What does the term nick translation refer to?: (A) Translation of RNA into polypeptide, (B) Regulation of gene expression, (C) Technique to label DNA, (D) Technique to digest DNA
Answer is C. A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5' to 3' direction. This propey is used to incorporate radio labeled nucleotides in DNA.