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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
This substance remains in urine for around 3 to 50 days during which nicotine content is detectable. A regular urine sample is enough to show the presence of nicotine in urine. Even in case of passive smokers, nicotine in the urine can be detected for 20-25 days at the maximum. Nicotine is only harmful for human in higher dose such as 30 to 60 milligram. However, it is not harmful when it is present in less quantity.