Isoelectric point of a protein is located by which method?: (A) Isoelectric pointing, (B) Isoelectric cardiac failure, (C) Ion exchange, (D) pH
Answer is B. Ans. is 'b' i.e.,Isoelectric cardiac failure Isoelectric focusing is a procedure used to determine the isoelectric point (pI) of a protein.A pH gradient is established by allowing a mixture of low molecular weight organic acids and bases to distribute themselves in an electric field generated across the gel.When a protein mixture is applied, each protein migrates until it reaches the pH that matches its pI.Proteins with different isoelectric points are thus distributed differently throughout the gel.