This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General Microbiology Pa-2 and Pa-3 (Sterilization and Bacterial Genetics), Subject name: Microbiology
Hot air oven is used for sterilization of all except - 
Select and explain. 
A. Glass wares, B. Dusting Powder, C. L.J.media, D. Liquid paraffin
C: L.J.media
Answer: C: L.J.media 
Explanation: Hot air oven - most widely used method of sterilization by dry heat Temperature for sterilization - 160degC for 2 hours Control used - spores of Bacillus subtilis Materials sterilized - 1) Glassware like glass syringes, petri dishes, flasks, pipettes & test tubes 2) Surgical instruments like scalpels, forceps etc. 3) Chemicals such as liquid paraffin, fats, glycerol etc. Culture Media are sterlized by Autoclaving.