This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: DNB 2018, Subject name: Biochemistry
Hybridoma used in: 
Select and explain. 
A. In situ hybridization, B. Sequencing DNA, C. Production of monoclonal antibodies, D. Production of continuous cell lines
C: Production of monoclonal antibodies
Answer: C: Production of monoclonal antibodies 
Explanation: ln 1975, George Kohler and Cesar Milstein created hybrid cells that will make unlimited quantities of antibodies with defined specificities, which are termed as monoclonal antibodies . This discovery know as hybridoma technology Principle - fusion between myeloma cell (malignant plasma cell) and spleen cell from a immunized animal. Hybridoma technology can make available highly specific antibodies in abundant amounts. use of monoclonal antibodies Example of monoclonal ab trastuzumab - EGRF/HER2 INHIBITORS BEVACIZUMAB - ANGIOGENESIS INHIBITORS