SYBR Green Dye is used for:: (A) HPLC, (B) Immuno-fluorescence, (C) PCR, (D) ELISA
Answer is C. Dyes used in real time PCR SYBR based detection TaqMan based detection Uses SYBR Green dye ds-DNA binding dye Not sequence specific Uses a fluorogenic probe specific to target gene It accumulates during PCR. Real Time PCR / Quantitative Fluorescence PCR (QF-PCR) / q PCR: Products are detected by fluorescence during (NOT after) the PCR reaction (in real time), not end time PCR.