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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
A common application of this stain is in the preparation of EMB agar, a differential microbiological medium, which inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that do not (e.g., Salmonella, Shigella).he pattern of lactose fermentation and H 2 S production aids in the identification of the organism subbed to the plate. HE agar is designed to isolate and recover fecal bacteria belonging to the Enterobacteriaceae family. HE is particularly useful in isolating Salmonella and Shigella.