This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of carbohydrate, Subject name: Biochemistry
[{'content': 'Which of the following is required to bring about gluconeogenesis from pyruvate? \nSelect and explain. \nA. Pyruvate dehydrogenase, B. Biotin, C. Alpha ketoglutarate dehydrogenase, D. Fructose-6 phosphate', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalysed by a mitochondrial enzyme, pyruvate carboxylase (Fig. 9.24). It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, 7th edition, page no: 118', 'role': 'assistant'}]