Staining technique for cryptosporodium: September 2012: (A) Indian ink, (B) Modified acid fast, (C) Giemsa, (D) Methylene blue
Answer is B. Ans. B i.e. Modified acid fast Staining of cryptosporidium Traditional parasitology stains (e.g., Giemsa) are of limited value. They do not differentiate between oocysts and similarly-sized fecal yeasts (the main differential diagnosis of Cryptosporidium in microscopy) and other debris. Modified acid-fast staining technique is a simple and effective method: the oocysts stain bright red against a background of blue-green fecal debris and yeasts. The acid-fast staining technique has been modified and improved, including: hot and cold modified acid-fast stains; incorporation of dimethyl sulfoxide (DMSO); and incorporation of the detergent tergitol.