# Unroof vesicle and scrape base w/ sterile №15 scalpel blade # Smear onto a clean glass slide # Fix w/ gentle heat or air dry # Fix w/ MeOH (Methanol) # Stain w/ Giemsa, methylene blue or Wright’s stain. # Microscopic examination using an oil immersion lens. (Look for multinucleated giant cells) A modified test can be performed using proprietary agents which requires fewer steps and allows the sample to be fixed quicker.
Tzanck test
Schick test
Nardi test
Lamellar body count
Holtzman inkblot technique
Vinegar test
Elek's test
Buehler test