This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Metabolism of carbohydrate, Subject name: Biochemistry
Which helps in the production of more glucose? 
Select and explain. 
A. Pyruvate kinase, B. Pyruvate carboxylase, C. PDH, D. Pyruvate decarboxylase
B: Pyruvate carboxylase
Answer: B: Pyruvate carboxylase 
Explanation: Reversal of the reaction catalyzed by pyruvate kinase in glycolysis involves two endothermic reactions. Mitochondrial pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate, Key Gluconeogenic Enzymes 1. Pyruvate carboxylase.Pyruvate Carboxylase Reaction Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalysed by a mitochondrial enzyme, pyruvate carboxylase (Fig. 9.24). It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, 7th edition, Chapter 9