm colloidal gold for 1 h. Samples were washed in buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h. Samples were then rinsed extensively in dH2O prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc. , Redding, CA) for 1 h. Following several rinses in dH2O, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc. , Bannockburn, IL), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc. , Peabody, MA) equipped with an AMT 8 megapixel digital camera (Advanced Microscopy Techniques, Woburn, MA). Enteroids were grown as previously described in detail [45] from purified intestinal cell lines, maintained at the Washington University Digestive Diseases Research Core Center BioSpecimens Core under a protocol approved by the Institutional Review Board. Briefly, cells were thawed and re-suspended in Matrigel (BD Biosciences, San Jose, CA, 15 μL/well in 24 well plates), and incubated at 37°C with a 1: 1 mixture of L-WRN conditioned media (CM) and primary culture media (Advanced DEM/F12, Invitrogen) supplemented with 20% fetal bovine serum (FBS), 2 mM L-glutamine, 100 units/mL penicillin, 0. 1 mg/mL streptomycin, 10 μM Y-27632 (ROCK inhibitor; Tocris Bioscience, R and D Systems, Minneapolis, MN), and 10 μM SB 431542 (transforming growth factor-ß type 1 receptor inhibitor; Tocris Bioscience, R and D Systems). For polarization, cells were added to Transwell filters (Corning) and incubated in differentiation media (1: 20 mixture of L-WRN CM and primary culture media) lacking SB 431542. Cells were grown to confluence for 3 days in differentiation media before use. For histological analysis (Day 3), enteroids grown on transwell were fixed in 3. 7% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 15 min, washed once with PBS and processed for paraffin embedding. Transverse sections (5 μm) were stained with hematoxylin and eosin (visualized with a Zeiss Axioskop 2 MOT microscope fitted with a CRI Nuance FX multispectral imaging system, Cambridge Research and Instrumentation), or used for immunostaining. For immunostaining, sections were de-paraffinized and hydrated, boiled in Unmasking Solution (Vector Laboratories, Burlingame, CA) for 25 min, rinsed in PBS, blocked in 1% bovine serum albumin/0. 1% Triton-X100 for 30 min and incubated with primary antibody at 4°C overnight. Primary antibodies included rabbit anti- ChgA (1: 100, Abcam, Cambridge, MA), rabbit anti-Muc2 (1: 100, Santa Cruz Biotechnology, Inc. , Dallas, Texas), mouse monoclonal anti-Villin 1 (1: 100; Santa Cruz Biotechnology, Inc.). Slides were rinsed 3 times with PBS and incubated for 60 min at RT with species specific secondary antibodies (1: 200; Invitrogen) conjugated to AlexaFluor488 or AlexaFluor546. For detection of bacteria, enteroids (grown for 3 days) were infected for 1 h, washed 3 times with pre-warmed media, fixed in 3. 7% paraformaldehyde and either processed for fluorescent microscopy or embedded in paraffin. For the detection of tight junction formation, antibody against zonula occludens-1 (anti-ZO-1, mouse monoclonal, Invitrogen) was used at 1: 100 dilutions followed by anti-mouse secondary antibodies conjugated to AlexaFluor488 (1: 200, Invitrogen). Paraffin embedded sections were deparaffinized and processed for immunostaining with anti-O78 antibody (1: 200) followed by secondary antibodies (1: 200; Invitrogen) conjugated to AlexaFluor 546 for the detection of cell associated bacteria. UEA-1 lectin conjugated to FITC (1: 100, Sigma) was applied during secondary antibody incubation. Slides were washed 3 times in PBS and stained with DAPI (Molecular Probe) to visualize nuclei and cellmask red (1: 2000, Invitrogen) to visualize plasma membrane and mounted with ProlongGold antifade reagent (Molecular Probes) for confocal microscopy (Nikon ECLIPSE Ti confocal microscope equipped with NIS-Elements imaging software). To examine ETEC association with microvilli of polarized primary human small intestinal epithelial cells, infected monolayers were processed for transmission electron microscopy following protocol mentioned above. LT toxin secretion by different strains was measured using previously established GM1-ELISA method [81]. Briefly, ELISA plates (Costar, Corning, NY) were coated with 100 μl/well of 1 μg/ml monosialoganglioside GM1 (Sigma, G-7641), and incubated overnight at RT. After blocking with 2% BSA in PBS-Tween, 100 μl of clarified culture supernatant from overnight Luria broth cultures of bacteria was added to wells in triplicate and incubated for 1 h at 37°C. The wells were then washed and incubated with 100 μl of a 1: 5000 dilution of rabbit anti-LTB polyclonal antisera for 1 h. Plates were again washed and then incubated with 100 μl of a 1: 5000 dilution of anti-rabbit secondary conjugated with HRP for another hour. Finally plates were developed with 100 μL per well of TMB-H2O2 (KPL) substrates and read immediately at 630nm for kinetic measurement (Eon, BioTek instruments, VT, USA). Caco-2 cell monolayers grown in 96-well plates were infected with WT or mutant strains and used to determine alterations in cAMP. After 2 h of infection wells were washed with pre-warmed tissue culture media, incubated for an additional 2. 