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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
The main difference between interphase and the first stage of mitosis is that the chromatin is diffuse and unorganized, according to Nature Education. Prophase, the first stage of cell division, occurs when the chromatin coils and condenses to form the chromosomes. During this stage, the chromosomes are visible under a microscope showing the two chromatids (strands) connected by a centromere.