Given that lin-35; slr-2 mutants arrest because of a defect in nutrient utilization, we tested whether prohibitin knockdown could also alter fat storage at the L1 stage of arrest.
Lin-35; slr-2 treated with RNAi of phb-1 or phb-2 showed a dramatic decrease in L1 fat stores based on staining with Oil Red O (Figure 3A).