This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Genetics And Genetic Disorders, Subject name: Pediatrics
Submicroscopic deletions of any size can be detected by: 
Select and explain. 
A. Multiplex ligation-Dependent probe amplification (MLPA), B. Southern blotting, C. Cytogenomic array technology, D. Chromosome painting
A: Multiplex ligation-Dependent probe amplification (MLPA)
Answer: A: Multiplex ligation-Dependent probe amplification (MLPA) 
Explanation: a. Multiplex ligation-Dependent probe amplification(Ref: Nelson's 20/e p 605, Ghai 8/e p 6045)Multiplex Ligation-Dependent Probe Amplification (MLPA): It is a technique which mixes DNA hybridization, ligation, & PCR amplification to detect deletions & duplications of any size, including anomalies that are too large to be detected by PCR and too small to be identified by FISH.