This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Cellular Pathology, Subject name: Pathology
Best method for HbA1c estimation is? 
Select and explain. 
A. Affinity chromatography, B. Ion exchange chromatography, C. Electrophoresis, D. HPLC
D: HPLC
Answer: D: HPLC 
Explanation: Ans. (d) HPLCFour basic types of methods are used most commonly to measure HbA1c: immunoassay, ion-exchange high- performance liquid chromatography (HPLC), boronate affinity HPLC, and enzymatic assays. The gold standard is HPLC method