In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
In 2007, an eclectic panel of renowned recording artists voted Tutti Frutti No. 1 on Mojo's The Top 100 Records That Changed The World, hailing the recording as the sound of the birth of rock and roll.. The song is #43 in Rolling Stone's list of The 500 Greatest Songs of All Time.