To prepare 1 L of either 1Ã or 10Ã PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
Cardiovascular disease is a term that refers to more than one disease of the circulatory system including the heart and blood vessels, whether the blood vessels are affecting the lungs, the brain, kidneys or other parts of the body. Cardiovascular diseases are the leading cause of death in adult Canadian men and women.
The heart, blood, and blood vessels make up the cardiovascular component of the circulatory system. It includes the pulmonary circulation, a loop through the lungs where blood is oxygenated.iew full size image. One of the most common diseases of the circulatory system is arteriosclerosis, in which the fatty deposits in the arteries causes the walls to stiffen and thicken the walls. According to the Mayo Clinic, the causes are a buildup of fat, cholesterol and other material in the artery walls.