This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Intact peptide bond is necessary for which test: \nSelect and explain. \nA. UV diffraction, B. Ninhydrin, C. Diazo reaction, D. All', 'role': 'user'}, {'content': 'Answer: B \nExplanation: B i.e. NinhydrinAmino acids can be detected and quantified by reaction with ninhydrin after seperation of amino acid from mixtures. The test requires intact peptide bondsQ.', 'role': 'assistant'}]