This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Thalassemia, Subject name: Medicine
In alpha-thalassemia trait, electrophoresis shows: 
Select and explain. 
A. Increased HbF & normal HbA2, B. Normal HbF & normal HbA2, C. Normal HbF & decreased HbA2, D. Decreased HbF & normal HbA2
B: Normal HbF & normal HbA2
Answer: B: Normal HbF & normal HbA2 
Explanation: Electrophoresis is a screening test for hemoglobinopathies Normal individual Sickle cell anaemia a thalassemia b thalassemia HbA 95% Absent Less HbF 2% Present Normal Increased HbA2 3% Present Normal Increased Note: In sickle cell anemia absence of HbA means the disease severity is very high. But in sickle cell trait HBA is present. Note: Screening test for both a & b thalassemia is NESTROF IOC for both a & b thalassemia is HPLC