Using histological stains on expectorated samples from phlegm (also called "sputum"), scientists can identify MTB under a microscope. Since MTB retains certain stains even after being treated with acidic solution, it is classified as an acid-fast bacillus. The most common acid-fast staining techniques are the Ziehl–Neelsen stain and the Kinyoun stain, which dye acid-fast bacilli a bright red that stands out against a blue background. Auramine-rhodamine staining and fluorescence microscopy are also used. COUNT 5
QSN1 What's another word for "sputum"? ANS1 phlegm QSN2 One of the two standard acid-fast staining techniques is the Kinyoun stain; what's the other? ANS2 Ziehl–Neelsen stain QSN3 What color will acid-fast bacilli become when stained? ANS3 bright red QSN4 What's the term for bacillus that can be exposed to acidic solutions without losing their stains? ANS4 acid-fast QSN5 If a scientist didn't want to use an acid-fast staining technique, what type of microscopy could they use instead? ANS5 fluorescence