This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Which organism cannot be cultured in cell free media -a) Klebsiella rhinoscleromatisb) Klebsiella ozaenaec) Treponema pallidumd) Pnemocystis jirovecie) Rhinosporidium seeberi 
Select and explain. 
A. abd, B. cde, C. bde, D. ade
B: cde
Answer: B: cde 
Explanation: Treponema pallidum is an abligate intracellular organism. Thus it cannot be cultivated in cell free medium.
Pneumocystis Jiroveci and Rhinosporidium seeberi can not be cultivated.