A young boy had a flea bite while working in a wheat grain godown. After 5 days he developed fever and had axillary lymphadenopathy. A smear was sent to the laboratory to perform a specific staining. Which one of the following staining method would help in the identification of the suspected pathogen:: (A) Albe staining, (B) Zeihl Neelson staining, (C) Mc Fadyean staining, (D) Wayson staining
Answer is D. The most likely disease to be caused by the flea bite in a young boy is PLAGUE caused by YERSINIA PESTIS and smear is stained with WAYSON stain. Albe staining:- staining the volutin granules found in Corynebacterium diphtheriae Zeihl Neelson staining:-for Mycobacterium Mc Fadyean staining:-M'Fadyean Stain(Polychrome Methylene blue) is used for capsule staining of Bacillus anthracis.