This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Unknown
[{'content': 'Antigen-antibody reaction is detected by (PGI Nov 2009) \nSelect and explain. \nA. ELISA, B. Southern blot, C. Northern blot, D. Western blot', 'role': 'user'}, {'content': 'Answer: A \nExplanation: Ans: A (ELISA) & D (Western blot) "In western blots (also called immunoblots) protein molecules in the sample are separated by electrophoresis & blotted (transferred) to a membrane. The probe is a labeled antibody, which produces a band at the location of its antigen"- Lippincott\'s Biochemistry 4th/484Table (Lippincott\'s Biochemistry): Techniques used to analyze DNA, RNA & proteinsTechniqueSampleAnalysedGelUsedPurposeSouthern blotDNAQYesDetect DNA changesNorthern blotRNAYesMeasures mRNA amounts & sizeWestern blotproteinYesMeasures protein amountsASODNAQNoDetects DNA mutations\'\'MicroarrayRNA or cDNANoMeasures many mRNA levels at onceELISAproteins or antibodiesNoDetects proteins (anti- genes or antibodies^ProteomicsProteinsQYesMeasures abundance, distribution, post-translational modifications, functions & interactions of cellular proteinsASO= Allele-specific oligonucleotides', 'role': 'assistant'}]