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In general, whenever the diagnosis of meningitis is strongly considered, a lumbar puncture should be promptly performed. Examination of the cerebrospinal fluid (CSF) is the cornerstone of the diagnosis. The diagnosis of bacterial meningitis is made by culture of the CSF sample.
Two bacterial broth cultures, showing a positive result (left side) and a negative result (right side) for the VP test. VogesâProskauer /ËfoÊÉ¡És ËprÉskaÊ.Ér/ or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result. The test depends on the digestion of glucose to acetylmethylcarbinol. If glucose is being broken down, it will react with alpha-naphthol (VP reagent #1) and potassium hydroxide (VP reagent #2) to form a red color. Alpha-naphthol and potassium hydroxide are chemicals that detect acetoin.