Effects of bovine parathyroid extract and [Asu1,7]-eel calcitonin on 35S incorporation into chick embryonic cartilage.
35S incorporation into embryonic chick pelvic cartilage was measured using the method of somatomedin bioassay and the effects thereupon of the bovine parathyroid extract (PTE) and the synthetic [Asu1,7]-eel calcitonin (CT) were examined. Bovine PTE potentiated 35S incorporation stimulated by normal human serum. [Asu1,7]-eel CT did not affect the sulfation activity in the presence of normal human serum. However, it showed a slight stimulation of 35S incorporation in the absence of normal human serum. No definite interaction on 35S incorporation between PTE and CT was observed. It is speculated from these results that PTH may be involved in the stimulation of the cartilage growth in concert with somatomedins in vivo.