This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Molecular Genetics, Subject name: Biochemistry
[{'content': 'The enzyme used in polymerase chain reaction (PCR) is: \nSelect and explain. \nA. Taq polymerase, B. RNA polymerase, C. Ribonuclease, D. Endonuclease', 'role': 'user'}, {'content': "Answer: A \nExplanation: Ans. A. Taq polymerasePolymerase Chain Reaction is a technique employed for DNA amplification. It requires the following apparatus:a. A target DNA.b. Two primers (synthetic oligonucleotides of 17-30 nucleotides length) that are complementary to flanked regions of DNA.c. Four dNTP's.d. A DNA polymerase mainly Taq DNA polymerase (Thermo stable). It has been isolated form Thermus aquaticus. It is heat resistant, so no need to add fresh enzyme in every cycle.", 'role': 'assistant'}]