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Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.Tryptone is commonly used in microbiology to produce lysogeny broth for the growth of E. coli and other microorganisms. It provides a source of amino acids for the growing bacteria.ryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.
Bacteria are identified based largely on what organic compounds they can break down. The range of compounds used depends on the collection of enzymes a species of bacteria can make. Tryptophan is an amino acid that some bacteria can use for carbon and energy.Tryptone broth is rich in tryptophan, and thus serves as an excellent medium to use for testing for tryptophan digestion.acteria are identified based largely on what organic compounds they can break down. The range of compounds used depends on the collection of enzymes a species of bacteria can make. Tryptophan is an amino acid that some bacteria can use for carbon and energy.