T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75â80 Â°C, with a half-life of greater than 2 hours at 92.5 Â°C, 40 minutes at 95 Â°C and 9 minutes at 97.5 Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 Â°C.
Glucose and fructose are monosaccharides and are the building blocks of sucrose, a disaccharide. Thus, disaccharides are just a pair of linked sugar molecules. They are formed when two monosaccharides are joined together and a molecule of water is removed -- a dehydration reaction.
Disaccharides are a simple-sugar form of carbohydrate made up of two linked monosaccharides, of which there are three: glucose, fructose, and galactose. Examples include table sugar or sucrose, which is glucose linked to fructose, and milk sugar or lactose, which is glucose linked to galactose.Disaccharides may also be formed by two of the same monosaccharides bonding to each other. Maltose, for instance, is a pair of glucose molecules.Sources include sugar beets and sugar cane, which produce sucrose, a sugar found in everything from baked goods to pasta sauce. They can be found in milk, yogurt, and other dairy products, which contain lactose, and grains and beer, which contain maltose.Also known as a biose, a disaccharide is a quick-digesting form of carbohydrate that the body uses for immediate energy.altose, for instance, is a pair of glucose molecules. Sources include sugar beets and sugar cane, which produce sucrose, a sugar found in everything from baked goods to pasta sauce. They can be found in milk, yogurt, and other dairy products, which contain lactose, and grains and beer, which contain maltose.