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Inside the mitochondria is where most of your ATP are created, but some ATP can be made in the cytoplasm through a process called substrate-level phosphorylation. This is a process of forming ATP by the physical addition of a phosphate group to ADP.
During oxidative phosphorylation, electrons are transferred from electron donors to electron acceptors such as oxygen, in redox reactions.These redox reactions release energy, which is used to form ATP.In eukaryotes, these redox reactions are carried out by a series of protein complexes within the inner membrane of the cell's mitochondria, whereas, in prokaryotes, these proteins are located in the cells' intermembrane space.TP synthase, also called complex V, is the final enzyme in the oxidative phosphorylation pathway. This enzyme is found in all forms of life and functions in the same way in both prokaryotes and eukaryotes.