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Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.
The melting temperature, T m, of an oligonucleotide is its most critically important value. The most reliable and accurate determination of melting temperature is determined empirically, however, this is cumbersome and not usually necessary.he melting temperature for the sequence TGCTCA is, 2(1+2) + 4(1+2) = 18Â°C. The nature of the immobilized target strand provides a net decrease in the T m observed when both target and probe are free in solution. The magnitude of the decrease is approximately 7-8Â°C.