A young male patient presented with UTI. On urine examination pus cells were found but no organisms. Which method would be best used for culture?: (A) Mc Coy culture, (B) Thayer main medium, (C) L.J. Medium, (D) Levinthal Medium
Answer is A. Urethritis can be broadly divided into gonococcal and non-gonococcal urethritis. Gonococcal urethritis is caused by Neisseria Gonorrhoeae. Absence of pus cells rules out gonococcal infection. Chlamydia is the most common cause of non gonococcal urethritis accounting for 30-40% of cases. Cell culture used to isolate chlamydia are McCoy cells, HeLa-229 cell line, BHK-21 cells or L-929 cells. Ref: Bacterial Infections of Humans: Epidemiology and Control, 3rd edition, Alfred S. Evans, Chapter 10, Page 200; Sexually Transmitted Infections: Diagnosis, Management, and Treatment, Jonathan M. Zenilman, Page 129; Harrison 16th Edition, Page 764; Ananthnarayanan 7th Edition, Page 425.