Spinach is the quintessential healthy food, and itâs no surprise that itâs also high in potassium. Bananas get a lot of attention for their potassium content, but ounce for ounce spinach bests them in the potassium department.
New Applications. Quantitative polymerase chain reaction (qPCR) is used to simultaneously detect a specific DNA sequence in a sample and determine the actual copy number of this sequence relative to a standard. In real-time PCR, the DNA copy number can be established after each cycle of amplification.
Determining the exact number of mRNA or DNA copies using PCR or qPCR method is called absolute quantification. This is rather a very simple method were you generate a standard curve with a known quantity of your gene of interest and determine the copies of the target in an unknown sample.