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TRH stimulation test. Prior to the availability of sensitive TSH assays, thyrotropin releasing hormone (TRH) stimulation tests were relied upon for confirming and assessing the degree of suppression in suspected hyperthyroidism. Typically, this stimulation test involves determining basal TSH levels and levels 15 to 30 minutes after an intravenous bolus of TRH.
Thyrotropin-releasing hormone (TRH), also called thyrotropin-releasing factor (TRF), thyroliberin or protirelin, is a tropic, tripeptidal hormone, produced by the hypothalamus, that stimulates the release of TSH (thyroid-stimulating hormone) and prolactin from the anterior pituitary.RH is produced by the hypothalamus in medial neurons of the paraventricular nucleus. At the beginning, it is synthesized as a 242-amino acid precursor polypeptide that contains 6 copies of the sequence-Gln-His-Pro-Gly-, flanked by Lys-Arg or Arg-Arg sequences.