This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which of the following assays are used to estimate amount of glycated Hemoglobin? \nSelect and explain. \nA. HPLC, B. Immunoassay, C. Affinity chromatography, D. All of the above', 'role': 'user'}, {'content': 'Answer: D \nExplanation: All the 3 assays can be used to estimate amount of glycated hemoglobin.\nNormal level of HbA1C is < 5.5%.\nHigh performance liquid chromatography employs cation exchange chromatography.', 'role': 'assistant'}]