This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Enzyme Kinetics, Subject name: Biochemistry
[{'content': 'Enzyme specificity is given by - \nSelect and explain. \nA. Km, B. Vmax, C. Both, D. None', 'role': 'user'}, {'content': 'Answer: A \nExplanation: Ans. is "a\' i.e., Km "The of an enzyme is the concentration of the substrate that enables the enzyme to function at half maximum activity and is therefore a measure of the specificity of a substrate for the enzyme"___________Clinical biochemistryo Actually enzyme specificity is not measured by Km alone.o It is measured by the ratio Kcat/Km which is a second order rate constant for the reaction between substrate and free enzyme.o This ratio is important, for it provides a direct measure of enzyme efficiency and specificity,o Note : Kcat is turnover number and measures the rate of the catalytic process.', 'role': 'assistant'}]