Fatty acids affect a number of physiological processes, in addition to forming the building blocks of membranes and body fat stores. In this study, we uncover a role for the monounsaturated fatty acid oleate in the innate immune response of the nematode Caenorhabditis elegans. From an RNAi screen for regulators of innate immune defense genes, we identified the two stearoyl-coenzyme A desaturases that synthesize oleate in C. elegans. We show that the synthesis of oleate is necessary for the pathogen-mediated induction of immune defense genes. Accordingly, C. elegans deficient in oleate production are hypersusceptible to infection with diverse human pathogens, which can be rescued by the addition of exogenous oleate. However, oleate is not sufficient to drive protective immune activation. Together, these data add to the known health-promoting effects of monounsaturated fatty acids, and suggest an ancient link between nutrient stores, metabolism, and host susceptibility to bacterial infection.
Fatty acids are the key structural components of phospholipids and triglycerides, and thereby affect nearly every facet of eukaryotic physiology. In addition to forming the building blocks of membranes and functioning as a currency of energy storage, fatty acid molecules promote health in a diverse number of ways. For example, fatty acids act as soluble signals for intracellular communication, affect membrane fluidity, and have been directly linked to lifespan regulation [1–3]. Conversely, excess stores of fatty acids in triglycerides lead to atherosclerosis and type 2 diabetes [4]. Thus, it is important to understand how individual fatty acids affect key physiological processes within a cell. The nematode Caenorhabditis elegans is a valuable model for studying the roles of fatty acids in metazoan biology [5–10]. Through the sequential action of conserved elongase (elo) and desaturase (fat) genes, nematodes can synthesize the full range of fatty acid molecules found in plants and animals [5–9]. Thus, C. elegans does not have a dietary requirement for specific fatty acids, unlike mammals. In nematodes, as in mammals, the majority of fatty acid molecules are synthesized from stearic acid, a saturated, 18-carbon molecule, which is progressively desaturated and elongated to a variety of monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids (Fig 1A) [5–9]. The contribution of individual fatty acids to specific biological processes can be characterized using genetic approaches in C. elegans at the level of an entire organism. Nematodes rely on inducible host defense mechanisms to provide protection from ingested pathogens [11–14]. Because worms normally eat bacteria for food, their evolution has been shaped by interactions with both pathogenic and nonpathogenic microorganisms. The immune effectors in C. elegans include a suite of secreted proteins, including lysozymes, proteins with CUB-like domains, and ShK toxins, some of which are required for host defense during bacterial infection [15–18]. C. elegans with mutations that abrogate the induction of these immune effectors during infection are hypersusceptible to killing by bacterial pathogens [15,19,20]. In this study, we define a requirement for the MUFA oleate in innate immune activation and pathogen defense in C. elegans. Previously, Nandakumar et al. showed that two polyunsaturated fatty acids, γ-linolenic acid (GLA) and stearidonic acid (SDA), are required for the basal expression of innate immune effectors [17]. Here, we show that oleate is necessary for innate immune activation and resistance to bacterial infection in a manner distinct from the effects of GLA and SDA. Because oleate is among the most abundant fatty acids in cells, our data suggest an ancient link between cellular energy stores and immune activation.
