Determination of viable mammalian cells by luminol chemiluminescence using microperoxidase.
Chemiluminescent assay for menadione-catalyzed H(2)O(2) production by mammalian cells was modified by luminol chemiluminescence with microperoxidase instead of peroxyoxalate chemiluminescence with carcinogenic fluorescent materials. Luminol can be used as a common chemiluminescent reagent for the determination of viable mammalian cells and bacteria.