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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
Blood Test for Nicotine. Nicotine presence can be detected in the blood for a short period only (about 48 hours) . However, its metabolite, cotinine, can be detected up to about three weeks from the last use and the cut-off detection levels is 200 ng/ml.onetheless, it can be detected in the saliva for approximately 24 hours after the last smoke. Its metabolite, cotinine, can be detected for as long as 4 to 7 days depending on the amount of nicotine consumed. For heavy smokers, it may be detected up to seven to fourteen days.