This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Which of the following estimates blood creatinine level most accurately - 
Select and explain. 
A. Jaffe method, B. Kinetic jaffe method, C. Technicon method, D. Enzyme assay
D: Enzyme assay
Answer: D: Enzyme assay 
Explanation: Among the given options, the enzyme assay is the most accurate.
Methods to measure serum creatine to assess GFR are:-

i) Gas chromatography-isotope dilution mass spectroscopy (GC-IDMS):- It is the most accurate and method of choice.
ii) High-performance liquid chromatography (HPLC).
(iii) Enzymatic creatinine method (enzyme assay).
(iv)Alkaline picrate methods:- Jaffe method

Accuracy in decreasing order is GC-IDMS > HPLC > Enzyme assay > Alkaline picrate (Jaffe) method.