This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: general microbiology, Subject name: Microbiology
[{'content': 'Disinfection of sputum is done by? \nSelect and explain. \nA. Boiling, B. Autoclaving, C. Sunlight, D. Burning', 'role': 'user'}, {'content': "Answer: A \nExplanation: Boiling: Vegetative bacteria killed almost immediately at 90-100 degree Celsius but sporing bacteria requires prolonged periods of boiling. Boiling is not recommended for sterilizing of instruments used for surgical procedure and should be regarded only as a means of disinfectant. Nothing sho of autoclaving at high pressure can destroy spore and ensure sterilization. Hard water should not be used. Sterilization may be promoted by the addition of 2% sodium bicarbonate to water. in the case where boiling is considered adequate, The material should be immersed in the water and boiled for 10-30 minutes. The lid of sterilizer should not be opened during the period ref:Ananthanarayan and Panicker's Textbook ofMicrobiology 8thEdition pg no:32-33", 'role': 'assistant'}]