Melalui serangkaian tahapan reaksi kimia, luciferin dengan bantuan enzim luciferase dan beberapa zat tertentu bereaksi membentuk sejumlah zat kimia baru dengan melepaskan hampir 100% energi dalam bentuk cahaya.
Through a set of chemical reaction phase, luciferine with luciferase enzyme assisted and some certain substances  reacted to form several new chemical substances by including almost 100 percent energy on lights form