This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Glycolysis, Subject name: Biochemistry
[{'content': 'In Anaerobic glycolysis, there is gain of - \nSelect and explain. \nA. 2 ATP + 2 NAD, B. 2 ATP, C. 2 ATP + 2 NADH, D. 4 ATP + 2 FADH2', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Net gain in anaerobic glycolysis is only - 2 ATP. - 2 NADH are produced also (Glyceraldehyde-3-P dehydrogenase step) - But 2 NADH are used also (Lactate dehydrogenase step). Similarly - 2 NAD are produced also (Lactate dehydrogenase step) - But 2 NAD are used also (Glyceraldehyde-3-P dehydrogenase step). So there is no net gain of NADH or NAD.', 'role': 'assistant'}]