In which of the following oligonucleotide primer is used?: (A) RFLP, (B) PCR, (C) FISH, (D) Chromosomal walking
Answer is B. DNA sequencing is used to determine the nucleotides in a DNA strand. The Sanger chain termination method of sequencing uses a primer to sta the chain reaction. In PCR, primers are used to determine the DNA fragment to be amplified by the PCR process. Ref-Sathyanarayana 4/e p386