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The Wagner tuba is a narrow-bore tuba with a French-horn mouthpiece. Tubas appeared first in Berlin in the 1820s, soon after the invention of the valve. They were soon accepted into the band and orchestra, displacing the serpent, ophicleide, and other such instruments of poorer tone quality and intonation.
The harmonica first appeared in Vienna, where harmonicas with chambers were sold before 1824 (see also Anton Reinlein and Anton Haeckl). Richter tuning, invented by Joseph Richter (who also is credited with inventing the blow and draw mechanism), was created in 1826 and was eventually adopted nearly universally.