To prepare 1 L of either 1Ã or 10Ã PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
Cardiovascular disease is a term that refers to more than one disease of the circulatory system including the heart and blood vessels, whether the blood vessels are affecting the lungs, the brain, kidneys or other parts of the body. Cardiovascular diseases are the leading cause of death in adult Canadian men and women.
Circulatory system problems arise when there is limited blood flow to the legs, hands, heart and rest of the body, including the fingers, toes and feet. The blood vessels become blocked as a fatty substance called plaque builds up and hardens and constricts the walls of the arteries and veins.