This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Bio-Chemistry), Subject name: Biochemistry
In estimation of bilirubin, the solution is examined at 
Select and explain. 
A. 540 nm, B. 450 nm, C. 480 nm, D. 580 nm
B: 450 nm
Answer: B: 450 nm 
Explanation: Ans: b (450nm) Ref: Vasudevan 4th ed/p.480The characteristic absorption maximums of some importantsubstances are:- Protein, peptide linkage- 220 nmProtein, tryptophan units- 280 nmNucleic acids- 260 nmDeoxy haemoglobin- 565 nmBilirubin- 450 nmNADH- 340 nmFAD- 450 nmFADH2- 570 nmPorphyrin- 400 nm