This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Technique (s) used to detect Gene Mutation is/are 
Select and explain. 
A. #NAME?, B. Denaturing gradient gel electrophoresis, C. DNA sequencing and Restriction fragment polymorphism (RFLP) both, D. All
D: All
Answer: D: All 
Explanation: ALL i.e. (-PCR, Denaturing gradient gel electrophoresis, DNA sequencing, Restriction fragment polymorphism (RFLP), Single-strand conformational polymorphism