This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: DNA Replication , Subject name: Biochemistry
[{'content': 'Unwinding of DNA during replication is done by- \nSelect and explain. \nA. Ligase, B. Helicase, C. Polymerase, D. Prinase', 'role': 'user'}, {'content': "Answer: B \nExplanation: Ans. is 'b' i.e., HelicaseProteins involved in replicationProteinFunctionHelicaseCauses unwinding of DNA using ATPSSB (Single strand binding protein)Keeps two strands seprate, prevents renneiling of two strandsTopisomerase I and IIThey have both nuclease (strand cutting) and ligase activity, hence they relieve torsinal strain that results from helicase induced unwindingDN A pnmaseInitiates synthesis of RNA primerDNA polymeraseDeoxyribonucleotide polymerizationDNA Jigase/nick sealingSeals the single stranded nick between nascent chaind and okazaki fragments", 'role': 'assistant'}]