This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which test uses oligomer with single base pair substitution as a primer - \nSelect and explain. \nA. PCR, B. RFLP, C. Error coded mutation analysis with PCR, D. Site directed mutagenesis', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Site-directed mutagenesis or oligonucleotide-directed mutagenesis uses oligomer with single base pair substitution as a primer to create a specific DNA sequence change (in gene), thus producing modified proteins with a change in primary structure.', 'role': 'assistant'}]