This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of carbohydrate, Subject name: Biochemistry
[{'content': 'Rate limiting enzyme in glycolysis \nSelect and explain. \nA. Phosphofructokinase, B. Glucose 6 dehydrogenase, C. Pyruvate kinase, D. Pyruvate carboxylase', 'role': 'user'}, {'content': 'Answer: A \nExplanation: Fructose-6-phosphate is fuher phosphorylated to fructose1,6 bisphosphate. The enzyme is phosphofructokinase. is the rate-limiting enzyme of glycolysis. It is an allosterically regulated enzyme catalyzes the second phosphorylation step of glycolysis using a second molecule of ATP is an impoant key enzyme of this pathwayRef: DM Vasudevan, 7th edition, page no: 109', 'role': 'assistant'}]