The overall reaction catalysed by the<unk> s is: * RCHO + NAD+ + H2O → RCOOH + NADH + H+ In this NAD(P)+-dependent reaction, the<unk> yde enters the active site through a channel extending from the surface of the enzyme. The active site contains a Rossmann fold, and interactions between the cofactor and the fold allow for the action of the active site. Mechanism of<unk>  A sulfur from a cysteine in the active site makes a nucleophilic attack on the carbonyl carbon of the<unk> yde. The hydrogen is kicked off as a hydride and attacks NAD(P)+ to make NAD(P)H. The enzyme's active site then goes through an isomorphic change whereby the NAD(P)H is moved, creating room for a water molecule to access the substrate. The water is primed by a glutamate in the active site, and the water makes a nucleophilic attack on the carbonyl carbon, kicking off the sulfur as a leaving group.
Aldehyde dehydrogenase
Glutamate dehydrogenase
Transaminase
Aldehyde oxidase
Xanthine oxidase
Pyruvate carboxylase
Acyl-CoA dehydrogenase
Butyrate esterase