METHOD FOR IDENTIFYING AGENTS CAPABLE OF INDUCING RESPIRATORY SENSITIZATION AND ARRAY AND ANALYTICAL KITS FOR USE IN THE METHOD.The present invention relates to an in vitro method for identifying agents capable of inducing respiratory sensitization in a mammal and arrays and diagnostic kits for use in such methods. In particular, the methods include measurement of the expression of the biomarkers listed in Table 1A, Table 1B and/or Table 1C in MUTZ-3 cells exposed to a test agent.A method for identifying agents capable of inducing respiratory sensitization in a mammal comprising or consisting of the steps of: a) exposing a population of dendritic cells or a population of dendritic-like cells to a test agent; andb) measuring in the cells the expression of one or more biomarker(s) selected from the group defined in Table 1A and/or Table 1 B; wherein the expression in the cells of the one or more biomarkers measured in step (b) is indicative of the sensitizing effect of the sample to be tested.The method according to Claim 1 further comprising: c) exposing a separate population of the dendritic cells or dendritic-like cells to one or more negative control agent that is not a respiratory sensitizer in a mammal; andd) measuring in the cells the expression of the one or more biomarker(s) measured in step (b) wherein the test agent is identified as a respiratory sensitizer in the event that the presence and/or amount in the test sample of the one or more biomarker measured in step (d) differs from the presence and/or amount in the control sample of the one or more biomarker measured in step (b).The method according to Claim 1 or 2 further comprising: e) exposing a separate population of the dendritic cells or dendritic-like cells to one or more positive control agent that is a respiratory sensitizer in a mammal; andf) measuring in the cells the expression of the one or more biomarker(s) measured in step (b) wherein the test agent is identified as a respiratory sensitizer in the event that the presence and/or amount in the test sample of the one or more biomarker measured in step (f) corresponds to the presence and/or amount in the positive control sample of the one or more biomarker measured in step (b).The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of one or more biomarkers defined in Table 1A, for example, at least 2 of the biomarkers defined in Table 1A.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of OR5B21.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of SLC7A7.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of OR5B21 and SLC7A7.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of one or more of the biomarkers defined in Table 1B, for example, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , 12 or 13 of the biomarkers defined in Table 1 B.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of all of the biomarkers defined in Table 1 B.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of one or more of the biomarkers defined in Table 1C, for example, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 , 32, 33, 34, 35, 36, 37, 38, 39, 40, 41 , 42, 43, 44, 45, 46, 47, 48, 49, 50, 51 , 52, 53, 54, 55, 56, 57, 58, 59, 60, 61 , 62, 63, 64, 65, 66, 67, 68, 69, 70, 71 , 72, 73, 74, 75, 76, 77, 78, 79, 80, 81 , 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 , 92, 93, 94, 95, 96, 97, 98, 99, 100, 101 , 102, 103, 104, 105, 106, 107, 108, 109, 110, 111 , 112, 113, 114, 115, 116, 117, 118, 119, 120, 121 , 122, 123, 124, 125, 126, 127, 128, 129, 130, 131 , 132, 133, 134, 135, 136, 137, 138, 139, 140, 141 , 142, 143, 144, 145, 146, 147, 148, 149, 150, 151 , 152, 153, 154, 155, 156, 157, 158, 159, 160, 161 , 162, 163, 164, 165, 166, 167, 168, 169, 170, 171 , 172, 173, 174, 175, 176, 177, 178, 179, 180, 181 , 182, 183, 184, 185, 186, 187, 188, 189, 190, 191 , 192, 193, 194, 195, 196, 197, 198, 199, 200, 201 , 202, 203, 204, 205, 206, 207, 208, 209, 210, 211 , 212, 213, 214, 215, 216, 217, 218, 219, 220, 221 , 222, 223, 224, 225, 226, 227, 228, 229, 230, 231 , 232, 233, 234, 235, 236, 237, 238, 239, 240, 241 , 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261 , 262, 263, 264, 265, 266, 267, 268, 269, 270, 271 , 272, 273, 274, 275, 276, 277, 278, 279, 280, 281 , 282, 283, 284, 285, 286 or 287 of the biomarkers defined in Table 1C.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of all of the biomarkers defined in Table 1C.The method according to any one of the preceding claims wherein step (b) comprises or consists of measuring the expression of all of the biomarkers defined in Table 1.The method according to any one of the preceding claims wherein step (b) comprises measuring the expression of a nucleic acid molecule encoding the one or more biomarker(s).The method according to Claim 13 wherein the nucleic acid molecule is a cDNA...
호흡기 감작성을 유도할 수 있는 물질을 확인하는 방법 및 그 방법에서 사용하기 위한 어레이 및 분석 키트 본 발명은 포유동물에서 호흡기 감작성(respiratory sensitization)을 유도할 수 있는 작용제를 확인하는 인 비트로 방법 및 그러한 방법에서 사용하기 위한 어레이 및 진단 키트에 관한 것이다. 구체적으로, 상기 방법은 테스트 물질에 노출된 MUTZ-3 세포에서 표 1A, 표 1B, 및/또는 표 1C에 열거된 바이오마커의 발현의 측정을 포함한다.  포유동물에서 호흡기 감작성(respiratory sensitization)을 유도할 수 있는 물질을 확인하는 방법으로서:a) 수지상 세포의 집단, 또는 수지상-유사 세포의 집단을 테스트 물질(test agent)에 노출시키는 단계; 및b) 상기 세포에서 표 1A 및/또는 표 1B에 정의된 군으로부터 선택된 하나 이상의 바이오마커의 발현을 측정하는 단계를 포함하거나, 또는 그로 구성되고,단계 (b)에서 측정된, 상기 세포에서 상기 하나 이상의 바이오마커의 발현은 테스트되는 시료의 감작 효과를 나타내는 것인 방법.