This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which of the following method of protein separation is not dependent on molecular size ? \nSelect and explain. \nA. Gel filtration chromatography, B. Ultracentrifugation, C. Ion-exchange chromatography, D. SDS-PAGE', 'role': 'user'}, {'content': "Answer: C \nExplanation: Ans. is 'c' i.e., Ion-exchange chromatography", 'role': 'assistant'}]