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Plymouth was colder, whereas Jamestown was warmer and more humid, and therefore more prone to harbor diseases. I'm sure that there were a lot more differences, but those are the big ones that leapt into my mind.
Jamestown (or James Towne or Jamestowne) was a settlement located on Jamestown Island in the Virginia Colony. Founded as James Fort on May 14, 1607, it was the first permanent English settlement in what is now the United States of America, following several earlier failed attempts, including the Lost Colony of Roanoke. It was founded by the London Company (later to become the Virginia Company), headquartered in England.