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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
Spore staining depends on increasing the permeability of the spore coat by heating to permit the dye in. â¦ Upon cooling the dye is trapped inside the spore and not allowed out.The primary dye malachite green is a relatively weakly binding dye to the cell wall and spore wall.In fact, if washed well with water, the dye comes right out of the cell wall, however not from the spore wall once the dye is locked in. That is why there does not need to be a decolorizer in this stain.ithout heat fixing it when you stain the slide and then wash it off to see the results that bacteria sample will wash it off with it. Also the heat fixes the bacteria in plac â¦ e. 4 people found this useful. Deb Answers.