Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
Juan Carlos I of Spain. Juan Carlos I (born 5 January 1938) reigned as King of Spain from 1975 to 2014. His name came from his father (Juan de BorbÃ³n), his grandfather (Alfonso XIII) and his mother's father's name (Prince Carlo of the Two Sicilies). On 22 November 1975, two days after the death of Francisco Franco, Juan Carlos became king.