<commit_before>#SampleID	BarcodeSequence	LinkerPrimerSequence	center_name	center_project_name	emp_status	experiment_center	experiment_design_description	experiment_title	illumina_technology	instrument_model	library_construction_protocol	pcr_primers	platform	run_center	run_date	run_prefix	samp_size	sample_center	sequencing_meth	study_center	target_gene	target_subfragment	altitude	anonymized_name	assigned_from_geo	collection_timestamp	common_name	country	depth	description_duplicate	elevation	env_biome	env_feature	has_extracted_data	has_physical_specimen	host_subject_id	host_taxid	latitude	longitude	ph	physical_location	samp_salinity	sample_type	season_environment	taxon_id	temp	texture	tot_nitro	tot_org_carb	water_content_soil	Description
1.SKB1.640202	GTCCGCAAGTTA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB1	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Burmese bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M2	3483	4.59216095574	63.5115213108	6.94	ANL	7.15	ENVO:soil	winter	410658	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB2.640194	CGTAGAGCTCTC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB2	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Burmese bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B4	3483	35.2374368957	68.5041623253	6.94	ANL	7.15	ENVO:soil	winter	410658	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB3.640195	CCTCTGAGAGCT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB3	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Burmese bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M6	3483	95.20607497479999	27.3592668624	6.94	ANL	7.15	ENVO:soil	winter	410658	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB4.640189	CCTCGATGCAGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB4	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Burmese Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D7	3483	43.961471519700005	82.8516734159	6.94	ANL	7.15	ENVO:soil	winter	939928	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB5.640181	GCGGACTATTCA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB5	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Burmese Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M4	3483	10.665559909299999	70.784770579	6.94	ANL	7.15	ENVO:soil	winter	939928	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB6.640176	CGTGCACAATTG	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB6	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Burmese Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D5	3483	78.3634273709	74.423907894	6.94	ANL	7.15	ENVO:soil	winter	939928	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB7.640196	CGGCCTAAGTTC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB7	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Burmese root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M8	3483	13.089194595	92.5274472082	6.94	ANL	7.15	ENVO:soil	winter	1118232	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB8.640193	AGCGCTCACATC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB8	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Burmese root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M7	3483	74.0894932572	65.3283470202	6.94	ANL	7.15	ENVO:soil	winter	1118232	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB9.640200	TGGTTATGGCAC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB9	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Burmese root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B3	3483	12.6245524972	96.0693176066	6.8	ANL	7.15	ENVO:soil	winter	1118232	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKD1.640179	CGAGGTTCTGAT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD1	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Diesel bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M5	3483	68.0991287718	34.8360987059	6.8	ANL	7.1	ENVO:soil	winter	410658	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD2.640178	AACTCCTGTGGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD2	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Diesel bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B5	3483	53.505069239499996	31.6056761814	6.8	ANL	7.1	ENVO:soil	winter	410658	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD3.640198	TAATGGTCGTAG	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD3	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Diesel bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B1	3483	84.00302275850001	66.8954849864	6.8	ANL	7.1	ENVO:soil	winter	410658	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD4.640185	TTGCACCGTCGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD4	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Diesel Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M9	3483	40.8623799474	6.664442201869999	6.8	ANL	7.1	ENVO:soil	winter	939928	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD5.640186	TGCTACAGACGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD5	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Diesel Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M1	3483	85.4121476399	15.6526750776	6.8	ANL	7.1	ENVO:soil	winter	939928	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD6.640190	ATGGCCTGACTA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD6	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Diesel Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B9	3483	29.149946069200002	82.12704182270001	6.8	ANL	7.1	ENVO:soil	winter	939928	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD7.640191	ACGCACATACAA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD7	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Diesel Root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D6	3483	68.51099627	2.35063674718	6.8	ANL	7.1	ENVO:soil	winter	1118232	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD8.640184	TGAGTGGTCTGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD8	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Diesel Root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D9	3483	57.571893782	32.5563076447	6.8	ANL	7.1	ENVO:soil	winter	1118232	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD9.640182	GATAGCACTCGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD9	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Diesel Root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D3	3483	23.121803279899996	42.838497795	6.82	ANL	7.1	ENVO:soil	winter	1118232	