This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Structure & Function of Proteins), Subject name: Biochemistry
[{'content': 'Ion exchange chromatography is based on - \nSelect and explain. \nA. Size, B. Charge, C. Solubility, D. Polarity', 'role': 'user'}, {'content': "Answer: B \nExplanation: Ans. is 'b' i.e.. Charge Property of protein usedMethodProtein solubility'Salting out' or salt extraction (precipitation of protein by ammonium sulfate)Molecular sizeo Gel filtration chromatography (molecular-sieve chromatography or size exclusion chromatography)o Ultracentrifugationo SDS-PAGE (Sodium dodecvl sulphate-polyacry lamide gel electrophoresis)o DialysisMolecular charge (Ionic charge)o Ion exchange chromatographyo High performance liquid chromatography (HPLC)o Electrophoresis (simple on cellulose acetate starch gel)o Isoelectric focusing (a variant of electrophoresis)Molecular charge and molecular w eightPAGE : polyacrylamide gel electrophoresisAffinity bindingAffinity chromatographyHvdnophobidty'Hydrophobic interaction chromatography", 'role': 'assistant'}]