Enzymes have active sites where they come into contact with particular substrates. The catalytic properties of enzymes are a cyclic process. Once a substrate has come into contact with the active site of an enzyme, it is modified by the enzyme to form the end product.
1 Reducing saturated fat to no more than 5 to 6 percent of total calories. 2  For someone eating 2,000 calories a day thatâs about 11 to 13 grams of saturated fat. 3  Reducing the percent of calories from trans fat.
Because fats have 9 calories per gram, you can have up to 24 grams of saturated fat in one day. You also need to consume no more than 300 milligrams of dietary cholesterol on a daily basis. As an example, one 3-ounce sirloin steak contains nearly 3.5 grams of saturated fat and 75 milligrams of cholesterol.