Spaces:
Sleeping
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foz
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Parent(s):
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Docker container
Browse files- Dockerfile +29 -0
- app_local.py +81 -0
Dockerfile
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# https://huggingface.co/spaces/sayakpaul/demo-docker-gradio/blob/main/Dockerfile
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FROM python:3.9
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WORKDIR /code
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COPY *.py /code/
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COPY ./requirements.txt /code/requirements.txt
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RUN pip install --no-cache-dir --upgrade -r /code/requirements.txt
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# Set up a new user named "user" with user ID 1000
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RUN useradd -m -u 1000 user
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# Switch to the "user" user
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USER user
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# Set home to the user's home directory
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ENV HOME=/home/user \
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PATH=/home/user/.local/bin:$PATH
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# Set the working directory to the user's home directory
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WORKDIR $HOME/app
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# Copy the current directory contents into the container at $HOME/app setting the owner to the user
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COPY --chown=user . $HOME/app
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CMD ["python", "app_local.py"]
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app_local.py
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import gradio as gr
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from tifffile import imread
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from PIL import Image
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from path_analysis.analyse import analyse_paths
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import numpy as np
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# Function to preview the imported image
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def preview_image(file1):
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if file1:
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print('Uploading image', file1.name)
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im = imread(file1.name)
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print(im.ndim, im.shape)
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if im.ndim>2:
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return Image.fromarray(np.max(im, axis=0))
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else:
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return Image.fromarray(im)
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else:
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return None
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with gr.Blocks() as demo:
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with gr.Row():
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with gr.Column():
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# Inputs for cell ID, image, and path
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cellid_input = gr.Textbox(label="Cell ID", placeholder="Image_1")
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image_input = gr.File(label="Input foci image")
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image_preview = gr.Image(label="Max projection of foci image")
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image_input.change(fn=preview_image, inputs=image_input, outputs=image_preview)
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path_input = gr.File(label="SNT traces file")
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# Additional options wrapped in an accordion for better UI experience
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with gr.Accordion("Additional options ..."):
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sphere_radius = gr.Number(label="Trace sphere radius (um)", value=0.1984125, interactive=True)
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peak_threshold = gr.Number(label="Peak relative threshold", value=0.4, interactive=True)
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# Resolutions for xy and z axis
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with gr.Row():
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xy_res = gr.Number(label='xy-yesolution (um)', value=0.0396825, interactive=True)
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z_res = gr.Number(label='z resolution (um)', value=0.0909184, interactive=True)
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# Resolutions for xy and z axis
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threshold_type = gr.Radio(["per-trace", "per-cell"], label="Threshold-type", value="per-trace", interactive=True)
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use_corrected_positions = gr.Checkbox(label="Correct foci position measurements", value=True, interactive=True)
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screening_distance = gr.Number(label='Screening distance (voxels)', value=10, interactive=True)
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# The output column showing the result of processing
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with gr.Column():
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trace_output = gr.Image(label="Overlayed paths")
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image_output=gr.Gallery(label="Traced paths")
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plot_output=gr.Plot(label="Foci intensity traces")
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data_output=gr.DataFrame(label="Detected peak data")#, "Peak 1 pos", "Peak 1 int"])
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data_file_output=gr.File(label="Output data file (.csv)")
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def process(cellid_input, image_input, path_input, sphere_radius, peak_threshold, xy_res, z_res, threshold_type, use_corrected_positions, screening_distance):
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config = { 'sphere_radius': sphere_radius,
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'peak_threshold': peak_threshold,
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'xy_res': xy_res,
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'z_res': z_res,
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'threshold_type': threshold_type,
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'use_corrected_positions': use_corrected_positions,
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'screening_distance': screening_distance,
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}
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paths, traces, fig, extracted_peaks = analyse_paths(cellid_input, image_input.name, path_input.name, config)
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extracted_peaks.to_csv('output.csv')
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print('extracted', extracted_peaks)
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return paths, [Image.fromarray(im) for im in traces], fig, extracted_peaks, 'output.csv'
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with gr.Row():
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greet_btn = gr.Button("Process")
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greet_btn.click(fn=process, inputs=[cellid_input, image_input, path_input, sphere_radius, peak_threshold, xy_res, z_res, threshold_type, use_corrected_positions, screening_distance], outputs=[trace_output, image_output, plot_output, data_output, data_file_output], api_name="process")
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if __name__ == "__main__":
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demo.launch(server_name="0.0.0.0", share=False)
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