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In general, whenever the diagnosis of meningitis is strongly considered, a lumbar puncture should be promptly performed. Examination of the cerebrospinal fluid (CSF) is the cornerstone of the diagnosis. The diagnosis of bacterial meningitis is made by culture of the CSF sample.
Cerebrospinal fluid (CSF) analysis. This is a primary diagnostic tool for encephalitis and meningitis. CSF analysis is a group of common tests, and a wide variety of other tests, that can be ordered and performed on a sample of CSF. It is collected using a procedure called a lumbar puncture or spinal tap. Initial CSF tests âThe initial basic set of CSF tests that are often performed with suspected infections of the central nervous system include: 1  Physical characteristics: normal CSF appears clear and colorless.