Specimen: Fresh stool is collected. Culture: Specimen is inoculated on selective media like MacConkey's agar, DCA, XLD agar. Selenite F broth(0.4%) is used as enrichment medium which permits the rapid growth of enteric pathogens while inhibiting the growth of normal flora like E. coli for 6–8 hours. Subculture is done on the solid media from selenite F broth. All the solid media are incubated at 37 degrees for 24 hours. Cultural characteristics: Colorless (NLF) colonies appear on MacConkey's agar which are further confirmed by gram staining, hanging drop preparation and biochemical reactions.
Bacillary dysentery
Dysentery
Travelers' diarrhea
Porcine epidemic diarrhoea
Rotaviral gastroenteritis
Listeriosis
Shigellosis
Bartonellosis