<commit_before>- name: Semi-Quantitative Brood Assay
  file: SemiQuantitativeBroodAssay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: <em>Pseudomonas aeruginosa</em> Fast-killing assay</a>
  file: FKAprotocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: <em>Staphylococcus aureus</em> killing assay</a>
  file: Staphaureus_Protocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: <em>Bacillus thuringiensis</em> toxin assay on plates</a>
  file: Bacillus-thuringiensis-toxin-plate-assay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: L1 arrest survival
  file: Quantitative-L1-arrest-protocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: COPAS Conversion of Bubble Trap
  file: ConvertBubbletoNonBubble.pdf
  group: COPAS BIOSORT
- name: Yeast-mediated ligation plasmid construction
  file: yeast-mediated-ligation-protocol.pdf
  group: DNA Methods
- name: 96-Well DNAPrep - Omega Bio-tek
  file: 96wellDNAPrepOmega.pdf
  group: DNA Methods
- name: 96-Well High-multiplexing Tagmentation and Amplification
  file: 96-well High-multiplexing Tagmentation and Amplification.pdf
  group: DNA Methods
- name: Generating Libraries for Whole-Genome Sequencing
  file: NexteraLibraryGenerationLowMultiplexingTagmentation.pdf
  group: DNA Methods
- name: Genome Editing Cas9 Protein Microinjection
  file: GenomeEditing_Cas9ProteinMicroinjection.pdf
  group: DNA Methods
- name: Qiagen Gentra DNA preparation for C. elegans
  file: PurificationDNAfromnematodes.pdf
  group: DNA Methods
- name: DNAEasy Genomic Prep
  file: DNAEasyGenomicDNAPrep.pdf
  group: DNA Methods
- name: 96-Well Quantification Of DNA
  file: 96-wellDNAQuantification_Synergy4_Qubit_v2.pdf
  group: DNA Methods
- name: 96-Well DNAPrep - Gentra Purgene
  file: 96-wellDNAPrepGentra.pdf
  group: DNA Methods
- name: EMS mutagenesis
  file: EMS-mutagenesis.pdf
  group: General <em>C. elegans</em>
- name: Superbroth and HB101 growth
  file: Superbroth-and-HB101-growth.pdf
  group: General <em>C. elegans</em>
- name: Methanol/Acetone fixation for Whole-mount staining
  file: MethanolAcetone-Fixation.pdf
  group: General <em>C. elegans</em>
- name: Chunking Worms
  file: ChunkingWorms.pdf
  group: General <em>C. elegans</em>
- name: Flow Chart For A Cross
  file: IntrogressionCrossFlowChart.pdf
  group: General <em>C. elegans</em>
- name: Isolating Wild Isolates
  file: IsolatingWildIsolates.pdf
  group: General <em>C. elegans</em>
- name: Single Worm Genotyping
  file: SingleWormGenotyping.pdf
  group: General <em>C. elegans</em>
- name: Defecation Assay
  file: 20080221_DefecationAssay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: Microinjection
  file: Microinjection.pdf
  group: General <em>C. elegans</em>
- name: Cleaning Worms
  file: CleaningWorms.pdf
  group: General <em>C. elegans</em>
- name: Soft Agar Freezing and Thawing
  file: SoftAgarFreezing&Thawing.pdf
  group: General <em>C. elegans</em>
- name: How to Build a NIL
  file: HowToBuildaNIL.pdf
  group: General <em>C. elegans</em>
- name: Synchronizing Worms by Bleaching
  file: SynchronizingWormsbyBleaching.pdf
  group: General <em>C. elegans</em>
- name: Crude Dauer Pheromone Prep
  file: CrudeDauerPheromonePrep.pdf
  group: General <em>C. elegans</em>
- name: Growing OP50 Cultures for Seeding Plates
  file: GrowingOP50CulturesforSeedingPlates.pdf
  group: General <em>C. elegans</em>
- name: Seeding NGMA Plates With Bacteria
  file: SeedingNGMAPlatesWithBacteria.pdf
