This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Immunology, Subject name: Microbiology
DNA is detected by - 
Select and explain. 
A. Southern blot, B. Nohern blot, C. Western blot, D. Eastern blot
A: Southern blot
Answer: A: Southern blot 
Explanation: DNA fragments obtained by restriction enzyme digestion and separation on the gel can be transferred from the gel by blotting to nitrocellulose that binds the DNA. The DNA bound to the membrane is denatured and treated with radioactive single-stranded DNA probes. These will hyhybridizeith homologous DNA to form radioactive double stranded segments, which can be detected on X X-rail. This highly sensitive technique for identifying DNA fragments by DNA-DNA hybridization is called Southern blotting. It Reference: Anathanarayan & paniker's 9th edition, pg no:65 <\p>