Fanatical Mad Scientist. Akihiro Kanou (åç´ æå, KanÅ Akihiro), often referred to as Professor Kanou (åç´ææ, KanÅ-kyÅju), is a physician at Kanou General Hospital and is the central character responsible for turning Ken Kaneki into an Artificial One-Eyed Ghoul..
Hardy Diagnostics Pseudomonas Isolation Agar is a modification of the formula developed by King, et al. (1) The medium is designed to enhance pyocyanin production, thereby improving the differentiation of pseudomonads.
However, the lack of an easy and fast protocol for the isolation and culture of these cells leads to long incubating times with the use of high number of rat brains. Here, we describe a quick protocol based on mixed glial culture for the highly-enriched isolation of microglia, astrocyte and oligodendrocyte cells.