This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
[{'content': 'The following methods can be used to detect the point mutation in the beta globin gene that causes sickle cell anemia, EXCEPT: \nSelect and explain. \nA. Polymerase chain reaction with allele-specific oligonucleotide hybridization, B. Southern blot analysis, C. DNA sequencing, D. Nohern blot analysis', 'role': 'user'}, {'content': 'Answer: D \nExplanation: Point mutation involves the replacement of a single base nucleotide with another nucleotide of the gene. Point mutations in the beta globin gene can be identified by analysing the DNA. Nohern blot is a technique used in studying the RNA and hence the answer of exclusion. Ref: Introduction to Biotechnology By Thieman William, 2nd Edition, Pages 71, 78, 81, 82', 'role': 'assistant'}]