Dendritic cells (DCs) are phagocytes that are highly specialized for antigen presentation. Heterogeneous populations of macrophages and DCs form a phagocyte network inside the red pulp (RP) of the spleen, which is a major site for the control of blood-borne infections such as malaria. However, the dynamics of splenic DCs during Plasmodium infections are poorly understood, limiting our knowledge regarding their protective role in malaria. Here, we used in vivo experimental approaches that enabled us to deplete or visualize DCs in order to clarify these issues. To elucidate the roles of DCs and marginal zone macrophages in the protection against blood-stage malaria, we infected DTx (diphtheria toxin) -treated C57BL/6. CD11c-DTR mice, as well as C57BL/6 mice treated with low doses of clodronate liposomes (ClLip), with Plasmodium chabaudi AS (Pc) parasites. The first evidence suggesting that DCs could contribute directly to parasite clearance was an early effect of the DTx treatment, but not of the ClLip treatment, in parasitemia control. DCs were also required for CD4+ T cell responses during infection. The phagocytosis of infected red blood cells (iRBCs) by splenic DCs was analyzed by confocal intravital microscopy, as well as by flow cytometry and immunofluorescence, at three distinct phases of Pc malaria: at the first encounter, at pre-crisis concomitant with parasitemia growth and at crisis when the parasitemia decline coincides with spleen closure. In vivo and ex vivo imaging of the spleen revealed that DCs actively phagocytize iRBCs and interact with CD4+ T cells both in T cell-rich areas and in the RP. Subcapsular RP DCs were highly efficient in the recognition and capture of iRBCs during pre-crisis, while complete DC maturation was only achieved during crisis. These findings indicate that, beyond their classical role in antigen presentation, DCs also contribute to the direct elimination of iRBCs during acute Plasmodium infection.
The spleen is a primary site for the control of blood-borne infectious diseases in humans and rodents [1], [2]. Although splenic phagocytic activity has been well documented in vitro[3]-[5] and ex vivo [3], [6]-[8], few studies have reported on the in vivo three-dimensional (3D) interactions between splenic phagocytes and pathogens [9]. Addressing this issue is particularly important in the case of malaria, a disease characterized by splenic involvement that is critical for controlling blood-stage Plasmodium parasites [10]. In recent years, confocal intravital microscopy (CIVM) [11] has been used to study host-pathogen interactions during infectious diseases caused by viruses [12], [13], bacteria [14] and protozoan parasites [15]. For example, CIVM revealed important aspects of the Plasmodium life cycle [16], [17]. Other works described Plasmodium-induced immune responses inside the placenta [18] and the dermis using fluorescent stereomicroscopy [19]. A single publication reported the movements of Plasmodium-infected red blood cells (iRBCs) inside the spleen [20]. However, no in vivo study has addressed the interactions between blood-stage Plasmodium parasites and the splenic immune system. Splenectomized patients with acute Plasmodium falciparum infections have an impaired ability to remove parasites from circulation [21], similar to splenectomized mice infected with the blood-stages of Plasmodium chabaudi (Pc) [22]. In humans and mice, the phagocytosis of iRBCs or free merozoites by splenic phagocytes begins soon after infection and helps to control the parasitemia and induce the lymphocyte response [23], [24]. This occurs primarily inside the red pulp (RP) and the marginal zone (MZ) of the spleen [23], [24], where a complex phagocyte network is formed by heterogeneous populations of macrophages and dendritic cells (DCs) [25], [26]. In an effort to characterize the role of splenic phagocytes in Pc malaria, a recent study identified migrating monocytes as major participants in the clearance of iRBCs [8]. However, previous studies that quantified the ex vivo phagocytosis of iRBCs by flow cytometry reported low percentages of splenic phagocytes containing Pc remnants [8], [27]. This observation is not fully compatible with the notion that the role of the spleen is of the utmost importance in parasite control. DCs are phagocytes that are highly specialized in presenting antigens to T cells [28]. Splenic DCs are efficient antigen presenting cells (APCs) during the massive T and B cell responses to acute Pc malaria [29]-[31]. Within