The following technique is used to analyze DNA obtained from cancer biopsies, in which tumor cells are often contaminated with large numbers of admixed stromal cells: (A) Sanger sequencing, (B) Single base primer extension, (C) Pyrosequencing, (D) Amplicon length analysis
Answer is C. Ans. (c) Pyrosequencing(Ref: R 9th/pg 140; 8th/pg 140)Pyrosequencing:Highly sensitive than Sanger sequencing and can detect even 5% mutated DNAFor this reason, it is be used to analyze DNA obtained from cancer biopsies, in which tumor cells are often "contaminated" with large numbers of admixed stromal cells.