This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Medicine
[{'content': 'Which test uses oligomer with single base pair substitution as primer \nSelect and explain. \nA. PCR, B. RFLP, C. Error coded mutation analysis with PCR, D. Site directed mutagenesis', 'role': 'user'}, {'content': 'Answer: D \nExplanation: D i.e. Site directed mutagenesis Site directed mutagenesis and oligonucleotide directed mutagenesis uses oligomer with single base pair substitution as primerQ to create a specific DNA sequence change (in gene), thus producing modified proteins with changes in primary structure of protein. This helps researchers to examine the effect of these changes on 3-D structure, folding & activity of protein.', 'role': 'assistant'}]