Lipid in tissue detected by aEUR': (A) Oil Red 0, (B) Muciramine, (C) PAS, (D) Myeloperoxidase
Answer is A. Oil red 0 Congo red is not used in lipid staining. It is used in the staining of amyloid protein. STANING FOR LIPIDS Non polar lipids Example Stain used * Unconjugated lipids Fatty acids III & IV Oil red 0, Sudan black Sudan Cholesterol Filipin * Esters Cholesteryl esters Schultz Monodi and Tri-glycerides Calcium lipase * Polar lipids Phospholipids Glycerol bases Phosphatidylcholine Nile blue sulfate Phosphatidylserine Nile blue sulfate Phosphatidylethanol amine Plasmal reaction Sphingosine-base Sphingomyelins Fenic hematoxylin Glycolipids Cerebrosides PAS Sulfatides Acetone/Toluidine blue Ganglisides PAS