where does the activation of complement system take place
Complement system Most of the proteins and glycoproteins that constitute the complement system are synthesized by hepatocytes. But significant amounts are also produced by tissue macrophages, blood monocytes, and epithelial cells of the genitourinary system and gastrointestinal tract. The three pathways of activation all generate homologous variants of the protease C3-convertase. The classical complement pathway typically requires antigen-antibody complexes for activation (specific immune response), whereas the alternative pathway can be activated by spontaneous C3 hydrolysis, foreign material, pathogens, or damaged cells. The mannose-binding lectin pathway can be activated by C3 hydrolysis or antigens without the presence of antibodies (non-specific immune response). In all three pathways, C3-convertase cleaves and activates component C3, creating C3a and C3b, and causes a cascade of further cleavage and activation events. C3b binds to the surface of pathogens, leading to greater internalization by phagocytic cells by opsonization.
Two's complement In two's complement notation, a non-negative number is represented by its ordinary binary representation; in this case, the most significant bit is 0. Though, the range of numbers represented is not the same as with unsigned binary numbers. For example, an 8-bit unsigned number can represent the values 0 to 255 (11111111). However a two's complement 8-bit number can only represent positive integers from 0 to 127 (01111111), because the rest of the bit combinations with the most significant bit as '1' represent the negative integers −1 to −128.