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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
Using neutral red pH indicator, the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-). This medium is also known as an indicator medium and a low selective medium.Presence of bile salts inhibits swarming by Proteus species.t contains bile salts (to inhibit most Gram-positive bacteria), crystal violet dye (which also inhibits certain Gram-positive bacteria), neutral red dye (which turns pink if the microbes are fermenting lactose). Composition: 1  Peptone - 17 g. 2  Proteose peptone - 3 g.