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Anaphylactic reactions are excluded by the presence of red plasma or urine, discordant pretransfusion and posttransfusion ABO blood types, or a positive direct antiglobulin (Coombs) test result. Demonstration of anti-IgA in a pretransfusion sample of the recipient's serum or plasma establishes the diagnosis.
A urine specimen must be collected in a clean and dry container. A first morning urine. specimen is preferred since it generally contains the highest concentration of hCG; however, urine specimens collected at any time of the day may be used.