This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Bacteria, Subject name: Microbiology
Isolation of Chlamydia from tissue specimen can be done by: 
Select and explain. 
A. ELISA (Enzyme Linked Immune Assay), B. Yolk sac inoculation, C. Direct ImmunofluotescenceAntibody test (DFA), D. Polymerase Chain Reaction (PCR)
B: Yolk sac inoculation
Answer: B: Yolk sac inoculation 
Explanation: Ans. is 'b' i.e., Yolk sac inoculationRef: Harrison, 19th/e, p. 1165 and Ananthanarayan, 9th/e, p. 415Isolation of chlamydia can be done by using yolk sac or cell lines. NAAT detect DNA of bacteria so it is an identification test so bacteria cannot isolate by using this method.