Radioisotopes are used in the following technique except –: (A) Mass spectroscopy, (B) RIA, (C) ELISA, (D) Sequencing of nucleic acid
Answer is C. ELISA (Enzyme linked Immunoabsorbent Assay) is an enzyme-based immunoassay and does not use radioactive isotopes. A test similar to ELISA which uses radioactive isotope in place of the enzyme is known as Radioimmunoassay (RIA). Nucleic acid sequencing, mass spectroscopy & RIA are tested using radioisotopes.