two-photon<unk>  over a period of 54 minutes: microglial cells of the brain responding to acute laser lesion in an Alzheimer’s disease mouse. Microglial cells of this transgenic mouse produce GFP that allows cells to be visualised (green). The ability of microglial cells (green) to extend toward a laser lesion is reduced in Alzheimer’s disease mice. β-amyloid plaques (blue) are always present in brains of Alzheimer patients .<unk>  is a form of<unk>  that allows observing biological processes in live animals (in vivo) at a high resolution that makes distinguishing between individual cells of a tissue possible. Before an animal can be used for<unk>  imaging it has to undergo a surgery involving implantation of an imaging window. For example, if researchers want to visualize liver cells of a live mouse they will implant an imaging window into mouse’s abdomen. Mice are the most common choice of animals for<unk>  but in special cases other rodents such as rats might be more suitable. Animals are always anesthetized throughout surgeries and imaging sessions.<unk>  is used in several areas of research including neurology, immunology, stem cell studies and others. This technique is particularly useful to assess a progression of a disease or an effect of a drug.
Intravital microscopy
Multiple electrode aggregometry
Diffuse optical imaging
Colorimetric capnography
Laser Doppler imaging
Iatrochemistry
Retinal vessel analysis
Photoplethysmogram