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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
In the staining process, those that decolorize easily are gram negative, and the one that retain the primary stain are gram positive.   The acid fast staining is a techique that stains only the gram positive and only the genuses of mycobacterium, nocardia, and actinomycetes.ram staining   Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped â¦ as Gram-positive, and those that do not retain the color are grouped as Gram-negative.