This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Structure & Function of Protein, Subject name: Biochemistry
Proteins can be separated by the following methods except: 
Select and explain. 
A. Electrophoresis, B. Ultra-centrifugation, C. Gas-liquid Chromatography, D. Salt separation
C: Gas-liquid Chromatography
Answer: C: Gas-liquid Chromatography 
Explanation: Ans. C. Gas-liquid ChromatographySeparation of Plasma proteins by:a) Salting out method (using ammonium sulphate)Three proteins are separated- Albumins- Full Saturation- Globulins-Half Saturationb) Gas Liquid chromatography is used to separate volatile substances on heating. Therefore not used to separate proteins, because they get coagulated or denatured