This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Pathology
True about apoptosis- 
Select and explain. 
A. Migration of Leukocytes, B. End products are phagocytosed by macrophage, C. Intranuclear fragmentation of DNA, D. All
D: All
Answer: D: All 
Explanation: Ans. is 'b' i.e., End products are phagocytosed by macrophage; 'c' i.e., Intranuclear fragmentation of DNAo As there is no inflammation in apoptosis --> there is no leukocyte migration.Apoptotic cells or apoptotic bodies (end products) are phagocytosed by macrophages.o Endonuclease causes double stranded breaks of nuclear DNA. The nucleus itself may break up producing two or more fragments.o Activation of caspases is central to the pathogenesis of apoptosis.o Following are the method to identify the apoptopic cells : Staining of chromatin condension by haematoxylin, Feulgen and acridine orange. Flow cytometry to visualise rapid cell shrinkage. DNA changes in situ technique or by electrophoresis. Annexin Vas a marker of apoptotic cell.