Selective medium for Naegleria fowleri -: (A) Nutrient agar rich with E. coli, (B) NNN media, (C) Non Nutrient agar with E. coli, (D) Diamond media
Answer is C. Option 2 NNN ( Novy-MacNeal-Nicolle) Media is used for culture of Leishmania and Trypanosoma. Option 3 CSF sample can be cultivated on nonnutrient agar (Page's saline and 1.5% agar), lawn cultured with E. coli. Naegleria feeds on bacteria and crawls over the lawn culture of E. coli to produce trails. Option 4 Diamond media (Axenic culture): it is used for isolation of Trichomonas and Entamoeba histolytica.