openAccess (113)
Structure-properties relationships of natural and synthetic molecules and their metal complexes
Structure-properties relationships of natural and synthetic molecules and their metal complexes (en)
Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (sr)
Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (sr_RS)
Tešić, Živoslav Lj. Milojković-Opsenica, Dušanka Gašić, Uroš M.
Natić, Maja Trifković, Jelena Dabić-Zagorac, Dragana
Ristivojević, Petar Andrić, Filip Fotirić-Akšić, Milica M.
Sredojević, Milica Tosti, Tomislav Mutić, Jelena
Pešić, Mirjana B. Kostić, Aleksandar Ž. Dramićanin, Aleksandra M.
Šegan, Sandra B. Baošić, Rada Brčeski, Ilija
Milinčić, Danijel D. Mišić, Danijela Radoičić, Aleksandra
Veljović, Sonja Stanković, Dalibor Lušić, Dražen
Smailagić, Anita Stevanović, Nikola R. Vukojević, Vesna
Dimkić, Ivica Vovk, Irena Đurđić, Slađana Z.
Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds
Ristivojević, Petar; Jovanović, Vesna B.; Milojković-Opsenica, Dušanka; Park, Jihae; Rollinger, Judith M.; Ćirković-Veličković, Tanja
AU  - Jovanović, Vesna B.
AU  - Park, Jihae
AU  - Rollinger, Judith M.
AB  - Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.
T1  - Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds
author = "Ristivojević, Petar and Jovanović, Vesna B. and Milojković-Opsenica, Dušanka and Park, Jihae and Rollinger, Judith M. and Ćirković-Veličković, Tanja",
abstract = "Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.",
title = "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds",
Ristivojević, P., Jovanović, V. B., Milojković-Opsenica, D., Park, J., Rollinger, J. M.,& Ćirković-Veličković, T.. (2021). Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry
Ristivojević P, Jovanović VB, Milojković-Opsenica D, Park J, Rollinger JM, Ćirković-Veličković T. Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry. 2021;334:127562.
Ristivojević, Petar, Jovanović, Vesna B., Milojković-Opsenica, Dušanka, Park, Jihae, Rollinger, Judith M., Ćirković-Veličković, Tanja, "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds" in Food Chemistry, 334 (2021):127562,
Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.
T1  - Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.
title = "Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.",
Ristivojević, P., Jovanović, V. B., Milojković-Opsenica, D., Park, J., Rollinger, J. M.,& Ćirković-Veličković, T.. (2021). Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.. in Food Chemistry
Ristivojević P, Jovanović VB, Milojković-Opsenica D, Park J, Rollinger JM, Ćirković-Veličković T. Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.. in Food Chemistry. 2021;.
Ristivojević, Petar, Jovanović, Vesna B., Milojković-Opsenica, Dušanka, Park, Jihae, Rollinger, Judith M., Ćirković-Veličković, Tanja, "Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562." in Food Chemistry (2021),
Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition
Čolić, Slavica D.; Bakić, Ivana V.; Dabić-Zagorac, Dragana; Natić, Maja; Smailagić, Anita; Pergal, Marija V.; Pešić, Mirjana B.; Milinčić, Danijel D.; Rabrenović, Biljana B.; Fotirić-Akšić, Milica M.
AU  - Čolić, Slavica D.
AU  - Bakić, Ivana V.
AU  - Natić, Maja
AU  - Smailagić, Anita
AU  - Pergal, Marija V.
AU  - Rabrenović, Biljana B.
AB  - The study was designed to investigate chemical composition and kernel quality of almond cultivars 'Troito', 'Marcona' and 'Texas' grafted on Myrobalan plum seedlings and 'GF 677' rootstock under drought stress. Oil content varied from 53.34% ('Marcona') to 58.11% ('Texas'), and protein content was in a range from 17.36% ('Texas') to 23.02% ('Troito'). 'GF 677' contributed to a higher content of oleic and lower content of linoleic acid during favourable conditions but had the opposite trend during drought. Regarding flavanols, the most abundant in all three cultivars was epigalocatechin, followed by catechin. From the results it is concluded that scions on 'GF 677' were better adapted to water stress conditions in comparison with Myrobalan plum seedlings as they had significantly higher kernel weight, kernel ratio, and yield per tree. Also, kernel quality attributes, contents of proteins, oleic acid, and oleic acid/linoleic acid ratio were higher, while the content of amandin decreased. Furthermore, an increase of flavanols (especially epigallocatechin gallate) on 'GF 677' could have led to the enhancement of drought tolerance. Besides the fact that water deficit decreased the majority of chemical components in almond kernels, the grafting combination 'Texas'/'GF 677' can be grown in drought conditions without significant loss of kernel quality.
T2  - Scientia Horticulturae
T1  - Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition
DO  - 10.1016/j.scienta.2020.109705
author = "Čolić, Slavica D. and Bakić, Ivana V. and Dabić-Zagorac, Dragana and Natić, Maja and Smailagić, Anita and Pergal, Marija V. and Pešić, Mirjana B. and Milinčić, Danijel D. and Rabrenović, Biljana B. and Fotirić-Akšić, Milica M.",
abstract = "The study was designed to investigate chemical composition and kernel quality of almond cultivars 'Troito', 'Marcona' and 'Texas' grafted on Myrobalan plum seedlings and 'GF 677' rootstock under drought stress. Oil content varied from 53.34% ('Marcona') to 58.11% ('Texas'), and protein content was in a range from 17.36% ('Texas') to 23.02% ('Troito'). 'GF 677' contributed to a higher content of oleic and lower content of linoleic acid during favourable conditions but had the opposite trend during drought. Regarding flavanols, the most abundant in all three cultivars was epigalocatechin, followed by catechin. From the results it is concluded that scions on 'GF 677' were better adapted to water stress conditions in comparison with Myrobalan plum seedlings as they had significantly higher kernel weight, kernel ratio, and yield per tree. Also, kernel quality attributes, contents of proteins, oleic acid, and oleic acid/linoleic acid ratio were higher, while the content of amandin decreased. Furthermore, an increase of flavanols (especially epigallocatechin gallate) on 'GF 677' could have led to the enhancement of drought tolerance. Besides the fact that water deficit decreased the majority of chemical components in almond kernels, the grafting combination 'Texas'/'GF 677' can be grown in drought conditions without significant loss of kernel quality.",
journal = "Scientia Horticulturae",
title = "Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition",
doi = "10.1016/j.scienta.2020.109705"
Čolić, S. D., Bakić, I. V., Dabić-Zagorac, D., Natić, M., Smailagić, A., Pergal, M. V., Pešić, M. B., Milinčić, D. D., Rabrenović, B. B.,& Fotirić-Akšić, M. M.. (2021). Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. in Scientia Horticulturae
https://doi.org/10.1016/j.scienta.2020.109705
Čolić SD, Bakić IV, Dabić-Zagorac D, Natić M, Smailagić A, Pergal MV, Pešić MB, Milinčić DD, Rabrenović BB, Fotirić-Akšić MM. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. in Scientia Horticulturae. 2021;275:109705.