the first week of Pc infection, splenic DCs up-regulate the expression of major histocompatibility complex (MHC) and costimulatory molecules, secrete pro-inflammatory cytokines, and stimulate T cell proliferation and IFN-γ production [32]-[34]. Nevertheless, it is still unclear whether DCs are unique in their ability to initiate CD4+ T cell responses to Pc blood-stages in the spleen, as observed in Plasmodium berghei (Pb) malaria [35]. Moreover, many details concerning the dynamics of splenic DCs in malaria remain unknown, limiting our understanding of the involvement of these cells in the protective immune response. After taking on antigens, immature DCs lose the ability to phagocytize and migrate towards T cell-rich areas to initiate the adaptive immune response [28]. Thus, it would be expected that DCs leave the RP soon after phagocytizing iRBCs or free merozoites and no longer contribute to parasite clearance, although this is as yet only a supposition. In this study, we took advantage of experimental approaches that enabled us to deplete or visualize splenic DCs in vivo to clarify these issues. The in vivo depletion of phagocytes clearly demonstrated that DCs are key participants in the early control of the blood stage of infection with Pc and Plasmodium yoelii (Py) iRBCs, as well as the blood stage of infection with Pb sporozoites. The phagocytosis of Pc iRBCs by splenic DCs was analyzed by CIVM, as well as by flow cytometry and immunofluorescence, in three distinct situations: at the first encounter, at a pre-crisis phase concomitant with parasitemia growth and at a crisis phase, when parasitemia has dramatically dropped and changes in the splenic architecture have culminated in spleen closure [36]. CIVM allowed us to visualize the phagocytosis of Pc iRBCs by the RP DC network, the movement dynamics and morphological changes of DCs and the interaction between DCs and CD4+ T cells at the different phases of acute Pc malaria. To our knowledge, this is the first description of the in vivo interaction between Plasmodium iRBCs and the splenic immune system.
To evaluate whether DCs are important for the early control of blood-stage Pc malaria, C57BL/6. CD11c-DTR (B6. CD11c-DTR) mice were treated with diphtheria toxin (DTx). The great majority of splenic CD11c+I-A+ cells were eliminated in DTx-treated B6. CD11c-DTR mice (Fig. 1A). No effect was observed on F4/80+ RP macrophages, but the already small population of MARCO/MOMA-1+ MZ macrophages was depleted (S1 Fig.). Starting in the earliest days of infection, DTx-treated B6. CD11c-DTR mice had higher parasitemia (Fig. 1B) and weight loss (Fig. 1C) in comparison to their PBS-treated counterparts, leading to an accumulated mortality of 75% of mice on day 15 p. i. (Fig. 1D). On day 4 p. i. , DTx-treated B6. CD11c-DTR mice had reduced numbers of CD4+ T cells per spleen (Fig. 1E). DTx treatment also completely abrogated the CD4+ T cell proliferation and IFN-γ production in vitro in response to iRBCs (Fig. 1F). None of these effects were observed in DTx-treated C57BL/6 (B6) mice (Figs. 1 and S1). Furthermore, the selective elimination of MZ macrophages by treating B6 mice with a low dose of clodronate liposomes (ClLip) did not affect the course of parasitemia, IFN-γ production by splenic CD4+ T cells or mouse survival (S2 Fig.). Similarly to what was observed for the Pc parasite, DTx treatment in B6. CD11c-DTR mice exacerbated Py malaria from the beginning of infection (S3A–S3C Fig.). The role of DCs in the early control of parasitemia was also evaluated in B6 and B6. CD11c-DTR mice that were treated with DTx on day 2 p. i. with Pb sporozoites. DTx-treated B6. CD11c-DTR mice presented with higher parasitemias (S3D–S3E Fig.). In this case, however, DTx treatment prolonged the survival of infected B6. CD11c-DTR mice by protecting them from cerebral malaria (S3F Fig.). To investigate whether splenic DCs phagocytize iRBCs in recently infected mice, we analyzed the interaction between YFP+ cells and mCherry-Pc iRBCs in the subcapsular RP of C57BL/6. CD11c-YFP (B6. CD11c-YFP) mice using CIVM [26]. Mice were infected by i. v. administration of mature iRBCs (>95% late trophozoites/schizonts), as these cells are known to be recognized and phagocytized by DCs [37]. In naïve mice, YFP+ cells were non-motile and actively extended protrusions and dendrites (S1 Video). At 15 min p. i. , mCherry-Pc iRBCs were present in the subcapsular RP (Fig. 2A, S2 Video). CIVM 3D animations showed mCherry-Pc iRBC remnants inside YFP+ cells (yellow spots of merged mCherry/YFP-3D signal; Fig. 2B, S3 Video). At this time, 16% of YFP+ cells contained mCherry-Pc fragments (Fig. 2C). We also observed several mCherry-Pc iRBCs trapped by YFP+ cells without visible signs of internalization (Fig. 2A, S4 Video). Thus, a substantial proportion of subcapsular RP YFP+ cells trapped or internalized iRBCs soon after Pc infection. These cells were not activated, as indicated by small YFP+ cell volume and sphericity (Fig. 2D). The phagocytic activity of splenic DCs from recently infected B6 mice was also analyzed ex vivo by immunofluorescence and flow cytometry. Immunofluorescence revealed approximately 5% CD11c pixels that were colocalized with GFP pixels in those spleens (Fig. 3A and 3B). The majority of GFP-Pc iRBCs were trapped inside the RP and MZ (Fig. 3B). Nearly 2% of CD11c+ cells internalized Cell Tracer Violet (CTV) -Pc parasites (4 × 104 CTV+CD11c+ cells/spleen), as revealed by flow cytometry (Fig. 3C). Comparable data were obtained with Green Fluorescent Protein (GFP) -Pc iRBCs (S1 Table). This phagocytic activity was not restricted to a DC subtype, as subsets of CD11c+ cells co-expressing CD11b, CD8, B220 or CD4 were CTV+ (S4A Fig.). Considering the numbers of cells per spleen, CD11b+CD11c+ cells were responsible for most of the parasite clearance carried out by CD11c+ cells in recently infected mice (S4B Fig.). Although 61% of YFP+ cells in recently infected B6. CD11c-YFP mice had a DC phenotype, expressing CD11c and MHC class II (I-A) but not F4/80,20% displayed the phenotype of F4/80+ RP macrophages (S5 Fig.). Therefore, we also analyzed the phagocytic activity of the YFP+ cell subsets by CIVM and flow cytometry. With injection of a fluorescent anti-F4/80 mAb into mice, CIVM revealed that 17% of cells in the subcapsular RP YFP+ cell population were F4/80+ soon after infection (Fig. 2E and 2F, S5 Video). Approximately 15% of F4/80+YFP+ and F4/80-YFP+ cells internalized Cell Tracker Red CMTPX (CMTPX) -Pc parasites (Fig. 2G), but only 20% of the CMTPX+YFP+ cells were F4/80+ (Fig. 2H). Flow cytometry analysis of the YFP+ cell subsets showed that a proportion of CD11c+ and F4/80+ cells was CTV+ in B6. CD11c-YFP mice that were recently infected with CTV-Pc iRBCs (Fig. 3D). The CD11c+ cells made up 63% of the CTV+YFP+ cell population (4. 5 × 104 CTV+CD11c+YFP+ cells/spleen), while 37% of CTV+YFP+ cells expressed F4/80 (2. 5 × 104 CTV+F4/80+YFP+ cells/spleen) (Fig. 3E and 3F). Next, we evaluated the dynamics of splenic DCs during early Pc malaria. At 12 h p. i. , the subcapsular RP YFP+ cells from B6. CD11c-YFP mice displayed higher speed and displacement (Fig. 4A). This enhanced motility of YFP+ cells correlated with their migration towards CD4+ T cell-rich areas. This was evident in immunofluorescences, at 2 h and 24 h p. i. , by the presence of yellow areas of merged FITC/PE signal (Fig. 4B) and higher percentages of CD11c-CD4 pixel colocalization (Fig. 4C). We also adoptively transferred CD4+ T cells expressing Cyan Fluorescent Protein (CFP) into B6. CD11c-YFP mice to evaluate the interaction of subcapsular RP DCs with CD4+ T cells during early Pc malaria. In naïve mice, most CFP+CD4+ cells made transient contacts with YFP+ cells (Fig. 4D, S6 Video), and CFP+CD4+ cells were actively moving inside spleen (Fig. 4E). At 24 h p. i. , CFP+CD4+ cells contacted YFP+ cells more stably (Fig. 4D, S7 Video), as indicated by a decrease in CFP+CD4+ cell speed and an increase in arrest coefficient (Fig. 4E). To investigate whether splenic DCs have a direct role in parasite clearance during pre-crisis, we analyzed the interactions between splenic DCs and iRBCs after five days of infection in vivo and ex vivo. This possibility was suggested by our data showing that, on day 5 p. i. , splenic DCs had an enhanced expression of the phagocytic receptor FcγRI (S6A–S6B Fig.). Notably, we visualized many mCherry-Pc iRBCs inside the subcapsular RP, and YFP+ cells displayed intense phagocytic activity (Fig. 5A, S8 Video). The presence of intense vacuolization in these DCs was also clear, and we observed some YFP+ cells (containing iRBC remnants from previous internalization events) phagocytizing mCherry-Pc iRBCs (Fig. 5A, S9 Video). CIVM 3D animations confirmed the internalization of mCherry-Pc parasites by YFP+ cells (Fig. 5B, S10 Video). This phenomenon was observed in 45% of the YFP+ cells (Fig. 5C). At five days p. i. , YFP+ cells were activated and displayed higher cell volume and lower cell sphericity than those from recently infected mice (Fig. 5D; S1 Table). On day 5 p. i. , the CD11c+ cells also expressed higher levels of MHC class II, CD80 and CD86 compared to those from naïve mice (S6C–S6D Fig.). Immunofluorescence corroborated the significant role of splenic DCs in the widespread iRBC phagocytosis observed during pre-crisis. The percentages of CD11c pixels that colocalized with GFP pixels reached up to 40% in spleens from B6 mice on day 5 p. i. (Fig. 6A and 6B). Flow cytometry confirmed that splenic DCs were able to phagocytize iRBCs during pre-crisis. When mature CTV-Pc iRBCs were i. v. injected into B6 mice on day 5 p. i. , approximately 4% of splenic DCs were CTV+ (1. 