This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Which of the following is used for isolation fo chlamydia - 
Select and explain. 
A. Microimmunofluoroscence, B. Yolk sac ioculation, C. ELISA, D. Immunofluroscence
B: Yolk sac ioculation
Answer: B: Yolk sac ioculation 
Explanation: Ans. is 'b' i.e., Yolk sac Isolation of chlammydia . Chlamydiae are obligate intracellular parsite, therefore cannot be cultured on nonliving aificial media (cell free media). . Isolation of chalamydia can be done by inoculation into :? Embryonated eggs paicularly in yolk. Experimental animals Tissue culture (cell culture, e.g., McCoy cells, HeLa cells). . Amongst these, cell culture is the preferred method of isolation.