View Full Document. ï¬ 1  Primers determine the specificity of the PCR reaction. ï¬ The distance between the primer binding sites will determine the size of the PCR product. 2  T m ï¬ For short (14â20 bp) oligomers: ï¬ T m = 4Â° (GC) + 2Â° (AT)
Estrus, or heat, is the period in which the doe will stand and allow the buck to breed her. This phase of the reproductive cycle may last between 12 to 36 hours. The period from one heat cycle to the next is referred to as the estrous cycle. In goats, the estrous cycle occurs every 18 to 24 days, or 21 days on average.
Goat does are seasonal breeders. They generally come into season monthly when the days shorten, usually during the fall through the winter months. Watch the calendar and your doe after she stands quietly for a buck and mates. If she does not return to estrus within 21 to 25 days, she is likely pregnant.