A 45-year-old female complains of lower abdominal pain and vaginal discharge. On examination, there is cervicitis along with a mucopurulent cervical discharge. The best approach to isolate the possible causative agent would be: (A) Culture chocolate agar supplemented with Haemin, (B) Culture on McCoy cells, (C) Culture on a bilayer human blood agar, (D) Culture on vero cell lines
Answer is B. Sexually transmitted diseases may be present in the following ways: Vaginal discharge, Cervical/urethral discharge due to Neisseria gonorrhoea or Chlamydia trachomatis Genital ulcers etc. The culture for sexually transmitted diseases is done by Irradiated McCoy cells medium Ref: Ananthanarayan and Paniker&;s Textbook of Microbiology, 9th edition; Pg no 675