  group: General <em>C. elegans</em>
- name: Bleaching and Dispensing of C. elegans using the BioTek MicroFlo Select
  file: 96-well bleaching and automated dispensing.pdf
  group: General <em>C. elegans</em>
- name: How To Make Cholesterol
  file: HowToMakeCholesterol.pdf
  group: General Laboratory Methods
- name: Media and Solutions Recipes
  file: MediaAndSolutionsRecipes.pdf
  group: General Laboratory Methods
- name: How to Dilute DNA Ladder
  file: HowtodiluteDNAladder.pdf
  group: General Laboratory Methods
- name: Autoclave Sterilizing Liquids & Labware
  file: AutoclaveSterilizingLiquids&Labware.pdf
  group: General Laboratory Methods
- name: Washing Labware
  file: WashingProtocols.pdf
  group: General Laboratory Methods
- name: Pouring NGMA Plates Using the Automatic Plate Pourer
  file: PlatePouring.pdf
  group: General Laboratory Methods
- name: Concentrated M9 Recipe
  file: ConcentratedM9Recipe.pdf
  group: General Laboratory Methods
- name: Using BioTek Microplate Dispensor
  file: UsingBioTekMicroplateDispensor.pdf
  group: General Laboratory Methods
- name: Set Up 96-Well Aspirator
  file: SetUp96WellAspirator.pdf
  group: General Laboratory Methods
- name: RNA synthesis and annealing for RNAi injection
  file: RNA-synthesis-and-annealing-for-RNAi-injection.pdf
  group: RNA Methods
- name: Quantitative Western Blotting
  file: Quantitative-Western-Blot-Protocol.pdf
  group: Protein Methods
- name: Synthetic sgRNA
  file: 20190524_synthego-quickstart-sgrna.pdf
  group: DNA Methods
- name: Andersen Lab Strain Intake
  file: 20190624_WildIsolateStrainIntake.pdf
  group: General <em>C. elegans</em>
- name: Worm Maintenance
  file: 20191005_WormMaintenance.pdf
  group: General <em>C. elegans</em>
- name: High Throughput Genotyping for Wild Isolate Identification
  file: 20200429_HighthroughputGenotyping.pdf
  group: DNA Methods
- name: CTAB DNA Prep of A. dissimilis
  file: CTABDNAPrep.pdf
  group: DNA Methods
- name: Identifying New Wild Isolates
  file: 20210426_IdentifyingWildIsolates.pdf
  group: General <em>C. elegans</em>
- name: Andersen Lab COPAS Sorter Best Practices
  file: AndersenLabCOPASSorterBestPractices.pdf
  group: COPAS BIOSORT
- name: Andersen Lab Nematode Field Sampling Protocol
  file: AndersenLabNematodeFieldSamplingProtocol.pdf
  group: General <em>C. elegans</em>
- name: Nematode Collection Protocol
  file: NematodeCollectionProtocol.pdf
  group: General <em>C. elegans</em>
- name: Sorter Setup, Shutdown and Sterilization
  file: SorterSetupandShutdown.pdf
  group: COPAS BIOSORT
- name: Tips for Using the COPAS BIOSORT
  file: 20160611_TipsforUsingCOPASBiosort.pdf
  group: COPAS BIOSORT
- name: Worm Collecting Protocol - Citizen Scientist
  file: WormCollectionProtocol.pdf
  group: General <em>C. elegans</em>
- name: 15-hour Bacterial Food Growth
  file: 20221107_BacterialFoodGrowthProtocol.pdf
  group: General <em>C. elegans</em>
- name: CRISPR-Cas9 Genome Editing by RNP Injection
  file: 20221107_CRISPR-Cas9Genome-EditingProtocol.pdf
  group: DNA Methods
- name: Cryopreservation and DNA Preps of Caenorhabditis Nematodes
  file: 20221107_CryopreservationandDNAPrepsofCaenorhabditisNematodes.pdf
  group: General <em>C. elegans</em>
- name: High-throughput Phenotyping Assay - SORTER
  file: 20221107_High-throughputSorterAssayProtocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods<commit_msg>Update protocols.yaml<commit_after>- name: Semi-Quantitative Brood Assay
