This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Molecular Genetics, Subject name: Biochemistry
[{'content': 'Which of the following enzymes is used in Recombinant DNA research for Homopolymer tailing? \nSelect and explain. \nA. Reverse transcriptase, B. S1 Transferase, C. Polynucleotide kinase, D. Terminal transferase.', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Ans. D. Terminal transferaseSome of the Enzymes Used in Recombinant DNA ResearchEnzymeReactionPrimary UseAlkaline phosphataseDephosphorylates 5\' ends of RNA and DNARemoval of 5\'-PO4 groups prior to kinase labeling; also used to prevent self-ligationBAL 31 nucleaseDegrades both the 3\' and 5\' ends of DNAProgressive shortening of DNA moleculesDNA ligaseCatalyses bonds between DNA moleculesJoining of DNA moleculesDNA polymerase ISynthesizes double-stranded DNA from single- stranded DNASynthesis of double-stranded cDNA; nick translation; generation of blunt ends from sticky endsDNase IUnder appropriate conditions, produces single- stranded nicks in DNANick translation; mapping of hypersensitive sites; mapping protein-DNA interactionsExonuclease IIIRemoves nucleotides from 3\' ends of DNADNA sequencing; mapping of DNA-protein interactionsExonucleaseRemoves nucleotides from 5\' ends of DNADNA sequencingPolynucleotide kinaseTransfers terminal phosphate (position) from ATP to 5\'-OH groups of DNA or RNA32P end-labeling of DNA or RNAReverse transcriptaseSynthesizes DNA from RNA templateSynthesis of cDNA from mRNA; RNA (5\' end) mapping studiesS1 nucleaseDegrades single-stranded DNARemoval of "hairpin" in synthesis of cDNA; RNA mapping studies (both 5\' and 3\' ends)Terminal transferaseAdds nucleotides to the 3\' ends of DNAHomopolymer tailing', 'role': 'assistant'}]