As stain variation is the dominant challenge when analysing histology images from various scanners, in the first step of our proposed pipeline we used Vahadane et al.'s method [1]} to normalise the stain intensities of all images in the training set to the target image 009, with the help of TIAtoolboxhttps://github.com/TIA-Lab/tiatoolbox library. Note the same stain matrix acquired from image 009 is used on-the-fly during the prediction on test images.
