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Vial: Once the vial is punctured, it is OPEN. Once you stick a needle in the vial, it is OPEN. OPEN vials can be stored in the fridge or at CONTROLLED room temperature. Regardless of where it is stored, OPEN insulin will only last 28 days before it must be thrown in the trash.
1 It is ok to keep a bottle of insulin you are using at room temperature for up to 28 days (room temperature is 59 to 86 F). The preservative in insulin keeps it from spoiling this long.  Insulin at room temperature injected into the skin is more comfortable for many people.