This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Which of the following is used to study protein-protein interaction ? 
Select and explain. 
A. Western blot, B. Affinity electrophoresis, C. Thin-layer chromatography, D. None
B: Affinity electrophoresis
Answer: B: Affinity electrophoresis 
Explanation: Ans. is 'b' i.e., Affinity electrophoresis The most conventional and widely used high-through put methods for protein-protein interactions (PPI) are yeast two-hybrid screening and affinity purification coupled to mass spectroscopy. Other methods used to study PPI are :- LI Affinity electrophoresis Co-immunoprecipitation Protein microarrays Analytical ultracentrifugation Light scattering Fluorescence spectroscopy SPINE (strep-protein interaction experiment) LUMIER (luminescence-based mammalian intractome mapping) Calorimetry QUICK (quantitative immunoprecipitation combined with knock-down) BiFC (Biomolecular fluorescence complementation) PLA (Proximity ligation assay) Resonance energy transfer system Mammalian protein-protein interaction trap Surface plasma resonance Protein-fragment complementation assay Tandom affinity purification