This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: General pathology, Subject name: Pathology
Which of the following procedures as routine technique for karyotyping using light microscopy 
Select and explain. 
A. C-banding, B. G-banding, C. Q-banding, D. Brd V-staining
B: G-banding
Answer: B: G-banding 
Explanation: A karyotype is a photographic representation of a stained metaphase spread in which the chromosomes are arranged in order of decreasing length. With the widely used Giemsa stain (G banding) technique, each chromosome set can be seen to possess a distinctive pattern of alternating light and dark bands of variable width. The use of banding techniques allows identification of each chromosome, and can detect and localize structural abnormalities (Robbins Basic Pathology, 9 th edition. page : 234)