This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Which statements are true about E. coli chromosomal DNA in relation with eukaryotic chromosomal DNA 
Select and explain. 
A. Circular, B. Negatively supercoiled, C. Nucleoid present, D. All of these
D: All of these
Answer: D: All of these 
Explanation: E.coli (Bacteria) has single, closed, circular, ds DNA which is negatively supercoiled, present in nucleoid. There is no nucleolus in bacteria.