Ciliate conjugation is a sexual phenomenon that results in genetic recombination and nuclear reorganization within the cell. In most ciliate groups, however, the cells separate after conjugation, and both form new macronuclei from their micronuclei.
Before the bun, hamburgers are said to have been served between two pieces of bread. In fact, a ground beef patty was known as Hamburger steak (first mentioned in an American cookbook in 1891); when this was put between bread or in a bun it was called a Hamburger sandwich.
According to Congresswoman Rosa DeLauro, the hamburger, a ground meat patty between two slices of bread, was first created in America in 1900 by Louis Lassen, a Danish immigrant, owner of Louis' Lunch in New Haven, Connecticut.