We previously conducted an RNAi screen of 1,420 intestinal genes for innate immune regulators in C. elegans [21]. We used an immunostimulatory small molecule called R24 (also called RPW-24) and a GFP-based transcriptional reporter, irg-4: : GFP, which provides a convenient readout of innate immune activation [18,21–25]. The gene irg-4 (F08G5. 6) is transcriptionally upregulated during infection with multiple pathogens and contains a CUB-like domain, which is present in many of the secreted immune effectors in C. elegans [15,16,18]. irg-4 is required for normal resistance to bacterial infection, but does not modulate the normal lifespan of C. elegans or affect susceptibility to other stressors [16–18]. irg-4 is also strongly upregulated by R24, a xenobiotic that protects nematodes from bacterial infection by boosting innate immune responses [18,21,22,25]. Because of its potent immunostimulatory properties, R24 is a useful tool for dissecting the metabolic requirements of immune activation without altering the bacterial diet of C. elegans [18,21–25]. The RNAi screen identified 29 gene inactivations that are required for the R24-mediated induction of the innate immune reporter irg-4: : GFP [21]. Interestingly, two of the genes identified in this screen are the stearoyl-coenzyme A (CoA) desaturases fat-6 and fat-7, which function redundantly to synthesize oleate from stearic acid (Fig 1A) [5]. RNAi-mediated knockdown of fat-6 completely abrogated the induction of irg-4: : GFP by R24 (Fig 1B) while knockdown of fat-7 partially suppressed its upregulation (S1A Fig). To validate the results of the RNAi studies in the irg-4: : GFP transcriptional reporter, we used qRT-PCR to examine the transcriptional regulation of the irg-4 gene in wild-type and in fat-6 (tm331); fat-7 (wa36) double-mutant animals, which are deficient in oleate production [5,8]. The R24-mediated induction of irg-4 was reduced in fat-6 (tm331); fat-7 (wa36) animals compared to wild-type animals (Fig 1C). In addition, the induction of two other immune effectors, irg-5 (F35E12. 5) and irg-6 (C32H11. 1), was also attenuated in the fat-6 (tm331); fat-7 (wa36) double mutant (Fig 1C). Like irg-4, irg-5 and irg-6 are strongly induced during infection with the bacterial pathogen P. aeruginosa and by the immunostimulatory xenobiotic R24 [18,21–23]. In addition, knockdown of irg-5 and irg-6 makes C. elegans more susceptible to infection by P. aeruginosa [18]. The defect in immune activation by fat-6 (RNAi) was also visualized using the irg-5: : GFP transcriptional reporter (Fig 1D). Thus, stearoyl-CoA desaturases are required for the induction of at least three key immune effectors in C. elegans. We performed fatty acid supplementation experiments to determine if the effect of the stearoyl-CoA desaturases on immune activation depends specifically on the production of the MUFA oleate. Interestingly, supplementation of exogenous oleate rescued, in a dose-dependent manner, the R24-mediated immune activation defect of the irg-4: : GFP reporter strain in fat-6 (RNAi) animals (Fig 1B). Oleate is also required for the upregulation of irg-5: : GFP by R24, as supplementation of this MUFA rescued the induction defect conferred by knockdown of fat-6 (Fig 1D). Consistent with a key role for MUFAs in immune activation, knockdown of the elongase elo-2, which catalyzes the conversion of palmitic acid to stearic acid, the step immediately upstream of oleate synthesis, also suppressed the activation of irg-4: : GFP by R24 (Fig 1E). Importantly, oleate supplementation also fully complemented the immune activation defect of elo-2 (RNAi) animals (Fig 1E). These data demonstrate that lack of oleate, and not an accumulation of upstream stearic acid, is responsible for deficits in immune effector induction. In addition, knockdown of fat-5, the palmitoyl-CoA desaturase, which also preferentially acts on palmitic acid, but converts it to a different MUFA, palmitoleic acid (PLA), had no effect on the induction of irg-4: : GFP (Fig 1E). Our RNAi screen also identified the mediator subunit mdt-15 among the 29 gene inactivations that are required for the R24-mediated induction of the innate immune reporter irg-4: : GFP [21]. We subsequently showed that mdt-15 is required for the induction of innate immune effectors and for defense against the bacterial pathogen Pseudomonas aeruginosa [21]. In addition to its role as an immune regulator, MDT-15 controls the transcription of a suite of fatty acid biosynthesis enzymes [26–28]. Interestingly, oleate supplementation did not rescue the induction of irg-4: : GFP in mdt-15 (tm2182) loss-of-function animals, indicating that mdt-15 controls multiple steps in the activation of innate immune effectors (S1B Fig). Monounsaturated fatty acids are converted to polyunsaturated fatty acids (PUFAs) by desaturases that initially use oleate as a substrate [6]. To determine if a PUFA is required for immune effector induction