This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which of the following enzyme does not catalyzes irreversible step in glycolysis ? \nSelect and explain. \nA. Hexokinase, B. Phosphoglycerate kinase, C. Pyruvate kinase, D. Phosphofructokinase', 'role': 'user'}, {'content': "Answer: B \nExplanation: Ans. is 'b' i.e., Phosphoglycorate kinase Glycolysis is regulated at 3 steps which are irreversible. These reactions are catalyzed by following key enzymes :? 1) Hexokinase and glucokinase 2) Phosphofructokinase - I 3) Pyruvate kinase.", 'role': 'assistant'}]