According to career resource website Work911, using techniques such as open-ended questioning (i.e., asking questions that require more than a yes or no answer) can allow all parties of a conversation to give their opinions on a subject. This technique can make verbal communication among team members more effective.
Immunofixation fixes certain proteins into place with antibodies and then washes away the others before staining them. On a computer screen, these proteins form a pattern of bands. Peaks and valleys in the bands may mean that you may be making too many or too few of certain proteins.
From this step on there are two commonly used detection systems, immunofluorescence or immunoperoxidase. For the immunofluorescence method, slides are next incubated with 10% normal goat serum for 37oC for 45 min to block nonspecific binding. This is followed by incubation with an appropriate dilution of adduct specific antibody in blocking solution for 45 min at 37oC or at 4oC overnight.