Suppression of insulin secretion by C-peptide infusion in humans
P. Cohen, Nir Barzilai, E. Karnieli
A role for insulin in the regulation of its own secretion has been established; however, no such effect of C-peptide has been demonstrated. In order to reexamine the role of C-peptide and insulin in regulating β cell secretion, we infused C-peptide as a primed continuous infusion of 1 ng/min per kg for 45 min to 7 healthy volunteers, and insulin in a similar manner at rates of 1 and 10 mU/min per kg for 2 h to 14 healthy subjects using the euglycemic insulin clamp technique. Plasma insulin and C-peptide were measured before and during the infusions. During C-peptide infusion, C-peptide levels rose from 1.8 ± 0.2 to 2.3 ± 0.2 ng/ml; and insulin levels fell from 14.5 ± 0.8 to 11.0 ± 1.0 μU/ml (P < 0.01). During low and high rate insulin infusions, insulin levels rose to 70 ± 8 and 1,020 ± 105 μU/ml, while C-peptide levels fell significantly from 1.9 ± 0.2 to 1.5 ± 0.2 and to 1.3 ± 0.1 ng/ml, respectively. Thus 5- and 70-fold increases in circulating insulin levels caused 15% and 33% drops in serum C-peptide, respectively, However, a 30% increase in C-peptide levels caused a significant ~ 24% decrease in the levels of plasma insulin. We conclude that insulin induces a dose-dependent inhibition of β cell secretion, which is even more sensitive to inhibition by C-peptide. These data suggest a physiological role for C-peptide in regulating human insulin secretion in vivo.
Israel Journal of Medical Sciences
Glucose Clamp Technique
Euglycemic clamp technique
Insulin secretion
Cohen, P., Barzilai, N., & Karnieli, E. (1995). Suppression of insulin secretion by C-peptide infusion in humans. Israel Journal of Medical Sciences, 31(5), 284-288.
Suppression of insulin secretion by C-peptide infusion in humans. / Cohen, P.; Barzilai, Nir; Karnieli, E.
In: Israel Journal of Medical Sciences, Vol. 31, No. 5, 1995, p. 284-288.
Cohen, P, Barzilai, N & Karnieli, E 1995, 'Suppression of insulin secretion by C-peptide infusion in humans', Israel Journal of Medical Sciences, vol. 31, no. 5, pp. 284-288.
Cohen P, Barzilai N, Karnieli E. Suppression of insulin secretion by C-peptide infusion in humans. Israel Journal of Medical Sciences. 1995;31(5):284-288.
Cohen, P. ; Barzilai, Nir ; Karnieli, E. / Suppression of insulin secretion by C-peptide infusion in humans. In: Israel Journal of Medical Sciences. 1995 ; Vol. 31, No. 5. pp. 284-288.
@article{b0e83d7ae2ec403b910dadf0f502f8fd,
title = "Suppression of insulin secretion by C-peptide infusion in humans",
abstract = "A role for insulin in the regulation of its own secretion has been established; however, no such effect of C-peptide has been demonstrated. In order to reexamine the role of C-peptide and insulin in regulating β cell secretion, we infused C-peptide as a primed continuous infusion of 1 ng/min per kg for 45 min to 7 healthy volunteers, and insulin in a similar manner at rates of 1 and 10 mU/min per kg for 2 h to 14 healthy subjects using the euglycemic insulin clamp technique. Plasma insulin and C-peptide were measured before and during the infusions. During C-peptide infusion, C-peptide levels rose from 1.8 ± 0.2 to 2.3 ± 0.2 ng/ml; and insulin levels fell from 14.5 ± 0.8 to 11.0 ± 1.0 μU/ml (P < 0.01). During low and high rate insulin infusions, insulin levels rose to 70 ± 8 and 1,020 ± 105 μU/ml, while C-peptide levels fell significantly from 1.9 ± 0.2 to 1.5 ± 0.2 and to 1.3 ± 0.1 ng/ml, respectively. Thus 5- and 70-fold increases in circulating insulin levels caused 15{\%} and 33{\%} drops in serum C-peptide, respectively, However, a 30{\%} increase in C-peptide levels caused a significant ~ 24{\%} decrease in the levels of plasma insulin. We conclude that insulin induces a dose-dependent inhibition of β cell secretion, which is even more sensitive to inhibition by C-peptide. These data suggest a physiological role for C-peptide in regulating human insulin secretion in vivo.",
keywords = "C-peptide, Euglycemic clamp technique, Insulin, Insulin secretion",
author = "P. Cohen and Nir Barzilai and E. Karnieli",
journal = "Israel Medical Association Journal",
publisher = "Israel Medical Association",
T1 - Suppression of insulin secretion by C-peptide infusion in humans
AU - Cohen, P.
