Yes it still harmful because it is air pollution to your lungs. My kid's pediatrician used to tell me that I should change my clothes after smoking a cigarette and before I carry my baby.
In Scrum, a product backlog item (PBI, backlog item, or item) is a unit of work small enough to be completed by a team in one Sprint iteration. Backlog items are decomposed into one or more tasks. See also backlog effort estimation unit.
One of the most common plasmids used is the R-plasmid (or pBR322). This plasmid contains a replication origin, several recognition sequences for different restriction enzymes (with names like EcoRI), and two marker genes, which confer resistance to different antibiotics (ampicillin and tetracycline).enes can be cloned by cloning the bacterial cells that contain them, but this requires quite a lot of DNA in the first place. PCR can clone (or amplify) DNA samples as small as a single molecule. It is a newer technique, having been developed in 1983 by Kary Mullis, for which discovery he won the Nobel prize in 1993.