This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Medicine
A 5 year old girl presents with pallor, abdominal examination shows hepatosplenomegaly. Which of the following test is most appropriate in making the diagnosis? 
Select and explain. 
A. Osmotic Fragility Test, B. Hb Electrophoresis, C. Peripheral Smear Examinations, D. Examination of Bone Marrow
B: Hb Electrophoresis
Answer: B: Hb Electrophoresis 
Explanation: The child is showing features of beta thalssemia intermedia. Definitive diagnosis of this condition can be made by hemoglobin electrophoresis which shows HbF in the range of 10-50%, High HbA2 >4%. Criteria for diagnosing beta thalassemia intermedia includes: Clinical: Late presentation 2-5years, with relatively high hemoglobin 8 -10 g/dl and moderate bone changes and hepatosplenomegaly. Serum electrophoresis showing HbF in the range of 10-50%, High HbA2 >4% Genetics: One or both parents can be a silent carrier, absence of heterozygosity for beta thalassemia or presence of high HbF beta thalassemia. Molecular: Mild beta thalassemia mutation, Co inheritance of alpha thalassemia, co inheritance of high fetal hemoglobin. Ref: Harrison's Internal Medicine, 18th Edition, Page 859 ; Kochars Clinical Medicine for Students By Dario.M.Torre, Page 582