This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Acute Primary Amoebic meningoencephalitis true is- 
Select and explain. 
A. Meningitis caused by acanthamoeba species is acute in nature, B. Diagnosis is by demonstration of trophozoite in CSF, C. Caused by faeco-oral transmission, D. More common in tropical climate
B: Diagnosis is by demonstration of trophozoite in CSF
Answer: B: Diagnosis is by demonstration of trophozoite in CSF 
Explanation: Diagnosis of acute primary amoebic meningoencephalitis is established by microscopic examination of the cerebrospinal fluid which contains trophozoite and red cells but no bacteria.

About other options

Acanthamoeba causes chronic meningoencephalitis, while Naegleria fowleri is responsible for acute cases of meningoencephalitis.
The cases are seen in children who swim or dive in warm, soil contaminated pools (in temperate regions e.g. in Europe and N. America).
The amoebas enter via the nose and the cribriform plate of the ethmoid passing directly into the brain tissue where they form nests of amoebas that cause extensive hemorrhage and damage, chiefly in the basilar portion of the cerebrum and cerebellum.