Under usual conditions, cells infected with wild type radixin, nonphosphorylatable radixin-T564A, and radixin-T564D (active phospho-mimicking mutant) were found to be heavily expressed in canalicular membrane compartment vacuoles, typically colocalizing with Mrp-2.
After hypoxia for 24 hours, both endogenous and overexpressed wild type radixin and the radixin-T564A mutant were found to be translocated to the cytoplasmic space.