Composition of ZN stain is all Except: (A) Basic fuschin, (B) Acid fuschin, (C) Phenol, (D) Alcohol
Answer is A. Primary stain : 0.3% Carbol Fuchsin - Dissolve 50g phenol in 100ml ethanol (90%) or methanol(95%). Dissolve 3g basic fuchsin in the mixture and add distilled water to bring the volume to 1 L. Counter stain : 0.3% methylene blue or malachite green Ref ananthnarayana and panikers microbiology textbook 8/e