This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Structure and function of protein, Subject name: Biochemistry
[{'content': 'Which of the following enzymes, requires Biotin as the coenzyme \nSelect and explain. \nA. Pyruvate Dehydrogenase, B. Pyruvate Kinase, C. Pyruvate Carboxylase, D. PEP Carboxy Kinase', 'role': 'user'}, {'content': 'Answer: C \nExplanation: Pyruvate enters the mitochondrion and is carboxylated to oxaloacetate by pyruvate carboxylase, an ATP-dependent reaction in which the coenzyme is the Vitamin biotin.Ref: Harper&;s Biochemistry; 30th edition; Chapter 16; The Citric Acid Cycle: The Central Pathway of Carbohydrate, Lipid & Amino Acid Metabolism', 'role': 'assistant'}]