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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
S. aureus would yield + to these tests, Methyl Red, Vogues-Prauskauer, Mannitol fermentation, while S. epidermidis won't. While an oxidase test (just add H2O2 to the colony), S. aureus would yield negative result and S. epidermidis would be positive.If you want to take it a step further you can look at sequences of the 16S and compare as that will give you what species you have.hile an oxidase test (just add H2O2 to the colony), S. aureus would yield negative result and S. epidermidis would be positive. If you want to take it a step further you can look at sequences of the 16S and compare as that will give you what species you have.