In bulk protein purification, a common first step to isolate proteins is precipitation with ammonium sulphate (NH 4) 2 SO 4. This is performed by adding increasing amounts of ammonium sulphate and collecting the different fractions of precipitate protein.rotein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.
Phenolic disinfectants are effective bactericides, fungicides, tuberculocides and virucides, but are ineffective against spore-forming bacteria such as Clostridium difficile. EPA-registered phenolic disinfectants are used to disinfect surface areas and non-critical medical devices.
Production of phenoloxidase by Cryptococcus neoformans in caffeic acid containing medium results in production of melanin pigments, which is incorporated into yeast wall resulting in brown colonies. Both the Gram stained smear and India ink wet mount of the colonies revealed yeast cells.