This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of carbohydrate, Subject name: Biochemistry
[{'content': 'The rate-limiting enzyme in glycolysis is \nSelect and explain. \nA. Glucose 6-dehydrogenase, B. Phosphofructokinase, C. Glucokinase, D. Pyruvate kinase', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Phosphofructokinase (PFK)(step3) is the most impoant rate-limiting enzyme for glycolysis pathway. Step 3The enzyme phosphofructokinase uses another ATP molecule to transfer a phosphate group to fructose 6-phosphate to form fructose 1, 6-bisphosphate.Fructose 6-phosphate (C6H11O6P1) + phosphofructokinase + ATP - ADP + Fructose 1, 6-bisphosphate (C6H10O6P2) Ref: DM Vasudevan, 7th edition, page no: 113', 'role': 'assistant'}]