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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Making the world better, one answer at a time. In the third stage, anaphase, the sister chromatids of. each replicated chromosome begin to separate. Fibers pull the centromere apart and chromatids move away from each other, toward opposite ends of the. cell.