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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
1 Principles of the Procedure Mannitol Salt Agar is a nutritive medium due to its content of peptones and beef extract, which supply essential growth factors, such as nitrogen, carbon, sulfur and trace nutrients.oagulase-positive staphylococci produce growth of yellow colonies with yellow zones. Coagu-lase negative staphylococci produce small red colonies with no color change to the medium. Micrococcus produce large, white to orange colonies, with no color change to the medium. Most other bacteria will be inhibited.