Tyndallisation is a method of -: (A) Growing bac teria in pure culture, (B) Sterilization, (C) Inoculation of media, (D) Preserving cultures
Answer is B. A single exposure of 90 minutes usually ensures sterilization but for media containing sugars or gelatin, an exposure of 100-degree Celsius for 20 minutes on 3 successive days is used. This is known as tyndallisation or intermittent sterilization REF:Ananthanarayan and Panicker's Textbook ofMicrobiology 8thEdition pg no:32