Mandela, the former president of the Republic of South Africa and Nobel Peace laureate, spent more than 40 yearsâ27 of them in prisonâas a central figure in the struggle against South Africaâs brutal and restrictive racial regime called apartheid.
meristem culture. (shoot-tip culture) The culture of excised meristems on suitable nutrient media under aseptic conditions. The stem apex is usually used though axillary meristems may also be taken.Often gibberellic acid must be added to the medium to promote normal growth into a plantlet.shoot-tip culture) The culture of excised meristems on suitable nutrient media under aseptic conditions. The stem apex is usually used though axillary meristems may also be taken. Often gibberellic acid must be added to the medium to promote normal growth into a plantlet.
We excise the meristem under the microscope (0.3 mm long) and culture it on tissue culture media. The resulting plant from the meristem is grown in the greenhouse and tested for viruses. Virus-free plants are tested by DNA fingerprinting methods to assure that the correct variety is to be returned to the customers. brief description of the steps involved is as follows: Step 1: Meristem culture Several meristems are cultured to obtain several plants for later testing. Generally the success rate is 20% in virus elimination. Step 2: Virus testing All 17 viruses known to be present in California are tested by PCR method.