This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Medicine
[{'content': 'Nohern blot test is used for: \nSelect and explain. \nA. DNA analysis, B. RNA analysis, C. Analysis of proteins, D. Enzyme analysis', 'role': 'user'}, {'content': 'Answer: B \nExplanation: B i.e. RNA analysis - Blotting techniques separate fragments on the basis of their mass (size) by agarose or SDS- polyacrylamide gel electrophoresis (SDS-PAGE) and then transfer the pattern to nitrocellulose/nylon paper (blotting or adsorption). Then enzyme/radioactive/or fluorescence labeled probe is added complementing, hybridizing & detecting the desired fragment. Southern blotting, Nohern blotting and Western blotting techniques (Mn - "See No West") are used respectively to analyze DNA, mRNA and proteins sampleQ (sequence of protein synthesis). And the probes used in these techniques are c-DNA, cDNA and antibody respectively. - Antigen-antibody reaction is detected by ELISA (enzyme linked immune sorbent assay) and Western (immuno) blot. ELISA is sensitive and Western blot is confirmatory in AIDS diagnosisQ', 'role': 'assistant'}]