Our MSK physicians specialize in bone and joint imaging, spinal imaging, metabolic bone disease, and osteoporosis. Imaging techniques include plain films, CT, Ultrasound, and MRI. For diagnostic purposes, the division also performs plain films, arthrography, bone biopsies, and peripheral soft tissue lesions.
If you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.If you want genomic DNA, you crack your cells open in a different way and try to isolate as much of the stuff as possible.f you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.
Restriction enzyme is used to cut open the plasmid. The DNA from a cell containing a gene of interest is isolated and inserted into the plasmid. The plasmid is put back into the bacterial cell and allowed to multiply with the gene of interest. This gene is then place in an organism where it is wanted, for example in a plant to create a pest resistant plant.