All of the following statements about hybridoma technology are true except -: (A) Specific antibody producing cells are integrated with myeloma cells, (B) Myeloma cells with mutation salvage pathway grows well in HAT medium., (C) Aminopterin, a folate antagonist, inhibits denovo pathway, (D) HGPRT ase and thymidylate synthetase are required for salvage pathway
Answer is B. Myeloma cells lacks HGPRT enzyme, hence they cannot grow well in HAT medium.'
Ref: Apurba Sastry’s Essentials of Medical Microbiology p117