Which one of the following enzymes is obtained from Thermophillus aquaticus bacterium which is heat stable and used in PCR at high temperature:: (A) DNA polymerase III, (B) Endonuclease, (C) Taq polymerase, (D) DNA gyrase
Answer is C. Taq polymerase Heat stable Derived from a bacteria; Thermophillus aquaticus or Thermus aquaticus. Lacks proof reading. Thermostable DNA Polymerase enzymes with proof reading function in PCR: Pfu: Pyrococcus furiosus Pwo polymerase: Pyrococcus woesei Tli: Thermococcus litoralis - also known as Vent Polymerase Best- Use Pfu in conjunction with Taq polymerase