The system consists of stabilized microtubule, kinesin motors (constructed with light-induced hetero-dimer system) and an energy mix. All ingredients and buffer preparation protocol are documented in a previous paper by Ross et. al.[1]}, and we follow the exact same procedure in our study. The sample chambers are made by sandwiching pre-cut Parafilm M by coated slides and coverslips [1]}, [3]}. The measured depth of the chamber is approximately 80\(\mu \) m.
