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There are 56 calories in a 1oz serving of homemade beef jerky. Calorie breakdown: 18% fat, 2% carbs, 80% protein.
Calories and Fat. A 1-ounce portion of beef jerky contains 116 calories and 7.26 grams of fat, of which 3 grams are saturated. While beef jerky also contains heart-healthy monounsaturated fat, much of the fat in the food is saturated. If your diet contains large amounts of saturated fat, you're at an increased risk for heart disease. A diet high in saturated fat can boost your cholesterol levels.