This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of nucleic acids, Subject name: Biochemistry
[{'content': 'RELP, True are \nSelect and explain. \nA. Endonuclease cuts DNA at nucleotide level, B. It acts at specific site, C. Only cohesive ends are produced, D. Only blunt ends are produced', 'role': 'user'}, {'content': 'Answer: B \nExplanation: The basic technique for the detecting of RFLPs involves fragmenting a sample of DNA by a restriction enzyme, which can recognize and cut DNA wherever a specific sho sequence occurs, in a process known as a restriction digest. The resulting DNA fragments are then separated by length through a process known as agarose gel electrophoresis and transferred to a membrane the Southern blot procedure. Hybridization of the membrane to a labeled DNA probe then determines the length of the fragments which are complementary to the probe. An RFLP occurs when the length of a detected fragment varies between individuals. Each fragment length is considered an allele and can be used in genetic analysis.RFLP analysis may be subdivided into single- (SLP) and multi-locus probe (MLP) paradigms. Usually, the SLP method is preferred over MLP because it is more sensitive, easier to interpret and capable of analyzing mixed-DNA samples. Moreover, data can be generated even when the DNA is degraded (e.g. when it is found in bone remains.)Ref: https://en.wikipedia.org/wiki/Restriction_fragment_length_polymorphism', 'role': 'assistant'}]