This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Structure and function of protein, Subject name: Biochemistry
[{'content': 'Which of the following enzymes is used in Recombinant DNA research for Homopolymer tailing \nSelect and explain. \nA. Reverse transcriptase, B. S1 Transferase, C. Polynucleotide kinase, D. Terminal transferase', 'role': 'user'}, {'content': "Answer: D \nExplanation: Terminaldeoxynucleotidyltransferase catalyzes the addition of deoxynucleotides to the 3' termini of DNA. ... By use of a tailing method, which adds homopolymerdeoxynucleotide tails to denatured DNA, any double-stranded DNA fragment can be joined to a cloning vehicle Ref biochemistry satyanarayana 4e p255", 'role': 'assistant'}]