This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Parasitology Pa 1 (Protozoology), Subject name: Microbiology
Acute primary meningoencephalitis true is - 
Select and explain. 
A. Meningitis caused by Acanthamoeba species is acute in nature, B. Diagnosis is done by demonstration of trophozoites in CSF, C. Caused by feco-oral transmission, D. More common in tropical climate
B: Diagnosis is done by demonstration of trophozoites in CSF
Answer: B: Diagnosis is done by demonstration of trophozoites in CSF 
Explanation: Option 1, 2,3 Naegleria Fowleri: Disease: Primary amebic meningoencephalitis (PAM). Infective form: Amoeboid form is the invasive form. Mode of transmission: Man acquire infection by nasal contamination during swimming in fresh hot water bodies like ponds, river, swimming pools or lakes. option 4 Acanthamoeba species are more common in tropical climate