by the immunostimulatory xenobiotic R24, we used both genetic and fatty acid complementation experiments. We examined the induction of irg-4: : GFP in animals deficient in the desaturase fat-2, which catalyzes the first step in PUFA synthesis (the conversion of oleate to linoleic acid), and also fat-1 (RNAi) and fat-3 (RNAi), the enzymes that act downstream of fat-2 in the synthesis of PUFAs (Fig 1A) [6,7]. Knockdown of fat-1, fat-2, or fat-3 had no effect on the induction of irg-4: : GFP by R24 (Fig 2A). We confirmed this RNAi experiment using the fat-2 (wa17) and the fat-3 (wa22) loss-of-function mutants (Fig 2B and 2C). Notably, the R24-mediated induction of the immune effectors irg-4, irg-5, and irg-6 in the fat-2 (wa17) and the fat-3 (wa22) mutants were not significantly lower than in wild-type animals (Fig 2B and 2C). Supplementation of individual fatty acids to fat-6 (RNAi) animals confirmed these genetic observations. Unlike oleate supplementation, addition of the 16 carbon MUFA palmitoleic acid (PLA), which is synthesized by the desaturase fat-5 (Fig 1A), did not complement the irg-4: : GFP induction defect in fat-6 (RNAi) animals (Fig 2D). In addition, supplementation of the PUFA linoleic acid (LA), which is synthesized by fat-2 using oleate as a substrate (Fig 1A), also failed to fully complement the irg-4: : GFP induction defect of fat-6 (RNAi) animals (Fig 2D). Together, these data show that the fatty acid oleate, and not another MUFA or PUFA, is required for the induction of the innate immune effector genes by an immunostimulatory small molecule. To determine if stearoyl-CoA desaturase activity has a broad effect on the induction of innate immune effectors, we profiled the transcription of 118 immune and stress response genes in wild-type, fat-6 (RNAi), and fat-3 (RNAi) animals, each exposed to the solvent control (DMSO) or R24 (Fig 3A). Of the 40 genes that were induced at least 4-fold by R24, the upregulation of 16 genes was significantly attenuated in fat-6 (RNAi) animals (Fig 3A and 3B and S1 Table). As we observed in our studies of irg-4, irg-5, and irg-6 in the fat-3 (wa22) mutant (Fig 2C), the induction of these 16 fat-6-dependent genes was not affected by knockdown of fat-3 (Fig 3A and 3B). For this transcription profiling experiment, we chose to use fat-6 (RNAi) to examine the effects of oleate depletion on immune activation. Others have also used single knockdown of either fat-6 or fat-7 to recapitulate the phenotypes observed in the fat-6 (tm331); fat-7 (wa36) double mutant [3,29]. Of note, the R24-mediated upregulation of irg-4, irg-5, and irg-6 was not attenuated in the fat-6 (tm331) or fat-7 (wa36) single mutants (S2A and S2B Fig), but the induction of these immune effectors was suppressed in fat-6 (RNAi) animals (Fig 3B), as in the fat-6 (tm331); fat-7 (wa36) double mutants (Fig 1C). We also confirmed by gas chromatography-mass spectrometry (GC-MS) that knockdown of fat-6 significantly decreases the pool of oleate and causes accumulation of the upstream fatty acid stearate (S1C Fig). Interestingly, each of the 16 fat-6-dependent, fat-3-independent genes encode putative immune effectors that are induced during infection with at least one bacterial pathogen, a group that includes the innate immune effectors irg-4, irg-5, and irg-6 (Fig 3B). Twenty-four genes, however, were induced by R24 in a manner independent of fat-6 (S1 Table). Thus, fat-6 modulates the transcription of a specific subset of genes, including a group of innate immune effectors. Moreover, the observation that the induction of these 16 genes was not controlled by fat-3 further supports the specificity of fat-6 in the regulation of innate immune responses. Of the 16 genes whose R24-mediated induction was dependent on fat-6, ten are putative immune effectors that are also induced during infection with P. aeruginosa, including three known modulators of the host susceptibility to pseudomonal infection, irg-4, irg-5, and irg-6 [16–18]. To determine if oleate is important for host defense in C. elegans, we performed pathogenesis assays with P. aeruginosa. The fat-6 (tm331); fat-7 (wa36) double mutant was more susceptible to infection by P. aeruginosa than wild-type animals, consistent with a prior report [17] (Fig 4A and S2A Table). Importantly, fat-6 (tm331); fat-7 (wa36) animals have a similar lifespan as wild-type animals when grown under standard laboratory conditions [30]. These data suggest that the hypersusceptibility to pathogen-mediated killing in the fat-6 (tm331); fat-7 (wa36) double mutant is not secondary to pleiotropic effects of these mutations on worm fitness. Supplementation of oleate to the fat-6 (tm331); fat-7 (wa36) animals fully complemented the enhanced susceptibility of this mutant to pathogen infection (Fig 4A and S2A Table). Of note, fat-6 (tm331) and fat-7 (wa36) single mutant worms are not more susceptible to pathogen-mediated killing than wild-type animals, as