Factors affecting sickling in sickle cell anemia are -: (A) HbS concentration, (B) HbA, (C) pH, (D) Oxygenation
Answer is A. Formation of sickle cell depends on HbS concentration since HbS polymerises and grow beyond the RBC membrane ,prject through it. Referance: Exam preparatory manual for UGs -Pathology by Ramdas Nayak Page no:274 Robbins 9/e pg