This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: general microbiology, Subject name: Microbiology
Electron microscopy is used for the following except- 
Select and explain. 
A. To differentiate T & B lymphocytes, B. IgG deposits in kidney, C. TPI, D. Flagella
C: TPI
Answer: C: TPI 
Explanation: The scanning Electron microscopy is a recent development which provides a higher resolution and 3 dimensional image of the object . Although the substantial increses in resolution power of Electron microscopy has led to significant discoveries a major disadvantage is the inability to examine living cells A method involves rapid cooling of specimens by deep freezing in liquid gas and the subsequent formation of carbon platinum replicas of the material . Since such frozen cells may remains ble it enables the study of cellular ultra structures as it appears in the living state . Electron microscopy is especially used for rapid detection of viruses in clinical samples . it is paicularly useful for detecting non cultivable viruses . ultrastrucural study of various micro organisms may also be done by this method Ref:textbook of Microbiology Dr.C.P.Baveja 4th Edition pg no: 11