For Best Results. Apply the Upper Brush against the top lashes and sweep from root to tip until a clean, voluminous look is achieved, followed by the lower lash line using the Micro Brush. Do not let dry between coats. Removes with soap and water or with MaybellineÂ® Clean Express!Â® Eye Makeup Remover.
Sonication can be used to enhance solubility. 1  First, assign a value of â1 to each acidic residue (Asp [D], Glu [E], and the C-terminal âCOOH). 2  If the overall charge of the peptide is positive, the peptide is basic. 3  If the overall charge of the peptide is negative, the peptide is acidic. Acidic peptides might be soluble in PBS (pH 7.4). If this fails, add a small amount of basic solvent such as 0.1 M ammonium bicarbonate to dissolve the peptide, and then add water to the desired concentration.
The peptide can be diluted later with the assayâs buffer, but if the buffer is used initially and peptide fails to dissolve, recovery of the pure peptide free of nonvolatile salts and organic solvents can be difficult. If the peptide persists as visible particles, sonication can be tried.