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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
In detecting nicotine and its metabolites in the body, levels remain detectable in the urine only for 3 to 4 days. [ 8,18 ]. However, this may be different for some cases. For passive smokers, urine nicotine test can be positive for as long as 15 to 20 days.