This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Diene’s method is used for : 
Select and explain. 
A. Mycoplasma, B. Chlamydiae, C. Plague, D. Diptheria
A: Mycoplasma
Answer: A: Mycoplasma 
Explanation: • Colonies of mycoplasma are typically biphasic, with a fried egg appearance. 
• Colonies are best studied after staining by Dienes method .