This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': 'What does the term nick translation refer to? \nSelect and explain. \nA. Translation of RNA into polypeptide, B. Regulation of gene expression, C. Technique to label DNA, D. Technique to digest DNA', 'role': 'user'}, {'content': "Answer: C \nExplanation: A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5' to 3' direction. This propey is used to incorporate radio labeled nucleotides in DNA.", 'role': 'assistant'}]