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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
Nutrient Broth. Nutrient broth is made up of water, beef extract, and peptone. Peptone is a nitrogen mixture of plant and animal sources. Agar is then added to make the mixture into a solid and is called nutrient agar.n order to do this in the most effective way, it is important to use a growth medium that is as close as possible to the natural environment. Nutrient broth with agar is called nutrient agar. The overall purpose of the agar is to customize the media for the specific bacteria.