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Plymouth was colder, whereas Jamestown was warmer and more humid, and therefore more prone to harbor diseases. I'm sure that there were a lot more differences, but those are the big ones that leapt into my mind.
Jamestown was a settlement in the Colony of Virginia, and the first permanent English settlement in the Americas. It was established by the Virginia Company of London as 'James Fort' on May 14, 1607 after several failed earlier attempts, and served as the capital of the colony for 83 years, from 1616 until 1699.