Preparation of MacConkey Agar. 1  Suspend the measured amount of powder (See in the agar bottle and generally 50 gram) in 1 L of purified water and mix thoroughly. 2  Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3  Autoclave at 121Â°C for 15 minutes.
All USDA loans (purchase and refinance) require a one-time Guarantee Fee that is added to the borrowers loan. The current amount of the USDA guarantee fee is 2%. Example â if a borrower was purchasing a home 100% financing for $150,000, the adjusted loan amount with the 2% USDA guarantee fee would be $153,000. $3,000 added to the base loan amount. In addition to this one time Guarantee Fee, USDA loans also require a annual fee that is added to the monthly mortgage payment. Basically the âannual feeâ is monthly mortgage insurance â or more commonly known as âPMIâ Again, like the above it can have many different names. The current amount of this fee is .5%.
Appraised value â $126,000. In this example it would be ok to roll in all of your closing costs into the new loan, the home appraisal supports it. Your total loan amount would be $125,000 plus the 2% USDA guarantee fee. Now you have your USDA contract fully approved and closing costs are taken care of as well.What else can the home buyer expect to pay? Generally you will be required to pay an escrow or earnest money deposit when you write your purchase contract. This amount is usually around $500- $1,000 depending on the sales price.our total loan amount would be $125,000 plus the 2% USDA guarantee fee. Now you have your USDA contract fully approved and closing costs are taken care of as well.