To make it clear what I'm talking about, I use a protocol like the following: 1  Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Meliodas is the captain of the Seven Deadly Sins and is the Dragon's Sin of Wrath. He is the owner of the renowned Boar Hat, and the main protagonist of the series. His Sacred Treasure is the demon-splitting sword, Lostvayne. Accelerator vs Meliodas.
Member Favorites: 2,822. Meliodas the leader of the Seven Deadly Sins and the Sin of Wrath with the symbol of the Dragon. He is the main protagonist of the Nanatsu no Taizai and the owner of the Boar Hat bar. Despite having the appearance of a child, Meliodas is actually much older, being at least 3000 years old.