This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
What is meant by melting of double stranded DNA: 
Select and explain. 
A. Splitting of double strands into single strand, B. Splitting of DNA into fragments, C. Formation of triple helix, D. Separation of bases.
A: Splitting of double strands into single strand
Answer: A: Splitting of double strands into single strand 
Explanation: A i.e. Splitting of double strands into single strand.Seperation of double stranded (ds) DNA into single stranded DNAQ is known as denaturation or melting of DNA. It is promoted by increasing temperature or decreasing salt concentration. So increased salt (monovalent cation) concentration increases TmQ.