from the lineweaver-burk plot which kinetic parameter can be determined from the y-intercept
Lineweaver–Burk plot The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents −1/Km. It also gives a quick, visual impression of the different forms of enzyme inhibition.
Runge–Kutta methods The RK4 method is a fourth-order method, meaning that the local truncation error is on the order of   O ( h 5  )  {\displaystyle O(h^{5})} , while the total accumulated error is on the order of   O ( h 4  )  {\displaystyle O(h^{4})} .