This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
All the following can be used to detect mutation, except: 
Select and explain. 
A. Single strand conformational polymorphism, B. Ligase chain reaction, C. Polymerase chain reaction, D. DNA Sequencing
B: Ligase chain reaction
Answer: B: Ligase chain reaction 
Explanation: B i.e. Ligase chain reactionMutations can be detected by DNA sequencing PCR, PCR, single strand conformational polymorphism (SSCP), RFLP, and denaturing gradient gel electrophoresisQ.