This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Carbohydrates, Subject name: Biochemistry
[{'content': 'The reaction catalyzed by phosphofructokinase: \nSelect and explain. \nA. Is activated by high concentrations of ATP and citrate, B. Uses fructose-l-phosphate as substrate, C. Is the rate-limiting reaction of glycolytic Pathway?, D. Is inhibited by fructose 2, 6-bisphosphate', 'role': 'user'}, {'content': 'Answer: C \nExplanation: Ans. C. Is the rate-limiting reaction of glycolytic PathwayPhosphofructokinase is the key enzyme of glycolysis. It phosphorylates Fructose-6- phosphate to form Fructose 1,6- bisphosphate. It is inhibited by ATP and Citrate ions and allosterically modified by AMP. PFK has two activities. PFK1 forms Fructose 1,6- bisphosphate and PFK2 forms Fructose 2,6-bisphosphate. The former reaction is irreversible and the latter reversible. Fructose 2,6-bisphosphate depicts the energy status of the body.', 'role': 'assistant'}]