This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
[{'content': 'Larger DNA segments can be cloned in \nSelect and explain. \nA. Plasmids, B. Bacteriophage, C. Cosmids, D. Bacterial Aificial Chromosomes', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Hybrid DNA molecules can be constructed in cloning vectors typically using bacterial plasmids, phages, or cosmids. A clone is a large population of identical molecules, bacteria, or cells that arise from a common ancestor. Molecular cloning allows for the production of a large number of identical DNA molecules, which can then be characterized or used for other purposes. Cloning capacities of vectors Plasmid 0.01-10 kb Lambda phage 10-20 kb Cosmids 35-50 kb Bacterial aificial chromosome 50-250 kb Yeast aificial chromosome 500-3000 kb', 'role': 'assistant'}]