Oil red o staining used for: (A) Frozen section, (B) Glutaraldehyde fixed specimen, (C) Alcohol fixed specimen, (D) Formalin fixed specimen
Answer is A. Ref Bancroft histology 7/e p 83 Air dry frozen sections on slides for 30 minutes minimum 2. Fix in 10% neutral buffered formalin for 10 minutes 3. Dip in 60% isopropanol 1 time quickly 4. Stain in working Oil Red O solution for 15 minutes 5.