This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Introduction, Subject name: Pathology
[{'content': 'Karyotyping is done in which phase of cell cycle? \nSelect and explain. \nA. Anaphase, B. Metaphase, C. Telophase, D. S phase', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Karyotyping The study of chromosomes Procedure to examine chromosomes is to arrest dividing cells in metaphase with mitotic spindle inhibitors (e.g., N-diacetyl-N-methylcolchicine ) and then to stain (Giemsa stain) the chromosomes. In a metaphase spread, the individual chromosomes take the form of two chromatids connected at the centromere.', 'role': 'assistant'}]