A variety of basic assays are used to detect autoantibodies. Nephelometry is based on the principle of:: (A) Light attenuated in intensity by scattering, (B) Refraction of light, (C) Reduced transmission of light, (D) Filtration of solutes by kidney
Answer is A. Nephelometry measures the interaction of antibodies and antigens in solution, detecting immune complex formation by monitoring changes in the scattering of an incident light. Ref: Imboden J.B. (2007). Chapter 3. Laboratory Diagnosis. In J.B. Imboden, D.B. Hellmann, J.H. Stone (Eds), CURRENT Rheumatology Diagnosis & Treatment, 2e.