This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Mycology, Subject name: Microbiology
[{'content': 'Which of the following fungi is difficult to isolate on REGULAR SDA media \nSelect and explain. \nA. Candida, B. Dermatophytes, C. Cryptococcus, D. Malassezia furfur', 'role': 'user'}, {'content': "Answer: D \nExplanation: M. furfur requires fatty acids for growth and does not grow on regular Sabouraud's medium Hence is cultured on the Sabouraud's dextrose agar (SDA) overlayed with a layer of olive oil. On incubation at 37degC, the fungus produces creamy colonies or Fried egg colonies within 5-7 days. Candida, Dermatophytes, Cryptococcus can be isolated easily on SDA.", 'role': 'assistant'}]