This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of carbohydrate, Subject name: Biochemistry
Which of the following is required to bring about gluconeogenesis from pyruvate? 
Select and explain. 
A. Pyruvate dehydrogenase, B. Biotin, C. Alpha ketoglutarate dehydrogenase, D. Fructose-6 phosphate
B: Biotin
Answer: B: Biotin 
Explanation: Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalysed by a mitochondrial enzyme, pyruvate carboxylase (Fig. 9.24). It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, 7th edition, page no: 118