Changes in grapevine leaf phenolic profiles during the day are temperature rather than irradiance driven.
Photosynthesis parameters, adaxial flavonoid index, phenolic profiles and antioxidant capacities of south-facing sun exposed grapevine leaves (Vitis vinifera, Pinot Noir cultivar) were measured hourly between 7 a.m. and 7 p.m. on a clear summer day. Changes in these parameters were statistically compared to changes in environmental conditions, including solar irradiance (photosynthetically active and UV radiations), leaf and air temperature, and relative air humidity. Epidermal UV absorbance, characterised by the flavonoid index, and total extractable phenolic contents were correlated to distinct environmental parameters. The former was positively correlated to irradiance and leaf temperature, while the latter was positively correlated to air temperature. HPLC phenolic profiling identified a positive correlation between air temperature and amounts of the dominant flavonol component, quercetin-3-O-glucuronide. The only phenolic component statistically connected to the flavonoid index was quercetin-3-O-glucoside. This correlation was positive and both parameters decreased during the day, although changes in the amount of this flavonol component showed no correlation to environmental factors. Total antioxidant capacities of leaf extracts were positively correlated to solar UV, and leaf and air temperature, but not to photosynthetically active radiation. Positive correlations of quercetin-3-O-glucoside contents with the flavonoid index, with photosynthesis and with sub-stomatal CO2 concentration suggest a special protective role of this flavonol. A short-term negative effect of solar UV-A and UV-B on photosynthetic CO2 uptake was also identified, which was unrelated to changes in stomatal conductance. A hypothesis is presented assuming UV- and photorespiration-derived hydrogen peroxide as the driver of daily changes in leaf antioxidant capacities.