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The Entangled Bank. Definition: Spindle fibers are aggregates of microtubules that move chromosomes during cell division. During prophase of mitosis, spindle fibers form at opposite poles of the cell. During metaphase, spindle fibers extend from the cell poles toward the midpoint of the cell known as the metaphase plate.Chromosomes are held at the metaphase plate by the equal forces of the spindle fibers pushing on the centromeres of the chromosomes.In anaphase, spindle fibers pull sister chromatids toward the spindle poles. Spindle fibers not connected to chromatids lengthen and elongate the cell.uring metaphase, spindle fibers extend from the cell poles toward the midpoint of the cell known as the metaphase plate. Chromosomes are held at the metaphase plate by the equal forces of the spindle fibers pushing on the centromeres of the chromosomes.
The function of the spindle fiber is to guide the sister chromatids  to opposite poles. The spindle fibers are what the chromosomes  attach to.uring mitosis and meiosis the spindle fibers allow the chromosomes  to line up along the equator of the cell. The chromosomes slide to  either pole during cell division. 1 person found this useful.