Chest pain and shortness of breath if blood clots form in the blood vessels in your lungs and heart. Pain, redness, warmth, and swelling in the lower leg if blood clots form in the deep veins of your leg. Headaches, speech changes, paralysis (an inability to move), dizziness, and trouble speaking and understanding if blood clots form in the blood vessels in your brain. These signs and symptoms may indicate a stroke.
From Wikipedia, the free encyclopedia. A gene knockout (abbreviation: KO) is a genetic technique in which one of an organism 's genes is made inoperative (knocked out of the organism). knockout mouse (left) that is a model of obesity, compared with a normal mouse. A conditional knockout allows gene deletion in a tissue or time specific manner. This is done by introducing short sequences called loxP sites around the gene.
Knockouts are used to define the overall requirement for a gene and to model loss of function mutations. Knockins introduce heterologous coding sequences such as reporters or DNA recombinases, or to incorporate changes in DNA sequence to humanize a mouse gene or to generate point mutations.lternatively, DNA from C57BL/6J BAC genomic clones can be used to construct targeting vectors for a 129 ES cell line if there are no polymorphisms in the region of the gene between C57BL/6J and 129S1/SvImJ strains of mice.