Which of the following is used for sequencing a long DNA fragment :: (A) Sanger's technique, (B) Chain termination method, (C) Chromosome Walking, (D) RFLP
Answer is C. Chromosome walking Sequencing method of choice for sequencing long DNA fragments between 1.3 and 7 kb Applications of chromosome walking: Positional cloning Find, isolate, and clone a paicular allele Isolate a locus of interest for which no probe is available Analysis of genetically transmitted diseases Detect mutations Study SNPs (Single Nucleotide Polymorphisms) Disadvantage : Speed of this technique can't be enhanced