It is for this reason that is referred to as a complementary DNA or cDNA. Though there are numerous processes for synthesizing cDNA, the best way of doing so is by using mature or fully spliced mRNA. This is usually done by using the enzyme reverse transcriptase.
Ammonia is used as refrigerant prominently in the refrigeration systems of food industry like dairies, ice creams plants, frozen food production plants, cold storage warehouses, processors of fish, poultry and meat and number of other applications.
The rapid evaporation causes the temperature of the liquid to drop until it reaches the normal boiling point of -28Â°F, a similar effect occurs when water evaporates off the skin, thus cooling it. This is why ammonia is used in refrigeration systems. Liquid anhydrous ammonia weighs less than water.