This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Irreversible step (s) in glycolysis is/are: \nSelect and explain. \nA. Hexokinase, B. Phosphofructokinase, C. Pyruvate kinase, D. All of the above', 'role': 'user'}, {'content': 'Answer: D \nExplanation: All of the above', 'role': 'assistant'}]