View Full Document. ï¬ 1  Primers determine the specificity of the PCR reaction. ï¬ The distance between the primer binding sites will determine the size of the PCR product. 2  T m ï¬ For short (14â20 bp) oligomers: ï¬ T m = 4Â° (GC) + 2Â° (AT)
Estrus, or heat, is the period in which the doe will stand and allow the buck to breed her. This phase of the reproductive cycle may last between 12 to 36 hours. The period from one heat cycle to the next is referred to as the estrous cycle. In goats, the estrous cycle occurs every 18 to 24 days, or 21 days on average.
the hedgehog breeding season can start from around april if we ve got enough energy post hibernation and the weather is warm enough and run until september however we are most active and amorous during the rut which is during may and june when the nights are nice and warm