This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pathology
Investigation of choice to confirm sickle cell disease 
Select and explain. 
A. Tandem mass spechrometry, B. Gel Electrophoresis, C. High performance liquid chromatography, D. Naked eye single Tube Red Cell Osmotic Fragility test
C: High performance liquid chromatography
Answer: C: High performance liquid chromatography 
Explanation: HPLC is the investigation of choice to confirm the diagnosis of sickle cell anemia.