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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
Cortisol is a corticosteroid hormone that is released by your adrenal gland during stressful situations. When the stress does not last very long, the levels of cortisol in your brain dissipate.