This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Medical Jurisprudence, Subject name: Forensic Medicine
Spermin is detected in semen by which test? 
Select and explain. 
A. Barberio test, B. Florence test, C. ELISA, D. Agglutination inhibition
A: Barberio test
Answer: A: Barberio test 
Explanation: Seminal Chemical Examination:The rapid tests are Florence test (based on the exhibition of dark brown rhombic crystals of choline iodide, resembling haemin but are larger, arranged in clusters, rosettes or crosses, etc.) and Barberio test (showing yellow needle-shaped crystals of spermine picrate). Both these tests are simply suggestive of the presence of some vegetable or animal substance. A negative test, however, reveals that the stain is not of seminal origin.Ref: Krishan Vij textbook of forensic medicine and toxicology; 5th edition; Page no: 324