This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: All India exam, Subject name: Microbiology
The most sensitive method to detect LD bodies is 
Select and explain. 
A. Bone marrow aspiration, B. Splenic aspiration, C. Peripheral blood smear, D. Lymph node biopsy
B: Splenic aspiration
Answer: B: Splenic aspiration 
Explanation: Splenic aspiration is the most sensitive method to detect LD bodies (98% positivity). Bone marrow aspiration and biopsy are positive in over 85% of cases. Lymph node aspiration is positive in 60% of cases of African kala-azar but not in cases of Indian kala-azar.