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKM1.640183	TAGCGCGAACTT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM1	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Bucu bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D1	3483	38.2627021402	3.4827426421900003	6.82	ANL	7.44	ENVO:soil	winter	410658	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM2.640199	CATACACGCACC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM2	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Bucu bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D4	3483	82.8302905615	86.3615778099	6.82	ANL	7.44	ENVO:soil	winter	410658	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM3.640197	ACCTCAGTCAAG	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM3	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Bucu bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B7	3483	63.6505562766	31.200347458499998	6.82	ANL	7.44	ENVO:soil	winter	410658	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM4.640180	TCGACCAAACAC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM4	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Bucu Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D2	3483	31.7167821863	95.50885660870001	6.82	ANL	7.44	ENVO:soil	winter	939928	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM5.640177	CCACCCAGTAAC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM5	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Bucu Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M3	3483	44.9725384282	66.19200146989999	6.82	ANL	7.44	ENVO:soil	winter	939928	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM6.640187	ATATCGCGATGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM6	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Bucu Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B2	3483	0.29186763591299997	68.5945325743	6.82	ANL	7.44	ENVO:soil	winter	939928	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM7.640188	CGCCGGTAATCT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM7	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Bucu Roots	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B6	3483	60.1102854322	74.71232483819999	6.82	ANL	7.44	ENVO:soil	winter	1118232	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM8.640201	CCGATGCCTTGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM8	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Bucu Roots	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D8	3483	3.21190859967	26.8138925876	6.82	ANL	7.44	ENVO:soil	winter	1118232	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
1.SKM9.640192	AGCAGGCACGAA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM9	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Bucu Roots	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B8	3483	12.706595771400002	84.9722975792	6.82	ANL	7.44	ENVO:soil	winter	1118232	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.1001	Cannabis Soil Microbiome
<commit_msg>fixing error<commit_after>#SampleID	BarcodeSequence	LinkerPrimerSequence	center_name	center_project_name	emp_status	experiment_center	experiment_design_description	experiment_title	illumina_technology	instrument_model	library_construction_protocol	pcr_primers	platform	run_center	run_date	run_prefix	samp_size	sample_center	sequencing_meth	study_center	target_gene	target_subfragment	altitude	anonymized_name	assigned_from_geo	collection_timestamp	common_name	country	depth	description_duplicate	elevation	env_biome	env_feature	has_extracted_data	has_physical_specimen	host_subject_id	host_taxid	latitude	longitude	ph	physical_location	samp_salinity	sample_type	season_environment	taxon_id	temp	texture	tot_nitro	tot_org_carb	water_content_soil	Description
1.SKB1.640202	GTCCGCAAGTTA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB1	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Burmese bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M2	3483	4.59216095574	63.5115213108	6.94	ANL	7.15	ENVO:soil	winter	410658	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB2.640194	CGTAGAGCTCTC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB2	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Burmese bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B4	3483	35.2374368957	68.5041623253	6.94	ANL	7.15	ENVO:soil	winter	410658	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB3.640195	CCTCTGAGAGCT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB3	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Burmese bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M6	3483	95.20607497479999	27.3592668624	6.94	ANL	7.15	ENVO:soil	winter	410658	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB4.640189	CCTCGATGCAGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB4	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Burmese Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D7	3483	43.961471519700005	82.8516734159	6.94	ANL	7.15	ENVO:soil	winter	939928	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB5.640181	GCGGACTATTCA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB5	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Burmese Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M4	3483	10.665559909299999	70.784770579	6.94	ANL	7.15	ENVO:soil	winter	939928	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB6.640176	CGTGCACAATTG	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB6	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Burmese Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D5	3483	78.3634273709	74.423907894	6.94	ANL	7.15	ENVO:soil	winter	939928	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB7.640196	CGGCCTAAGTTC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB7	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Burmese root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M8	3483	13.089194595	92.5274472082	6.94	ANL	7.15	ENVO:soil	winter	1118232	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB8.640193	AGCGCTCACATC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB8	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Burmese root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M7	3483	74.0894932572	65.3283470202	6.94	ANL	7.15	ENVO:soil	winter	1118232	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKB9.640200	TGGTTATGGCAC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKB9	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Burmese root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B3	3483	12.6245524972	96.0693176066	6.8	ANL	7.15	ENVO:soil	winter	1118232	15.0	64.6 sand, 17.6 silt, 17.8 clay	1.41	5.0	0.164	Cannabis Soil Microbiome
