In the forensic laboratory some samples are being examined under ultraviolet ray, the doctor notices blue white color of the sample. Which is the most probable content of the sample?: (A) Semen, (B) Urine, (C) Pus, (D) Leucorrhoea discharge
Answer is A. Ans. a. Semen (Ref: Reddy 33/e p434, 29/e p377-378: Parikh 6/e p7.26-7.27)In sexual offences, a preliminary examination under filtered ultraviolet light can be made.Stains of urine, vaginal discharge and seminal fluid show a bright fluorescence.The fluorescence of seminal stains is of a bluish-white colour and such stains are selected for further examinations.Examination of Seminal StainsPhysical Examination:A preliminary examination under filtered ultraviolet lightQ can be made.Stains of urine, vaginal discharge and seminal fluid show a bright fluorescence.The fluorescence of seminal stains is of a bluish-white colour and such stains are selected for further examinationsQ.Chemical Examination: Mnemonic - 'create A F B'Creatine Phosphokinase testQ: Depends on presence of CPKQ in semenAcid Phosphatase testQ: Depends on presence of acid phosphatase in semenFlorence testQ: Depends on presence of CholineQ in semen.Barberio's testQ: Depends on presence of SperminQ in semen.Microscopic examination:Involves identification of spermatozoa under microscope.Fluorescence microscopy is based on the principle that the Y-chromosome is fluorescence to quinacrineQ.Electrophoretic Methods:Acid-Phosphatase TestQLDH Isoenzyme TestQ