Rapid and efficient syntheses of phosphorylated dinucleotides.
Nine or ten-step solution phase syntheses of the dimers dpApA, dApAp, dpTpT and dTpTp in 0.1-0.5 g amounts, in overall yields of 49%, 45%, 32%, and 20% respectively, are described. The synthetic intermediates were characterized by 1H and 31 P NMR and the structures of the final products were established by hydrolysis with alkaline phosphatase and comparison of the products with authentic samples.