Hemoglobinuria is not necessary for diagnosis because hemoglobinuria is sometimes absent in the case. Additionally, a history of exposure to cold temperatures is not always attained. Given the fact that hemoglobinuria as well as a personal history of exposure to cold temperatures are not always present, the diagnosis heavily relies on laboratory testing. The laboratory tests comprise complete blood count and peripheral blood smear. The laboratory test results will reveal evaluations like anisocytosis, nucleated red blood cells, poikilocytosis, polychromasia, spherocytosis, and erythrophagocytosis by neutrophils. Blood typing is supposed to be performed with every patient even if their anemia is mild since the hemoglobin can fall all of a sudden and require prompt blood transfusion. Anti-immunoglobulin G (anti-Ig) often disassociates itself from the surface of red blood cells under warm degrees of temperatures. Thus, the direct antiglobulin test for anti-immunoglobulin G (anti-Ig) often manifests a negative results. That's why it's important to note that the indirect antiglobulin test must be carried out in an environment at cold temperatures. According to Medscape, the so-called<unk><unk>  bithermic hemolytic test is an assay of hemolysis where the serum of the patient goes incubated with normal complement and red blood cells under to to permit the components in the initial stage of complement to be settled. Afterwards, the specimen goes incubated under so that the later components of complement can then be enabled. The membrane attack complex leads the red blood cells to undergo lysis. Blood chemistry, serology, urinalysis and suchlike may also be performed. For patients of DLHA who perform the serologic testing, summaries for syphilis, mycoplasmal infection, or viruses such as, adenovirus, cytomegalovirus, Epstein-Barr virus, influenza A, measles, mumps, and varicella might be shown positive. It's up to the underlying pathological conditions.
Donath–Landsteiner hemolytic anemia
Warm antibody autoimmune hemolytic anemia
Cold autoimmune hemolytic anemia
symptom of Congenital dyserythropoietic anemia
medical cause of Hemolytic anemia
Sickle cell-beta thalassemia
Twin anemia-polycythemia sequence
Hughes–Stovin syndrome