This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Bacteriology, Subject name: Microbiology
Diene method is used for 
Select and explain. 
A. Mycoplasma, B. Chlamydia, C. Plague, D. Diphtheria
A: Mycoplasma
Answer: A: Mycoplasma 
Explanation: Mycoplasma colonies may be seen with a hand lens but are best studied after staining by the Dienes method. For this, a block of agar containing the colony is cut and placed on a slide. It is covered with a coverslip on which an alcoholic solution of methylene blue and azure has been dried. Ref: Textbook of microbiology; Ananthanarayan paniker's; 10th edition; Pg:394