This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: All India exam, Subject name: Pathology
[{'content': 'DNA NICK AND LABELLING (insitu) technique is used to see? \nSelect and explain. \nA. amount of total DNA in a mitochondria, B. fraction of nucleic acids, C. fraction of cells in apoptotic pathway quantified, D. fraction of cells in meotic division is quantified', 'role': 'user'}, {'content': 'Answer: C \nExplanation: DNA NICK AND LABELLING An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION. REF : robbins 10th ed', 'role': 'assistant'}]