caption: A diagram where the original sample of pond water is diluted into tubes containing lactose broth (a pink broth). 10 mL of the sample is placed into each of 5 lactose broth tubes. Another 5 tubes get 1 mL each of the sample. Another 5 tubes get 0.1 mL of sample. After 24 hours of incubation at 37°C some tubes have a color change. All of the 5 tubes containing 10 mL of the sample turned yellow and show gas in the smaller inner tube. 2 of the 5 tubes that got 1 mL of the original sample turned yellow and show gas; 3 of these tubes remain pink. All of the tubes that got 0.1 mL of the original sample remain pink.
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