In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
A significant portion of success of Tutti Frutti is attributed to the so-called âballet Cin Cinâ (in the original Italian, âragazze cin cinâ). This was a group of internationally well-known models, who were hired permanently to fill certain roles in the show.