"Stanford University scientists have described a new type of test that can detect genetic mutations in minute amounts of DNA released from cancer cells into the blood. The test which is called single color digital PCR requires only a fraction of a tube of blood and can detect as few as three mutationbearing molecules in a single reaction. According to the report httpdx.doi.org.j.jmoldx... in The Journal of Molecular Diagnostics httpjmd.amjpathol.org this highly sensitive test has the potential to be personalized to recognize mutations unique to any individual cancer. For monitoring patient tumors only a handful of blood tests are available which are limited to only several types of cancers. Nearly all cancer patients require monitoring by whole body imaging which can be costly complex and timeconsuming. In contrast molecular tests like the one we have developed will enable patients to be monitored at every visit and thus have the potential for quickly tracking cancer growth and spread. Moreover the tests rapid turnaround and relatively low cost especially compared to nextgeneration DNA sequencing provide a potential opportunity for universal monitoring of more patients than is currently done explained lead investigator Hanlee P. Ji MD Associate Professor in the Department of Medicine at Stanford University and Senior Associate Director of the Stanford Genome Technology Center. The report describes the use of the test to analyze samples from six patients. Five patients were previously diagnosed with colorectal cancer and one with cholangiocarcinoma.  After generation of customized mutation detection assays the researchers were able to identify tumorderived circulating DNA from three out of six patients. In one patient the assay was able to show the presence of three different mutations. The three patients whose samples did not show elevated cancer DNA were undergoing active treatment at the time of collection. The singlecolor digital PCR test offers several advantages over other methods of circulating tumor DNA analysis compared to nextgeneration targeted sequencing and fluorescent probebased digital PCR assays. The main advantage is that the new technique does not rely on preamplification which can introduce errors and biases.  This test is simple enough to set up and analyze without extensive training and therefore it can be implemented by anyone making it highly accessible to any laboratory. It has been truly motivating to work with a technology that will help transform the way that we monitor and treat individuals with cancer. I am excited to share our findings with the cancer research community noted lead author and researcher Christina Wood Bouwens of the Stanford Genome Technology Center and the Division of Oncology Department of Medicine Stanford University School of Medicine Stanford California."