In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Come tutti possono vedere, i seggi sono tutti vuoti, fatta eccezione per una bella signora in grigio che Ã¨ un funzionario e che non puÃ² prendere la parola. Everybody can see that the seats are empty except for one charming lady in grey who is an official and is not allowed to speak.