Blood type (or blood group) is determined, in part, by the ABO blood group antigens present on red blood cells. A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs).These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system. blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs).
Received July 13, 1990. DNA polymerase from Thermus aquaticus has be-. come a common reagent in molecular biology because of. its utility in DNA amplification and DNA sequencing. protocols. A simplified method is described here for iso-. lating the recombinant Taq enzyme after overproduc-. tion in Escherichia coli. Purification requires 8 to 10 h. and entails heat treating and clearing the E. coli lysate, followed by precipitation of the enzyme with polyethy-.
DNA Polymerase III of Escherichia coli PURIFICATION AND IDENTIFICATION OF SUBUNITS* (Received for publication, July IO, 1978) DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83.he denaturing second dimension. DNA Polymerase III of E. coli 1751 1st dimension polymerase III 7 r impurity I B - --a ---\--, = , impurities / ,130,000 - 94,000 - 30,000 I IL 0.93 0.63 0.17 Rf I FIG. 3. A, two-dimensional electrophoresis of DNA polymerase III.