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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
Cortisol is a hormone your body releases when youâre under stress. Your doctor may want you to be tested to see if you have the right level of cortisol in your blood. Cortisol is made by your adrenal glands-- two small glands that sit on top of your kidneys.