This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Basics of amino acids, Subject name: Biochemistry
[{'content': 'Detection of proteins by the following methods does not affect its function \nSelect and explain. \nA. Detecting with Coomassie blue dye after electrophoresis by SDS-PAGE, B. Detecting with heat coagulation, C. Detecting with 2-D electrophoresis, D. Detecting with UV light at 280 nm', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Option analysis: * Sodium dodecyl sulfate is a protein denaturing agent - option a is excluded Heat coagulation result in loss of higher level of structures other than primary structure - option b is excluded 2-D electrophoresis is SDS-PAGE in one dimension and isoelectric focusing in other dimension - option c is excluded Hence the answer is UV light. In this, protein solution is exposed to UV light and amino acids in protein absorb UV light which can be measured using a spectrophotometer. This technique does not affect the structure of proteins hence does not affect its function.', 'role': 'assistant'}]