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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
Nutrient Agar is a complex medium because it contains ingredients with contain unknown amounts or types of nutrients. Nutrient Agar contains Beef Extract, Peptone and Agar in water. Beef extract is the commercially prepared dehydrated form of autolysed beef and is supplied in the form of a paste.