This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pediatrics
[{'content': 'Beta Thalassemis is best diagnosed by? \nSelect and explain. \nA. NESTROFT Test, B. Hb A1 C estimation, C. Target cells in peripheral smear, D. Hb electrophoresis', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Hemoglobin electrophoresis performed after 6-12 months of age is usually diagnostic when levels of hemoglobin A2, hemoglobin F, or both are elevated. Thalassemia Syndrome **-Globin Genes Hb A Hb A2 Hb F Normal Homozygous 97-99% 1-3% < 1% Thalassemia major Homozygous 0 0% 4-10% 90-96% Thalassemia major Homozygous + 0-10% 4-10% 90-96% Thalassemia intermedia Homozygous + (mild) 0-30% 0-10% 6-100% Thalassemia minor Heterozygous 0 80-95% 4-8% 1-5% Heterozygous + 80-95% 4-8% 1-5% Ref: Kligman, Behrman, Jenson, Stanton (2008),Chapter 462, "Hemoglobinopathies", In the book, "Nelson\'s Textbook of Pediatrics", Volume 2, 18th Edition, New Delhi, Page 2035; Harrison\'s Internal Medicine, 16th Edition, Page 599, 608; CMDT, 2005, Page 466', 'role': 'assistant'}]