This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: general microbiology, Subject name: Microbiology
NNN media used in? 
Select and explain. 
A. Leishmania, B. Histoplasma, C. Trypanosoma, D. Entamoeba
A: Leishmania
Answer: A: Leishmania 
Explanation: Diagnosis Novy-Macneal-Nicole Medium Laboratory diagnosis of leishmaniasis can include the following: Isolation, visualization, and culturing of the parasite from infected tissue Serologic detection of antibodies to recombinant K39 antigen Polymerase chain reaction (PCR) assay for sensitive, rapid diagnosis of Leishmania species Other tests that may be considered include the following: CBC count, coagulation studies, liver function tests, peripheral blood smear Measurements of lipase, amylase, gamma globulin, and albumin Leishmanin (Montenegro) skin testing (LST) (not FDA approved in the United States) Novy-MacNeal-Nicolle medium (NNN) is a culture medium used to grow Leishmania It consists of 0.6% sodium chloride (NaCl) added to a simple blood agar slope. NNN can also be used to grow Trypanosoma cruzi. REF:Medscape