This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Bio-Chemistry), Subject name: Biochemistry
[{'content': 'In estimation of bilirubin, the solution is examined at \nSelect and explain. \nA. 540 nm, B. 450 nm, C. 480 nm, D. 580 nm', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Ans: b (450nm) Ref: Vasudevan 4th ed/p.480The characteristic absorption maximums of some importantsubstances are:- Protein, peptide linkage- 220 nmProtein, tryptophan units- 280 nmNucleic acids- 260 nmDeoxy haemoglobin- 565 nmBilirubin- 450 nmNADH- 340 nmFAD- 450 nmFADH2- 570 nmPorphyrin- 400 nm', 'role': 'assistant'}]