A young male patient presented to OPD with urethral discharge. On urine examination pus cells were found but not organisms. Which method would be the best for culture: (A) McCoy cell line, (B) Thayer Main medium, (C) PPLO broth, (D) Cooked meat medium
Answer is A. Ans. a. McCoy cell line Urine examination in a patient of urethral discharge demonstrating only pus cells but no gram-negative diplococcic suggest "non-gonococcal urethritis". The most common cause of non-gonococcal urethritis is Chlamydia trachomatis. Chlamydia trachomatis is cultured on McCoy cells, HeLa cells or Hep-2 cells. Culture Media Organism McCoy cell line ChlamydiaQ Thayer Main medium NeisserieQ PPLO broth MycoplasamQ Cooked meat medium Cooked meat mediumQ Organisms causing Non-gonococcal Urethritis Chlamydia trachomatis (MC) Q Gardnerella vaginalisQ Urea plasma urealyticumQ Candida albicans Mycoplasma genitaliumQ Trichomonas vaginalis Herpes virusQ Mechanical or chemical iinitation CMVQ