Ketiadaan kerangka hopanoid termetilasi pada C-2 memberikan informasi bahwa kesuburan tanah diakibatkan oleh kontribusi bakteri selain cyanobacteria seperti khizobia atau frankia.
The absence of methylated hopanoid structure in C-2 provides information that soil fertility is caused by bacteria contribution besides cyanobacteria, such as khizobia or frankia.