In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
In the night club, Ron plays jazz flute in the style of Ian Anderson, lead singer and flautist of Jethro Tull. Ron blurts out Hey Aqualung! at the end of the song, a lyric from the Tull song Aqualung, the title track of their 1971 album. In addition, the riff that he plays on the flute just before he does so is the main riff of the same song.