Our MSK physicians specialize in bone and joint imaging, spinal imaging, metabolic bone disease, and osteoporosis. Imaging techniques include plain films, CT, Ultrasound, and MRI. For diagnostic purposes, the division also performs plain films, arthrography, bone biopsies, and peripheral soft tissue lesions.
If you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.If you want genomic DNA, you crack your cells open in a different way and try to isolate as much of the stuff as possible.f you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.
Each colony contains a different plasmid type, because each was grown up from a single transformed cell. What we do now is to isolate DNA from each colony (or a small growth of cells propagated from the colony), and analyze the structure of the plasmid with restriction enzymes or by DNA sequencing.