This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General, Subject name: Microbiology
Sterilisation of media containing serum: 
Select and explain. 
A. Autoclaving, B. Micropore filter, C. Gamma radiation, D. Centrifugation
B: Micropore filter
Answer: B: Micropore filter 
Explanation: Ans. is 'b' i.e., Micropore filterSerum, trypsin, growth factors, proteins, amino acids, vitamins and hormones are thermolabile and may decompose during autoclaving, so must be sterilized by Membrane filters.Membrane filters has less pore size than bacteria size and made up with cellulose acetate, cellulose nitrate, polycarbonate etc.