View Full Document. ï¬ 1  Primers determine the specificity of the PCR reaction. ï¬ The distance between the primer binding sites will determine the size of the PCR product. 2  T m ï¬ For short (14â20 bp) oligomers: ï¬ T m = 4Â° (GC) + 2Â° (AT)
Estrus, or heat, is the period in which the doe will stand and allow the buck to breed her. This phase of the reproductive cycle may last between 12 to 36 hours. The period from one heat cycle to the next is referred to as the estrous cycle. In goats, the estrous cycle occurs every 18 to 24 days, or 21 days on average.
In Animal Crossing. In Animal Crossing, the season occurs from October 15 - October 25. Four or five mushrooms can be found around the town starting from 8am. The mushroom can be sold to Tom Nook for a hefty price (see Mushroom). One mushroom disappears every 15 minutes , meaning that all mushrooms will disappear by 9:00 AM.