This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'All the following can be used to detect the mutation, except \nSelect and explain. \nA. Single strand conformational polymorphism, B. Ligase chain reaction, C. Polymerase chain reaction, D. DNA Sequencing', 'role': 'user'}, {'content': 'Answer: B \nExplanation: Methods used for detecting mutations\xa0\n\nCytogenetics analysis \xa0\nFISH \xa0\xa0\nPCR,\xa0\nRFLP\xa0\nDNA sequencing, Southern blotting\xa0\nMicroarrays\xa0\nSingle-stranded conformational polymorphism (SSCP)\nRNAase cleavage\nDenaturating gradient gel electrophoresis (DGGE)\nProtein truncation test', 'role': 'assistant'}]