The rate of newly synthesized Osteoid mineralization is best estimated by: (A) Tetracycline labelling, (B) Alizarine red staining, (C) Calceine stain, (D) Von Kossa stain
Answer is A. Reference Robbins page no 358 6th edition Tetracycline is absorbed into bone and so it is used as a marker of bone growth for biopsies in humans. Tetracycline binds to newly formed bone at the bone interface where it shows as a linear fluroscencse. Tetracycline labelling is used to determine the amount of bone growth within a ceain period of time