In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Kim Wilson is an American blues singer and harmonica player. He is best known as the lead vocalist and frontman for the Fabulous Thunderbirds on two hit songs of the 1980s, Tuff Enuff and Wrap It Up.. Wilson wrote Tuff Enuff, the group's only Top 40 hit.