Method used for acid fast staining -: (A) Robertson's method, (B) Ziehl Neelsen, (C) Silver imprignation method, (D) Dark ground illumination
Answer is B. Ans. is 'b' i.e., Ziehl Neelsen Acid fast stainingo After staining with aniline dye, acid fast organisms resist decolourisation with acids,o Method most commonly used is modified Ziehl Neelsen.Smear stained by carbolfuschin|Decolourization by 20% sulphuric acid|Counterstaining by methylene blueAcid fast organismsNon acid fast organismsRetain fiischin (red colour)Take colour of methylene blue (blue colour)Acid fastness depends uponMycolic acid Integrity of cell wall