The microorganisms are composed of the following seven types according to the different application
domains, including agricultural microorganisms, environmental microorganisms, food microorganisms, industrial microorganisms, medical microorganisms, water-borne microorganisms and other microorganisms.
Then, the digital images are obtained after staining and slicing by using professional equipment [1]}.
The following part is pre-processing, including image denoising and contrast enhancement, which is helpful for image segmentation.
The next step is biovolume measurement methods [2]}.
Biovolume measurement aims to measure the morphological parameters and calculate the biovolume.
The most significant part of microorganism biovolume measurement is image segmentation.
Finally, the evaluation methods are proposed.
However, the results obtained in these studies are area and volume, that are difficult to be compared with the
results obtained by manual methods.
So the proper evaluation methods can help to analyze the performance of the image analysis methods [3]}.
