In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
And because of that, they stopped performing the song live in 1987. Tutti Frutti Little Richard admits it's about anal sex. I packaged this list as songs ruined by artist revelations; this is a case where the revelation makes the song SO much better. You know the lyrics of Tutti Frutti as just random sounds strung together, almost Adam Sandler style. A whop bop-a-lu-mop, a whop bam boo.