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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
The ubiquitous gram-negative facultative anaerobic bacteria P. aeruginosa is the causitive bacteria. It causes opportunistic infections. The bacteria is found routinely in soil and water sources, and grows and multiples rapidly in warm water. Improper maintenance of a hot tub can lead to the overgrowth of the bacteria. The bacteria is capable of producing a variety of pigments, the most well known being pyocyanin, which is a blue-green pigment. It is typically grown in the lab on blood agar, MacConkey agar or Pseudomonas isolation agar.