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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
Best Answer: Gram stain stains specific molecules in the cell wall of bacteria. Gram positive bacteria stain purple, gram negative stain pink. Since human cells lack a cell wall there is nothing to retain the purple stain. When the counter-stain, safranin is applied, the cells will be light pink. Source(s): Biology instructor for 39 years.