This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': 'Real-time PCR is used for: \nSelect and explain. \nA. DNA detection only, B. RNA detection only, C. Protein detection only, D. For monitoring the amplification of target DNA', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Real-time PCR is used for monitoring the amplification of target DNA. In this PCR, we use SYBR-green dye, which has a propey of giving fluorescence when bound to dsDNA. When DNA synthesis is occurring then gradually ds DNA is increased in amount so fluorescence is also increased. This fluorescence can be measured and then amount of DNA can be calculated from that. Therefore it is known as Quantitative PCR (q PCR). Dyes used in qPCR: SYBR Based Detection: Uses SYBR Green dye (ds-DNA binding dye) - NOT sequence specific. TaqMan Based Detection: Uses a fluorogenic probe specific to target gene to detect target, as it accumulates during PCR.', 'role': 'assistant'}]