Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
Government in Spain. Spain's current government is relatatively new, having been organized from scratch following the 1975 death of Spanish dictator Francisco Franco after a 35-year reign. The Spanish constitution, which bestowed upon Spain a long-awaited democratic Parliamentary Monarchy, was drafted and decided upon in 1978.