This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General, Subject name: Microbiology
[{'content': 'Dark fieid microscopy used in - \nSelect and explain. \nA. Vibrio, B. Syphilis, C. TB, D. Brucellosis', 'role': 'user'}, {'content': "Answer: B \nExplanation: Ans. is 'b' i.e., Syphilis o Dark field microscopy is used for spirochetes (for example T pallidum which causes syphilis).Dark ground/ Dark field microscopeo Very slender organisms such as spirochetes and filamentous organs such as flagella are not visible under ordinary illumination.o The contrast in dark field microscopy gives an illusion of increased resolution so that these structures can be seen under the dark field mcroscope.o In dark field microscopy reflected light is used instead of the transmitted light used in the ordinary microscope,o Dark ground (dark field) microscopy is used for :FlagellaSpirochetesRememberComponentMethod of detectionFlagellaFimbriaeSporesCapsuleDark ground microscopeHemagglutinationAcid fast staining by Ziehl-Neelsen techniquey Negative staining by India inky Quellung reaction", 'role': 'assistant'}]