This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Ophthalmology
Acanthamoeba Keratitis, not true statment is 
Select and explain. 
A. Common in contact lens users, B. Ring abscess seen, C. Pain is very severe, D. Cultured on Nutrient agar enriched with E.coli
D: Cultured on Nutrient agar enriched with E.coli
Answer: D: Cultured on Nutrient agar enriched with E.coli 
Explanation: Acanthamoeba cultured on non-nutrient agar enriched with E.coli.