In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Though commercially successful singles like Bill Haley & The Cometsâ âRock Around The Clockâ (1954), Little Richardâs âTutti Fruttiâ (1955) and Elvis Presleyâs âHound Dogâ (1956) were among the songs that popularized the genre and made it a household word, they didnât invent it.