In estimation of bilirubin, the solution is examined at: (A) 540 nm, (B) 450 nm, (C) 480 nm, (D) 580 nm
Answer is B. Ans: b (450nm) Ref: Vasudevan 4th ed/p.480The characteristic absorption maximums of some importantsubstances are:- Protein, peptide linkage- 220 nmProtein, tryptophan units- 280 nmNucleic acids- 260 nmDeoxy haemoglobin- 565 nmBilirubin- 450 nmNADH- 340 nmFAD- 450 nmFADH2- 570 nmPorphyrin- 400 nm