Actinic keratoses that develop on the lips are referred to as actinic cheilitis. The clinical appearance of an actinic keratosis is that of a small (several millimeters), red scaly papule or plaque, with induration and thickening of the epidermis.
1 Location: Ideally, the probe should be in close proximity to the forward or reverse primer, but should not overlap with a primer-binding site on the same strand. 2  Probes can be designed to bind to either strand of the target. 3  Melting temperature (Tm): Preferably, probes should have a Tm 6â8Â°C higher than the primers.
1 Probes can be designed to bind to either strand of the target. 2  Melting temperature (Tm): Preferably, probes should have a Tm 6â8Â°C higher than the primers. 3  If the melting temperature is too low, the percentage of probe bound to target will be low.