This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Musculoskeletal system, Subject name: Anatomy
The rate of newly synthesized Osteoid mineralization is best estimated by 
Select and explain. 
A. Tetracycline labelling, B. Alizarine red staining, C. Calceine stain, D. Von Kossa stain
A: Tetracycline labelling
Answer: A: Tetracycline labelling 
Explanation: Reference Robbins page no 358 6th edition Tetracycline is absorbed into bone and so it is used as a marker of bone growth for biopsies in humans. Tetracycline binds to newly formed bone at the bone interface where it shows as a linear fluroscencse. Tetracycline labelling is used to determine the amount of bone growth within a ceain period of time