[Comparative study on HPLC fingerprint of ordinary powder and ultrafine powder for Gardenia jasminoides f. longicarpa].
To establish an HPLC fingerprint of Gardenia jasminoides f. longicarpa and compare the differences between its ordinary powder and ultrafine powder. The analysis was carried out on a Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.4% phosphoric acid at the flow rate of 1.0 ml/min. The wavelength was 240 nm during 0 - 40 min and 440 nm during 40 - 80 min. HPLC fingerprint of Gardenia jasminoides f. longicarpa was established, 23 common peaks were identified,and the similarity of 10 samples was greater than 0.9. Ultrafine grinding did not change the types and number of chemical compositions, but it obviously increased the content of main chemical compositions. The HPLC fingerprint is accurate, reliable and repeatable, which can be used for quality control of Gardenia jasminoides f. longicarpa. Ultrafine grinding can stimulate the release of chemical components of Gardenia jasminoides f. longicarpa.