Which of the following enzymes is used in Recombinant DNA research for Homopolymer tailing: (A) Reverse transcriptase, (B) S1 Transferase, (C) Polynucleotide kinase, (D) Terminal transferase
Answer is D. Terminaldeoxynucleotidyltransferase catalyzes the addition of deoxynucleotides to the 3' termini of DNA. ... By use of a tailing method, which adds homopolymerdeoxynucleotide tails to denatured DNA, any double-stranded DNA fragment can be joined to a cloning vehicle Ref biochemistry satyanarayana 4e p255