This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Which is not a step of PCR ? 
Select and explain. 
A. Annealing, B. Extension, C. Transformation, D. Denaturation
C: Transformation
Answer: C: Transformation 
Explanation: Ans. is 'c' i.e., Transformation Steps in PCRIsolation of target DNA sequence- Primer construction - Denaturation of DNA- Annealing of primers to single stranded DNA- Chain extension.