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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
398 P. H. Clarke bacteria which produce H2S from organic media, and that cystine is first reduced to cysteine. The numerous methods used to detect H,S production by micro-organisms vary with the source of sulphur and the metal salts used to indicate H2S formation.