This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Medicine
Gene expression can be detected by all except: 
Select and explain. 
A. #NAME?, B. cDNA microarray, C. Nohern blot, D. Southern blot and Immunohistochemistry
D: Southern blot and Immunohistochemistry
Answer: D: Southern blot and Immunohistochemistry 
Explanation: C i.e. Southern Blot and ImmunohistochemistryImmunohisto chemistry detects antigens (eg proteins) in abnormal (eg cancerous) or normal cells of a tissue by antibodies binding specifically to that antigen (so immune means antigen-antibody binding and histo means in tissue). It is used to find distribution & localization of biological marker in various tissues.