This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General anatomy, Subject name: Anatomy
[{'content': 'A chromatographic technique which separates one protein from another based on the difference in their size is \nSelect and explain. \nA. Size exclusion chromatography, B. Ion-exchange chromatography, C. Hydrophobicity, D. Affinity chromatography', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Chromatographic techniques separate one protein from another based on the difference in their size (size exclusion chromatography), charge (ion-exchangechromatography), hydrophobicity (hydrophobic interaction chromatography), or ability to bind a specific ligand (affinity chromatography).Ref: Harper&;s Biochemistry; 30th edition; Chapter 4; Proteins: Determination of Primary Structure', 'role': 'assistant'}]