Infectious disease affect multiple organs systems and are responsible for significant morbidity, mortality, and economic impact. Infectious agents most often present as a complex polymicrobial infections rather than as a single pathogens. Within the body, these polymicrobial infections cooperate with each other through mutualism changing both the type of antibiotics the organisms are susceptible to but also the level of antibiotics required to treat the infection as well as the virulence of the individual pathogens. The current gold standard is culture and sensitivity; a process in which a sample of is placed on a plate containing an agar medium for an overnight incubation allowing for individual organisms to be isolated and identified. An additional day is usually required to identify which antibiotic the individual organism is susceptible to through antibiotic susceptibility testing. The effectiveness of the this process is limited by the inherently poor sensitivity and specificity of the methodology. For example, recent studies have found that up to 25% of all urine culture results produce a false negative finding and that up to ⅔ of all uropathogens are missed by culture. This process which typically takes 48-72 hours has a significant impact on clinical practice in that clinicians are forced to treat the patient empirically without an accurate diagnosis and without proper guidance regarding the type of antibiotic that would be effective for treating the infection.
An inherent flaw in current antibiotic susceptibility testing is that it relies on testing only an individual organism rather than the entire pool of organisms simultaneously. By necessitating the isolation it adds a minimum of one additional day to the testing process. There is a need for a test that allows for the simultaneous assessment of antibiotic resistance without the need for isolation allowing for a more rapid determination of antibiotic resistance. The method described herein is a simple cost effective methods for assessing antibiotic resistance in all constituents simultaneously without the need for prior isolation.