BlyA holin family
The BlyA Holin Family (TC# 1.E.17) is a group of holin proteins that are approximately 55-70 amino acyl residues (aas) in length and exhibit one transmembrane segment (TMS). A representative list of the proteins belonging to the BlyA holin family can be found in the Transporter Classification Database. BlyAB The BlyA membrane protein and the BlyB soluble accessory protein are encoded on the conserved cp32 plasmid of Borrelia burgdorferi, which can be packaged into a bacteriophage particle. These two proteins had previously been proposed to comprise a hemolysis system, but Damman et al. (2000) provided evidence that BlyAB functions as a prophage-encoded holin system. BlyA promotes endolysin-dependent lysis of an induced lambda lysogen that is defective for the lambda holin S gene. The holin pores are generally stable and nonspecific, allowing endolysin access to the peptidoglycan. Introduction of the Borrelia burgdorferi blyAB locus into Escherichia coli produces a hemolytic phenotype that is dependent on the E. coli clyA (hlyE, sheA) gene (TC# 1.C.10.1.1). Expression of blyA in E. coli causes damage to the E. coli cell envelope and a clyA-dependent hemolytic phenotype, regardless whether blyB is present or absent. The expression of blyB in E. coli, on the other hand,