I am trying to learn recombinant DNA technology. The experiment involves designing 2 oligonucleotides composed of a 5 bp 5' tail of arbitrary sequence, a restriction site polylinker, and the annealing region to amplify a template sequence. I then need to document my experiment in this format:
"PCR ca1067F/ca1067R on pSB1AK3-b0015 (1054bp, pcrpdt)
Digest pcrpdt (EcoRI/SpeI, 10+1038+6, 1, pcrdig)
Digest pSB1A2-I13521 (EcoRI/SpeI, 2062+946, 0, vectdig)
Ligate pcrdig and vectdig (pSB1A2-Bca9128)
-----------------------------------------
>ca1067F Forward Biobricking of KanR of pSB1AK3 
ccagtGAATTCgtccTCTAGAgagctgatccttcaactc
>ca1067R Reverse Biobricking of KanR of pSB1AK3 
gcagtACTAGTtccgtcaagtcagcgtaatg"
I am then supposed to predict the sequence of the PCR product, the various digestion products, and the final ligation product. Do you understand what this means?
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