Origin of<unk>  antigenes According to the developers’ publications, vaccine antigens are three peptides of the spike protein and a chimeric protein consisting of two parts (viral nucleocapsid protein and bacterial maltose-binding protein). In addition, the polyhistidine-tag - a short peptide that is introduced into a vaccine composition to purify a chimeric protein from a bacterial lysate - is also a vaccine antigen against which antibodies can form in those who have received the vaccine. A person vaccinated with<unk>  can develop antibodies not only to the peptides of the spike protein, but also to other antigens present in the vaccine. According to Anna Popova, head of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare, it takes 42 days for those vaccinated with<unk>  to develop immunity. The vaccine includes three chemically synthesized short fragments of the viral spike protein - peptides, which, according to the developers of<unk>  represent the protein regions containing B-cell epitopes that should be recognized by the human immune system. These peptides are represented by following amino acid sequences: 1) CRLFRKSNLKPFERDISTEIYQAGS, 2) CKEIDRLNEVAKNLNESLIDLQE, 3) CKNLNESLIDLQELGKYEQYIK. In the vaccine all peptides are conjugated to a carrier protein, which is an expression product of the chimeric gene. This chimeric gene was created by fusion of two genes originating from different organisms, namely a gene encoding a viral nucleocapsid protein and a gene encoding a bacterial maltose-binding protein (MBP). The fusion chimeric gene expressed in Escherichia coli. The sequence of the chimeric protein is available from the patent. The genetic construct of the chimeric gene also includes a short genetic fragment encoding a polyhistidine-tag, which is used to purify the chimeric protein from E. coli lysate. After the purification, the protein is conjugated with three peptides in a way that only one variant of the peptide molecule is attached to each protein molecule. As a result, three types of conjugated molecules are created: chimeric protein with attached peptide number 1, the same protein with peptide number 2, and finally the same protein with peptide number 3. All three types of conjugated molecules are included in the vaccine.<unk> : antigens origin and composition
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