A patient is having thick, gray coating on the throat and tonsils, followed with fever, chills and swollen glands in the neck. Microscopic examination of nasopharyngeal or pharyngeal swab showed gram positive organism with a special stain. The constitutes of the stain are:-: (A) Crystal violet. Gram's iodine, (B) Toluidine blue, malachite green, glacial acetic, (C) Carbol fuchsin, acid alcohol, and methylene blue, (D) Methylene blue
Answer is B. Gram positive organism causing gray colored coating (pseudomembrane) is Corynebacterium diphtheriae . So the specific stain is Albe stain. Contents of Albe stain:-Stain A- glacial acetic acid, malachite green ,toluidine blueStain B- KI + I2