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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
M TOT VIS: Total visual magnification of the microscope If in the above equation the total visual magnification is replaced by the relationship of useful magnification (M TOT VIS = 500 to 1,000 x NA), it can be seen that, to a first approximation, the depth of field is inversely proportional to the square of the numerical aperture.