This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'The  total  body  radical  burden  can  be  estimated  by which of the following methods? \nSelect and explain. \nA. FOX  Assay, B. Estimation of Dialdehydes, C. Measurement of Pentane and Methane in exhaled air, D. All of the above', 'role': 'user'}, {'content': 'Answer: D \nExplanation: The\xa0total\xa0body\xa0radical\xa0burden\xa0can\xa0be\xa0estimated\xa0by measuring the products of lipid peroxidation:\n\nFOX (Ferrous Oxidation in Xylenol) Assay\xa0\n\tLipid peroxides can be measured by the ferrous oxidation in xylenol orange (FOX) assay. Under acidic conditions, they oxidize Fe 2+\xa0to Fe 3+, which forms a chromophore with xylenol orange.\nEstimation of Dialdehydes\n\t‒ The dialdehydes formed from lipid peroxides can be measured by reaction with thiobarbituric acid, when they form a red fluorescent adduct. The results of this assay are generally reported as total thiobarbituric acid reactive substances,TBARS.\nMeasurement of Pentane and Methane in Exhaled air\n\t‒ Peroxidation of ω-6 polyunsaturated fatty acids leads\xa0to\xa0the\xa0formation\xa0of\xa0pentane,\xa0and\xa0of-ω3 polyunsaturated fatty acids to ethane, both of which can be measured in exhaled air.\n\nReference- Review of biochemistry by Rebecca James Perumcheril Pg-412', 'role': 'assistant'}]