Salt-out the enzyme with a saturated ammonium sulfate solution as in Step 1. Record the volume of the saturated salt solution added. Collect the protein precipitates. The precipitates may be collected by following a similar washing procedure as in Step 1. Try not to dilute the enzyme solution too much.n a typical protein preparation or purification step carried out in a laboratory where the aim is to isolate a small quantity of a product for structural or kinetic studies, a saturated ammonium sulfate solution is routinely used. It is also the procedure taken in this experiment.
A phenotype (from Greek phainein, meaning to show, and typos, meaning type) is the composite of an organism 's observable characteristics or traits, such as its morphology, development, biochemical or physiological properties, phenology, behavior, and products of behavior (such as a bird's nest). phenotype results from the expression of an organism's genes as well as the influence of environmental factors and the interactions between the two. When two or more clearly different phenotypes exist in the same population of a species, the species is called polymorph.
The phenotype is the total characteristics displayed by an organism under a particular set of environmental factors, regardless of the actual genotype of the organism. It results from the expression of the genes of an organisms as well as the influence of environmental factors and random variation.