DNA purity (A 260 /A 280) = (A 260 reading â A 320 reading) Ã· (A 280 reading â A 320 reading). Strong absorbance around 230nm can indicate that organic compounds or chaotropic salts are present in the purified DNA. A ratio of 260nm to 230nm can help evaluate the level of salt carryover in the purified DNA.otal yield is obtained by multiplying the DNA concentration by the final total purified sample volume. DNA yield (Âµg) = DNA concentration Ã total sample volume (ml). However, DNA is not the only molecule that can absorb UV light at 260nm.
Automatism: An unconscious movement that may resemble simple repetitive tics or may be a complex sequence of natural-looking movements. This curious type of behavior occurs in a number of neurological and psychiatric disorders. The neurologic disorders associated with automatisms include narcolepsy (the sudden recurrent uncontrollable compulsion to sleep) and some forms of epilepsy such as especially psychomotor epilepsy. The psychiatric conditions associated with automatisms include schizophrenia (catatonic type) and fugue (flight) states. Automatisms involve doing something automatically and not remembering afterwards how one did it or even that one did it. Automatisms are also called automatic behavior.
(ÉËtÉmÉtÉn ) Word forms: plural automatons , plural automata (ÉËtÉmÉtÉ ) 1. countable noun. If you say that someone is an automaton, you are critical of them because they behave as if they are so tired or bored that they do things without thinking. [disapproval] I get sick of being thought of as a political automaton.