In molecular biology,<unk>  <unk>  is a multi-subunit DNA binding complex (6 subunits) that binds in all eukaryotes and archaea in an ATP-dependent manner to<unk> s of replication. The subunits of this complex are encoded by the<unk> 1,<unk> 2,<unk> 3,<unk> 4,<unk> 5 and<unk> 6 genes.<unk>  is a central component for eukaryotic DNA replication, and remains bound to chromatin at replication<unk> s throughout the cell cycle.<unk>  directs DNA replication throughout the genome and is required for its initiation.<unk>  and Noc3p bound at replication<unk> s serve as the foundation for assembly of the pre-replication complex (pre-RC), which includes Cdc6, Tah11 (a.k.a. Cdt1), and the Mcm2-Mcm7 complex. Pre-RC assembly during G1 is required for replication licensing of chromosomes prior to DNA synthesis during S phase. Cell cycle-regulated phosphorylation of<unk> 2,<unk> 6, Cdc6, and MCM by the cyclin-dependent protein kinase Cdc28 regulates initiation of DNA replication, including blocking reinitiation in G2/M phase. The<unk>  is present throughout the cell cycle bound to replication<unk> s, but is only active in late mitosis and early G1. In yeast,<unk>  also plays a role in the establishment of silencing at the mating-type loci Hidden MAT Left (HML) and Hidden MAT Right (HMR).<unk>  participates in the assembly of transcriptionally silent chromatin at HML and HMR by recruiting the Sir1 silencing protein to the HML and HMR silencers. Both<unk> 1 and<unk> 5 bind ATP, though only<unk> 1 has ATPase activity. The binding of ATP by<unk> 1 is required for<unk>  binding to DNA and is essential for cell viability. The ATPase activity of<unk> 1 is involved in formation of the pre-RC. ATP binding by<unk> 5 is crucial for the stability of<unk>  as a whole. Only the<unk> 1-5 subunits are required for<unk>  binding;<unk> 6 is essential for maintenance of pre-RCs once formed. Interactions within<unk>  suggest that<unk> 2-3-6 may form a core complex. In addition, a recent report suggests that budding yeast<unk>  dimerizes in a cell cycle dependent manner to control licensing.
Origin recognition complex
Mutanome
Bioptome
Cis-regulatory element
Sp1 transcription factor
Transfersome
Pre-integration complex
Cubosome