This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which is not a step of PCR ? \nSelect and explain. \nA. Annealing, B. Extension, C. Transformation, D. Denaturation', 'role': 'user'}, {'content': "Answer: C \nExplanation: Ans. is 'c' i.e., Transformation Steps in PCRIsolation of target DNA sequence- Primer construction - Denaturation of DNA- Annealing of primers to single stranded DNA- Chain extension.", 'role': 'assistant'}]