This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
[{'content': 'Suckling mice is used for isolation of \nSelect and explain. \nA. Coxsackie virus, B. Pox, C. Herpes, D. Adenovirus', 'role': 'user'}, {'content': "Answer: A \nExplanation: It is necessary to employ suckling mice for the isolation of coxsackieviruses.\n\xa0Inoculation is usually made by intracerebral, subcutaneous and intraperitoneal\xa0route.\n\xa0Adult mice are not susceptible.\n\xa0Human diploid fibroblasts (HDF) cell line is used for cultivation of coxsackie A7,9,16; CMV; Echovirus;\xa0enteroviruses; HSV-1,2; Poliovirus: parainfluenzavirus, rhinovirus and VZV.\n\nCultivation of viruses\n‘As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium.' Three\nmethods are employed for cultivation of viruses :\n1. Animal Inoculation\n\n\xa0Earliest method\n\xa0Mice are most widely employed animals\n\xa0Other animals used are guinea pigs, rabbits and ferrets.\n\n2. Embryonated eggs\n\n\xa0Sites of inoculation are:\n\na) Chorioallantoic Membrane ------- visible pocks are produced → HSV, Vaccinia, Smallpox\nb) Amniotic Sac\nc) Allantoic cavity\n→ \xa0Influenza o Some paramyxoviruses\nd) Yolk Sac → o Some viruses o Chlamydia o Rickettsia\n3.Tissue culture\n\n\xa0Most important method for virus isolation.\n\xa0Three types of tissue culture are available\n\nI. Organ Culture\nII Explant Culture\nIII. Cell Culture - (routinely employed)\n\n\xa0Cell culture may be\n\n1. Primary cell culture: Rhesus monkey kidney, human amnion, chick embryo fibroblast, human\xa0embryonic kidney.\n2. Diploid cell strains:- Human diploid fibroblast HL-8, WI-38.\n3. Continuous cell line:- Hela, HEP-2, McCoy, Vero", 'role': 'assistant'}]