AU - Barzilai, Nir
AU - Karnieli, E.
N2 - A role for insulin in the regulation of its own secretion has been established; however, no such effect of C-peptide has been demonstrated. In order to reexamine the role of C-peptide and insulin in regulating β cell secretion, we infused C-peptide as a primed continuous infusion of 1 ng/min per kg for 45 min to 7 healthy volunteers, and insulin in a similar manner at rates of 1 and 10 mU/min per kg for 2 h to 14 healthy subjects using the euglycemic insulin clamp technique. Plasma insulin and C-peptide were measured before and during the infusions. During C-peptide infusion, C-peptide levels rose from 1.8 ± 0.2 to 2.3 ± 0.2 ng/ml; and insulin levels fell from 14.5 ± 0.8 to 11.0 ± 1.0 μU/ml (P < 0.01). During low and high rate insulin infusions, insulin levels rose to 70 ± 8 and 1,020 ± 105 μU/ml, while C-peptide levels fell significantly from 1.9 ± 0.2 to 1.5 ± 0.2 and to 1.3 ± 0.1 ng/ml, respectively. Thus 5- and 70-fold increases in circulating insulin levels caused 15% and 33% drops in serum C-peptide, respectively, However, a 30% increase in C-peptide levels caused a significant ~ 24% decrease in the levels of plasma insulin. We conclude that insulin induces a dose-dependent inhibition of β cell secretion, which is even more sensitive to inhibition by C-peptide. These data suggest a physiological role for C-peptide in regulating human insulin secretion in vivo.
AB - A role for insulin in the regulation of its own secretion has been established; however, no such effect of C-peptide has been demonstrated. In order to reexamine the role of C-peptide and insulin in regulating β cell secretion, we infused C-peptide as a primed continuous infusion of 1 ng/min per kg for 45 min to 7 healthy volunteers, and insulin in a similar manner at rates of 1 and 10 mU/min per kg for 2 h to 14 healthy subjects using the euglycemic insulin clamp technique. Plasma insulin and C-peptide were measured before and during the infusions. During C-peptide infusion, C-peptide levels rose from 1.8 ± 0.2 to 2.3 ± 0.2 ng/ml; and insulin levels fell from 14.5 ± 0.8 to 11.0 ± 1.0 μU/ml (P < 0.01). During low and high rate insulin infusions, insulin levels rose to 70 ± 8 and 1,020 ± 105 μU/ml, while C-peptide levels fell significantly from 1.9 ± 0.2 to 1.5 ± 0.2 and to 1.3 ± 0.1 ng/ml, respectively. Thus 5- and 70-fold increases in circulating insulin levels caused 15% and 33% drops in serum C-peptide, respectively, However, a 30% increase in C-peptide levels caused a significant ~ 24% decrease in the levels of plasma insulin. We conclude that insulin induces a dose-dependent inhibition of β cell secretion, which is even more sensitive to inhibition by C-peptide. These data suggest a physiological role for C-peptide in regulating human insulin secretion in vivo.
KW - C-peptide
KW - Euglycemic clamp technique
KW - Insulin
KW - Insulin secretion
JO - Israel Medical Association Journal
JF - Israel Medical Association Journal