In bulk protein purification, a common first step to isolate proteins is precipitation with ammonium sulphate (NH 4) 2 SO 4. This is performed by adding increasing amounts of ammonium sulphate and collecting the different fractions of precipitate protein.rotein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.
Phenolic disinfectants are effective bactericides, fungicides, tuberculocides and virucides, but are ineffective against spore-forming bacteria such as Clostridium difficile. EPA-registered phenolic disinfectants are used to disinfect surface areas and non-critical medical devices.
1. Define the following terms: sterilization, disinfection, decontamination, disinfectant, antiseptic, and sanitizer. 2. State why chemical agents are usually unreliable for sterilization. 3. List five factors that may influence the antimicrobial action of disinfectants, antiseptics, and sanitizers. large number of such chemical agents are in common use. Some of the more common groups are listed below: 1. Phenol and phenol derivatives Phenol (5-10%) was the first disinfectant commonly used. However, because of its toxicity and odor, phenol derivatives (phenolics) are now generally used.