For Best Results. Apply the Upper Brush against the top lashes and sweep from root to tip until a clean, voluminous look is achieved, followed by the lower lash line using the Micro Brush. Do not let dry between coats. Removes with soap and water or with MaybellineÂ® Clean Express!Â® Eye Makeup Remover.
Sonication can be used to enhance solubility. 1  First, assign a value of â1 to each acidic residue (Asp [D], Glu [E], and the C-terminal âCOOH). 2  If the overall charge of the peptide is positive, the peptide is basic. 3  If the overall charge of the peptide is negative, the peptide is acidic. Acidic peptides might be soluble in PBS (pH 7.4). If this fails, add a small amount of basic solvent such as 0.1 M ammonium bicarbonate to dissolve the peptide, and then add water to the desired concentration.
Aqueous solution: Dilution with D5W (250 or 500 mL) is required prior to administration. Lyophilized powder: Use only SWFI or D5W to reconstitute powder. To obtain final concentration of 5 mg/mL add 10 mL of diluent to 50 mg vial or 20 mL diluent to 100 mg vial. Gently swirl vial to dissolve powder. Dilution with D5W (250 or 500 mL) is required prior to administration.