This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Bacteria, Subject name: Microbiology
The most sensitive method for detecting cervical Chlamydia trachomatis infection is: 
Select and explain. 
A. Direct fluorescent antibody test, B. Enzyme immunoassay, C. Polymerase chain reaction, D. Culture on irradiated McConkey cells
C: Polymerase chain reaction
Answer: C: Polymerase chain reaction 
Explanation: Ans. is 'c' i.e., Polymerase chain reactionRef: Harrison, 17th/e, p. 1074,19th/e, p. 1165 and Ananthanarayan, 9th/e, p. 415Amplification assays such as ligase chain reaction and polymerase chain reaction are the most sensitive chlamydial diagnostic method.Other methods are:(a) Cell culture: Sensitivity (60-80%).(b) Direct immunofluorescent antibody test: Sensitivity 70-80% and quite specific.(c) ELISA: 60-80%.