This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
All the following can be used to detect the mutation, except 
Select and explain. 
A. Single strand conformational polymorphism, B. Ligase chain reaction, C. Polymerase chain reaction, D. DNA Sequencing
B: Ligase chain reaction
Answer: B: Ligase chain reaction 
Explanation: Methods used for detecting mutations 

Cytogenetics analysis  
FISH   
PCR, 
RFLP 
DNA sequencing, Southern blotting 
Microarrays 
Single-stranded conformational polymorphism (SSCP)
RNAase cleavage
Denaturating gradient gel electrophoresis (DGGE)
Protein truncation test