This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Action of topoisomerase III ? \nSelect and explain. \nA. Remove positive supercoil, B. Remove negative supercoil, C. Form negative supercoid, D. Single strand break', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 'd' i.e., Single strand breakTopoisomerase I Removes negative supercoiling.Topoisomerase II Relaxes positive supercoils and forms negative supercoiling by condensation. of chromosome.Topoisomerase III Can introduce single strand breaks during recombination that are required for DNA to be exchanged by adjacent chromosome.Topoisomerase IV Acts to disentangle the replicated intewined DNA strands by making double-strand breaks that allow one duplex to pass through the other.", 'role': 'assistant'}]