This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Bio-Chemistry), Subject name: Biochemistry
Chromatography technique works on the principle of 
Select and explain. 
A. Affinity, B. Ion-exchange, C. Molecular sieving, D. All of the above
D: All of the above
Answer: D: All of the above 
Explanation: (D) All of the above # CHROMATOGRAPHY usually consists of a mobile phase and a stationary phase. The mobile phase refers to the mixture of substances (to be separated), dissolved in a liquid or a gas.> Stationary phase is a porous solid matrix through which the sample contained in the mobile phase percolates.> Interaction between the mobile and stationary phases results in the separation of the compounds from the mixture.> These interactions include the physico-chemical principles such as adsorption, partition, ion-exchange, molecular sieving and affinity.