This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Replication, Subject name: Biochemistry
Primers are removed by all except: 
Select and explain. 
A. Delta Polymerase, B. RNase H1, C. FEN1, D. None
D: None
Answer: D: None 
Explanation: Primers are synthesized during DNA replication. Primer is RNA primer and RNA should not be there as a component of DNA So at the completion of replication we have to remove this RNA primer then we have to keep DNA instead of it. Enzyme of removal of primer in prokaryotes are - DNA polymerase I In Eukaryotes There are 2 organelles where DNA is present Nuclease and mitochondria Enzymes responsible for primer removal in nuclease are - RNase H - FEN-1 and - d polymerase (minor), Enzymes responsible for primer removal in mitochondria are RNase H and FEN-1