This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General Microbiology (Sterilization and Bacterial Genetics), Subject name: Microbiology
[{'content': 'Tyndallization is a method of sterilization using; \nSelect and explain. \nA. Moist heat above 100 C, B. Moist heat below 100 C, C. Dry heat above 100 C, D. Moist heat at 100 C', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Tyndallization or intermittent sterilization uses moist heat at 100 C for 20 minutes for 3 consecutive days. It is carried out in a Koch and Arnold steam sterilizer. Media which contain any ingredient/component not able to tolerate the temperature of an autoclave are sterilized by tyndallization. Eg. TCBS, XLD, DCA, sugar solutions for fermentation tests and gelatin containing media.', 'role': 'assistant'}]