Transpeptidation-directed intramolecular bipartite tetracysteine display for sortase activity assay.
We developed a label-free method to measure sortase activity by using transpeptidation-directed intramolecular bipartite tetracysteine display. This assay is homogenous with a detection limit of 10.7 ng mL-1, and it can be applied to characterize Sortase A (SrtA)-targeted inhibitors, providing a new approach for anti-virulence drug discovery.