This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Structure and function of protein, Subject name: Biochemistry
[{'content': 'Protein purification and separation can be done by all except \nSelect and explain. \nA. Chromatography, B. Centrifugation, C. Electrophoresis, D. None', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Techniques for protein separation:ChromatographyColumn ChromatographyPaition ChromatographySize Exclusion ChromatographyAdsorption ChromatographyIon Exchange ChromatographyAffinity ChromatographyHigh-Pressure Liquid ChromatographyGas ChromatographyPAGEIEFSalting out with ammonia sulfateRef: Harper 28th Ed. Page-21, Harper 27th Ed. Page: 21', 'role': 'assistant'}]