Crossed<unk>  of 2 microLitre of normal human serum. The electrophoresis was performed in thin layers of agarose, the pictured gel is about 7x7 cm. The lower part is the first dimension gel without antibodies, where the serum was applied into the slot at the lower left. The upper part is the second dimension gel with Dako antibodies against human serum proteins. More than 50 major serum proteins can be named. Fused rocket<unk>  of an affinity chromatographic separation of human serum proteins on con A. A 15 microlitre sample from each fraction is applied (starting from left) and let to diffuse about one hour, then electrophorsis was performed over night. Peak "a" is unretained, peak "b" is retained and eluted with methyl-mannose, the arrow indicates som slightly retained proteins. __TOC__<unk>  is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. All variants of<unk>  require immunoglobulins, also known as antibodies, reacting with the proteins to be separated or characterized. The methods were developed and used extensively during the second half of the 20th century. In somewhat chronological order: Immunoelectrophoretic analysis (one-dimensional<unk>  ad modum Grabar), crossed<unk>  (two-dimensional quantitative<unk>  ad modum Clarke and Freeman or ad modum Laurell), rocket-immunoelectrophoresis (one-dimensional quantitative<unk>  ad modum Laurell), fused rocket<unk>  ad modum Svendsen and Harboe, affinity<unk>  ad modum Bøg-Hansen.
Immunoelectrophoresis
Counterimmunoelectrophoresis
Immunodiffusion
Radial immunodiffusion
Direct fluorescent antibody
Immunoassay
Direct agglutination test
Radioimmunoassay