size = screen.GetSize()
bmp = wx.EmptyBitmap(size[0], size[1])
mem = wx.MemoryDC(bmp)
mem.Blit(0, 0, size[0], size[1], screen, 0, 0)
del mem
bmp.SaveFile('screenshot.png', wx.BITMAP_TYPE_PNG)
Get screenshot on Windows with Python?