<unk>  is a pre-prepared non-porous plastic reagent strip with a predefined gradient of antibiotic, covering a continuous concentration range. It is applied to the surface of an agar plate inoculated with the<unk>  strain, where there is release of the antimicrobial gradient from the plastic carrier to the agar to form a stable and continuous gradient beneath and in nearby to the strip. The time taken for a plate to be ready depends on the bacterium that is being<unk> ed, and the conditions of the agar plate. The predefined<unk>  gradient remains stable for at least 18 to 24 hours; that is, a period that covers the critical times of many species of fastidious and non-fastidious organisms. After the<unk> , the bacterial growth becomes visible after incubation and a symmetrical inhibition ellipse centered along the strip is seen. The MIC value is read from the scale in terms of µg/mL where the ellipse edge intersects the strip. After the required incubation period, the minimum inhibitory value is read where the edge of the inhibition ellipse intersects the side of the strip. The plate should not be read if the culture appears mixed or if the lawn of growth is too light or too heavy.<unk>  MIC endpoints are usually clear-cut although different growth/inhibition patterns may be seen depending on the antifungal or antibiotic used.<unk>  being used to determine the susceptibility of Candida albicans to caspofungin. Selection of agar medium<unk>  can be used with many different kinds of AST agar medium as long as the medium supports good growth of the<unk>  organism and does not interfere with the activity of the antimicrobial agent. However, to maximise reproducibility, the medium chosen should fulfil the basic requirements for a susceptibility<unk>  medium. The following AST media are recommended for use with<unk> : * Aerobes: Mueller Hinton agar such as MHE (bioMérieux) * Anaerobes: Brucella blood agar with appropriate supplements These media may require supplemental nutrients to obtain enhanced growth of nutritionally fastidious organisms such as pneumococci, streptococci, Abiotrophia, Haemophilus, gonococci, meningococci and Campylobacter. In general, media recommendations from the Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility<unk> ing (EUCAST) are considered appropriate for<unk> .
Etest
Rape statistics
Minor test
Kick chart
Projective test
E chart
Bioanalysis
Test panel