What is involved in the formation of d-TMP from d-UMP?: (A) N5, N10-methylene tetrahydrofolate, (B) Form imino folate, (C) N5 formyl folate, (D) Dihydro folate
Answer is A. The following reaction is catalyzed by thymidylate synthetase:5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMPBy means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5, N10-methylene tetrahydrofolate together used to form dTMP, yielding dihydrofolate as a secondary product.