Actinic keratoses that develop on the lips are referred to as actinic cheilitis. The clinical appearance of an actinic keratosis is that of a small (several millimeters), red scaly papule or plaque, with induration and thickening of the epidermis.
1 Location: Ideally, the probe should be in close proximity to the forward or reverse primer, but should not overlap with a primer-binding site on the same strand. 2  Probes can be designed to bind to either strand of the target. 3  Melting temperature (Tm): Preferably, probes should have a Tm 6â8Â°C higher than the primers.
Since a decrease in concentration usually results in a decreased reaction rate, the forward reaction slows down. Initially there is no hydrogen iodide present so the reverse reaction does not occur. However, as the forward reaction proceeds the concentration of hydrogen iodide increases (see graph below).