An index of the binding affinity of a hormone for its receptor can be obtained by examining the: (A) Y-intercept of a Scatchard plot, (B) Slope of a Scatchard plot, (C) Maximum point on a biological dose-response curve, (D) X-intercept of a Scatchard plot
Answer is B. Scatchard plots of hormone-receptor binding data give information regarding the number of receptors and the affinity of the hormone for its receptor. The x-intercept provides data regarding total receptor number, and the slope is equal to the negative of the association constant (-Ka).