Extracellular matrix (ECM) fluoresces, especially Collagen and Laminin. The maximum is in the DAPI and FITC channels and the fluorescence becomes weaker towards longer wavelengths. However, since the coat on the TC flasks is very thin, I would not expect this to be a problem. The best thing is just to try it. There is also a quite famous document available which might be of help:

Autofluorescence: Causes and Cures

How do different tissue culture matrices affect background in fluorescent microscopy?
I have a Hifiberry DAC for a model B and would like to use it on b+ or pi 2. I know the GPIO pins have changed from model B layout - would it be possible to figure out the old and new layout differences and wire the DAC to the new pi this way? 

The P5 header doesn't seem to exist anymore, although it seems the first 26pins remain the same. As far as a gather the P5 header was used to transfer the audio data from the board to the DAC. So if I can find which pins replace these would it be a simple case of rewiring these pins or is there a fundamental difference here I am missing?
