This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Blood, Subject name: Medicine
Diagnostic test for paroxysmal nocturnal hemoglobinuria is: 
Select and explain. 
A. Sucrose lysis test, B. Ham test, C. Elevated LDH levels, D. Analysis of GPI-linked proteins
D: Analysis of GPI-linked proteins
Answer: D: Analysis of GPI-linked proteins 
Explanation: (Analysis of GPI-linked proteins) (616-H 16th) (437-CMDT-2009) (661-HI7lh)PAROXYSMAL NOCTURNAL HEMOGLOBINURIA -Diagnosis - Macro-ovalocytes, urine hemosiderin, T LDH, Iron deficiency anemia* TLC and platelet count decreased* Decreased leukocyte alkaline phosphatase* Bone marrow-generated hypoplasia or erythroid hyperplasia* Flow cytometric assays may confirm the diagnoses by demonstrating the absence of CD59 (503-CMDT-07)* Ham test (acidified serum lysis test) and sucrose lysis test (Reduction in ionic strength) are not reliable (616-H)* Analysis of GPI-linked protein (e.g. CD 59, DAF) on RBC and granulocytes by flow cytometry, (616-H)