This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Miscellaneous (Bio-Chemistry), Subject name: Biochemistry
Hydroxylation of Proline requires the following except: 
Select and explain. 
A. Fe2+, B. O2, C. Ascorbic Acid, D. Succinate
D: Succinate
Answer: D: Succinate 
Explanation: Ans. D. SuccinateVitamin C plays an important role as a co enzyme in the hydroxylation of Proline. The reaction is dependent on proline and requires vitamin C, alpha ketoglutarate, molecular O2 and Fe2+