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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
This is provided as a reference. By the end of the course you will be able to recognize most of these microorganisms. Gram stain and bacterial morphology: Of all the different classification systems, the Gram stain has withstood the test of time.Discovered by H.C. Gram in 1884 it remains an important and useful technique to this day.ome bacteria such as mycobacteria (the cause of tuberculosis) are not reliably stained due to the large lipid content of the peptidoglycan. Alternative staining techniques (Kinyoun or acid fast stain) are therefore used that take advantage of the resistance to destaining after lengthier initial staining.