Phase And Vertical Shift. Phase and vertical shift refer to the transfer of a function in the horizontal and vertical directions, respectively. For the function , where A, B, C, and D are constants, the phase shift is defined as C/B. If the phase shift is positive, the shift occurs to the left; if the phase shift is negative, the shift occurs to the right.
So it is possible that a whole food meal will have a different impact on the duration of protein synthesis than pure amino acids. Our lab has recently shown that the duration of protein synthesis in response to a complete meal containing protein, carbohydrates, and fats is approximately 3 hours long. Therefore, it appears that a complete meal slightly prolongs the duration of protein synthesis.
The samples are then heated at 900Cfor 3 minutes and then loaded onto the gel, which is not pre-run priorto sample loading. Protein gels are run more slowly than nucleic acid gels,and consequently may require more time. A gel 20 cm in length generallyruns for about 3-4 hours.The time required is of course variable dependingon the nature of the sample. The samples are run into the stacking gelat about 2-3 watts for 30 minutes or so, after which the power is increasedto 8-10 watts until completion.REPARING A PROTEIN GEL: Threebasic reagents are required that can be prepared from the list of materialsabove: (1) acrylamide gel buffers, (2) electrophoresis buffer, and (3)sample loading buffer.