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Differential Staining. Gram stain technique is a differential staining technique, which separates bacteria into two groups (discussed in earlier chapters), Gram-positive bacteria and Gram-negative bacteria. Another differential staining technique is acid-fast technique which differentiates species of Mycobacterium from other bacteria.
2. Differential staining for cell wall characteristics is also used as part of a classification scheme, so this particular method is used for both identification and classification.(See Table 10.5). 3. The presence of various enzymes, as determined by biochemical tests, is used in identifying microorganisms.dentification Methods. 1. Morphological characteristics are useful in identifying microorganisms, especially when aided by differential staining techniques (Gram staining and acid fast stains can suggest a course of treatment before the organism is positively identified).