In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
The only recording to follow Puccini's score exactly was the very first, sung by Gina Cigna and Francesco Merli, conducted by Franco Ghione. In Alfano's completion of act 3, the Nessun dorma theme makes a final triumphal appearance at the end of the opera. The theme also makes a concluding reappearance in Luciano Berio 's later completion (this having been an expressed intention of Puccini's), but in a more subdued orchestration.