Description of Croceitalea gen. nov. in the family Flavobacteriaceae with two species, Croceitalea eckloniae sp. nov. and Croceitalea dokdonensis sp. nov., isolated from the rhizosphere of the marine alga Ecklonia kurome
Hee-Soon Lee1, Kae Kyoung Kwon1, Sung-Hyun Yang1, Seung Seob Bae1, Chan Hong Park2, Sang-Jin Kim1, Jung-Hyun Lee1
Affiliations: 1 1Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea 2 2Dokdo Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea
CorrespondenceJung-Hyun Lee
[email protected]
First Published: 01 November 2008 https://doi.org/10.1099/ijs.0.65697-0
Two novel bacterial strains, designated DOKDO 025T and DOKDO 023T, were isolated on Dokdo Island, Korea, from the rhizosphere of the brown alga Ecklonia kurome. The strains were subjected to a polyphasic taxonomy study and were found to be Gram-negative, aerobic, rod-shaped, non-motile and orange-coloured. The isolates shared 96.3 % 16S rRNA gene sequence similarity. They showed 93.8–95.6 % 16S rRNA gene sequence similarity with respect to members of the genus Muricauda in the family Flavobacteriaceae, but formed a distinct phyletic line. Moreover, the cellular appendages reported for all Muricauda species were absent from strains DOKDO 025T and DOKDO 023T. The predominant cellular fatty acids of strain DOKDO 025T were iso-C15 : 0, iso-C15 : 1 and one with an equivalent chain-length of 13.565 and those of strain DOKDO 023T were iso-C15 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH. The DNA G+C content of strains DOKDO 025T and DOKDO 023T were 59.5 and 66.5 mol%, respectively, higher than any values found in recognized members of the family Flavobacteriaceae. The major respiratory quinone was MK-6. On the basis of evidence from the polyphasic study, strains DOKDO 025T and DOKDO 023T represent two novel species in a new genus, Croceitalea gen. nov., for which the names Croceitalea eckloniae sp. nov. (the type species) and Croceitalea dokdonensis sp. nov. are proposed. The type strain of Croceitalea eckloniae sp. nov. is DOKDO 025T (=KCCM 42309T =JCM 13827T) and that of Croceitalea dokdonensis sp. nov. is DOKDO 023T (=KCCM 42308T =JCM 13826T).
Keyword(s): ECL, equivalent chain-length
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Int J Syst Evol Microbiol 58, 2505 (2008); https://doi.org/10.1099/ijs.0.65697-0
Supplementary Fig. S1. Scanning electron micrographs of cells of Croceitalea eckloniae DOKDO 025 T(a) and Croceitalea dokdonensis DOKDO 023 T(b) grown in MB for 3 days at 25 °C.
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10.1099/ijs.0.65697-0