This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of nucleic acids, Subject name: Biochemistry
[{'content': 'Mutation that completely disrupts the function of the gene used in which of the following techniques? \nSelect and explain. \nA. Knock out, B. Non sense mutation, C. Restriction fragment length polymorphism, D. Targetted gene disruption', 'role': 'user'}, {'content': 'Answer: A \nExplanation: A gene knockout (abbretion: KO) is a genetic technique in which one of an organism&;s genes is made inoperative ("knocked out" of the organism). Also known as knockout organisms or simply knockouts, they are used in learning about a gene that has been sequenced, but which has an unknown or incompletely known function.', 'role': 'assistant'}]