This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: All India exam, Subject name: Microbiology
[{'content': 'Viruses can be isolated from clinical samples by cultivation in the following, except \nSelect and explain. \nA. Animals, B. Embryonated eggs, C. Tissue culture, D. Chemically defined media', 'role': 'user'}, {'content': 'Answer: D \nExplanation: .', 'role': 'assistant'}]