doi:10.1016/j.scienta.2020.109705 .
Čolić, Slavica D., Bakić, Ivana V., Dabić-Zagorac, Dragana, Natić, Maja, Smailagić, Anita, Pergal, Marija V., Pešić, Mirjana B., Milinčić, Danijel D., Rabrenović, Biljana B., Fotirić-Akšić, Milica M., "Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition" in Scientia Horticulturae, 275 (2021):109705,
https://doi.org/10.1016/j.scienta.2020.109705 . .
Supplementary data for the article: Čolić, S. D.; Bakić, I. V.; Dabić Zagorac, D. Č.; Natić, M. M.; Smailagić, A. T.; Pergal, M. V.; Pešić, M. B.; Milinčić, D. D.; Rabrenović, B. B.; Fotirić Akšić, M. M. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. Scientia Horticulturae 2021, 275, 109705. https://doi.org/10.1016/j.scienta.2020.109705
T1  - Supplementary data for the article: Čolić, S. D.; Bakić, I. V.; Dabić Zagorac, D. Č.; Natić, M. M.; Smailagić, A. T.; Pergal, M. V.; Pešić, M. B.; Milinčić, D. D.; Rabrenović, B. B.; Fotirić Akšić, M. M. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. Scientia Horticulturae 2021, 275, 109705. https://doi.org/10.1016/j.scienta.2020.109705
title = "Supplementary data for the article: Čolić, S. D.; Bakić, I. V.; Dabić Zagorac, D. Č.; Natić, M. M.; Smailagić, A. T.; Pergal, M. V.; Pešić, M. B.; Milinčić, D. D.; Rabrenović, B. B.; Fotirić Akšić, M. M. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. Scientia Horticulturae 2021, 275, 109705. https://doi.org/10.1016/j.scienta.2020.109705",
Čolić, S. D., Bakić, I. V., Dabić-Zagorac, D., Natić, M., Smailagić, A., Pergal, M. V., Pešić, M. B., Milinčić, D. D., Rabrenović, B. B.,& Fotirić-Akšić, M. M.. (2021). Supplementary data for the article: Čolić, S. D.; Bakić, I. V.; Dabić Zagorac, D. Č.; Natić, M. M.; Smailagić, A. T.; Pergal, M. V.; Pešić, M. B.; Milinčić, D. D.; Rabrenović, B. B.; Fotirić Akšić, M. M. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. Scientia Horticulturae 2021, 275, 109705. https://doi.org/10.1016/j.scienta.2020.109705. in Scientia Horticulturae
Čolić SD, Bakić IV, Dabić-Zagorac D, Natić M, Smailagić A, Pergal MV, Pešić MB, Milinčić DD, Rabrenović BB, Fotirić-Akšić MM. Supplementary data for the article: Čolić, S. D.; Bakić, I. V.; Dabić Zagorac, D. Č.; Natić, M. M.; Smailagić, A. T.; Pergal, M. V.; Pešić, M. B.; Milinčić, D. D.; Rabrenović, B. B.; Fotirić Akšić, M. M. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. Scientia Horticulturae 2021, 275, 109705. https://doi.org/10.1016/j.scienta.2020.109705. in Scientia Horticulturae. 2021;.
Čolić, Slavica D., Bakić, Ivana V., Dabić-Zagorac, Dragana, Natić, Maja, Smailagić, Anita, Pergal, Marija V., Pešić, Mirjana B., Milinčić, Danijel D., Rabrenović, Biljana B., Fotirić-Akšić, Milica M., "Supplementary data for the article: Čolić, S. D.; Bakić, I. V.; Dabić Zagorac, D. Č.; Natić, M. M.; Smailagić, A. T.; Pergal, M. V.; Pešić, M. B.; Milinčić, D. D.; Rabrenović, B. B.; Fotirić Akšić, M. M. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. Scientia Horticulturae 2021, 275, 109705. https://doi.org/10.1016/j.scienta.2020.109705" in Scientia Horticulturae (2021),
Nondestructive optical method for plant overall health evaluation
Kasalica, Bećko V.; Miletić, Katarina M.; Sabovljević, Aneta D.; Vujičić, Milorad M.; Jeremić, Dejan A.; Belča, Ivan D.; Petković-Benazzouz, Marija M.
AU  - Kasalica, Bećko V.
AU  - Miletić, Katarina M.
AU  - Sabovljević, Aneta D.
AU  - Vujičić, Milorad M.
AU  - Jeremić, Dejan A.
AU  - Belča, Ivan D.
AU  - Petković-Benazzouz, Marija M.
AB  - In vivo measurements of the optical properties of plant leaves, by spectrophotometric methods, could potentially provide very useful information for the assessment of the plant's health. This paper describes a novel experimental set-up that enables continuous measurements of the optical reflection and transmission coefficients of broad-leaved plants. The optical spectral characteristics of leaves, in the broad wavelength range, are often a discussed topic in the scientific literature. Nevertheless, to the best of our knowledge, the real-time spectral analyses of the leaf haven't been reported so far. Monitoring the evolution of the plant activity in real-time has resulted in the graph of the spectral Circadian rhythm as a function of time. The set-up was tested on Phaseolus vulgaris and the results of the measurements are presented and discussed.
