It is for this reason that is referred to as a complementary DNA or cDNA. Though there are numerous processes for synthesizing cDNA, the best way of doing so is by using mature or fully spliced mRNA. This is usually done by using the enzyme reverse transcriptase.
Ammonia is used as refrigerant prominently in the refrigeration systems of food industry like dairies, ice creams plants, frozen food production plants, cold storage warehouses, processors of fish, poultry and meat and number of other applications.
An ammonia refrigeration system, like all vapor-compression refrigeration systems, are made up of a number of components working together. Essentially, the ammonia is the chemical that is contained within the system to remove heat from an area, and then dissipate it in another area.