This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: DNA Replication , Subject name: Biochemistry
[{'content': 'Action of topoisomerase III - \nSelect and explain. \nA. Remove positive supercoil, B. Remove negative supercoil, C. Form negative supercoid, D. Single strand break', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 4d' i.e., Single strand breako Topoisomerase I - Removes negative supercoiling.o Topoisomerase II - Relaxes positive supercoils and forms negative supercoiling by condensation, of chromosome,o Topoisomerase III - Can introduce single strand breaks during recombination that are required for DNA to be exchanged by adjacent chromosome.o Topoisomerase IV- Acts to disentangle the replicated intertwined DNA strands by making double-strand breaks that allow one duplex to pass through the other.", 'role': 'assistant'}]