Currently, Chinese herbal injections for treatment of cardio-cerebral vascular diseases and fundus diseases include Ixeris Sonchifolia Hance injection, which for clinical application is a brown-yellow transparent liquid derived from Ixeris Sonchifolia Hance of Compositae and extracted as intravenous injection starting from full plants. The main pharmaceutical components of the intravenous injection are flavone and adenosine. The coexistence of phyto-flavone and -adenosine in Ixeris Sonchifolia Hance has shown a remarkable complementary effect for treatment of cardio-cerebral diseases, which has already been demonstrated by pharmacodynamics study and clinical application. The pharmacological effects of Ixeris Sonchifolia Hance injection consist in its efficacies of: 1. increasing coronary artery flow, lowering cardiovascular resistance, resisting myocardial infarction, enhancing collateral circulations, reducing myocardial oxygen consumption and improving cardio-microcirculations, which are useful for the treatment of coronary heart diseases, angina pectoris, chest distress and breath shortness, and myocardial infarction; 2. reducing platelet conglomeration, increasing the activity of fibrinolysin, inhibiting thrombosis, decreasing the viscosity of blood plasma and serum, increasing the electrophoresis velocity of erythrocytes, lowering cerebro-vascular resistance, increasing cerebral blood capacity and promoting restoration of neural function, which are useful in the treatment of cerebral infarction (cerebral thrombosis); 3. improving microcirculation disorders caused by bacteria, systemic microcirculation disorders caused by polymer dextran, and fundus microcirculation, and dilating fundus artery, which are useful in the treatment of fundus diseases, such as central retinitis, optic atrophy and retinitis pigmentosa. However, Ixeris Sonchifolia Hance injections exhibit poor stability and can only stand short-term storage. The contents of flavone and adenosine have been determined by High Performance Liquid chromatography (HPLC) and derivatization method, which show that adenosine content reduces from 15.0 μg/ml prior to finishing of the aqueous injection to 6.7 μg/ml thereafter, while for flavone, from 0.25 mg/ml to 0.169 mg/ml, with a lose of 55.3% and 32.4% of the prescribed, respectively. Six months later, adenosine content reduces to 6.5 μg/ml, while flavone content is 0.133 mg/ml. This can also be verified by subjecting Ixeris Sonchifolia Hance aqueous injection wherein flavone and adenosine contents are respectively 5.07 mg/ml and 24.37 μg/ml, to ten-day accelerated stress test carried out at 80° C. in an oven, followed by content determination of both, in doing so, merely 3.16 mg/ml and 12.18 μg/ml are left for flavone and adenosine, respectively (Tab. 1). In view of the considerable lose of both active ingredients of flavone and adenosine during preparation and storage, the therapeutic effect of the injection is seriously impacted. Moreover, Ixeris Sonchifolia Hance injection exhibits a distinct change in color of solution. The absorbance measured at 400 nm using ultraviolet spectrophotometer during preparation of the aqueous injection from stock extract, ranges from 0.338 prior to sterilization to 0.423 thereafter, and further increases to 0.443 after 6 months with darkened color. It should be further noted that HPLC spectra of the injection exhibit poor similarity, that is to say, there exist major differences among HPLC spectra of different batches of aqueous injections yet formulated from the same stock extract. The stability behavior of same kind of commercial aqueous injections by random sampling (Batch No. 20000303, 20000503, 20010120) shows that: flavone contents are 0.27 mg/ml, 0.51 mg/ml and 0.30 mg/ml, while for adenosine, the contents are 1.11 μg/ml, 0.147 μg/ml and 0.00 μg/ml, respectively
In a conclusion, Ixeris Sonchifolia Hance aqueous injection is susceptible to various factors such as preparation, storage, and thus is difficult to be controlled in quality, which greatly expenses the therapeutic effect of Ixeris Sonchifolia Hance injection.