Our MSK physicians specialize in bone and joint imaging, spinal imaging, metabolic bone disease, and osteoporosis. Imaging techniques include plain films, CT, Ultrasound, and MRI. For diagnostic purposes, the division also performs plain films, arthrography, bone biopsies, and peripheral soft tissue lesions.
If you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.If you want genomic DNA, you crack your cells open in a different way and try to isolate as much of the stuff as possible.f you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.
The required gene is cut from a DNA molecule using a restriction enzyme. A bacterial plasmid is isolated and cut with the same restriction enzyme. This ensures cut ends are complementary (same base sequence) to the ends of the required gene. The required gene is joined to the plasmid using the enzyme DNA ligase in a process called ligation.