Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
Charles Quint (or Carlos I) ruler of Spain, the Netherlands, Austria, Germany and America - the most powerful man in Europe. King Henry VIII of England (1509-1547) - France sought him as an ally at the Field of the Cloth of Gold, but he turned to Spain.