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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
1 Prolactin, a hormone that stimulates breast development and milk production during pregnancy, may cause the breast tenderness associated with PMS.  Cortisol is a hormone involved in the body's stress response. An excess of cortisol can cause anxiety and insomnia, whereas a deficiency can lead to depression and lethargy. One study found that women with PMS-related depression had an imbalance of cortisol levels compared to women who had few or no PMS symptoms.