This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Parasitology Pa 1 (Protozoology), Subject name: Microbiology
[{'content': 'The pathogenicity of E. histolytica is indicated by - \nSelect and explain. \nA. Zymodeme pattern, B. Size, C. Nuclear pattern, D. ELISA test', 'role': 'user'}, {'content': 'Answer: A \nExplanation: Option 1, 2, 3 Zymodeme (isoenzyme) analysis: - E. histolytica possesses several isoenzymes like mailc enzyme, hexokinase, isomerase and phosphoglucomutase and these isoenzymes contribute in pathogenesis. When these isoenzymes are subjected to electrophoresis based on electrophoretic pattern (Zymodeme pattern) and mobility of these isoenzymes, Entamoeba can be diagnosed and speciated. Option 4 Amebic antigen in serum indicates recent and active infection. ELISA is done for lectin antigen Amebic antibody: Serum antibodies appear only in the later stages of intestinal amebiasis, e.g. ELISA, IFA, indirect hemagglutination test (IHA).', 'role': 'assistant'}]