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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
Methylene Blue (EMB) Agar is a medium in which the dyes eosin and methylene blue inhibit Gram. positive bacteria. In addition, lactose fermenters will lower the pH of the agar, facilitating their uptake of. the dyes, which causes them to display nucleated colonies with dark centers. MacConkey (MAC) Agar. contains bile salts and crystal violet dye to inhibit the growth of most Gram positive bacteria and neutral.