4 × 105 CTV+CD11c+ cells/spleen) (Fig. 6C and 6D). Phagocytic activity was not restricted to a particular DC subtype, as a proportion of all subsets studied internalized iRBCs during pre-crisis (S4A Fig.). However, CTV+ CD11b+CD11c+ and CD8+CD11c+ cell numbers were significantly higher per spleen than those of other DC subsets (S4B Fig.). In addition, on day 5 p. i. , 10% of CD11c+ cells from mice infected with GFP-Pc iRBCs were GFP+ (4 × 105 CTV+CD11c+ cells/spleen) (Fig. 6E and 6F). Comparatively, we observed substantially higher activation and phagocytic activity both in vivo and ex vivo in the splenic DCs during pre-crisis (S1 Table). Furthermore, a significantly higher frequency of iRBC uptake was detected using CIVM in comparison with flow cytometry. Notably, flow cytometry analysis of splenic YFP+ cells from B6. CD11c-YFP mice during pre-crisis showed a sharp reduction in the percentages of F4/80+ cells so that the great majority of the YFP+ cell population presented with a classical DC phenotype (S5 Fig.). Moreover, a large fraction of CD11c+YFP+ cells in these mice expressed higher levels of MHC class II molecules in comparison to those in recently infected B6. CD11c-YFP mice. This observation was confirmed by CIVM, which revealed a reduction of F4/80+YFP+ cells in the subcapsular RP of B6. CD11c-YFP mice on day 5 p. i. (Fig. 5E and 5F, S11 Video). Due to the incremental number of CD11c+ cells in the YFP+ cell population, almost all of the phagocytic activity of YFP+ cells was imputed to DCs during pre-crisis, as observed by CIVM (Fig. 5G and 5H) and by flow cytometry (Fig. 6G, 6H and 6I). During the crisis phase of acute Pc malaria, profound modifications in the splenic architecture occur, resulting in RP closure [36]. Therefore, we extended our study into this phase of the disease. CIVM revealed only occasional mCherry-Pc iRBCs trapped by subcapsular RP YFP+ cells in B6. CD11c-YFP mice on day 8 p. i. (Fig. 7A and 7B, S12 Video), and yellow spots of merged mCherry/YFP-3D signal were infrequent (Fig. 7C). At that same time point, YFP+ cell volumes were smaller than during pre-crisis (Fig. 7D, S1 Table). YFP+ cell sphericity was reduced in mice on days 5 and 8 p. i. compared with naïve mice (Fig. 7D, S1 Table). Flow cytometry also revealed poor phagocytosis by splenic DCs, a process that was investigated both when mice were re-infected i. v. with mature CTV-Pc iRBCs and when mice were i. p. infected with GFP-Pc iRBCs (Fig. 7E, 7F, 7G and 7H). These data indicate that splenic DCs could be primarily involved in antigen presentation rather than in phagocytosis during crisis, as CD11c+ cells expressed high levels of MHC class II and CD80 on day 8 p. i. (S6C–S6D Fig.).
The depletion of phagocytes in vivo allowed us to clearly demonstrate the key role of DCs in the protection against experimental blood-stage malaria. Abundant CD11c expression is a well-known marker for DCs, which are primary targets of DTx treatment in B6. CD11c-DTR mice [38]. Nevertheless, MZ macrophages are also depleted in DTx-treated B6. CD11c-DTR mice due to ectopic expression of the DTx receptor transgene [39]. The role of DCs was established in our study by comparing the disease progression in DTx-treated B6. CD11c-DTR mice and in B6 mice treated with a low dose of ClLip, which selectively depletes MZ macrophages within splenic phagocytes [39], [40]. The significant contribution of DCs in the control of Pc malaria was suggested by data showing the worsening of the disease in DTx-treated B6. CD11c-DTR mice, while the elimination of MZ macrophages by the ClLip treatment did not alter the course of infection in B6 mice. Our data also showed that splenic DCs are required for CD4+ T cell proliferation and IFN-γ production during Pc infection. The complete abrogation of these responses in DTx-treated B6. CD11c-DTR mice, but