Terminal deoxynucleotidyl transferase activity in thymocytes of kittens.
Activity of terminal deoxynucleotidyl transferase (TdT) was measured in the thymus of 8- to 14-week-old kittens. Microscopic evidence of involution or other morphologic changes were not observed in formalin-fixed hematoxylin and eosin-stained thymic tissue sections. Thymic tissue from young kittens (8 weeks old) had low TdT activity, as determined by enzymatic catalysis or immunoassay procedures. Markedly higher TdT activity was measured in thymic tissue of older kittens (13 to 14 weeks old). Feline TdT catalyzed the polymerization of deoxynucleotides at a higher rate in Mn2+ than in Mg2+. Results of protein immunoblotting experiments indicated that the major form of feline thymic TdT is a single, high molecular weight polypeptide in 13- to 14-week-old kittens. Detection of TdT activity in the feline thymus indicated that TdT may have the same diagnostic and therapeutic value in feline oncology as in human oncology.