Anglers also need to be aware of license costs: for residents, $8.50 whether fishing fresh or tidal waters, but priced higher for non-residents who will fish either fresh or salt water. The Delaware license for both fresh and tidal waters is also valid for recreational crabbing and clamming.
RNA and Protein Synthesis. During transcription, the enzyme RNA polymerase synthesizes a strand of messenger RNA (mRNA) using one strand of the gene as a template.mRNA is synthesized using nucleotides that contain the bases A, C, G, and U (instead of T), which pair with the complementary bases of the DNA template strand.uring transcription, the enzyme RNA polymerase synthesizes a strand of messenger RNA (mRNA) using one strand of the gene as a template.
1 At this step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTPs that are complementary to the template in 5' to 3' direction, condensing the 5'- phosphate group of the dNTPs with the 3'- hydroxyl group at the end of the nascent (extending) DNA strand. If the genomic DNA sequence of a gene is known, RT-PCR can be used to map the location of exons and introns in the gene. 2  The 5' end of a gene (corresponding to the transcription start site) is typically identified by RACE-PCR (Rapid Amplification of cDNA Ends).