Ketone body that cannot be detected by Rothera's test:: (A) Acetoacetate, (B) Acetone, (C) Beta hydroxy butyrate, (D) All the above can be detected
Answer is C. Rothera's test can only detect acetoacetate and acetone, and not beta-hydroxy butyrate. Rothera's test: Ketone bodies react with sodium nitroprusside (in the presence of NH3) to form a purple colored ring. Positive test indicate Acetoacetate and Acetone. No specific test available for beta-hydroxy butyrate. So, it is first oxidised to Acetoacetate, then Rothera's test is performed.