To summarize, a crucial modeling challenge is that multiple factors ranging from cell differentiation to chemotactic cues are involved in vasculogenesis in vivo, and modeling approaches based on different hypotheses can show network pattern formation [1]}. A physical model that captures the formation of such EC networks purely through mechanical interactions can then help to isolate the different factors that might drive cell clustering into networks. Such modeling will help efforts to use mechanical cues to guide the self-assembly of ECs into organized networks as a prelude to vasculogenesis, a key requirement for the engineering of viable tissue [2]}. This will guide experimental strategies to tune substrate mechanical properties to generate vascular networks with morphology optimal for biological transport functions.