noted previously [17] (S3 Fig and S2B Table). Consistent with the key role of fat-6 and fat-7 in the regulation of innate immune responses, the fold induction of the innate immune effectors irg-4, irg-5, irg-6, irg-1, and irg-2 during pseudomonal infection was significantly attenuated in the fat-6 (tm331); fat-7 (wa36) double mutant compared to wild-type (Fig 4B). Nandakumar et al. previously defined a role for two PUFAs, GLA and SDA, in the basal regulation of innate immune effectors and pathogen resistance in C. elegans [17]. We considered whether the effect of oleate on the activation of immune responses could occur through its metabolism to GLA and SDA; however, several lines of evidence show that this is not the case. The pathogen susceptibility and immune effector transcription profile of fat-2 (wa17) mutants demonstrate that the effect of oleate on innate immune activation is not dependent on the production of PUFAs. The desaturase fat-2 acts immediately downstream of oleate production to catalyze the first step in PUFA biosynthesis (Fig 1A). fat-2 (wa17) mutant animals are not more susceptible to P. aeruginosa infection than wild-type animals (Fig 4C and S2A Table). In addition, the induction of the immune effectors irg-4, irg-5, irg-6, irg-1, and irg-2 during pseudomonal infection was not compromised in fat-2 (wa17) mutants compared to wild-type (Fig 4D), unlike what we observed for the fat-6 (tm331); fat-7 (wa36) double mutant (Fig 4B). We also examined the desaturase fat-3, which acts downstream of fat-2 in the synthesis of PUFAs, including GLA and SDA (Fig 1A). Nandakumar et al. previously showed that fat-3 is required for resistance to P. aeruginosa via the fatty acids GLA and SDA [17]. We found that exogenous oleate did not rescue the enhanced susceptibility of the fat-3 (wa22) mutant to pseudomonal infection (S4 Fig and S2D Table). Thus, fat-6 and fat-7 affect pathogen susceptibility specifically through the production of oleate, in a manner that is independent of PUFA synthesis via the enzymes fat-2 or fat-3. Stearoyl-CoA desaturases are required for the induction of immune effectors, such as irg-4, irg-5, and irg-6, whose basal, or resting, expression is dependent on the p38 MAPK PMK-1 innate immune pathway and those, like irg-1 and irg-2, whose transcription are regulated independent of this canonical immune pathway (Fig 4B) [15,18,21,31]. Interestingly, knockdown of tir-1, the Toll/IL-1 (TIR) domain protein that is an integral component the p38 MAPK PMK-1 signaling cassette [19,32,33], further enhanced the susceptibility of the fat-6 (tm331); fat-7 (wa36) double loss-of-function mutant strain to pseudomonal infection (Fig 4E and S2A Table). Likewise, knockdown of the bZIP transcription factor zip-2, which controls the induction of irg-1 and irg-2 during P. aeruginosa infection [31], caused the fat-6 (tm331); fat-7 (wa36) double mutant to be more susceptible to killing by P. aeruginosa (Fig 4F and S2A Table). These data suggest that fat-6 and fat-7 are required for the proper expression of a broad group of innate immune effectors via a mechanism that operates in parallel to the p38 MAPK PMK-1 and ZIP-2 immune pathways. We performed GC-MS to determine if R24 treatment changes the abundance of cellular oleate. Interestingly, GC-MS revealed that the fraction of both oleate and linoleic acid relative to the total fatty acid pool significantly increased in R24-treated samples compared to controls (Fig 5A). Together, these data show that treatment with the immunostimulatory xenobiotic R24 shifts the fatty acid pool towards more oleate. Because oleate is required for the induction of innate immune effectors and is increased in the presence of R24, we asked if this MUFA is sufficient for innate immune activation in C. elegans. However, the addition of oleate to the standard bacterial food source for C. elegans did not activate GFP expression in the irg-4: : GFP or the irg-5: : GFP transcriptional reporters (Fig 5B and 5C). The presence of oleate in the growth media also did not further augment the induction of irg-5: : GFP during P. aeruginosa infection (Fig 5C). In addition, oleate treatment did not extend the lifespan of wild-type C. elegans during P. aeruginosa infection (Fig 5D and S2C Table). Thus, oleate is necessary, but not sufficient, for immune activation and resistance to P. aeruginosa infection in C. elegans. Interestingly, fat-6 (tm331); fat-7 (wa36) mutant animals were also hypersusceptible to infection with the gram-positive pathogen Enterococcus faecalis (Fig 5E and S2C Table) and Serratia marcescens (Fig 5F and S2C Table), which, like P. aeruginosa, is a gram-negative bacteria. Importantly, the enhanced susceptibility of the fat-6 (tm331); fat-7 (wa36) mutants to infection with E. faecalis and S. marcescens was rescued by treatment with exogenous oleate (Fig 5E and 5F). Thus, oleate is required for host resistance to diverse bacterial pathogens.
