This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pathology
The sensitive stain used to stain copper in Wilson's disease is: 
Select and explain. 
A. Timms sulfide, B. Rhodamine, C. Rubeanic acid, D. Trichrome stain
A: Timms sulfide
Answer: A: Timms sulfide 
Explanation: The copper is stained with rhodamine and rubeanic acid stains. More sensitive stain used is TIMMS SULFIDE. It is more effective in detecting cytoplasmic copper binding proteins. Trichrome stainning is used to show fibrous tissue. ALSO KNOW: The rhodanine stain is used to demonstrate excess copper in hepatocytes in Wilson's disease and also to demonstrate copper deposition in chronic biliary diseases. Ref: Schiffs disease of liver, Edition-11, Page-812