This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Virology, Subject name: Microbiology
[{'content': 'Viruses can be grown using all of the following except: \nSelect and explain. \nA. Tissue culture, B. Embryonated eggs, C. Animals, D. Enriched media', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. D. Enriched mediaViruses are strict/obligate cellular parasites, as they lack necessary enzymes for nucleic acid and protein synthesis. They are dependent on host cells for this, hence can't grow in cell free (artificial) culture medium.Methods for virus isolation/culturea. Animalb. Embryonated eggs (CAM, Amnion, Allantoic, Yolk sac)c. Tissue Culture", 'role': 'assistant'}]