The<unk><unk>  or acetic anhydride test is used for the detection of cholesterol. The formation of a green or green-blue colour after a few minutes is positive.<unk><unk>  is a reagent used in a colourimetric test to detect cholesterol, which gives a deep green colour. This colour begins as a purplish, pink colour and progresses through to a light green then very dark green colour. The colour is due to the hydroxyl group (-OH) of cholesterol reacting with the reagents and increasing the conjugation of the un-saturation in the adjacent fused ring. Since this test uses acetic anhydride and sulfuric acid as reagents, caution must be exercised so as not to receive severe burns. Method: Dissolve one or two crystals of cholesterol in dry chloroform in a dry test tube. Add several drops of acetic anhydride and then 2 drops of concentrated H2SO4 and mix carefully. After the reaction is finished, the concentration of cholesterol can be measured using spectrophotometry.
Liebermann–Burchard test
Vinegar test
Beutler test
Casoni test
Kurzrock–Miller test
Galeazzi test
Gaenslen's test
Wassermann test