This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'PCR is a cell-free test tube method for amplifying a target sequence of DNA. Which of the following is not required for PCR? \nSelect and explain. \nA. Taq polymerase, B. d-NTP, C. Primer, D. Radiolabeled DNA probe', 'role': 'user'}, {'content': "Answer: D \nExplanation: PCR is an in vitro method for the polymerase-directed amplification of specific DNA sequences using two oligonucleotide primers. The reaction components (e.g., template, primers, Taq polymerase,2'-deoxynucleoside 5'-triphosphates, and buffer) could all be assembled and the amplification reaction carried out by simply cycling the temperatures within the reaction tube. Ref: Feng X., Lin X., Brunicardi F.C. (2010). Chapter 15. Molecular and Genomic Surgery. In F.C. Brunicardi, D.K. Andersen, T.R. Billiar, D.L. Dunn, J.G. Hunter, J.B. Matthews, R.E. Pollock (Eds), Schwaz's Principles of Surgery, 9e.", 'role': 'assistant'}]