This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Beta thalasemmia, Subject name: Pathology
Diagnosis of thalassaemia major is based on the detection of which of following hemoglobins? 
Select and explain. 
A. HbA, B. HbA2, C. HbF, D. HbS
C: HbF
Answer: C: HbF 
Explanation: The major red cell hemoglobin in thalassemia major is HbF (a2 g2), which is markedly elevated. Thalassemia minor/ trait is associated with increased levels of HbA2( a2 d2 ) HbA (a2 b2) accounts 95% to 98% of the total hemoglobin in normal adults. HbS is seen in sickle cell anemia. These hemoglobins can be detected by Hb high performance liquid chromatography (HPLC)/capillary electrophoresis.