This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Bacteriology, Subject name: Microbiology
[{'content': 'What is the staining technique used here \nSelect and explain. \nA. Grams stain, B. Methylene blue stain, C. Albe s stain, D. Acid fast stain', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Acid-fast Pink bacilli in the blue background; M.tuberculosis Most isolates of Mycobacterium, Nocardia, and Rhodococcus species are acid-fast. Acid-fast bacteria are those that retain carbolfuchsin (basic fuchsin dissolved in a phenol-alcohol-water mixture) even when decolourized with hydrochloric acid in alcohol (due to mycolic acid in cell wall) Ref : Ananthanarayana textbook of Microbiology 9th edition Pgno : 13-14', 'role': 'assistant'}]