This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': 'CRISPR/Cas9 mediated gene editing involves which of the following DNA repair mechanisms? \nSelect and explain. \nA. Base Excision Repair, B. Nucleotide Excision Repair, C. Non-Homologous End Joining, D. Mismatch Repair', 'role': 'user'}, {'content': 'Answer: C \nExplanation: CRISPR The CRISPR system represents a form of acquired immunity to infection by bacteriophages, which complements the system of restriction endonucleases and methylases. CRISPR uses RNA-based targeting to bring the Cas9 nuclease to foreign (or any complementary)DNA. Within bacteria this CRISPR-RNA-Cas9 complex degrades and inactivates targeted DNA. Use- gene deletion,gene editing and even modulation of gene transcription. CRISPR mediated gene therapy works on the basis of both homologous and non-homologous end joining DNA repair mechanism', 'role': 'assistant'}]