This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': "Which of the following is used for sequencing a long DNA fragment : \nSelect and explain. \nA. Sanger's technique, B. Chain termination method, C. Chromosome Walking, D. RFLP", 'role': 'user'}, {'content': "Answer: C \nExplanation: Chromosome walking Sequencing method of choice for sequencing long DNA fragments between 1.3 and 7 kb Applications of chromosome walking: Positional cloning Find, isolate, and clone a paicular allele Isolate a locus of interest for which no probe is available Analysis of genetically transmitted diseases Detect mutations Study SNPs (Single Nucleotide Polymorphisms) Disadvantage : Speed of this technique can't be enhanced", 'role': 'assistant'}]