This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General anatomy, Subject name: Anatomy
The following is not a feature of malignant transformation by culturedc 
Select and explain. 
A. Increased cell density, B. Increased requirement for growth factors, C. Alterations of cytoskeleton structure, D. Loss of anchorage
B: Increased requirement for growth factors
Answer: B: Increased requirement for growth factors 
Explanation: Ref biology of the cell p357-364 Both normal cells and cancer cells can be cultured in-vitro , however they behave quite differently normal cell: show replicative senescence .is cells pass through limited number of cell division before they decline in vigor and die it may caused by inability to synthesise telomerase