This study defines a role for the MUFA oleate in C. elegans innate immune activation. We show that animals deficient in oleate production were hypersusceptible to killing by the bacterial pathogens P. aeruginosa, E. faecalis, and S. marcescens in a manner dependent on oleate. Oleate is among the most abundant fatty acids in cells. Thus, these data may explain how a metazoan animal limits the induction of protective immune defenses to times when the host has accumulated sufficient energy reserves to survive challenge from bacterial pathogens. Nandakumar et al. previously identified two fatty acids, GLA and SDA, which are synthesized by the enzyme fat-3 and are required for the basal expression of immune effectors [17]. Our data indicate that oleate and these PUFAs affect immune effector expression and pathogen resistance by different mechanisms. C. elegans with a loss-of-function mutation in fat-2, the enzyme that catalyzes the first step in PUFA synthesis, induced innate immune effector genes normally and were not more susceptible to P. aeruginosa pathogenesis. It is also important to note that knockdown of fat-3 did not affect the R24-mediated induction of 16 fat-6-dependent innate immune effectors. In addition, exogenous supplementation of PUFAs to fat-6 (RNAi) animals did not restore immune effector expression, whereas the addition of oleate fully complemented the induction defect of these animals. Also of note, the effect of fat-3 (wa22) on susceptibility to bacterial infection was independent of oleate. Han et al. recently found that oleate is sufficient to extend the lifespan of nematodes that were grown under standard laboratory conditions [1]. Interestingly, we found that treatment with oleate is not sufficient to provide protection during bacterial infection, but is required for proper immune gene transcription. Specifically, oleate is important for the pathogen-mediated induction of immune effectors that are downstream of the p38 MAPK PMK-1 pathway and for genes that are regulated by the bZIP transcription factor ZIP-2, which functions independently of the canonical PMK-1 pathway to mediate an early transcriptional response to P. aeruginosa infection [31]. Consistent with these data, RNAi mediated knockdown of tir-1, a component of the p38 MAPK PMK-1 signaling cassette, as well as zip-2, enhanced the susceptibility of fat-6 (tm331); fat-7 (wa36) animals to P. aeruginosa infection. Thus, oleate has diverse, health-promoting effects on lifespan and pathogen resistance in C. elegans. In plants, oleate is also required for the proper expression of immune defense genes and resistance to pathogen infection, suggesting that the role for oleate in immune activation may be strongly conserved [34,35]. However, the mechanism by which oleate regulates immune defenses is not known in either C. elegans or plants. Our supplementation studies indicate that oleate treatment itself does not activate immune gene transcription. Thus, oleate is unlikely to be a signal of immune activation in C. elegans, but rather functions as a licensing factor for the elaboration of anti-pathogen responses. Disruption of oleate biosynthesis alters membrane fluidity, which has pleiotropic consequences on membrane-bound organelles, including activating stress pathways associated with endoplasmic reticulum dysfunction [3]. Indeed, alterations of membrane fluidity have been linked to activation of G protein-coupled receptors [36,37]. Thus, changing the oleate content in C. elegans may modulate the ability of the host to mount protective defense responses, either directly or by disrupting lipid-protein interactions that are essential for immune pathway activation. Our findings present a previously unappreciated link between a highly abundant fatty acid and immune activation, which may represent an ancient connection between body energy stores and susceptibility to bacterial infection.
