All the following can be used to detect the mutation, except: (A) Single strand conformational polymorphism, (B) Ligase chain reaction, (C) Polymerase chain reaction, (D) DNA Sequencing
Answer is B. Methods used for detecting mutations 

Cytogenetics analysis  
FISH   
PCR, 
RFLP 
DNA sequencing, Southern blotting 
Microarrays 
Single-stranded conformational polymorphism (SSCP)
RNAase cleavage
Denaturating gradient gel electrophoresis (DGGE)
Protein truncation test