In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
#18 Nessun Dorma (Concert, 1948) 1  Mario Lanza sang the thrilling aria Nessun Dorma from Giacomo Puccini's 12th and final opera, Turandot (composed 1921-1924; first performed 1926), in concert at the Hollywood Bowl on July 24, 1948. The composer MiklÃ³s RÃ³zsa conducted the Hollywood Bowl Orchestra.