This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: general microbiology, Subject name: Microbiology
[{'content': 'Stain is not taken by capsule in gram negative organisms becasue the capsule consist of - \nSelect and explain. \nA. Polysaccharides, B. Lipopolysaccharides, C. Lipids, D. Protein', 'role': 'user'}, {'content': "Answer: A \nExplanation: Slime has a little affinity for basic dyes and is not visible in Gram stained smears . Special capsule staining techniques are available usually employing copper salts as mordants . Capsules may be readily demonstrated by negative staining in wet films with India ink , when they are seen as clear halos around the bacteria aganist a black background Methods used for demonstration of Capsule: 1.Indian ink(Negative staining) 2.Serological Methods 3.special capsular staining Ref :Ananthanarayan & paniker's Textbook of microbiology 9th edition pg no 18,C.p.Baveja pg no 17", 'role': 'assistant'}]