This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Carbohydrates, Subject name: Biochemistry
In conversion of lactic acid to glucose, three reactions of glycolytic pathway are circumvented, which of the following enzymes do not participate? 
Select and explain. 
A. Pyruvate carboxylase, B. Phosphoenol pyruvate carboxy kinase, C. Pyruvate kinase, D. Glucose-6-phosphatase
C: Pyruvate kinase
Answer: C: Pyruvate kinase 
Explanation: Ans. C. Pyruvate kinasePyruvate Kinase is the enzyme used in glycolysis. In the conversion of Lactic Acid to glucose the three steps of glycolysis are made reversible. To convert pyruvate to PEP-----Pyruvate carboxy kinase and PEP carboxy kinase are utilized.