This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
[{'content': 'A man presented with diarrhea and lower abdominal pain. The stool is heme-positive. Investigation of choice for amoebiasis is? \nSelect and explain. \nA. ELISA, B. Colonoscopy, C. Microscopy, D. Microscopy + ELISA', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Amebiasis is infection with the parasitic intestinal protozoan Entamoeba histolytica (the "tissue-lysing ameba"). The most common extraintestinal manifestation of amebiasis is amebic liver abscess. Microscopy, often combined with enzyme-linked immunosorbent assays (ELISAs), remains the standard diagnostic approach. Ref: Stanley, S.L. (2012). Chapter 209. Amebiasis and Infection With Free-Living Amebas. In D.L. Longo, A.S. Fauci, D.L. Kasper, S.L. Hauser, J.L. Jameson, J. Loscalzo (Eds), Harrison\'s Principles of Internal Medicine, 18e.', 'role': 'assistant'}]