not in ClLip-treated B6 mice, demonstrated that other splenic phagocytes such as MZ and RP macrophages did not replace DCs in the initiation of CD4+ T cell responses to Pc infection. Our first evidence suggesting that DCs could directly contribute to parasite clearance was the effect of DC depletion on the increase of parasitemia and the reduction of body weight during the first days of blood-stage Pc and Py malaria. DCs were also required to control the early parasitemia following infection with Pb sporozoites. The early protective role of DCs could not be completely attributed to the need for these cells to activate T cells, which take longer to produce IFN-γ and induce antibody secretion during experimental malaria. The splenocytes obtained four and five days after Pc infection still require further stimulation with iRBCs in vitro to differentiate into effector cells [41], [42], while the ex vivo production of IFN-γ and antibodies coincides with the drop of parasitemia a week after infection [42], [43]. Using in vivo and ex vivo approaches, we unequivocally demonstrated here that the subcapsular RP DCs recognize and phagocytize mature iRBCs during the first encounter and pre-crisis, while spleen closure coincides with limited Pc phagocytosis by DCs during crisis. Although the splenic DCs are thought to be a major DC population in intimate contact with the bloodstream, these cells may act together with other DCs outside the spleen to clear Plasmodium parasites. This idea is supported by studies in splenectomized mice showing that other reticuloendothelial organs, such as the liver, effectively substitute for the phagocytic functions of the spleen in protecting against Pc malaria [22], [44]. In fact, hepatic CD11c+ DCs are also capable of internalizing iRBCs in the liver sinusoids during acute Pc infection [45]. CIVM allowed us to visualize the interaction between subcapsular RP DCs and iRBCs in great detail. In naïve mice, these cells actively extended protrusions and dendrites, as previously shown [26]. Soon after infection, we observed iRBCs being trapped by DCs that had a non-activated phenotype. The majority of these cells showed a classical DC phenotype, but a proportion of them exhibited strong labeling for F4/80, a marker of RP macrophages that is also expressed by a subset of DCs in the skin [46]. Another study reporting a similar observation concluded that, based on their dendritic morphology, subcapsular RP F4/80+YFP+ cells represent a subset of peripheral tissue DCs [26]. Although we did not visualize phagocytosis of iRBCs in recently infected mice, the detection of Pc remnants inside subcapsular RP DCs suggests that iRBC uptake had occurred. In fact, parasite antigen presentation is likely to occur soon after Pc infection, similar to the process observed during L. monocytogenes infection [47]. During the first day p. i. , subcapsular RP DCs displayed high motility and made stable contacts with CD4+ T cells. DCs also migrated rapidly to T cell-rich areas following Pc infection, a process that might involve chemokine signaling as suggested by studies in CCR7-knockout mice [48]. Here, for the first time, we observed the phagocytosis of iRBCs during pre-crisis in vivo. This occurred in a large number of subcapsular RP DCs, such that up to half of this population presented with Pc remnants. The great majority of these cells had a classical DC phenotype, which was characterized by negative staining for F4/80 and high expression of both MHC class II and costimulatory molecules. It is notable that these cells displayed an activated phenotype. Even if most subcapsular RP DCs during pre-crisis are immature cells that recently migrated to the spleen [49], it is expected that DC activation leads to their maturation and consequent blockade of phagocytic activity, allowing the cellular machinery to be restructured for antigen presentation [28]. In agreement with our data, a previous report determined that the peak of in vitro iRBC uptake by splenic DCs occurred at five days p. i. , in parallel with the increase in the expression of MHC class II and costimulatory molecules [34]. In both studies, the phagocytic activity was not restricted to a particular DC subset. Our ex vivo data implicate CD11b+ and CD8+ DCs in most of the parasite clearance imputed to splenic DCs in mice both soon after infection and at the pre-crisis phase. Consistent with the immune response to acute Pc malaria, the CD11b+ and CD8+ DC subsets are known to be specifically involved in