This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Arboviruses, Subject name: Microbiology
[{'content': 'Most sensitive diagnostic test for dengue is: \nSelect and explain. \nA. IgM ELISA, B. Complement fixation test, C. Reverse transcriptase PCR, D. Electron microscopy', 'role': 'user'}, {'content': "Answer: C \nExplanation: Ans. is 'c' i.e., (RT - PCR)(Ref: Ananthanarayan, 9th/e, p. 523 and 7th/e, p. 137; Harper, 27th/e, p. 604)* Reverse transcriptase PCR (RT - PCR) based methods are available for rapid identification and serotyping of dengue virus in acute phase serum, roughly during the period of fever. Isolation of the virus is difficult.* Neutralizing and Hemagglutination inhibiting antibodies appear within a week after the onset of dengue fever. Analysis of paired acute and convalescent sera to show a significant rise in antibody titer is the most reliable evidence of an active dengue infection.", 'role': 'assistant'}]