This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
What does the term nick translation refer to? 
Select and explain. 
A. Translation of RNA into polypeptide, B. Regulation of gene expression, C. Technique to label DNA, D. Technique to digest DNA
C: Technique to label DNA
Answer: C: Technique to label DNA 
Explanation: A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5' to 3' direction. This propey is used to incorporate radio labeled nucleotides in DNA.