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Meatloaf is done when a meat thermometer inserted in its center reads 160 degrees Fahrenheit. Ground beef used for food items like hamburgers or meatloaf should be at this temperature after it is cooked.
Bake the meatloaf in a preheated 350-degree F oven for 60 minutes, or until the internal temperature reaches 160 degrees. Let the loaf rest for 10 minutes before slicing, to allow the juices to redistribute and the loaf to firm up.