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The Entangled Bank. Definition: Spindle fibers are aggregates of microtubules that move chromosomes during cell division. During prophase of mitosis, spindle fibers form at opposite poles of the cell. During metaphase, spindle fibers extend from the cell poles toward the midpoint of the cell known as the metaphase plate.Chromosomes are held at the metaphase plate by the equal forces of the spindle fibers pushing on the centromeres of the chromosomes.In anaphase, spindle fibers pull sister chromatids toward the spindle poles. Spindle fibers not connected to chromatids lengthen and elongate the cell.uring metaphase, spindle fibers extend from the cell poles toward the midpoint of the cell known as the metaphase plate. Chromosomes are held at the metaphase plate by the equal forces of the spindle fibers pushing on the centromeres of the chromosomes.
Spindle fibers are necessary in mitosis as they help in bringing half the number of chromosomes on each side of the cell, so that when the cell divides, the chromosomes are distributed equally in both the daughter nuclei.uring mitosis and meiosis the spindle fibers allow the chromosomes  to line up along the equator of the cell. The chromosomes slide to  either pole during cell division. 1 person found this useful.