Rapid purification of intact tonofilaments from newborn rats: Comparison with glial filaments and neurofilaments
Shu Hui Yen, Ronald K.H. Liem, Lin Tai Jenq, Michael L. Shelanski
A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58000 and 66000 D. An antiserum prepared against the 58000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.
https://doi.org/10.1016/0014-4827(80)90498-X
10.1016/0014-4827(80)90498-X
Dive into the research topics of 'Rapid purification of intact tonofilaments from newborn rats: Comparison with glial filaments and neurofilaments'. Together they form a unique fingerprint.
Intermediate Filaments Medicine & Life Sciences 100%
Protein Subunits Medicine & Life Sciences 34%
Tubulin Medicine & Life Sciences 32%
Immune Sera Medicine & Life Sciences 27%
Indirect Fluorescent Antibody Technique Medicine & Life Sciences 16%
Epidermis Medicine & Life Sciences 15%
Polyacrylamide Gel Electrophoresis Medicine & Life Sciences 14%
Yen, S. H., Liem, R. K. H., Jenq, L. T., & Shelanski, M. L. (1980). Rapid purification of intact tonofilaments from newborn rats: Comparison with glial filaments and neurofilaments. Experimental Cell Research, 129(2), 313-320. https://doi.org/10.1016/0014-4827(80)90498-X
Rapid purification of intact tonofilaments from newborn rats : Comparison with glial filaments and neurofilaments. / Yen, Shu Hui; Liem, Ronald K.H.; Jenq, Lin Tai; Shelanski, Michael L.
In: Experimental Cell Research, Vol. 129, No. 2, 1980, p. 313-320.
Yen, SH, Liem, RKH, Jenq, LT & Shelanski, ML 1980, 'Rapid purification of intact tonofilaments from newborn rats: Comparison with glial filaments and neurofilaments', Experimental Cell Research, vol. 129, no. 2, pp. 313-320. https://doi.org/10.1016/0014-4827(80)90498-X
Yen SH, Liem RKH, Jenq LT, Shelanski ML. Rapid purification of intact tonofilaments from newborn rats: Comparison with glial filaments and neurofilaments. Experimental Cell Research. 1980;129(2):313-320. https://doi.org/10.1016/0014-4827(80)90498-X
Yen, Shu Hui ; Liem, Ronald K.H. ; Jenq, Lin Tai ; Shelanski, Michael L. / Rapid purification of intact tonofilaments from newborn rats : Comparison with glial filaments and neurofilaments. In: Experimental Cell Research. 1980 ; Vol. 129, No. 2. pp. 313-320.
@article{c833c808445b42cb847b09b739979092,
title = "Rapid purification of intact tonofilaments from newborn rats: Comparison with glial filaments and neurofilaments",
abstract = "A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58000 and 66000 D. An antiserum prepared against the 58000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.",
author = "Yen, {Shu Hui} and Liem, {Ronald K.H.} and Jenq, {Lin Tai} and Shelanski, {Michael L.}",
note = "Funding Information: This work was supported by grants from the National Institute of Neurological, Communicative Disorders and Stroke (NS-I 15041 and the McKnight Foundation. S.-H. Y. and R. K. H. L. were. respectively. Post-doctoral Trainee and Post-doctoral Fellow of the National Institute of Neurological. Communicative Disorders and Stroke.",
doi = "10.1016/0014-4827(80)90498-X",
T1 - Rapid purification of intact tonofilaments from newborn rats
T2 - Comparison with glial filaments and neurofilaments
AU - Yen, Shu Hui
AU - Liem, Ronald K.H.
AU - Jenq, Lin Tai
AU - Shelanski, Michael L.
N1 - Funding Information: This work was supported by grants from the National Institute of Neurological, Communicative Disorders and Stroke (NS-I 15041 and the McKnight Foundation. S.-H. Y. and R. K. H. L. were. respectively. Post-doctoral Trainee and Post-doctoral Fellow of the National Institute of Neurological. Communicative Disorders and Stroke.
N2 - A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58000 and 66000 D. An antiserum prepared against the 58000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.
AB - A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58000 and 66000 D. An antiserum prepared against the 58000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.
U2 - 10.1016/0014-4827(80)90498-X
DO - 10.1016/0014-4827(80)90498-X