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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
Pseudomonas aeruginosa is a Gram-negative, rod-shaped, motile organism (polar flagella) which characteristically produce. water-soluble pigments which diffuse through the medium. The best known are pyocyanin (blue-green), pyoverdine (yellow-green,