This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
Steps of PCR in sequence are ? 
Select and explain. 
A. Extend DNA, Anneal Primers, Denature DNA, B. Anneal Primers, Extend DNA, Denature DNA, C. Denature DNA, Anneal Primers, Extend DNA, D. Denature DNA, Extend DNA, Anneal Primers
C: Denature DNA, Anneal Primers, Extend DNA
Answer: C: Denature DNA, Anneal Primers, Extend DNA 
Explanation: PCR - Method of amplifying a target sequence of DNA developed by Karry Mullis in 1984 Essential requirements of a PCR reaction: Targeted DNA Two primers Four deoxyribonucleotide Thermostable DNA polymerase Steps of PCR: 1. Denaturation: Two DNA strands separated (94o C) 2. Annealing: Addition of primers (one for each strand) (60oC) 3. Extension/Elongation: Polymerization or synthesis of DNA. (72oC) These cycles of Denaturation, Annealing, and Extension are repeated again and again to synthesize large amounts of DNA.