This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Michaelis Menten Hypothesis states that \nSelect and explain. \nA. Rate of enzymatic reaction is independent of substrate concentration, B. Rate of non enzymatic reaction is proportional to substrate concentration, C. Km is the enzyme substrate complex association constant, D. Enzyme substrate complex formation is essential in enzymatic reaction.', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Michaelis-Menten Theory (Enzyme-Substrate Complex Theory) \nEnzyme combines with a substrate to form a transient Enzyme-Substrate Complex which immediately break into Enzyme and products.', 'role': 'assistant'}]