This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Parasitology Pa 1 (Protozoology), Subject name: Microbiology
[{'content': 'Selective medium for Naegleria fowleri - \nSelect and explain. \nA. Nutrient agar rich with E. coli, B. NNN media, C. Non Nutrient agar with E. coli, D. Diamond media', 'role': 'user'}, {'content': "Answer: C \nExplanation: Option 2 NNN ( Novy-MacNeal-Nicolle) Media is used for culture of Leishmania and Trypanosoma. Option 3 CSF sample can be cultivated on nonnutrient agar (Page's saline and 1.5% agar), lawn cultured with E. coli. Naegleria feeds on bacteria and crawls over the lawn culture of E. coli to produce trails. Option 4 Diamond media (Axenic culture): it is used for isolation of Trichomonas and Entamoeba histolytica.", 'role': 'assistant'}]