This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: parasitology, Subject name: Microbiology
Acute Primary Amoebic meningoencephalitis true is - 
Select and explain. 
A. Meningitis caused by acanthamoeba species is acute in nature, B. Diagnosis is by demonstration of trophozoite in CSF, C. Caused by feco-oral transmission, D. More common in tropical climate
B: Diagnosis is by demonstration of trophozoite in CSF
Answer: B: Diagnosis is by demonstration of trophozoite in CSF 
Explanation: Wet mount preparation of CSF is examined under microscope for demonstration of motile amoeboid trophozoites with characteristics morphology.Staining of CSF with Wright or Giemsa stains may also be done (refer pgno:26 baveja 3 rd edition)