This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pathology
Eosinophilia in necrosed tissue is due to ? 
Select and explain. 
A. Coagulation of proteins, B. Denaturation of enzymes, C. Denaturation of protein, D. Mitochondrial damage
C: Denaturation of protein
Answer: C: Denaturation of protein 
Explanation: Necrotic cells show increased eosinophilia in hematoxylin and eosin (H & E) stains. It is mainly due to these 2 Factors Loss of cytoplasmic RNA (or ribonucleoprotein/RNP) which binds the blue dye, hematoxylin. Denatured cytoplasmic proteins which bind the red dye, eosin.