This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Forensic Medicine
Which of the following test detects spermine in semen during examination of seminal fluid in a case of sexual assault? 
Select and explain. 
A. Barberio test, B. Florence test, C. ELISA, D. Gettler's test
A: Barberio test
Answer: A: Barberio test 
Explanation: Barberio test detects spermine in semen. A saturated aqueous or alcoholic solution of picric acid when added to spermatic fluid produces yellow needle-shaped rhombic crystals of spermine picrate. The reaction depends on the presence of prostatic secretion. Ref: Essentials of Forensic Medicine and Toxicology by KS Narayan Reddy, 27th edition, Page 370.