This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: general microbiology, Subject name: Microbiology
In &;Hotair oven&; for holding period of one hour, temperature required is - 
Select and explain. 
A. 100o C, B. 120o C, C. 140o C, D. 160 o C
D: 160 o C
Answer: D: 160 o C 
Explanation: Temperature and time: 160 o C for 2 hours (holding time ) is required for sterilization However alternative temperatures and holding time include 170degC for 1 hour and 180degC for 30 minutes Ref: a textbook of Microbiology Dr.C.P.Baveja 4th Edition pg no:30