The present invention generally relates to the deglycerolization of blood, and more particularly, to a system which controls the deglycerolization of blood by monitoring the segregation of erythrocytes by size.
The Armed Services Blood Program Office (ASBPO) has established a policy of maintaining pre-positioned stockpiles of frozen red blood cells, and utilizing these stockpiles in times of conflict for U.S. combat casualties. In order to implement this policy, glycerol is allowed to be absorbed by red blood cells, which then are frozen and stored. The glycerol prevents damage to the erythrocytes. Presently, the only method approved by the Food and Drug Administration (FDA) for processing thawed-frozen red blood cells uses an open, nonsterile wash system that is manually monitored and operated. This system generally requires about 11/2 to 2 hours to thaw and deglycerolize red blood cells from a cryogenic state. Because this system is not sterile, the FDA mandates that thawed-frozen red blood cells processed this way must be transfused within 24 hours or discarded. However, the time restrictions and requirement to discard the blood are not compatible with the logistics of the ASBPO policy. Therefore, a need exist for a sterile, automated method for monitoring and controlling the deglycerolization of thawed red blood cells in a more timely manner compared to the processing time of the standard method.