This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Proteins are separated on the basis of charge in ? \nSelect and explain. \nA. SDS-PAGE, B. Ultracentrifugation, C. Affinity chromatography, D. HPLC', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 'd' i.e., HPLC", 'role': 'assistant'}]