This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Structure and function of protein, Subject name: Biochemistry
[{'content': 'Which of the following technique for protein separation is based on molecular size? \nSelect and explain. \nA. Ion exchange chromatography, B. Gel filtration chromatography, C. Affinity chromatography, D. Hydrophobic interaction chromatography', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Gel filtration chromatography is known as molecular sieving.Hyrophilic cross linked gels like acrylamide, agarose ,and dextran(sephadex)are used for seperation of molecules based on their size.Sephadex is widelyb used and the range of seperation is based on the pore size.The gel is porus in nature.The small paicls enter ino the gel and reenter into the another paiclebut the large immunoglobulins cannot enter the pores and sidetrack the gel paicles:so they move rapidly.In sho larger molecules will come out first; while smaller molecules retained in the column. REFERENCE ; DM VASUDEVAN Textbook SEVENTH EDITION Page no: 450', 'role': 'assistant'}]