This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Basics of DNA, Subject name: Biochemistry
[{'content': 'Restriction endonuclease cleaves \nSelect and explain. \nA. Double stranded DNA, B. Single stranded DNA, C. Single stranded RNA, D. Polypeptide', 'role': 'user'}, {'content': "Answer: A \nExplanation: Restriction endonuclease Cleaves double stranded DNA at palindromic sites/ restriction sites 5' --AAGCTT-- 3'3' --TTCGAA-- 5' A palindromic sequence is a sequence which reads the same backwards as forwards. Found in bacteria Can create sticky ends or blunt ends Restriction endonuclease Sticky ends Blunt ends single-stranded overhangs no overhangs Example - EcoRI Example -SmaI", 'role': 'assistant'}]