This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Replication, Subject name: Biochemistry
[{'content': 'Which of the following is the cofactor for Prokaryotic DNA ligase? \nSelect and explain. \nA. Tetrahydrobiopterin, B. ATP, C. NAD, D. FAD', 'role': 'user'}, {'content': 'Answer: C \nExplanation: DNA ligase : Enzyme that catalyse the formation of phosphodiester bond. During replication, the nick is sealed by DNA ligases. Use : In recombinant DNA technology as molecular glues. Cofactors : In Prokaryotes - NAD In Eukaryotes - ATP', 'role': 'assistant'}]