Effect of ionic strength on the activity of native and acetylated ribonuclease.
Acetylation of free amino groups in RNAase abolishes the stimulating effect of ionic strength on the enzyme activity. Distinct differences in the effect of ionic strength on catalytic function of native and acetylated RNAase are observed only with the natural substrate, RNA. In the presence of synthetic substrates, cyclic cytidine 2':3'-phosphate and cytidyl(3'-5')adenosine phosphate, this effect was not observed.