<jupyter_start><jupyter_text>pYPKp4The pYPKp4 is a version of the pYPK4 with a deletion in the crp gene that originates from the pCAPs vector.<jupyter_code>from pydna.all import *

pYPK4 =read("pYPK4.gb")
f,r =parse(''' >866_pYPKpwF (49-mer)
                      GCATGATATCttcacaggcggttttcgcacgtacccatg

                      >865_pYPKpwR (49-mer)
                      GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)

seq =pcr(f, r, pYPK4)

seq = seq.looped()

pYPKp4 = seq.synced(pYPK4)

pYPKp4 = seq.synced(pYPK4)

pYPKp4.stamp()
pYPKp4.locus = "pYPKp4"
pYPKp4.write("pYPKp4.gb")<jupyter_output><empty_output><jupyter_text>[DOWNLOAD](pYPKp4.gb)<jupyter_code>from pydna.all import *
reloaded=read("pYPKp4.gb")
assert reloaded.cseguid() in reloaded.definition<jupyter_output><empty_output>
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