In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
(July 2009) (Learn how and when to remove this template message) Tutti Frutti was the German version of the Italian game show Colpo Grosso (Big Score). It was aired from 21 January 1990 through to 21 February 1993 on RTL plus for three seasons, totaling approximately 140 episodes.