This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Systemic Bacteriology (Gram Positive Bacilli, Gram Negative Bacilli), Subject name: Microbiology
Shigellosis is best diagnosed by:- 
Select and explain. 
A. Stool examination & culture, B. Serology, C. Sigmoidoscopy, D. Enzyme
A: Stool examination & culture
Answer: A: Stool examination & culture 
Explanation: Shigellosis is best diagnosed by Stool examination & culture. Option 1 1. Stool is the specimen of choice. Diagnosis of shigellosis is made by isolating Shigella spp. from feces. Fresh feces are inoculated without delay or transpoed in a suitable medium, such as Sachs' buffered glycerol saline. Also, rectal swabs may be taken from the site of ulcer by sigmoidoscopy. Option 3 - Sigmoidoscopy: It can reveal the extent of the disease. Option 4 - For diagnosis of Shigellosis: we don't detect any enzyme.