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Using neutral red pH indicator, the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-)fermentation. This medium is also known as an indicator medium and a low selective medium. Presence of bile salts inhibits swarming by Proteus species.
Lactic acid, also known as 2-hydroxypropanoic or milk acid, is a compound formed when glucose is broken down under certain conditions in a living creature or by some types of bacteria. In a person, for example, it is an important part of producing energy for strenuous exercise and helps with certain liver functions.