시퀀스 증폭 PCR 제품을 확인하기 위해 제조업체에서 제공한 프로토콜에 따라 BigDye Terminator v3.1 Cycling Sequencing Kit (Applied Biosystems, Foster City, 미국 CA)와 함께 ABI 373 DNA 분석기를 사용하여 직접 시퀀싱을 수행했다.
To confirm the sequence-amplified PCR product, direct sequencing was performed using an ABI 373 DNA analyzer (Applied Biosystems, Foster City, CA, USA) according to the protocol supplied by the manufacturer, along with the BigDye Terminator v3.1 Cycling Sequencing Kit (Applied Biosystems).