This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': 'SYBR Green Dye is used for: \nSelect and explain. \nA. HPLC, B. Immuno-fluorescence, C. PCR, D. ELISA', 'role': 'user'}, {'content': 'Answer: C \nExplanation: Dyes used in real time PCR SYBR based detection TaqMan based detection Uses SYBR Green dye ds-DNA binding dye Not sequence specific Uses a fluorogenic probe specific to target gene It accumulates during PCR. Real Time PCR / Quantitative Fluorescence PCR (QF-PCR) / q PCR: Products are detected by fluorescence during (NOT after) the PCR reaction (in real time), not end time PCR.', 'role': 'assistant'}]