PDBe › 1h2m
Factor Inhibiting HIF-1 alpha in complex with HIF-1 alpha fragment peptide
DOI: 10.2210/pdb1h2m/pdb
Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha.
Elkins JM, Hewitson KS, McNeill LA, Seibel JF, Schlemminger I, Pugh CW, Ratcliffe PJ, Schofield CJ
J. Biol. Chem. 278 1802-6 (2003)
Reactions catalysed:
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
peptidyl-histidine hydroxylation
cytosol
JmjC domain
Cupin-like domain 8
Hypoxia-inducible factor 1-alpha inhibitor, domain II
HIF-1 alpha, transactivation domain, C-terminal
Hypoxia-inducible factor 1-alpha inhibitor
Structure domain:
Hypoxia-inducible factor HIF ihhibitor (FIH1)
Non-polymer only tetramer (preferred)
2 distinct polypeptide molecules
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Expression system: Escherichia coli BL21(DE3)
Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Clavaminate synthase-like
Hypoxia-inducible factor 1-alpha Chain: S
Chain: S
Length: 52 amino acids
Canonical: Q16665 (Residues: 775-826; Coverage: 6%)
Gene names: BHLHE78, HIF1A, MOP1, PASD8
Sequence domains: HIF-1 alpha C terminal transactivation domain
1 x ZN
1 x OGA
2 x SO4
X-ray source: SRS BEAMLINE PX9.6
a: 86.249Å b: 86.249Å c: 148.26Å
1h2m overview
1h2m @ RCSB
1h2m @ PDBj
1h2m @ PDBSum
1h2m @ Proteopedia
69 review citations
Energy metabolism in anaerobic eukaryotes and Earth's late oxygenation.
Zimorski et al. (2019)
Structural characterization of mammalian bHLH-PAS transcription factors.
Wu et al. (2017)