Yes it still harmful because it is air pollution to your lungs. My kid's pediatrician used to tell me that I should change my clothes after smoking a cigarette and before I carry my baby.
In Scrum, a product backlog item (PBI, backlog item, or item) is a unit of work small enough to be completed by a team in one Sprint iteration. Backlog items are decomposed into one or more tasks. See also backlog effort estimation unit.
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors.Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after the postdoctoral researchers who constructed it.The p stands for plasmid, and BR for Bolivar and Rodriguez..he plasmid has unique restriction sites for more than forty restriction enzymes. 11 of these 40 sites lie within the tetR gene. There are 2 sites for restriction enzymes HindIII and ClaI within the promoter of the tetR gene. There are 6 key restriction sites inside the ampR gene.