This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Classification and metabolism of amino acids, Subject name: Biochemistry
[{'content': "Column chromatography using phenyl Sepharose beads separates proteins based on the \nSelect and explain. \nA. Affinity to ligand, B. Charge, C. Hydrophobicity, D. Stokes' radius", 'role': 'user'}, {'content': 'Answer: C \nExplanation: Hydrophobic interaction chromatography separates proteins based on their tendency to associate with a stationary phase matrix coated with hydrophobic groups(eg.phenyl Sepharose)', 'role': 'assistant'}]