A patient following use of contact lens, developed corneal ulcers and symptoms of conjunctivitis. Saline mount preparation of corneal scrapping shows polygonal cyst. What should be the probable diagnosis?: (A) Acanthamoeba, (B) Naegleria, (C) Entamoeba, (D) Giardia
Answer is A. 1. Acanthamoeba keratitis is associated with corneal injuries complicated by exposure to water or soil and with the wearing of contact lenses. 2. Acanthamoeba keratitis: Risk factors: Extended wear of lens Breaches in hygiene and disinfection procedures Swimming with contact lenses in place Use of homemade saline solutions contaminated with Acanthamoeba. 3. Wet mount examination: CSF or corneal scrapping is done to demonstrate both cysts and trophozoites. Trophozoite: One form, no flagellated form Thorn like pseudopodium (acanthopodia) 15-25 mm size. Giardia and Enthamaeba cause gastroenteritis Nigleria cause fulminant meningitis.