This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'The following methods can be used to detect the point mutation in the beta (β)-globin gene that causes sickle cell anemia, except - \nSelect and explain. \nA. Polymerase chain reaction with allele-specific oligonucleotide hybridization, B. Southern blot analysis, C. DNA sequencing, D. Northern blot analysis', 'role': 'user'}, {'content': 'Answer: D \nExplanation: To detect point mutation, DNA analysis is required. While northern blot is a test for study of RNA.\nPolymerase chain reaction, DNA sequencing and southern blot testing are rapid methods for DNA analysis, and can all be used to detect the point mutation in the globin gene.', 'role': 'assistant'}]