This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
An amoebic liver abscess can be diagnosed by demonstrating- 
Select and explain. 
A. Cysts in the sterile pus, B. Trophozoites in the pus, C. Cysts in the intestine, D. Trophozoites in the feces
B: Trophozoites in the pus
Answer: B: Trophozoites in the pus 
Explanation: Exploratory puncture is one of the most practical methods for confirming the diagnosis of amboebic liver abscess. The aspirated pus may be examined for the demonstration of trophic forms (trophozoites) of E. histolytica.
Aspirations from the center of amoebic liver abscess do not show trophozoites, while aspirates from the margins show trophozoites

About other options
Option 'a'
"Cysts are never seen in extraintestinal lesions"
Option 'c & d'

In extraintestinal amoebiasis, often stool examination is negative