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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
The length of time nicotine remains in the blood stream depends on the amount consumed and the frequency of consumption. Nicotine can stay for 48 hours. Beyond this time it is not detected either in the blood or urine. However, the cotinine metabolite can leave traces for about 7 days.