Technical Field
The invention generally relates to compositions and methods for reprogramming non-pluripotent cells. More particularly, the invention relates to reprogramming mononuclear cells from low volume samples of blood.
Description of Related Art
Through their ability to self-renew and differentiate into any somatic cell type, induced pluripotent stem cells (iPSCs) have remarkable, but as yet untapped, therapeutic potential. Blood provides an attractive source of somatic cells for reprogramming as it is available in relatively large volumes and is easy to collect. However, blood, like other sources of explant-derived somatic cells for reprogramming, has some limitations in therapeutic applications since it must be HLA-typed and screened for pathogens before reprogrammed cells can be administered to a subject. What is needed in the art therefore is a readily accessible source of mononuclear cells for reprogramming that has been screened for HLA compatibility, pathogens and genetic abnormalities in the donor.