Oxidation of<unk>  derivatives to fluorescent thiochromes by potassium ferricyanide under alkaline conditions A positive diagnosis test for<unk>  involves measuring the activity of the enzyme transketolase in erythrocytes (Erythrocyte transketolase activation assay). Alternatively,<unk>  and its phosphorylated derivatives can directly be detected in whole blood, tissues, foods, animal feed, and pharmaceutical preparations following the conversion of<unk>  to fluorescent thiochrome derivatives (thiochrome assay) and separation by high-performance liquid chromatography (HPLC). Capillary electrophoresis (CE) techniques and in-capillary enzyme reaction methods have emerged as alternative techniques in quantifying and monitoring<unk>  levels in samples. The normal<unk>  concentration in EDTA-blood is about 20-100 µg/l.
Thiamine deficiency
Fluorine deficiency
Boron deficiency 
Selenium deficiency
Biotin deficiency
Chromium deficiency
Copper deficiency
Magnesium deficiency