The exact causes of chronic kidney failure are unknown, although the debilitating loss of healthy kidney tissue may be a natural endpoint for several readily recognizable conditions, including advanced dental disease and a variety of kidney infections, inflammations and obstructions.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
Nalidixic Acid is a chemotherapy drug which is effective against gram negative bacteria. It works by inhibiting DNA replication through enzyme inhibition. It is often used to treat urinary tract infections. gram negative bacteria is a bacteria which does not retain the violet stain during the gram staining procedure. It is different from a gram positive bacteria, which does retain the violet stain.