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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
Cortisol is a corticosteroid hormone produced by the zona fasciculata of the adrenal cortex (in the adrenal gland). It is a vital hormone that is often referred to as the stress hormone as it is involved in the response to physical and psychological stress.