T2  - Acta Agriculturae Scandinavica, Section B — Soil & Plant Science
T1  - Nondestructive optical method for plant overall health evaluation
EP  - 7
author = "Kasalica, Bećko V. and Miletić, Katarina M. and Sabovljević, Aneta D. and Vujičić, Milorad M. and Jeremić, Dejan A. and Belča, Ivan D. and Petković-Benazzouz, Marija M.",
abstract = "In vivo measurements of the optical properties of plant leaves, by spectrophotometric methods, could potentially provide very useful information for the assessment of the plant's health. This paper describes a novel experimental set-up that enables continuous measurements of the optical reflection and transmission coefficients of broad-leaved plants. The optical spectral characteristics of leaves, in the broad wavelength range, are often a discussed topic in the scientific literature. Nevertheless, to the best of our knowledge, the real-time spectral analyses of the leaf haven't been reported so far. Monitoring the evolution of the plant activity in real-time has resulted in the graph of the spectral Circadian rhythm as a function of time. The set-up was tested on Phaseolus vulgaris and the results of the measurements are presented and discussed.",
journal = "Acta Agriculturae Scandinavica, Section B — Soil & Plant Science",
title = "Nondestructive optical method for plant overall health evaluation",
pages = "1-7",
doi = "10.1080/09064710.2021.1928740, 10.1080/09064710.2021.1928740"
Kasalica, B. V., Miletić, K. M., Sabovljević, A. D., Vujičić, M. M., Jeremić, D. A., Belča, I. D.,& Petković-Benazzouz, M. M.. (2021). Nondestructive optical method for plant overall health evaluation. in Acta Agriculturae Scandinavica, Section B — Soil & Plant Science
Taylor & Francis., 1-7.
Kasalica BV, Miletić KM, Sabovljević AD, Vujičić MM, Jeremić DA, Belča ID, Petković-Benazzouz MM. Nondestructive optical method for plant overall health evaluation. in Acta Agriculturae Scandinavica, Section B — Soil & Plant Science. 2021;:1-7.
Kasalica, Bećko V., Miletić, Katarina M., Sabovljević, Aneta D., Vujičić, Milorad M., Jeremić, Dejan A., Belča, Ivan D., Petković-Benazzouz, Marija M., "Nondestructive optical method for plant overall health evaluation" in Acta Agriculturae Scandinavica, Section B — Soil & Plant Science (2021):1-7,
Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
Stikić, Radmila I.; Milinčić, Danijel D.; Kostić, Aleksandar Ž.; Jovanović, Zorica; Gašić, Uroš M.; Tešić, Živoslav Lj.; Djordjević, Nataša Z.; Savić, Slađana K.; Czekus, Borisz G.; Pešić, Mirjana B.
(Wiley-Blackwell, 2020)
AU  - Stikić, Radmila I.
AU  - Jovanović, Zorica
AU  - Djordjević, Nataša Z.
AU  - Savić, Slađana K.
AU  - Czekus, Borisz G.
AB  - Background and objectives: Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings: Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions: The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty: The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.
PB  - Wiley-Blackwell
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
DO  - 10.1002/cche.10278
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica and Gašić, Uroš M. and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Slađana K. and Czekus, Borisz G. and Pešić, Mirjana B.",
abstract = "Background and objectives: Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings: Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions: The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty: The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
doi = "10.1002/cche.10278"
Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z., Gašić, U. M., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M. B.. (2020). Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry
Wiley-Blackwell., 97(3), 626-633.
https://doi.org/10.1002/cche.10278
Stikić RI, Milinčić DD, Kostić AŽ, Jovanović Z, Gašić UM, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić MB. Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):626-633.
doi:10.1002/cche.10278 .
Stikić, Radmila I., Milinčić, Danijel D., Kostić, Aleksandar Ž., Jovanović, Zorica, Gašić, Uroš M., Tešić, Živoslav Lj., Djordjević, Nataša Z., Savić, Slađana K., Czekus, Borisz G., Pešić, Mirjana B., "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):626-633,
https://doi.org/10.1002/cche.10278 . .
Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
Dabić-Zagorac, Dragana; Fotirić-Akšić, Milica M.; Glavnik, Vesna; Gašić, Uroš M.; Vovk, Irena; Tešić, Živoslav Lj.; Natić, Maja
AU  - Glavnik, Vesna
AB  - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
DO  - 10.1002/jssc.201901271
author = "Dabić-Zagorac, Dragana and Fotirić-Akšić, Milica M. and Glavnik, Vesna and Gašić, Uroš M. and Vovk, Irena and Tešić, Živoslav Lj. and Natić, Maja",
abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
doi = "10.1002/jssc.201901271"
Dabić-Zagorac, D., Fotirić-Akšić, M. M., Glavnik, V., Gašić, U. M., Vovk, I., Tešić, Ž. Lj.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science, 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271
Dabić-Zagorac D, Fotirić-Akšić MM, Glavnik V, Gašić UM, Vovk I, Tešić ŽL, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .
Dabić-Zagorac, Dragana, Fotirić-Akšić, Milica M., Glavnik, Vesna, Gašić, Uroš M., Vovk, Irena, Tešić, Živoslav Lj., Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .
Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts
Smailagić, Anita; Ristivojević, Petar; Dimkić, Ivica; Pavlović, Tamara; Dabić-Zagorac, Dragana; Veljović, Sonja; Fotirić-Akšić, Milica M.; Meland, Mekjell; Natić, Maja
AU  - Dimkić, Ivica
AU  - Pavlović, Tamara
AU  - Veljović, Sonja
AB  - The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak (Quercus petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.), mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), and wild cherry (Prunus avium L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed di erent chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or ca eic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: Quercus spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against Staphylococcus aureus for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.
