BCR-ABL fusion gene is detected by?: (A) Flow cytometry, (B) FISH, (C) Karyotyping, (D) RT-PCR
Answer is B. Ans. (b) FISH(Ref: Robbins 9th/pg 616-618)Flow cytometry: is used for immunophenotyping in Leukemias & LymphomasFISH- used for detecting submicroscopic chromosomal aberrations of BCR-ABL fusionKaryotyping: Used for detecting chromosomal defects of at least 5 mb size (low sensitivity)RT-PCR: used for quantitative DNA estimation