This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'Various biomedical tools are used for DNA technology. Which test uses oligomer with single base pair substitution? \nSelect and explain. \nA. PCR, B. RFLP, C. Error coded mutation analysis, D. FISH', 'role': 'user'}, {'content': "Answer: B \nExplanation: An inherited difference in the pattern of restriction enzyme digestion (eg, a DNA variation occurring in more than 1% of the general population) is known as a restriction fragment length polymorphism (RFLP). RFLPs result from single-base changes (eg, sickle-cell disease) or from deletions or inseions (CNVs) of DNA into a restriction fragment (eg, the thalassemias) and have proved to be useful diagnostic tools. Ref: Weil P. (2011). Chapter 39. Molecular Genetics, Recombinant DNA, & Genomic Technology. In D.A. Bender, K.M. Botham, P.A. Weil, P.J. Kennelly, R.K. Murray, V.W. Rodwell (Eds), Harper's Illustrated Biochemistry, 29e.", 'role': 'assistant'}]