This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: Techniques in molecular biology, Subject name: Biochemistry
Which of the following method is used for the analysis of the C-terminal end of a polypeptide? 
Select and explain. 
A. Sanger's method, B. Edman's degradation method, C. Akabori method, D. None of the above
C: Akabori method
Answer: C: Akabori method 
Explanation: Akabori method is used for the analysis of C-terminal end of a polypeptide N-terminal analysis Carboxy-terminal analysis For post-translational modifications Sanger's reagent- fluoro-2,4 dinitrobenzene (FDNB) Carboxypeptidase assay Mass spectrometry Edman's reagent- Phenylisothiocyanate (PITC) Akabori reaction