This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Hemoglobinopathies: Sickle cell anemia, Subject name: Pathology
[{'content': 'Eosin-5- Maleimide flow cytometry is used for diagnosis of:- \nSelect and explain. \nA. G6PD, B. Hereditary spherocytosis, C. Sickle cell anemia, D. Alpha thalassemia', 'role': 'user'}, {'content': 'Answer: B \nExplanation: - Flow cytometric analysis with EMA - labeled RBC is performed for the diagnoses of HS. - EMA is a fluorescent dye that binds to skeletal proteins of RBC. - Deficiencies of band 3, ankyrin , band 4.2 which cause HS, result in decrease of fluorescence intensity of EMA binding. - Thus, flow cytometry with EMA labeled RBCs is practical & useful test for diagnosis of HS in newborn. - Rx:- Elective Splenectomy. - Remaining options like G6PD, Sickle cell anemia, Thalassemia ; IOC is HPLC:- High performance liquid chromatography.', 'role': 'assistant'}]