This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pathology
The following is not a feature of malignant transformation by cultured cells - 
Select and explain. 
A. Increased cell density, B. Increased requirement for growth factors, C. Alterations of cytoskeletal structures, D. Loss of anchorage
B: Increased requirement for growth factors
Answer: B: Increased requirement for growth factors 
Explanation: Cultured cells undergoing malignant transformation do not show increased requirement of growth factors. On the contrary they lose the requirement of growth factors.