This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Carbohydrates, Subject name: Biochemistry
[{'content': 'In glycogen metabolism, amylo 1-6-glycosidase acts on glycogen to produce? \nSelect and explain. \nA. Glucose-l-phosphate, B. Glucose-6-phosphate, C. Maltose, D. Glucose', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Ans. d (Glucose); (Ref Harper, Biochemistry, 26th ed., 145; Satyanaryan, Biochemistry, 2nd ed., 273)GLYCOGEN# Glycogen contains alpha-1-4 and alpha-1-6 links.# Degradation of glycogen to glucose involves 4 enzyme reactions:- Phosphorylase, which hydrolyses alpha-1-4 bond- Amylo 1-6-glycosidase, a debranching enzyme that removes alpha-1-6 bond to produce free glucose in muscle.- Phosphoglucomutase, which converts glucose-1-phosphate to glucose-6-phosphate.- Glucose-1-phosphatase, which converts glucose-6-phosphate to glucose.# Thus, amylo 1-6-glycosidase (debranching enzyme) breaks alpha-1-6 bond at branch with single glucose residue and releases free glucose.', 'role': 'assistant'}]