This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Glycolysis, Subject name: Biochemistry
[{'content': 'By which of the following anticoagulants used in estimating blood glucose, glycolysis is prevented- \nSelect and explain. \nA. EDTA, B. Heparin, C. Sodium fluoride, D. Sodium citrate', 'role': 'user'}, {'content': "Answer: C \nExplanation: Ans. is 'c' i.e., Sodium Fluoride Fluoride inhibits Enolase, an important enzyme involved in Glycolysis This enzyme catalyzes the following step in glycolysis. Enolase 2 phosphoglycerate > Phosphoenol pyruvate.Inhibition of Enolase causes subsequent inhibition of Glycolysis.This property of Fluoride is utilized when blood is collected in tubes to estimate blood glucose.Fluoride inhibits glycolysis by inhibiting enolase, this will help in correct determination of the glucose level as glycolysis will not be able to affect the level of glucose.", 'role': 'assistant'}]