s2 = (
    '53.80  64-66-04.630N  52-16-15.355W  25-JUN-1993:16:48:34.00  S10293..  2'
    )

def spaceTest(line):
    matches = re.findall('\\s+', line.strip())
return not any(m for m in matches if m != '  ')
print(spaceTest(s))
print(spaceTest(s2))
Finding double-spaces in a string - Python