This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: miscellaneous, Subject name: Biochemistry
Rothera&;s test used for detection of 
Select and explain. 
A. Proteins, B. Glucose, C. Fatty acid, D. Ketones
D: Ketones
Answer: D: Ketones 
Explanation: The presence of ketosis can be established by the detection of ketone bodies in urine by Rothera&;s test.Rothera&;s test: Saturate 5 mL of urine with solid ammonium sulfate. Add a few drops of freshly prepared sodium nitroprusside followed by 2 mL of liquor ammonia along the sides of the test tube. Development of a purple ring indicates the presence of ketone bodies in urine. Strip tests based on the same principle are also available.Ref: D M Vasudevan 7th edition Page no: 166