Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
All isolates were positive for metachromatic granules and none of these showed capsule or endospore formation (Table 3). Endospore staining was carried out by heating the air dried bacterial smear with malachite green for 5 min, followed by rinsing with water and counterstaining with safranin for 30s.