This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Unknown
During staining for Electron Microscopy, the method which improves contrast of specimen is 
Select and explain. 
A. Positive staining, B. Negative staining, C. Shadow staining, D. None of these
B: Negative staining
Answer: B: Negative staining 
Explanation: The specimen stained with phosphotungstic acid in electron microscope for better visualisation of the structures. Negative staining with phosphotungstic acid used in electron microscope.