This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Structure and function of protein, Subject name: Biochemistry
Which of the following enzymes, requires Biotin as the coenzyme 
Select and explain. 
A. Pyruvate Dehydrogenase, B. Pyruvate Kinase, C. Pyruvate Carboxylase, D. PEP Carboxy Kinase
C: Pyruvate Carboxylase
Answer: C: Pyruvate Carboxylase 
Explanation: Pyruvate enters the mitochondrion and is carboxylated to oxaloacetate by pyruvate carboxylase, an ATP-dependent reaction in which the coenzyme is the Vitamin biotin.Ref: Harper&;s Biochemistry; 30th edition; Chapter 16; The Citric Acid Cycle: The Central Pathway of Carbohydrate, Lipid & Amino Acid Metabolism