In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
But Blackwell hired a second songwriter to re-write the words to the song. The band did three takes in the studio, and voilÃ : Little Richard's first hit single was made. In September 1955, Tutti Frutti climbed to the top of the R&B charts and hit #17 on Billboard's pop chart. It was followed by sixteen more Little Richard hits in the next three years. Almost overnight, Little Richard had made it big.