This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of nucleic acids, Subject name: Biochemistry
[{'content': 'In which of the following oligonucleotide primer is used? \nSelect and explain. \nA. RFLP, B. PCR, C. FISH, D. Chromosomal walking', 'role': 'user'}, {'content': 'Answer: B \nExplanation: DNA sequencing is used to determine the nucleotides in a DNA strand. The Sanger chain termination method of sequencing uses a primer to sta the chain reaction. In PCR, primers are used to determine the DNA fragment to be amplified by the PCR process. Ref-Sathyanarayana 4/e p386', 'role': 'assistant'}]