  file: SemiQuantitativeBroodAssay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: <em>Pseudomonas aeruginosa</em> Fast-killing assay</a>
  file: FKAprotocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: <em>Staphylococcus aureus</em> killing assay</a>
  file: Staphaureus_Protocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: <em>Bacillus thuringiensis</em> toxin assay on plates</a>
  file: Bacillus-thuringiensis-toxin-plate-assay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: L1 arrest survival
  file: Quantitative-L1-arrest-protocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: COPAS Conversion of Bubble Trap
  file: ConvertBubbletoNonBubble.pdf
  group: COPAS BIOSORT
- name: Yeast-mediated ligation plasmid construction
  file: yeast-mediated-ligation-protocol.pdf
  group: DNA Methods
- name: 96-Well DNAPrep - Omega Bio-tek
  file: 96wellDNAPrepOmega.pdf
  group: DNA Methods
- name: 96-Well High-multiplexing Tagmentation and Amplification
  file: 96-well High-multiplexing Tagmentation and Amplification.pdf
  group: DNA Methods
- name: Generating Libraries for Whole-Genome Sequencing
  file: NexteraLibraryGenerationLowMultiplexingTagmentation.pdf
  group: DNA Methods
- name: Genome Editing Cas9 Protein Microinjection
  file: GenomeEditing_Cas9ProteinMicroinjection.pdf
  group: DNA Methods
- name: Qiagen Gentra DNA preparation for C. elegans
  file: PurificationDNAfromnematodes.pdf
  group: DNA Methods
- name: DNAEasy Genomic Prep
  file: DNAEasyGenomicDNAPrep.pdf
  group: DNA Methods
- name: 96-Well Quantification Of DNA
  file: 96-wellDNAQuantification_Synergy4_Qubit_v2.pdf
  group: DNA Methods
- name: 96-Well DNAPrep - Gentra Purgene
  file: 96-wellDNAPrepGentra.pdf
  group: DNA Methods
- name: EMS mutagenesis
  file: EMS-mutagenesis.pdf
  group: General <em>C. elegans</em>
- name: Superbroth and HB101 growth
  file: Superbroth-and-HB101-growth.pdf
  group: General <em>C. elegans</em>
- name: Methanol/Acetone fixation for Whole-mount staining
  file: MethanolAcetone-Fixation.pdf
  group: General <em>C. elegans</em>
- name: Chunking Worms
  file: ChunkingWorms.pdf
  group: General <em>C. elegans</em>
- name: Flow Chart For A Cross
  file: IntrogressionCrossFlowChart.pdf
  group: General <em>C. elegans</em>
- name: Single Worm Genotyping
  file: SingleWormGenotyping.pdf
  group: General <em>C. elegans</em>
- name: Defecation Assay
  file: 20080221_DefecationAssay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: Microinjection
  file: Microinjection.pdf
  group: General <em>C. elegans</em>
- name: Cleaning Worms
  file: CleaningWorms.pdf
  group: General <em>C. elegans</em>
- name: Soft Agar Freezing and Thawing
  file: SoftAgarFreezing&Thawing.pdf
  group: General <em>C. elegans</em>
- name: How to Build a NIL
  file: HowToBuildaNIL.pdf
  group: General <em>C. elegans</em>
- name: Synchronizing Worms by Bleaching
  file: SynchronizingWormsbyBleaching.pdf
  group: General <em>C. elegans</em>
- name: Crude Dauer Pheromone Prep
  file: CrudeDauerPheromonePrep.pdf
  group: General <em>C. elegans</em>
- name: Growing OP50 Cultures for Seeding Plates
  file: GrowingOP50CulturesforSeedingPlates.pdf
  group: General <em>C. elegans</em>
- name: Seeding NGMA Plates With Bacteria
  file: SeedingNGMAPlatesWithBacteria.pdf
  group: General <em>C. elegans</em>
- name: Bleaching and Dispensing of C. elegans using the BioTek MicroFlo Select
