This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Cellular Pathology, Subject name: Pathology
[{'content': 'Best method for HbA1c estimation is? \nSelect and explain. \nA. Affinity chromatography, B. Ion exchange chromatography, C. Electrophoresis, D. HPLC', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Ans. (d) HPLCFour basic types of methods are used most commonly to measure HbA1c: immunoassay, ion-exchange high- performance liquid chromatography (HPLC), boronate affinity HPLC, and enzymatic assays. The gold standard is HPLC method', 'role': 'assistant'}]