This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: general microbiology, Subject name: Microbiology
[{'content': 'Not used in grams staining? \nSelect and explain. \nA. Methylene blue, B. Crystla violet, C. Iodine, D. Safranin', 'role': 'user'}, {'content': "Answer: A \nExplanation: GRAM STAINING 1)primary staining with a pararosaniline dye such as crystal violet, methyl violet, or gentian violet 2)application of a dilute solution of iodine 3) decolorization with an inorganic solvent such as ethanol, acetone, or aniline 4)counterstaining with a dye of contrasting color such as carbon fuchsin, safranine or neutral red REF:Ananthanarayan & Panicker's Textbook of Microbiology 8th Edition pg no: 15", 'role': 'assistant'}]