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The Entangled Bank. Definition: Spindle fibers are aggregates of microtubules that move chromosomes during cell division. During prophase of mitosis, spindle fibers form at opposite poles of the cell. During metaphase, spindle fibers extend from the cell poles toward the midpoint of the cell known as the metaphase plate.Chromosomes are held at the metaphase plate by the equal forces of the spindle fibers pushing on the centromeres of the chromosomes.In anaphase, spindle fibers pull sister chromatids toward the spindle poles. Spindle fibers not connected to chromatids lengthen and elongate the cell.uring metaphase, spindle fibers extend from the cell poles toward the midpoint of the cell known as the metaphase plate. Chromosomes are held at the metaphase plate by the equal forces of the spindle fibers pushing on the centromeres of the chromosomes.
Spindle Fibers. spindle fibers A collection of microtubules attached to a centromere during mitosis and meiosis that are responsible for the movement of the chromosomes to opposite poles. Spindle fibers align the chromosomes along the middle of the cell nucleus. This line is referred to as the metaphase plate. The protein fibers formed during prophase of nuclear division; chromosomes attach to these fibers at the centromere. spindle The aggregation of microtubules that is involved in the movement and separation of chromosomes during mitosis and meiosis.