<commit_before>---
layout: page_single
title: Thanks for checking out my poster!
permalink: /anaerobe/
comments: false
image:
  feature: anaerobe2016.jpg
---

My work in the [Schloss lab](http://www.schlosslab.org/) leverages metagenomics, metatranscriptomics, and metabolic modeling to identify the nutrient niche of *Clostridium difficile* in the gut during infection.  With this information we can potentially begin to design targeted probiotics to eliminate/prevent colonization of *C. difficile*.  Below is some supplementary info for my poster, it will give you some background on how I generated the data and where I got some of my ideas.  For more about me or my science, take a look at my [website](http://mjenior.github.io)!

---

Methods (Abbreviated)
---------------------

**Differential plating:**

All done anaerobically*
Vegetative cells = plated on CCFA (no taurocholate)
Spores = 60 degrees Celsius for 30 minutes, then plated on TCCFA


**Toxin titer:**

Performed using the standard cell rounding assay by [<NAME>](https://www.researchgate.net/profile/Jhansi_Leslie) in [Vince Young's lab](https://sites.google.com/a/umich.edu/younglab/).


**High-throughpt sequencing:**

Illumina MiSeq - 16S rRNA gene (2 x 250 bp)<commit_msg>formatting anaerobe page<commit_after>---
layout: page_single
title: Thanks for checking out my poster!
permalink: /anaerobe/
comments: false
image:
  feature: anaerobe2016.jpg
---

My work in the [Schloss lab](http://www.schlosslab.org/) leverages metagenomics, metatranscriptomics, and metabolic modeling to identify the nutrient niche of *Clostridium difficile* in the gut during infection.  With this information we can potentially begin to design targeted probiotics to eliminate/prevent colonization of *C. difficile*.  Below is some supplementary info for my poster, it will give you some background on how I generated the data and where I got some of my ideas.  For more about me or my science, take a look at my [website](http://mjenior.github.io)!

---

Methods (Abbreviated)
---------------------

**Differential plating:**

All done anaerobically*

Vegetative cells = plated on CCFA (no taurocholate)

Spores = 60 degrees Celsius for 30 minutes, then plated on TCCFA


**Toxin titer:**

Performed using the standard cell rounding assay by [<NAME>](https://www.researchgate.net/profile/Jhansi_Leslie) in [Vince Young's lab](https://sites.google.com/a/umich.edu/younglab/).


**High-throughpt sequencing:**

Illumina MiSeq - 16S rRNA gene (2 x 250 bp)

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