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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
Bacterial Solutions to Hydrogen Sulfide (H2S) Odors. Bacteria which consume H2S by Oxidation include: Thiobacillus denitrificans and Paracoccus pantotrophus (formerly Paracoccus denitrificans and Thiomicrospira pantotrophus) can function aerobically or anaerobically using nitrate as a source of oxygen.) Elemental sulfur, hydrogen sulfide, thiosulfate and polythionates can be degraded by T. concretivorus to sulfuric acid, which lowers the pH to around 2. 2) In a pH range of 5 to 7, Thiobacillus thioparis will establish itself and degrade hydrogen sulfide to thiosulfuric and polythionic acids.