This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Genetic and genetic disorders, Subject name: Pediatrics
Which of the following is used in gene therapy? 
Select and explain. 
A. Electroporation, B. Electrofocusing, C. Selectively targeted recombination, D. Intracytoplasmic injection
A: Electroporation
Answer: A: Electroporation 
Explanation: Electroporation is used in gene therapy. Gene therapy is a technique that uses genes to treat or prevent disease. Delivery of DNA into patient&;s cells by using recombinant viruses/naked DNA/DNA complexes. Methods used include intranuclear injection, electroporation, gene-gun, sonoporation, and magnetofection Gene therapy was first used for severe combined immunodeficiency disorder (SCID), which is due to adenosine deaminase enzyme deficiency. FDA-approved gene therapy is used for SCID, Leber&;s congenital amaurosis, multiple myeloma and adrenoleukodystrophy Ref: Nelson textbook of pediatrics 21st edition Pgno: 584-586