Intact peptide bond is necessary for which test?: (A) UV diffraction, (B) Ninhydrin, (C) Diazo reaction, (D) All
Answer is B. Ninhydrin, widely used for detecting amino acids, forms a purple product with -amino acids and a yellow product with the imine groups of proline and hydroxyl-proline.Ref: Harper 28th Ed. Page-20, Harper 27th Ed. Page: 20