In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
July 20, 2007 â¢ Turandot, Puccini's extravagant portrayal of ancient China, is home to Nessun dorma! â one of music's most famous numbers â a soaring tenor aria that's been recorded by performers ranging from Franco Corelli and Placido Domingo, to the Twelve Girls Band and the Scottish Fiddle Orchestra.