This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': "Which of the following method is used for the analysis of the C-terminal end of a polypeptide? \nSelect and explain. \nA. Sanger's method, B. Edman's degradation method, C. Akabori method, D. None of the above", 'role': 'user'}, {'content': "Answer: C \nExplanation: Akabori method is used for the analysis of C-terminal end of a polypeptide N-terminal analysis Carboxy-terminal analysis For post-translational modifications Sanger's reagent- fluoro-2,4 dinitrobenzene (FDNB) Carboxypeptidase assay Mass spectrometry Edman's reagent- Phenylisothiocyanate (PITC) Akabori reaction", 'role': 'assistant'}]