T1  - Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts
DO  - 10.3390/foods9030319
author = "Smailagić, Anita and Ristivojević, Petar and Dimkić, Ivica and Pavlović, Tamara and Dabić-Zagorac, Dragana and Veljović, Sonja and Fotirić-Akšić, Milica M. and Meland, Mekjell and Natić, Maja",
abstract = "The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak (Quercus petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.), mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), and wild cherry (Prunus avium L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed di erent chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or ca eic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: Quercus spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against Staphylococcus aureus for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.",
title = "Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts",
doi = "10.3390/foods9030319"
Smailagić, A., Ristivojević, P., Dimkić, I., Pavlović, T., Dabić-Zagorac, D., Veljović, S., Fotirić-Akšić, M. M., Meland, M.,& Natić, M.. (2020). Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts. in Foods
MDPI., 9(3), 319.
Smailagić A, Ristivojević P, Dimkić I, Pavlović T, Dabić-Zagorac D, Veljović S, Fotirić-Akšić MM, Meland M, Natić M. Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts. in Foods. 2020;9(3):319.
doi:10.3390/foods9030319 .
Smailagić, Anita, Ristivojević, Petar, Dimkić, Ivica, Pavlović, Tamara, Dabić-Zagorac, Dragana, Veljović, Sonja, Fotirić-Akšić, Milica M., Meland, Mekjell, Natić, Maja, "Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts" in Foods, 9, no. 3 (2020):319,
https://doi.org/10.3390/foods9030319 . .
Gathered fruits as grave goods? Cornelian cherry remains from a Mesolithic grave at the site of Vlasac, Danube Gorges, south-east Europe
Filipović, Dragana; Fotirić-Akšić, Milica M.; Dabić-Zagorac, Dragana; Natić, Maja
AU  - Filipović, Dragana
AB  - Establishing the use and purpose of wild plant remains recovered from archaeological layers is in many cases far from straightforward. When discovered in a mortuary context, they are generally interpreted as remnants of ritual eating or drinking, offerings to the dead, or mnemonics, if not as accidental inclusions in the grave fills. In the Mesolithic cremation graves at the site of Vlasac in the Danube Gorges, south-east Europe, Cornelian cherry (Cornus mas L.) fruit stones were discovered; in one case, their number was relatively high. Whereas the fruit stones could simply be regarded as contamination in the burial fill, this study investigates an alternative possibility – that the cherries, or just fruit stones, represented an element of the Mesolithic funerary ritual at Vlasac. The archaeological context in which this plant material was discovered is taken as fundamental to the understanding of taphonomy of the botanical evidence and so it was considered in detail. Examination of the relevant morphological properties and the experimental charring of modern Cornelian cherry fruit and fruit stones enabled reconstruction of the conditions and state in which the fruit stones burned. The results suggest that Cornelian cherry fruit could have had a symbolic role in the complex funerary ritual performed by the Mesolithic community, a role that was meant to ensure the memory of, and a link with, the deceased. This adds to the current understanding of multiple potential uses of wild plants in the past and, excitingly, points to their role in mortuary practices as early as the Mesolithic.
T2  - Quaternary International
T1  - Gathered fruits as grave goods? Cornelian cherry remains from a Mesolithic grave at the site of Vlasac, Danube Gorges, south-east Europe
DO  - 10.1016/j.quaint.2019.10.018
author = "Filipović, Dragana and Fotirić-Akšić, Milica M. and Dabić-Zagorac, Dragana and Natić, Maja",
abstract = "Establishing the use and purpose of wild plant remains recovered from archaeological layers is in many cases far from straightforward. When discovered in a mortuary context, they are generally interpreted as remnants of ritual eating or drinking, offerings to the dead, or mnemonics, if not as accidental inclusions in the grave fills. In the Mesolithic cremation graves at the site of Vlasac in the Danube Gorges, south-east Europe, Cornelian cherry (Cornus mas L.) fruit stones were discovered; in one case, their number was relatively high. Whereas the fruit stones could simply be regarded as contamination in the burial fill, this study investigates an alternative possibility – that the cherries, or just fruit stones, represented an element of the Mesolithic funerary ritual at Vlasac. The archaeological context in which this plant material was discovered is taken as fundamental to the understanding of taphonomy of the botanical evidence and so it was considered in detail. Examination of the relevant morphological properties and the experimental charring of modern Cornelian cherry fruit and fruit stones enabled reconstruction of the conditions and state in which the fruit stones burned. The results suggest that Cornelian cherry fruit could have had a symbolic role in the complex funerary ritual performed by the Mesolithic community, a role that was meant to ensure the memory of, and a link with, the deceased. This adds to the current understanding of multiple potential uses of wild plants in the past and, excitingly, points to their role in mortuary practices as early as the Mesolithic.",
journal = "Quaternary International",
title = "Gathered fruits as grave goods? Cornelian cherry remains from a Mesolithic grave at the site of Vlasac, Danube Gorges, south-east Europe",
doi = "10.1016/j.quaint.2019.10.018"
Filipović, D., Fotirić-Akšić, M. M., Dabić-Zagorac, D.,& Natić, M.. (2020). Gathered fruits as grave goods? Cornelian cherry remains from a Mesolithic grave at the site of Vlasac, Danube Gorges, south-east Europe. in Quaternary International
https://doi.org/10.1016/j.quaint.2019.10.018
Filipović D, Fotirić-Akšić MM, Dabić-Zagorac D, Natić M. Gathered fruits as grave goods? Cornelian cherry remains from a Mesolithic grave at the site of Vlasac, Danube Gorges, south-east Europe. in Quaternary International. 2020;541:130-140.
doi:10.1016/j.quaint.2019.10.018 .
Filipović, Dragana, Fotirić-Akšić, Milica M., Dabić-Zagorac, Dragana, Natić, Maja, "Gathered fruits as grave goods? Cornelian cherry remains from a Mesolithic grave at the site of Vlasac, Danube Gorges, south-east Europe" in Quaternary International, 541 (2020):130-140,
https://doi.org/10.1016/j.quaint.2019.10.018 . .
Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value
Milinčić, Danijel D.; Kostić, Aleksandar Ž.; Špirović-Trifunović, Bojana ; Tešić, Živoslav Lj.; Tosti, Tomislav; Dramićanin, Aleksandra M.; Barać, Miroljub B.; Pešić, Mirjana B.
AU  - Špirović-Trifunović, Bojana
AB  - The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly ω-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.
T1  - Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value
DO  - 10.2298/JSC190713117M
author = "Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Špirović-Trifunović, Bojana  and Tešić, Živoslav Lj. and Tosti, Tomislav and Dramićanin, Aleksandra M. and Barać, Miroljub B. and Pešić, Mirjana B.",
abstract = "The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly ω-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.",
title = "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value",
doi = "10.2298/JSC190713117M"
Milinčić, D. D., Kostić, A. Ž., Špirović-Trifunović, B., Tešić, Ž. Lj., Tosti, T., Dramićanin, A. M., Barać, M. B.,& Pešić, M. B.. (2020). Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in Journal of the Serbian Chemical Society
https://doi.org/10.2298/JSC190713117M
Milinčić DD, Kostić AŽ, Špirović-Trifunović B, Tešić ŽL, Tosti T, Dramićanin AM, Barać MB, Pešić MB. Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in Journal of the Serbian Chemical Society. 2020;85(3):305-319.
doi:10.2298/JSC190713117M .
Milinčić, Danijel D., Kostić, Aleksandar Ž., Špirović-Trifunović, Bojana , Tešić, Živoslav Lj., Tosti, Tomislav, Dramićanin, Aleksandra M., Barać, Miroljub B., Pešić, Mirjana B., "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value" in Journal of the Serbian Chemical Society, 85, no. 3 (2020):305-319,
https://doi.org/10.2298/JSC190713117M . .
Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid
Katanić-Stanković, Jelena; Srećković, Nikola; Mišić, Danijela; Gašić, Uroš M.; Imbimbo, Paola; Monti, Daria M.; Mihailović, Vladimir
AU  - Katanić-Stanković, Jelena
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Imbimbo, Paola
AU  - Monti, Daria M.
AU  - Mihailović, Vladimir
AB  - The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.
T2  - Industrial Crops and Products
T1  - Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid
DO  - 10.1016/j.indcrop.2019.111932
author = "Katanić-Stanković, Jelena and Srećković, Nikola and Mišić, Danijela and Gašić, Uroš M. and Imbimbo, Paola and Monti, Daria M. and Mihailović, Vladimir",
abstract = "The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.",
journal = "Industrial Crops and Products",
title = "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid",
doi = "10.1016/j.indcrop.2019.111932"
Katanić-Stanković, J., Srećković, N., Mišić, D., Gašić, U. M., Imbimbo, P., Monti, D. M.,& Mihailović, V.. (2020). Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products
https://doi.org/10.1016/j.indcrop.2019.111932
Katanić-Stanković J, Srećković N, Mišić D, Gašić UM, Imbimbo P, Monti DM, Mihailović V. Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products. 2020;143:111932.
doi:10.1016/j.indcrop.2019.111932 .
Katanić-Stanković, Jelena, Srećković, Nikola, Mišić, Danijela, Gašić, Uroš M., Imbimbo, Paola, Monti, Daria M., Mihailović, Vladimir, "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid" in Industrial Crops and Products, 143 (2020):111932,
https://doi.org/10.1016/j.indcrop.2019.111932 . .
Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro
Nešović, Milica; Gašić, Uroš M.; Tosti, Tomislav; Trifković, Jelena; Baošić, Rada; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav Lj.
AB  - The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.
T1  - Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro
DO  - 10.1039/c9ra08783d
author = "Nešović, Milica and Gašić, Uroš M. and Tosti, Tomislav and Trifković, Jelena and Baošić, Rada and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav Lj.",
abstract = "The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.",
title = "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro",
doi = "10.1039/c9ra08783d"
Nešović, M., Gašić, U. M., Tosti, T., Trifković, J., Baošić, R., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2020). Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances
Royal Society of Chemistry., 10(5), 2462-2471.
https://doi.org/10.1039/c9ra08783d
Nešović M, Gašić UM, Tosti T, Trifković J, Baošić R, Blagojević S, Ignjatović L, Tešić ŽL. Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances. 2020;10(5):2462-2471.
doi:10.1039/c9ra08783d .
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Trifković, Jelena, Baošić, Rada, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro" in RSC Advances, 10, no. 5 (2020):2462-2471,
https://doi.org/10.1039/c9ra08783d . .
Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d
T1  - Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d
title = "Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d",
Nešović, M., Gašić, U. M., Tosti, T., Trifković, J., Baošić, R., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2020). Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d. in RSC Advances
Nešović M, Gašić UM, Tosti T, Trifković J, Baošić R, Blagojević S, Ignjatović L, Tešić ŽL. Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d. in RSC Advances. 2020;.
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Trifković, Jelena, Baošić, Rada, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d" in RSC Advances (2020),
Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro
Šuković, Danijela; Knežević, Bojana; Gašić, Uroš M.; Sredojević, Milica; Ćirić, Ivanka; Todić, Slavica; Mutić, Jelena; Tešić, Živoslav Lj.
AU  - Šuković, Danijela
AU  - Knežević, Bojana
AU  - Todić, Slavica
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
T1  - Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro
author = "Šuković, Danijela and Knežević, Bojana and Gašić, Uroš M. and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav Lj.",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
title = "Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro",
Šuković, D., Knežević, B., Gašić, U. M., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž. Lj.. (2020). Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro. in Foods
MDPI., 9(2), 1-24.
Šuković D, Knežević B, Gašić UM, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić ŽL. Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro. in Foods. 2020;9(2):1-24.
