3-Part Differential Cell Counter Schematic diagram of 3-part<unk> . A 3-part differential cell counter uses Coulter's Principle to find the size and volume of the cell. The sample is lysed and dissolved into an electrolyte solution in a container, which also holds a smaller container. The smaller container has 2 pumps running to and from its solution, one creating a vacuum and the other replacing the lost solution. The smaller container has a small hole (an orifice) near the bottom of the container. Coulter's principle is applied through the use of two electrodes. One electrode (the internal electrode) is within the smaller container, and the other (the external electrode) is outside of the smaller container but within the electrolyte/sample solution. As the vacuum draws the sample cells through the orifice, the cell momentarily causes electrical resistance to the current as it passes through the orifice. This resistance is recorded, measured, amplified, and processed which can then be interpreted by the computer into a histogram. The 3-part<unk>  is able to differentiate between 3 types of WBC's, neutrophils, lymphocytes, and monocytes. 5-Part Differential Cell Counter This type of<unk>  utilizes both Coulter's Principle and flow cytometry to determine the granularity, diameter, and inner complexity of the cells. Using hydrodynamic focusing, the cells are sent through an aperture one cell at a time. During this, a laser is directed at them, and the scattered light is measured at multiple angles. The absorbance is also recorded. The cell can be identified based on the intensity of the scattered light and the level of absorbance. A 5-part cell counter can differentiate all WBC types (neutrophils, lymphocytes, basophils, eosinophils, and monocytes). 5-part<unk> s are more expensive than 3-part<unk> s, but provide more in-depth information about the sample. Specific jobs, such as allergy testing, require 5-part differential analysis. However, most medical tasks can be completed with the 3-part<unk> .
Hematology analyzer
Clinical chemistry
Glucose meter
Steatocrit
Hematocrit
Blood smear
Glycated hemoglobin
Blood urea nitrogen