May-Grünwald-Giemsa The May-Grünwald-Giemsa stain is a two step procedure that includes first staining with May-Grünwald stain, which does not produce the<unk>  effect, followed by staining the Giemsa stain which does produce the<unk>  effect. Wright's and Wright-Giemsa stains Wright's stain can be used alone or in combination with the Giemsa stain, which is known as the Wright-Giemsa stain. Wright's stain is named after James Homer Wright who in 1902 published a method using heat to produce polychromed methylene blue, which is combined with eosin Y. The polychromed methylene blue is combined with eosin and allowed to precipitate, forming an eosinate which is redissolved in methanol. The addition of Giemsa to Wright's stain increases the brightness of the "reddish-purple" color of the cytoplasmic granules. The Wright's and Wright-Giemsa stains are two of the<unk> -type stains in common use in the United States and are mainly used for the staining of blood and bone marrow films. Leishman stain In 1901 William Leishman developed a stain that was similar to Louis Jenner's but with the replacement of pure methylene blue with polychromed methylene blue. Leishman's stain is prepared from the eosinate of polychromed methylene blue and eosin Y using methanol as the solvent. Giemsa stain Giemsa stain is composed of "Azure II" and eosin Y with methanol and glycerol as the solvent. "Azure II" is thought to be a mixture of azure B (which Giemsa called "azure I") and methylene blue, although the exact composition of "azure I" is considered a trade secret. Comparable formulations using known dyes have been published and are commercially available. Giemsa stain is considered to be the standard stain for detection and identification of the malaria parasite.
Romanowsky stain
Wayson stain
Bielschowsky stain
Auramine phenol stain
Movat's stain
Feulgen stain
Jones' stain
Alcian blue stain