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Within eukaryotes, DNA replication is controlled within the context of the cell cycle. As the cell grows and divides, it progresses through stages in the cell cycle; DNA replication takes place during the S phase (synthesis phase).
DNA occurs during the S phase of interphase, sandwiched between the G1 and G2 phases. The cell uses checkpoint signals to ensure at the end of G1 that it is big enough to replicate and at the end of G2 to determine whether or not DNA replication has succeeded.