In which case, it depends on the type of inhibitor. A competitive inhibitor has a structure similar to the substrate, hence would bind to the active site as well, competing with the substrate for the enzyme active sites, decreasing enzymatic activity. A non-competitive inhibitor binds to the allosteric site of the enzyme, causing a structural change in the enzyme active site shape.
There's a difference between the effects of cold-brewed coffee vs. hot-brewed coffee: because different chemical reactions are achieved at higher temperatures, cold-brewed coffee will have a smoother caffeine effect and overall less acidity, which some people experience as part of the kick..
Coffee, regular or decaffeinated, is a well-known bladder irritant and can cause tremendous. discomfort for people who suffer from urinary tract disorders. Caffeine, because it is a Central. Nervous System stimulant, stimulates spasm in sensitive nerves and bladder muscles. Caffeine is. also a diuretic. The acidity of both regular and decaffeinated coffee is highly irritating to the. bladder and can contribute to the conditions that lead to cystitis.