The exact causes of chronic kidney failure are unknown, although the debilitating loss of healthy kidney tissue may be a natural endpoint for several readily recognizable conditions, including advanced dental disease and a variety of kidney infections, inflammations and obstructions.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
1 Get the DNA extraction kit at the Daly Ralston Resource Center. 2  This kit is very popular-make sure to reserve it. 3  If you don't have the kit available: - Prepare the lysis buffer, and fill class set of labeled tubes with 12ml of the solution and seal.NA extraction is a fairly simple procedure that requires a few steps: 1  The lysis buffer (detergent) breaks open the cells by destroying the fatty membranes that enclose the cells as well as the nuclei membranes within the cells. 2  DNA is released into the solution.