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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
Pseudomonas aeruginosa, a Gram-negative bacterium, is a common opportunist pathogen causing respiratory tract infections like pneumonia [5,6,18]. Generally, Gram-negative bacteria like P. aeruginosa are less permeable to QACs than non-sporulating, non-mycobacterial Gram-positive organisms [6,18]. The difference in uptake regulation by Gram-negative bacteria is mediated by structural differences in the outer membrane, porins, and efflux pumps [19,23].