next, small , 110 nm diameter, unilamellar vesicles of popc doped with 1 mol% gm1 are prepared and deposited onto these patterned substrates by vesicle fusion9 , 10.
Vesicles are prepared by mechanical extrusion in water and the resulting vesicular solution adjusted with phosphate buffered saline (PBS) to produce the salt concentration of 75 mM.