This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which of the following is an indication for restriction fragment length polymorphism? \nSelect and explain. \nA. DNA estimation, B. Synthesis of nucleic acid, C. Detecting proteins in a cell, D. Analysis of chromosome structures', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Restriction Fragment Length Polymorphism (RFLP): The human genome contains hundreds of variations in base sequences that do not affect the phenotype. The propey of the molecules to exist in more than one form is known as polymorphism. A polymorphic gene is one, in which the variant allele are common in more than 1% of the total population. The existence of two or more types of restriction fragment patterns is called restriction fragment length polymorphism. Mutation in the coding sequence (exons) lead to defective protein synthesis. But mutation in noncoding sequences (introns) have no phenotypical expression; still there will be detectable difference in the RPLF pattern. This can be used as genetic maker. Ref: Vasudevan Biochemistry, 3rd Edition, Page 381', 'role': 'assistant'}]