Which of the following method is used for the analysis of the C-terminal end of a polypeptide?: (A) Sanger's method, (B) Edman's degradation method, (C) Akabori method, (D) None of the above
Answer is C. Akabori method is used for the analysis of C-terminal end of a polypeptide N-terminal analysis Carboxy-terminal analysis For post-translational modifications Sanger's reagent- fluoro-2,4 dinitrobenzene (FDNB) Carboxypeptidase assay Mass spectrometry Edman's reagent- Phenylisothiocyanate (PITC) Akabori reaction