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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Quick Answer. In the anaphase II stage of meiosis, the sister chromatids are separated and begin moving to opposite poles of the cell, according to About.com. When the paired sister chromatids are fully separate, then each is considered to be a full chromosome. These are known as daughter chromosomes.