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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
When cell replication is necessary, a cell enters the cell cycle. Prior to the mitosis phase of the cycle, the cell undergoes a period of growth where it replicates its DNA and organelles. Prophase. In the first stage of mitosis called prophase, the replicated chromatin fibers form chromosomes. Each replicated chromosome consists of two chromatids (sister chromatids) connected at the centromere region. Chromosome centromeres serve as a place of attachment for spindle fibers during cell division.