In which case, it depends on the type of inhibitor. A competitive inhibitor has a structure similar to the substrate, hence would bind to the active site as well, competing with the substrate for the enzyme active sites, decreasing enzymatic activity. A non-competitive inhibitor binds to the allosteric site of the enzyme, causing a structural change in the enzyme active site shape.
There's a difference between the effects of cold-brewed coffee vs. hot-brewed coffee: because different chemical reactions are achieved at higher temperatures, cold-brewed coffee will have a smoother caffeine effect and overall less acidity, which some people experience as part of the kick..
Coffee, regular or decaffeinated, is a well-known bladder irritant and can cause tremendous. discomfort for people who suffer from urinary tract disorders. Caffeine, because it is a Central. Nervous System stimulant, stimulates spasm in sensitive nerves and bladder muscles. Caffeine is. also a diuretic. The acidity of both regular and decaffeinated coffee is highly irritating to the. bladder and can contribute to the conditions that lead to cystitis. For people who have interstitial. cystitis (IC), drinking just one cup of coffee, even decaffeinated coffee, can trigger hours of pain.