Which test uses oligomer with single base pair substitution as a primer -: (A) PCR, (B) RFLP, (C) Error coded mutation analysis with PCR, (D) Site directed mutagenesis
Answer is D. Site-directed mutagenesis or oligonucleotide-directed mutagenesis uses oligomer with single base pair substitution as a primer to create a specific DNA sequence change (in gene), thus producing modified proteins with a change in primary structure.