For karyotyping, the dividing cells are arrested by the addition of colchicine in which of the following mitotic phase?: (A) Prophase, (B) Metaphase, (C) Anaphase, (D) Telophase
Answer is B. Chromosomes are examined after arresting dividing cells in metaphase with mitotic spindle inhibitors (e.g., N-diacetyl N-methylcolchicine), followed by staining.