colours seen in soap bubbles are due to
Soap bubble When light shines onto a bubble it appears to change colour. Unlike those seen in a rainbow, which arise from differential refraction, the colours seen in a soap bubble arise from interference of light reflecting off the front and back surfaces of the thin soap film. Depending on the thickness of the film, different colours interfere constructively and destructively.
Nile blue It may also be used in conjunction with fluorescence microscopy to stain for the presence of polyhydroxybutyrate granules in prokaryotic or eukaryotic cells. Boiling a solution of Nile blue with sulfuric acid produces Nile red (Nile blue oxazone).