This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Medicine
[{'content': 'Mutation that completely disrupt the function of the gene used in which of the following technique: \nSelect and explain. \nA. Knock-out, B. Non-sense mutation, C. Restrictin fragment length polymorphism, D. None', 'role': 'user'}, {'content': 'Answer: A \nExplanation: A i.e. Knock-out', 'role': 'assistant'}]