C. elegans strains were maintained on E. coli OP50 or HT115 bacteria on nematode growth media plates, as described [38]. The C. elegans strains used in this study were N2 Bristol [38], AU306 agIs43 [irg-4: : GFP: : unc-54-3’UTR; myo-2: : mCherry] [21], AY101 acIs101 [pDB09. 1 (irg-5: : GFP); pRF4 (rol-6 (su1006) ) ] [39], BX156 fat-6 (tm331); fat-7 (wa36) [9], BX106 fat-6 (tm331) [5], BX153 fat-7 (wa36) [5], BX30 fat-3 (wa22) [6], and BX26 fat-2 (wa17) [6]. P. aeruginosa strain PA14 [3], E. faecalis strain MMH594 [40], and S. marcescens strain Db11 [41] were used in this study. Fatty acids were obtained from Nu-Chek-Prep, Inc. and were prepared as previously described [42]. Assays were performed by growing synchronized L1 worms on the indicated fatty acid or control media containing tergitol (0. 1%). Unless otherwise indicated, oleate was used at a concentration of 400 or 500 μM, except for Fig 1E, which used 1 mM. Palmitoleic acid and linoleic acid were used at a concentration of 500 μM. “Slow killing” P. aeruginosa pathogenesis assays were performed as previously described [43]. The E. faecalis [40,44] and S. marcescens [45] pathogenesis assays were performed as previously described. For the assays with fatty acid supplementation, L4 stage-matched C. elegans, raised from the L1 to the L4 stage on E. coli HT115 on media containing fatty acids or control, were transferred to standard assay plates for the indicated experiment, which were not supplemented with fatty acids. Sample sizes, mean lifespan, and p values for all trials are shown in S2 Table. The protocol for treatment of animals with 70 μM R24 has also been described [22]. The RNAi screen of 1,420 RNAi clones was previously described [21]. RNAi clones that were used in this study are from the Ahringer [46] or Vidal [47] libraries and were confirmed by sequencing. The codeset used for the NanoString nCounter Gene Expression Analysis was synthesized by NanoString and contained probes for 118 C. elegans genes, which has been described previously [21,23]. Counts from each gene were normalized to three control genes: snb-1, ama-1, and act-1. The qRT-PCR studies were performed as described previously [21–23], using previously published primer sequences [8,15,21–23]. All values were normalized against the control gene snb-1. Fold change was calculated using the Pfaffl method [48]. Synchronized populations of approximately 6,000 worms at the L4 stage were harvested 24 hours after exposure to 70 μM R24 or 1% DMSO control, washed with M9 buffer to remove excess bacteria, and frozen in ethanol on dry ice. Worm pellets were thawed, sonicated, and then dissolved in 1 mL of a 3: 1 methanol: methylene chloride mixture with 50 μl of internal standard dissolved in hexane (17: 0, Nu-Chek-Prep Inc.). While vortexing, 200 μl acetyl chloride was slowly added. Samples were subjected to methanolysis at 80°C for 1 hour. After cooling to room temperature, the sample was neutralized with 4 mL of 7% K2CO3, and fatty acid methyl esters were extracted through the addition of 2 mL of hexane. Following hexane addition, samples were vortexed and then centrifuged at 2,500 rpm for 10 minutes. The top hexane layer containing fatty acid methyl esters was transferred to a new borosilicate glass test tube and washed with 2 mL acetonitrile, vortexed, and centrifuged at 2,500 rpm for 5 min. The top hexane layer was transferred and dried under nitrogen. Fatty acid methyl esters were resuspended in 200 μl hexane, vortexed, and transferred to Agilent vials with glass insert. Fatty acid methyl esters were analyzed by GC-MS using an Agilent 6890/5972 GC-MS system outfitted with a Supelcowax 10 column as previously described [49,50]. The relative abundance of each fatty acid was determined by dividing each fatty acid by the total fatty acid pool. Nematodes were paralyzed with 10 mM levamisole (Sigma), mounted on agar pads and photographed using a Zeiss AXIO Imager Z2 microscope with a Zeiss Axiocam 506mono camera and Zen 2. 3 (Zeiss) software. C. elegans survival was assessed using the Kaplan-Meier method and differences were determined with the log-rank test using OASIS 2 [51]. Other statistical tests, which are indicated in the figure legends, were performed using Prism 7 (GraphPad Software).
The evolution of multicellular organisms has been shaped by their interactions with pathogenic microorganisms. The microscopic nematode C. elegans eats bacteria for food and has evolved inducible immune defenses toward ingested pathogens that are coordinated within intestinal epithelial cells. C. elegans, therefore, presents a genetic system to characterize the requirements for the activation of innate immune defenses. Here, we show that the monounsaturated fatty acid oleate is necessary for the induction of innate immune defenses and for protection against bacterial pathogens, which defines a new link between metabolism and the regulation of anti-pathogen responses in a metazoan host.
invertebrates
medicine and health sciences
rna interference
pathology and laboratory medicine
caenorhabditis
immune activation
pathogens
immunology
microbiology
animals
pseudomonas aeruginosa
animal models
oleates
caenorhabditis elegans
model organisms
experimental organism systems
epigenetics
bacteria
bacterial pathogens
research and analysis methods
pseudomonas
lipids
animal studies
genetic interference
medical microbiology
gene expression
microbial pathogens
immune response
biochemistry
rna
eukaryota
nucleic acids
immunity
genetics
nematoda
biology and life sciences
fatty acids
organisms
The fatty acid oleate is required for innate immune activation and pathogen defense in Caenorhabditis elegans