antigen presentation to CD4+ T cells and IL-12 production, respectively [50], [51]. Furthermore, both subsets of DCs are able to induce IFN-γ production by parasite-specific T cells during Pc infection [29]. Another important observation during pre-crisis was a sharp decline in the population of F4/80+YFP+ cells, a phenomenon that also occurred to splenic F4/80+ macrophages after the parasitemia peak (unpublished data). Because DCs have a higher turnover than F4/80+ macrophages [47], a possible explanation for our results is that a proportion of these phagocytes died after ingesting Pc parasites and only DCs were rapidly replaced. This process would substitute F4/80+ macrophages, a resident RP population that is primarily required to maintain tissue homeostasis [52], to inflammatory phagocytes. An alternative explanation is the down-regulation of the F4/80 molecule due to macrophage activation as reported during mycobacterial infection [53]. The F4/80+YFP+ cells could also have migrated to other locations such as the splenic T cell-rich areas. During crisis, the down-regulation of the phagocytic function of splenic DCs coincided with the period of spleen closure. This was demonstrated here by in vivo images showing a few iRBCs in the subcapsular RP at eight days p. i. , when parasitemias were even higher than at five days p. i. . The decline in iRBC uptake was also associated with the maximum expression of MHC class II and CD80 molecules by splenic DCs, which indicates that complete DC maturation was only achieved during crisis. This idea is corroborated by a previous study that reported a decrease to baseline levels of the in vitro uptake of the iRBCs by splenic DCs at day 8 p. i. [34]. Thus, in addition to spleen closure and the subsequent blockade of iRBC entry inside the RP, splenic DCs seem to lose the ability to phagocytize parasites, while concomitantly increasing their ability to present cognate antigens. This is an interesting observation because, during crisis, most of the lymphocytes that are activated during early Pc infection undergo apoptosis [54], [55]. Thus, it is possible that mature DCs are required to expand and differentiate the few remaining T cells, giving rise to the memory response to malaria [56], [57]. The quantification of iRBC phagocytosis ex vivo by flow cytometry yielded substantially lower percentages of Pc+ DCs compared with in vivo data obtained by CIVM. This discrepancy may result from differences in the fluorescence detection thresholds of CIVM and flow cytometry, the DC subpopulations examined by these techniques (subcapsular RP DCs or total splenic DCs, respectively) or the fluorochrome labeling of the iRBCs (mCherry, GFP, CTV or CMTPX). Another possible explanation for the low detection of iRBC uptake by flow cytometry is the rapid iRBC degradation or fluorochrome quenching [8], such that Pc remnants were only identified inside DCs shortly after phagocytosis. Previously, low frequencies of iRBC uptake were also detected by flow cytometry in migrating monocytes [8], [27]. Immunofluorescence confirmed that splenic DCs, particularly those localized inside the RP and MZ, play a major role in the clearance of iRBCs during acute Pc infection. Although this technique did not efficiently discriminate single cells, the percentages of CD11c-GFP pixel co-localization were comparable to those of Pc+ DCs obtained by CIVM. The in vivo approaches used in this study indicate that, beyond the classical role of DCs in antigen presentation, these cells also contribute to the direct elimination of iRBCs during acute Plasmodium infection. For several days after Pc infection, subcapsular RP DCs were highly efficient in the recognition and capture of iRBCs. Complete DC maturation appeared to be achieved only during crisis when restructuring of the spleen might facilitate the development of the acquired immunity. Taking into account the specifics of different parasite-host interactions, we speculate whether our findings in mouse models could be applied to human malaria. The adhesion of P. falciparum iRBCs to human monocyte-derived DCs through the scavenger receptor CD36 has been shown to inhibit DC maturation and subsequently reduce their capacity to activate T cells [58]. This observation was interpreted as the impairment of the DC function during P. falciparum infection. However, our data showing the induction of FcγRI in splenic DCs during pre-crisis open the possibility that recognition of opsonized iRBCs through this receptor can overcome the down-regulatory activity of CD36 signaling. Thus, the opposite effects of malaria on DC function could be related to the different activation profiles of DCs, which are greatly influenced by the surrounding tissue microenvironment, rather than other factors previously discussed such as different species and strains of hosts and parasites [59]. Together, our data add novel information to this area of immunology and demonstrate that in vivo imaging may help to unravel the mechanisms underlying protective immunity against malaria.
