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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
through a microscope lens with a NA of 1, then the resolution is 500 nm / 2 x 1, or 250 nm. This means that you can see 2 objects that are at least 250 nm apart as 2 distinct objects; objects closer together than 250 nm will appear fuzzy or as one object.