This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Which of the following is not true regarding Nested PCR 
Select and explain. 
A. Two sets of Primers are used, B. Specificity of DNA amplification is increased, C. When DNA to be amplified is present in low concentration compared to total DNA this technique is most suitable, D. Sensitivity of DNA amplification is decreased
D: Sensitivity of DNA amplification is decreased
Answer: D: Sensitivity of DNA amplification is decreased 
Explanation: In Nested PCR, both sensitivity and specificity is increased.