  file: 96-well bleaching and automated dispensing.pdf
  group: General <em>C. elegans</em>
- name: How To Make Cholesterol
  file: HowToMakeCholesterol.pdf
  group: General Laboratory Methods
- name: Media and Solutions Recipes
  file: MediaAndSolutionsRecipes.pdf
  group: General Laboratory Methods
- name: How to Dilute DNA Ladder
  file: HowtodiluteDNAladder.pdf
  group: General Laboratory Methods
- name: Autoclave Sterilizing Liquids & Labware
  file: AutoclaveSterilizingLiquids&Labware.pdf
  group: General Laboratory Methods
- name: Washing Labware
  file: WashingProtocols.pdf
  group: General Laboratory Methods
- name: Pouring NGMA Plates Using the Automatic Plate Pourer
  file: PlatePouring.pdf
  group: General Laboratory Methods
- name: Concentrated M9 Recipe
  file: ConcentratedM9Recipe.pdf
  group: General Laboratory Methods
- name: Using BioTek Microplate Dispensor
  file: UsingBioTekMicroplateDispensor.pdf
  group: General Laboratory Methods
- name: Set Up 96-Well Aspirator
  file: SetUp96WellAspirator.pdf
  group: General Laboratory Methods
- name: RNA synthesis and annealing for RNAi injection
  file: RNA-synthesis-and-annealing-for-RNAi-injection.pdf
  group: RNA Methods
- name: Quantitative Western Blotting
  file: Quantitative-Western-Blot-Protocol.pdf
  group: Protein Methods
- name: Synthetic sgRNA
  file: 20190524_synthego-quickstart-sgrna.pdf
  group: DNA Methods
- name: Andersen Lab Strain Intake
  file: 20190624_WildIsolateStrainIntake.pdf
  group: General <em>C. elegans</em>
- name: Worm Maintenance
  file: 20191005_WormMaintenance.pdf
  group: General <em>C. elegans</em>
- name: High Throughput Genotyping for Wild Isolate Identification
  file: 20200429_HighthroughputGenotyping.pdf
  group: DNA Methods
- name: CTAB DNA Prep of A. dissimilis
  file: CTABDNAPrep.pdf
  group: DNA Methods
- name: Identifying New Wild Isolates
  file: 20210426_IdentifyingWildIsolates.pdf
  group: General <em>C. elegans</em>
- name: Andersen Lab COPAS Sorter Best Practices
  file: AndersenLabCOPASSorterBestPractices.pdf
  group: COPAS BIOSORT
- name: Andersen Lab Nematode Field Sampling Protocol
  file: AndersenLabNematodeFieldSamplingProtocol.pdf
  group: General <em>C. elegans</em>
- name: Nematode Collection Protocol
  file: NematodeCollectionProtocol.pdf
  group: General <em>C. elegans</em>
- name: Sorter Setup, Shutdown and Sterilization
  file: SorterSetupandShutdown.pdf
  group: COPAS BIOSORT
- name: Tips for Using the COPAS BIOSORT
  file: 20160611_TipsforUsingCOPASBiosort.pdf
  group: COPAS BIOSORT
- name: Worm Collecting Protocol - Citizen Scientist
  file: WormCollectionProtocol.pdf
  group: General <em>C. elegans</em>
- name: 15-hour Bacterial Food Growth
  file: 20221107_BacterialFoodGrowthProtocol.pdf
  group: General <em>C. elegans</em>
- name: CRISPR-Cas9 Genome Editing by RNP Injection
  file: 20221107_CRISPR-Cas9Genome-EditingProtocol.pdf
  group: DNA Methods
- name: Cryopreservation and DNA Preps of Caenorhabditis Nematodes
  file: 20221107_CryopreservationandDNAPrepsofCaenorhabditisNematodes.pdf
  group: General <em>C. elegans</em>
- name: High-throughput Phenotyping Assay - SORTER
  file: 20221107_High-throughputSorterAssayProtocol.pdf
  group: <em>C. elegans</em> Phenotyping Methods
- name: High-throughput Phenotyping Imager Assay
  file: 20210928_High-throughputPhenotypingImagerAssay.pdf
  group: <em>C. elegans</em> Phenotyping Methods
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