Carboxylases requires: (A) Vitamin B12, (B) Folic acid, (C) Niacin, (D) Biotin
Answer is D. Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalyzed by a mitochondrial enzyme, pyruvate carboxylase. It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, page no: 103