"Nearly every American who has become a parent in the last decade has heard the slogan breast milk is best and has likely been encouraged to offer breast milk to newborns. Among other things breast milk contains natural sources of prebiotics small indigestible fiber molecules that promote the growth of good bacteria in the babys gut. Yet for many families breastfeeding is difficult or impossible. Fortunately modern infant formulas are getting closer to the real thing with the help of University of Illinois researchers.  In a recent study from the Piglet Nutrition and Cognition Lab at U of I scientists worked with piglets to show prebiotics included in infant formula can enhance memory and exploratory behavior.  When we provide prebiotics in formula our results confirm that we can not only benefit gut health which is known but we can also influence brain development says Ryan Dilger associate professor in the Department of Animal Sciences Division of Nutritional Sciences and Neuroscience Program at U of I. We can actually change the way piglets learn and remember by influencing bacteria in the colon. Piglets are widely considered a more informative model for human infants than mice and rats their digestive systems behavioral responses and brain development are remarkably similar to human infants. Therefore researchers are increasingly turning to piglets to test hypotheses in preclinical trials related to human health especially in the context of gut microbes and brain development.  There hasnt been a lot of work looking at the gutbrain axis in humans but a lot of rodent work is showing those connections. This is taking it to an animal model that is a lot closer to human infants and asking if that connection still exists and if we can tease out possible mechanisms says Stephen Fleming lead author of the study and a doctoral student in the Neuroscience Program at U of I. In early  Dilger and his colleagues worked with piglets to show that a combination of innovative formula components including prebiotics may play a role in brain development and behavior. In their new study the team concentrated solely on the effects of prebiotics. Starting on the second day of life piglets were given a cows milkbased infant formula supplemented with polydextrose PDX a synthetic carbohydrate with prebiotic activity and galactooligosaccharide GOS a naturally occurring prebiotic. When the piglets were  days old Fleming took them through several learning memory and stress tests. After  days blood brain and intestinal tissues were collected for analysis. The test for learning and memory gave piglets a chance to play with dog toys one theyd seen before and one brandnew toy. If they spent more time with the new toy that was an indication that the piglet recognized it as new and preferred it. This novel object recognition test improves on classic maze tests commonly used in rodent studies.  If youre trying to test for memory this test is closer to what wed do with an infant. After all we dont generally train infants on mazes Fleming says. We know from previous research this test works for pigs but this is the first published example of using it in a nutrition context. Pigs fed PDX and GOS spent more time playing with new objects than pigs who didnt receive the prebiotic supplements. The preference for novel objects an indication of natural curiosity is a sign of healthy brain development and points towards positive development of learning and memory. When prebiotics are working the way they should good bacteria increase in abundance. One way to tell is by looking at metabolic endproducts  volatile fatty acids VFAs  excreted by bacteria during digestion of prebiotic fibers.  Volatile fatty acids are a global indicator for whether prebiotics had an effect on the overall population of bacteria. For example we might want to see an increase in Lactobacillus and other beneficial bacteria that produce butyrate Dilger explains. Volatile fatty acid VFA concentrations in the colon blood and brain were changed in pigs receiving PDX and GOS compared with control pigs.  Recent evidence suggests that bacterial VFAs could be getting into the blood and traveling to the brain where they could potentially affect mood and behavior.  We found that yes VFAs are absorbed in the blood of pigs that were fed PDXGOS. And yes they do get into the brain Fleming explains. But when we looked at the relationship between these VFAs and the results of our behavior tests there did not appear to be a clear connection.  Another surprise was a decrease in serotonin in brains of pigs fed the prebiotic. When you hear less serotonin theres an immediate reaction to say Well thats bad Fleming says. Not necessarily those pigs didnt show greater anxiety than control pigs during a stress test or poorer performance when given a learning and memory test. The researchers hypothesize that the prebiotics may alter levels of tryptophan serotonins amino acid precursor but its too early to say. Although more work is needed to tackle remaining questions the study adds to the growing body of research suggesting a strong and potentially modifiable link between the gut and the brain a link that makers of infant formula should strongly consider. There are so many ways we can alter the composition of the microbiota and they can have very strong benefits. Promoting good gut health remains a strong focus in the field of nutrition Dilger says.  The article Dietary polydextrose and galactooligosaccharide increase exploratory behavior improve recognition memory and alter neurochemistry in the young pig is published in Nutritional Neuroscience. Fleming and Dilgers coauthors include Supida Monaikul Alexander Patsavas Rosaline Waworuntu and Brian Berg. The work was supported by Mead Johnson Nutrition."