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The Ohio River ends at the confluence with the Mississippi River at Cairo, Illinois. This confluence borders Kentucky, Illinois and Missouri. Even though the Ohio River is much wider and carries more water than the Mississippi where they meet, the Ohio is considered the tributary to the Mississippi.
This boundary became referred to as the Mason-Dixon line because it began in the east along the Mason-Dixon line and headed westward to the Ohio River and along the Ohio to its mouth at the Mississippi River and then west along 36 degrees 30 minutes North.