This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': "Which of the following methods is most suitable for isolating gene of long DNA molecules \nSelect and explain. \nA. Sanger's sequencing, B. Southern blotting RFCP, C. RFCP, D. Chromosome walking", 'role': 'user'}, {'content': 'Answer: D \nExplanation: Chromosome walking is the best method to isolate gene from long segment of DNA.', 'role': 'assistant'}]