This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Technique (s) used to detect Gene Mutation is/are \nSelect and explain. \nA. #NAME?, B. Denaturing gradient gel electrophoresis, C. DNA sequencing and Restriction fragment polymorphism (RFLP) both, D. All', 'role': 'user'}, {'content': 'Answer: D \nExplanation: ALL i.e. (-PCR, Denaturing gradient gel electrophoresis, DNA sequencing, Restriction fragment polymorphism (RFLP), Single-strand conformational polymorphism', 'role': 'assistant'}]