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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
In a simple stain a basic dye is added to a population of bacteria that has been fixed to the surface of a microscope slide such that all cells in the population take on the color of the dye used. In the second set of exercises you will be performing a. Gram stain. (a type of.