This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Misc., Subject name: Microbiology
[{'content': 'The capsule of Cryptococcus neoformans in a CSF sample is seen by \nSelect and explain. \nA. Grams stain, B. India ink preparation, C. Giemsa stain, D. Methanamine - Silver stain', 'role': 'user'}, {'content': "Answer: B \nExplanation: (B) India ink preparation > In unstained, wet preparation of CSF with a drop of Indian ink or nigrosine, the capsule can be seen as a clear halo around the yeast cell. This is an example of negative staining.# Diagnosis of cryptococcus is established by demonstration of capsulated, budding yeast cells in the lesions and by culture.> Capsules stand out in India ink preparations.> Fungus grows readily on Sabouraud's agar forming smooth, mucoid, cream coloured colonies.", 'role': 'assistant'}]