Šuković, Danijela, Knežević, Bojana, Gašić, Uroš M., Sredojević, Milica, Ćirić, Ivanka, Todić, Slavica, Mutić, Jelena, Tešić, Živoslav Lj., "Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro" in Foods, 9, no. 2 (2020):1-24,
Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics
Vasić, Vesna; Đurđić, Slađana Z.; Tosti, Tomislav; Radoičić, Aleksandra; Lušić, Dražen; Milojković-Opsenica, Dušanka; Tešić, Živoslav Lj.; Trifković, Jelena
AU  - Vasić, Vesna
AU  - Đurđić, Slađana Z.
AU  - Radoičić, Aleksandra
AU  - Lušić, Dražen
AB  - Taking into account a growing market and small number of articles related to honeydew honey, a metabolomic approach associated with multivariate analysis and modelling was proposed to discriminate five varieties of honey. Advanced analytical techniques were used for determination of 20 elements, 14 carbohydrates and stable carbon isotope ratio. No chemical marker has been found within sugar compounds, but several elements (Ba, Ca, Mg, Sr, Mn, Al, Co, Ni, Se) were marked as characteristic of honey type and allow classification of three botanical origins (Abies alba, Quercus frainetto, Quercus ilex). Sugars turanose, trehalose, arabinose and raffinose, elements Ba, Sr, P, Cd and Se, and δ13C values of honey, have different concentrations in honeys of the same botanical origin but harvested in different season. In addition to a confirmation of authenticity in terms of production, the values of δ13C of protein could be a good indicator of botanical origin.
T1  - Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics
author = "Vasić, Vesna and Đurđić, Slađana Z. and Tosti, Tomislav and Radoičić, Aleksandra and Lušić, Dražen and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj. and Trifković, Jelena",
abstract = "Taking into account a growing market and small number of articles related to honeydew honey, a metabolomic approach associated with multivariate analysis and modelling was proposed to discriminate five varieties of honey. Advanced analytical techniques were used for determination of 20 elements, 14 carbohydrates and stable carbon isotope ratio. No chemical marker has been found within sugar compounds, but several elements (Ba, Ca, Mg, Sr, Mn, Al, Co, Ni, Se) were marked as characteristic of honey type and allow classification of three botanical origins (Abies alba, Quercus frainetto, Quercus ilex). Sugars turanose, trehalose, arabinose and raffinose, elements Ba, Sr, P, Cd and Se, and δ13C values of honey, have different concentrations in honeys of the same botanical origin but harvested in different season. In addition to a confirmation of authenticity in terms of production, the values of δ13C of protein could be a good indicator of botanical origin.",
title = "Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics",
Vasić, V., Đurđić, S. Z., Tosti, T., Radoičić, A., Lušić, D., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Trifković, J.. (2020). Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics. in Food Chemistry, 305.
Vasić V, Đurđić SZ, Tosti T, Radoičić A, Lušić D, Milojković-Opsenica D, Tešić ŽL, Trifković J. Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics. in Food Chemistry. 2020;305.
Vasić, Vesna, Đurđić, Slađana Z., Tosti, Tomislav, Radoičić, Aleksandra, Lušić, Dražen, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., Trifković, Jelena, "Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics" in Food Chemistry, 305 (2020),
Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457
T1  - Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457
title = "Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457",
Vasić, V., Đurđić, S. Z., Tosti, T., Radoičić, A., Lušić, D., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Trifković, J.. (2020). Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457. in Food Chemistry.
Vasić V, Đurđić SZ, Tosti T, Radoičić A, Lušić D, Milojković-Opsenica D, Tešić ŽL, Trifković J. Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457. in Food Chemistry. 2020;.
Vasić, Vesna, Đurđić, Slađana Z., Tosti, Tomislav, Radoičić, Aleksandra, Lušić, Dražen, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., Trifković, Jelena, "Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457" in Food Chemistry (2020),
Recent trends in image evaluation of HPTLC chromatograms
Ristivojević, Petar; Trifković, Jelena; Andrić, Filip; Milojković-Opsenica, Dušanka
(Marcel Dekker, 2020)
AB  - In the current article, an overview of recent applications and opportunities of image evaluation of
high-performance thin-layer chromatograms (HPTLC) in food analysis and natural product
research is presented. The article shortly covers the aspects of specialized software packages for
image analysis, image post-correction, signal acquisition, and preprocessing. Contemporary aspects
of image-based HPTLC fingerprinting, quantification of target compounds, and advanced chemometric
modeling were reviewed. Special attention was dedicated to freely available software packages.
Advantages and disadvantages of each one were discussed in terms of abilities to obtain
chromatographic profiles and perform crucial steps in signal manipulation, such as background
subtraction, denoising, and background detrending. The most frequently applied techniques for
signal manipulation have been discussed and recommendation provided. In that sense, the article
aims to provide a valuable guideline for readers dealing with the application of HPTLC image analysis
in food and natural products research, especially in connection with the most frequently used
chemometric techniques, in domains of pattern recognition, classification, and regression.
PB  - Marcel Dekker
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Recent trends in image evaluation of HPTLC chromatograms
author = "Ristivojević, Petar and Trifković, Jelena and Andrić, Filip and Milojković-Opsenica, Dušanka",
abstract = "In the current article, an overview of recent applications and opportunities of image evaluation of
chemometric techniques, in domains of pattern recognition, classification, and regression.",
publisher = "Marcel Dekker",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Recent trends in image evaluation of HPTLC chromatograms",
Ristivojević, P., Trifković, J., Andrić, F.,& Milojković-Opsenica, D.. (2020). Recent trends in image evaluation of HPTLC chromatograms. in Journal of Liquid Chromatography & Related Technologies
Marcel Dekker., 43, 291-299.
Ristivojević P, Trifković J, Andrić F, Milojković-Opsenica D. Recent trends in image evaluation of HPTLC chromatograms. in Journal of Liquid Chromatography & Related Technologies. 2020;43:291-299.
Ristivojević, Petar, Trifković, Jelena, Andrić, Filip, Milojković-Opsenica, Dušanka, "Recent trends in image evaluation of HPTLC chromatograms" in Journal of Liquid Chromatography & Related Technologies, 43 (2020):291-299,
Assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem
Sarap, Nataša B.; Krneta Nikolić, Jelena D.; Trifković, Jelena; Janković, Marija M.