5 h, cells lysed in sample buffer and intracellular cAMP concentrations were then determined by ELISA (cAMP Direct EIA, Arbor Assays, MI, USA). Similarly, T-84 cell monolayers grown in 96-well plates were used to determine cGMP concentrations following infection. 1. 5 h after infection, wells were washed 3 times with pre-warmed tissue culture media, incubated for an additional 3 h, lysed in sample buffer, and intracellular cGMP determined (cGMP Direct EIA, Arbor Assays, MI, USA). The rabbit ileal loop (RIL) assay was performed as previously described [49,82]. Briefly, bacteria were grown under static conditions in Luria broth at 37°C, then washed with PBS, and diluted yield to a target inoculum of ~1X106 CFU/ml/loop. Adult albino rabbits (New Zealand strain) weighing 1. 5–2. 0 kg were fasted for 24 h and allowed only water. Following induction of anesthesia with intravenous sodium pentobarbital (0. 5 mL/kg body mass), the abdominal cavity was entered, the intestine exposed and the ileum localized. Here, loops of 5 cm in length were created with surgical ligatures, with 2 cm intervals between each loop to yield 5–6 loops per rabbit. Loops were then inoculated with either wild type H10407 as a positive control or mutant strains. Animals were euthanized ~18 h post infection with sodium pentobarbital, the intestine exposed and the ileocaecal region was then removed. The volume of fluid accumulation determined per unit length of gut within each segment, after which tissue specimens from the corresponding segment were collected, fixed in formalin (10%), paraffin-embedded and subsequently processed for examination of villous associated bacteria following protocol mentioned above. Rabbit ileal loop protocol conducted at icddr, b was reviewed and approved by the institution’s Animal Experimentation Ethics Committee (AEEC). Experimental procedures were carried out by veterinarians at the animal facility in accordance with relevant guidelines. The rules and guidelines followed at icddr, b for animal care and use adhere to the recommendations, with some modifications, stated in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH). These guidelines and the subsequent modifications were approved by the icddr, b Board of Trustees. All work involving cell lines derived from human biopsy specimens with the Washington University Digestive Diseases Research Core Center BioSpecimens Core was performed under a protocol (201406083) approved by the Institutional Review Board at Washington University School Medicine.
Enterotoxigenic Escherichia coli (ETEC) infections contribute substantially to death and morbidity due to diarrheal illness and are associated with serious sequelae including malnutrition, stunted growth, and intellectual impairment among young children in developing countries. Effective engagement of intestinal epithelial cells is essential for ETEC pathogenicity. Consequently, pathovar specific plasmid-encoded adhesin structures known as colonization factors (CFs) have been a principal target for vaccines. However, tremendous inter-strain variation in the carriage of gene clusters encoding different CFs and significant antigenic diversity of the CF adhesins has posed a challenge to vaccine development. In contrast, type 1 pili are encoded by the fim operon located in the chromosome of most ETEC strains and are highly conserved. While type 1 pili are known to play a critical role in virulence of extraintestinal pathogenic E. coli, the present studies represent the first detailed examination of the contribution of these pili in ETEC pathogenesis. Here we demonstrate that ETEC type 1 pili are essential for optimal interactions with intestinal epithelia and that they play a critical role in virulence. These data may inform additional approaches toward development of broadly protective vaccines for these pathogens of global importance.
bacteriology
medicine and health sciences
toxins
pathology and laboratory medicine
pathogens
microbiology
pili and fimbriae
toxic agents
toxicology
epithelial cells
cellular structures and organelles
bacterial pathogens
digestive system
adhesins
microbial physiology
animal cells
proteins
medical microbiology
microbial pathogens
biological tissue
pathogenesis
pathogen motility
gastrointestinal tract
biochemistry
bacterial physiology
lectins
cell biology
anatomy
virulence factors
epithelium
biology and life sciences
cellular types
Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions