Best selective media for Salmonella ?: (A) Thiosulphate citrate bile salt agar(TCBS), (B) Wilson and Blair's brilliant-green bismuth sulphite agar medium, (C) Sorbitol MaCconkey agar, (D) All of the above
Answer is B. Wilson and Blair's brilliant-green bismuth sulfite agar (WBBS) -Best selective and indicator medium used for the isolation of Salmonella species. Bismuth sulfite, brilliant green - inhibitors- hinder the growth of most bacteria while allowing salmonella to grow. Ferrous sulphate- Reacts with H2S - formation of ferrous sulfide-brown-black coloration and also diffuses into the surrounding medium, giving a brown -black halo. Option 1: Thiosulphate citrate bile salt agar(TCBS) is used for Vbrio. Option 3: Sorbitol MaCconkey agar -For EHEC (Enterohaemorrhagic Escherichia coli).