This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Among the following methods, the one that is MOST sensitive for glucose estimation is: 
Select and explain. 
A. GOD POD method, B. O-Toluidine method, C. Folin-Wu method, D. Hexokinase method
A: GOD POD method
Answer: A: GOD POD method 
Explanation: Enzymatic method of glucose estimation is highly specific, giving 'true glucose' values. The glucose oxidase (GOD) and peroxidase (POD) method is the one most widely used. Glucose oxidase is very specific; it conves glucose to gluconic acid and hydrogen peroxide. Peroxidase conves the H2O2 into H2O and nascent oxygen. The oxygen oxidises a colourless chromogenic substrate (e.g., oho dianisidine) to a coloured one; the colour intensity is directly propoional to concentration of glucose. Ref: Textbook of biochemistry by D M Vasudevan, 5th edition, Page 104.