Proteins separated by SDS-PAGE, Coomassie brilliant blue staining<unk> s <unk> s) are<unk> s of immunoglobulins that are seen when a patient's blood serum, or cerebrospinal fluid (CSF) is analyzed. They are used in the diagnosis of various neurological and blood diseases, especially in multiple sclerosis. Two methods of analysis are possible: (a) protein electrophoresis, a method of analyzing the composition of fluids, also known as "agarose gel electrophoresis/Coomassie blue staining", and (b) the combination of isoelectric focusing/silver staining. The latter is more sensitive. For the analysis of cerebrospinal fluid, a patient has a lumbar puncture performed, which collects some of his or her cerebrospinal fluid. The blood serum can be gained from a clotted blood sample. Normally it is assumed that all the proteins that appear in the CSF, but are not present in the serum, are produced intrathecally (inside the CNS). Therefore, it is normal to subtract<unk> s in serum from<unk> s in CSF when investigating CNS diseases.
Oligoclonal band
Antiganglioside antibodies
Lymphocytic pleocytosis
Lymphoproliferative response
Monocytosis
Monoclonal B-cell lymphocytosis
Anti-MAG peripheral neuropathy
Isoantibodies