This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General Microbiology (Sterilization and Bacterial Genetics), Subject name: Microbiology
[{'content': 'Most effective method of sterilization by heat is \nSelect and explain. \nA. Hot air, B. Boiling, C. Steam, D. Steam under pressure', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Most effective method of sterilization by heat is by using moist heat (steam) above atmospheric pressure Autoclave is a classical example. Principle of autoclave- when water boils under pressure, instead of boiling at 100 degrees, it boils at a higher temperature. Hence the steam generated is at higher temperature.', 'role': 'assistant'}]