This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Enzymes, Subject name: Biochemistry
[{'content': 'Carboxylases requires \nSelect and explain. \nA. Vitamin B12, B. Folic acid, C. Niacin, D. Biotin', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalyzed by a mitochondrial enzyme, pyruvate carboxylase. It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, page no: 103', 'role': 'assistant'}]