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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms.It typically contains (mass/volume): 1  0.5% Peptone. 2  0.3% beef extract / yeast extract.3  1.5% agar. 4  0.5% NaCl. 5  distilled water. 6  pH adjusted to neutral (6.8) at 25 Â°C.utrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 1  0.5% Peptone. 2  0.3% beef extract / yeast extract. 3  1.5% agar. 4  0.5% NaCl. 5  distilled water. 6  pH adjusted to neutral (6.8) at 25 Â°C.