This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'All the following can be used to detect mutation, except: \nSelect and explain. \nA. Single strand conformational polymorphism, B. Ligase chain reaction, C. Polymerase chain reaction, D. DNA Sequencing', 'role': 'user'}, {'content': 'Answer: B \nExplanation: B i.e. Ligase chain reactionMutations can be detected by DNA sequencing PCR, PCR, single strand conformational polymorphism (SSCP), RFLP, and denaturing gradient gel electrophoresisQ.', 'role': 'assistant'}]