AU  - Sarap, Nataša B.
AU  - Krneta Nikolić, Jelena D.
AU  - Janković, Marija M.
AB  - Taking into account the importance of distribution and transfer of radionuclides in the soil–plant system, especially in agricultural fields, the aim of this research was assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem. The measurement of the natural radioactivity distribution was conducted in organic and sustainable farming management systems. Statistical evaluation of the physical and chemical properties of soils subjected to different farming systems, revealed the existence of differences in some parameters. Balanced distribution of natural radionuclides in soil-crop system was observed. Radionuclide 40K was the most accessible to the investigated crops via uptake from the soil, then 232Th and 226Ra. The obtained results are useful for understanding the behavior of the radionuclides and provide an insight into the cumulative deposition of radioactivity in the agroecosystem.
T2  - Journal of Radioanalytical and Nuclear Chemistry
T1  - Assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem
author = "Sarap, Nataša B. and Krneta Nikolić, Jelena D. and Trifković, Jelena and Janković, Marija M.",
abstract = "Taking into account the importance of distribution and transfer of radionuclides in the soil–plant system, especially in agricultural fields, the aim of this research was assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem. The measurement of the natural radioactivity distribution was conducted in organic and sustainable farming management systems. Statistical evaluation of the physical and chemical properties of soils subjected to different farming systems, revealed the existence of differences in some parameters. Balanced distribution of natural radionuclides in soil-crop system was observed. Radionuclide 40K was the most accessible to the investigated crops via uptake from the soil, then 232Th and 226Ra. The obtained results are useful for understanding the behavior of the radionuclides and provide an insight into the cumulative deposition of radioactivity in the agroecosystem.",
journal = "Journal of Radioanalytical and Nuclear Chemistry",
title = "Assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem",
Sarap, N. B., Krneta Nikolić, J. D., Trifković, J.,& Janković, M. M.. (2020). Assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem. in Journal of Radioanalytical and Nuclear Chemistry
Springer., 323(2), 805-815.
Sarap NB, Krneta Nikolić JD, Trifković J, Janković MM. Assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem. in Journal of Radioanalytical and Nuclear Chemistry. 2020;323(2):805-815.
Sarap, Nataša B., Krneta Nikolić, Jelena D., Trifković, Jelena, Janković, Marija M., "Assessment of radioactivity contribution and transfer characteristics of natural radionuclides in agroecosystem" in Journal of Radioanalytical and Nuclear Chemistry, 323, no. 2 (2020):805-815,
Extraction as a Critical Step in Phytochemical Analysis
Mosić, Mirjana D.; Dramićanin, Aleksandra M.; Ristivojević, Petar; Milojković-Opsenica, Dušanka
(Oxford University Press, 2020)
AB  - Phytochemical analysis, the chemical investigation of plants, is a rapidly developing chemical discipline with various goals such as the determination of the substance classes and the isolation and qualitative and/or quantitative analyses of bioactive compounds or their structural analysis. The analysis of plants begins with optimizing extraction procedures because they play a key role in extraction outcomes, i.e., the isolation of bioactive compounds from natural products and the selection of the subsequent tests used in the analyses. The extraction is a critical step because it is necessary to extract the desired components of the complex natural matrix without destroying them. A wide range of techniques for the extraction of bioactive compounds from natural matrixes are currently available. This review aims to describe and compare the most commonly used methods, traditional/conventional and innovative/green. It will focus on the principles behind each method and on their strengths and limitations in order to help evaluate their suitability and economic feasibility, highlighting the advantages of new innovative techniques over conventional extraction procedures. Finally, the recent application of deep eutectic solvents as a new, eco-friendly method for the extraction of bioactive compounds from natural matrixes is also discussed.
PB  - Oxford University Press
T2  - Journal of AOAC International
T1  - Extraction as a Critical Step in Phytochemical Analysis
DO  - 10.5740/jaoacint.19-0251
author = "Mosić, Mirjana D. and Dramićanin, Aleksandra M. and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
abstract = "Phytochemical analysis, the chemical investigation of plants, is a rapidly developing chemical discipline with various goals such as the determination of the substance classes and the isolation and qualitative and/or quantitative analyses of bioactive compounds or their structural analysis. The analysis of plants begins with optimizing extraction procedures because they play a key role in extraction outcomes, i.e., the isolation of bioactive compounds from natural products and the selection of the subsequent tests used in the analyses. The extraction is a critical step because it is necessary to extract the desired components of the complex natural matrix without destroying them. A wide range of techniques for the extraction of bioactive compounds from natural matrixes are currently available. This review aims to describe and compare the most commonly used methods, traditional/conventional and innovative/green. It will focus on the principles behind each method and on their strengths and limitations in order to help evaluate their suitability and economic feasibility, highlighting the advantages of new innovative techniques over conventional extraction procedures. Finally, the recent application of deep eutectic solvents as a new, eco-friendly method for the extraction of bioactive compounds from natural matrixes is also discussed.",
journal = "Journal of AOAC International",
title = "Extraction as a Critical Step in Phytochemical Analysis",
doi = "10.5740/jaoacint.19-0251"
Mosić, M. D., Dramićanin, A. M., Ristivojević, P.,& Milojković-Opsenica, D.. (2020). Extraction as a Critical Step in Phytochemical Analysis. in Journal of AOAC International
Oxford University Press., 103.
https://doi.org/10.5740/jaoacint.19-0251
Mosić MD, Dramićanin AM, Ristivojević P, Milojković-Opsenica D. Extraction as a Critical Step in Phytochemical Analysis. in Journal of AOAC International. 2020;103.
doi:10.5740/jaoacint.19-0251 .
Mosić, Mirjana D., Dramićanin, Aleksandra M., Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Extraction as a Critical Step in Phytochemical Analysis" in Journal of AOAC International, 103 (2020),
https://doi.org/10.5740/jaoacint.19-0251 . .
Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species
Ristivojević, Petar; Stević, Tatjana; Starović, M.; Pavlović, Slađan Z.; Özcan, M.M.; Berić, T.; Dimkić, Ivica
AU  - Stević, Tatjana
AU  - Starović, M.
AU  - Pavlović, Slađan Z.
AU  - Özcan, M.M.
AU  - Berić, T.
AB  - Aims: A multidisciplinary approach was used to compare phenolic composition, radical scavenging and antimicrobial activity of propolis samples from different geographical localities, and plant resin against various microorganisms. Methods and Results: Using UHPLC-qqqMS quantitative analysis, 28 phenolic compounds were determined. Caffeic and p-coumaric acids were identified as main phenolic acids in poplar propolis samples, except samples from Russia (P6) and China (P7). Radical scavenging activity (applying DPPH spectrophotometric assay) showed the highest activity of Serbian (40·51%) and Chinese (53·21%) propolis samples. Broth microdilution method was used for the oral cavity, fungal phytopathogenic and human vaginal isolates which have been identified at a molecular level. The most sensitive bacterial isolates were Lactobacillus acidophilus (MIC of 0·03–0·13 mg ml−1) and the oral streptococci isolates (MIC values of 0·19–0·13 mg ml−1). The most sensitive fungal phytopathogenic isolate was Fusarium oxysporum (MIC 0·003 mg ml−1). All samples, except propolis from Serbia (P4) and Turkey (P5), showed a strong antifungal activity against Fusarium sporotrichioides, Fusarium subglutinans and Fusarium proliferatum. Conclusion: The results of various tests indicate good radical scavenging and antimicrobial activity against important human and plant pathogens. Significance and Impact of the Study: A detailed propolis analysis is important when proposing a preparation of new biological antimicrobial products which have a positive impact on human health and reduce antibacterial resistance.
T2  - Journal of Applied Microbiology
T1  - Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species
DO  - 10.1111/jam.14633
author = "Ristivojević, Petar and Stević, Tatjana and Starović, M. and Pavlović, Slađan Z. and Özcan, M.M. and Berić, T. and Dimkić, Ivica",
abstract = "Aims: A multidisciplinary approach was used to compare phenolic composition, radical scavenging and antimicrobial activity of propolis samples from different geographical localities, and plant resin against various microorganisms. Methods and Results: Using UHPLC-qqqMS quantitative analysis, 28 phenolic compounds were determined. Caffeic and p-coumaric acids were identified as main phenolic acids in poplar propolis samples, except samples from Russia (P6) and China (P7). Radical scavenging activity (applying DPPH spectrophotometric assay) showed the highest activity of Serbian (40·51%) and Chinese (53·21%) propolis samples. Broth microdilution method was used for the oral cavity, fungal phytopathogenic and human vaginal isolates which have been identified at a molecular level. The most sensitive bacterial isolates were Lactobacillus acidophilus (MIC of 0·03–0·13 mg ml−1) and the oral streptococci isolates (MIC values of 0·19–0·13 mg ml−1). The most sensitive fungal phytopathogenic isolate was Fusarium oxysporum (MIC 0·003 mg ml−1). All samples, except propolis from Serbia (P4) and Turkey (P5), showed a strong antifungal activity against Fusarium sporotrichioides, Fusarium subglutinans and Fusarium proliferatum. Conclusion: The results of various tests indicate good radical scavenging and antimicrobial activity against important human and plant pathogens. Significance and Impact of the Study: A detailed propolis analysis is important when proposing a preparation of new biological antimicrobial products which have a positive impact on human health and reduce antibacterial resistance.",
journal = "Journal of Applied Microbiology",
title = "Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species",
doi = "10.1111/jam.14633"
Ristivojević, P., Stević, T., Starović, M., Pavlović, S. Z., Özcan, M.M., Berić, T.,& Dimkić, I.. (2020). Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species. in Journal of Applied Microbiology, 129(2), 296-310.
https://doi.org/10.1111/jam.14633
Ristivojević P, Stević T, Starović M, Pavlović SZ, Özcan M, Berić T, Dimkić I. Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species. in Journal of Applied Microbiology. 2020;129(2):296-310.
doi:10.1111/jam.14633 .
Ristivojević, Petar, Stević, Tatjana, Starović, M., Pavlović, Slađan Z., Özcan, M.M., Berić, T., Dimkić, Ivica, "Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species" in Journal of Applied Microbiology, 129, no. 2 (2020):296-310,
https://doi.org/10.1111/jam.14633 . .
The phenolic profile of strawberry tree (Arbutus unedo L.) honey
Jurič, Andreja; Gašić, Gašić; Brčić-Karačonji, Irena; Jurica, Karlo; Milojković-Opsenica, Dušanka
AU  - Jurič, Andreja
AU  - Gašić, Gašić
AB  - Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food.
T1  - The phenolic profile of strawberry tree (Arbutus unedo L.) honey
DO  - 10.2298/JSC191217018J
author = "Jurič, Andreja and Gašić, Gašić and Brčić-Karačonji, Irena and Jurica, Karlo and Milojković-Opsenica, Dušanka",
abstract = "Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food.",
title = "The phenolic profile of strawberry tree (Arbutus unedo L.) honey",
doi = "10.2298/JSC191217018J"
Jurič, A., Gašić, G., Brčić-Karačonji, I., Jurica, K.,& Milojković-Opsenica, D.. (2020). The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 85(3), 1-10.
https://doi.org/10.2298/JSC191217018J
Jurič A, Gašić G, Brčić-Karačonji I, Jurica K, Milojković-Opsenica D. The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society. 2020;85(3):1-10.
doi:10.2298/JSC191217018J .
Jurič, Andreja, Gašić, Gašić, Brčić-Karačonji, Irena, Jurica, Karlo, Milojković-Opsenica, Dušanka, "The phenolic profile of strawberry tree (Arbutus unedo L.) honey" in Journal of the Serbian Chemical Society, 85, no. 3 (2020):1-10,
https://doi.org/10.2298/JSC191217018J . .