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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
Brownish-black colonies surrounded by a black zone are positive. Oxgall (bile) is inhibitory agent. Ferric citrate is indicator. Blood Agar (BAP): consists of a basal medium such as TSA enriched with 5% defibrinated sheep blood or in some locations, horse blood.