This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
[{'content': 'Vaccines are sterilised by - \nSelect and explain. \nA. Seitz filtration, B. Hot air oven, C. Autoclaving, D. Heat inactivation', 'role': 'user'}, {'content': "Answer: D \nExplanation: Ans. is 'd' i.e., Heat inactivation . Vaccines of nonsporing bacteria are heat inactivated in special vaccine bath at 60degC for one hour.", 'role': 'assistant'}]