The following is not a feature of malignant transformation by culturedc: (A) Increased cell density, (B) Increased requirement for growth factors, (C) Alterations of cytoskeleton structure, (D) Loss of anchorage
Answer is B. Ref biology of the cell p357-364 Both normal cells and cancer cells can be cultured in-vitro , however they behave quite differently normal cell: show replicative senescence .is cells pass through limited number of cell division before they decline in vigor and die it may caused by inability to synthesise telomerase