All of the following are used in a PCR reaction, EXCEPT: (A) Buffer, (B) ddNTPs, (C) Oligonucleotide Primer pair, (D) Template DNA
Answer is B. Components of PCR 1. Template DNA 2. Pair of DNA Primers 3. Thermostable DNA polymerase 4. dNTPs 5. Buffer with divalent cation ddNTP (dideoxynucleotide triphosphates) Not used in PCR; Used in sanger's chain-termination method