Fig 1.<unk> <unk>  (DTS) is a bioinformatics method to detect the presence of novel pathogen transcripts through computational removal of the host sequences. DTS is the direct in silico analogue of the wet-lab approach Representational Difference Analysis (RDA), and is made possible by unbiased high-throughput sequencing and the availability of a high-quality, annotated reference genome of the host. The method specifically examines the etiological agent of infectious diseases and is best known for discovering Merkel cell polymavirus, the suspect causative agent in Merkel cell carcinoma.
Digital transcriptome subtraction
Yeast expression platform
Next-generation matrix
Electrochemical aptamer-based biosensors
Kozak consensus sequence
Triage tag
Epigenomics
Micropore particle technology