This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of carbohydrate, Subject name: Biochemistry
[{'content': 'Substrate level phosphorylation is catalysed by \nSelect and explain. \nA. Succinate dehydrogenase, B. Pyruvate kinase, C. Malate dehydrogenase, D. Acetyl CoA carboxylase', 'role': 'user'}, {'content': "Answer: B \nExplanation: In glycolysis, The enzyme pyruvate kinase transfers a P from phosphoenolpyruvate (PEP) to ADP to form pyruvic acid and ATP Result in step 10. Details: The final step of glycolysis conves phosphoenolpyruvate into pyruvate with the help of the enzyme pyruvate kinase. As the enzyme's name suggests, this reaction involves the transfer of a phosphate group. The phosphate group attached to the 2' carbon of the PEP is transferred to a molecule of ADP, yielding ATP. Again, since there are two molecules of PEP, here we actually generate 2 ATP molecules.Ref: DM Vasudevan, 7th edition, page no: 111", 'role': 'assistant'}]