However, use of the gal is deprecated by ISO 80000-3:2006. The gal is a derived unit, defined in terms of the centimeterâgramâsecond (CGS) base unit of length, the centimeter, and the second, which is the base unit of time in both the CGS and the modern SI system.
It has been shown experimentally that if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will increase until it reaches a maximum. After this point, increases in substrate concentration will not increase the velocity (delta A/delta T). This is represented graphically in Figure 8. It is theorized that when this maximum velocity had been reached, all of the available enzyme has been converted to ES, the enzyme substrate complex.
3 at a high enough level. At this point an increase in substrate concentration will not have a considerable effect on the rate of the reaction. The rate of the reaction will only be affected through other means, such as temperature or pH changes. This phenomenon is depicted through the Michaelis-Menten equation. As the concentration of the substrate increases the rate of the reaction asymptotically approaches its maximum velocity (V max). The concentration of the substrate at which the rate of the reaction is one half the V max is known as the Michaelis constant (K M). The graph of this relationship as a function of substrate concentration is curvilinear and it plateaus as it approaches V max.