Which of the following is required to bring about gluconeogenesis from pyruvate?: (A) Pyruvate dehydrogenase, (B) Biotin, (C) Alpha ketoglutarate dehydrogenase, (D) Fructose-6 phosphate
Answer is B. Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalysed by a mitochondrial enzyme, pyruvate carboxylase (Fig. 9.24). It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, 7th edition, page no: 118