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Nutrient Agar: composition, preparation and uses 4.58/5 (19) Nutrient agar is used as a general purpose medium for the growth of a wide variety of non-fastidious microorganisms. It consists of peptone, beef extract and agar. This relatively simple formulation provides the nutrients necessary for the replication of a large number of non-fastidious microorganisms.
In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms 1. Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required.escription Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.