In PCR, DNA polymerase is used in: (A) DNA replication, (B) DNA Elongation, (C) DNA Mulitiplication, (D) All
Answer is C. All PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase (an enzyme originally isolated from the bacterium Thermus aquatics).This DNA polymerase enzymatically assembles a new DNA strand from DNA building blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required for initiation of DNA synthesis.