Ciliate conjugation is a sexual phenomenon that results in genetic recombination and nuclear reorganization within the cell. In most ciliate groups, however, the cells separate after conjugation, and both form new macronuclei from their micronuclei.
Before the bun, hamburgers are said to have been served between two pieces of bread. In fact, a ground beef patty was known as Hamburger steak (first mentioned in an American cookbook in 1891); when this was put between bread or in a bun it was called a Hamburger sandwich.
Each of these first burgers was first served between bread or (in New Haven) toast, he notes. Whoever it was who first plunked a hamburger patty on a bun and undertook to sell this product is the true inventor of the American hamburger, but probably destined to forever remain unknown.