This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Which of the following is true for phosphofructokinase \nSelect and explain. \nA. It uses fructose 1, 6 bisphosphate as substrate, B. It generates ATP, C. It catalyses an irreversible reaction in glycolysis, D. It is activated by increased levels of ATP and citrate', 'role': 'user'}, {'content': 'Answer: C \nExplanation: Phosphofructokinase catalyses the first committed step of glycolysis. It is the rate limiting enzyme of glycolysis. The reaction is irreversible.', 'role': 'assistant'}]