This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: C. Mycobacteria, Subject name: Skin
[{'content': "How many bacilli should be there in the tissue for slit skin smear to be positive for Hansen's disease? \nSelect and explain. \nA. 10 baccili / gram of tissue, B. 102 baccili / gram of tissue, C. 103 baccili i gram of tissue, D. 104 baccili / gram of tissue", 'role': 'user'}, {'content': 'Answer: D \nExplanation: Ref: Rook s Textbook of DermatologyExplanation:At least 1114 bacilli should be present per gram tissue for 1+ bacteriological index in slit skin smear,Maximum number of bacilli in a highly bacillated lepromatous leprosy patient is 1012.Average density of bacilli in lepromatous leprosy is 2.5 billion per 1 cm3 tissue.', 'role': 'assistant'}]