Labile toxin of E.coli can be detected by the following methods of incubation except ?: (A) Into infant rabbit bowel, (B) Into adult rabbit skin, (C) Infra gastrically into infant Mouse, (D) Into Tissue Culture of Chinese Hamster ovary cells
Answer is C. Ans. is 'c' i.e., lntra gastrically into infant mouse Methods for detection of ETEC enterotoxins Assay LT ST In vivo tests +- + + ? Ligated rabbit deal loop Read at 6 hours Read at 18 hours Infant rabbit bowel + + Infant mouse intragastric (4 hours) - + Adult rabbit skin (vascular permeability) + ? In vitro tests Tissue culture tests Rounding of Y1 mouse adrenal cells Elongation of chinese hamster ovary (CHO) cells + ? Serological tests ELISA + (ST-ELISA with monocolonial antibody) Passive agglutination tests, passive immune - hemolysis, precipitin (Eiken's) test + Genetic tests + DNA probes +