Typical costs: 1  Costs for a groom's cake are similar to those for wedding cakes, ranging from $1-$2 or less per slice for a relatively straightforward single-tier or sheet cake, to $8-$10 or more a slice for elaborate cakes with fondant, gum paste and sugar decorations or fruit.
Immunofluorescence microscopy is used to localize specific constituents in tissue sections or immobilized cells using fluorescent tags as labels.
From this step on there are two commonly used detection systems, immunofluorescence or immunoperoxidase. For the immunofluorescence method, slides are next incubated with 10% normal goat serum for 37oC for 45 min to block nonspecific binding. This is followed by incubation with an appropriate dilution of adduct specific antibody in blocking solution for 45 min at 37oC or at 4oC overnight.