Blood type (or blood group) is determined, in part, by the ABO blood group antigens present on red blood cells. A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs).These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system. blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs).
Received July 13, 1990. DNA polymerase from Thermus aquaticus has be-. come a common reagent in molecular biology because of. its utility in DNA amplification and DNA sequencing. protocols. A simplified method is described here for iso-. lating the recombinant Taq enzyme after overproduc-. tion in Escherichia coli. Purification requires 8 to 10 h. and entails heat treating and clearing the E. coli lysate, followed by precipitation of the enzyme with polyethy-.
Therefore it replaced the DNA polymerase from E. coli originally used in PCR [7]. Taq's temperature optimum for activity is 75-80 0 C, with a half-life of 9 minutes at 97.5 0 C, and can replicate a 10 3 base pair strand of DNA in less than 10 seconds at 72 0 C [8].The incorporation of dNTPs into DNA is catalyzed by Taq DNA polymerase I.aq DNA polymerase has among the highest error rates of the thermophilic polymerases at 285 x 10 -6 errors per template nucleotide. Tli polymerase has a proof reading ability that is five-fold better than Taq at 57 x 10 -6 errors per template nucleotide and Pfu polymerase also demonstrates fidelity in this range [10].