Six- to eight-week-old B6, B6. CD11c-DTR [28], B6. CFP [60] and B6. CD11c-YFP mice [61] were bred under specific pathogen-free conditions at the Animal Facilities of Instituto Gulbenkian de Ciência (IGC), Instituto de Ciências Biomédicas at the Universidade de São Paulo (ICB-USP) or Institut de Transgénose Orléans-Villejuif. Pc (AS strain), Py (XL strain) and mCherry-Pc were maintained previously as described [62], [63]. GFP-Pc parasites were selected by treatment with pyrimethamine (Sigma-Aldrich, USA) [64]. The Instituto de Medicina Molecular at the Universidade de Lisboa provided Anopheles stephensi mosquitoes infected with Pb (ANKA strain). Mice were infected intraperitoneally (i. p.) with 1 × 106 iRBCs (blood from infected mice), and intravenously (i. v.) with 1 × 108 iRBCs or 1 × 103 sporozoites. Purified iRBCs were used where specified. The iRBCs were obtained during a period of the circadian cycle in which mature stages predominated (>95% late trophozoites/schizonts). All procedures were in accordance with the national regulations of Conselho Nacional de Saúde and Colégio Brasileiro em Experimentação Animal (COBEA) and Federation of European Laboratory Animal Science Associations (FELASA). The protocols were approved by the Comissão de Ética no Uso de Animais (CEUA) of ICB-USP, São Paulo, Brazil under permit numbers 0036/2007 and 0174/2011, and by FELASA under permit number AO10/2010. To deplete CD11c+ cells, B6. CD11c-DTR mice were injected i. p. with a single dose of 2 ng/g body weight of DTx (Sigma-Aldrich) 24 h before iRBC infection or 48 h after sporozoite infection. This dose is half of the one previously established to deplete CD11c+ cells [65] and it was used to reduce drug toxicity. To deplete MARCO+/MOMA1+ cells, B6 mice were injected i. v. with 8. 5 µg/g body weight of ClLip 24 h before infection [40]. Phosphate buffered saline (PBS) or PBS liposomes (PBSLip) were injected as controls. The procedures to obtain ClLip and PBSLip were described elsewhere [66]. Blood from infected B6 mice was resuspended in 1 ml PBS, pipetted over 5 ml of 74% Percoll (GE Healthcare, USA) and centrifuged (2500 x g, acceleration/break 5/0) for 30 min at room temperature (RT). The top cell layers were collected and washed with complete RPMI 1640 medium (supplemented with 10% heat-inactivated fetal calf serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 50 µM 2-mercaptoethanol, 2 mM L-glutamine and 1 mM sodium pyruvate; Life Technologies, USA). Purified iRBCs (>95% purity) were stained with CTV or CMTPX, following the manufacturer’s instructions (Life Technologies). B6. CD11c-YFP mice infected with mCherry-Pc iRBCs were deeply anesthetized i. p. with 55 ng/g body weight of ketamine (Imalgene 1000, Merial, USA) and 0. 85 ng/g body weight of xylazine (Rompun 2%, Bayer, Germany). Spleens were externalized by a 1 cm incision just below the ribcage. Mice were placed above a metal plate with a coverslip and immobilized without disrupting the vasculature or splenic connective tissue. Live imaging was carried out with an Eclipse Ti microscope (Nikon Instruments Inc. , Japan) equipped with an Andor Revolution XD system (Andor Technology, UK), a Yokogawa CSU-X1 spinning disk unit (Andor Technology), a 20x PLAN APO VC objective (Nikon Instruments Inc.) and a 1. 5x auxiliary magnification system (Nikon Instruments Inc.). Data were processed with MicroManager 1. 2 (General Public License, NIH, USA). For each movie, 28 µm Z-sections with 4 µm Z-steps were acquired for 30 min. Imaris X64 7. 0. 