View Full Document. ï¬ 1  Primers determine the specificity of the PCR reaction. ï¬ The distance between the primer binding sites will determine the size of the PCR product. 2  T m ï¬ For short (14â20 bp) oligomers: ï¬ T m = 4Â° (GC) + 2Â° (AT)
Estrus, or heat, is the period in which the doe will stand and allow the buck to breed her. This phase of the reproductive cycle may last between 12 to 36 hours. The period from one heat cycle to the next is referred to as the estrous cycle. In goats, the estrous cycle occurs every 18 to 24 days, or 21 days on average.
During the rut elk frequently use areas around fresh water, and tend to bed in heavy timber five to six hours per day. A cow elk will remain in estrus for 12 to 15 hours, if they are not bred during this time frame they will normally have another estrus cycle 18 to 28 days later.