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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
From Wikipedia, the free encyclopedia. The numerical aperture with respect to a point P depends on the half-angle, Î¸1, of the maximum cone of light that can enter or exit the lens and the ambient index of refraction. As a pencil of light goes through a flat plane of glass, its half-angle changes to Î¸2. NA = n 1 sin â¡ Î¸ 1 = n 2 sin â¡ Î¸ 2 .