0. (Andor Technology) was used to edit images and to determine the percentage of mCherry+YFP+ cells, as well as the CD11c+ cell volume and sphericity. In other cases, B6. CD11c-YFP mice were adoptively transferred with 5 × 106 splenic CD4+ T cells from B6. CFP mice (purified by FACS sorting using a FACSAria device; BD Biosciences). These mice were infected as described above and processed 24 h later. Imaris was used to edit images and to determine CD11c+ cell speed and displacement, as well as the coefficients of CFP+CD4+ T cell speed and arrest. B6. CD11c-YFP mice infected with CMTPX-Pc iRBCs were injected i. v. with PE-conjugated anti-F4/80 mAbs (200 ng/g body weight) and deeply anesthetized to externalize the spleen as described above. Live imaging was carried out with a Zeiss LSM 780-NLO confocal microscope (Zeiss, Germany). Data were processed with Zen 2012 software (Zeiss, Germany). In each movie, 28 µm Z-sections with 2 µm Z-steps were acquired for 30 min. Imaris was used to edit images and to determine the percentages of CMTPX+ cells. Mice were sacrificed and PBS-perfused to remove circulating iRBCs. Spleens were harvested, and the remaining RBCs were lysed with ACK lysis buffer. Splenocytes (1 × 106) were stained with fluorescent monoclonal antibodies (mAbs) against CD3, CD4, CD11c, CD69, CD11b, CD80, CD86, I-Ab, B220, CD36, CD64 (FcγRI), DX5 and Ter119 (BD Biosciences, USA), F4/80 (eBiosciences, USA), and MOMA-1 and MARCO (Abcam, UK). Cells were analyzed by flow cytometry (FACSCanto; BD Biosciences) with FlowJo 9. 5. 3. (Tree Star Inc. , USA). Splenocytes (3 × 107) were resuspended in 1 ml PBS with 0. 1% BSA (bovine serum albumin; Sigma-Aldrich) and stained with CFSE (carboxyfluorescein succinimidyl ester; Life Technologies) at a final concentration of 5 μM for 20 min at 37°C. Cells (1 × 106) were cultured in complete RPMI 1640 medium for 72 h at 37°C with 5% CO2 in the presence of iRBCs (3 × 106). Cells were then stained with fluorescent mAbs against CD3 and CD4, and proliferation was assessed by flow cytometry. IFN-γ was quantified in the supernatants using the OptEIA IFN-γ kit (BD Biosciences). GFP-Pc iRBC-infected B6 mice were sacrificed and PBS-perfused. Spleens were removed and frozen in Tissue-Tek OCT (Sakura Fineteck, Japan). Sections 8 µm thick were cut with a CM3050S Cryostat (Leica, USA) and fixed with 1% paraformaldehyde (Alfa Aesar, USA) for 30 min at RT. Sections were incubated with anti-CD16/CD32 mAb (Fc block; BD Biosciences) for 30 min followed by incubation in a humidified dark chamber with fluorescent mAbs against CD11c, CD19, CD3, CD4 (BD Biosciences) and MOMA-1 (Abcam) for 2 h at RT. Sections were then stained for 5 min with 0. 5 μg/ml DAPI (4' , 6-diamidino-2-phenylindole; Sigma-Aldrich), washed with PBS and mounted with Fluoromount-G (Southern Biotechnologies, USA). Images were acquired with a DMRA2 fluorescence microscope (Leica) and MetaMorph software (Molecular Devices Inc. , USA). Image analysis was performed with Photoshop CS4 (Adobe Inc. , USA). Percentages of CD11c-GFP/CD11c-CD4 pixel colocalization and of GFP pixel distribution in the spleen were calculated using FIJI for Windows 64-bit (Colocalization threshold and Mixture Modeling Thresholding plugins, respectively; General Public License, NIH, USA). Results were analyzed with Prism 5 software (Graph Pad) using ANOVA or Student’s t-tests. The existence of a normal distribution was confirmed using the Kolmogorov-Smirnov test. Differences were considered statistically significant at p < 0. 05.
Malaria is a significant health issue, particularly in the tropical and subtropical regions of the world. The red pulp (RP) of the spleen is a major site for the control of blood-borne infections such as malaria. Macrophages and dendritic cells (DCs) form a complex phagocyte network inside the splenic RP. DCs are usually thought of as highly efficient antigen-presenting cells that play an essential role in the activation of adaptive immunity. However, the direct role of DCs in the clearance of pathogens is still unclear. To clarify these issues, we took advantage of in vivo experimental approaches that enabled us to deplete or visualize DCs. The depletion of phagocytes demonstrated that DCs are key participants in the protection against blood stages of experimental malaria. Using confocal intravital microscopy, we observed that splenic RP DCs efficiently recognized and phagocytized infected erythrocytes during acute infection. We also showed that splenic DCs were crucial for the CD4+ T cell response to infection, but full DC maturation was achieved only after the peak of parasitemia. This study help to elucidate the protective mechanisms against Plasmodium parasites, and it shows that in vivo imaging is a reliable tool to evaluate iRBC phagocytosis during experimental malaria.
In Vivo Approaches Reveal a Key Role for DCs in CD4+ T Cell Activation and Parasite Clearance during the Acute Phase of Experimental Blood-Stage Malaria