For karyotyping, the dividing cells are arrested by the addition of colchicines in the following mitotic phase: (A) Prophase, (B) Metaphase, (C) Anaphase, (D) Telophase
Answer is B. b. Metaphase(Ref: Nelson's 20/e p 605-606, Ghai 8/e p 645)Chromosomes are examined after arresting dividing cells in metaphase with mitotic spindle inhibitors (e.g., N-diacetyl- N-methylcolchicine), followed by staining.