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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
During the telophase stage, the chromosomes uncoil and revert to their extended form, which is manifested in the homogeneous appearance of chromatin displayed by interphase chromosomes. Also in late telophase, the chromosomal fibers disappear and nucleoli develop at the nucleolar organizing sites. Nuclear envelopes start to form around the nuclei from remnants of the parent nuclear envelope and portions of the endomembrane system contained in each of the daughter cells. In addition, the cytoplasmic network of microtubules begins to once again define the cellular structure. The nuclear membrane is reassembled during late telophase from vesicles that surround individual chromosomes, which eventually fuse into larger groups that finally associate to complete the structure. During this process, nuclear pores are established and the lamins, which have been dephosphorylated during anaphase, condense to reform the nuclear lamina.