Yes.
The Secret Room is a special room type. One secret room will appear on every floor in the game and will have a pre-determined location when the floor is spawned.

The Secret room cannot be entered normally; ways to enter include:

XXVIII The Moon Tarot Card
Random Teleport
Bombing in through any adjacent room (excluding boss rooms and mini-boss rooms)
Using the X-Ray Glasses 


Ways to identify the Secret room on the map include:

The Treasure Map
The Spelunker Helmet (when in an adjacent room)
XXI The World
XIX The Sun
X-Ray Glasses (when in an adjacent room)
The Compass (will be identified as a mini-boss room if and ONLY if Greed is in the Secret Room) 


The secret room is typically placed adjacent to three or four other adjacent rooms, often adjacent to a Treasure Room if possible. If applicable, the Secret Room will always have preference over three adjacent rooms to two rooms. This preferrence does not occur when comparing three adjacent rooms to four adjacent rooms. 

Does every floor contain a secret room?
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