This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Kelly's medium is used in the isolation of ? 
Select and explain. 
A. Leptospira, B. Borrelia, C. Baonella, D. Brucella
B: Borrelia
Answer: B: Borrelia 
Explanation: Ans. is 'b' i.e., Borrelia . Modified Kelley's (BSK) medium is used for Borrelia burgdorferi. Laboratory diagnosis of Lyme disease . The culture of B. burgdorferi in Barbour - Stoenner - kelly (BSK) medium permits definitive diagnosis, but this complex method has been used only research studies. . Because of the problems associated with direct detection of B. burgdorferi, lyme disease is usually diagnosed by the recognition of a characteristic clinical picture with serological confirmation. . A two step approch is used in which samples are first tested by ELISA and equivocal or positive results are then tested by western blotting. . Major use for PCR testing in lyme disease --> Detection of B. burgdorferi in synol fluid.