Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
Rafael LeÃ³nidas Trujillo Molina (Spanish pronunciation: [rafaËel leËoniÃ°as tÉ¾uËxiÊo]; 24 October 1891 â 30 May 1961), nicknamed El Jefe (Spanish: [el Ëxefe], The Chief or The Boss), was a Dominican politician and soldier, who ruled the Dominican Republic from February 1930 until his assassination in May 1961.