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Nutrient Agar: composition, preparation and uses 4.58/5 (19) Nutrient agar is used as a general purpose medium for the growth of a wide variety of non-fastidious microorganisms. It consists of peptone, beef extract and agar. This relatively simple formulation provides the nutrients necessary for the replication of a large number of non-fastidious microorganisms.
Best Answer: Peptone is used in media as food for bacteria. It is obtained through partial hydrolysis of a protein by an acid or enzyme. Agar is an unbranched polysaccharide made from the cell walls of red algae or sea weed.It is used as a microbiological culture medium by dissolving it in hot water, and then cooling it to form a jelly.Agar and peptone are used together for bacterial cultures.t is used as a microbiological culture medium by dissolving it in hot water, and then cooling it to form a jelly. Agar and peptone are used together for bacterial cultures.