Naegler's reaction is used for:: (A) Cl. Tetani, (B) Cl. Botulinum, (C) Cl. Perfringens, (D) Cl. Septicum
Answer is C. Nagler's reaction Nagler's reaction is an example of Neutralization test done for detection of a toxin (lecithinase) produced by C. perfringens. The alpha toxin of C. perfringens has phospholipase activity , when grown on a medium containing egg yolk phospholipid, the organism can break this insoluble triglyceride. Phosphoryl choline release is seen as an area of opacity around the bacterial colonies. Antitoxin can inhibit a positive Nagler's response.