This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Mycology, Subject name: Microbiology
[{'content': 'Test used to diagnosis Pneumocystis Jirovecci - \nSelect and explain. \nA. Methenamine silver, B. PCR, C. Immunofluorescence, D. All of the above', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 'd' i.e., All of the above Diagnosis of Pneumocystis Tirovecci* Best specimen is bronchoscopy with BAL (bronchoalveolar lavage) fluid. Sputum can also be used.* Following are methods for diagnosisi) Histopathologic staining for cysts and other stages: It is most commonly used method for diagnosis. Methenamine silver, toluidine blue and crystal violet stain the cyst wall. Wright Geimsa stains all development stages.ii) Immunofluorescence with monoclonal antibodies.iii) PCR: It is most sensitive.", 'role': 'assistant'}]