This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Mendalian Inheritance, Subject name: Biochemistry
Which of the following test is not used in epigenetics: 
Select and explain. 
A. HPLC, B. Chip on chip, C. Bi-sulfate sequencing, D. Methylation sensitive Restriction Enzymes digestion
A: HPLC
Answer: A: HPLC 
Explanation: HPLC is not used in epigenetics Tests done in epigenetics are : Na bisulfite method - detects DNA methylation. ChIP- Chromatin Immuno Precipitation- Detects post translational modifications of histones Used together with its large-scale variants ChIP-on-chip and ChIP-Seq FISH (Fluorescent in situ hybridization) Methylation-sensitive restriction enzymes PCR Microarray