1.SKD1.640179	CGAGGTTCTGAT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD1	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Diesel bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M5	3483	68.0991287718	34.8360987059	6.8	ANL	7.1	ENVO:soil	winter	410658	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD2.640178	AACTCCTGTGGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD2	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Diesel bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B5	3483	53.505069239499996	31.6056761814	6.8	ANL	7.1	ENVO:soil	winter	410658	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD3.640198	TAATGGTCGTAG	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD3	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Diesel bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B1	3483	84.00302275850001	66.8954849864	6.8	ANL	7.1	ENVO:soil	winter	410658	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD4.640185	TTGCACCGTCGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD4	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Diesel Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M9	3483	40.8623799474	6.664442201869999	6.8	ANL	7.1	ENVO:soil	winter	939928	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD5.640186	TGCTACAGACGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD5	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Diesel Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M1	3483	85.4121476399	15.6526750776	6.8	ANL	7.1	ENVO:soil	winter	939928	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD6.640190	ATGGCCTGACTA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD6	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Diesel Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B9	3483	29.149946069200002	82.12704182270001	6.8	ANL	7.1	ENVO:soil	winter	939928	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD7.640191	ACGCACATACAA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD7	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Diesel Root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D6	3483	68.51099627	2.35063674718	6.8	ANL	7.1	ENVO:soil	winter	1118232	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD8.640184	TGAGTGGTCTGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD8	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Diesel Root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D9	3483	57.571893782	32.5563076447	6.8	ANL	7.1	ENVO:soil	winter	1118232	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKD9.640182	GATAGCACTCGT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKD9	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Diesel Root	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D3	3483	23.121803279899996	42.838497795	6.82	ANL	7.1	ENVO:soil	winter	1118232	15.0	66 sand, 16.3 silt, 17.7 clay	1.51	4.32	0.178	Cannabis Soil Microbiome
1.SKM1.640183	TAGCGCGAACTT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM1	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Bucu bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D1	3483	38.2627021402	3.4827426421900003	6.82	ANL	7.44	ENVO:soil	winter	410658	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM2.640199	CATACACGCACC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM2	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Bucu bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D4	3483	82.8302905615	86.3615778099	6.82	ANL	7.44	ENVO:soil	winter	410658	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM3.640197	ACCTCAGTCAAG	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM3	n	2011-11-11 13:00:00	soil metagenome	GAZ:United States of America	0.15	Bucu bulk	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B7	3483	63.6505562766	31.200347458499998	6.82	ANL	7.44	ENVO:soil	winter	410658	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM4.640180	TCGACCAAACAC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM4	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Bucu Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D2	3483	31.7167821863	95.50885660870001	6.82	ANL	7.44	ENVO:soil	winter	939928	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM5.640177	CCACCCAGTAAC	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM5	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Bucu Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:M3	3483	44.9725384282	66.19200146989999	6.82	ANL	7.44	ENVO:soil	winter	939928	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM6.640187	ATATCGCGATGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM6	n	2011-11-11 13:00:00	rhizosphere metagenome	GAZ:United States of America	0.15	Bucu Rhizo	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B2	3483	0.29186763591299997	68.5945325743	6.82	ANL	7.44	ENVO:soil	winter	939928	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM7.640188	CGCCGGTAATCT	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM7	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Bucu Roots	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B6	3483	60.1102854322	74.71232483819999	6.82	ANL	7.44	ENVO:soil	winter	1118232	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM8.640201	CCGATGCCTTGA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM8	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Bucu Roots	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:D8	3483	3.21190859967	26.8138925876	6.82	ANL	7.44	ENVO:soil	winter	1118232	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome
1.SKM9.640192	AGCAGGCACGAA	GTGCCAGCMGCCGCGGTAA	ANL		EMP	ANL	micro biome of soil and rhizosphere of cannabis plants from CA	Cannabis Soil Microbiome	MiSeq	Illumina MiSeq	This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.	FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT	Illumina	ANL	8/1/12	s_G1_L001_sequences	.25,g	ANL	Sequencing by synthesis	CCME	16S rRNA	V4	0.0	SKM9	n	2011-11-11 13:00:00	root metagenome	GAZ:United States of America	0.15	Bucu Roots	114.0	ENVO:Temperate grasslands, savannas, and shrubland biome	ENVO:plant-associated habitat	True	True	1001:B8	3483	12.706595771400002	84.9722975792	6.82	ANL	7.44	ENVO:soil	winter	1118232	15.0	63.1 sand, 17.7 silt, 19.2 clay	1.3	3.31	0.101	Cannabis Soil Microbiome

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