In culturing , 100 mL of MRS medium was added to a 500 mL Sakaguchi flask , 100 mL of the above culture solution was inoculated, and the medium was shaken 100 times/min.
Lactobacillus sakei strain JCM1157, which does not produce bacteriocin, was employed as an indicator strain and Lactobacilli AOAC medium was layered.