This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'True regarding electrophoresis are all except \nSelect and explain. \nA. Isoelectric focusing use ampholytes & pI, B. Adversely affect structure & function, C. Depends on size not on shape, D. Commonest method of purifying protein', 'role': 'user'}, {'content': 'Answer: C \nExplanation: Electrophoresis depends on size as well as on shape\nElectrophoresis is the process that uses an electric field to drive the movement of charged particles. Electrophoresis depends on the net charge of the particle, molecular weight, pH of the medium, the strength of the electric field and temperature.\n\nAbout other options\n\nAmpholyte is used in isoelectric focusing to create a pH gradient across the supporting media. Molecules stop migrating as soon as they reach their respective isoelectric point (pI).\n\n\n\nElectrophoresis can adversely affect the structure and function of proteins.\nElectrophoresis is the most common method for protein separation.\n"Electrophoresis is the most common method of protein separation in the clinical laboratory".\xa0\n"PAGE and SDS-PAGE are extensively used for characterization and purification of tissue proteins, enzyme, receptors, and surface antigens".\n\nElectrophoresis uses an electric field', 'role': 'assistant'}]