In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
A renowned baritone was forced to pull out of a performance of Cosi fan tutte on Saturday after falling ill â leaving the director to take his place while another performer sang the role from the side of the stage. Sir Thomas Allen in Cosi Fan Tutti at the Royal Opera House Photo: ALASTAIR MUIR.