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FÃ¶rster resonance energy transfer. FÃ¶rster resonance energy transfer (FRET), fluorescence resonance energy transfer (FRET), resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores).
FRET can be an accurate measurement of molecular proximity at angstrom distances (10â100 Ã¥) and highly efficient if the donor and acceptor are positioned within the FÃ¶rster radius (the distance at which half the excitation energy of the donor is transferred to the acceptor, typically 3â6 nm).