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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Animal cell cytokinesis[edit] Animal cell cytokinesis begins shortly after the onset of sister chromatid separation in the anaphase of mitosis. A contractile ring, made of non-muscle myosin II and actin filaments, assembles equatorially (in the middle of the cell) at the cell cortex (adjacent to the cell membrane).