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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
When grown on ordinary agar, the organism forms spherical smooth colonies about two to four millimeters in diameter. When grown on Hektoen enteric agar, colonies are bluish-green with black centres, indicating that the species does not ferment lactose (common to many Salmonella species).The use of Hektoen enteric agar is designed for detection of these types of bacteria. Figure 1.hen grown on Hektoen enteric agar, colonies are bluish-green with black centres, indicating that the species does not ferment lactose (common to many Salmonella species). The use of Hektoen enteric agar is designed for detection of these types of bacteria.