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Within eukaryotes, DNA replication is controlled within the context of the cell cycle. As the cell grows and divides, it progresses through stages in the cell cycle; DNA replication takes place during the S phase (synthesis phase).
Quick Answer. DNA replication occurs within the nucleus of a cell. During the cell cycle S phase, any time that a cell needs to divide, DNA replication occurs. Prior to cell division, the DNA replication process ensures the copying of chromosomes. Helicases and SSB proteins partially unwind the double helix strand.