In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Puccini's famous aria is something of a pop hit. After Pavarotti's version stormed the pop charts in 1990, the song has taken on a life of its own. Karen Cummingsâs soprano voice soars above contemporary grooves in an ambient pop version of Nessun Dorma on Australian DJ-composer Daz Nuanceâs DiVAR!A Arias (2005).