This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Blood, Subject name: Pathology
[{'content': 'The peripheral blood smear of a patient shows features of thalasemia, also presented with anaemia. Family history is also +ve. The investigation done to establish the diagnosis is : \nSelect and explain. \nA. ESR estimation, B. Blood spherocyte estimation, C. Bone marrow aspiration, D. Hb-electrophoresis', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 'd' i.e. Hb- electrophoresisElectrophoretic techniques are used for routine hemoglobin analysis. It helps in quantification and identification of various hemoglobins.As we all are aware that thalassemia is a quantitative defect in Hb characterized by lack of certain types of hemoglobin and compensatory increase in other hemoglobin, therefore electrophoresis proves to be a reliable method for screening in thalassemia.", 'role': 'assistant'}]