This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
[{'content': 'Viruses can be isolated from clinical samples by cultivation in the following except - \nSelect and explain. \nA. Tissue culture, B. Embryonated eggs, C. Animals, D. Chemically defined media', 'role': 'user'}, {'content': "Answer: D \nExplanation: Cultivation of viruses \n'As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium'.\xa0Three methods are employed for cultivation of viruses :\n1. Animal Inoculation\n\nEarliest method\nMice are most widely employed animals\nOther animals used are guinea pigs, rabbits and ferrets.\n\n2. Embryonated eggs\n\nSites of inoculation are :\n\n\nChorioallantoic Membrane -----\xa0\xa0 visible pocks are produced → HSV, Vaccinia, Smallpox\nAmniotic Sac\nAllantoic Cavity\xa0\n\n\nInfluenza\nSome paramyxoviruses\n\n\nYolk Sac\n\n\nSome viruses\nChlamydia\nRickettsia\n\n3. Tissue culture\n\nMost important method for virus isolation. \nThree types of tissue culture are available\n\n\nOrgan Culture\nExplant Culture\nCell Culture - (routinely employed)", 'role': 'assistant'}]