This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Mycology, Subject name: Microbiology
Coccidioides immitis is identified in tissues on the basis of which of the following 
Select and explain. 
A. Budding yeast cells with pseudohyphae, B. Yeast like forms with very large capsules, C. Chlamydospore, D. Endosporulating spherules
D: Endosporulating spherules
Answer: D: Endosporulating spherules 
Explanation: Laboratory diagnosis of coccidioidomycosis: 1. Demonstration of spherules containing endospores in: (a) sputum or smears from the lesion stained by calcofluor white and (b) in biopsy material stained by hematoxylin and eosin, silver, or PerIodic acid-Schiff stains. H & E staining