to control depth of field and amount of light what should be adjusted
Depth of field For a given subject framing and camera position, the DOF is controlled by the lens aperture diameter, which is usually specified as the f-number, the ratio of lens focal length to aperture diameter. Reducing the aperture diameter (increasing the f-number) increases the DOF because the circle of confusion is shrunk directly and indirectly by reducing the light hitting the outside of the lens which is focused to a different point than light hitting the inside of the lens due to spherical aberration caused by the construction of the lens;[7] however, it also reduces the amount of light transmitted, and increases diffraction, placing a practical limit on the extent to which DOF can be increased by reducing the aperture diameter.
Lens (anatomy) The lens is a transparent, biconvex structure in the eye that, along with the cornea, helps to refract light to be focused on the retina. The lens, by changing shape, functions to change the focal distance of the eye so that it can focus on objects at various distances, thus allowing a sharp real image of the object of interest to be formed on the retina. This adjustment of the lens is known as accommodation (see also below). Accommodation is similar to the focusing of a photographic camera via movement of its lenses. The lens is more flat on its anterior side than on its posterior side.