This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Unknown
Volume of distribution is NOT affected by all EXCEPT 
Select and explain. 
A. Binding to plasma protein, B. Drug clearance, C. Lipid insolubility, D. Absence of blood brain barrier
A: Binding to plasma protein
Answer: A: Binding to plasma protein 
Explanation: (Binding to plasma protein) (31-33-KDT 6th)* The extent of distribution of drug depends on* Lipid solubility* Ionization at physiological pH (dependent on pKa)* The extent of binding to plasma and tissue proteins* Differences in regional bloodflow