Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
Alfonso XIII of Spain. Alfonso XIII (Spanish: Alfonso LeÃ³n Fernando MarÃ­a Jaime Isidro Pascual Antonio de BorbÃ³n y Habsburgo-Lorena; 17 May 1886 â 28 February 1941) was King of Spain from 1886 until the proclamation of the Second Republic in 1931. Alfonso was monarch from birth as his father, Alfonso XII, had died the previous year.