The<unk>  can be done as either a slide or a tube test. The antigens necessary (OX2, OX19, and OXK) can be obtained commercially. Slide method 1.<unk>  by Slide method * Place 50-100µl of patient serum on the slide * Add a drop of desired antigen ( Proteus OX19 or OX2 or OXK) * Mix the suspension by rotating the slide for 1 minutes * Visible agglutination indicates the positive test . Tube method Using 0.25% phenol saline as a diluent, a series of tubes containing twofold dilutions of patient serum are made with a final volume of 1 mL. A drop of antigen suspension is added to each tube, and the mixture is incubated at 50–55 °C for 4–6 hours. A positive tube would show visible flocculation or granulation, which is accentuated when the tube is gently agitated. The titer corresponds to the most dilute tube in the series that still shows positivity. Generally, a titer of ≥1:320 is considered diagnostic.
Weil–Felix test
Hawkins–Kennedy test
Rinne test
Patrick's test
Elek's test
Bourne test
Buehler test
Draize test