Eosinophilia in necrosed tissue is due to ?: (A) Coagulation of proteins, (B) Denaturation of enzymes, (C) Denaturation of protein, (D) Mitochondrial damage
Answer is C. Necrotic cells show increased eosinophilia in hematoxylin and eosin (H & E) stains. It is mainly due to these 2 Factors Loss of cytoplasmic RNA (or ribonucleoprotein/RNP) which binds the blue dye, hematoxylin. Denatured cytoplasmic proteins which bind the red dye, eosin.