Define frustration: the act of frustrating; the state or an instance of being frustrated â frustration in a sentence the act of frustrating; the state or an instance of being frustratedâ¦ See the full definition
The definition of a blot is a spot or stain, whether physical or on a person's character. An example of a blot is an ink stain on a shirt. Blot means to dry, soak up or remove. An example of to blot is to wipe up tipped over milk with a paper towel.
Blots are created by laying a membrane over one face of the gel and then creating a flow which carries the molecules in the gel onto the membrane. The two most common methods used for Northern and Southern blotting are capillary flow and vacuum transfer. Electroblotting is also used occasionally, although it requires special care to prevent crushing or melting of the agarose gel. Capillary flow transfers are carried out in a dish of buffer (see figure below).