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Meatloaf is done when a meat thermometer inserted in its center reads 160 degrees Fahrenheit. Ground beef used for food items like hamburgers or meatloaf should be at this temperature after it is cooked.
The internal temperature of the meatloaf should register 170 F for beef or 185 F for pork. To help this happen, let meatloaf stand about 20 minutes or longer after removal from the oven before serving. During this time the meatloaf will cook internally.