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Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.Tryptone is commonly used in microbiology to produce lysogeny broth for the growth of E. coli and other microorganisms. It provides a source of amino acids for the growing bacteria.ryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.
Answers. Best Answer: Luria Broth (or more correctly Lysogeny Broth, LB), consists of tryptone, yeast extract, and salt (NaCl). Tryptone is a digest of casein (cheese proteins), and it provides the peptides that the bacteria need.Yeast Extract consists of broken down baker's yeast.lthough LB broth and agar are very popular media to grow bacteria on, there are no sugars in LB. The only carbon sources for the bacteria are the amino acids (protein building blocks).