All are added to PCR, except:: (A) Deoxynucleotide, (B) Dideoxynucleotide, (C) Thermostat DNAP, (D) Template DNA
Answer is B. Ans. B. Dideoxynucleotide(Ref: Tietz Textbook of Clinical Chemistry 4fe page1446)Pre requisites of PCRSample DNA to be amplifiedDeoxynucleotides.Thermostable Polymerase--Taq Polymerase obtained from Thermus Aquaticus found in hot springs.Primer.Mgcl2 , KCl