This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Beta thalasemmia, Subject name: Pathology
[{'content': 'Which of the following is best to confirm diagnosis of thalassemia trait in a lady with positive family history of thalassemia? \nSelect and explain. \nA. Elevated HbA2, B. Reticulocytosis, C. NESTROFT, D. Peripheral smear', 'role': 'user'}, {'content': 'Answer: A \nExplanation: To Confirm diagnosis of Thalassemia trait, following can be seen HPLC of Hb Increased HbA2 levels (>3.5%) NESTROFT is useful in screening this disease.', 'role': 'assistant'}]