This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Hemolytic Anemias, Subject name: Pediatrics
6 months old infant with Hb of 6 gm% has anaemic features with peripheral blood showing target cells. Family history of similar complaints is (Positive). Diagnosis can be best done with which of the following: 
Select and explain. 
A. Hb electrophoresis, B. ELISA, C. Coomb's Test, D. Osmotic fragility
A: Hb electrophoresis
Answer: A: Hb electrophoresis 
Explanation: Ans. is 'a' i.e. Hb Electrophoresis (Ref. Harrison, 16/e, 595 (15/e, p. 681), Nelson 17/e, p 1630-1632).Presence of:Anaemia (Hb < 6%)Target cellsIncrease reticulocytes suggests the diagnosis of thalassemia.Thalassemia is a hemoglobin disorder Hemoglobin disorders are diagnosed by electrophoresis.Diagnosis of thalassemia is confirmed by serum electrophoresis.The finding is- Increase Hb A2*Increase Hb F*Other conditions in which Target cells are seenLiver disease*Hemoglobin C and S disease*