Blood type (or blood group) is determined, in part, by the ABO blood group antigens present on red blood cells. A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs).These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system. blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs).
Received July 13, 1990. DNA polymerase from Thermus aquaticus has be-. come a common reagent in molecular biology because of. its utility in DNA amplification and DNA sequencing. protocols. A simplified method is described here for iso-. lating the recombinant Taq enzyme after overproduc-. tion in Escherichia coli. Purification requires 8 to 10 h. and entails heat treating and clearing the E. coli lysate, followed by precipitation of the enzyme with polyethy-.
Unit Assay Conditions. 1X Poly(A) Polymerase Reaction Buffer, 1 mM rATP and 500 ng 5Â´ FAM labeled poly A 20-mer RNA in a 20 Âµl reaction. After incubation at 37Â°C for 10 minutes the length of the poly(A) addition is determined either by gel electrophoresis or with an automated capillary DNA sequencer.