This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Structure & Function of Proteins), Subject name: Biochemistry
[{'content': 'Protein separation based on moleular size - \nSelect and explain. \nA. HPLC, B. Gel filtration chromatography, C. Salting out, D. Affinity chromatography', 'role': 'user'}, {'content': "Answer: B \nExplanation: Ans. is 'b' i.e., Gel filtration chromatographyProperty of protein usedMethodProtein solubility'Salting out' or salt extraction (precipitation of protein by ammonium sulfate)Molecular sizeo Gel filtration chromatography (molecular-sieve chromatography or size exclusion chromatography)o Ultracentrifugationo SDS-PAGE (Sodium dodecyl sulphate-polyacrylamide gel electrophoresis)o DialysisMolecular charge (Ionic charge)o Ion exchange chromatographyo High performance liquid chromatography (HPLC)o Electrophoresis (simple on cellulose acetate starch gel)o Isoelectric focusing (a variant of electrophoresis)Molecular charge and molecular weightPAGE : polyacrylamide gel electrophoresisAffinity bindingAffinity chromatographyHydrophobicityHydrophobic interaction chromatography", 'role': 'assistant'}]