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A look at different types of techniques used to separate mixtures: magnetism, decant, filtration, distillation, chromatography.
Salts and other small molecules are retarded by the desalting column and are thereby separated from the target molecules. Desalting is sometimes called group separation mode. In fractionation by size, all the molecules to be separated are below the size exclusion limit of the column, and are retained by the column.