This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pharmacology
All of the following factors tend to increase the volume of distribution of a drug EXCEPT: 
Select and explain. 
A. High plasma protein binding, B. Low ionization at physiological pH values, C. High lipid solubility, D. High tissue binding
A: High plasma protein binding
Answer: A: High plasma protein binding 
Explanation: - Factors affecting drug distribution

   Lipid solubility - Higher the solubility, higher the distribution
   Ionization at physiological pH - Higher the ionization, lower the distribution
   Degree of plasma protein binding - More the binding, less the distribution
   Affinity for different tissues - Higher affinity, higher distribution
   Regional blood flow - More the flow, more the distribution
   Fat: lean body mass ratio (changes with age) - Increases with age 
   Disease like CHF, uremia, cirrhosis