In the same assays, MPP8 displayed no binding to either H3 peptides harbouring methylated or unmethylated K27 (18-37aa) (Figure 2C) or H3 chemically installed with different methyl-K27 analogs (Supplementary Figure S1B).
These data clearly demonstrate that MPP8 speci- fically binds to methyl-H3K9 in vitro and its binding ability requires an intact chromodomain.