The above mentioned stoichiometric models are conceived to perform Flux Balance Analysis (FBA): a technique that exploits linear programming to compute the flux through each reaction under a steady state assumption [1]}. The advantage of FBA is its use without knowledge about the enzymatic kinetic constants. FBA is based on the maximization (or minimization) of a given objective function. To simulate growth of tumor cells, maximization of biomass production is typically assumed. To perform a correct FBA it is necessary to define the nutritional constrains, i.e., the incoming nutrients and the outcoming products (exchange reactions). Unluckily this kind of data are yet rare in public databases.
