This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Glycogen, Subject name: Biochemistry
[{'content': 'Which of the following enzyme leads to release of free glucose from glycogen during glycogenolysis in muscle? \nSelect and explain. \nA. Glycogen phosphorylase, B. Glucose-1-phosphatase, C. Glucose-6-phosphatase, D. Debranching enzyme', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Debranching enzyme Has 2 catalytic sites in a single polypeptide chain. Glucan transferase and debranching enzyme are the two separate activities of the same enzyme. Glucan transferase 1,6-glycosidase transfers a trisaccharide unit from one branch to the other, exposing the 1 - 6 branch point. Breaks a (1- 6) bond, which is present at the staing of a branched-chain releasing glucose **Glucose-6-phosphatase also release free glucose but only in liver and not in muscle.', 'role': 'assistant'}]