The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture
T. Takano, M. Takigawa, E. Shirai, K. Nakagawa, M. Sakuda, F. Suzuki
Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1—34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10-7 mol/L) were greatest in MCCchondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.
Journal of dental research
https://doi.org/10.1177/00220345870660011801
Dentistry(all)
10.1177/00220345870660011801
Dive into the research topics of 'The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture'. Together they form a unique fingerprint.
Nasal Cartilages Medicine & Life Sciences 100%
Ornithine Decarboxylase Medicine & Life Sciences 84%
Glycosaminoglycans Medicine & Life Sciences 69%
Chondrocytes Medicine & Life Sciences 66%
Parathyroid Hormone Medicine & Life Sciences 65%
Cyclic AMP Medicine & Life Sciences 64%
Cartilage Medicine & Life Sciences 57%
bovine parathyroid hormone Medicine & Life Sciences 22%
Takano, T., Takigawa, M., Shirai, E., Nakagawa, K., Sakuda, M., & Suzuki, F. (1987). The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture. Journal of dental research, 66(1), 84-87. https://doi.org/10.1177/00220345870660011801
The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture. / Takano, T.; Takigawa, M.; Shirai, E.; Nakagawa, K.; Sakuda, M.; Suzuki, F.
In: Journal of dental research, Vol. 66, No. 1, 01.1987, p. 84-87.
Takano, T, Takigawa, M, Shirai, E, Nakagawa, K, Sakuda, M & Suzuki, F 1987, 'The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture', Journal of dental research, vol. 66, no. 1, pp. 84-87. https://doi.org/10.1177/00220345870660011801
Takano T, Takigawa M, Shirai E, Nakagawa K, Sakuda M, Suzuki F. The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture. Journal of dental research. 1987 Jan;66(1):84-87. https://doi.org/10.1177/00220345870660011801
Takano, T. ; Takigawa, M. ; Shirai, E. ; Nakagawa, K. ; Sakuda, M. ; Suzuki, F. / The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture. In: Journal of dental research. 1987 ; Vol. 66, No. 1. pp. 84-87.
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title = "The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture",
abstract = "Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1—34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10-7 mol/L) were greatest in MCCchondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.",
author = "T. Takano and M. Takigawa and E. Shirai and K. Nakagawa and M. Sakuda and F. Suzuki",
doi = "10.1177/00220345870660011801",
journal = "Journal of Dental Research",
publisher = "SAGE Publications Inc.",
T1 - The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture
AU - Takano, T.
AU - Takigawa, M.
AU - Shirai, E.
AU - Nakagawa, K.
AU - Sakuda, M.
AU - Suzuki, F.
N2 - Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1—34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10-7 mol/L) were greatest in MCCchondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.
AB - Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1—34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10-7 mol/L) were greatest in MCCchondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.
U2 - 10.1177/00220345870660011801
DO - 10.1177/00220345870660011801
JO - Journal of Dental Research
JF - Journal of Dental Research