Direct ADCC lysis of O,Rh-positive (R1R2) erythrocytes by lymphocytes of individuals sensitized against antigen D.
Non-T-cells from individuals sensitized against antigen D were found to lyze O,Rh-positive erythrocytes. The lytic effect was abolished by incubation of the effector cells at 37 degrees C for 30 min, and was reconstituted by addition of anti-D antibodies. These results suggested that sensitized donors have lymphocytes armed with specific antibodies in vivo.