SIAM, a Novel NMR Experiment for the Determination of Homonuclear Coupling Constants.
The simultaneous acquisition of in-phase and antiphase multiplets with high sensitivity and minimum overlap (see section of 2D spectra on the right) is possible in a novel NMR experiment. Based on this method, homonuclear coupling constants such as the 3 J(HN ,Hα ) couplings in peptides and proteins can be determined quantitatively without isotope labeling.