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Test results indicate that salt has a significant effect on the low temperature performance of asphalt binders; Freezeâthaw is a main influencing factor when the percentage of salt is less than 3%, and the erosion effect of salt accelerates the failure of asphalt mixtures when the percentage of salt is more than 3%.
Introduction. The thawing procedure is stressful to frozen cells, and using good technique and working quickly ensures that a high proportion of the cells survive the procedure. As with other cell culture procedures, we recommend that you closely follow the instructions provided with your cells and other reagents for best results.