In terms of brain imaging, the ability for<unk>  imaging to reveal specific structures in such unobstructed detail has led to promising avenues of future applications including local circuit wiring (especially as it relates to the Connectome Project), relationships between neural cells, roles of subcellular structures, better understanding of protein complexes, and imaging of nucleic acids and neurotransmitters. An example of a discovery made through<unk>  imaging is a peculiar 'ladder' pattern where neurons connected back to themselves and their neighbors, which has been observed in animals to be connected to autism-like behaviors. The<unk>  technique has expanded to several applications beyond the brain. Numerous modifications have been published to build upon the initial publications and efforts have been made both academically and within the biotech industry to apply broad-scope applications to<unk> .<unk>  continues to gain traction as an emergent technology that can provide powerful insights into clinical diagnostics in the future.<unk>  can be used with little or no modifications to clear most other organs such as liver, pancreas, spleen, testis, and ovaries and other species such as zebrafish. While bone requires a simple decalcification step, similarly, plant tissue requires an enzymatic degradation of the cell wall. NIH director Francis Collins has already expressed his hopes for this emergent technology, saying:
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