<commit_before>Picogreen Protocol 
==================

## Authorship

<NAME> and <NAME> (2011-2014)


## Printing this protocol

* View this Markdown file on GitHub, then in your URL bar of your browser 
  replace the git**hub**.com part of the URL with git**print**.com
* The Markdown file will be rendered as a PDF for easy printing or downloading.


## Notes

* The protocol assumes one 96-well plate.

## Materials

* \(1\) Costar black 96-well plate
* \(8\) 1.5ml micro-cfg tubes
* \(2\) 15 or 50 ml falcon tubes (depends on volume 1x TE buffer neded)
* \(1\) Picogreen kit
	* Warm up the picogreen reagent before use<commit_msg>updated picogreen for robot method<commit_after>Picogreen Protocol 
==================

## Authorship

<NAME> and <NAME> (2011-2014)


## Printing this protocol

See **Printing protocols** in the [README](../README.md)

* View this Markdown file on GitHub, then in your URL bar of your browser 
  replace the git**hub**.com part of the URL with git**print**.com
* The Markdown file will be rendered as a PDF for easy printing or downloading.


# Automated Picogreen protocol (using the Hamilton robot)

## Basic method

* Select the correct robot method
* The method will prompt you for all of the materials needed
  (e.g., picogreen & DNA standard)
* Run the method in simulation mode to get an idea of what to expect.

## Notes on CsCl gradient fractionation quantification

* Using 2 ul of each fraction per Picogreen rxn should be adequate, 
even at the ends of the gradient.
* Use 5 ul of each fraction if you want higher precision. 


# Manual Picogreen protocol

## Notes

* The protocol assumes one 96-well plate.

## Materials

* \(1\) Costar black 96-well plate
* \(8\) 1.5ml micro-cfg tubes
* \(2\) 15 or 50 ml falcon tubes (depends on volume 1x TE buffer neded)
* \(1\) Picogreen kit
	* Warm up the picogreen reagent before use
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