This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: G.I.T, Subject name: Pathology
In Hirschsprung'S disease, staining used for diagnosis is? 
Select and explain. 
A. Fontana stain, B. Trinchome stain, C. AChE, D. Auramine Rhodamine stain
C: AChE
Answer: C: AChE 
Explanation: Histochemical staining for acetylcholine esterase activity provides confirmation for identifying ganglion cells and nerve trunks. TEXTBOOK OF PATHOLOGY 6TH EDITION HARSH MOHAN PAGE NO:580