caption: A plan for the five-stage assembly of the M. genitalium chromosome
exif: {"Image DocumentName": "A plan for the five-stage assembly of the M. genitalium chromosome", "Image ImageDescription": "A plan for the five-stage assembly of the M. genitalium chromosome. In the first stage of assembly, four cassettes are joined to make an A-series assembly approximately 24 kb in length (assembly contained five cassettes). In the next stage, three A-assemblies are joined together to make a total of eight \u223c72-kb B-series assemblies (assembly B62-77 contained four A-series assemblies). The eighth-genome B-assemblies are taken two at a time to make quarter-genome C-series assemblies. These assemblies were all made by in vitro recombination (see Fig. 3) and cloned into E. coli using BAC vectors. Half-genome and whole-genome assemblies were made by in vivo yeast recombination. Assemblies in bold boxes were sequenced to verify their correctness. For the final molecule, the D-series half molecules were not employed. Rather, we assembled the whole molecule from the four C-series quarter molecules. A plan for the five-stage assembly of the M. genitalium chromosome.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}