This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: vitamins, Subject name: Biochemistry
[{'content': 'During the formation of hydroxyproline and hydroxylysine, the essential factors required is/are \nSelect and explain. \nA. Pyridxal phosphate, B. Ascorbic acid, C. Thiamine pyrophosphate, D. Methylcobalamine', 'role': 'user'}, {'content': 'Answer: B \nExplanation: In Collagen Synthesis: Vitamin C is required for the post-translational modification, Hydroxylation of lysine and Proline Vitamin C plays the role of a coenzyme The hydroxylation reaction is catalysed by lysyl hydroxylase (for lysine) and prolyl hydroxylase (for proline) this reaction is dependent on vitamin C(ascorbic acid), molecular oxygen and alpha-ketoglutarate Hydroxyproline and hydroxylysine are essential for the collagen cross-linking and the strength of the fibre Ref: Satyanarayana 4th ed page 132', 'role': 'assistant'}]