This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Blood, Subject name: Pathology
[{'content': 'Eosin-5-Maleamide flow cytometry is used for diagnosis of: \nSelect and explain. \nA. G6PD, B. Hereditary spherocytosis, C. Sickle cell anemia, D. Alpha thalassemia', 'role': 'user'}, {'content': "Answer: B \nExplanation: Ans: (b) Hereditary spherocytosisRef: Wintrobe's Hematology, compiledHereditary SpherocytosisAD inheritanceMost common mutation - Ankyrin*Most severe mutation Spectrin*The spherocytes of HS have 2 defects:Reduced deformabilityReduced membrane plasticityPeripheral smearSpherocytesHowell jolly bodies - post splenectomyHemolysis - ExtravascularDiagnosisPeripheral smearOsmotic fragility test - ScreeningGold standard - Flow cytometry - EMA (Eosin-5-Maleamide) Flowcytometry EMA FlowcytometryA flowcytometric test based on eosin-5-maleamide (EMA) which binds to band 3 of red cell membrane is a sensitive and specific test for diagnosis of HS.The mean fluorescence intensity of EMA tagged red cells is lower in HS (MCF<0.80).EMA fluorescence is normal or increased in AIHA (DD for spherocytosis).", 'role': 'assistant'}]