This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Structure & Function of Proteins), Subject name: Biochemistry
[{'content': 'Proteins are separated on the basis of charge in - \nSelect and explain. \nA. SDS-PAGE, B. Ultracentrifugation, C. Affinity chromatography, D. HPLC', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 'd' i.e., HPLC Property of protein usedMethodProtein solubility'Salting out' or salt extraction (precipitation of protein by ammonium sulfate)Molecular sizeo Gel filtration chromatography (molecular-sieve chromatography or size exclusion chromatography)o Ultracentrifugationo SDS-PAGE (Sodium dodecyi sulphate-polyacrylamide gel electrophoresis)o DialysisMolecular charge (Ionic charge)o Ion exchange chromatographyo High performance liquid chromatography (HPLC)o Electrophoresis (simple on cellulose acetate starch gel)o Isoelectric focusing (a variant of electrophoresis)Molecular charge and molecular weightPAGE : polyacrylamide gel electrophoresisAffinity bindingAffinity' chromatographyHydrophobicityHydrophobic interaction chromatography", 'role': 'assistant'}]