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Within eukaryotes, DNA replication is controlled within the context of the cell cycle. As the cell grows and divides, it progresses through stages in the cell cycle; DNA replication takes place during the S phase (synthesis phase).
Replication starts at the beginning of phase S and ends during S phase (usually at the end of S phase). The above means that sister chromatids are NOT present in all phase S. Sister chromatinds are present at a small part of phase S (when the replication of the DNA is finished.).