This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Virus can be isolated from clinical samples by cultivation in the following except: 
Select and explain. 
A. Tissue culture, B. Embryonated eggs, C. Animals, D. Chemically defined media
D: Chemically defined media
Answer: D: Chemically defined media 
Explanation: Ans. (d) Chemically defined media Ref Ananthanarayan 8/e 450, 9/e, p 451 Isolation of virus Method of isolation consists of: "Inoculation into animals, eggs or tissue culture after the processed to remove bacterial contaminants." Ananthanarayan 8/e, p 450, 9/e, p 451 As many viruses (adenoviruses, enteroviruses) are frequently found in normal individuals, so only recovery of viral agent from patient doesn't prove that it is the causative agent of the patient illness. Organism not grown in aificial cultural media are: * Chlamydia * Rickettsia Rhinosporidium * Pathogenic treponemes * M. leprae Pneumocystis * Viruses.