In bulk protein purification, a common first step to isolate proteins is precipitation with ammonium sulphate (NH 4) 2 SO 4. This is performed by adding increasing amounts of ammonium sulphate and collecting the different fractions of precipitate protein.rotein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.
Phenolic disinfectants are effective bactericides, fungicides, tuberculocides and virucides, but are ineffective against spore-forming bacteria such as Clostridium difficile. EPA-registered phenolic disinfectants are used to disinfect surface areas and non-critical medical devices.
Phenols and Related Compounds. Phenolic compounds used as antiseptics or disinfectants include pure phenol and substitution products with halogens and alkyl groups. They act to denature and coagulate proteins and are general protoplasmic poisons. Phenol (carbolic acid) is one of the oldest antiseptic agents. It is bacteriostatic at concentrations of 0.1%â1% and is bactericidal/fungicidal at 1%â2%. A 5% solution kills anthrax spores in 48 hr.