This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Sterilization and Disinfection, Subject name: Microbiology
[{'content': 'Conditions required for autoclave is? \nSelect and explain. \nA. 121degC temperature for 20min, B. 121degC temperature for 15 min, C. 100degC temperature for 60 min, D. 100degC temperature for 90 min', 'role': 'user'}, {'content': 'Answer: B \nExplanation: ANSWER: (B) 12TC temperature for 15 minREF: Textbook of Microbiology by Ananthanarayan & Paniker 7th edition page 27, Microbiology: A Laboratory Manual, 7th ed by Cappuccin page 283Working temperature and time for different techniques of sterilization:InstrumentPrincipleControlTempPressureTimeHot air ovenDry heatClostridiumtetani160deg C 1 hourPasteurizationMoist heatNA63deg C or0 (free flowing steam)30 min (holders method) followed by rapid cooling to 13deg C or below72deg C0 (free flowing steam)15-20 sec (flash process)followed by rapid cooling to 13deg C or belowTyndallizationMoist heatNA100degC0 (free flowing steam)20 min for 3 successive days (first exposure for vegetative forms & subsequest exposures for spores)Koch steam sterilizerMoist heatNA100degC0 (free flowing steam)90 minAutoclaveMoist heat under pressureBacillusstearother-mophillus121degC15 pounds 1 /inch215 min for bacteria & spores1 hour for prions', 'role': 'assistant'}]